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Saso Ivanovski - One of the best experts on this subject based on the ideXlab platform.
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Advanced tissue engineering Scaffold Design for regeneration of the complex hierarchical periodontal structure
Journal of Clinical Periodontology, 2014Co-Authors: Pedro F. Costa, Qiyi Zhang, Cedryck Vaquette, Saso Ivanovski, Rui L. Reis, Dietmar Werner HutmacherAbstract:AIM: This study investigated the ability of an osteoconductive biphasic Scaffold to simultaneously regenerate alveolar bone, periodontal ligament and cementum.\n\nMATERIALS AND METHODS: A biphasic Scaffold was built by attaching a fused deposition modelled bone compartment to a melt electrospun periodontal compartment. The bone compartment was coated with a calcium phosphate (CaP) layer for increasing osteoconductivity, seeded with osteoblasts and cultured in vitro for 6 weeks. The resulting constructs were then complemented with the placement of PDL cell sheets on the periodontal compartment, attached to a dentin block and subcutaneously implanted into athymic rats for 8 weeks. Scanning electron microscopy, X-ray diffraction, alkaline phosphatase and DNA content quantification, confocal laser microscopy, micro computerized tomography and histological analysis were employed to evaluate the Scaffold's performance.\n\nRESULTS: The in vitro study showed that alkaline phosphatase activity was significantly increased in the CaP-coated samples and they also displayed enhanced mineralization. In the in vivo study, significantly more bone formation was observed in the coated Scaffolds. Histological analysis revealed that the large pore size of the periodontal compartment permitted vascularization of the cell sheets, and periodontal attachment was achieved at the dentin interface.\n\nCONCLUSIONS: This work demonstrates that the combination of cell sheet technology together with an osteoconductive biphasic Scaffold could be utilized to address the limitations of current periodontal regeneration techniques.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Wei Fan, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:Abstract This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Institute of Health and Biomedical Innovation; Science & Engineering Faculty, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
Cedryck Vaquette - One of the best experts on this subject based on the ideXlab platform.
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Advanced tissue engineering Scaffold Design for regeneration of the complex hierarchical periodontal structure
Journal of Clinical Periodontology, 2014Co-Authors: Pedro F. Costa, Qiyi Zhang, Cedryck Vaquette, Saso Ivanovski, Rui L. Reis, Dietmar Werner HutmacherAbstract:AIM: This study investigated the ability of an osteoconductive biphasic Scaffold to simultaneously regenerate alveolar bone, periodontal ligament and cementum.\n\nMATERIALS AND METHODS: A biphasic Scaffold was built by attaching a fused deposition modelled bone compartment to a melt electrospun periodontal compartment. The bone compartment was coated with a calcium phosphate (CaP) layer for increasing osteoconductivity, seeded with osteoblasts and cultured in vitro for 6 weeks. The resulting constructs were then complemented with the placement of PDL cell sheets on the periodontal compartment, attached to a dentin block and subcutaneously implanted into athymic rats for 8 weeks. Scanning electron microscopy, X-ray diffraction, alkaline phosphatase and DNA content quantification, confocal laser microscopy, micro computerized tomography and histological analysis were employed to evaluate the Scaffold's performance.\n\nRESULTS: The in vitro study showed that alkaline phosphatase activity was significantly increased in the CaP-coated samples and they also displayed enhanced mineralization. In the in vivo study, significantly more bone formation was observed in the coated Scaffolds. Histological analysis revealed that the large pore size of the periodontal compartment permitted vascularization of the cell sheets, and periodontal attachment was achieved at the dentin interface.\n\nCONCLUSIONS: This work demonstrates that the combination of cell sheet technology together with an osteoconductive biphasic Scaffold could be utilized to address the limitations of current periodontal regeneration techniques.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Wei Fan, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:Abstract This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Institute of Health and Biomedical Innovation; Science & Engineering Faculty, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
Dietmar W Hutmacher - One of the best experts on this subject based on the ideXlab platform.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:Abstract This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Wei Fan, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Institute of Health and Biomedical Innovation; Science & Engineering Faculty, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
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microrobotics and mems based fabrication techniques for Scaffold based tissue engineering
Macromolecular Bioscience, 2005Co-Authors: Han Zhang, Dietmar W Hutmacher, Franck Chollet, A N Poo, Etienne BurdetAbstract:Scaffold based tissue engineering strategies use cells, biomolecules and a Scaffold to promote the repair and regeneration of tissues. Although Scaffold-based tissue engineering approaches are being actively developed, most are still experimental, and it is not yet clear what defines an ideal Scaffold/cell construct. Solid free form fabrication (SFF) techniques can precisely control matrix architecture (size, shape, interconnectivity, branching, geometry and orientation). The SFF methods enable the fabrication of Scaffolds with various Designs and material compositions, thus providing a control of mechanical properties, biological effects and degradation kinetics. This paper reviews the application of micro-robotics and MEMS-based fabrication techniques for Scaffold Design and fabrication. It also presents a novel robotic technique to fabricate Scaffold/cell constructs for tissue engineering by the assembly of microscopic building blocks.
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Scaffold Design and fabrication technologies for engineering tissues state of the art and future perspectives
Journal of Biomaterials Science-polymer Edition, 2001Co-Authors: Dietmar W HutmacherAbstract:Today, tissue engineers are attempting to engineer virtually every human tissue. Potential tissue-engineered products include cartilage, bone, heart valves, nerves, muscle, bladder, liver, etc. Tissue engineering techniques generally require the use of a porous Scaffold, which serves as a threedimensional template for initial cell attachment and subsequent tissue formation both in vitro and in vivo. The Scaffold provides the necessary support for cells to attach, proliferate, and maintain their differentiated function. Its architecture defines the ultimate shape of the new grown soft or hard tissue. In the early days of tissue engineering, clinically established materials such as collagen and polyglycolide were primarily considered as the material of choice for Scaffolds. The challenge for more advanced Scaffold systems is to arrange cells/tissue in an appropriate 3D configuration and present molecular signals in an appropriate spatial and temporal fashion so that the individual cells will grow and form t...
Yin Xiao - One of the best experts on this subject based on the ideXlab platform.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Wei Fan, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:Abstract This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Institute of Health and Biomedical Innovation; Science & Engineering Faculty, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
Stephen Hamlet - One of the best experts on this subject based on the ideXlab platform.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Wei Fan, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Biomaterials, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:Abstract This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum.
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a biphasic Scaffold Design combined with cell sheet technology for simultaneous regeneration of alveolar bone periodontal ligament complex
Institute of Health and Biomedical Innovation; Science & Engineering Faculty, 2012Co-Authors: Cedryck Vaquette, Dietmar W Hutmacher, Yin Xiao, Stephen Hamlet, Saso IvanovskiAbstract:This study describes the Design of a biphasic Scaffold composed of a Fused Deposition Modeling Scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic Scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The Scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these Scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the Scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These Scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic Scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.
