Salmonella Agona

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J. F. Frank - One of the best experts on this subject based on the ideXlab platform.

  • Relative survival of four serotypes of Salmonella enterica in low-water activity whey protein powder held at 36 and 70°C at various water activity levels.
    Journal of Food Protection, 2014
    Co-Authors: Sofia M. Santillana Farakos, J. W. Hicks, J.g. Frye, J. F. Frank
    Abstract:

    Salmonella enterica is not able to grow at water activity (aw) levels below 0.94, but it can survive in low-aw foods for long periods of time. Temperature, aw, substrate, and serotype affect its persistence. The aim of this study was to evaluate the influence of temperature and aw on the relative persistence among four serotypes of Salmonella enterica in low-aw whey protein powder. Whey protein powder was equilibrated to aws 0.18 ± 0.02 and 0.54 ± 0.03, inoculated with a cocktail of Salmonella serovars (Agona, Tennessee, Montevideo, and Typhimurium), vacuum sealed, and stored at 36°C for 6 months and at 70°C for 48 h. Presumptive Salmonella colonies (30 to 32) were randomly picked from each plate at the end of each survival study. PCR multiplex serotyping was used to identify the isolates. A multinomial mixed logistic model with Salmonella Tennessee as a reference was used to test for significant differences in frequency distribution of the surviving serotypes. Salmonella Tennessee and Salmonella Agona we...

  • Relative Survival of Four Serotypes of Salmonella enterica in Low–Water Activity Whey Protein Powder Held at 36 and 70°C at Various Water Activity Levels
    Journal of Food Protection, 2014
    Co-Authors: S. M. Santillana Farakos, J. W. Hicks, J.g. Frye, J. F. Frank
    Abstract:

    Salmonella enterica is not able to grow at water activity (aw) levels below 0.94, but it can survive in low-aw foods for long periods of time. Temperature, aw, substrate, and serotype affect its persistence. The aim of this study was to evaluate the influence of temperature and aw on the relative persistence among four serotypes of Salmonella enterica in low-aw whey protein powder. Whey protein powder was equilibrated to aws 0.18 ± 0.02 and 0.54 ± 0.03, inoculated with a cocktail of Salmonella serovars (Agona, Tennessee, Montevideo, and Typhimurium), vacuum sealed, and stored at 36°C for 6 months and at 70°C for 48 h. Presumptive Salmonella colonies (30 to 32) were randomly picked from each plate at the end of each survival study. PCR multiplex serotyping was used to identify the isolates. A multinomial mixed logistic model with Salmonella Tennessee as a reference was used to test for significant differences in frequency distribution of the surviving serotypes. Salmonella Tennessee and Salmonella Agona were the most prevalent surviving serotypes, followed in decreasing order by Salmonella Montevideo and Salmonella Typhimurium. Statistical analysis indicated that temperature (P = 0.003) and aw (P = 0.012) influenced the relative prevalence of the Salmonella serotypes. If other environmental conditions are equal, Salmonella Tennessee is better able to survive than Salmonella Montevideo and Salmonella Typhimurium at higher temperatures and higher aw levels in low-aw whey protein powder held at 36 and 70°C. The relative prevalence of Salmonella Agona to Salmonella Tennessee did not change with increasing temperature (P = 0.211) or aw (P = 0.453). These results should be considered in risk assessment and when developing predictive models for survival of Salmonella in low-aw foods.

Charlene R Jackson - One of the best experts on this subject based on the ideXlab platform.

  • characterization of Salmonella enterica serovar Agona slaughter isolates from the animal arm of the national antimicrobial resistance monitoring system enteric bacteria narms 1997 through 2003
    Microbial Drug Resistance, 2008
    Co-Authors: Aphrodite Douris, Paula J Fedorkacray, Charlene R Jackson
    Abstract:

    A total of 499 Salmonella enterica serovar Agona isolates from cattle, swine, chicken, and turkey samples were assayed for antimicrobial susceptibility and subtyped using pulsed-field gel electrophoresis (PFGE). Salmonella Agona isolates exhibited increased resistance to amoxicillin/clavulanic acid, ampicillin, cefoxitin, ceftiofur, cephalothin, and chloramphenicol, and a single isolate was resistant to ceftriaxone. Multiple drug resistance (MDR; resistance ≥ 2 antimicrobials) was exhibited in 57% (n = 282/499) of the Salmonella Agona isolates and 22% (n = 111/499) of these Salmonella Agona isolates were resistant to five or more antimicrobials. PFGE patterns of 482 Salmonella Agona slaughter samples resulted in 165 unique patterns. Cluster analysis indicated that isolates indistinguishable by PFGE appeared to group according to antimicrobial resistance profiles. These data suggest that Salmonella Agona is increasing in prevalence in U.S. cattle presented for slaughter and should be further monitored.

N Nicolay - One of the best experts on this subject based on the ideXlab platform.

  • Salmonella enterica serovar Agona european outbreak associated with a food company
    Epidemiology and Infection, 2011
    Co-Authors: N Nicolay, Martin Cormican, P Mckeown, Lelia Thornton, S Cotter, P Garvey, O Bannon, I S T Fisher, C L Little, N S Boxall
    Abstract:

    : We investigated an international outbreak of Salmonella Agona with a distinct PFGE pattern associated with an Irish Food company (company X) producing pre-cooked meat products sold in various food outlet chains in Europe. The outbreak was first detected in Ireland. We undertook national and international case-finding, food traceback and microbiological investigation of human, food and environmental samples. We undertook a matched case-control study on Irish cases. In total, 163 cases in seven European countries were laboratory-confirmed. Consumption of food from food outlet chains supplied by company X was significantly associated with being a confirmed case (mOR 18·3, 95% CI 2·2-149·2) in the case-control study. The outbreak strain was isolated from the company's pre-cooked meat products and production premises. Sufficient evidence was gathered to infer the vehicles of infection and sources of the outbreak and to justify the control measures taken, which were plant closure and food recall.

  • a multi country outbreak of Salmonella Agona february august 2008
    Eurosurveillance, 2008
    Co-Authors: Darina Oflanagan, Martin Cormican, P Mckeown, N Nicolay, J Cowden, Brendan W Mason, Dilys Morgan, C Lane, N Irvine, L Browning
    Abstract:

    An outbreak of gastroenteritis affecting residents in the United Kingdom, Ireland and Finland is currently being investigated. As of Wednesday 13 August, a total of 119 cases have been identified. An investigation that includes interviews of persons with Salmonella Agona infections, comparison of pulsed field gel electrophoresis (PFGE) profiles of S. Agona isolates from cases and also food samples from an Irish food production company and retail outlet chain supplied by the company, suggests that food products from that company may be related to some of these cases. A number of food products including beef steak strips, chicken in various forms, bacon in various forms, and pork have been withdrawn (see: http://www.fsai.ie/ for details). The investigation is ongoing.

Henriette De Valk - One of the best experts on this subject based on the ideXlab platform.

N S Boxall - One of the best experts on this subject based on the ideXlab platform.

  • Salmonella enterica serovar Agona european outbreak associated with a food company
    Epidemiology and Infection, 2011
    Co-Authors: N Nicolay, Martin Cormican, P Mckeown, Lelia Thornton, S Cotter, P Garvey, O Bannon, I S T Fisher, C L Little, N S Boxall
    Abstract:

    : We investigated an international outbreak of Salmonella Agona with a distinct PFGE pattern associated with an Irish Food company (company X) producing pre-cooked meat products sold in various food outlet chains in Europe. The outbreak was first detected in Ireland. We undertook national and international case-finding, food traceback and microbiological investigation of human, food and environmental samples. We undertook a matched case-control study on Irish cases. In total, 163 cases in seven European countries were laboratory-confirmed. Consumption of food from food outlet chains supplied by company X was significantly associated with being a confirmed case (mOR 18·3, 95% CI 2·2-149·2) in the case-control study. The outbreak strain was isolated from the company's pre-cooked meat products and production premises. Sufficient evidence was gathered to infer the vehicles of infection and sources of the outbreak and to justify the control measures taken, which were plant closure and food recall.