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Nakayo Cabada, Luis Alberto - One of the best experts on this subject based on the ideXlab platform.
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Influencia de los residuos de construcción y demolición (RCD) en la resistencia a la compresión y succión del ladrillo de concreto artesanal – Trujillo 2018 (Tesis parcial)
'Dipartimento di Economia Universita di Perugia (IT)', 2019Co-Authors: Chávez Ruíz, Ericsson Alen, Nakayo Cabada, Luis AlbertoAbstract:ABSTRACT In this thesis, the influence of recycled concrete material (RCD) was investigated as result of the demolition of sidewalks, which was reused to replace part of the coarse aggregate for the preparation of the mixture and its application in the manufacture of concrete bricks. Artisanal. The RCD was obtained by the traditional process of crushing, the resulting from the demolition of paths to obtain a Maximum size nominal TMN of ¾ ", which was mixed in proportions of 5%, 10%, 20%, 30%, 40%, 50%, 60% and 75%, with the coarse aggregate, to obtain a semi-dry mixture with a settlement of 0-2 ", characterization of all the materials, and with a mix design for each combination. The results showed that RCD in proportions of 5% and 10% did not significantly influence the Sample Pattern, which had a design of F'c = 100kg/cm2 in the resistance to compression, as well as the percentage of suction Obtained for these proportions was less than 20 g/200cm2/min. By making an assessment, these Samples fluctuated in the range of 116 kg/cm2, 127 kg/cm2 and 125 kg/cm2 respectively, locating our brick unit in a type III according to its classification according to standard E. 070. It was noted that as the proportion of RCD increased, the resistance was declining, and the dispersion was increasing. In the economic aspect, the RCD can cost 28.00 soles per cubic meter, cheaper than the 3/4 "gravel with a cost of 35.00 soles per m3, to date a RCD crushing plant would have to be implemented, which reduce the price of this input. In the environmental issue, the recycling of this material will help mitigate in part the over-exploitation of quarries. It will lead to fostering the care of the environment by generating an appropriate use of construction and demolition waste, which we see every day as part of the landscape of our city.TesisRESUMEN En la presente tesis se investigó la influencia de material reciclado del concreto (RCD) como resultado de la demolición de veredas, que se utilizó en reemplazo de parte del agregado grueso para la elaboración de la mezcla y su aplicación en la fabricación de ladrillos de concreto artesanal. El RCD se obtuvo por proceso de chancado en forma artesanal del desmonte, producto de la demolición de veredas para obtener un TMN de ¾”, el cual se mezcló en proporciones de 0%, 5%, 10%, 20%, 30%, 40%, 50%, 60% y 75% con el agregado grueso, para la obtención de una mezcla semi seca con un asentamiento de 0-2”, la caracterización de todos los materiales, y con un diseño de mezclas para cada combinación. En los resultados obtenidos se evidenció, que el RCD en proporciones de 5% y 10%, no influyen significativamente con respecto a la muestra patrón, la cual tenía un diseño de f’c =100kg/cm2 en la resistencia a la compresión, así como también el porcentaje de succión que se obtuvo para estas proporciones fue menor a 20 g/200cm2/min. Haciendo una valoración, estas muestras fluctuaban en el rango de 116 kg/cm2, 127 kg/cm2 y 125 kg/cm2 respectivamente, ubicando a nuestra unidad de ladrillo en un TIPO III de acuerdo con su clasificación según la Norma E.070. Se llegó a constatar que a medida que aumentaba la proporción de RCD la resistencia iba disminuyendo y la dispersión iba aumentando. En el aspecto económico el RCD puede llegar a costar 28.00 soles el m3, más barato que la gravilla de 3/4” con un costo de 35.00 soles el m3, a la fecha se tendría que implementar una planta de chancado de RCD, la cual reduciría el precio de este insumo. En el tema ambiental, el reciclaje de este material ayudará a mitigar en parte la sobre explotación de canteras. Conllevará a favorecer el cuidado del medio ambiente, al generar un uso apropiado de los residuos de construcción y demolición, que vemos a diario formando parte del paisaje de nuestra ciudad
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Influencia de los residuos de construcción y demolición RCD en la resistencia a la compresión y succión del ladrillo de concreto artesanal – Trujillo 2018
Universidad Privada del Norte, 2019Co-Authors: Chávez Ruíz, Ericsson Alen, Nakayo Cabada, Luis AlbertoAbstract:RESUMEN En la presente tesis se investigó la influencia de material reciclado del concreto (RCD) como resultado de la demolición de veredas, que se utilizó en reemplazo de parte del agregado grueso para la elaboración de la mezcla y su aplicación en la fabricación de ladrillos de concreto artesanal. El RCD se obtuvo por proceso de chancado en forma artesanal del desmonte, producto de la demolición de veredas para obtener un TMN de ¾”, el cual se mezcló en proporciones de 0%, 5%, 10%, 20%, 30%, 40%, 50%, 60% y 75% con el agregado grueso, para la obtención de una mezcla semi seca con un asentamiento de 0-2”, la caracterización de todos los materiales, y con un diseño de mezclas para cada combinación. En los resultados obtenidos se evidenció, que el RCD en proporciones de 5% y 10%, no influyen significativamente con respecto a la muestra patrón, la cual tenía un diseño de f’c =100kg/cm2 en la resistencia a la compresión, así como también el porcentaje de succión que se obtuvo para estas proporciones fue menor a 20 g/200cm2/min. Haciendo una valoración, estas muestras fluctuaban en el rango de 116 kg/cm2, 127 kg/cm2 y 125 kg/cm2 respectivamente, ubicando a nuestra unidad de ladrillo en un TIPO III de acuerdo con su clasificación según la Norma E.070. Se llegó a constatar que a medida que aumentaba la proporción de RCD la resistencia iba disminuyendo y la dispersión iba aumentando. En el aspecto económico el RCD puede llegar a costar 28.00 soles el m3, más barato que la gravilla de 3/4” con un costo de 35.00 soles el m3, a la fecha se tendría que implementar una planta de chancado de RCD, la cual reduciría el precio de este insumo. En el tema ambiental, el reciclaje de este material ayudará a mitigar en parte la sobre explotación de canteras. Conllevará a favorecer el cuidado del medio ambiente, al generar un uso apropiado de los residuos de construcción y demolición, que vemos a diario formando parte del paisaje de nuestra ciudad. PALABRAS CLAVES: RCD, proporción de RCD, resistencia a la compresión, succión, agregados, ladrillos de concreto artesanal.ABSTRACT In this thesis, the influence of recycled concrete material (RCD) was investigated as result of the demolition of sidewalks, which was reused to replace part of the coarse aggregate for the preparation of the mixture and its application in the manufacture of concrete bricks. Artisanal. The RCD was obtained by the traditional process of crushing, the resulting from the demolition of paths to obtain a Maximum size nominal TMN of ¾ ", which was mixed in proportions of 5%, 10%, 20%, 30%, 40%, 50%, 60% and 75%, with the coarse aggregate, to obtain a semi-dry mixture with a settlement of 0-2 ", characterization of all the materials, and with a mix design for each combination. The results showed that RCD in proportions of 5% and 10% did not significantly influence the Sample Pattern, which had a design of F'c = 100kg/cm2 in the resistance to compression, as well as the percentage of suction Obtained for these proportions was less than 20 g/200cm2/min. By making an assessment, these Samples fluctuated in the range of 116 kg/cm2, 127 kg/cm2 and 125 kg/cm2 respectively, locating our brick unit in a type III according to its classification according to standard E. 070. It was noted that as the proportion of RCD increased, the resistance was declining, and the dispersion was increasing. In the economic aspect, the RCD can cost 28.00 soles per cubic meter, cheaper than the 3/4 "gravel with a cost of 35.00 soles per m3, to date a RCD crushing plant would have to be implemented, which reduce the price of this input. In the environmental issue, the recycling of this material will help mitigate in part the over-exploitation of quarries. It will lead to fostering the care of the environment by generating an appropriate use of construction and demolition waste, which we see every day as part of the landscape of our city. KEYWORDS: RCD, RCD proportion, compression resistance, suction, aggregates, craft concrete bricks
Wolfram Weckwerth - One of the best experts on this subject based on the ideXlab platform.
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integration of metabolomics and proteomics in molecular plant physiology coping with the complexity by data dimensionality reduction
Physiologia Plantarum, 2008Co-Authors: Wolfram WeckwerthAbstract:In recent years, genomics has been extended to functional genomics. Toward the characterization of organisms or species on the genome level, changes on the metabolite and protein level have been shown to be essential to assign functions to genes and to describe the dynamic molecular phenotype. Gas chromatography (GC) and liquid chromatography coupled to mass spectrometry (GC- and LC-MS) are well suited for the fast and comprehensive analysis of ultracomplex metabolite Samples. For the integration of metabolite profiles with quantitative protein profiles, a high throughput (HTP) shotgun proteomics approach using LC-MS and label-free quantification of unique proteins in a complex protein digest is described. Multivariate statistics are applied to examine Sample Pattern recognition based on data-dimensionality reduction and biomarker identification in plant systems biology. The integration of the data reveal multiple correlative biomarkers providing evidence for an increase of information in such holistic approaches. With computational simulation of metabolic networks and experimental measurements, it can be shown that biochemical regulation is reflected by metabolite network dynamics measured in a metabolomics approach. Examples in molecular plant physiology are presented to substantiate the integrative approach.
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Integration of metabolomic and proteomic phenotypes: analysis of data covariance dissects starch and RFO metabolism from low and high temperature compensation response in Arabidopsis thaliana.
Molecular & cellular proteomics : MCP, 2008Co-Authors: Stefanie Wienkoop, Katja Morgenthal, Florian Wolschin, Matthias Scholz, Joachim Selbig, Wolfram WeckwerthAbstract:Statistical mining and integration of complex molecular data including metabolites, proteins, and transcripts is one of the critical goals of systems biology (Ideker, T., Galitski, T., and Hood, L. (2001) A new approach to decoding life: systems biology. Annu. Rev. Genomics Hum. Genet. 2, 343-372). A number of studies have demonstrated the parallel analysis of metabolites and large scale transcript expression. Protein analysis has been ignored in these studies, although a clear correlation between transcript and protein levels is shown only in rare cases, necessitating that actual protein levels have to be determined for protein function analysis. Here, we present an approach to investigate the combined covariance structure of metabolite and protein dynamics in a systemic response to abiotic temperature stress in Arabidopsis thaliana wild-type and a corresponding starch-deficient mutant (phosphoglucomutase-deficient). Independent component analysis revealed phenotype classification resolving genotype-dependent response effects to temperature treatment and genotype-independent general temperature compensation mechanisms. An observation is the stress-induced increase of raffinose-family-oligosaccharide levels in the absence of transitory starch storage/mobilization in temperature-treated phosphoglucomutase plants indicating that sucrose synthesis and storage in these mutant plants is sufficient to bypass the typical starch storage/mobilization pathways under abiotic stress. Eventually, Sample Pattern recognition and correlation network topology analysis allowed for the detection of specific metabolite-protein co-regulation and assignment of a circadian output regulated RNA-binding protein to these processes. The whole concept of high-dimensional profiling data integration from many replicates, subsequent multivariate statistics for dimensionality reduction, and covariance structure analysis is proposed to be a major strategy for revealing central responses of the biological system under study.
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stable isotope free quantitative shotgun proteomics combined with Sample Pattern recognition for rapid diagnostics
Journal of Separation Science, 2006Co-Authors: Stefanie Wienkoop, Estibaliz Larrainzar, Michaela Niemann, Esther M Gonzalez, Ute Lehmann, Wolfram WeckwerthAbstract:Mass spectrometry (MS) has become a powerful tool for the quantitative analysis of complex protein Samples. A high-throughput strategy for the comparative analysis of multiple protein Samples with high complexity becomes more and more important. Two strategies, spectral count and peak intensity, for label-free MS analysis of prefractionated complex mixtures have been described recently to be useful for quantitation. Here we compare both strategies for rapid and quantitative 1-D shotgun LC/MS/MS analyses of highly complex protein mixtures using silica-based monolithic columns. First, we validated linearity and sensitivity of these methods by spiking varying amounts of an internal standard protein in a complex plant protein extract. Secondly, quantitative data of proteins of Medicago truncatula nodules were visualized with independent components analysis using data either obtained from spectral count or peak integration performed with commercial software. Spectral count showed apparent advantages over peak integration because several peptides per protein are automatically averaged, the linear dynamic range of quantitation increases in complex matrices and the number of quantified proteins surpasses the number of proteins using peak integration. Thus, for the need of rapid comparative analysis of highly complex protein Samples, spectral count enables Sample Pattern recognition and identification of biomarkers in nongel based proteomic studies.
Chávez Ruíz, Ericsson Alen - One of the best experts on this subject based on the ideXlab platform.
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Influencia de los residuos de construcción y demolición (RCD) en la resistencia a la compresión y succión del ladrillo de concreto artesanal – Trujillo 2018 (Tesis parcial)
'Dipartimento di Economia Universita di Perugia (IT)', 2019Co-Authors: Chávez Ruíz, Ericsson Alen, Nakayo Cabada, Luis AlbertoAbstract:ABSTRACT In this thesis, the influence of recycled concrete material (RCD) was investigated as result of the demolition of sidewalks, which was reused to replace part of the coarse aggregate for the preparation of the mixture and its application in the manufacture of concrete bricks. Artisanal. The RCD was obtained by the traditional process of crushing, the resulting from the demolition of paths to obtain a Maximum size nominal TMN of ¾ ", which was mixed in proportions of 5%, 10%, 20%, 30%, 40%, 50%, 60% and 75%, with the coarse aggregate, to obtain a semi-dry mixture with a settlement of 0-2 ", characterization of all the materials, and with a mix design for each combination. The results showed that RCD in proportions of 5% and 10% did not significantly influence the Sample Pattern, which had a design of F'c = 100kg/cm2 in the resistance to compression, as well as the percentage of suction Obtained for these proportions was less than 20 g/200cm2/min. By making an assessment, these Samples fluctuated in the range of 116 kg/cm2, 127 kg/cm2 and 125 kg/cm2 respectively, locating our brick unit in a type III according to its classification according to standard E. 070. It was noted that as the proportion of RCD increased, the resistance was declining, and the dispersion was increasing. In the economic aspect, the RCD can cost 28.00 soles per cubic meter, cheaper than the 3/4 "gravel with a cost of 35.00 soles per m3, to date a RCD crushing plant would have to be implemented, which reduce the price of this input. In the environmental issue, the recycling of this material will help mitigate in part the over-exploitation of quarries. It will lead to fostering the care of the environment by generating an appropriate use of construction and demolition waste, which we see every day as part of the landscape of our city.TesisRESUMEN En la presente tesis se investigó la influencia de material reciclado del concreto (RCD) como resultado de la demolición de veredas, que se utilizó en reemplazo de parte del agregado grueso para la elaboración de la mezcla y su aplicación en la fabricación de ladrillos de concreto artesanal. El RCD se obtuvo por proceso de chancado en forma artesanal del desmonte, producto de la demolición de veredas para obtener un TMN de ¾”, el cual se mezcló en proporciones de 0%, 5%, 10%, 20%, 30%, 40%, 50%, 60% y 75% con el agregado grueso, para la obtención de una mezcla semi seca con un asentamiento de 0-2”, la caracterización de todos los materiales, y con un diseño de mezclas para cada combinación. En los resultados obtenidos se evidenció, que el RCD en proporciones de 5% y 10%, no influyen significativamente con respecto a la muestra patrón, la cual tenía un diseño de f’c =100kg/cm2 en la resistencia a la compresión, así como también el porcentaje de succión que se obtuvo para estas proporciones fue menor a 20 g/200cm2/min. Haciendo una valoración, estas muestras fluctuaban en el rango de 116 kg/cm2, 127 kg/cm2 y 125 kg/cm2 respectivamente, ubicando a nuestra unidad de ladrillo en un TIPO III de acuerdo con su clasificación según la Norma E.070. Se llegó a constatar que a medida que aumentaba la proporción de RCD la resistencia iba disminuyendo y la dispersión iba aumentando. En el aspecto económico el RCD puede llegar a costar 28.00 soles el m3, más barato que la gravilla de 3/4” con un costo de 35.00 soles el m3, a la fecha se tendría que implementar una planta de chancado de RCD, la cual reduciría el precio de este insumo. En el tema ambiental, el reciclaje de este material ayudará a mitigar en parte la sobre explotación de canteras. Conllevará a favorecer el cuidado del medio ambiente, al generar un uso apropiado de los residuos de construcción y demolición, que vemos a diario formando parte del paisaje de nuestra ciudad
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Influencia de los residuos de construcción y demolición RCD en la resistencia a la compresión y succión del ladrillo de concreto artesanal – Trujillo 2018
Universidad Privada del Norte, 2019Co-Authors: Chávez Ruíz, Ericsson Alen, Nakayo Cabada, Luis AlbertoAbstract:RESUMEN En la presente tesis se investigó la influencia de material reciclado del concreto (RCD) como resultado de la demolición de veredas, que se utilizó en reemplazo de parte del agregado grueso para la elaboración de la mezcla y su aplicación en la fabricación de ladrillos de concreto artesanal. El RCD se obtuvo por proceso de chancado en forma artesanal del desmonte, producto de la demolición de veredas para obtener un TMN de ¾”, el cual se mezcló en proporciones de 0%, 5%, 10%, 20%, 30%, 40%, 50%, 60% y 75% con el agregado grueso, para la obtención de una mezcla semi seca con un asentamiento de 0-2”, la caracterización de todos los materiales, y con un diseño de mezclas para cada combinación. En los resultados obtenidos se evidenció, que el RCD en proporciones de 5% y 10%, no influyen significativamente con respecto a la muestra patrón, la cual tenía un diseño de f’c =100kg/cm2 en la resistencia a la compresión, así como también el porcentaje de succión que se obtuvo para estas proporciones fue menor a 20 g/200cm2/min. Haciendo una valoración, estas muestras fluctuaban en el rango de 116 kg/cm2, 127 kg/cm2 y 125 kg/cm2 respectivamente, ubicando a nuestra unidad de ladrillo en un TIPO III de acuerdo con su clasificación según la Norma E.070. Se llegó a constatar que a medida que aumentaba la proporción de RCD la resistencia iba disminuyendo y la dispersión iba aumentando. En el aspecto económico el RCD puede llegar a costar 28.00 soles el m3, más barato que la gravilla de 3/4” con un costo de 35.00 soles el m3, a la fecha se tendría que implementar una planta de chancado de RCD, la cual reduciría el precio de este insumo. En el tema ambiental, el reciclaje de este material ayudará a mitigar en parte la sobre explotación de canteras. Conllevará a favorecer el cuidado del medio ambiente, al generar un uso apropiado de los residuos de construcción y demolición, que vemos a diario formando parte del paisaje de nuestra ciudad. PALABRAS CLAVES: RCD, proporción de RCD, resistencia a la compresión, succión, agregados, ladrillos de concreto artesanal.ABSTRACT In this thesis, the influence of recycled concrete material (RCD) was investigated as result of the demolition of sidewalks, which was reused to replace part of the coarse aggregate for the preparation of the mixture and its application in the manufacture of concrete bricks. Artisanal. The RCD was obtained by the traditional process of crushing, the resulting from the demolition of paths to obtain a Maximum size nominal TMN of ¾ ", which was mixed in proportions of 5%, 10%, 20%, 30%, 40%, 50%, 60% and 75%, with the coarse aggregate, to obtain a semi-dry mixture with a settlement of 0-2 ", characterization of all the materials, and with a mix design for each combination. The results showed that RCD in proportions of 5% and 10% did not significantly influence the Sample Pattern, which had a design of F'c = 100kg/cm2 in the resistance to compression, as well as the percentage of suction Obtained for these proportions was less than 20 g/200cm2/min. By making an assessment, these Samples fluctuated in the range of 116 kg/cm2, 127 kg/cm2 and 125 kg/cm2 respectively, locating our brick unit in a type III according to its classification according to standard E. 070. It was noted that as the proportion of RCD increased, the resistance was declining, and the dispersion was increasing. In the economic aspect, the RCD can cost 28.00 soles per cubic meter, cheaper than the 3/4 "gravel with a cost of 35.00 soles per m3, to date a RCD crushing plant would have to be implemented, which reduce the price of this input. In the environmental issue, the recycling of this material will help mitigate in part the over-exploitation of quarries. It will lead to fostering the care of the environment by generating an appropriate use of construction and demolition waste, which we see every day as part of the landscape of our city. KEYWORDS: RCD, RCD proportion, compression resistance, suction, aggregates, craft concrete bricks
Stefanie Wienkoop - One of the best experts on this subject based on the ideXlab platform.
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Integration of metabolomic and proteomic phenotypes: analysis of data covariance dissects starch and RFO metabolism from low and high temperature compensation response in Arabidopsis thaliana.
Molecular & cellular proteomics : MCP, 2008Co-Authors: Stefanie Wienkoop, Katja Morgenthal, Florian Wolschin, Matthias Scholz, Joachim Selbig, Wolfram WeckwerthAbstract:Statistical mining and integration of complex molecular data including metabolites, proteins, and transcripts is one of the critical goals of systems biology (Ideker, T., Galitski, T., and Hood, L. (2001) A new approach to decoding life: systems biology. Annu. Rev. Genomics Hum. Genet. 2, 343-372). A number of studies have demonstrated the parallel analysis of metabolites and large scale transcript expression. Protein analysis has been ignored in these studies, although a clear correlation between transcript and protein levels is shown only in rare cases, necessitating that actual protein levels have to be determined for protein function analysis. Here, we present an approach to investigate the combined covariance structure of metabolite and protein dynamics in a systemic response to abiotic temperature stress in Arabidopsis thaliana wild-type and a corresponding starch-deficient mutant (phosphoglucomutase-deficient). Independent component analysis revealed phenotype classification resolving genotype-dependent response effects to temperature treatment and genotype-independent general temperature compensation mechanisms. An observation is the stress-induced increase of raffinose-family-oligosaccharide levels in the absence of transitory starch storage/mobilization in temperature-treated phosphoglucomutase plants indicating that sucrose synthesis and storage in these mutant plants is sufficient to bypass the typical starch storage/mobilization pathways under abiotic stress. Eventually, Sample Pattern recognition and correlation network topology analysis allowed for the detection of specific metabolite-protein co-regulation and assignment of a circadian output regulated RNA-binding protein to these processes. The whole concept of high-dimensional profiling data integration from many replicates, subsequent multivariate statistics for dimensionality reduction, and covariance structure analysis is proposed to be a major strategy for revealing central responses of the biological system under study.
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stable isotope free quantitative shotgun proteomics combined with Sample Pattern recognition for rapid diagnostics
Journal of Separation Science, 2006Co-Authors: Stefanie Wienkoop, Estibaliz Larrainzar, Michaela Niemann, Esther M Gonzalez, Ute Lehmann, Wolfram WeckwerthAbstract:Mass spectrometry (MS) has become a powerful tool for the quantitative analysis of complex protein Samples. A high-throughput strategy for the comparative analysis of multiple protein Samples with high complexity becomes more and more important. Two strategies, spectral count and peak intensity, for label-free MS analysis of prefractionated complex mixtures have been described recently to be useful for quantitation. Here we compare both strategies for rapid and quantitative 1-D shotgun LC/MS/MS analyses of highly complex protein mixtures using silica-based monolithic columns. First, we validated linearity and sensitivity of these methods by spiking varying amounts of an internal standard protein in a complex plant protein extract. Secondly, quantitative data of proteins of Medicago truncatula nodules were visualized with independent components analysis using data either obtained from spectral count or peak integration performed with commercial software. Spectral count showed apparent advantages over peak integration because several peptides per protein are automatically averaged, the linear dynamic range of quantitation increases in complex matrices and the number of quantified proteins surpasses the number of proteins using peak integration. Thus, for the need of rapid comparative analysis of highly complex protein Samples, spectral count enables Sample Pattern recognition and identification of biomarkers in nongel based proteomic studies.
Escajadillo Bello, Hermes Eberardo - One of the best experts on this subject based on the ideXlab platform.
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Efecto in vitro de extractos de membrana de bartonella bacilliformis sobre células de cordón umbilical
Universidad Nacional de Trujillo, 2017Co-Authors: Escajadillo Bello, Hermes EberardoAbstract:El presente trabajo de tesis se realizó con el objetivo de determinar la influencia de los extractos de membrana de Bartonella bacilliformis in vitro sobre células aisladas de cordón umbilical y que por antecedentes registrados, corroborar la posibilidad que éste tiene de estimular la diferenciación de células madres mesenquimales en Linfocitos. Se tomaron 60 muestras de cordón umbilical, con una longitud promedio de 40 cm en viales estériles conteniendo cada uno 20 mL de medio de cultivo celular Waymouth con antibiótico a 100 UI/ mL de Penicilina y 100 ug/mL de Estreptomicina se codificó y se conservó a - 40°C hasta su procesamiento. Los cultivos de Bartonella bacilliformis fueron obtenidas en el laboratorio de Toxicología de la UNT. Se dividieron en cuatro grupos, cada uno con 15 muestras, A, B, y C los grupos problema y D el grupo testigo o blanco. El procesamiento de las muestras se realizó inicialmente haciendo el homogeinizado de los mismos y luego se le añadió 0.5 mL de colagenasa y 2 mL de Waymouth con gentamicina y V gotas de extracto de membrana de Bartonella bacilliformis , no se añadió extracto de membrana a las muestras testigo y se incubaron a 37°C durante 72 horas .,luego se centrifugó a 3000 rpm por 15 minutos , guardando el sobrenadante a -40°C para identificar la IL-2 y con el precipitado se preparaba un frotis dos por cada muestra. La lectura se hacía a 100 x (inmersión), siguiendo la técnica de Almena, se contaron las células en 100 campos microscópicos. Similar procedimiento se hizo con los preparados de las muestras testigos. La identificación de la IL-2 se realizó en los sobrenadantes de la inducción mediante espectrofotometría UV a 582 nm de longitud de onda. Los resultados fueron la identificación de la IL-2 en las muestras, las mismas que presentaron picos similares a la muestra patrón de IL-2 a 582 nm de longitud de onda., y en los campos microscópicos Linfocitos CD4+ como resultado del proceso de transformación de células madres mesenquimales de cordón umbilical en Linfocitos CD4+ por efecto de los extractos de membrana de Bartonella bacilliformis.TesisThe present thesis work was carried out with the objective of determining the influence of extracts of membrane of Bartonella bacilliformis vitro on cells isolated from umbilical cord and that for registered background, corroborate the possibility it has to stimulate the differentiation of stem cells in mesenchymal cells. 60 Samples of umbilical cord, with an average length of 40 cm in sterile vials containing each 20 mL of culture medium cell Waymouth with antibiotic to 100 IU / mL penicillin and 100 ug/mL streptomycin was coded and kept at - 40° C until processing. Crops of Bartonella bacilliformis were obtained in the laboratory of Toxicology of the UNT. They were divided into four groups, each with 15 Samples, A, B, and C the problem groups and (D) the Group witness or white. Processing of the Samples was carried out initially by the homogeinizado of them and then added 0.5 mL collagenase and 2 mL of Waymouth with gentamicin and V drops of extract of membrane of Bartonella bacilliformis, not membrane extract was added to Samples witness and incubated at 37 ° C for 72 hours., then centrifuged at 3000 rpm for 15 minutes storing the supernatant at. The reading was 100 x (DIP), following the technique of Almena, counted the cells in 100 microscopic fields. Similar procedure was done with the prepared Samples witnesses. The identification of the IL-2 was carried out in the supernatants of induction by UV spectrophotometry at 582 nm wavelength. The results were the identification of IL-2 in the Samples, which are presented peaks similar to the Sample Pattern of IL-2 at 582 nm wave length, and microscopic fields CD4 + lymphocytes as a result of the process of transformation of stem cells mesenchymal of umbilical cord in lymphocytes CD4 + by effect of the extracts of membrane of Bartonella bacilliformis
