The Experts below are selected from a list of 1923 Experts worldwide ranked by ideXlab platform
Lasse Saaby - One of the best experts on this subject based on the ideXlab platform.
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triterpene acids from Rose Hip powder inhibit self antigen and lps induced cytokine production and cd4 t cell proliferation in human mononuclear cell cultures
Phytotherapy Research, 2012Co-Authors: Lasse Saaby, Claus Henrik NielsenAbstract:A triterpene acid mixture consisting of oleanolic, ursolic and betulinic acid isolated from a standardized Rose Hip powder (Rosa canina L.) has been shown to inhibit interleukin (IL)-6 release from Mono Mac 6 cells. The present study examined the effects of the triterpene acid mixture on the cytokine production and proliferation of CD4⁺ T cells and CD19⁺ B cells induced by a self-antigen, human thyroglobulin and by lipopolysaccharide in cultures of normal mononuclear cells. The triterpene acid mixture inhibited the production of tumor necrosis factor-α and IL-6 with estimated IC₅₀ values in the range 35-56 µg/mL, the Th1 cytokines interferon-γ and IL-2 (IC₅₀ values 10-20 µg/mL) and the antiinflammatory cytokine IL-10 (IC₅₀ values 18-21 µg/mL). Moreover, the mixture also inhibited CD4⁺ T-cell and CD19⁺ B-cell proliferation (IC₅₀ value 22 and 12 µg/mL, respectively). Together, these data demonstrate that oleanolic, ursolic and betulinic acid are active immunomodulatory constituents of the standardized Rose Hip powder. However, since the estimated IC₅₀ values are in the µg/mL range, it is questionable whether the content of the triterpene acids in the standardized Rose Hip powder, alone, can explain the reported clinical effects.
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Isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder (Rosa canina L.).
Phytotherapy research : PTR, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder rosa canina l
Phytotherapy Research, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.
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Isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder (Rosa canina L.)
Phytotherapy Research, 2010Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previous published systematic review and a metaanalysis have concluded that consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and to mainly involve infiltration of the synovial membrane by macrophages. Therefore, the immunmodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µM). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
Søren Brøgger Christensen - One of the best experts on this subject based on the ideXlab platform.
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Isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder (Rosa canina L.).
Phytotherapy research : PTR, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder rosa canina l
Phytotherapy Research, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.
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Isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder (Rosa canina L.)
Phytotherapy Research, 2010Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previous published systematic review and a metaanalysis have concluded that consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and to mainly involve infiltration of the synovial membrane by macrophages. Therefore, the immunmodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µM). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
Anna K. Jäger - One of the best experts on this subject based on the ideXlab platform.
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Isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder (Rosa canina L.).
Phytotherapy research : PTR, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder rosa canina l
Phytotherapy Research, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.
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Isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder (Rosa canina L.)
Phytotherapy Research, 2010Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previous published systematic review and a metaanalysis have concluded that consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and to mainly involve infiltration of the synovial membrane by macrophages. Therefore, the immunmodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µM). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of linoleic and α linolenic acids as cox 1 and 2 inhibitors in Rose Hip
Phytotherapy Research, 2008Co-Authors: Anna K. Jäger, K. N. Petersen, G. Thomasen, Brogger S ChristensenAbstract:Rose Hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of Rose Hip have showed inhibition of cyclooxygenase-1 and -2. A petroleum ether extract of Rose Hip was fractioned by VLC on silica; on a C-18 column and by HPLC. Each step was COX-1/2 activity-guided. The bioassay-guided fractionation led to the isolation of linoleic acid (the IC50 for COX-1 was 85 µm and 0.6 µm for COX-2) and α-linolenic acid (the IC50 for COX-1 was 52 µm and 12 µm for COX-2). The COX-2/COX-1 ratio was 0.007 for linoleic acid and 0.2 for α-linolenic acid. Linoleic acid and α-linolenic acid contribute to the COX-1 and -2 inhibitory activity of Rose Hip. Copyright © 2008 John Wiley & Sons, Ltd.
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Isolation of linoleic and α‐linolenic acids as COX‐1 and ‐2 inhibitors in Rose Hip
Phytotherapy research : PTR, 2008Co-Authors: Anna K. Jäger, K. N. Petersen, G. Thomasen, S. Brøgger ChristensenAbstract:Rose Hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of Rose Hip have showed inhibition of cyclooxygenase-1 and -2. A petroleum ether extract of Rose Hip was fractioned by VLC on silica; on a C-18 column and by HPLC. Each step was COX-1/2 activity-guided. The bioassay-guided fractionation led to the isolation of linoleic acid (the IC50 for COX-1 was 85 µm and 0.6 µm for COX-2) and α-linolenic acid (the IC50 for COX-1 was 52 µm and 12 µm for COX-2). The COX-2/COX-1 ratio was 0.007 for linoleic acid and 0.2 for α-linolenic acid. Linoleic acid and α-linolenic acid contribute to the COX-1 and -2 inhibitory activity of Rose Hip. Copyright © 2008 John Wiley & Sons, Ltd.
K. Winther - One of the best experts on this subject based on the ideXlab platform.
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the effectiveness of a standardized Rose Hip powder containing seeds and shells of rosa canina on cell longevity skin wrinkles moisture and elasticity
Clinical Interventions in Aging, 2015Co-Authors: L Phetcharat, K Wongsuphasawat, K. WintherAbstract:Objective To evaluate the effects of a Rose Hip powder (Hyben Vital®) made from seeds and shells on cell senescence, skin wrinkling, and aging.
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a powder made from seeds and shells of a Rose Hip subspecies rosa canina reduces symptoms of knee and Hip osteoarthritis a randomized double blind placebo controlled clinical trial
Scandinavian Journal of Rheumatology, 2005Co-Authors: K. Winther, K Apel, G ThamsborgAbstract:Objective: The aim of this study was to determine whether a herbal remedy made from a subspecies of Rose‐Hip (Rosa canina) might reduce symptoms of osteoarthritis and consumption of rescue medication in patients suffering from osteoarthritis.Methods: Ninety‐four patients with osteoarthritis of the Hip or knee were enrolled in a randomized, placebo‐controlled, double‐blind crossover trial. Forty‐seven patients were given 5 g of the herbal remedy daily for a period of 3 months and the remaining patients were given a similar amount of placebo. The group initially treated with placebo was then changed to Rose‐Hip and vice versa for another 3‐month period. Upon inclusion and after 3 weeks and 3 months of each treatment period, pain, stiffness, disability, and global severity of the disease were scored on a Western Ontario and McMaster Universities (WOMAC) questionnaire. After 3 weeks of treatment, patients, if possible, were allowed to reduce their consumption of ‘rescue medication’. Data were analysed on the ...
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The anti-inflammatory properties of Rose-Hip
InflammoPharmacology, 1999Co-Authors: K. Winther, E. Rein, A. KharazmiAbstract:-The anti-inflammatory properties of Rose-Hip are described in this short report. Rose-Hip extract reduced chemotaxis of peripheral blood neutrophils and monocytes of healthy subjects in vitro. Daily intake of Rose-Hip powder for four weeks by healthy volunteers and patients suffering from osteoarthritis, resulted in reduced serum C-reactive protein (CRP) levels and reduced chemotaxis of peripheral blood neutrophils. The results indicate that Rose-Hip possesses anti-inflammatory properties and might be used as a replacement or supplement for conventional drug therapies in patients with osteoarthritis.
Erik Wind Hansen - One of the best experts on this subject based on the ideXlab platform.
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Isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder (Rosa canina L.).
Phytotherapy research : PTR, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder rosa canina l
Phytotherapy Research, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.
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Isolation of immunomodulatory triterpene acids from a standardized Rose Hip powder (Rosa canina L.)
Phytotherapy Research, 2010Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previous published systematic review and a metaanalysis have concluded that consumption of standardized Rose Hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and to mainly involve infiltration of the synovial membrane by macrophages. Therefore, the immunmodulatory effect of standardized Rose Hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of Rose Hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µM). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
