The Experts below are selected from a list of 177 Experts worldwide ranked by ideXlab platform
Ljiljana Dimitrijevic - One of the best experts on this subject based on the ideXlab platform.
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adjuvant dependence of aps pathology related low affinity antibodies during Secondary Immune Response to tetanus toxoid in balb c mice
Immunologic Research, 2013Co-Authors: Irena živkovic, Vladimir Petrusic, Rajna Dimitrijevic, Marijana Stojanovic, Ljiljana DimitrijevicAbstract:One of the established animal models for autoImmune disease antiphospholipid syndrome (APS) is TTd hyperimmunization of mice. Tetanus toxoid (TTd) and plasma protein β2GPI share structural homology so that immunization with TTd induces appearance of cross-reactive antibodies. In this paper, we have investigated the presence and dynamic of fluctuation of specific (anti-TTd) and auto (anti-β2GPI) antibodies induced in BALB/c mice during Secondary Immune Response after TTd immunization with alhydrogel or glycerol as adjuvants. In addition, we followed the induced reproductive pathology as a sign of autoImmune outcome. We show undoubtedly adjuvant dependance of (1) level of induced anti-TTd IgG antibodies, (2) changes in levels of low-affinity anti-β2GPI IgG antibodies, and (3) change in fecundity and fertility during Secondary Immune Response. These findings once more indicate the importance of chosen adjuvants used for successful immunization and eventual autoantibody outcome, this time associated with the processes involving low affinity, natural antibodies.
Ljiljana Dimitrijević - One of the best experts on this subject based on the ideXlab platform.
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Adjuvant dependence of APS pathology-related low-affinity antibodies during Secondary Immune Response to tetanus toxoid in BALB/c mice
Immunologic Research, 2013Co-Authors: Irena Živković, Vladimir Petrušić, Rajna Dimitrijević, Marijana Stojanović, Ljiljana DimitrijevićAbstract:One of the established animal models for autoImmune disease antiphospholipid syndrome (APS) is TTd hyperimmunization of mice. Tetanus toxoid (TTd) and plasma protein β_2GPI share structural homology so that immunization with TTd induces appearance of cross-reactive antibodies. In this paper, we have investigated the presence and dynamic of fluctuation of specific (anti-TTd) and auto (anti-β_2GPI) antibodies induced in BALB/c mice during Secondary Immune Response after TTd immunization with alhydrogel or glycerol as adjuvants. In addition, we followed the induced reproductive pathology as a sign of autoImmune outcome. We show undoubtedly adjuvant dependance of (1) level of induced anti-TTd IgG antibodies, (2) changes in levels of low-affinity anti-β_2GPI IgG antibodies, and (3) change in fecundity and fertility during Secondary Immune Response. These findings once more indicate the importance of chosen adjuvants used for successful immunization and eventual autoantibody outcome, this time associated with the processes involving low affinity, natural antibodies.
N Amerio - One of the best experts on this subject based on the ideXlab platform.
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oxygen tension associated changes on Secondary Immune Response in halothane or isoflurane anesthetized mice
Acta Anaesthesiologica Scandinavica, 1995Co-Authors: N R Puig, G Elena, J Barragan, J O Comba, N AmerioAbstract:The impact that reexposure to anesthetics delivered in 100% oxygen or in synthetic air (21% oxygen/79% nitrogen) has on the Secondary humoral Immune Response to sheep red blood cells was studied. Mice were immunized twice with a 15-day interval and anesthetized immediately after each antigenic challenge with 1.5% halothane or 1.5% isoflurane for 40 min. Halothane in oxygen resulted in increased numbers of IgG-secreting cells (IgG-SC), while halothane in air depressed the Response when compared to control mice. In contrast, isoflurane vaporized in oxygen did not affect IgG-SC numbers, while isoflurane given in air lowered the Response. Furthermore, neither 100% oxygen, nor the stress of being in an anesthesia chamber breathing synthetic air for 40 min had any immunological effect in non-anesthetized mice. The inspired oxygen concentration during halothane or isoflurane anesthesia has an effect on the Secondary Immune Response. The effect is different between halothane and isoflurane, possibly due to differences in the extent of their metabolic and pharmacodynamic properties.
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halothane associated enhancement of the Secondary Immune Response to sheep erythrocytes in mice cell transfer studies
Acta Anaesthesiologica Scandinavica, 1993Co-Authors: N R Puig, G Elena, J Barragan, J O Comba, N AmerioAbstract:: The effect of halothane anesthesia on the humoral Immune Response to sheep red blood cells was studied in mice immunized twice, with a 15-day interval. On both occasions, mice were exposed to 1.5% halothane for 40 min immediately after sensitization. Halothane reexposure resulted in increased numbers of IgG-secreting cells (IgG-SC) as well as circulating 7S-serum agglutinins. To examine further whether this effect could be obtained in syngeneic recipients, adoptive transfer experiments employing spleen cells were performed. While mice receiving cells from unimmunized and anesthetized donors displayed significantly higher levels of IgG-SC, recipients of cells from normal, immunized and immunized-anesthetized donors showed a depressed Response when compared to control counterparts. Besides the possibility of an enhancing effect of halothane reexposure on the humoral Response, this procedure may counteract normal physiological immunoregulatory processes during the generation of the Immune Response.
Kazuo Sugane - One of the best experts on this subject based on the ideXlab platform.
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functional analysis of human memory b cell subpopulations igd cd27 b cells are crucial in Secondary Immune Response by producing high affinity igm
Clinical Immunology, 2003Co-Authors: Kazunaga Agematsu, Hans D Ochs, Kazuo SuganeAbstract:Abstract The number of memory B cells in peripheral blood has been assayed in various diseases by using CD27 as a memory B-cell marker. However, the defining differences of characteristic and function between the two memory B-cell subpopulations separated by immunoglobulin (Ig)D expression remain to be clearly elucidated. We analyzed here IgD+CD27+ B cells (circulating B cells 2, cB2) and IgD−CD27+ memory B cells (cB3) in comparison with IgD+CD27− naive B cells (cB1). cB2 were found to be morphologically similar to cB3 with abundant cytoplasm, whereas cB3 expressed CD80, CD86, and CD95 on their surface more predominantly than cB2. A majority of cB2 expressed both IgD and IgM, and cB3 expressed IgA or IgG. Mature γ1 and γ2 transcripts were found in cB3, but at very low levels in cB2, and activation-induced cytidine deaminase (AID) mRNA expression was recognized only in cB3. The frequencies of somatic hypermutation in cB2 and cB3 were comparable levels studied by VH5. cB2 did not shift to cB3 in vitro by the stimuli such as via B-cell receptor or CD40. cB2 produced large amounts of IgM predominantly and promptly, which is in accordance with the known characteristics of memory B cells. Taken together, although cB2 are unclass-switched, cB2 have the functions of memory B cells and are not in the process of transition from naive to switched memory B cells, playing a crucial role in Secondary Immune Response by producing high-affinity IgM in the early phase of infections.
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Functional analysis of human memory B-cell subpopulations: IgD+CD27+ B cells are crucial in Secondary Immune Response by producing high affinity IgM.
Clinical Immunology, 2003Co-Authors: Kazunaga Agematsu, Hans D Ochs, Kazuo SuganeAbstract:Abstract The number of memory B cells in peripheral blood has been assayed in various diseases by using CD27 as a memory B-cell marker. However, the defining differences of characteristic and function between the two memory B-cell subpopulations separated by immunoglobulin (Ig)D expression remain to be clearly elucidated. We analyzed here IgD+CD27+ B cells (circulating B cells 2, cB2) and IgD−CD27+ memory B cells (cB3) in comparison with IgD+CD27− naive B cells (cB1). cB2 were found to be morphologically similar to cB3 with abundant cytoplasm, whereas cB3 expressed CD80, CD86, and CD95 on their surface more predominantly than cB2. A majority of cB2 expressed both IgD and IgM, and cB3 expressed IgA or IgG. Mature γ1 and γ2 transcripts were found in cB3, but at very low levels in cB2, and activation-induced cytidine deaminase (AID) mRNA expression was recognized only in cB3. The frequencies of somatic hypermutation in cB2 and cB3 were comparable levels studied by VH5. cB2 did not shift to cB3 in vitro by the stimuli such as via B-cell receptor or CD40. cB2 produced large amounts of IgM predominantly and promptly, which is in accordance with the known characteristics of memory B cells. Taken together, although cB2 are unclass-switched, cB2 have the functions of memory B cells and are not in the process of transition from naive to switched memory B cells, playing a crucial role in Secondary Immune Response by producing high-affinity IgM in the early phase of infections.
Irena Živković - One of the best experts on this subject based on the ideXlab platform.
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Adjuvant dependence of APS pathology-related low-affinity antibodies during Secondary Immune Response to tetanus toxoid in BALB/c mice
Immunologic Research, 2013Co-Authors: Irena Živković, Vladimir Petrušić, Rajna Dimitrijević, Marijana Stojanović, Ljiljana DimitrijevićAbstract:One of the established animal models for autoImmune disease antiphospholipid syndrome (APS) is TTd hyperimmunization of mice. Tetanus toxoid (TTd) and plasma protein β_2GPI share structural homology so that immunization with TTd induces appearance of cross-reactive antibodies. In this paper, we have investigated the presence and dynamic of fluctuation of specific (anti-TTd) and auto (anti-β_2GPI) antibodies induced in BALB/c mice during Secondary Immune Response after TTd immunization with alhydrogel or glycerol as adjuvants. In addition, we followed the induced reproductive pathology as a sign of autoImmune outcome. We show undoubtedly adjuvant dependance of (1) level of induced anti-TTd IgG antibodies, (2) changes in levels of low-affinity anti-β_2GPI IgG antibodies, and (3) change in fecundity and fertility during Secondary Immune Response. These findings once more indicate the importance of chosen adjuvants used for successful immunization and eventual autoantibody outcome, this time associated with the processes involving low affinity, natural antibodies.
