The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Luca Rossi - One of the best experts on this subject based on the ideXlab platform.
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Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei
Parasites & Vectors, 2015Co-Authors: Samer Angelone-alasaad, Mario Pasquetti, Abdulaziz N. Alagaili, Federica Berrilli, Vincent Obanda, Mohamed A. Gebely, Ramón C. Soriguer, Annarita Molinar Min, Stefano D’amelio, Luca RossiAbstract:Background The mite Sarcoptes scabiei has a known host-range of over 100 mammal species including humans. One of the prime objectives of the Sarcoptes -World Molecular Network (WMN) is to design and develop universal Sarcoptes PCR-based diagnosis methods. Methods We describe here for the first time two universal mitochondrial-based diagnosis methods: (i) conventional end-point PCR and (ii) TaqMan real-time PCR. The design of both of these universal diagnosis methods was based on Sarcoptes samples collected from 23 host species in 14 countries. Results These methods, based on skin scrapings, were successfully used to etiologically confirm the diagnosis of different clinical degrees of sarcoptic mange in 48 animals belonging to six species. These universal PCR-based diagnosis methods are highly specific, technically sensitive and simple, and are based on the amplification of 135 bp from the Mitochondrial 16S rDNA. The method based on TaqMan real-time qPCR was more sensitive than the conventional end-point PCR. Conclusions Two universal PCR-based diagnosis methods for S. scabiei were successfully designed and applied; one based on conventional end-point PCR and the other on TaqMan real-time PCR. We recommend further testing and the application of these new universal methods worldwide.
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The neglected navigating web of the incomprehensibly emerging and re-emerging Sarcoptes mite.
Infection genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2013Co-Authors: Samer Alasaad, Jesús M. Pérez, Luca Rossi, Jorg Heukelbach, Omar Hamarsheh, Moses Otiende, Xing-quang ZhuAbstract:Parasite presence in any ecosystem generates complex navigating webs (Parasite-NW) within the system, through which parasites move from one to another host. The appropriate assimilation of parasite navigating web is pivotal for a better understanding of pathogen flow in the ecosystem, with implications for disease control. Sarcoptes mite has been approached from medical, veterinary, entomological, physiological and, recently, molecular sides, to understand its epidemiological navigating web between isolates from different hosts and geographical regions. The obtained conclusions are still a matter of debate. Sarcoptes navigating web (Sarcoptes-NW) is intricate and uncertain, with unexplainable pathogenic flow. In this review we summarize by which routes, under what conditions and at what levels the Sarcoptes mite moves among its hosts.
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Genetic epidemiology of Sarcoptes scabiei in the Iberian wolf in Asturias, Spain.
Veterinary parasitology, 2013Co-Authors: Alvaro Oleaga, Ramón C. Soriguer, Samer Alasaad, Sandra Maione, Luca Rossi, Rosa Casais, Joaquin Vicente, Christian GortazarAbstract:During the last decades, attempts have been made to understand the molecular epidemiology of Sarcoptes scabiei, and to detect and clarify the differences between isolates from different hosts and geographic regions. Two main phenomena have been described: (i) host-taxon derived-Sarcoptes mite infection in European wild animals (revealing the presence of three separate clusters, namely herbivore-, carnivore- and omnivore-derived Sarcoptes populations in Europe) and (ii) prey-to-predator Sarcoptes mite infection in the Masai Mara ecosystem. Using one multiplex of 9 microsatellite markers and Sarcoptes mite samples from sympatric Pyrenean chamois, red deer, red fox and Iberian wolf, different population structure analyses revealed concordance with the host-taxon law described for wild animals in Europe, with two main host-derived Sarcoptes mite populations, herbivore- and carnivore-derived. Surprisingly, Iberian wolf derived Sarcoptes populations had the highest genetic diversity among the other populations, including two different subpopulations: one similar to the herbivore-derived Sarcoptes populations, and another similar to carnivore (fox)-derived Sarcoptes mite population. The host-taxon effect in wild animals is still supported with the maintenance of carnivore- and herbivore-derived Sarcoptes clusters' separation in analyzed mites. However, this phenomenon could be modified with the inclusion of a large predator as wolf in the present work, revealing prey-to-predator Sarcoptes mite infection between the studied host-taxa and suggesting the importance of wolf's immune system for explaining the high variability reported in C. lupus derived mites. Further studies of host diet, behavior and movement, and regarding the role played by its immune system, would be of great help to clarify interactions between the two hypotheses, host-taxon and prey-to-predator. Copyright © 2013 Elsevier B.V. All rights reserved.
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The curse of the prey: Sarcoptes mite molecular analysis reveals potential prey-to-predator parasitic infestation in wild animals from Masai Mara, Kenya
Parasites & vectors, 2011Co-Authors: Francis Gakuya, Ramón C. Soriguer, Luca Rossi, Jn Ombui, Ndichu Maingi, G. Muchemi, William O. Ogara, Samer AlasaadAbstract:Background Recently, there have been attempts to understand the molecular epidemiology of Sarcoptes scabiei, to evaluate the gene flow between isolates of S. scabiei from different hosts and geographic regions. However, to our knowledge, a molecular study has not been carried out to assess the molecular diversity and gene flow of Sarcoptes mite in a predator/prey ecosystem.
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temporal stability in the genetic structure of Sarcoptes scabiei under the host taxon law empirical evidences from wildlife derived Sarcoptes mite in asturias spain
Parasites & Vectors, 2011Co-Authors: Annarita Molinar Min, Ramón C. Soriguer, Samer Alasaad, Luca Rossi, Alvaro Oleaga, Rosa Casais, Christian GortazarAbstract:Background Implicitly, parasite molecular studies assume temporal genetic stability. In this study we tested, for the first time to our knowledge, the extent of changes in genetic diversity and structure of Sarcoptes mite populations from Pyrenean chamois (Rupicapra pyrenaica) in Asturias (Spain), using one multiplex of 9 microsatellite markers and Sarcoptes samples from sympatric Pyrenean chamois, red deer (Cervus elaphus), roe deer (Capreolus capreolus) and red fox (Vulpes vulpes).
Ramón C. Soriguer - One of the best experts on this subject based on the ideXlab platform.
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Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei
Parasites & vectors, 2015Co-Authors: Samer Angelone-alasaad, Annarita Molinar Min, Mario Pasquetti, Abdulaziz N. Alagaili, Stefano D'amelio, Federica Berrilli, Vincent Obanda, Mohamed A. Gebely, Ramón C. Soriguer, L. RossiAbstract:Background The mite Sarcoptes scabiei has a known host-range of over 100 mammal species including humans. One of the prime objectives of the Sarcoptes-World Molecular Network (WMN) is to design and develop universal Sarcoptes PCR-based diagnosis methods.
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Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei
Parasites & Vectors, 2015Co-Authors: Samer Angelone-alasaad, Mario Pasquetti, Abdulaziz N. Alagaili, Federica Berrilli, Vincent Obanda, Mohamed A. Gebely, Ramón C. Soriguer, Annarita Molinar Min, Stefano D’amelio, Luca RossiAbstract:Background The mite Sarcoptes scabiei has a known host-range of over 100 mammal species including humans. One of the prime objectives of the Sarcoptes -World Molecular Network (WMN) is to design and develop universal Sarcoptes PCR-based diagnosis methods. Methods We describe here for the first time two universal mitochondrial-based diagnosis methods: (i) conventional end-point PCR and (ii) TaqMan real-time PCR. The design of both of these universal diagnosis methods was based on Sarcoptes samples collected from 23 host species in 14 countries. Results These methods, based on skin scrapings, were successfully used to etiologically confirm the diagnosis of different clinical degrees of sarcoptic mange in 48 animals belonging to six species. These universal PCR-based diagnosis methods are highly specific, technically sensitive and simple, and are based on the amplification of 135 bp from the Mitochondrial 16S rDNA. The method based on TaqMan real-time qPCR was more sensitive than the conventional end-point PCR. Conclusions Two universal PCR-based diagnosis methods for S. scabiei were successfully designed and applied; one based on conventional end-point PCR and the other on TaqMan real-time PCR. We recommend further testing and the application of these new universal methods worldwide.
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genetic epidemiology of Sarcoptes scabiei in the iberian wolf in asturias spain
Veterinary Parasitology, 2013Co-Authors: Alvaro Oleaga, Ramón C. Soriguer, L. Rossi, Samer Alasaad, Sandra Maione, Rosa Casais, Joaquin Vicente, Christian GortazarAbstract:Abstract Background During the last decades, attempts have been made to understand the molecular epidemiology of Sarcoptes scabiei , and to detect and clarify the differences between isolates from different hosts and geographic regions. Two main phenomena have been described: (i) host-taxon derived- Sarcoptes mite infection in European wild animals (revealing the presence of three separate clusters, namely herbivore-, carnivore- and omnivore-derived Sarcoptes populations in Europe) and (ii) prey-to-predator Sarcoptes mite infection in the Masai Mara ecosystem. Results Using one multiplex of 9 microsatellite markers and Sarcoptes mite samples from sympatric Pyrenean chamois, red deer, red fox and Iberian wolf, different population structure analyses revealed concordance with the host-taxon law described for wild animals in Europe, with two main host-derived Sarcoptes mite populations, herbivore- and carnivore-derived. Surprisingly, Iberian wolf derived Sarcoptes populations had the highest genetic diversity among the other populations, including two different subpopulations: one similar to the herbivore-derived Sarcoptes populations, and another similar to carnivore (fox)-derived Sarcoptes mite population. Conclusions The host-taxon effect in wild animals is still supported with the maintenance of carnivore- and herbivore-derived Sarcoptes clusters’ separation in analyzed mites. However, this phenomenon could be modified with the inclusion of a large predator as wolf in the present work, revealing prey-to-predator Sarcoptes mite infection between the studied host-taxa and suggesting the importance of wolf's immune system for explaining the high variability reported in C. lupus derived mites. Further studies of host diet, behavior and movement, and regarding the role played by its immune system, would be of great help to clarify interactions between the two hypotheses, host-taxon and prey-to-predator.
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Genetic epidemiology of Sarcoptes scabiei in the Iberian wolf in Asturias, Spain.
Veterinary parasitology, 2013Co-Authors: Alvaro Oleaga, Ramón C. Soriguer, Samer Alasaad, Sandra Maione, Luca Rossi, Rosa Casais, Joaquin Vicente, Christian GortazarAbstract:During the last decades, attempts have been made to understand the molecular epidemiology of Sarcoptes scabiei, and to detect and clarify the differences between isolates from different hosts and geographic regions. Two main phenomena have been described: (i) host-taxon derived-Sarcoptes mite infection in European wild animals (revealing the presence of three separate clusters, namely herbivore-, carnivore- and omnivore-derived Sarcoptes populations in Europe) and (ii) prey-to-predator Sarcoptes mite infection in the Masai Mara ecosystem. Using one multiplex of 9 microsatellite markers and Sarcoptes mite samples from sympatric Pyrenean chamois, red deer, red fox and Iberian wolf, different population structure analyses revealed concordance with the host-taxon law described for wild animals in Europe, with two main host-derived Sarcoptes mite populations, herbivore- and carnivore-derived. Surprisingly, Iberian wolf derived Sarcoptes populations had the highest genetic diversity among the other populations, including two different subpopulations: one similar to the herbivore-derived Sarcoptes populations, and another similar to carnivore (fox)-derived Sarcoptes mite population. The host-taxon effect in wild animals is still supported with the maintenance of carnivore- and herbivore-derived Sarcoptes clusters' separation in analyzed mites. However, this phenomenon could be modified with the inclusion of a large predator as wolf in the present work, revealing prey-to-predator Sarcoptes mite infection between the studied host-taxa and suggesting the importance of wolf's immune system for explaining the high variability reported in C. lupus derived mites. Further studies of host diet, behavior and movement, and regarding the role played by its immune system, would be of great help to clarify interactions between the two hypotheses, host-taxon and prey-to-predator. Copyright © 2013 Elsevier B.V. All rights reserved.
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The curse of the prey: Sarcoptes mite molecular analysis reveals potential prey-to-predator parasitic infestation in wild animals from Masai Mara, Kenya
Parasites & vectors, 2011Co-Authors: Francis Gakuya, Ramón C. Soriguer, Luca Rossi, Jn Ombui, Ndichu Maingi, G. Muchemi, William O. Ogara, Samer AlasaadAbstract:Background Recently, there have been attempts to understand the molecular epidemiology of Sarcoptes scabiei, to evaluate the gene flow between isolates of S. scabiei from different hosts and geographic regions. However, to our knowledge, a molecular study has not been carried out to assess the molecular diversity and gene flow of Sarcoptes mite in a predator/prey ecosystem.
Samer Angelone-alasaad - One of the best experts on this subject based on the ideXlab platform.
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Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei
Parasites & vectors, 2015Co-Authors: Samer Angelone-alasaad, Annarita Molinar Min, Mario Pasquetti, Abdulaziz N. Alagaili, Stefano D'amelio, Federica Berrilli, Vincent Obanda, Mohamed A. Gebely, Ramón C. Soriguer, L. RossiAbstract:Background The mite Sarcoptes scabiei has a known host-range of over 100 mammal species including humans. One of the prime objectives of the Sarcoptes-World Molecular Network (WMN) is to design and develop universal Sarcoptes PCR-based diagnosis methods.
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Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei
Parasites & Vectors, 2015Co-Authors: Samer Angelone-alasaad, Mario Pasquetti, Abdulaziz N. Alagaili, Federica Berrilli, Vincent Obanda, Mohamed A. Gebely, Ramón C. Soriguer, Annarita Molinar Min, Stefano D’amelio, Luca RossiAbstract:Background The mite Sarcoptes scabiei has a known host-range of over 100 mammal species including humans. One of the prime objectives of the Sarcoptes -World Molecular Network (WMN) is to design and develop universal Sarcoptes PCR-based diagnosis methods. Methods We describe here for the first time two universal mitochondrial-based diagnosis methods: (i) conventional end-point PCR and (ii) TaqMan real-time PCR. The design of both of these universal diagnosis methods was based on Sarcoptes samples collected from 23 host species in 14 countries. Results These methods, based on skin scrapings, were successfully used to etiologically confirm the diagnosis of different clinical degrees of sarcoptic mange in 48 animals belonging to six species. These universal PCR-based diagnosis methods are highly specific, technically sensitive and simple, and are based on the amplification of 135 bp from the Mitochondrial 16S rDNA. The method based on TaqMan real-time qPCR was more sensitive than the conventional end-point PCR. Conclusions Two universal PCR-based diagnosis methods for S. scabiei were successfully designed and applied; one based on conventional end-point PCR and the other on TaqMan real-time PCR. We recommend further testing and the application of these new universal methods worldwide.
Samer Alasaad - One of the best experts on this subject based on the ideXlab platform.
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genetic epidemiology of Sarcoptes scabiei in the iberian wolf in asturias spain
Veterinary Parasitology, 2013Co-Authors: Alvaro Oleaga, Ramón C. Soriguer, L. Rossi, Samer Alasaad, Sandra Maione, Rosa Casais, Joaquin Vicente, Christian GortazarAbstract:Abstract Background During the last decades, attempts have been made to understand the molecular epidemiology of Sarcoptes scabiei , and to detect and clarify the differences between isolates from different hosts and geographic regions. Two main phenomena have been described: (i) host-taxon derived- Sarcoptes mite infection in European wild animals (revealing the presence of three separate clusters, namely herbivore-, carnivore- and omnivore-derived Sarcoptes populations in Europe) and (ii) prey-to-predator Sarcoptes mite infection in the Masai Mara ecosystem. Results Using one multiplex of 9 microsatellite markers and Sarcoptes mite samples from sympatric Pyrenean chamois, red deer, red fox and Iberian wolf, different population structure analyses revealed concordance with the host-taxon law described for wild animals in Europe, with two main host-derived Sarcoptes mite populations, herbivore- and carnivore-derived. Surprisingly, Iberian wolf derived Sarcoptes populations had the highest genetic diversity among the other populations, including two different subpopulations: one similar to the herbivore-derived Sarcoptes populations, and another similar to carnivore (fox)-derived Sarcoptes mite population. Conclusions The host-taxon effect in wild animals is still supported with the maintenance of carnivore- and herbivore-derived Sarcoptes clusters’ separation in analyzed mites. However, this phenomenon could be modified with the inclusion of a large predator as wolf in the present work, revealing prey-to-predator Sarcoptes mite infection between the studied host-taxa and suggesting the importance of wolf's immune system for explaining the high variability reported in C. lupus derived mites. Further studies of host diet, behavior and movement, and regarding the role played by its immune system, would be of great help to clarify interactions between the two hypotheses, host-taxon and prey-to-predator.
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The neglected navigating web of the incomprehensibly emerging and re-emerging Sarcoptes mite.
Infection genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2013Co-Authors: Samer Alasaad, Jesús M. Pérez, Luca Rossi, Jorg Heukelbach, Omar Hamarsheh, Moses Otiende, Xing-quang ZhuAbstract:Parasite presence in any ecosystem generates complex navigating webs (Parasite-NW) within the system, through which parasites move from one to another host. The appropriate assimilation of parasite navigating web is pivotal for a better understanding of pathogen flow in the ecosystem, with implications for disease control. Sarcoptes mite has been approached from medical, veterinary, entomological, physiological and, recently, molecular sides, to understand its epidemiological navigating web between isolates from different hosts and geographical regions. The obtained conclusions are still a matter of debate. Sarcoptes navigating web (Sarcoptes-NW) is intricate and uncertain, with unexplainable pathogenic flow. In this review we summarize by which routes, under what conditions and at what levels the Sarcoptes mite moves among its hosts.
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Genetic epidemiology of Sarcoptes scabiei in the Iberian wolf in Asturias, Spain.
Veterinary parasitology, 2013Co-Authors: Alvaro Oleaga, Ramón C. Soriguer, Samer Alasaad, Sandra Maione, Luca Rossi, Rosa Casais, Joaquin Vicente, Christian GortazarAbstract:During the last decades, attempts have been made to understand the molecular epidemiology of Sarcoptes scabiei, and to detect and clarify the differences between isolates from different hosts and geographic regions. Two main phenomena have been described: (i) host-taxon derived-Sarcoptes mite infection in European wild animals (revealing the presence of three separate clusters, namely herbivore-, carnivore- and omnivore-derived Sarcoptes populations in Europe) and (ii) prey-to-predator Sarcoptes mite infection in the Masai Mara ecosystem. Using one multiplex of 9 microsatellite markers and Sarcoptes mite samples from sympatric Pyrenean chamois, red deer, red fox and Iberian wolf, different population structure analyses revealed concordance with the host-taxon law described for wild animals in Europe, with two main host-derived Sarcoptes mite populations, herbivore- and carnivore-derived. Surprisingly, Iberian wolf derived Sarcoptes populations had the highest genetic diversity among the other populations, including two different subpopulations: one similar to the herbivore-derived Sarcoptes populations, and another similar to carnivore (fox)-derived Sarcoptes mite population. The host-taxon effect in wild animals is still supported with the maintenance of carnivore- and herbivore-derived Sarcoptes clusters' separation in analyzed mites. However, this phenomenon could be modified with the inclusion of a large predator as wolf in the present work, revealing prey-to-predator Sarcoptes mite infection between the studied host-taxa and suggesting the importance of wolf's immune system for explaining the high variability reported in C. lupus derived mites. Further studies of host diet, behavior and movement, and regarding the role played by its immune system, would be of great help to clarify interactions between the two hypotheses, host-taxon and prey-to-predator. Copyright © 2013 Elsevier B.V. All rights reserved.
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The curse of the prey: Sarcoptes mite molecular analysis reveals potential prey-to-predator parasitic infestation in wild animals from Masai Mara, Kenya
Parasites & vectors, 2011Co-Authors: Francis Gakuya, Ramón C. Soriguer, Luca Rossi, Jn Ombui, Ndichu Maingi, G. Muchemi, William O. Ogara, Samer AlasaadAbstract:Background Recently, there have been attempts to understand the molecular epidemiology of Sarcoptes scabiei, to evaluate the gene flow between isolates of S. scabiei from different hosts and geographic regions. However, to our knowledge, a molecular study has not been carried out to assess the molecular diversity and gene flow of Sarcoptes mite in a predator/prey ecosystem.
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temporal stability in the genetic structure of Sarcoptes scabiei under the host taxon law empirical evidences from wildlife derived Sarcoptes mite in asturias spain
Parasites & Vectors, 2011Co-Authors: Annarita Molinar Min, Ramón C. Soriguer, Samer Alasaad, Luca Rossi, Alvaro Oleaga, Rosa Casais, Christian GortazarAbstract:Background Implicitly, parasite molecular studies assume temporal genetic stability. In this study we tested, for the first time to our knowledge, the extent of changes in genetic diversity and structure of Sarcoptes mite populations from Pyrenean chamois (Rupicapra pyrenaica) in Asturias (Spain), using one multiplex of 9 microsatellite markers and Sarcoptes samples from sympatric Pyrenean chamois, red deer (Cervus elaphus), roe deer (Capreolus capreolus) and red fox (Vulpes vulpes).
L. Rossi - One of the best experts on this subject based on the ideXlab platform.
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Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei
Parasites & vectors, 2015Co-Authors: Samer Angelone-alasaad, Annarita Molinar Min, Mario Pasquetti, Abdulaziz N. Alagaili, Stefano D'amelio, Federica Berrilli, Vincent Obanda, Mohamed A. Gebely, Ramón C. Soriguer, L. RossiAbstract:Background The mite Sarcoptes scabiei has a known host-range of over 100 mammal species including humans. One of the prime objectives of the Sarcoptes-World Molecular Network (WMN) is to design and develop universal Sarcoptes PCR-based diagnosis methods.
-
genetic epidemiology of Sarcoptes scabiei in the iberian wolf in asturias spain
Veterinary Parasitology, 2013Co-Authors: Alvaro Oleaga, Ramón C. Soriguer, L. Rossi, Samer Alasaad, Sandra Maione, Rosa Casais, Joaquin Vicente, Christian GortazarAbstract:Abstract Background During the last decades, attempts have been made to understand the molecular epidemiology of Sarcoptes scabiei , and to detect and clarify the differences between isolates from different hosts and geographic regions. Two main phenomena have been described: (i) host-taxon derived- Sarcoptes mite infection in European wild animals (revealing the presence of three separate clusters, namely herbivore-, carnivore- and omnivore-derived Sarcoptes populations in Europe) and (ii) prey-to-predator Sarcoptes mite infection in the Masai Mara ecosystem. Results Using one multiplex of 9 microsatellite markers and Sarcoptes mite samples from sympatric Pyrenean chamois, red deer, red fox and Iberian wolf, different population structure analyses revealed concordance with the host-taxon law described for wild animals in Europe, with two main host-derived Sarcoptes mite populations, herbivore- and carnivore-derived. Surprisingly, Iberian wolf derived Sarcoptes populations had the highest genetic diversity among the other populations, including two different subpopulations: one similar to the herbivore-derived Sarcoptes populations, and another similar to carnivore (fox)-derived Sarcoptes mite population. Conclusions The host-taxon effect in wild animals is still supported with the maintenance of carnivore- and herbivore-derived Sarcoptes clusters’ separation in analyzed mites. However, this phenomenon could be modified with the inclusion of a large predator as wolf in the present work, revealing prey-to-predator Sarcoptes mite infection between the studied host-taxa and suggesting the importance of wolf's immune system for explaining the high variability reported in C. lupus derived mites. Further studies of host diet, behavior and movement, and regarding the role played by its immune system, would be of great help to clarify interactions between the two hypotheses, host-taxon and prey-to-predator.
