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Jong Rak Choi - One of the best experts on this subject based on the ideXlab platform.

  • automated detection of malaria associated pseudoeosinophilia and abnormal wbc Scattergram by the sysmex xe 2100 hematology analyzer a clinical study with 1 801 patients and real time quantitative pcr analysis in vivax malaria endemic area
    American Journal of Tropical Medicine and Hygiene, 2010
    Co-Authors: Jaewoo Song, Tae Sung Park, Jong Rak Choi
    Abstract:

    Recently, the XE-2100 hematology analyzer was investigated in a rather small patient group; pseudoeosinophilia or abnormal white blood cell (WBC) Scattergrams reported by this instrument were considered as significantly valuable diagnostic parameters in detecting vivax malaria. This study was conducted not only to assess the usefulness of pseudoeosinophilia or abnormal WBC Scattergram in vivax malaria-endemic areas with large patient groups (N = 1,801) but also to investigate the correlation of parasitemia and platelet count with pseudoeosinophilia and abnormal WBC Scattergrams. Of the 1,801 analyzed patients, 413 (22.9%) were found to have malaria by Wright–Giemsa stained blood smears. Overall, either pseudoeosinophilia or abnormal WBC Scattergram was detected in 191 of 413 malaria patients and 4 of 1,388 patients without malaria (sensitivity = 46.2%, specificity = 99.7%). We suggest that clinical hematology laboratories using the XE-2100 analyzer should be aware of such specific parameters, even with the absence of a clinical request.

  • Automated detection of malaria-associated pseudoeosinophilia and abnormal WBC Scattergram by the Sysmex XE-2100 hematology analyzer: a clinical study with 1,801 patients and real-time quantitative PCR analysis in vivax malaria-endemic area.
    The American journal of tropical medicine and hygiene, 2010
    Co-Authors: Jongha Yoo, Jaewoo Song, Tae Sung Park, Kyung A. Lee, Young-kyu Sun, Young Ah Kim, Jong Rak Choi
    Abstract:

    Recently, the XE-2100 hematology analyzer was investigated in a rather small patient group; pseudoeosinophilia or abnormal white blood cell (WBC) Scattergrams reported by this instrument were considered as significantly valuable diagnostic parameters in detecting vivax malaria. This study was conducted not only to assess the usefulness of pseudoeosinophilia or abnormal WBC Scattergram in vivax malaria-endemic areas with large patient groups (N = 1,801) but also to investigate the correlation of parasitemia and platelet count with pseudoeosinophilia and abnormal WBC Scattergrams. Of the 1,801 analyzed patients, 413 (22.9%) were found to have malaria by Wright–Giemsa stained blood smears. Overall, either pseudoeosinophilia or abnormal WBC Scattergram was detected in 191 of 413 malaria patients and 4 of 1,388 patients without malaria (sensitivity = 46.2%, specificity = 99.7%). We suggest that clinical hematology laboratories using the XE-2100 analyzer should be aware of such specific parameters, even with the absence of a clinical request.

Jaewoo Song - One of the best experts on this subject based on the ideXlab platform.

  • automated detection of malaria associated pseudoeosinophilia and abnormal wbc Scattergram by the sysmex xe 2100 hematology analyzer a clinical study with 1 801 patients and real time quantitative pcr analysis in vivax malaria endemic area
    American Journal of Tropical Medicine and Hygiene, 2010
    Co-Authors: Jaewoo Song, Tae Sung Park, Jong Rak Choi
    Abstract:

    Recently, the XE-2100 hematology analyzer was investigated in a rather small patient group; pseudoeosinophilia or abnormal white blood cell (WBC) Scattergrams reported by this instrument were considered as significantly valuable diagnostic parameters in detecting vivax malaria. This study was conducted not only to assess the usefulness of pseudoeosinophilia or abnormal WBC Scattergram in vivax malaria-endemic areas with large patient groups (N = 1,801) but also to investigate the correlation of parasitemia and platelet count with pseudoeosinophilia and abnormal WBC Scattergrams. Of the 1,801 analyzed patients, 413 (22.9%) were found to have malaria by Wright–Giemsa stained blood smears. Overall, either pseudoeosinophilia or abnormal WBC Scattergram was detected in 191 of 413 malaria patients and 4 of 1,388 patients without malaria (sensitivity = 46.2%, specificity = 99.7%). We suggest that clinical hematology laboratories using the XE-2100 analyzer should be aware of such specific parameters, even with the absence of a clinical request.

  • Automated detection of malaria-associated pseudoeosinophilia and abnormal WBC Scattergram by the Sysmex XE-2100 hematology analyzer: a clinical study with 1,801 patients and real-time quantitative PCR analysis in vivax malaria-endemic area.
    The American journal of tropical medicine and hygiene, 2010
    Co-Authors: Jongha Yoo, Jaewoo Song, Tae Sung Park, Kyung A. Lee, Young-kyu Sun, Young Ah Kim, Jong Rak Choi
    Abstract:

    Recently, the XE-2100 hematology analyzer was investigated in a rather small patient group; pseudoeosinophilia or abnormal white blood cell (WBC) Scattergrams reported by this instrument were considered as significantly valuable diagnostic parameters in detecting vivax malaria. This study was conducted not only to assess the usefulness of pseudoeosinophilia or abnormal WBC Scattergram in vivax malaria-endemic areas with large patient groups (N = 1,801) but also to investigate the correlation of parasitemia and platelet count with pseudoeosinophilia and abnormal WBC Scattergrams. Of the 1,801 analyzed patients, 413 (22.9%) were found to have malaria by Wright–Giemsa stained blood smears. Overall, either pseudoeosinophilia or abnormal WBC Scattergram was detected in 191 of 413 malaria patients and 4 of 1,388 patients without malaria (sensitivity = 46.2%, specificity = 99.7%). We suggest that clinical hematology laboratories using the XE-2100 analyzer should be aware of such specific parameters, even with the absence of a clinical request.

Takashi Morikawa - One of the best experts on this subject based on the ideXlab platform.

  • Investigation of morphological changes for the discrimination of nucleated red blood cells and other leukocytes in Sysmex XN hematology analyzer Scattergrams using transmission electron microscopy.
    Practical laboratory medicine, 2017
    Co-Authors: Masako Kaido, Yuri Takagi, Mari Kono, Atsushi Wada, Fumie Nakazawa, Shiori Yamamoto, Takashi Morikawa
    Abstract:

    Abstract Background The WNR channel of the XN-Series automated hematology analyzer (Sysmex) counts white blood cells (WBCs) and simultaneously performs a differential counting of basophils and nucleated red blood cells (NRBCs). The detection process involves exposing the cells to WNR-specific reagents containing an acidic detergent and a fluorescent dye and measuring the intensity of the forward scattered light (FSC) and side fluorescence light (SFL). Method We treated isolated peripheral WBCs and NRBCs with specific reagents and assessed the morphological changes in NRBCs and each leukocyte type using transmission electron microscopy (TEM). Results The results from a flow cytometer (FCM) showed that, after exposure to the reagents, basophils appeared on the highest FSC and SFL areas compared to other leukocytes on the WNR Scattergram. Owing to the hemolysis of reticulocytes and erythrocytes, NRBCs that survived the reagent treatment could be distinguished by their lower intensity than those of the other leukocytes on the WNR Scattergram. We investigated the significance of the relationship between the TEM and FCM results after the reagent treatment. Conclusion We confirmed that the WNR channel differentiates the blood cells on the WNR Scattergram based on differences in the amount of residual cytoplasm and nucleic acids.

  • Investigation of morphological changes for the discrimination of nucleated red blood cells and other leukocytes in Sysmex XN hematology analyzer Scattergrams using transmission electron microscopy
    Elsevier, 2017
    Co-Authors: Masako Kaido, Yuri Takagi, Mari Kono, Atsushi Wada, Fumie Nakazawa, Shiori Yamamoto, Takashi Morikawa
    Abstract:

    Background: The WNR channel of the XN-Series automated hematology analyzer (Sysmex) counts white blood cells (WBCs) and simultaneously performs a differential counting of basophils and nucleated red blood cells (NRBCs). The detection process involves exposing the cells to WNR-specific reagents containing an acidic detergent and a fluorescent dye and measuring the intensity of the forward scattered light (FSC) and side fluorescence light (SFL). Method: We treated isolated peripheral WBCs and NRBCs with specific reagents and assessed the morphological changes in NRBCs and each leukocyte type using transmission electron microscopy (TEM). Results: The results from a flow cytometer (FCM) showed that, after exposure to the reagents, basophils appeared on the highest FSC and SFL areas compared to other leukocytes on the WNR Scattergram. Owing to the hemolysis of reticulocytes and erythrocytes, NRBCs that survived the reagent treatment could be distinguished by their lower intensity than those of the other leukocytes on the WNR Scattergram. We investigated the significance of the relationship between the TEM and FCM results after the reagent treatment. Conclusion: We confirmed that the WNR channel differentiates the blood cells on the WNR Scattergram based on differences in the amount of residual cytoplasm and nucleic acids. Keywords: Automated hematology analyzer, Flow cytometry, Transmission electron microscopy, Leukocytes, NRBCs, Scatter light intensity, Fluorescent intensit

  • Comparison of optical data from flow cytometry and microscopy of leukocytes after exposure to specific reagents.
    Microscopy (Oxford England), 2015
    Co-Authors: Yuri Takagi, Mari Kono, Atsushi Wada, Shiori Yamamoto, Takashi Morikawa
    Abstract:

    Flow cytometry and microscopy are equally important in cell analysis. However, few reports have compared the optical data (cell size, internal complexity and fluorescent signal) from flow cytometry and microscopy. In this study, we compared the Scattergram from XN-series, a flow cytometry based hematology analyzer with microscopic images of similarly treated leukocytes, and investigated the correlation between the appearance in the Scattergram and cell size, internal complexity and fluorescence intensity. Healthy human peripheral blood was analyzed using the XN analyzer. For microscopic comparison, five types of leukocytes (monocytes, lymphocytes, basophils, neutrophils and eosinophils) were isolated from the peripheral blood by centrifugation and magnetic cell sorting, treated with a specific reagent and analyzed using electron microscopy, laser microscopy and confocal laser microscopy. Cell size, residual internal structures and fluorescence intensity correlated with intensity of forward-scattering, side scattering and fluorescent light. In this study, optical data from a clinically used hematology analyzer was clarified using microscopic images.

  • Comparison of the Leukocyte differentiation Scattergrams Between the XN-Series and the XE-Series of Hematology Analyzers
    2014
    Co-Authors: Sawako Kawauchi, Yuri Takagi, Mari Kono, Atsushi Wada, Takashi Morikawa
    Abstract:

    The newly launched XN-Series multiparameter, automated, hematology analyzer features a new channel named the WDF channel. Like the DIFF channel of the XE-Series, this channel can differentiate leukocytes from cells treated with specific reagents containing detergents and fluorescent stains, by using the 2-parameter flowcytometric method. The Scattergrams of the 2 channels have different patterns due to the differences in the reagents used as well as differences in the hardware and software. In particular, the WDF channel differentiates between lymphocytes and monocytes and enhances the separation capacity, thereby distinguishing it from the DIFF channel. In this study, we morphologically examined the reasons for the positional appearance of each subtype of the leukocytes on the Scattergram. Additionally, we also assessed the reason why lymphocytes and monocytes separated evidently on the WDF Scattergram, in terms of the influence of the reagents. First, using the XN- and XE-analyzers, we confirmed that the lymphocytes and monocytes separated better on the WDF Scattergram than on the DIFF Scattergram. Next, the separation of leukocytes was assessed following treatment with the WDF or DIFF reagents by the same method using the analyzers. Fluorescence staining was performed, and the intensity of the stained area in the leukocytes was observed under the Confocal Laser Scanning Microscope (CLSM) ; the intracellular structure of the leukocytes was observed under the Transmission Electron Microscope (TEM) ; and the size and surface structures of the leukocytes were observed under the Scanning Electron Microscope (SEM) . Each leukocyte appeared at the same position as they are measured in whole blood. Analysis under the CLSM showed that of all the leukocytes, the staining intensity after treatment with the reagents was highest in the monocytes, followed by that in the T lymphocytes, B lymphocytes, neutrophils, and eosinophils, which correlated with the side fluorescence intensity on the WDF Scattergram. In addition, observation by TEM revealed that of all the subtypes of leukocytes, the intracellular complexity after treatment with reagents was simplest in the lymphocytes, followed by that in monocytes, neutrophils, and eosinophils, which correlated with the side-scattered intensity on both the Scattergrams. Moreover, observation by SEM demonstrated that after treatment with reagents, the size of the lymphocytes was the smallest, followed by that of monocytes, neutrophils, and eosinophils, which correlated with the forwardscattered intensity on the WDF (FSC and SSC) Scattergram. These observations indicated that each leukocyte cluster was different in terms of the amount of its organelles as well as the detergent tolerance of cell membranes. From each electron microscope observation, it was clear that the intracellular structures of the leukocytes were retained after treatment with WDF reagents compared with that after treatment with DIFF reagents. In conclusion, the separation of lymphocytes and monocytes was better demonstrated by a WDF Scattergram than by a DIFF Scattergram.

Tae Sung Park - One of the best experts on this subject based on the ideXlab platform.

  • automated detection of malaria associated pseudoeosinophilia and abnormal wbc Scattergram by the sysmex xe 2100 hematology analyzer a clinical study with 1 801 patients and real time quantitative pcr analysis in vivax malaria endemic area
    American Journal of Tropical Medicine and Hygiene, 2010
    Co-Authors: Jaewoo Song, Tae Sung Park, Jong Rak Choi
    Abstract:

    Recently, the XE-2100 hematology analyzer was investigated in a rather small patient group; pseudoeosinophilia or abnormal white blood cell (WBC) Scattergrams reported by this instrument were considered as significantly valuable diagnostic parameters in detecting vivax malaria. This study was conducted not only to assess the usefulness of pseudoeosinophilia or abnormal WBC Scattergram in vivax malaria-endemic areas with large patient groups (N = 1,801) but also to investigate the correlation of parasitemia and platelet count with pseudoeosinophilia and abnormal WBC Scattergrams. Of the 1,801 analyzed patients, 413 (22.9%) were found to have malaria by Wright–Giemsa stained blood smears. Overall, either pseudoeosinophilia or abnormal WBC Scattergram was detected in 191 of 413 malaria patients and 4 of 1,388 patients without malaria (sensitivity = 46.2%, specificity = 99.7%). We suggest that clinical hematology laboratories using the XE-2100 analyzer should be aware of such specific parameters, even with the absence of a clinical request.

  • Automated detection of malaria-associated pseudoeosinophilia and abnormal WBC Scattergram by the Sysmex XE-2100 hematology analyzer: a clinical study with 1,801 patients and real-time quantitative PCR analysis in vivax malaria-endemic area.
    The American journal of tropical medicine and hygiene, 2010
    Co-Authors: Jongha Yoo, Jaewoo Song, Tae Sung Park, Kyung A. Lee, Young-kyu Sun, Young Ah Kim, Jong Rak Choi
    Abstract:

    Recently, the XE-2100 hematology analyzer was investigated in a rather small patient group; pseudoeosinophilia or abnormal white blood cell (WBC) Scattergrams reported by this instrument were considered as significantly valuable diagnostic parameters in detecting vivax malaria. This study was conducted not only to assess the usefulness of pseudoeosinophilia or abnormal WBC Scattergram in vivax malaria-endemic areas with large patient groups (N = 1,801) but also to investigate the correlation of parasitemia and platelet count with pseudoeosinophilia and abnormal WBC Scattergrams. Of the 1,801 analyzed patients, 413 (22.9%) were found to have malaria by Wright–Giemsa stained blood smears. Overall, either pseudoeosinophilia or abnormal WBC Scattergram was detected in 191 of 413 malaria patients and 4 of 1,388 patients without malaria (sensitivity = 46.2%, specificity = 99.7%). We suggest that clinical hematology laboratories using the XE-2100 analyzer should be aware of such specific parameters, even with the absence of a clinical request.

Wen Bin Wei - One of the best experts on this subject based on the ideXlab platform.

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