Sambucus nigra

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Els J. M. Van Damme - One of the best experts on this subject based on the ideXlab platform.

  • A complex fruit‐specific type‐2 ribosome‐inactivating protein from elderberry (Sambucus nigra) is correctly processed and assembled in transgenic tobacco plants
    FEBS Journal, 2002
    Co-Authors: Ying Chen, Pierre Rouge, Willy J Peumans, Frank Vandenbussche, Paul Proost, Els J. M. Van Damme
    Abstract:

    Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers. A cDNA clone encoding this so-called Sambucus nigra fruit specific agglutinin I (SNA-If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA-If from tobacco leaves has the same molecular structure and biological activities as native SNA-If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type-2 ribosome-inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA-If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type-2 RIP SNA-If does not act as an antiviral agent in planta.

  • a complex fruit specific type 2 ribosome inactivating protein from elderberry Sambucus nigra is correctly processed and assembled in transgenic tobacco plants
    FEBS Journal, 2002
    Co-Authors: Ying Chen, Pierre Rouge, Willy J Peumans, Frank Vandenbussche, Paul Proost, Els J. M. Van Damme
    Abstract:

    Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers. A cDNA clone encoding this so-called Sambucus nigra fruit specific agglutinin I (SNA-If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA-If from tobacco leaves has the same molecular structure and biological activities as native SNA-If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type-2 ribosome-inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA-If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type-2 RIP SNA-If does not act as an antiviral agent in planta.

  • the Sambucus nigra type 2 ribosome inactivating protein sna i exhibits in planta antiviral activity in transgenic tobacco
    FEBS Letters, 2002
    Co-Authors: Ying Chen, Willy J Peumans, Els J. M. Van Damme
    Abstract:

    Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA-IP from Sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. Expression of SNA-IP under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type-1 ribosome-inactivating proteins, the expression of SNA-IP does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis-related proteins in- dicating that its antiviral activity most probably differs from that of pokeweed antiviral protein. fl 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

  • The Sambucus nigra type-2 ribosome-inactivating protein SNA-I' exhibits in planta antiviral activity in transgenic tobacco.
    FEBS letters, 2002
    Co-Authors: Ying Chen, Willy J Peumans, Els J. M. Van Damme
    Abstract:

    Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA-I' from Sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. Expression of SNA-I' under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type-1 ribosome-inactivating proteins, the expression of SNA-I' does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis-related proteins indicating that its antiviral activity most probably differs from that of pokeweed antiviral protein.

  • Preparation of monospecific polyclonal antibodies against Sambucus nigra lectin related protein, a glycosylated plant protein.
    Preparative Biochemistry & Biotechnology, 2001
    Co-Authors: Stijn Desmyter, Frank Vandenbussche, Els J. M. Van Damme, Willy J Peumans
    Abstract:

    A simple, but highly efficient, method was developed for the purification of monospecific antibodies against the plant glycoprotein Sambucus nigra lectin related protein. In a first step, the antiserum is purified by affinity chromatography on a column with the immobilized antigen. To deplete the affinity-purified antiserum from aspecific cross-reacting antibodies directed against the glycan part of the glycoprotein, a second affinity chromatography on an unrelated plant glycoprotein, in casu the Robinia pseudoacacia agglutinin, is included.

Ying Chen - One of the best experts on this subject based on the ideXlab platform.

  • a complex fruit specific type 2 ribosome inactivating protein from elderberry Sambucus nigra is correctly processed and assembled in transgenic tobacco plants
    FEBS Journal, 2002
    Co-Authors: Ying Chen, Pierre Rouge, Willy J Peumans, Frank Vandenbussche, Paul Proost, Els J. M. Van Damme
    Abstract:

    Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers. A cDNA clone encoding this so-called Sambucus nigra fruit specific agglutinin I (SNA-If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA-If from tobacco leaves has the same molecular structure and biological activities as native SNA-If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type-2 ribosome-inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA-If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type-2 RIP SNA-If does not act as an antiviral agent in planta.

  • A complex fruit‐specific type‐2 ribosome‐inactivating protein from elderberry (Sambucus nigra) is correctly processed and assembled in transgenic tobacco plants
    FEBS Journal, 2002
    Co-Authors: Ying Chen, Pierre Rouge, Willy J Peumans, Frank Vandenbussche, Paul Proost, Els J. M. Van Damme
    Abstract:

    Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers. A cDNA clone encoding this so-called Sambucus nigra fruit specific agglutinin I (SNA-If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA-If from tobacco leaves has the same molecular structure and biological activities as native SNA-If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type-2 ribosome-inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA-If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type-2 RIP SNA-If does not act as an antiviral agent in planta.

  • the Sambucus nigra type 2 ribosome inactivating protein sna i exhibits in planta antiviral activity in transgenic tobacco
    FEBS Letters, 2002
    Co-Authors: Ying Chen, Willy J Peumans, Els J. M. Van Damme
    Abstract:

    Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA-IP from Sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. Expression of SNA-IP under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type-1 ribosome-inactivating proteins, the expression of SNA-IP does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis-related proteins in- dicating that its antiviral activity most probably differs from that of pokeweed antiviral protein. fl 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

  • The Sambucus nigra type-2 ribosome-inactivating protein SNA-I' exhibits in planta antiviral activity in transgenic tobacco.
    FEBS letters, 2002
    Co-Authors: Ying Chen, Willy J Peumans, Els J. M. Van Damme
    Abstract:

    Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA-I' from Sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. Expression of SNA-I' under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type-1 ribosome-inactivating proteins, the expression of SNA-I' does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis-related proteins indicating that its antiviral activity most probably differs from that of pokeweed antiviral protein.

Willy J Peumans - One of the best experts on this subject based on the ideXlab platform.

  • a complex fruit specific type 2 ribosome inactivating protein from elderberry Sambucus nigra is correctly processed and assembled in transgenic tobacco plants
    FEBS Journal, 2002
    Co-Authors: Ying Chen, Pierre Rouge, Willy J Peumans, Frank Vandenbussche, Paul Proost, Els J. M. Van Damme
    Abstract:

    Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers. A cDNA clone encoding this so-called Sambucus nigra fruit specific agglutinin I (SNA-If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA-If from tobacco leaves has the same molecular structure and biological activities as native SNA-If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type-2 ribosome-inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA-If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type-2 RIP SNA-If does not act as an antiviral agent in planta.

  • A complex fruit‐specific type‐2 ribosome‐inactivating protein from elderberry (Sambucus nigra) is correctly processed and assembled in transgenic tobacco plants
    FEBS Journal, 2002
    Co-Authors: Ying Chen, Pierre Rouge, Willy J Peumans, Frank Vandenbussche, Paul Proost, Els J. M. Van Damme
    Abstract:

    Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers. A cDNA clone encoding this so-called Sambucus nigra fruit specific agglutinin I (SNA-If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA-If from tobacco leaves has the same molecular structure and biological activities as native SNA-If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type-2 ribosome-inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA-If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type-2 RIP SNA-If does not act as an antiviral agent in planta.

  • the Sambucus nigra type 2 ribosome inactivating protein sna i exhibits in planta antiviral activity in transgenic tobacco
    FEBS Letters, 2002
    Co-Authors: Ying Chen, Willy J Peumans, Els J. M. Van Damme
    Abstract:

    Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA-IP from Sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. Expression of SNA-IP under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type-1 ribosome-inactivating proteins, the expression of SNA-IP does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis-related proteins in- dicating that its antiviral activity most probably differs from that of pokeweed antiviral protein. fl 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

  • The Sambucus nigra type-2 ribosome-inactivating protein SNA-I' exhibits in planta antiviral activity in transgenic tobacco.
    FEBS letters, 2002
    Co-Authors: Ying Chen, Willy J Peumans, Els J. M. Van Damme
    Abstract:

    Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA-I' from Sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. Expression of SNA-I' under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type-1 ribosome-inactivating proteins, the expression of SNA-I' does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis-related proteins indicating that its antiviral activity most probably differs from that of pokeweed antiviral protein.

  • Preparation of monospecific polyclonal antibodies against Sambucus nigra lectin related protein, a glycosylated plant protein.
    Preparative Biochemistry & Biotechnology, 2001
    Co-Authors: Stijn Desmyter, Frank Vandenbussche, Els J. M. Van Damme, Willy J Peumans
    Abstract:

    A simple, but highly efficient, method was developed for the purification of monospecific antibodies against the plant glycoprotein Sambucus nigra lectin related protein. In a first step, the antiserum is purified by affinity chromatography on a column with the immobilized antigen. To deplete the affinity-purified antiserum from aspecific cross-reacting antibodies directed against the glycan part of the glycoprotein, a second affinity chromatography on an unrelated plant glycoprotein, in casu the Robinia pseudoacacia agglutinin, is included.

Frederic Mortier - One of the best experts on this subject based on the ideXlab platform.

Pierre Rouge - One of the best experts on this subject based on the ideXlab platform.

  • A complex fruit‐specific type‐2 ribosome‐inactivating protein from elderberry (Sambucus nigra) is correctly processed and assembled in transgenic tobacco plants
    FEBS Journal, 2002
    Co-Authors: Ying Chen, Pierre Rouge, Willy J Peumans, Frank Vandenbussche, Paul Proost, Els J. M. Van Damme
    Abstract:

    Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers. A cDNA clone encoding this so-called Sambucus nigra fruit specific agglutinin I (SNA-If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA-If from tobacco leaves has the same molecular structure and biological activities as native SNA-If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type-2 ribosome-inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA-If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type-2 RIP SNA-If does not act as an antiviral agent in planta.

  • a complex fruit specific type 2 ribosome inactivating protein from elderberry Sambucus nigra is correctly processed and assembled in transgenic tobacco plants
    FEBS Journal, 2002
    Co-Authors: Ying Chen, Pierre Rouge, Willy J Peumans, Frank Vandenbussche, Paul Proost, Els J. M. Van Damme
    Abstract:

    Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers. A cDNA clone encoding this so-called Sambucus nigra fruit specific agglutinin I (SNA-If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA-If from tobacco leaves has the same molecular structure and biological activities as native SNA-If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type-2 ribosome-inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA-If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type-2 RIP SNA-If does not act as an antiviral agent in planta.

  • the major elderberry Sambucus nigra fruit protein is a lectin derived from a truncated type 2 ribosome inactivating protein
    Plant Journal, 1997
    Co-Authors: E J M Van Damme, Annick Barre, Pierre Rouge, F Van Leuven, Willy J Peumans
    Abstract:

    The major protein of elderberry (Sambucus nigra L.) fruits is a lectin, called Sambucus nigra agglutinin IVf or SNAIVf. This lectin is composed of subunits that strongly resemble the B chain of the type 2 ribosome-inactivating protein (RIP), called SNAVf, present in the same tissue. To corroborate the possible relationship between both proteins their corresponding cDNAs were cloned and compared. Alignment of the deduced amino acid sequences revealed that the cDNA encoding SNAIVf is almost identical to that of SNAVf except that its A chain is truncated. Northern blot analysis confirmed that the mRNA encoding SNAIVf is about 500 nucleotides shorter than the SNAVf mRNA. In addition, the occurrence of a truncated type 2 RIP gene was unambiguously demonstrated by the analysis of PCR amplified genomic sequences. These results not only demonstrate for the first time that a plant lectin is encoded by a truncated type 2 RIP gene but also address important questions with respect to the molecular evolution of RIP and lectins.

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