The Experts below are selected from a list of 38280 Experts worldwide ranked by ideXlab platform
Xin Zhao - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Using In Vitro Immunomodulatory Properties of Lactic Acid Bacteria for Selection of Probiotics against Salmonella Infection in Broiler Chicks
2016Co-Authors: Junchang Feng, Luoxiong Zhou, Xin Yang, Xin ZhaoAbstract:Poultry is known to be a major reservoir of Salmonella. The use of lactic acid bacteria has become one of successful strategies to control Salmonella in poultry. The purpose of this study was to select lactic acid bacteria strains by their in vitro immunomodulatory properties for potential use as probiotics against Salmonella Infection in broiler chicks. Among 101 iso-lated lactic acid bacteria strains, 13 strains effectively survived under acidic (pH 2.5) and bile salt (ranging from 0.1 % to 1.0%) conditions, effectively inhibited growth of 6 pathogens, and adhered to Caco-2 cells. However, their in vitro immunomodulatory activities differed significantly. Finally, three strains with higher in vitro immunomodulatory properties (Lacto-bacillus plantarum PZ01, Lactobacillus salivarius JM32 and Pediococcus acidilactici JH231) and three strains with lower in vitro immunomodulatory activities (Enterococcus fae-cium JS11, Lactobacillus salivarius JK22 and Lactobacillus salivarius JM2A1) were com-pared for their inhibitory effects on Salmonella adhesion and invasion to Caco-2 cells in vitro and their antimicrobial effects in vivo. The former three strains inhibited Salmonella adhe-sion and invasion to Caco-2 cells in vitro, reduced the number of Salmonella in intestinal content, spleen and liver, reduced the levels of lipopolysaccharide-induced TNF-α factor (LITAF), IL-1β, IL-6 and IL-12 in serum and increased the level of IL-10 in serum during a challenge study in vivomore efficiently than the latter three strains. These results suggest that in vitro immunomodulatory activities could be used as additional parameters to select more effective probiotics as feed supplements for poultry
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using in vitro immunomodulatory properties of lactic acid bacteria for selection of probiotics against Salmonella Infection in broiler chicks
PLOS ONE, 2016Co-Authors: Junchang Feng, Lihong Wang, Luoxiong Zhou, Xin Yang, Xin ZhaoAbstract:Poultry is known to be a major reservoir of Salmonella. The use of lactic acid bacteria has become one of successful strategies to control Salmonella in poultry. The purpose of this study was to select lactic acid bacteria strains by their in vitro immunomodulatory properties for potential use as probiotics against Salmonella Infection in broiler chicks. Among 101 isolated lactic acid bacteria strains, 13 strains effectively survived under acidic (pH 2.5) and bile salt (ranging from 0.1% to 1.0%) conditions, effectively inhibited growth of 6 pathogens, and adhered to Caco-2 cells. However, their in vitro immunomodulatory activities differed significantly. Finally, three strains with higher in vitro immunomodulatory properties (Lactobacillus plantarum PZ01, Lactobacillus salivarius JM32 and Pediococcus acidilactici JH231) and three strains with lower in vitro immunomodulatory activities (Enterococcus faecium JS11, Lactobacillus salivarius JK22 and Lactobacillus salivarius JM2A1) were compared for their inhibitory effects on Salmonella adhesion and invasion to Caco-2 cells in vitro and their antimicrobial effects in vivo. The former three strains inhibited Salmonella adhesion and invasion to Caco-2 cells in vitro, reduced the number of Salmonella in intestinal content, spleen and liver, reduced the levels of lipopolysaccharide-induced TNF-α factor (LITAF), IL-1β, IL-6 and IL-12 in serum and increased the level of IL-10 in serum during a challenge study in vivo more efficiently than the latter three strains. These results suggest that in vitro immunomodulatory activities could be used as additional parameters to select more effective probiotics as feed supplements for poultry.
Christopher A Lopez - One of the best experts on this subject based on the ideXlab platform.
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endogenous enterobacteriaceae underlie variation in susceptibility to Salmonella Infection
Nature microbiology, 2019Co-Authors: Eric M Velazquez, Henry Nguyen, Keaton T Heasley, Cheng Saechao, Lindsey M Gil, Andrew W L Rogers, Brittany M Miller, Matthew Rolston, Christopher A LopezAbstract:Lack of reproducibility is a prominent problem in biomedical research. An important source of variation in animal experiments is the microbiome, but little is known about specific changes in the microbiota composition that cause phenotypic differences. Here, we show that genetically similar laboratory mice obtained from four different commercial vendors exhibited marked phenotypic variation in their susceptibility to Salmonella Infection. Faecal microbiota transplant into germ-free mice replicated donor susceptibility, revealing that variability was due to changes in the gut microbiota composition. Co-housing of mice only partially transferred protection against Salmonella Infection, suggesting that minority species within the gut microbiota might confer this trait. Consistent with this idea, we identified endogenous Enterobacteriaceae, a low-abundance taxon, as a keystone species responsible for variation in the susceptibility to Salmonella Infection. Protection conferred by endogenous Enterobacteriaceae could be modelled by inoculating mice with probiotic Escherichia coli, which conferred resistance by using its aerobic metabolism to compete with Salmonella for resources. We conclude that a mechanistic understanding of phenotypic variation can accelerate development of strategies for enhancing the reproducibility of animal experiments. Variable susceptibility to Salmonella Infection across genetically similar mice from commercial vendors is due to differential colonization of the gut microbiome by endogenous Enterobacteriaceae.
Jakub Voznica - One of the best experts on this subject based on the ideXlab platform.
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identification of parameters of host cell vulnerability during Salmonella Infection by quantitative image analysis and modeling
Infection and Immunity, 2017Co-Authors: Jakub Voznica, Christophe Gardella, Ilia Belotserkovsky, Alexandre Dufour, Jost Enninga, Virginie SteveninAbstract:ABSTRACT Salmonella targets and enters epithelial cells at permissive entry sites: some cells are more likely to be infected than others. However, the parameters that lead to host cell heterogeneity are not known. Here, we quantitatively characterized host cell vulnerability to Salmonella Infection based on imaged parameters. We performed successive Infections of the same host cell population followed by automated high-throughput microscopy and observed that infected cells have a higher probability of being reinfected. Establishing a predictive model, we identified two combined origins of host cell vulnerability: pathogen-induced cellular vulnerability emerging from Salmonella uptake and persisting at later stages of the Infection and host cell-inherent vulnerability. We linked the host cell-inherent vulnerability with its morphological attributes, such as local cell crowding, and with host cell cholesterol content. This showed that the probability of Salmonella Infection success can be forecast from morphological or molecular host cell parameters.
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identifying parameters of host cell vulnerability during Salmonella Infection by quantitative image analysis and modeling
bioRxiv, 2017Co-Authors: Jakub Voznica, Christophe Gardella, Ilia Belotserkovsky, Alexandre Dufour, Jost Enninga, Virginie SteveninAbstract:Salmonella target and enter epithelial cells at permissive entry sites: some cells are more likely to be infected than others. However the parameters that lead to host cell heterogeneity are not known. Here, we quantitatively characterized host cell ‘vulnerability’ towards Salmonella Infection based on imaged parameters. We performed successive Infections of the same host cell population followed by automated high-throuput microscopy and observed that infected cells have higher probability of being re-infected. Establishing a predictive model we identified two combined origins of host cell vulnerability: the pathogen-induced cellular vulnerability emerging from Salmonella uptake and persisting at later stage of the Infection, and the host cell-inherent vulnerability. We linked the host cell inherent vulnerability with its morphological attributes such as the local cell crowding, and with host cell cholesterol content. This showed that the probability of Salmonella Infection success can be forecast from morphological or molecular host cell parameters.
Stephen J. Mcsorley - One of the best experts on this subject based on the ideXlab platform.
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tlr5 deficient mice lack basal inflammatory and metabolic defects but exhibit impaired cd4 t cell responses to a flagellated pathogen
Journal of Immunology, 2011Co-Authors: Shirdi Letran, Seungjoo Lee, Shaikh M Atif, Adriana Floreslangarica, Satoshi Uematsu, Shizuo Akira, Adam F Cunningham, Stephen J. McsorleyAbstract:TLR5-deficient mice have been reported to develop spontaneous intestinal inflammation and metabolic abnormalities. However, we report that TLR5-deficient mice from two different animal colonies display no evidence of basal inflammatory disease, metabolic abnormalities, or enhanced resistance to Salmonella Infection. In contrast, the absence of TLR5 hindered the initial activation and clonal expansion of intestinal flagellin-specific CD4 T cells following oral Salmonella Infection. Together, these data demonstrate that a basal inflammatory phenotype is not a consistent feature of TLR5-deficient mice and document a novel role for TLR5 in the rapid targeting of flagellin by intestinal pathogen-specific CD4 T cells.
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expression of toll il 1r domain containing adaptor protein tirap is detrimental to primary clearance of Salmonella and is not required for the generation of protective immunity
Immunology Letters, 2008Co-Authors: Stu Jerke, Aparna Srinivasan, Stephen J. McsorleyAbstract:Salmonella Infection triggers activation of innate immune cells through the interaction of bacterial products with Toll-like receptors (TLRs). Toll/IL-1R domain-containing adaptor protein (TIRAP) is an adaptor protein involved in downstream signaling through TLRs 1, 2, 4, and 6. We examined the role of TIRAP during Infection with attenuated Salmonella. Surprisingly, TIRAP-deficient mice were fully capable of resolving primary Infection with Salmonella and actually exhibited accelerated clearance of bacteria at a late stage of the Infection. Consistent with enhanced bacterial clearance, TIRAP-deficient mice resolved bacterial-associated splenic inflammation more rapidly than wild-type (Wt) mice and splenocytes from Salmonella-infected TIRAP-deficient mice produced more IFN-γ upon in vitro re-stimulation. Upon secondary challenge, TIRAP-deficient and Wt mice displayed a similar level of protective immunity against virulent Salmonella. Together these data indicate that TIRAP-mediated signaling is completely dispensable for clearance of Salmonella Infection, and actually has a mild deleterious effect upon the resolution of primary Infection.
Luis Morera - One of the best experts on this subject based on the ideXlab platform.
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Early Salmonella Typhimurium Infection in pigs disrupts Microbiome composition and functionality principally at the ileum mucosa
Scientific Reports, 2018Co-Authors: Héctor Argüello, Jordi Jordi Estellé, Sara Zaldívar-lópez, Ana Carvajal, Mª Asunción López-bascón, Fiona Crispie, Orla O’sullivan, Paul D. Cotter, Feliciano Priego-capote, Luis MoreraAbstract:Salmonella is a major foodborne pathogen which successfully infects animal species for human consumption such as swine. The pathogen has a battery of virulence factors which it uses to colonise and persist within the host. The host microbiota may play a role in resistance to, and may also be indirectly responsible from some of the consequences of, Salmonella Infection. To investigate this, we used 16S rRNA metagenomic sequencing to determine the changes in the gut microbiota of pigs in response to Infection by Salmonella Typhimurium at three locations: ileum mucosa, ileum content and faeces. Early Infection (2 days post-Infection) impacted on the microbiome diversity at the mucosa, reflected in a decrease in representatives of the generally regarded as desirable genera (i.e., Bifidobacterium and Lactobacillus). Severe damage in the epithelium of the ileum mucosa correlated with an increase in synergistic (with respect to Salmonella Infection; Akkermansia) or opportunistically pathogenic bacteria (Citrobacter) and a depletion in anaerobic bacteria (Clostridium spp., Ruminococcus, or Dialliser). Predictive functional analysis, together with metabolomic analysis revealed changes in glucose and lipid metabolism in infected pigs. The observed changes in commensal healthy microbiota, including the growth of synergistic or potentially pathogenic bacteria and depletion of beneficial or competing bacteria, could contribute to the pathogen's ability to colonize the gut successfully. The findings from this study could be used to form the basis for further research aimed at creating intervention strategies to mitigate the effects of Salmonella Infection.
