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Gregory M L Patterson - One of the best experts on this subject based on the ideXlab platform.
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a potent novel anti hiv protein from the cultured cyanobacterium Scytonema varium
Biochemistry, 2003Co-Authors: Heidi R Bokesch, Gregory M L Patterson, Barry R Okeefe, Tawnya C Mckee, Lewis K Pannell, Roberta S Gardella, Raymond C Sowder, Jim A Turpin, Karen M Watson, Robert W BuckheitAbstract:A new anti-HIV protein, scytovirin, was isolated from aqueous extracts of the cultured cyanobacterium Scytonema varium. The protein displayed potent anticytopathic activity against laboratory strains and primary isolates of HIV-1 with EC50 values ranging from 0.3 to 22 nM. Scytovirin binds to viral coat proteins gp120, gp160, and gp41 but not to cellular receptor CD4 or other tested proteins. This unique protein consists of a single 95-amino acid chain with significant internal sequence duplication and 10 cysteines forming five intrachain disulfide bonds.
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Mirabimides A–D, new N-acylpyrrolinones from the blue-green alga Scytonema mirabile
Tetrahedron, 2001Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:Abstract Mirabimide A, the major imide from the terrestrial blue-green alga Scytonema mirabile (Dillwyn) Bornet (strain BY-8-1), is N-[2-(N,N-dimethylleucyl)oxy-3-methylpentanoyl-N-methylvalyl-prolyl]-4-methoxy-5-isopropyl-3-pyrrolin-2-one. Mirabimides B–D are the valyl, 2-oxy-3-methylbutanoyl, and N-acetyl-N-methylleucyl analogs, respectively. The absolute configurations of the five asymmetric carbons in the mirabimides are proposed to be all S , but the assignments for the dimethylleucine units in A–C and the hydroxyacid units in B–D are tentative.
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regulation of scytophycin accumulation in cultures of Scytonema ocellatum ii nutrient requirements
Applied Microbiology and Biotechnology, 1995Co-Authors: Gregory M L Patterson, Christine M BolisAbstract:The effects of optimal sources and concentrations of major nutrients (supplying N, S, P, K+, Na+, Ca2+, Mg2+, and inorganic carbon) and organic buffers on growth and secondary metabolite accumulation in Scytonema ocellatum strain FF-66-3 were determined. Nitrate, phosphate, magnesium, and sulfur had no specific stimulatory or inhibitory effects on scytophycin accumulation within the range of concentrations that supported optimal growth. Calcium concentrations greater than those required for growth (0.1 mM) stimulated scytophycin accumulation. Sodium carbonate concentrations in excess of 0.25 mM strongly inhibited growth. Ammonium (2.5 mM) inhibited both growth and product formation. 3-[N-Morpholino]propanesulfonic acid at 3–5 mM effectively controlled pH and facilitated both growth and product formation.
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regulation of scytophycin accumulation in cultures of Scytonema ocellatum i physical factors
Applied Microbiology and Biotechnology, 1993Co-Authors: Gregory M L Patterson, Christine M BolisAbstract:Scytonema ocellatum (Cyanobacteria or Cyanophyta) produces macrolide antibiotics of the scytophycin family that are antifungal, cytostatic agents and act by disrupting actin microfilaments. Scytophycin accumulation paralleled vegetative growth. Tolytoxin continued to accumulate throughout the growth cycle, whereas 6-hydroxy-7-O-methylscytophycin E (HMSE) and 19-O-demethylscytophycin C (DMSC) reached plateau levels prior to cessation of growth, suggesting a logical biosynthetic pathway of DMSC → ? → HMSE → tolytoxin. A rapid decrease in scytophycin content observed in newly inoculated cultures suggests that the scytophycins are continuously metabolized. The optimal temperature for production was 25°C. Continuous illumination at an intensity of at least 25 μml photons m−2 s−1 was required for maximum yield. Growth and metabolite production were optimal in the pH range of 8.0 to 8.5.
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mirabazoles minor tantazole related cytotoxins from the terrestrial blue green alga Scytonema mirabile
Tetrahedron Letters, 1991Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:Abstract Three tantazole-related compounds, viz. didehydromirabazole A ( 1 ) and mirabazoles B ( 2 ) and C ( 3 ), have been isolated as minor components from Scytonema mirabile (Dillwyn) Bornet (strain BY-8-1) and their structures elucidated.
Richard E Moore - One of the best experts on this subject based on the ideXlab platform.
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Mirabimides A–D, new N-acylpyrrolinones from the blue-green alga Scytonema mirabile
Tetrahedron, 2001Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:Abstract Mirabimide A, the major imide from the terrestrial blue-green alga Scytonema mirabile (Dillwyn) Bornet (strain BY-8-1), is N-[2-(N,N-dimethylleucyl)oxy-3-methylpentanoyl-N-methylvalyl-prolyl]-4-methoxy-5-isopropyl-3-pyrrolin-2-one. Mirabimides B–D are the valyl, 2-oxy-3-methylbutanoyl, and N-acetyl-N-methylleucyl analogs, respectively. The absolute configurations of the five asymmetric carbons in the mirabimides are proposed to be all S , but the assignments for the dimethylleucine units in A–C and the hydroxyacid units in B–D are tentative.
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synthesis and absolute configuration of an isotactic nonamethoxy 1 pentacosene from the blue green alga Scytonema ocellatum
Tetrahedron, 1994Co-Authors: Yuji Mori, Narumi Kawajiri, Hiroshi Furukawa, Richard E MooreAbstract:Abstract Synthesis of the novel isotactic nonamethoy-1-pentacosene (3) isolated from the terrestrial blue-green alga Scytonema ocellatum was accomplished in a stereocontrolled manner and the absolute configuration was established.
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mirabazoles minor tantazole related cytotoxins from the terrestrial blue green alga Scytonema mirabile
Tetrahedron Letters, 1991Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:Abstract Three tantazole-related compounds, viz. didehydromirabazole A ( 1 ) and mirabazoles B ( 2 ) and C ( 3 ), have been isolated as minor components from Scytonema mirabile (Dillwyn) Bornet (strain BY-8-1) and their structures elucidated.
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isotactic polymethoxy 1 alkenes from the terrestrial blue green alga Scytonema ocellatum structure and synthesis
Tetrahedron, 1991Co-Authors: Yuji Mori, Shmuel Carmeli, Richard E Moore, Makoto Suzuki, Yasunori Kohchi, Hirohide Noguchi, Gregory M L PattersonAbstract:Abstract Novel isotactic polymethoxy-1-alkenes 2 – 5 were isolated from the tolytoxin-producing blue-green alga Scytonema ocellatum . The gross structures and relative configurations were determined by NMR and mass spectral analysis. Synthesis of optically active 2 – 4 in a stereocontrolled manner established their absolute configurations.
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mirabimides a d new n acylpyrrolinones from the blue green alga Scytonema mirabile
Tetrahedron, 1991Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:Abstract Mirabimide A, the major imide from the terrestrial blue-green alga Scytonema mirabile (Dillwyn) Bornet (strain BY-8-1), is N-[2-(N,N-dimethylleucyl)oxy-3-methylpentanoyl-N-methylvalyl-prolyl]-4-methoxy-5-isopropyl-3-pyrrolin-2-one. Mirabimides B–D are the valyl, 2-oxy-3-methylbutanoyl, and N-acetyl-N-methylleucyl analogs, respectively. The absolute configurations of the five asymmetric carbons in the mirabimides are proposed to be all S , but the assignments for the dimethylleucine units in A–C and the hydroxyacid units in B–D are tentative.
Shmuel Carmeli - One of the best experts on this subject based on the ideXlab platform.
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Mirabimides A–D, new N-acylpyrrolinones from the blue-green alga Scytonema mirabile
Tetrahedron, 2001Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:Abstract Mirabimide A, the major imide from the terrestrial blue-green alga Scytonema mirabile (Dillwyn) Bornet (strain BY-8-1), is N-[2-(N,N-dimethylleucyl)oxy-3-methylpentanoyl-N-methylvalyl-prolyl]-4-methoxy-5-isopropyl-3-pyrrolin-2-one. Mirabimides B–D are the valyl, 2-oxy-3-methylbutanoyl, and N-acetyl-N-methylleucyl analogs, respectively. The absolute configurations of the five asymmetric carbons in the mirabimides are proposed to be all S , but the assignments for the dimethylleucine units in A–C and the hydroxyacid units in B–D are tentative.
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mirabazoles minor tantazole related cytotoxins from the terrestrial blue green alga Scytonema mirabile
Tetrahedron Letters, 1991Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:Abstract Three tantazole-related compounds, viz. didehydromirabazole A ( 1 ) and mirabazoles B ( 2 ) and C ( 3 ), have been isolated as minor components from Scytonema mirabile (Dillwyn) Bornet (strain BY-8-1) and their structures elucidated.
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isotactic polymethoxy 1 alkenes from the terrestrial blue green alga Scytonema ocellatum structure and synthesis
Tetrahedron, 1991Co-Authors: Yuji Mori, Shmuel Carmeli, Richard E Moore, Makoto Suzuki, Yasunori Kohchi, Hirohide Noguchi, Gregory M L PattersonAbstract:Abstract Novel isotactic polymethoxy-1-alkenes 2 – 5 were isolated from the tolytoxin-producing blue-green alga Scytonema ocellatum . The gross structures and relative configurations were determined by NMR and mass spectral analysis. Synthesis of optically active 2 – 4 in a stereocontrolled manner established their absolute configurations.
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mirabimides a d new n acylpyrrolinones from the blue green alga Scytonema mirabile
Tetrahedron, 1991Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:Abstract Mirabimide A, the major imide from the terrestrial blue-green alga Scytonema mirabile (Dillwyn) Bornet (strain BY-8-1), is N-[2-(N,N-dimethylleucyl)oxy-3-methylpentanoyl-N-methylvalyl-prolyl]-4-methoxy-5-isopropyl-3-pyrrolin-2-one. Mirabimides B–D are the valyl, 2-oxy-3-methylbutanoyl, and N-acetyl-N-methylleucyl analogs, respectively. The absolute configurations of the five asymmetric carbons in the mirabimides are proposed to be all S , but the assignments for the dimethylleucine units in A–C and the hydroxyacid units in B–D are tentative.
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tolytoxin and new scytophycins from three species of Scytonema
Journal of Natural Products, 1990Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L PattersonAbstract:: Tolytoxin [1], the major cytotoxin associated with Scytonema mirabile strain BY-8-1, Scytonema burmanicum strain DO-4-1 and Scytonema ocellatum strains DD-8-1, FF-65-1, and FF-66-3, has been shown to be 6-hydroxy-7-O-methylscytophycin B. Minor amounts of three other new cytotoxic scytophycins, 6-hydroxyscytophycin B [2], 19-O-demethylscytophycin C [3], and 6-hydroxy-7-O-methylscytophycin E [4], have also been isolated from these cyanophytes. The gross structures and stereochemistry are based on nmr and cd analysis and on comparison with scytophycins A-E.
Yuji Mori - One of the best experts on this subject based on the ideXlab platform.
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synthesis and absolute configuration of an isotactic nonamethoxy 1 pentacosene from the blue green alga Scytonema ocellatum
Tetrahedron, 1994Co-Authors: Yuji Mori, Narumi Kawajiri, Hiroshi Furukawa, Richard E MooreAbstract:Abstract Synthesis of the novel isotactic nonamethoy-1-pentacosene (3) isolated from the terrestrial blue-green alga Scytonema ocellatum was accomplished in a stereocontrolled manner and the absolute configuration was established.
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isotactic polymethoxy 1 alkenes from the terrestrial blue green alga Scytonema ocellatum structure and synthesis
Tetrahedron, 1991Co-Authors: Yuji Mori, Shmuel Carmeli, Richard E Moore, Makoto Suzuki, Yasunori Kohchi, Hirohide Noguchi, Gregory M L PattersonAbstract:Abstract Novel isotactic polymethoxy-1-alkenes 2 – 5 were isolated from the tolytoxin-producing blue-green alga Scytonema ocellatum . The gross structures and relative configurations were determined by NMR and mass spectral analysis. Synthesis of optically active 2 – 4 in a stereocontrolled manner established their absolute configurations.
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isonitriles from the blue green alga Scytonema mirabile
Journal of Organic Chemistry, 1990Co-Authors: Shmuel Carmeli, Richard E Moore, Gregory M L Patterson, Yuji Mori, Makoto SuzukiAbstract:Mise en evidence, chez une forme aerienne de l'algue Scytonema mirabile, de 6 nouveaux isonitriles faiblement cytotoxiques et antimicrobiens. Structure generale determinee par analyse spectrale; stereochimie resolue par degradation chimique et comparaison avec des composes synthetiques
Siba Prasad Adhikary - One of the best experts on this subject based on the ideXlab platform.
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estimation of growth and exopolysaccharide production by two soil cyanobacteria Scytonema tolypothrichoides and tolypothrix bouteillei as determined by cultivation in irradiance and temperature crossed gradients
Engineering in Life Sciences, 2019Co-Authors: Jana Kviderova, Dhanesh Kumar, Jaromir Lukavský, Petr Kastanek, Siba Prasad AdhikaryAbstract:Two filamentous cyanobacteria of the genera Scytonema and Tolypothrix were reported to be effective for stabilizing soil in arid areas due to the production of significant amounts of extracellular polysaccharides (EPS). These EPS may also have applications in the biotechnology industry. Therefore, two cyanobacterial species, Scytonema tolypothrichoides and Tolypothrix bouteillei were examined using crossed gradients of temperature (8-40°C) and irradiance (3-21 W m-2) to identify their temperature and irradiance optima for maximum biomass and EPS production. According to their reported temperature requirements, both strains were considered mesophilic. The optimum growth range of temperature in S. tolypothrichoides (27 to 34°C) was higher than T. bouteillei (22-32°C). The optimum irradiance range for growth of S. tolypothrichoides (9-13 W m-2) was slightly lower than T. bouteillei (7-18 W m-2). Maximum EPS production by S. tolypothrichoides occurred at similar temperatures (28-34°C) as T. bouteillei (27-34°C), both slightly higher than for maximum growth. The optimum irradiance range for EPS production was comparable to that for growth in S. tolypotrichoides (8-13 W m-2), and slightly lower in T. bouteillei (7-17 W m-2). The Redundancy Analysis confirmed that temperature was the most important controlling factor and protocols for field applications or for mass cultivation can now be developed.
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deciphering the genome sequences of the hydrophobic cyanobacterium Scytonema tolypothrichoides vb 61278
Genome Announcements, 2015Co-Authors: Arijit Panda, Siba Prasad Adhikary, Deeksha Singh, Mathu Malar Chandrababunaidu, Gyan Prakash Mishra, Sushma Bhan, Sucheta TripathyAbstract:ABSTRACT Scytonema tolypothrichoides VB-61278, a terrestrial cyanobacterium, can be exploited to produce commercially important products. Here, we report for the first time a 10-Mb draft genome assembly of S. tolypothrichoides VB-61278, with 214 scaffolds and 7,148 putative protein-coding genes.
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draft genome sequence of the terrestrial cyanobacterium Scytonema millei vb511283 isolated from eastern india
Genome Announcements, 2015Co-Authors: Mathu Malar Chandrababunaidu, Siba Prasad Adhikary, Deeksha Singh, Gyan Prakash Mishra, Neha Sanghi, Arpita Ghorai, Madhavi Madduluri, Sucheta TripathyAbstract:ABSTRACT We report here the draft genome sequence of Scytonema millei VB511283, a cyanobacterium isolated from biofilms on the exterior of stone monuments in Santiniketan, eastern India. The draft genome is 11,627,246 bp long (11.63 Mb), with 118 scaffolds. About 9,011 protein-coding genes, 117 tRNAs, and 12 rRNAs are predicted from this assembly.
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Diversity of Micro-algae and Cyanobacteria on Building Facades and Monuments in India
Algae, 2008Co-Authors: Lakshmi Kumari Samad, Siba Prasad AdhikaryAbstract:Fifty seven taxa of Cyanobacteria and 15 taxa of Chlorophyta were recorded from the exterior of buildings and rock surfaces of monuments in different regions in India. Four cyanobacteria, e.g. Chroococcidiopsis kashayi, Pseudophormidium indicum, Plectonema puteale and Scytonema geitleri, and the green alga Trentepholia abietina var. tenue occur on the sub-aerial habitats throughout the year. In addition, five other green algae: Chlorococcum infusionum, Scenedesmus arcuatus, Trentepholia aurea, Gloeocystis polydermatica and Printzina effusa, and 18 other cyanobacteria taxa of the genera Chroococcus (5), Asterocapsa (1), Cyanosarcina (2), Gloeocapsa (7), Gloeothece (2) and Scytonema (1) occur on the sub-aerial surfaces enduring extreme temperature and desiccation during summer months of the tropics. During the rainy season, the warm and humid climatic regime coupled with availability of moisture supported an additional ten green algae and 29 cyanobacteria in eight and 17 genera, respectively. The green algal genera Klebsormidium, Stichococcus and Trebouxia, which are dominant in temperate regions, did not occur on the sub-aerial habitats in India, however, species of Gloeocapsa, Chroococcus, Chroococcidiopsis, Phormidium, Leptolyngbya, Nostoc, Scytonema, Chlorella and Trentepholia showed global occurrence in similar habitats.
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Reaction of blue-green algae of rice-field soils to pesticide application
Tropical Agriculture, 1992Co-Authors: Jayanti Sahu, Siba Prasad AdhikaryAbstract:The effect of four commonly used pesticides, Furadan (carbofuran, 3%), Sevin (carbaryl, 50%), Rogor (dimethoate, 30% EC) and Endotaf (endosulfan, 35% EC) on the blue-green algae of rice field soils was examined. The organochlorine, Endotaf, was found to be more toxic than the organophosphate, Rogor, and the carbamate pesticides, Sevin and Furadan, in that sequence. Lyngbya major, Gloeocapsa atrata, Calothrix parietina and Scytonema pascheri, all of which have well-defined sheaths, occurred in the soil in the presence of 1000, 500 and 250 ppm of the carbamate, organophosphate and organochlorine pesticides, respectively, where other blue-green algal forms couls not survive
