The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform
John W Kappler - One of the best experts on this subject based on the ideXlab platform.
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an inverse relationship between t cell receptor affinity and antigen dose during cd4 t cell responses in vivo and in vitro
Proceedings of the National Academy of Sciences of the United States of America, 1999Co-Authors: William Rees, Jeremy Bender, Kent T Teague, Frances Crawford, Ross M Kedl, Philippa Marrack, John W KapplerAbstract:Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on Secondary Immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent receptor affinity of the T cells that were present several months after a primary response or after a Secondary stimulation either in vivo or in vitro.
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An inverse relationship between T cell receptor affinity and antigen dose during CD4(+) T cell responses in vivo and in vitro.
Proceedings of the National Academy of Sciences of the United States of America, 1999Co-Authors: William Rees, Jeremy Bender, Frances Crawford, T. Kent Teague, Ross M Kedl, Philippa Marrack, John W KapplerAbstract:Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on Secondary Immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent receptor affinity of the T cells that were present several months after a primary response or after a Secondary stimulation either in vivo or in vitro.
D. M. Vasudevan - One of the best experts on this subject based on the ideXlab platform.
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Secondary Immunization inhibits drug induced apoptosisin vivo.
Indian journal of clinical biochemistry : IJCB, 2003Co-Authors: Winston Costa Pereira, Muddanna S. Rao, D. M. VasudevanAbstract:Immunization with a proper dose of an antigenic stimulus leads to cell proliferation and antibody response of circulating lymphocytes. We have previously observed that Secondary immunized spleenocytes resist ceramide-mediated apoptosisin vitro. Our present study is aimed at investigating thein vivo effect of Immunization on apoptosis. Mice were subjected to either Primary or Secondary dose with Tetanus Toxoid. Unimmunized spleenocytes served as controls. Unimmunized, Primary and Secondary immunized mice were later exposed to chemotherapeutic drugs such as Etoposide/Methotrexate/Vincristine to induce apoptosis. Apoptosis was studied by using the Feulgen reaction on 5μ thin parafin sections of spleen. It was observed that Secondary immunized mice showed a lower percentage of apoptosis as compared to Primary or Unimmunized mice that was subjected to either of the chemotherapeutic drugs. It was thus concluded that Secondary Immunization inhibits the process of chemotherapeutic drug induced apoptosis in vivo.
George R. Siber - One of the best experts on this subject based on the ideXlab platform.
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Pertussis toxin enhanced IgG1 and IgE responses to primary tetanus Immunization are mediated by interleukin-4 and persist during Secondary responses to tetanus alone
Vaccine, 1996Co-Authors: Matthew H. Samore, George R. SiberAbstract:Abstract Pertussis toxin (Ptx), the major toxin product of Bordetella pertussis, has potent immunologic effects including adjuvant effects on antibody responses and sensitization for anaphylaxis. In order to further define the effect of Ptx on the class and subclass of murine antibody response, we measured total and antigen specific IgG subclasses and IgE in Balb c mice after primary and Secondary Immunization with tetanus toxoid (TT). Low doses of Ptx (100 ng) given intravenously at the time of primary Immunization increased primary IgG1 and IgE anti-TT antibodies as well as total IgG1 and IgE concentrations compared to controls. The increase in IgG1 subclass and IgE response when Ptx was present during primary Immunization was even more pronounced after Secondary Immunization with TT alone 3 weeks or 3 months later. Similar effects were noted after diphtheria toxoid Immunization in the presence of Ptx. Administration of the anti IL-4 monoclonal antibody (11B11) suppressed the enhanced total and TT-specific IgE responses but not the enhanced IgG1 responses. The presence of low concentrations of Ptx during primary Immunization primes for induction of IL-4 producing T-cell help which enhances IgG1 and IgE responses to the primary exposure as well as to subsequent exposures of the antigen in the absence of Ptx. This phenomenon may have significance for the adjuvant activity of vaccines containing Ptx as well as for the immune response to natural pertussis.
Mark M Davis - One of the best experts on this subject based on the ideXlab platform.
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a kinetic basis for t cell receptor repertoire selection during an immune response
Immunity, 1999Co-Authors: Peter A Savage, Jay J Boniface, Mark M DavisAbstract:The basis for T cell antigen receptor (TCR) repertoire selection upon repeated antigenic challenge is unclear. We evaluated the avidity and dissociation kinetics of peptide/major histocompatibility complex (MHC) tetramer binding to antigen-specific T lymphocytes isolated following primary or Secondary Immunization. The data reveal a narrowing of the Secondary repertoire relative to the primary repertoire, largely resulting from the loss of cells expressing TCRs with the fastest dissociation rates for peptide/MHC binding. In addition, T cells in the Secondary response express TCRs of higher average affinity for peptide/MHC than cells in the primary response. These results provide a link between the kinetics and affinity of TCR-peptide/MHC interactions and TCR sequence selection during the course of an immune response.
Thomas Dörner - One of the best experts on this subject based on the ideXlab platform.
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Correction: Secondary Immunization Generates Clonally Related Antigen-Specific Plasma Cells and Memory B Cells
The Journal of Immunology, 2013Co-Authors: Daniela Frölich, Claudia Giesecke, Henrik E. Mei, Karin Reiter, Capucine Daridon, Peter E. Lipsky, Thomas DörnerAbstract:Frolich, D., C. Giesecke, H. E. Mei, K. Reiter, C. Daridon, P. E. Lipsky, and T. Dorner. 2010. Secondary Immunization generates clonally related antigen-specific plasma cells and memory B cells. J. Immunol. 185: [3103–3110][1]. In [Fig. 1][2], the dot plot shown at day 5 was incorrectly shown
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Secondary Immunization generates clonally related antigen specific plasma cells and memory b cells
Journal of Immunology, 2010Co-Authors: Daniela Frölich, Claudia Giesecke, Henrik E. Mei, Karin Reiter, Capucine Daridon, Peter E. Lipsky, Thomas DörnerAbstract:Rechallenge with T cell-dependent Ags induces memory B cells to re-enter germinal centers (GCs) and undergo further expansion and differentiation into plasma cells (PCs) and Secondary memory B cells. It is currently not known whether the expanded population of memory B cells and PCs generated in Secondary GCs are clonally related, nor has the extent of proliferation and somatic hypermutation of their precursors been delineated. In this study, after Secondary tetanus toxoid (TT) Immunization, TT-specific PCs increased 17- to 80-fold on days 6-7, whereas TT-specific memory B cells peaked (delayed) on day 14 with a 2- to 22-fold increase. Molecular analyses of V(H)DJ(H) rearrangements of individual cells revealed no major differences of gene usage and CDR3 length between TT-specific PCs and memory B cells, and both contained extensive evidence of somatic hypermutation with a pattern consistent with GC reactions. This analysis identified clonally related TT-specific memory B cells and PCs. Within clusters of clonally related cells, sequences shared a number of mutations but also could contain additional base pair changes. The data indicate that although following Secondary Immunization PCs can derive from memory B cells without further somatic hypermutation, in some circumstances, likely within GC reactions, asymmetric mutation can occur. These results suggest that after the fate decision to differentiate into Secondary memory B cells or PCs, some committed precursors continue to proliferate and mutate their V(H) genes.