The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
Xiuguo Hua - One of the best experts on this subject based on the ideXlab platform.
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proteome analysis reveals syndecan 1 regulates porcine Sapelovirus replication
International Journal of Molecular Sciences, 2020Co-Authors: Tingting Zhao, Qi Chen, Li Cui, Zhonghai Zhang, Xiuguo HuaAbstract:Porcine Sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag proteomics analysis to investigate the differentially expressed proteins (DEPs) in PSV-infected pig kidney (PK)-15 cells and explored the interactions between PSV and host cells. Here we mapped 181 DEPs, including 59 up-regulated and 122 down-regulated DEPs. Among them, osteopontin (SPP1), induced protein with tetratricopeptide repeats 5 (IFIT5), ISG15 ubiquitin-like modifier (ISG15), vinculin (VCL), and syndecan-1 (SDC1) were verified significantly changed using RT-qPCR. Additionally, overexpression of SDC1 promoted PSV viral protein (VP)1 synthesis and virus titer, and silencing of SDC1 revealed the opposite results. Our findings show that SDC1 is a novel host protein and plays crucial roles in regulating PSV replication.
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entry of Sapelovirus into ipec j2 cells is dependent on caveolae mediated endocytosis
Virology Journal, 2019Co-Authors: Tingting Zhao, Li Cui, Zhonghai Zhang, Xiaojuan Shen, Xiuguo HuaAbstract:Porcine Sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, are a significant cause of enteritis, pneumonia, polioencephalomyelitis and reproductive disorders in pigs. However, the life cycle of PSV on the molecular level is largely unknown. Here, we used chemical inhibitors, RNA interference, and overexpression of dominant negative (DN) mutant plasmids to verify the roles of distinct endocytic pathways involved in PSV entry into porcine small intestinal epithelial cell line (IPEC-J2). Our experiments indicated that PSV infection was inhibited when cells were pre-treated with NH4Cl or chloroquine. Inhibitors nystatin, methyl-β-cyclodextrin, dynasore and wortmannin dramatically reduced PSV entry efficiency, whereas the inhibitors chlorpromazine and EIPA had no effect. Furthermore, overexpression caveolin DN mutant and siRNA against caveolin also decreased virus titers and VP1 protein synthesis, whereas overexpression EPS15 DN mutant and siRNA against EPS15 did not reduce virus infection. Our findings suggest that PSV entry into IPEC-J2 cells depends on caveolae/lipid raft mediated-endocytosis, that is pH-dependent and requires dynamin and PI3K but is independent of clathrin and macropinocytosis.
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porcine Sapelovirus enters pk 15 cells via caveolae dependent endocytosis and requires rab7 and rab11
Virology, 2019Co-Authors: Tingting Zhao, Li Cui, Zhonghai Zhang, Xiaojuan Shen, Xiuguo HuaAbstract:To comprehensively understand the endocytosis of Sapelovirus A (PSV) entry into PK-15 cells, we studied PSV infection in the context of cell perturbations through drug inhibition, siRNA silencing and overexpression of dominant negative (DN) mutants. We showed here that PSV infection of PK-15 cells was unaffected by pretreated with chlorpromazine, EIPA, knockdown of the clathrin heavy chain or overexpression of Eps15 DN mutant. Conversely, PSV infection was sensitive to NH4Cl, chloroquine, dynasore, nystatin, MβCD and wortmannin with reduced PSV VP1 expression levels and virus titer. Additionally, PSV invasion leaded to rapid actin rearrangement and disruption of the cellular actin network enhanced PSV infection. After internalization the virus was transported to late endosomes and/or cycling endosomes that requires the participation of Rab7 and Rab11. Our findings demonstrate that PSV uses caveolae-dependent endocytosis as the predominant entry portal into PK-15 cells which requires low pH, dynamin, Rab7 and Rab11.
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Entry of Sapelovirus into IPEC-J2 cells is dependent on caveolae-mediated endocytosis
BMC, 2019Co-Authors: Tingting Zhao, Li Cui, Zhonghai Zhang, Xiaojuan Shen, Xiuguo HuaAbstract:Abstract Background Porcine Sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, are a significant cause of enteritis, pneumonia, polioencephalomyelitis and reproductive disorders in pigs. However, the life cycle of PSV on the molecular level is largely unknown. Methods Here, we used chemical inhibitors, RNA interference, and overexpression of dominant negative (DN) mutant plasmids to verify the roles of distinct endocytic pathways involved in PSV entry into porcine small intestinal epithelial cell line (IPEC-J2). Results Our experiments indicated that PSV infection was inhibited when cells were pre-treated with NH4Cl or chloroquine. Inhibitors nystatin, methyl-β-cyclodextrin, dynasore and wortmannin dramatically reduced PSV entry efficiency, whereas the inhibitors chlorpromazine and EIPA had no effect. Furthermore, overexpression caveolin DN mutant and siRNA against caveolin also decreased virus titers and VP1 protein synthesis, whereas overexpression EPS15 DN mutant and siRNA against EPS15 did not reduce virus infection. Conclusions Our findings suggest that PSV entry into IPEC-J2 cells depends on caveolae/lipid raft mediated-endocytosis, that is pH-dependent and requires dynamin and PI3K but is independent of clathrin and macropinocytosis
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optimal expression and purification of Sapelovirus a structural protein vp1 and its immunogenicity in mice
Polish Journal of Veterinary Sciences, 2018Co-Authors: Tingting Zhao, Li Cui, Q L Liang, Z H Zhang, Xiuguo HuaAbstract:Sapelovirus A (SV-A) is a positive-sense single-stranded RNA virus which is associated with acute diarrhea, pneumonia and reproductive disorders. The virus capsid is composed of four proteins, and the functions of the structural proteins are unclear. In this study, we expressed SV-A structural protein VP1 and studied its antigenicity and immunogenicity. SDS-PAGE analysis revealed that the target gene was expressed at high levels at 0.6 mM concentration of IPTG for 24 h. The mouse polyclonal antibody against SV-A VP1 protein was produced and reached a high antiserum titer (1: 2,048,000). Immunized mice sera with the recombinant SV-A VP1 protein showed specific recognition of purified VP1 protein by western blot assay and could recognize native SV-A VP1 protein in PK-15 cells infected with SV-A by indirect immunofluorescence assay. The successfully purified recombinant protein was able to preserve its antigenic determinants and the generated mouse anti-SV-A VP1 antibodies could recognize native SV-A, which may have the potential to be used to detect SV-A infection in pigs.
Deok-song Kim - One of the best experts on this subject based on the ideXlab platform.
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occurrence and molecular characterization of Sapelovirus a in diarrhea and non diarrhea feces of different age group pigs in one korean pig farm
Journal of Veterinary Medical Science, 2016Co-Authors: Geonyong Bak, Kyu-yeol Son, Deok-song Kim, Mun-il Kang, Jiyun Kim, Mia Madel Alfajaro, Jungyu Park, Mahmoud Soliman, Jayoung Seo, Yeongbin BaekAbstract:To determine the occurrence and genetic diversity of Sapelovirus A (SV-A) in diarrhea and non-diarrhea feces of Korean pigs, 110 specimens from different age groups of pigs in the same farm were analyzed by RT-nested PCR. SV-As were detected in 60% of both diarrhea and non-diarrhea specimens regardless of age groups with primer pairs for 2C region, in which all diarrhea samples were co-infected by other enteric pathogens. Phylogenetical analysis of partial VP1 region showed that our strains and several other Korean strains belonged to cluster I, distinct from some strains reported in Korea and other countries. These data indicate that genetically distinct SV-As are frequently detected in Korean pigs irrespective of diarrhea and age.
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porcine Sapelovirus uses α2 3 linked sialic acid on gd1a ganglioside as a receptor
Journal of Virology, 2016Co-Authors: Deok-song Kim, Kyu-yeol Son, Kyungmin Koo, Jiyun Kim, Mia Madel Alfajaro, Jungyu Park, Myra Hosmillo, Mahmoud Soliman, Yeongbin Baek, Eunhyo ChoAbstract:ABSTRACT The receptor(s) for porcine Sapelovirus (PSV), which causes diarrhea, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs, remains largely unknown. Given the precedent for other picornaviruses which use terminal sialic acids (SAs) as receptors, we examined the role of SAs in PSV binding and infection. Using a variety of approaches, including treating cells with a carbohydrate-destroying chemical (NaIO 4 ), mono- or oligosaccharides ( N -acetylneuraminic acid, galactose, and 6′-sialyllactose), linkage-specific sialidases (neuraminidase and sialidase S), lectins (Maakia amurensis lectin and Sambucus nigra lectin), proteases (trypsin and chymotrypsin), and glucosylceramide synthase inhibitors (dl- threo -1-phenyl-2-decanoylamino-3-morpholino-1-propanol and phospholipase C), we demonstrated that PSV could recognize α2,3-linked SA on glycolipids as a receptor. On the other hand, PSVs had no binding affinity for synthetic histo-blood group antigens (HBGAs), suggesting that PSVs could not use HBGAs as receptors. Depletion of cell surface glycolipids followed by reconstitution studies indicated that GD1a ganglioside, but not other gangliosides, could restore PSV binding and infection, further confirming α2,3-linked SA on GD1a as a PSV receptor. Our results could provide significant information on the understanding of the life cycle of Sapelovirus and other picornaviruses. For the broader community in the area of pathogens and pathogenesis, these findings and insights could contribute to the development of affordable, useful, and efficient drugs for anti-Sapelovirus therapy. IMPORTANCE The porcine Sapelovirus (PSV) is known to cause enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. However, the receptor(s) that the PSV utilizes to enter host cells remains largely unknown. Using a variety of approaches, we showed that α2,3-linked terminal sialic acid (SA) on the cell surface GD1a ganglioside could be used for PSV binding and infection as a receptor. On the other hand, histo-blood group antigens also present in the cell surface carbohydrates could not be utilized as PSV receptors for binding and infection. These findings should contribute to the understanding of the Sapelovirus life cycle and to the development of affordable, useful and efficient drugs for anti-Sapelovirus therapy.
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porcine Sapelovirus uses α2 3 linked sialic acid on gd1a ganglioside as a functional receptor
2016Co-Authors: Deok-song Kim, Kyu-yeol Son, Kyungmin Koo, Jiyun Kim, Mia Madel Alfajaro, Jungyu Park, Myra Hosmillo, Mahmoud Soliman, Yeongbin Baek, Eunhyo ChoAbstract:This study was supported by Wellcome Trust (097997/Z/11/Z) and a grant from Basic Science Research Program through the National Research Foundation of Korea (NRF). This study was also supported by Bio-industry Technology Development Program through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (iPET) funded by the Ministry of Agriculture, Food and Rural Affairs, and Chonnam National University (2013). IG is a Wellcome Senior Fellow supported by the Wellcome Trust (097997/Z/11/Z).
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full length genomic analysis of korean porcine Sapelovirus strains
PLOS ONE, 2014Co-Authors: Kyu-yeol Son, Graham J. Belsham, Deok-song Kim, Joseph Kwon, Jong-soon Choi, Mun-il Kang, Kyoung-oh ChoAbstract:Porcine Sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, is associated with diarrhea, pneumonia, severe neurological disorders, and reproductive failure in pigs. However, the structural features of the complete PSV genome remain largely unknown. To analyze the structural features of PSV genomes, the full-length nucleotide sequences of three Korean PSV strains were determined and analyzed using bioinformatic techniques in comparison with other known PSV strains. The Korean PSV genomes ranged from 7,542 to 7,566 nucleotides excluding the 3′ poly(A) tail, and showed the typical picornavirus genome organization; 5′untranslated region (UTR)-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3′UTR. Three distinct cis-active RNA elements, the internal ribosome entry site (IRES) in the 5′UTR, a cis-replication element (CRE) in the 2C coding region and 3′UTR were identified and their structures were predicted. Interestingly, the structural features of the CRE and 3′UTR were different between PSV strains. The availability of these first complete genome sequences for PSV strains will facilitate future investigations of the molecular pathogenesis and evolutionary characteristics of PSV.
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Comparison of complete nucleotide and deduced amino acid sequences between the Korean porcine Sapelovirus (PSV) strains and other known picornavirus strains.
2014Co-Authors: Kyu-yeol Son, Graham J. Belsham, Deok-song Kim, Joseph Kwon, Jong-soon Choi, Mun-il Kang, Kyoung-oh ChoAbstract:1GenBank accession numbers of strains used are in Table S2.2The full-length nucleotide sequence identities among PSVs and other picornaviruses.3The full-length deduced amino acid sequence identities among PSVs and other picornaviruses.4ASV: avian Sapelovirus.5SSV: simian Sapelovirus.6PTV-1: porcine teschovirus serotype 1.7PEV-9: porcine enterovirus serotype 9.8EMCV: encephalomyocarditis virus.9FMDV: foot-and-mouth disease virus type O.10ERBV-1: Equine rhinitis B virus serotype 1.11PV-1: Poliovirus serotype 1(Human enterovirus C serotype).12HAV: Hepatitis A virus.13AiV: Aichi virus.14HPeV-1: Human parechovirus serotype 1.15DHVA: Duck hepatitis A virus.16SVV: Seneca Valley virus.17AEV: Avian encephalomyelitis virus.Comparison of complete nucleotide and deduced amino acid sequences between the Korean porcine Sapelovirus (PSV) strains and other known picornavirus strains.
Li Cui - One of the best experts on this subject based on the ideXlab platform.
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proteome analysis reveals syndecan 1 regulates porcine Sapelovirus replication
International Journal of Molecular Sciences, 2020Co-Authors: Tingting Zhao, Qi Chen, Li Cui, Zhonghai Zhang, Xiuguo HuaAbstract:Porcine Sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag proteomics analysis to investigate the differentially expressed proteins (DEPs) in PSV-infected pig kidney (PK)-15 cells and explored the interactions between PSV and host cells. Here we mapped 181 DEPs, including 59 up-regulated and 122 down-regulated DEPs. Among them, osteopontin (SPP1), induced protein with tetratricopeptide repeats 5 (IFIT5), ISG15 ubiquitin-like modifier (ISG15), vinculin (VCL), and syndecan-1 (SDC1) were verified significantly changed using RT-qPCR. Additionally, overexpression of SDC1 promoted PSV viral protein (VP)1 synthesis and virus titer, and silencing of SDC1 revealed the opposite results. Our findings show that SDC1 is a novel host protein and plays crucial roles in regulating PSV replication.
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entry of Sapelovirus into ipec j2 cells is dependent on caveolae mediated endocytosis
Virology Journal, 2019Co-Authors: Tingting Zhao, Li Cui, Zhonghai Zhang, Xiaojuan Shen, Xiuguo HuaAbstract:Porcine Sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, are a significant cause of enteritis, pneumonia, polioencephalomyelitis and reproductive disorders in pigs. However, the life cycle of PSV on the molecular level is largely unknown. Here, we used chemical inhibitors, RNA interference, and overexpression of dominant negative (DN) mutant plasmids to verify the roles of distinct endocytic pathways involved in PSV entry into porcine small intestinal epithelial cell line (IPEC-J2). Our experiments indicated that PSV infection was inhibited when cells were pre-treated with NH4Cl or chloroquine. Inhibitors nystatin, methyl-β-cyclodextrin, dynasore and wortmannin dramatically reduced PSV entry efficiency, whereas the inhibitors chlorpromazine and EIPA had no effect. Furthermore, overexpression caveolin DN mutant and siRNA against caveolin also decreased virus titers and VP1 protein synthesis, whereas overexpression EPS15 DN mutant and siRNA against EPS15 did not reduce virus infection. Our findings suggest that PSV entry into IPEC-J2 cells depends on caveolae/lipid raft mediated-endocytosis, that is pH-dependent and requires dynamin and PI3K but is independent of clathrin and macropinocytosis.
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porcine Sapelovirus enters pk 15 cells via caveolae dependent endocytosis and requires rab7 and rab11
Virology, 2019Co-Authors: Tingting Zhao, Li Cui, Zhonghai Zhang, Xiaojuan Shen, Xiuguo HuaAbstract:To comprehensively understand the endocytosis of Sapelovirus A (PSV) entry into PK-15 cells, we studied PSV infection in the context of cell perturbations through drug inhibition, siRNA silencing and overexpression of dominant negative (DN) mutants. We showed here that PSV infection of PK-15 cells was unaffected by pretreated with chlorpromazine, EIPA, knockdown of the clathrin heavy chain or overexpression of Eps15 DN mutant. Conversely, PSV infection was sensitive to NH4Cl, chloroquine, dynasore, nystatin, MβCD and wortmannin with reduced PSV VP1 expression levels and virus titer. Additionally, PSV invasion leaded to rapid actin rearrangement and disruption of the cellular actin network enhanced PSV infection. After internalization the virus was transported to late endosomes and/or cycling endosomes that requires the participation of Rab7 and Rab11. Our findings demonstrate that PSV uses caveolae-dependent endocytosis as the predominant entry portal into PK-15 cells which requires low pH, dynamin, Rab7 and Rab11.
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Entry of Sapelovirus into IPEC-J2 cells is dependent on caveolae-mediated endocytosis
BMC, 2019Co-Authors: Tingting Zhao, Li Cui, Zhonghai Zhang, Xiaojuan Shen, Xiuguo HuaAbstract:Abstract Background Porcine Sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, are a significant cause of enteritis, pneumonia, polioencephalomyelitis and reproductive disorders in pigs. However, the life cycle of PSV on the molecular level is largely unknown. Methods Here, we used chemical inhibitors, RNA interference, and overexpression of dominant negative (DN) mutant plasmids to verify the roles of distinct endocytic pathways involved in PSV entry into porcine small intestinal epithelial cell line (IPEC-J2). Results Our experiments indicated that PSV infection was inhibited when cells were pre-treated with NH4Cl or chloroquine. Inhibitors nystatin, methyl-β-cyclodextrin, dynasore and wortmannin dramatically reduced PSV entry efficiency, whereas the inhibitors chlorpromazine and EIPA had no effect. Furthermore, overexpression caveolin DN mutant and siRNA against caveolin also decreased virus titers and VP1 protein synthesis, whereas overexpression EPS15 DN mutant and siRNA against EPS15 did not reduce virus infection. Conclusions Our findings suggest that PSV entry into IPEC-J2 cells depends on caveolae/lipid raft mediated-endocytosis, that is pH-dependent and requires dynamin and PI3K but is independent of clathrin and macropinocytosis
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optimal expression and purification of Sapelovirus a structural protein vp1 and its immunogenicity in mice
Polish Journal of Veterinary Sciences, 2018Co-Authors: Tingting Zhao, Li Cui, Q L Liang, Z H Zhang, Xiuguo HuaAbstract:Sapelovirus A (SV-A) is a positive-sense single-stranded RNA virus which is associated with acute diarrhea, pneumonia and reproductive disorders. The virus capsid is composed of four proteins, and the functions of the structural proteins are unclear. In this study, we expressed SV-A structural protein VP1 and studied its antigenicity and immunogenicity. SDS-PAGE analysis revealed that the target gene was expressed at high levels at 0.6 mM concentration of IPTG for 24 h. The mouse polyclonal antibody against SV-A VP1 protein was produced and reached a high antiserum titer (1: 2,048,000). Immunized mice sera with the recombinant SV-A VP1 protein showed specific recognition of purified VP1 protein by western blot assay and could recognize native SV-A VP1 protein in PK-15 cells infected with SV-A by indirect immunofluorescence assay. The successfully purified recombinant protein was able to preserve its antigenic determinants and the generated mouse anti-SV-A VP1 antibodies could recognize native SV-A, which may have the potential to be used to detect SV-A infection in pigs.
Kyu-yeol Son - One of the best experts on this subject based on the ideXlab platform.
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occurrence and molecular characterization of Sapelovirus a in diarrhea and non diarrhea feces of different age group pigs in one korean pig farm
Journal of Veterinary Medical Science, 2016Co-Authors: Geonyong Bak, Kyu-yeol Son, Deok-song Kim, Mun-il Kang, Jiyun Kim, Mia Madel Alfajaro, Jungyu Park, Mahmoud Soliman, Jayoung Seo, Yeongbin BaekAbstract:To determine the occurrence and genetic diversity of Sapelovirus A (SV-A) in diarrhea and non-diarrhea feces of Korean pigs, 110 specimens from different age groups of pigs in the same farm were analyzed by RT-nested PCR. SV-As were detected in 60% of both diarrhea and non-diarrhea specimens regardless of age groups with primer pairs for 2C region, in which all diarrhea samples were co-infected by other enteric pathogens. Phylogenetical analysis of partial VP1 region showed that our strains and several other Korean strains belonged to cluster I, distinct from some strains reported in Korea and other countries. These data indicate that genetically distinct SV-As are frequently detected in Korean pigs irrespective of diarrhea and age.
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porcine Sapelovirus uses α2 3 linked sialic acid on gd1a ganglioside as a receptor
Journal of Virology, 2016Co-Authors: Deok-song Kim, Kyu-yeol Son, Kyungmin Koo, Jiyun Kim, Mia Madel Alfajaro, Jungyu Park, Myra Hosmillo, Mahmoud Soliman, Yeongbin Baek, Eunhyo ChoAbstract:ABSTRACT The receptor(s) for porcine Sapelovirus (PSV), which causes diarrhea, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs, remains largely unknown. Given the precedent for other picornaviruses which use terminal sialic acids (SAs) as receptors, we examined the role of SAs in PSV binding and infection. Using a variety of approaches, including treating cells with a carbohydrate-destroying chemical (NaIO 4 ), mono- or oligosaccharides ( N -acetylneuraminic acid, galactose, and 6′-sialyllactose), linkage-specific sialidases (neuraminidase and sialidase S), lectins (Maakia amurensis lectin and Sambucus nigra lectin), proteases (trypsin and chymotrypsin), and glucosylceramide synthase inhibitors (dl- threo -1-phenyl-2-decanoylamino-3-morpholino-1-propanol and phospholipase C), we demonstrated that PSV could recognize α2,3-linked SA on glycolipids as a receptor. On the other hand, PSVs had no binding affinity for synthetic histo-blood group antigens (HBGAs), suggesting that PSVs could not use HBGAs as receptors. Depletion of cell surface glycolipids followed by reconstitution studies indicated that GD1a ganglioside, but not other gangliosides, could restore PSV binding and infection, further confirming α2,3-linked SA on GD1a as a PSV receptor. Our results could provide significant information on the understanding of the life cycle of Sapelovirus and other picornaviruses. For the broader community in the area of pathogens and pathogenesis, these findings and insights could contribute to the development of affordable, useful, and efficient drugs for anti-Sapelovirus therapy. IMPORTANCE The porcine Sapelovirus (PSV) is known to cause enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. However, the receptor(s) that the PSV utilizes to enter host cells remains largely unknown. Using a variety of approaches, we showed that α2,3-linked terminal sialic acid (SA) on the cell surface GD1a ganglioside could be used for PSV binding and infection as a receptor. On the other hand, histo-blood group antigens also present in the cell surface carbohydrates could not be utilized as PSV receptors for binding and infection. These findings should contribute to the understanding of the Sapelovirus life cycle and to the development of affordable, useful and efficient drugs for anti-Sapelovirus therapy.
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porcine Sapelovirus uses α2 3 linked sialic acid on gd1a ganglioside as a functional receptor
2016Co-Authors: Deok-song Kim, Kyu-yeol Son, Kyungmin Koo, Jiyun Kim, Mia Madel Alfajaro, Jungyu Park, Myra Hosmillo, Mahmoud Soliman, Yeongbin Baek, Eunhyo ChoAbstract:This study was supported by Wellcome Trust (097997/Z/11/Z) and a grant from Basic Science Research Program through the National Research Foundation of Korea (NRF). This study was also supported by Bio-industry Technology Development Program through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (iPET) funded by the Ministry of Agriculture, Food and Rural Affairs, and Chonnam National University (2013). IG is a Wellcome Senior Fellow supported by the Wellcome Trust (097997/Z/11/Z).
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full length genomic analysis of korean porcine Sapelovirus strains
PLOS ONE, 2014Co-Authors: Kyu-yeol Son, Graham J. Belsham, Deok-song Kim, Joseph Kwon, Jong-soon Choi, Mun-il Kang, Kyoung-oh ChoAbstract:Porcine Sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, is associated with diarrhea, pneumonia, severe neurological disorders, and reproductive failure in pigs. However, the structural features of the complete PSV genome remain largely unknown. To analyze the structural features of PSV genomes, the full-length nucleotide sequences of three Korean PSV strains were determined and analyzed using bioinformatic techniques in comparison with other known PSV strains. The Korean PSV genomes ranged from 7,542 to 7,566 nucleotides excluding the 3′ poly(A) tail, and showed the typical picornavirus genome organization; 5′untranslated region (UTR)-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3′UTR. Three distinct cis-active RNA elements, the internal ribosome entry site (IRES) in the 5′UTR, a cis-replication element (CRE) in the 2C coding region and 3′UTR were identified and their structures were predicted. Interestingly, the structural features of the CRE and 3′UTR were different between PSV strains. The availability of these first complete genome sequences for PSV strains will facilitate future investigations of the molecular pathogenesis and evolutionary characteristics of PSV.
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Comparison of complete nucleotide and deduced amino acid sequences between the Korean porcine Sapelovirus (PSV) strains and other known picornavirus strains.
2014Co-Authors: Kyu-yeol Son, Graham J. Belsham, Deok-song Kim, Joseph Kwon, Jong-soon Choi, Mun-il Kang, Kyoung-oh ChoAbstract:1GenBank accession numbers of strains used are in Table S2.2The full-length nucleotide sequence identities among PSVs and other picornaviruses.3The full-length deduced amino acid sequence identities among PSVs and other picornaviruses.4ASV: avian Sapelovirus.5SSV: simian Sapelovirus.6PTV-1: porcine teschovirus serotype 1.7PEV-9: porcine enterovirus serotype 9.8EMCV: encephalomyocarditis virus.9FMDV: foot-and-mouth disease virus type O.10ERBV-1: Equine rhinitis B virus serotype 1.11PV-1: Poliovirus serotype 1(Human enterovirus C serotype).12HAV: Hepatitis A virus.13AiV: Aichi virus.14HPeV-1: Human parechovirus serotype 1.15DHVA: Duck hepatitis A virus.16SVV: Seneca Valley virus.17AEV: Avian encephalomyelitis virus.Comparison of complete nucleotide and deduced amino acid sequences between the Korean porcine Sapelovirus (PSV) strains and other known picornavirus strains.
Eunhyo Cho - One of the best experts on this subject based on the ideXlab platform.
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porcine Sapelovirus uses α2 3 linked sialic acid on gd1a ganglioside as a receptor
Journal of Virology, 2016Co-Authors: Deok-song Kim, Kyu-yeol Son, Kyungmin Koo, Jiyun Kim, Mia Madel Alfajaro, Jungyu Park, Myra Hosmillo, Mahmoud Soliman, Yeongbin Baek, Eunhyo ChoAbstract:ABSTRACT The receptor(s) for porcine Sapelovirus (PSV), which causes diarrhea, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs, remains largely unknown. Given the precedent for other picornaviruses which use terminal sialic acids (SAs) as receptors, we examined the role of SAs in PSV binding and infection. Using a variety of approaches, including treating cells with a carbohydrate-destroying chemical (NaIO 4 ), mono- or oligosaccharides ( N -acetylneuraminic acid, galactose, and 6′-sialyllactose), linkage-specific sialidases (neuraminidase and sialidase S), lectins (Maakia amurensis lectin and Sambucus nigra lectin), proteases (trypsin and chymotrypsin), and glucosylceramide synthase inhibitors (dl- threo -1-phenyl-2-decanoylamino-3-morpholino-1-propanol and phospholipase C), we demonstrated that PSV could recognize α2,3-linked SA on glycolipids as a receptor. On the other hand, PSVs had no binding affinity for synthetic histo-blood group antigens (HBGAs), suggesting that PSVs could not use HBGAs as receptors. Depletion of cell surface glycolipids followed by reconstitution studies indicated that GD1a ganglioside, but not other gangliosides, could restore PSV binding and infection, further confirming α2,3-linked SA on GD1a as a PSV receptor. Our results could provide significant information on the understanding of the life cycle of Sapelovirus and other picornaviruses. For the broader community in the area of pathogens and pathogenesis, these findings and insights could contribute to the development of affordable, useful, and efficient drugs for anti-Sapelovirus therapy. IMPORTANCE The porcine Sapelovirus (PSV) is known to cause enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. However, the receptor(s) that the PSV utilizes to enter host cells remains largely unknown. Using a variety of approaches, we showed that α2,3-linked terminal sialic acid (SA) on the cell surface GD1a ganglioside could be used for PSV binding and infection as a receptor. On the other hand, histo-blood group antigens also present in the cell surface carbohydrates could not be utilized as PSV receptors for binding and infection. These findings should contribute to the understanding of the Sapelovirus life cycle and to the development of affordable, useful and efficient drugs for anti-Sapelovirus therapy.
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porcine Sapelovirus uses α2 3 linked sialic acid on gd1a ganglioside as a functional receptor
2016Co-Authors: Deok-song Kim, Kyu-yeol Son, Kyungmin Koo, Jiyun Kim, Mia Madel Alfajaro, Jungyu Park, Myra Hosmillo, Mahmoud Soliman, Yeongbin Baek, Eunhyo ChoAbstract:This study was supported by Wellcome Trust (097997/Z/11/Z) and a grant from Basic Science Research Program through the National Research Foundation of Korea (NRF). This study was also supported by Bio-industry Technology Development Program through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (iPET) funded by the Ministry of Agriculture, Food and Rural Affairs, and Chonnam National University (2013). IG is a Wellcome Senior Fellow supported by the Wellcome Trust (097997/Z/11/Z).
