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Martha E Trujillo - One of the best experts on this subject based on the ideXlab platform.
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genome sequence of micromonospora lupini lupac 08 isolated from Root Nodules of lupinus angustifolius
Journal of Bacteriology, 2012Co-Authors: Pablo Alonsovega, Lorena Carro, Petar Pujic, Rodrigo Bacigalupe, Aurelie Lajus, David Vallenet, Pedro M Coll, Martha E TrujilloAbstract:Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and Root Nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from Root Nodules of the wild legume Lupinus angustifolious.
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the genus micromonospora is widespread in legume Root Nodules the example of lupinus angustifolius
The ISME Journal, 2010Co-Authors: Martha E Trujillo, Lorena Carro, Pablo Alonsovega, Raul Rodriguez, Eugenia Cerda, Pilar Alonso, Eustoquio MartinezmolinaAbstract:Our current knowledge of plant-microbe interactions indicate that populations inhabiting a host plant are not restricted to a single microbial species but comprise several genera and species. No one knows if communities inside plants interact, and it has been speculated that beneficial effects are the result of their combined activities. During an ecological study of nitrogen-fixing bacterial communities from Lupinus angustifolius collected in Spain, significant numbers of orange-pigmented actinomycete colonies were isolated from surface-sterilized Root Nodules. The isolates were analysed by BOX-PCR fingerprinting revealing an unexpectedly high genetic variation. Selected strains were chosen for 16S rRNA gene sequencing and phylogenetic analyses confirmed that all strains isolated belonged to the genus Micromonospora and that some of them may represent new species. To determine the possibility that the isolates fixed atmospheric nitrogen, chosen strains were grown in nitrogen-free media, obtaining in some cases, significant growth when compared with the controls. These strains were further screened for the presence of the nifH gene encoding dinitrogenase reductase, a key enzyme in nitrogen fixation. The partial nifH-like gene sequences obtained showed a 99% similarity with the sequence of the nifH gene from Frankia alni ACN14a, an actinobacterium that induces nodulation and fixes nitrogen in symbiosis with Alnus. In addition, in situ hybridization was performed to determine if these microorganisms inhabit the inside of the Nodules. This study strongly suggests that Micromonospora populations are natural inhabitants of nitrogen-fixing Root Nodules.
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Micromonospora pisi sp. nov., isolated from Root Nodules of Pisum sativum.
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Lorena Carro Garcia, Eustoquio Martínez-molina, Martha E TrujilloAbstract:A novel actinomycete, designated strain GUI 15T, isolated from the Root Nodules of a Pisum sativum plant was characterized taxonomically by using a polyphasic approach. The 16S rRNA gene sequence of strain GUI 15T showed highest similarity to Micromonospora pattaloongensis TJ2-2T (98.7 %) and Polymorphospora rubra TT 97-42T (98.5 %). Phylogenetic analysis based on the gyrase B gene also supported the close relationship of these three strains, but indicated that strain GUI 15T should be assigned to the genus Micromonospora. Chemotaxonomic results confirmed the position of the isolate in the genus Micromonospora, but revealed differences at the species level. The novel strain could be distinguished from recognized Micromonospora species by using a combination of physiological and biochemical tests. Based on these observations, strain GUI 15T is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora pisi sp. nov. is proposed. The type strain is GUI 15T (=DSM 45175T=LMG 24546T).
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Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from Root Nodules of Lupinus angustifolius.
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Martha E Trujillo, Reiner M Kroppenstedt, Peter Schumann, Carmen Fernández-molinero, Eustoquio Martínez-molinaAbstract:A study was conducted to determine the taxonomic status of six actinomycete strains isolated from Root Nodules of Lupinus angustifolius. The strains were filamentous, Gram-positive and produced single spores at the tip of the hyphae. Phylogenetic, chemotaxonomic and morphological analyses demonstrated that all six strains belonged to the genus Micromonospora. According to the 16S rRNA gene sequence data, the strains were divided into two clusters that are moderately related to Micromonospora mirobrigensis, Micromonospora matsumotoense and Micromonospora purpureochromogenes. Fatty acid patterns also supported the division of the strains, and significant differences between the two groups were found in the amounts of iso-15 : 0, iso-16 : 0, iso-16 : 1 and iso-17 : 0. Furthermore, the two groups showed physiological differences which included utilization of arabinose, trehalose, alanine and sucrose and xylan hydrolysis. Finally, DNA–DNA hybridization and ribotyping studies confirmed that each group represents a novel species. Based on the genotypic and phenotypic data, the novel species Micromonospora lupini sp. nov. (type strain Lupac 14NT =DSM 44874T =LMG 24055T) and Micromonospora saelicesensis sp. nov. (type strain Lupac 09T =DSM 44871T =LMG 24056T) are proposed.
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micromonospora coriariae sp nov isolated from Root Nodules of coriaria myrtifolia
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Martha E Trujillo, Lorena Carro, Reiner M Kroppenstedt, Peter Schumann, Eustoquio MartinezmolinaAbstract:An actinomycete strain, NAR01T, was isolated from Root Nodules of a Coriaria plant. The 16S rRNA gene sequence of strain NAR01T showed most similarity to the type strains of Micromonospora endolithica (98.94 %) and Micromonospora chersina (98.4 %). The chemotaxonomic results obtained confirmed the taxonomic position of the isolate within the genus Micromonospora, and revealed differences at the species level. Physiological and biochemical tests showed that strain NAR01T could be clearly distinguished from its closest phylogenetic neighbours, while DNA–DNA hybridization results indicated that the isolate represents a novel species. On the basis of these results, strain NAR01T (=DSM 44875T=LMG 23557T) is proposed as the type strain of the novel species Micromonospora coriariae sp. nov.
Sofie E De Meyer - One of the best experts on this subject based on the ideXlab platform.
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mesorhizobium carmichaelinearum sp nov isolated from carmichaelineae spp Root Nodules
International Journal of Systematic and Evolutionary Microbiology, 2019Co-Authors: Sofie E De Meyer, Euan K James, M. Andrews, Anne WillemsAbstract:Five strains of Gram-stain-negative, rod-shaped bacteria were isolated from Carmichaelia and Montigena Root Nodules. Based on 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium , and to be most closely related to Mesorhizobium jarvisii ATCC 33669T (100–99.6 % sequence similarity), Mesorhizobium huakuii IAM 14158T (99.9–99.6 %), Mesorhizobium japonicum MAFF303099T (99.8–99.6 %) and Mesorhizobium erdmanii USDA 3471T (99.8–99.5 %). Additionally, the strains formed distinct groups based on housekeeping gene analysis and were most closely related to M. jarvisii ATCC 33669T (89.6–89.5 and 97.6–97.3 % sequence similarity for glnII and recA, respectively), M. erdmanii USDA 3471T (94.3–94.0 and 94.9–94.1 %), M. japonicum MAFF303099T (90.0–89.9 and 96.7–96.2 %) and M. huakuii IAM 14158T (89.9–90.0 and 95.4–94.9 %). Chemotaxonomic data supported the assignment of the strains to the genus Mesorhizobium and DNA–DNA hybridizations, average nucleotide identity analysis, matrix-assisted laser desorption ionization time-of-flight MS analysis, physiological and biochemical tests differentiated them genotypically and phenotypically from their nearest neighbouring species. Therefore, these strains are considered to represent a novel species, for which the name Mesorhizobium carmichaelinearum sp. nov. is proposed. The type strain is ICMP 18942T (=MonP1N1T=LMG 28414T).
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mesorhizobium waimense sp nov isolated from sophora longicarinata Root Nodules and mesorhizobium cantuariense sp nov isolated from sophora microphylla Root Nodules
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Sofie E De Meyer, P. B. Heenan, M. Andrews, Anne WillemsAbstract:In total 14 strains of Gram-stain-negative, rod-shaped bacteria were isolated from Sophora longicarinata and Sophora microphylla Root Nodules and authenticated as rhizobia on these hosts. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, and the strains from S. longicarinata were most closely related to Mesorhizobium amorphae ACCC 19665(T) (99.8-99.9 %), Mesorhizobium huakuii IAM 14158(T) (99.8-99.9 %), Mesorhizobium loti USDA 3471(T) (99.5-99.9 %) and Mesorhizobium septentrionale SDW 014(T) (99.6-99.8 %), whilst the strains from S. microphylla were most closely related to Mesorhizobium ciceri UPM-Ca7(T) (99.8-99.9 %), Mesorhizobium qingshengii CCBAU 33460(T) (99.7 %) and Mesorhizobium shangrilense CCBAU 65327(T) (99.6 %). Additionally, these strains formed two distinct groups in phylogenetic trees of the housekeeping genes glnll, recA and rpoB. Chemotaxonomic data, including fatty acid profiles, supported the assignment of the strains to the genus Mesorhizobium and allowed differentiation from the closest neighbours. Results of DNA-DNA hybridizations, MALDI- TOF MS analysis, ERIC-PCR, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their closest neighbouring species. Therefore, the strains isolated from S. longicarinata and S. microphylla represent two novel species for which the names Mesorhizobium waimense sp. nov. (ICMP 19557(T)=LMG 28228(T)=HAMBI 3608(T)) and Mesorhizobium cantuariense sp. nov. (ICMP 19515(T)=LMG 28225(T)=HAMBI 3604(T)), are proposed respectively.
Am Perlick - One of the best experts on this subject based on the ideXlab platform.
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transcriptome profiling in Root Nodules and arbuscular mycorrhiza identifies a collection of novel genes induced during medicago truncatula Root endosymbioses
Molecular Plant-microbe Interactions, 2004Co-Authors: Katja Manthey, Alfred Pühler, Am Perlick, Franziska Krajinski, Natalija Hohnjec, Christian Firnhaber, Helge KusterAbstract:Transcriptome profiling based on cDNA array hybridizations and in silico screening was used to identify Medicago truncatula genes induced in both Root Nodules and arbuscular mycorrhiza (AM). By array hybridizations, we detected several hundred genes that were upregulated in the Root nodule and the AM symbiosis, respectively, with a total of 75 genes being induced during both interactions. The second approach based on in silico data mining yielded several hundred additional candidate genes with a predicted symbiosis-enhanced expression. A subset of the genes identified by either expression profiling tool was subjected to quantitative real-time reverse-transcription polymerase chain reaction for a verification of their symbiosis-induced expression. That way, induction in Root Nodules and AM was confirmed for 26 genes, most of them being reported as symbiosis-induced for the first time. In addition to delivering a number of novel symbiosis-induced genes, our approach identified several genes that were induced in only one of the two Root endosymbioses. The spatial expression patterns of two symbiosis-induced genes encoding an annexin and a beta-tubulin were characterized in transgenic Roots using promoter-reporter gene fusions.
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the medicago truncatula sucrose synthase gene mtsucs1 is activated both in the infected region of Root Nodules and in the cortex of Roots colonized by arbuscular mycorrhizal fungi
Molecular Plant-microbe Interactions, 2003Co-Authors: Natalija Hohnjec, Alfred Pühler, Am Perlick, Helge KusterAbstract:The MtSucS1 gene encodes a sucrose synthase (EC 2.4.1.13) in the model legume Medicago truncatula. To determine the expression pattern of this gene in different organs and in particular during Root endosymbioses, we transformed M. truncatula with specific regions of MtSucS1 fused to the gusAint reporter gene. These fusions directed an induction to the vasculature of leaves, stems, and Roots as well as to flowers, developing seeds, young pods, and germinating seedlings. In Root Nodules, strong promoter activity occurred in the infected cells of the nitrogen-fixing zone but was additionally observed in the meristematic region, the prefixing zone, and the inner cortex, including the vasculature. Concerning endomycorrhizal Roots, the MtSucS1 promoter mediated strongest expression in cortical cells harboring arbuscules. Specifically in highly colonized Root sections, GUS-staining was furthermore detected in the surrounding cortical cells, irrespective of a direct contact with fungal structures. In accordance with the presence of an orthologous PsSus1 gene, we observed a comparable regulation of MtSucS1 expression in the grain legume Pisum sativum in response to microbial symbionts. Unlike other members of the MtSucS gene family, the presence of rhizobial or Glomus microsymbionts significantly altered and enhanced MtSucS1 gene expression, leading us to propose that MtSucS1 is involved in generating sink-strength, not only in Root Nodules but also in mycorrhizal Roots.
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The temporal and spatial transcription pattern in Root Nodules of Vicia faba nodulin genes encoding glycine-rich proteins
'Springer Science and Business Media LLC', 1997Co-Authors: Schroder G, Fruhling M, Pühler Alfred, Am PerlickAbstract:Schroder G, Fruhling M, Pühler A, Perlick AM. The temporal and spatial transcription pattern in Root Nodules of Vicia faba nodulin genes encoding glycine-rich proteins. PLANT MOLECULAR BIOLOGY. 1997;33(1):113-123.Four different transcript sequences encoding gene products with an unusually high glycine content were identified in Vicia faba Root Nodules. Northern blot analysis revealed a strong nodule specific expression of the corresponding genes. Time course experiments showed that two of these genes were transcribed before the onset of leghemoglobin expression and hence were designated VfENOD-GRP2 and VfENOD-GRP5, whereas the first detection of VfNOD-GRP1 and VfNOD-GRP4 transcripts coincided with the appearence of leghemoglobin transcripts in V. faba Root Nodules. A characteristic feature of all encoded nodulins was a hydrophobic N-terminus, which in the case of the nodulins ENOD-GRP2 and ENOD-GRP5 has the characteristics of a signal peptide. Such a structure is comparable to other plant glycine-rich proteins decribed as components of the plant cell wall. Based on tissue print hybridizations, we found that VfNOD-GRP1, VfENOD-GRP2 and VfNOD-GRP4 were expressed in the interzone II-III and in the whole nitrogen-fixing zone III. In contrast to VfENOD-GRP2 and VfNOD-GRP4, the signal intensity of hybridizing VfNOD-GRP1 transcripts was slightly reduced in the more proximal part of broad bean Root Nodules. Apart from the interzone II-III and the nitrogen fixing zone III, VfENOD-GRP5 RNA was also detected in large areas of the prefixing zone II
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the temporal and spatial transcription pattern in Root Nodules of vicia faba nodulin genes encoding glycine rich proteins
Plant Molecular Biology, 1997Co-Authors: Gerald Schroder, Martin Frühling, Alfred Pühler, Am PerlickAbstract:Four different transcript sequences encoding gene products with an unusually high glycine content were identified in Vicia faba Root Nodules. Northern blot analysis revealed a strong nodule specific expression of the corresponding genes. Time course experiments showed that two of these genes were transcribed before the onset of leghemoglobin expression and hence were designated VfENOD-GRP2 and VfENOD-GRP5, whereas the first detection of VfNOD-GRP1 and VfNOD-GRP4 transcripts coincided with the appearance of leghemoglobin transcripts in V. faba Root Nodules. A characteristic feature of all encoded nodulins was a hydrophobic N-terminus, which in the case of the nodulins ENOD-GRP2 and ENOD-GRP5 has the characteristics of a signal peptide. Such a structure is comparable to other plant glycine-rich proteins described as components of the plant cell wall. Based on tissue print hybridizations, we found that VfNOD-GRP1, VfENOD-GRP2 and VfNOD-GRP4 were expressed in the interzone II-III and in the whole nitrogen-fixing zone III. In contrast to VfENOD-GRP2 and VfNOD-GRP4, the signal intensity of hybridizing VfNOD-GRP1 transcripts was slightly reduced in the more proximal part of broad bean Root Nodules. Apart from the interzone II-III and the nitrogen fixing zone III, VfENOD-GRP5 RNA was also detected in large areas of the prefixing zone II.
Carroll P Vance - One of the best experts on this subject based on the ideXlab platform.
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nadh glutamate synthase in alfalfa Root Nodules immunocytochemical localization
Plant Physiology, 1999Co-Authors: Gian Trepp, David W Plank, Stephen J Gantt, Carroll P VanceAbstract:In Root Nodules of alfalfa (Medicago sativa L.), N2 is reduced to NH4+ in the bacteroid by the nitrogenase enzyme and then released into the plant cytosol. The NH4+ is then assimilated by the combined action of glutamine synthetase (EC 6.3.1.2) and NADH-dependent Glu synthase (NADH-GOGAT; EC 1.4.1.14) into glutamine and Glu. The alfalfa nodule NADH-GOGAT protein has a 101-amino acid presequence, but the subcellular location of the protein is unknown. Using immunocytochemical localization, we determined first that the NADH-GOGAT protein is found throughout the infected cell region of both 19- and 33-d-old Nodules. Second, in alfalfa Root Nodules NADH-GOGAT is localized predominantly to the amyloplast of infected cells. This finding, together with earlier localization and fractionation studies, indicates that in alfalfa the infected cells are the main location for the initial assimilation of fixed N2.
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nadh glutamate synthase in alfalfa Root Nodules genetic regulation and cellular expression
Plant Physiology, 1999Co-Authors: Gian Trepp, Stephen J Gantt, Martijn Van De Mortel, Hirofumi Yoshioka, Susan S Miller, Deborah A Samac, Carroll P VanceAbstract:NADH-dependent glutamate synthase (NADH-GOGAT; EC 1.4.1.14) is a key enzyme in primary nitrogen assimilation in alfalfa (Medicago sativa L.) Root Nodules. Here we report that in alfalfa, a single gene, probably with multiple alleles, encodes for NADH-GOGAT. In situ hybridizations were performed to assess the location of NADH-GOGAT transcript in alfalfa Root Nodules. In wild-type cv Saranac Nodules the NADH-GOGAT gene is predominantly expressed in infected cells. Nodules devoid of bacteroids (empty) induced by Sinorhizobium meliloti 7154 had no NADH-GOGAT transcript detectable by in situ hybridization, suggesting that the presence of the bacteroid may be important for NADH-GOGAT expression. The pattern of expression of NADH-GOGAT shifted during Root nodule development. Until d 9 after planting, all infected cells appeared to express NADH-GOGAT. By d 19, a gradient of expression from high in the early symbiotic zone to low in the late symbiotic zone was observed. In 33-d-old Nodules expression was seen in only a few cell layers in the early symbiotic zone. This pattern of expression was also observed for the nifH transcript but not for leghemoglobin. The promoter of NADH-GOGAT was evaluated in transgenic alfalfa plants carrying chimeric β-glucuronidase promoter fusions. The results suggest that there are at least four regulatory elements. The region responsible for expression in the infected cell zone contains an 88-bp direct repeat.
Anne Willems - One of the best experts on this subject based on the ideXlab platform.
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mesorhizobium carmichaelinearum sp nov isolated from carmichaelineae spp Root Nodules
International Journal of Systematic and Evolutionary Microbiology, 2019Co-Authors: Sofie E De Meyer, Euan K James, M. Andrews, Anne WillemsAbstract:Five strains of Gram-stain-negative, rod-shaped bacteria were isolated from Carmichaelia and Montigena Root Nodules. Based on 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium , and to be most closely related to Mesorhizobium jarvisii ATCC 33669T (100–99.6 % sequence similarity), Mesorhizobium huakuii IAM 14158T (99.9–99.6 %), Mesorhizobium japonicum MAFF303099T (99.8–99.6 %) and Mesorhizobium erdmanii USDA 3471T (99.8–99.5 %). Additionally, the strains formed distinct groups based on housekeeping gene analysis and were most closely related to M. jarvisii ATCC 33669T (89.6–89.5 and 97.6–97.3 % sequence similarity for glnII and recA, respectively), M. erdmanii USDA 3471T (94.3–94.0 and 94.9–94.1 %), M. japonicum MAFF303099T (90.0–89.9 and 96.7–96.2 %) and M. huakuii IAM 14158T (89.9–90.0 and 95.4–94.9 %). Chemotaxonomic data supported the assignment of the strains to the genus Mesorhizobium and DNA–DNA hybridizations, average nucleotide identity analysis, matrix-assisted laser desorption ionization time-of-flight MS analysis, physiological and biochemical tests differentiated them genotypically and phenotypically from their nearest neighbouring species. Therefore, these strains are considered to represent a novel species, for which the name Mesorhizobium carmichaelinearum sp. nov. is proposed. The type strain is ICMP 18942T (=MonP1N1T=LMG 28414T).
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mesorhizobium waimense sp nov isolated from sophora longicarinata Root Nodules and mesorhizobium cantuariense sp nov isolated from sophora microphylla Root Nodules
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Sofie E De Meyer, P. B. Heenan, M. Andrews, Anne WillemsAbstract:In total 14 strains of Gram-stain-negative, rod-shaped bacteria were isolated from Sophora longicarinata and Sophora microphylla Root Nodules and authenticated as rhizobia on these hosts. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, and the strains from S. longicarinata were most closely related to Mesorhizobium amorphae ACCC 19665(T) (99.8-99.9 %), Mesorhizobium huakuii IAM 14158(T) (99.8-99.9 %), Mesorhizobium loti USDA 3471(T) (99.5-99.9 %) and Mesorhizobium septentrionale SDW 014(T) (99.6-99.8 %), whilst the strains from S. microphylla were most closely related to Mesorhizobium ciceri UPM-Ca7(T) (99.8-99.9 %), Mesorhizobium qingshengii CCBAU 33460(T) (99.7 %) and Mesorhizobium shangrilense CCBAU 65327(T) (99.6 %). Additionally, these strains formed two distinct groups in phylogenetic trees of the housekeeping genes glnll, recA and rpoB. Chemotaxonomic data, including fatty acid profiles, supported the assignment of the strains to the genus Mesorhizobium and allowed differentiation from the closest neighbours. Results of DNA-DNA hybridizations, MALDI- TOF MS analysis, ERIC-PCR, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their closest neighbouring species. Therefore, the strains isolated from S. longicarinata and S. microphylla represent two novel species for which the names Mesorhizobium waimense sp. nov. (ICMP 19557(T)=LMG 28228(T)=HAMBI 3608(T)) and Mesorhizobium cantuariense sp. nov. (ICMP 19515(T)=LMG 28225(T)=HAMBI 3604(T)), are proposed respectively.
