The Experts below are selected from a list of 69 Experts worldwide ranked by ideXlab platform
P. Sivasubramanian - One of the best experts on this subject based on the ideXlab platform.
-
localization of leucokinin like immunoreactive neurons and their metamorphic changes in the ventral ganglion of the fly Sarcophaga bullata
Comparative Biochemistry and Physiology Part A: Physiology, 1994Co-Authors: P. SivasubramanianAbstract:Abstract The distribution of leucokinin I-like immunoreactive neurons in the ventral ganglion of the fly Sarcophaga bullata was examined by indirect immunofluorescence. In the larval ventral ganglion there are seven pairs of large, highly immunoreactive neurons distributed ventrolaterally as bilateral pairs in abdominal neuromeres 1–7. During metamorphosis, the seven pairs of larval immunoreactive neurons survive and three additional pairs of immunoreactive neurons appear within the condensed abdominal ganglion, bringing the total number of immunoreactive neurons to 10 pairs. It is suggested that the neuropeptide from the newly formed three pairs of leucokinin-like immunoreactive neurons may have some unique function in the life of the adult insect.
-
FMRFamide-like immunoreactivity in the stomatogastric nervous system innervating the gut of the fly, Sarcophaga bullata
Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1992Co-Authors: P. SivasubramanianAbstract:Abstract 1. Distribution of FMRFamide-like peptide activity was examined in the stomatogastric nervous system of the adult fly, Sarcophaga bullata by the indirect immunofluorescent method. 2. The neurons of the hypocerebral ganglion exhibit intense immunoreactivity and extend a thick axon bundle ventrally towards the proventriculus and crop. 3. Near the mouth of the stomodeal valve a dense network of radial and circular immunoreactive processes branch off and innervate the proventriculus. 4. Beyond the proventriculus, the crop duct and anterior midgut wall are also innervated by the FMRFamide-like immunoreactive processes of the nerve from the hypocerebral ganglion. 5. From the pattern of innervation of the gut by FMRFamide-like immunoreactive processes it is suggested that this neuropeptide may regulate feeding activities in the adult fly.
-
Localization of FMRFamide-like immunoreactivity in the larval midgut of the fly, Sarcophaga bullata
Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1992Co-Authors: P. SivasubramanianAbstract:Abstract 1. Distribution of FMRFamide-like immunoreactivity was examined in the larval gut of the fly Sarcophaga bullata . 2. Peroxidase-antiperoxidase and indirect immunofluorescent methods revealed immunoreactivity in the proventriculus and parts of the anterior midgut. 3. The outer wall of the proventriculus was innervated by 12–16 FLI neurons of hypocerebral ganglion. 4. In the anterior midgut, FLI cells were randomly distributed between the muscle fibers and among the columnar epithelial cells of the midgut. 5. The organization, distribution pattern and the association of FLI cells with midgut epithelium suggest that FMRFamide-like peptides may regulate feeding and peristalsis in this insect.
-
fmrfamide like immunoreactivity in the ventral ganglion of the fly Sarcophaga bullata metamorphic changes
Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1991Co-Authors: P. SivasubramanianAbstract:Abstract 1.Localization of FMRFamide-like immunoreactivity was examined in the ventral ganglion of the fly Sarcophaga bullata using the indirect immunofluorescent method. 2. There are six large cells in the thoracic ganglion which are highly immunoreactive at all stages of development. 3. During metamorphosis the thoracic FLI neurons shift their position from ventrolateral to mid-ventral position and their axons terminate and elaborate a highly immunoreactvie dorsal neutral sheath. 4. It is suggested that the dorsal neural sheath may function as a neurohaemal organ from which FMRFamide-like substances may be released into the haemolymph to act as neurohormones.
-
Substance P-like immunoreactive neurons in the adult nervous system of the fly, Sarcophaga bullata.
Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1990Co-Authors: P. SivasubramanianAbstract:Abstract 1. The distribution of substance P -like immunoreactivity was investigated in adult CNS of the fieshfly Sarcophaga bullata using peroxidase-antiperoxidase and indirect immunofluorescent methods. 2. Ten cells in the brain and six in the thoracic ganglion exhibited positive immunoreactivity. 3. The dorsal neural sheath of the thoracic ganglion was highly immunoreactive with a large number of varicosities. 4. It is suggested that substance P may function as a neurohormone/neuromodulator in the fleshfly.
David L Denlinger - One of the best experts on this subject based on the ideXlab platform.
-
genome and ontogenetic based transcriptomic analyses of the flesh fly Sarcophaga bullata
G3: Genes Genomes Genetics, 2019Co-Authors: Ellen O Martinson, John H Werren, Justin T Peyton, Yogeshwar D Kelkar, Emily C Jennings, Joshua B Benoit, David L DenlingerAbstract:The flesh fly, Sarcophaga bullata, is a widely-used model for examining the physiology of insect diapause, development, stress tolerance, neurobiology, and host-parasitoid interactions. Flies in this taxon are implicated in myiasis (larval infection of vertebrates) and feed on carrion, aspects that are important in forensic studies. Here we present the genome of S. bullata, along with developmental- and reproduction-based RNA-Seq analyses. We predict 15,768 protein coding genes, identify orthology in relation to closely related flies, and establish sex and developmental-specific gene sets based on our RNA-Seq analyses. Genomic sequences, predicted genes, and sequencing data sets have been deposited at the National Center for Biotechnology Information. Our results provide groundwork for genomic studies that will expand the flesh fly’s utility as a model system.
-
mendelian inheritance of pupal diapause in the flesh fly Sarcophaga bullata
Journal of Heredity, 2009Co-Authors: David L DenlingerAbstract:Pupal diapause (dormancy) in the flesh fly, Sarcophaga bullata, is induced by short-day photoperiods and low temperature. In this study, the inheritance mode of diapause was investigated by crossing a nondiapausing (nd) strain of S. bullata with 2 diapausing strains having different diapause capacities. The results consistently indicated that diapause incidence is inherited in a simple Mendelian pattern, thus a single gene or a small gene cluster linked to the photoperiodic clock controls the seasonal response of diapause. The fact that the nd strain lacked daily rhythmicity in adult eclosion and showed altered expression of 2 circadian clock genes suggests that the photoperiodic and circadian clocks are related through a shared molecular component in S. bullata.
K. Biemann - One of the best experts on this subject based on the ideXlab platform.
-
amino acid sequence of a protease inhibitor isolated from Sarcophaga bullata determined by mass spectrometry
Protein Science, 2008Co-Authors: Ioannis A Papayannopoulos, K. BiemannAbstract:The amino acid sequence of a protease inhibitor isolated from the hemolymph of Sarcophaga bullata larvae was determined by tandem mass spectrometry. Homology considerations with respect to other protease inhibitors with known primary structures assisted in the choice of the procedure followed in the sequence determination and in the alignment of the various peptides obtained from specific chemical cleavage at cysteines and enzyme digests of the S. bullata protease inhibitor. The resulting sequence of 57 residues is as follows: Val Asp Lys Ser Ala Cys Leu Gln Pro Lys Glu Val Gly Pro Cys Arg Lys Ser Asp Phe Val Phe Phe Tyr Asn Ala Asp Thr Lys Ala Cys Glu Glu Phe Leu Tyr Gly Gly Cys Arg Gly Asn Asp Asn Arg Phe Asn Thr Lys Glu Glu Cys Glu Lys Leu Cys Leu.
-
Amino acid sequence of a protease inhibitor isolated from Sarcophaga bullata determined w
1992Co-Authors: K. BiemannAbstract:The amino acid sequence of a protease inhibitor isolated from the hemolymph of Sarcophaga bullata larvae was determined by tandem mass spectrometry. Homology considerations with respect to other protease inhibitors with known primary structures assisted in the choice of the procedure followed in the sequence determination and in the alignment of the various peptides obtained from specific chemical cleavage at cysteines and enzyme digests of the S. bullata protease inhibitor. The resulting sequence of 57 residues is as follows: Val Asp Lys Ser Ala Cys Leu Gln Pro Lys Glu Val Gly Pro Cys Arg Lys Ser Asp Phe Val Phe Phe Tyr Asn Ala Asp Thr Lys Ala Cys Glu Glu Phe Leu Tyr Gly Gly Cys Arg Gly Asn Asp Asn Arg Phe Asn Thr Lys Glu Glu Cys Glu Lys Leu Cys Leu.
P J Albert - One of the best experts on this subject based on the ideXlab platform.
-
behavioural and gustatory responses of female neobellieria Sarcophaga bullata to sucrose and amino acids
Physiological Entomology, 1997Co-Authors: P J Albert, N Banga, E DolzanAbstract:. Unmated female Neobellieria (=Sarcophaga) bullata (Parker) (Diptera: Sarcophagidae) of different ages ranging from 1 to 24 days consistently preferred a solution of 100 mM/1 sucrose to one containing a mixture of six amino acids at a total concentration of 10 mM/1. When these solutions were used to stimulate the gustatory sensilla on the proboscis of 2- and 5-day-old flies, sucrose elicited a greater response from cells 1 and 2 in large and cell 1 in medium-length hairs, which could account for the feeding preference of the flies for this chemical.
-
differences in response patterns of labellar chemosensilla of the fleshfly Sarcophaga bullata
Comparative Biochemistry and Physiology Part A: Physiology, 1991Co-Authors: P J Albert, J J B Smith, B K Mitchell, A T WhiteheadAbstract:Abstract 1. 1. Electrophysiological responses to NaCl, sucrose, and a complex mixture containing NaCl, glucose, fructose, phenylalanine and valine were obtained from large, medium, and small hair sensilla on the proboscis of the fleshfly Sarcophaga bullata. 2. 2. Responses from up to three cells in each sensillum were analysed and compared across the three types of sensilla. 3. 3. We found qualitative differences in the patterns of responses from the different hair types.
Reinhard Lakesharlan - One of the best experts on this subject based on the ideXlab platform.
-
morphology and physiology of the prosternal chordotonal organ of the sarcophagid fly Sarcophaga bullata parker
Journal of Insect Physiology, 2007Co-Authors: Heiko Stolting, Andreas Stumpner, Reinhard LakesharlanAbstract:The anatomy and the physiology of the prosternal chordotonal organ (pCO) within the prothorax of Sarcophaga bullata is analysed. Neuroanatomical studies illustrate that the approximately 35 sensory axons terminate within the median ventral association centre of the different neuromeres of the thoracico-abdominal ganglion. At the single-cell level two classes of receptor cells can be discriminated physiologically and morphologically: receptor cells with dorso-lateral branches in the mesothoracic neuromere are insensitive to frequencies below approximately 1 kHz. Receptor cells without such branches respond most sensitive at lower frequencies. Absolute thresholds vary between 0.2 and 8 m/s2 for different frequencies. The sensory information is transmitted to the brain via ascending interneurons. Functional analyses reveal a mechanical transmission of forced head rotations and of foreleg vibrations to the attachment site of the pCO. In summed action potential recordings a physiological correlate was found to stimuli with parameters of leg vibrations, rather than to those of head rotation. The data represent a first physiological study of a putative predecessor organ of an insect ear.
