The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform
Yuehsheng Chen - One of the best experts on this subject based on the ideXlab platform.
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preventive activities of Scutellariae Radix gardeniae fructus and probiotics in salmonella enterica serovar typhimurium infection in chickens
Animal Feed Science and Technology, 2016Co-Authors: Yuanman Hsu, Chihsian Tseng, C H Chang, Daniel S Chen, Yuehsheng ChenAbstract:Abstract In this study, Scutellariae Radix (SR), Gardeniae fructus (GF), and probiotic-lactic acid bacteria (LAB) were evaluated to determine their efficiency in preventing an infection from Salmonella enterica serovar Typhimurium in chickens. Chickens were fed with a diet supplied with either LAB (0.5% of diet), SR (0.5% of diet), SR with LAB (0.5% of diet each), GF (0.5% of diet), GF with probiotics (0.5% of diet each), or a basal diet as a control for 10 days before being exposed to a 10 10 CFU dose of ST315. Clinical signs were monitored for 6 days post-challenge. The minimal dosage of S . Typhimurium strain ST315 used to infect chickens without causing serious sickness is 10 10 CFU/ml. The maximum amount of bacteria that was shed in feces was achieved 3 days after the initial infection. Herbs and LAB supplements reduced Salmonella fecal shedding and the bacterial load in intestinal tissues ( P P P
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application of Scutellariae Radix gardeniae fructus and probiotics to prevent salmonella enterica serovar choleraesuis infection in swine
Evidence-based Complementary and Alternative Medicine, 2013Co-Authors: Chiunghung Chang, Daniel S Chen, Yuehsheng Chen, Mingtang Chiou, Chinen Tsai, Yuanman HsuAbstract:Salmonella enterica serovar Choleraesuis, a host-adapted pathogen of swine, usually causes septicemia. Lactic acid bacteria (LAB) strains have been widely studied in recent years for their probiotic properties. In this study, a mouse infection model first screened for potential agents against infection, then a pig infection model evaluated effects of LAB strains and herbal plants against infection. Scutellariae Radix (SR) and Gardeniae fructus (GF) showed abilities to reduce bacteria shedding and suppressing serum level of TNF-α induced by infection in swine. Bioactivities of SR and GF were enhanced by combining with LAB strains, which alone could speed up the bacteria elimination time in feces and boost immunity of infected pigs. Baicalein and genipin exhibited stronger cytotoxicity than baicalin and geniposide did, as well as prevent Salmonella from invading macrophages. Our study suggests LAB strains as exhibiting multiple functions: preventing infection, enhancing immunity to prepare host defenses against further infection, and adjusting intestinal microbes' enzymatic activity in order to convert herbal compounds to active compounds. The SR/GF-LAB strain mixture holds potential infection-prevention agents supplied as feed additives.
Zhong Zuo - One of the best experts on this subject based on the ideXlab platform.
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brain uptake of bioactive flavones in Scutellariae Radix and its relationship to anxiolytic effect in mice
Molecular Pharmaceutics, 2017Co-Authors: Sophia Yui Kau Fong, Yin Cheong Wong, Qian Wang, Hong Xue, Zhong ZuoAbstract:Scutellariae Radix (SR) and its bioactive flavones elicit a variety of effects in the brain. However, the brain uptake of individual SR flavones and its relationship to the elicited effects after SR administration remain unknown. Moreover, previous studies seldom measured pharmacokinetic and pharmacodynamic outcomes simultaneously. In the current study, the brain uptake of six major SR flavones and the anxiolytic behavior following oral administration of a SR extract at two clinically relevant doses (600 and 1200 mg/kg twice daily) were simultaneously investigated in mice (n = 18 per group). Brain and plasma concentrations of the flavones were measured by LC–MS/MS, while the anxiolytic effect was evaluated using the elevated plus maze. To further investigate the mechanism behind the differential brain uptake of the six SR flavones, these flavones were separately administered to mice at an equivalent molar oral dose (n = 6). The brain tissue bindings of the SR flavones were also measured with the in vitro ...
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herb drug interactions between Scutellariae Radix and mefenamic acid simultaneous investigation of pharmacokinetics anti inflammatory effect and gastric damage in rats
Journal of Ethnopharmacology, 2015Co-Authors: Sophia Yui Kau Fong, Yin Cheong Wong, Chen Xie, Zhong ZuoAbstract:Abstract Ethnopharmacological relevance Scutellariae Radix (SR), the dried root of Scutellariae baicalensis Georgi, has a lot in common with non-steroidal anti-inflammatory drugs (NSAIDs). Their similarities in therapeutic action (anti-inflammation) and metabolic pathways (phase II metabolisms) may lead to co-administration by patients with the potential of pharmacokinetic and/or pharmacodynamic interactions. The current study aims to investigate the potential interactions between SR and an NSAID, mefenamic acid (MEF), on the overall pharmacokinetic dispositions, anti-inflammatory effects and adverse effects in rats. Materials and methods The current study simultaneously monitored the pharmacokinetic and pharmacodynamic interactions in a single animal. Four groups of Sprague-Dawley rats ( n =7 each) received oral doses of a standardized SR extract (300 mg/kg, twice daily), MEF (40 mg/kg, daily), combination of SR extract and MEF, and vehicle control, respectively, for 5 days. On Day 5, blood samples were collected after first dose over 24 h for the determination of (1) plasma concentrations of SR bioactive components, MEF and its metabolites by LC-MS/MS, and (2) prostaglandin E 2 (PGE 2 ) production and cyclooxygenase-2 (COX-2) gene expression by ex vivo analyses using LPS-stimulated RAW264.7 macrophage cells, ELISA and real time-PCR. After the rats were sacrificed, stomachs were isolated to assess their gross mucosal damage. Statistical comparisons were conducted using ANOVA and t -test. Results Minimal pharmacokinetic interaction between SR extract and MEF was observed. Co-administration of SR extract and MEF did not significantly alter the plasma concentration–time profile or the pharmacokinetic parameters such as C max , AUC 0→24 , T max or clearance. Pharmacodynamic interaction via the COX-2 pathway was observed. The PGE 2 level in LPS-stimulated RAW264.7 cells treated with plasma collected from control group over the 24 h sampling (AUC 0→24[PGE2] ) was 191981±8789 pg/ml hr, which was significantly reduced to 174,780±6531 and 46,225±1915 pg/ml hr by plasma collected from rats administered with SR extract and MEF, respectively. Co-administration of SR extract and MEF further potentiated the PGE 2 inhibition, with an AUC 0→24[PGE2] of 37013±2354 pg/ml hr ( p p p p Conclusions Co-administration of SR extract and MEF potentiated the anti-inflammatory effects, alleviated the MEF-induced stomach adverse effect while having minimal pharmacokinetic interactions. Our findings provide insight for combination therapy of SR extract and MEF against inflammatory diseases.
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development of a spe lc ms ms method for simultaneous quantification of baicalein wogonin oroxylin a and their glucuronides baicalin wogonoside and oroxyloside in rats and its application to brain uptake and plasma pharmacokinetic studies
Journal of Pharmaceutical and Biomedical Analysis, 2014Co-Authors: Sophia Yui Kau Fong, Yin Cheong Wong, Zhong ZuoAbstract:Abstract This study aims to identify and quantify the six major bioactive flavones of the traditional Chinese medicine Scutellariae Radix (RS), including baicalein, baicalin, wogonin, wogonoside, oroxylin A and oroxyloside in rat after oral administration of a standardized RS extract. A novel, sensitive and selective method for simultaneous determination of these six analytes in rat brain and plasma using solid phase extraction-liquid chromatography–tandem mass spectrometry (SPE-LC/MS/MS) was developed and fully validated. The lower limits of quantification (LLOQs) for the six RS flavones in brain tissue were 0.02 nmol/g. The LLOQs in plasma were 0.005 nmol/ml for B, W and OA, 0.025 nmol/ml for WG and OAG, and 0.1875 nmol/ml for BG. The current study provides novel evidence of the presence of all the tested RS flavones and an isoform of BG (BG′, probably baicalein-6-O-glucuronide) in the rat brain after oral administration of RS extract, suggesting their ability to permeate through the blood–brain barrier. The method was also successfully applied to the pharmacokinetic study of all these analytes in plasma after oral administration of RS extract (300 mg/kg) to Sprague-Dawley rats. The developed assay method provides a useful tool for both preclinical and clinical investigations on the disposition of RS flavones in brain and plasma.
Ing Jun Chen - One of the best experts on this subject based on the ideXlab platform.
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san huang xie xin tang reduces lipopolysaccharides induced hypotension and inflammatory mediators
Journal of Ethnopharmacology, 2005Co-Authors: Pei Ling Tsai, Yawbin Huang, Kuo Pyng Shen, Yi Hung Tsai, Yung Hsiung Lai, Ing Jun ChenAbstract:Abstract San-Huang-Xie-Xin-Tang (SHXT) is a traditional Chinese medicinal formula containing Coptidis rhizoma , Scutellariae Radix and Rhei rhizoma . The present study aimed to determine the preventive effects of standardized SHXT on lipopolysaccharides (LPS)-induced arterial hypotension, protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), cytokines formation and prostaglandin E 2 (PGE 2 ) production. LPS-induced activation of iNOS has been recognized to increase cytokines and nitric oxide, some of them play predominant roles in sepsis. Intravenous injection of LPS (10 mg/kg) caused a marked decrease of the mean arterial pressure in normotensive rats. However, the LPS-induced arterial hypotension was inhibited by SHXT (0.01 and 0.03 g/kg), when it was given 30 min before LPS. Moreover, plasma level of cytokines and PGE 2 were lowered by SHXT. In RAW 264.7 cells, SHXT (20–200 μg/ml) dose-dependently inhibited LPS (1 μg/ml)-induced iNOS and COX-2 expression, and it also significantly decreased LPS-induced cytokines in a dose-dependent manner. In conclusion, our data suggest that SHXT prevented LPS-induced arterial hypotension, which might be mediated through its inhibition activities on the expression of iNOS and COX-2, cytokines formation and PGE 2 production. Therefore, its protection activity against LPS-induced arterial hypotension and inflammatory mediators release might be beneficial in the treatment of endotoxin shock and/or associated inflammation.
Zhang Wang - One of the best experts on this subject based on the ideXlab platform.
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antistress effects of san huang xie xin decoction on restraint stressed mice revealed by 1h nmr based metabolomics and biochemistry analysis
Oxidative Medicine and Cellular Longevity, 2019Co-Authors: Huan Du, Ting-ting Kuang, Qing Zhang, Zhang WangAbstract:San-Huang-Xie-Xin decoction (SHXXD), composed of Rhei Radix et Rhizoma, Coptidis Rhizoma, and Scutellariae Radix, is a representative antipyretic and detoxifying prescription in traditional Chinese medicine. In this study, we investigated the antistress effects and underlying mechanisms of San-Huang-Xie-Xin decoction (SHXXD) on restraint-stressed mice by 1H NMR-based metabolomics combined with biochemistry assay. A total of 48 male mice (5 weeks old, 18-22 g) were divided randomly into 6 groups (), including the normal group, restraint-stressed group, vitamin C group (positive drug, 17 mg/kg), and 3-dosage groups of SHXXD (200, 400, and 800 mg/kg). The stress model was induced by restraining mice in a polypropylene centrifuge tube for 6 h every day. The rotarod test was performed, and several biochemical indicators were measured. Moreover, other 24 animals were divided into 3 groups () including the normal group, restraint-stressed group, and SHXXD group (800 mg/kg) for 1H NMR-based metabolomics analysis. Our results showed that SHXXD significantly increased the rotarod time, thymus index, spleen index, and the levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and interleukin- (IL-) 2, but decreased the levels of malondialdehyde (MDA), IL-1β, tumor necrosis factor- (TNF-) α, corticosterone (CORT), and adrenocorticotropic hormone (ACTH) in restraint-stressed mice. Moreover, the contents of eight endogenous metabolites that were changed by restraint stress were significantly reversed by SHXXD. The results of both metabolomics and biochemical analysis indicated that SHXXD (800 mg/kg, p.o.) could improve the biochemical changes and metabolic disorders in restraint-stressed mice by antioxidation and anti-inflammation, enhancing the body’s immune function and restoring several disturbed metabolic pathways (i.e., lipid metabolism, glycolysis and gluconeogenesis, inflammatory injury, and energy metabolism). Taken together, these results indicated that SHXXD has a potential antistress effect in restraint-stressed mice and could be considered as a candidate drug for stress-related disorders.
Yuanman Hsu - One of the best experts on this subject based on the ideXlab platform.
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preventive activities of Scutellariae Radix gardeniae fructus and probiotics in salmonella enterica serovar typhimurium infection in chickens
Animal Feed Science and Technology, 2016Co-Authors: Yuanman Hsu, Chihsian Tseng, C H Chang, Daniel S Chen, Yuehsheng ChenAbstract:Abstract In this study, Scutellariae Radix (SR), Gardeniae fructus (GF), and probiotic-lactic acid bacteria (LAB) were evaluated to determine their efficiency in preventing an infection from Salmonella enterica serovar Typhimurium in chickens. Chickens were fed with a diet supplied with either LAB (0.5% of diet), SR (0.5% of diet), SR with LAB (0.5% of diet each), GF (0.5% of diet), GF with probiotics (0.5% of diet each), or a basal diet as a control for 10 days before being exposed to a 10 10 CFU dose of ST315. Clinical signs were monitored for 6 days post-challenge. The minimal dosage of S . Typhimurium strain ST315 used to infect chickens without causing serious sickness is 10 10 CFU/ml. The maximum amount of bacteria that was shed in feces was achieved 3 days after the initial infection. Herbs and LAB supplements reduced Salmonella fecal shedding and the bacterial load in intestinal tissues ( P P P
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application of Scutellariae Radix gardeniae fructus and probiotics to prevent salmonella enterica serovar choleraesuis infection in swine
Evidence-based Complementary and Alternative Medicine, 2013Co-Authors: Chiunghung Chang, Daniel S Chen, Yuehsheng Chen, Mingtang Chiou, Chinen Tsai, Yuanman HsuAbstract:Salmonella enterica serovar Choleraesuis, a host-adapted pathogen of swine, usually causes septicemia. Lactic acid bacteria (LAB) strains have been widely studied in recent years for their probiotic properties. In this study, a mouse infection model first screened for potential agents against infection, then a pig infection model evaluated effects of LAB strains and herbal plants against infection. Scutellariae Radix (SR) and Gardeniae fructus (GF) showed abilities to reduce bacteria shedding and suppressing serum level of TNF-α induced by infection in swine. Bioactivities of SR and GF were enhanced by combining with LAB strains, which alone could speed up the bacteria elimination time in feces and boost immunity of infected pigs. Baicalein and genipin exhibited stronger cytotoxicity than baicalin and geniposide did, as well as prevent Salmonella from invading macrophages. Our study suggests LAB strains as exhibiting multiple functions: preventing infection, enhancing immunity to prepare host defenses against further infection, and adjusting intestinal microbes' enzymatic activity in order to convert herbal compounds to active compounds. The SR/GF-LAB strain mixture holds potential infection-prevention agents supplied as feed additives.
