The Experts below are selected from a list of 297 Experts worldwide ranked by ideXlab platform
Yinxin Li - One of the best experts on this subject based on the ideXlab platform.
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application potential of Salicornia europaea in remediation of cd pb and li contaminated saline soil
Chinese Journal of Biotechnology, 2020Co-Authors: Sulian Lu, Yinxin LiAbstract:: Coastal and inland saline-alkali soil is important reserve land resources. However, some parts of saline land are now under the threat of heavy metals such as cadmium (Cd), lead (Pb) and the light metal lithium (Li). Phytoremediation with halophytes could be the most economical and effective way to restore the contaminated saline soil. In this study, the growth, physiological and biochemical indexes and ion contents of halophyte Salicornia europaea under different concentrations of Cd (0-50 mmol/L), Pb (0-50 mmol/L) and Li (0-400 mmol/L) were investigated to evaluate the tolerance and accumulation of the metal contaminations. The results showed that plant height, fresh weight and dry weight of S. europaea decreased significantly with the increase of Cd and Pb concentration. Low concentration of Li ( 20 mmol/L). The tolerance order of S. europaea to Cd, Pb and Li was Li > Pb > Cd. Cd, Pb and Li stresses may negatively affected Na and K uptake and transport in S. europaea to affect plant growth. In addition, the antioxidant enzyme system synergistically responsed to resist the oxidative toxicity of different ions. The contents of Cd, Pb, Li in roots and shoots of S. europaea also increased with the increase of treatment concentration. Furthermore, Cd and Pb contents in roots were significantly higher than in shoots, while more Li accumulated in shoots than in roots. The aforementioned results showed that S. europaea had strong tolerance along with a high accumulate ability to Cd, Pb and Li, indicating its application potential in restoring Cd, Pb and Li contaminated saline soil. This study laid a basis for further exploration of the tolerance mechanism of S. europaea to Cd, Pb and Li stresses, and gave a new perspective for the usage of S. europaea to remediate Cd, Pb and Li pollutants in high-salinity alkali soils.
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The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea
Planta, 2017Co-Authors: Sulian Lv, Fang Tai, Duoliya Wang, Pengxiang Fan, Hexigeduleng Bao, Juanjuan Feng, Ping Jiang, Yinxin LiAbstract:Main conclusionThe V-ATPase subunit A participates in vacuolar Na+ compartmentalization inSalicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
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the v atpase subunit a is essential for salt tolerance through participating in vacuolar na compartmentalization in Salicornia europaea
Planta, 2017Co-Authors: Sulian Lv, Duoliya Wang, Juanjuan Feng, Ping Jiang, Yinxin LiAbstract:Main conclusion The V-ATPase subunit A participates in vacuolar Na + compartmentalization in Salicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
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establishment of a gene function analysis system for the euhalophyte Salicornia europaea l
Plant Cell Reports, 2017Co-Authors: Sulian Lv, Juanjuan Feng, Ping Jiang, Jinhui Wang, Yinxin LiAbstract:Key message A Salicornia europaea L. in vitro cell transformation system was developed and further applied to SeNHX1 function investigation.
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H(+)-pyrophosphatase from Salicornia europaea enhances tolerance to low phosphate under salinity in Arabidopsis.
Plant Signaling & Behavior, 2016Co-Authors: Sulian Lv, Duoliya Wang, Ping Jiang, Yinxin LiAbstract:Increasing soil salinity threatens crop productivity worldwide. High soil salinity is usually accompanied by the low availability of many mineral nutrients. Here, we investigated the potential role that the H+- PPase could play in optimizing P use efficiency under salinity in plants. Transgenic Arabidopsis plants overexpressing either SeVP1 or SeVP2 from Salicornia europaea outperformed the wild-types under low phosphate (Pi) as well as low Pi plus salt conditions. Our results suggested that H+-PPase could increase external Pi acquisition through promoting root development and upregulating phosphate transporters, thus to protect plants from Pi limiting stress. This study provides a potential strategy for improving crop yields challenged by the co-occurrence of abiotic stresses.
Sulian Lv - One of the best experts on this subject based on the ideXlab platform.
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The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea
Planta, 2017Co-Authors: Sulian Lv, Fang Tai, Duoliya Wang, Pengxiang Fan, Hexigeduleng Bao, Juanjuan Feng, Ping Jiang, Yinxin LiAbstract:Main conclusionThe V-ATPase subunit A participates in vacuolar Na+ compartmentalization inSalicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
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the v atpase subunit a is essential for salt tolerance through participating in vacuolar na compartmentalization in Salicornia europaea
Planta, 2017Co-Authors: Sulian Lv, Duoliya Wang, Juanjuan Feng, Ping Jiang, Yinxin LiAbstract:Main conclusion The V-ATPase subunit A participates in vacuolar Na + compartmentalization in Salicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
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establishment of a gene function analysis system for the euhalophyte Salicornia europaea l
Plant Cell Reports, 2017Co-Authors: Sulian Lv, Juanjuan Feng, Ping Jiang, Jinhui Wang, Yinxin LiAbstract:Key message A Salicornia europaea L. in vitro cell transformation system was developed and further applied to SeNHX1 function investigation.
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H(+)-pyrophosphatase from Salicornia europaea enhances tolerance to low phosphate under salinity in Arabidopsis.
Plant Signaling & Behavior, 2016Co-Authors: Sulian Lv, Duoliya Wang, Ping Jiang, Yinxin LiAbstract:Increasing soil salinity threatens crop productivity worldwide. High soil salinity is usually accompanied by the low availability of many mineral nutrients. Here, we investigated the potential role that the H+- PPase could play in optimizing P use efficiency under salinity in plants. Transgenic Arabidopsis plants overexpressing either SeVP1 or SeVP2 from Salicornia europaea outperformed the wild-types under low phosphate (Pi) as well as low Pi plus salt conditions. Our results suggested that H+-PPase could increase external Pi acquisition through promoting root development and upregulating phosphate transporters, thus to protect plants from Pi limiting stress. This study provides a potential strategy for improving crop yields challenged by the co-occurrence of abiotic stresses.
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h pyrophosphatase from Salicornia europaea enhances tolerance to low phosphate under salinity in arabidopsis
Plant Signaling & Behavior, 2016Co-Authors: Sulian Lv, Duoliya Wang, Ping Jiang, Yinxin LiAbstract:Increasing soil salinity threatens crop productivity worldwide. High soil salinity is usually accompanied by the low availability of many mineral nutrients. Here, we investigated the potential role that the H+- PPase could play in optimizing P use efficiency under salinity in plants. Transgenic Arabidopsis plants overexpressing either SeVP1 or SeVP2 from Salicornia europaea outperformed the wild-types under low phosphate (Pi) as well as low Pi plus salt conditions. Our results suggested that H+-PPase could increase external Pi acquisition through promoting root development and upregulating phosphate transporters, thus to protect plants from Pi limiting stress. This study provides a potential strategy for improving crop yields challenged by the co-occurrence of abiotic stresses.
Ping Jiang - One of the best experts on this subject based on the ideXlab platform.
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The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea
Planta, 2017Co-Authors: Sulian Lv, Fang Tai, Duoliya Wang, Pengxiang Fan, Hexigeduleng Bao, Juanjuan Feng, Ping Jiang, Yinxin LiAbstract:Main conclusionThe V-ATPase subunit A participates in vacuolar Na+ compartmentalization inSalicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
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the v atpase subunit a is essential for salt tolerance through participating in vacuolar na compartmentalization in Salicornia europaea
Planta, 2017Co-Authors: Sulian Lv, Duoliya Wang, Juanjuan Feng, Ping Jiang, Yinxin LiAbstract:Main conclusion The V-ATPase subunit A participates in vacuolar Na + compartmentalization in Salicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
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establishment of a gene function analysis system for the euhalophyte Salicornia europaea l
Plant Cell Reports, 2017Co-Authors: Sulian Lv, Juanjuan Feng, Ping Jiang, Jinhui Wang, Yinxin LiAbstract:Key message A Salicornia europaea L. in vitro cell transformation system was developed and further applied to SeNHX1 function investigation.
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H(+)-pyrophosphatase from Salicornia europaea enhances tolerance to low phosphate under salinity in Arabidopsis.
Plant Signaling & Behavior, 2016Co-Authors: Sulian Lv, Duoliya Wang, Ping Jiang, Yinxin LiAbstract:Increasing soil salinity threatens crop productivity worldwide. High soil salinity is usually accompanied by the low availability of many mineral nutrients. Here, we investigated the potential role that the H+- PPase could play in optimizing P use efficiency under salinity in plants. Transgenic Arabidopsis plants overexpressing either SeVP1 or SeVP2 from Salicornia europaea outperformed the wild-types under low phosphate (Pi) as well as low Pi plus salt conditions. Our results suggested that H+-PPase could increase external Pi acquisition through promoting root development and upregulating phosphate transporters, thus to protect plants from Pi limiting stress. This study provides a potential strategy for improving crop yields challenged by the co-occurrence of abiotic stresses.
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h pyrophosphatase from Salicornia europaea enhances tolerance to low phosphate under salinity in arabidopsis
Plant Signaling & Behavior, 2016Co-Authors: Sulian Lv, Duoliya Wang, Ping Jiang, Yinxin LiAbstract:Increasing soil salinity threatens crop productivity worldwide. High soil salinity is usually accompanied by the low availability of many mineral nutrients. Here, we investigated the potential role that the H+- PPase could play in optimizing P use efficiency under salinity in plants. Transgenic Arabidopsis plants overexpressing either SeVP1 or SeVP2 from Salicornia europaea outperformed the wild-types under low phosphate (Pi) as well as low Pi plus salt conditions. Our results suggested that H+-PPase could increase external Pi acquisition through promoting root development and upregulating phosphate transporters, thus to protect plants from Pi limiting stress. This study provides a potential strategy for improving crop yields challenged by the co-occurrence of abiotic stresses.
Juanjuan Feng - One of the best experts on this subject based on the ideXlab platform.
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The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea
Planta, 2017Co-Authors: Sulian Lv, Fang Tai, Duoliya Wang, Pengxiang Fan, Hexigeduleng Bao, Juanjuan Feng, Ping Jiang, Yinxin LiAbstract:Main conclusionThe V-ATPase subunit A participates in vacuolar Na+ compartmentalization inSalicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
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the v atpase subunit a is essential for salt tolerance through participating in vacuolar na compartmentalization in Salicornia europaea
Planta, 2017Co-Authors: Sulian Lv, Duoliya Wang, Juanjuan Feng, Ping Jiang, Yinxin LiAbstract:Main conclusion The V-ATPase subunit A participates in vacuolar Na + compartmentalization in Salicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
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establishment of a gene function analysis system for the euhalophyte Salicornia europaea l
Plant Cell Reports, 2017Co-Authors: Sulian Lv, Juanjuan Feng, Ping Jiang, Jinhui Wang, Yinxin LiAbstract:Key message A Salicornia europaea L. in vitro cell transformation system was developed and further applied to SeNHX1 function investigation.
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h pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in arabidopsis and wheat
Plant Cell and Environment, 2015Co-Authors: Sulian Lv, Duoliya Wang, Juanjuan Feng, Ping Jiang, Jinhui Wang, Xianyang Chen, Yinxin LiAbstract:: High salinity and nitrogen (N) deficiency in soil are two key factors limiting crop productivity, and they usually occur simultaneously. Here we firstly found that H(+) -PPase is involved in salt-stimulated NO3 (-) uptake in the euhalophyte Salicornia europaea. Then, two genes (named SeVP1 and SeVP2) encoding H(+) -PPase from S. europaea were characterized. The expression of SeVP1 and SeVP2 was induced by salt stress and N starvation. Both SeVP1 or SeVP2 transgenic Arabidopsis and wheat plants outperformed the wild types (WTs) when high salt and low N occur simultaneously. The transgenic Arabidopsis plants maintained higher K(+) /Na(+) ratio in leaves and exhibited increased NO3 (-) uptake, inorganic pyrophosphate-dependent vacuolar nitrate efflux and assimilation capacity under this double stresses. Furthermore, they had more soluble sugars in shoots and roots and less starch accumulation in shoots than WT. These performances can be explained by the up-regulated expression of ion, nitrate and sugar transporter genes in transgenic plants. Taken together, our results suggest that up-regulation of H(+) -PPase favours the transport of photosynthates to root, which could promote root growth and integrate N and carbon metabolism in plant. This work provides potential strategies for improving crop yields challenged by increasing soil salinization and shrinking farmland.
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high throughput deep sequencing reveals that micrornas play important roles in salt tolerance of euhalophyte Salicornia europaea
BMC Plant Biology, 2015Co-Authors: Juanjuan Feng, Sulian Lv, Ping Jiang, Jinhui Wang, Xianyang Chen, Sandra Chang, Shizhong Li, Yinxin LiAbstract:microRNAs (miRNAs) are implicated in plant development processes and play pivotal roles in plant adaptation to environmental stresses. Salicornia europaea, a salt mash euhalophyte, is a suitable model plant to study salt adaptation mechanisms. S. europaea is also a vegetable, forage, and oilseed that can be used for saline land reclamation and biofuel precursor production on marginal lands. Despite its importance, no miRNA has been identified from S. europaea thus far. Deep sequencing was performed to investigate small RNA transcriptome of S. europaea. Two hundred and ten conserved miRNAs comprising 51 families and 31 novel miRNAs (including seven miRNA star sequences) belonging to 30 families were identified. About half (13 out of 31) of the novel miRNAs were only detected in salt-treated samples. The expression of 43 conserved and 13 novel miRNAs significantly changed in response to salinity. In addition, 53 conserved and 13 novel miRNAs were differentially expressed between the shoots and roots. Furthermore, 306 and 195 S. europaea unigenes were predicted to be targets of 41 conserved and 29 novel miRNA families, respectively. These targets encoded a wide range of proteins, and genes involved in transcription regulation constituted the largest category. Four of these genes encoding laccase, F-box family protein, SAC3/GANP family protein, and NADPH cytochrome P-450 reductase were validated using 5′-RACE. Our results indicate that specific miRNAs are tightly regulated by salinity in the shoots and/or roots of S. europaea, which may play important roles in salt tolerance of this euhalophyte. The S. europaea salt-responsive miRNAs and miRNAs that target transcription factors, nucleotide binding site-leucine-rich repeat proteins and enzymes involved in lignin biosynthesis as well as carbon and nitrogen metabolism may be applied in genetic engineering of crops with high stress tolerance, and genetic modification of biofuel crops with high biomass and regulatable lignin biosynthesis.
Xianyang Chen - One of the best experts on this subject based on the ideXlab platform.
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h pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in arabidopsis and wheat
Plant Cell and Environment, 2015Co-Authors: Sulian Lv, Duoliya Wang, Juanjuan Feng, Ping Jiang, Jinhui Wang, Xianyang Chen, Yinxin LiAbstract:: High salinity and nitrogen (N) deficiency in soil are two key factors limiting crop productivity, and they usually occur simultaneously. Here we firstly found that H(+) -PPase is involved in salt-stimulated NO3 (-) uptake in the euhalophyte Salicornia europaea. Then, two genes (named SeVP1 and SeVP2) encoding H(+) -PPase from S. europaea were characterized. The expression of SeVP1 and SeVP2 was induced by salt stress and N starvation. Both SeVP1 or SeVP2 transgenic Arabidopsis and wheat plants outperformed the wild types (WTs) when high salt and low N occur simultaneously. The transgenic Arabidopsis plants maintained higher K(+) /Na(+) ratio in leaves and exhibited increased NO3 (-) uptake, inorganic pyrophosphate-dependent vacuolar nitrate efflux and assimilation capacity under this double stresses. Furthermore, they had more soluble sugars in shoots and roots and less starch accumulation in shoots than WT. These performances can be explained by the up-regulated expression of ion, nitrate and sugar transporter genes in transgenic plants. Taken together, our results suggest that up-regulation of H(+) -PPase favours the transport of photosynthates to root, which could promote root growth and integrate N and carbon metabolism in plant. This work provides potential strategies for improving crop yields challenged by increasing soil salinization and shrinking farmland.
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high throughput deep sequencing reveals that micrornas play important roles in salt tolerance of euhalophyte Salicornia europaea
BMC Plant Biology, 2015Co-Authors: Juanjuan Feng, Sulian Lv, Ping Jiang, Jinhui Wang, Xianyang Chen, Sandra Chang, Shizhong Li, Yinxin LiAbstract:microRNAs (miRNAs) are implicated in plant development processes and play pivotal roles in plant adaptation to environmental stresses. Salicornia europaea, a salt mash euhalophyte, is a suitable model plant to study salt adaptation mechanisms. S. europaea is also a vegetable, forage, and oilseed that can be used for saline land reclamation and biofuel precursor production on marginal lands. Despite its importance, no miRNA has been identified from S. europaea thus far. Deep sequencing was performed to investigate small RNA transcriptome of S. europaea. Two hundred and ten conserved miRNAs comprising 51 families and 31 novel miRNAs (including seven miRNA star sequences) belonging to 30 families were identified. About half (13 out of 31) of the novel miRNAs were only detected in salt-treated samples. The expression of 43 conserved and 13 novel miRNAs significantly changed in response to salinity. In addition, 53 conserved and 13 novel miRNAs were differentially expressed between the shoots and roots. Furthermore, 306 and 195 S. europaea unigenes were predicted to be targets of 41 conserved and 29 novel miRNA families, respectively. These targets encoded a wide range of proteins, and genes involved in transcription regulation constituted the largest category. Four of these genes encoding laccase, F-box family protein, SAC3/GANP family protein, and NADPH cytochrome P-450 reductase were validated using 5′-RACE. Our results indicate that specific miRNAs are tightly regulated by salinity in the shoots and/or roots of S. europaea, which may play important roles in salt tolerance of this euhalophyte. The S. europaea salt-responsive miRNAs and miRNAs that target transcription factors, nucleotide binding site-leucine-rich repeat proteins and enzymes involved in lignin biosynthesis as well as carbon and nitrogen metabolism may be applied in genetic engineering of crops with high stress tolerance, and genetic modification of biofuel crops with high biomass and regulatable lignin biosynthesis.
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sodium plays a more important role than potassium and chloride in growth of Salicornia europaea
Acta Physiologiae Plantarum, 2012Co-Authors: Sulian Lv, Xianyang Chen, Xuchu Wang, Dan Jiang, Yinxin LiAbstract:Salicornia europaea is a succulent euhalophyte that belongs to the Chenopodiaceae family. It is found that moderate concentration of NaCl can dramatically stimulate the growth of S. europaea plants. To elucidate the mech- anism underlying the phenomenon, morphological and physiological changes of S. europaea in response to dif- ferent ions, including cations (Na ? ,K ? ,L i ? ,C s ? ) and anions (Cl - ,N O 3 - ,C H 3COO - ) were investigated, and the effects of Na ? ,C l - and K ? on the growth of S. europaea were also studied. Na ? was more effective than K ? and Cl - in stimulating shoot succulence, cell expansion, and stomatal opening. Plants treated with Na ? (including NaCl, Na ? , NaNO3) showed better plant growth, increased pho- tosynthesis and less cell membrane damage than those untreated and treated with 200 mM of Cl - and K ? (including KCl and KNO3). Both SEM-X-Ray microanal- ysis and flame emission results revealed that well devel- oped S. europaea plants had a higher content of sodium but lower potassium and chlorine. It is concluded that sodium plays a more important role in the growth and development of S. europaea than potassium and chloride.
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multiple compartmentalization of sodium conferred salt tolerance in Salicornia europaea
Plant Physiology and Biochemistry, 2012Co-Authors: Sulian Lv, Ping Jiang, Xianyang Chen, Xuchu Wang, Yinxin LiAbstract:Euhalophyte Salicornia europaea L., one of the most salt-tolerant plant species in the world, can tolerate more than 1000 mM NaCl. To study the salt tolerance mechanism of this plant, the effects of different NaCl concentrations on plant growth, as well as Na þ accumulation and distribution at organ, tissue, and subcellular levels, were investigated. Optimal growth and an improved photosynthetic rate were observed with the plant treated with 200e400 mM NaCl. The Na þ content in the shoots was considerably higher than that in the roots of S. europaea. The Na þ in S. europaea cells may act as an effective osmotic adjuster to maintain cell turgor, promoting photosynthetic competence and plant growth. The results from the SEMeX-ray and TEMeX-ray microanalyses demonstrate that Na þ was compartmentalized predominantly into the cell vacuoles of shoot endodermis tissues. Accordingly, the transcript amounts of SeNHX1, SeVHA-A, and SeVP1 increased significantly with increased NaCl concentration, suggesting their important roles in Na þ sequestration into the vacuoles. Therefore, a multiple sodium compartmentali
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coordination of carbon fixation and nitrogen metabolism in Salicornia europaea under salinity comparative proteomic analysis on chloroplast proteins
Proteomics, 2011Co-Authors: Juanjuan Feng, Sulian Lv, Ping Jiang, Xianyang Chen, Dan Jiang, Tingyun Kuang, Yinxin LiAbstract:: Halophyte, like Salicornia europaea, could make full use of marginal saline land for carbon fixation. How the photosynthesis of S. europaea is regulated under high salinity implicates a significant aspect to exploit this pioneer plant in future. Measurement of photosynthesis parameters demonstrated the reduction of photosynthesis for the 0 and 800 mM NaCl treated plants are more likely due to non-stomatal limitation, which might be caused by changes in the enzymes associated with photosynthesis. Different salinity induced ultrastructure changes other than photosynthetic apparatus damage, suggesting the photosynthesis of S. europaea might be affected via biochemical regulation. Comparative proteomics analysis of chloroplast proteins by 2-D gel electrophoresis reproducibly detected 90 differentially expressed proteins, among which 66 proteins were identified by nanoLC MS/MS. Further study of thylakoid membrane proteins by Blue-Native PAGE proved the increase in abundance of light reaction proteins under salinity. Analysis of gene expression patterns of 12 selected proteins provides evidence for the correlations between transcription and proteomics data. Based on our results, a putative model of photosynthesis regulatory network figured out proper coordination of carbon fixation and nitrogen metabolism in chloroplast of S. europaea under salinity, which provided subcellular level insight into salt tolerance mechanism in S. europaea.
