Semitendinous Muscle

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Alexandre Donizete Ferreira - One of the best experts on this subject based on the ideXlab platform.

  • Analise fenotipica do musculo semitendinoso de ratos wistar e os efeitos do treinamento intervalado sobre suas fibras musculares
    Universidade Estadual de Campinas. Instituto de Biologia, 2004
    Co-Authors: Alexandre Donizete Ferreira
    Abstract:

    Os músculos esqueléticos em geral são constituídos por vários tipos de fibras que expressam diferentes isoformas de miosina (MHC). As miosinas dos tipos I, IIa, Iid/x e IIb estão presentes nas fibras puras dos tipos I, IIA, IID/X e IIB, respectivamente. Entre as fibras puras existem as fibras híbridas, que podem expressar duas ou mais isoformas de miosina. Nesse trabalho foi verificado, através da técnica histoquímica de mATPase e da técnica de separação eletroforética de proteínas, os tipos de fibras que compõe o músculo semitendinoso de ratos Wistar e as respectivas isoformas de miosina. Também foi avaliado como o treinamento intervalado pode influenciar na plasticidade dessas fibras. Os resultados mostraram que o músculo semitendinoso possui duas porções distintas, uma vermelha e outra branca. Apresenta todos os tipos de fibras puras, com predominância das fibras do tipo IIB, caracterizando-o como um músculo de contração rápida. Observou-se também a presença de fibras híbridas dos tipos IC, IIC, IIAD e IIDB. O treinamento promoveu alterações nas fibras musculares, em especial nas fibras do grupo II. Em conclusão, o fenótipo do músculo semitendinoso é de contração rápida e o treinamento intervalado foi capaz de promover aumento do número de fibras híbridas IC, IIAD e IIDB, bem como, aumento da secção transversal das fibras IIDB e IIBIn general, the skeletal Muscles are formed to several Muscle fiber types that are able to express different kinds of myosin heavy chain (MHC) isoforms. The MHCI, IIa, IId and IIb will establish the pure Muscle fibers of the type I, IIA, IID and IIB, respectively and, among them there are the hybrid fibers that can express two or more MHC types. In this work was verified through mATPase histochemical and eletophoretic separation techniques which myosin isoforms and hybrids make the Semitendinous Muscle of the Wistar rats and also how intermittent training influence in the plasticity of these fibers. It was seen that this Muscle has two distinct portions, one red portion and one white portion and that was meeting all pure fibers, where the IIB type was seen in large number, characterized the Semitendinous how Muscle of fast twitch. There was also observed the presence of the IC, IIC, IIAD, IIDB hybrid fibers. The training promoted changes in the Muscle fibers, in special in the hybrid fibers of the group II and promoted increase in the cross-sectional area of some glycolytic fibers (IIDB and IIB). In conclusion, the phenotype of the Semitendinous Muscle is formed in great part by fast-twitch fibers and the training was able to promote changes in the MHC isoforms present in these Muscle fiber

Alexandre Donizete - One of the best experts on this subject based on the ideXlab platform.

  • Analise fenotipica do musculo semitendinoso de ratos wistar e os efeitos do treinamento intervalado sobre suas fibras musculares
    [s.n.], 2018
    Co-Authors: Alexandre Donizete
    Abstract:

    Orientador: Gerson Eduardo RochaDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de BiologiaResumo: Os músculos esqueléticos em geral são constituídos por vários tipos de fibras que expressam diferentes isoformas de miosina (MHC). As miosinas dos tipos I, IIa, Iid/x e IIb estão presentes nas fibras puras dos tipos I, IIA, IID/X e IIB, respectivamente. Entre as fibras puras existem as fibras híbridas, que podem expressar duas ou mais isoformas de miosina. Nesse trabalho foi verificado, através da técnica histoquímica de mATPase e da técnica de separação eletroforética de proteínas, os tipos de fibras que compõe o músculo semitendinoso de ratos Wistar e as respectivas isoformas de miosina. Também foi avaliado como o treinamento intervalado pode influenciar na plasticidade dessas fibras. Os resultados mostraram que o músculo semitendinoso possui duas porções distintas, uma vermelha e outra branca. Apresenta todos os tipos de fibras puras, com predominância das fibras do tipo IIB, caracterizando-o como um músculo de contração rápida. Observou-se também a presença de fibras híbridas dos tipos IC, IIC, IIAD e IIDB. O treinamento promoveu alterações nas fibras musculares, em especial nas fibras do grupo II. Em conclusão, o fenótipo do músculo semitendinoso é de contração rápida e o treinamento intervalado foi capaz de promover aumento do número de fibras híbridas IC, IIAD e IIDB, bem como, aumento da secção transversal das fibras IIDB e IIBAbstract: In general, the skeletal Muscles are formed to several Muscle fiber types that are able to express different kinds of myosin heavy chain (MHC) isoforms. The MHCI, IIa, IId and IIb will establish the pure Muscle fibers of the type I, IIA, IID and IIB, respectively and, among them there are the hybrid fibers that can express two or more MHC types. In this work was verified through mATPase histochemical and eletophoretic separation techniques which myosin isoforms and hybrids make the Semitendinous Muscle of the Wistar rats and also how intermittent training influence in the plasticity of these fibers. It was seen that this Muscle has two distinct portions, one red portion and one white portion and that was meeting all pure fibers, where the IIB type was seen in large number, characterized the Semitendinous how Muscle of fast twitch. There was also observed the presence of the IC, IIC, IIAD, IIDB hybrid fibers. The training promoted changes in the Muscle fibers, in special in the hybrid fibers of the group II and promoted increase in the cross-sectional area of some glycolytic fibers (IIDB and IIB). In conclusion, the phenotype of the Semitendinous Muscle is formed in great part by fast-twitch fibers and the training was able to promote changes in the MHC isoforms present in these Muscle fibersMestradoAnatomiaMestre em Biologia Celular e Estrutura

Jorge H. Calvo - One of the best experts on this subject based on the ideXlab platform.

  • The effect of feeding system in the expression of genes related with fat metabolism in Semitendinous Muscle in sheep.
    Meat Science, 2011
    Co-Authors: Enkeleda Dervishi, Clementina Rodellar, Carmen Serrano, Martina Pérez Serrano, Jorge H. Calvo
    Abstract:

    Abstract The effect of feeding system on the expression of LPL, ACACA, FASN, FABP4, DGAT1, SCD, CPT1B, PRKAA2, LEP, SREBP1, PPARG, PPARA and CEBPB genes in Semitendinous Muscle was studied. Forty-four single born male lambs of the Rasa Aragonesa breed, allocated to four different dietary treatments, were used: grazing alfalfa, grazing alfalfa with supplement for lambs, indoor lambs with grazing ewes and drylot. Significant differences were found in the expression of genes LPL, ACACA, FASN, FABP4, CPT1B and SCD. Genes related to adipogenesis (LPL, ACACA, FASN, FABP4, and SCD) are up-regulated in the intensive groups. In grazing groups CPT1B gene expression, related to β-oxidation process, is up-regulated. The relative expression of CPT1B was 1.54 fold higher in ALF + S, and 0.43 and 0.37 fold lower in IND- GRE and IND, respectively. The results support the hypothesis that changes in fatty acid profile due to feeding system implicate changes in the mRNA expression level of genes related with fat metabolism. Feeding strategy is an important tool to manipulate intramuscular fatty acid profile in meat through altering gene expression of enzymes related with fat metabolism.

  • Effect of the feeding system on the fatty acid composition, expression of the Δ^9-desaturase, Peroxisome Proliferator-Activated Receptor Alpha, Gamma, and Sterol Regulatory Element Binding Protein 1 genes in the Semitendinous Muscle of light lambs of
    BMC Veterinary Research, 2010
    Co-Authors: Enkeleda Dervishi, Clementina Rodellar, Carmen Serrano, Margalida Joy, Malena Serrano, Jorge H. Calvo
    Abstract:

    Background Conjugated linoleic acids (CLAs) are receiving increasing attention because of their beneficial effects on human health, with milk and meat products derived from ruminants as important sources of CLA in the human diet. SCD gene is responsible for some of the variation in CLA concentration in adipose tissues, and PPARγ , PPARα and SREBP1 genes are regulator of SCD gene. The aim of this work was to evaluate the effect of the feeding system on fatty acid composition, CLA content and relative gene expression of Δ^9-desaturase ( SCD ), Peroxisome Proliferator-Activated Receptor Gamma ( PPARγ ), Peroxisome Proliferator-Activated Receptor Alpha, (PPARα) and Sterol Regulatory Element Binding Protein (SREBP1) in Rasa Aragonesa light lambs in Semitendinous Muscle. Forty-four single-born male lambs were used to evaluate the effect of the feeding system, varying on an intensity gradient according to the use of concentrates: 1. grazing alfalfa, 2. grazing alfalfa with a supplement for lambs, 3. indoor lambs with grazing ewes and 4. drylot. Results Both grazing systems resulted in a higher concentration of vaccenic acid (VA), CLA, CLA/VA acid ratio, and a lower oleic content, oleic acid (C18:1)/stearic acid (C18:0) ratio, PUFA n-6/n-3 ratio and SCD expression compared to other diets. In addition feeding system affected the fatty acid composition and SCD expression, possibly due to CLA concentration or the PUFA n-6/n-3 ratio. Both expression of the SCD gene and the feeding system were important factors affecting CLA concentration in the animal's Semitendinous Muscle. PPARγ, PPARα and SREBP1 expression seemed to be unaffected by the feeding system. Although no significant results were found, PPARγ, PPARα and SREBP1 showed similar expression pattern as SCD . Moreover, the correlation results between SCD expression and PPARγ (p < 0.01), as well as SREBP1 (p < 0.01) expression, may suggest that these genes were affecting SCD expression in a different way. Conclusions The data indicated that the feeding system is the main factor affecting the fatty acid composition and SCD gene expression, which is also affected by CLA and possibly by n-6/n-3 PUFAs.

Arlete Mazzini Miranda Giovani - One of the best experts on this subject based on the ideXlab platform.

  • CLINICS 2010;65(3):297-303 BASIC RESEARCH HISTOLOGICAL STUDY OF FRESH VERSUS FROZEN Semitendinous Muscle TENDON ALLOGRAFTS
    2013
    Co-Authors: Re Carneiro Bitar, Edgard Novaes França Bisneto, Luiz Augusto, Ubirajara Santos, Alberto Tesconi, João Alberto, Ramos Maradei Pereira, Mazzini Mir, Arlete Mazzini Miranda Giovani
    Abstract:

    of fresh versus frozen Semitendinous Muscle tendon allografts. Clinics. 2010;65(3):297-303. OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric Semitendinous Muscle after cryopreservation at-80°C in comparison to a control group kept at only-4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 Semitendinous tendons from 10 cadavers were frozen at-80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still “fresh”. RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous Muscle tendon allografts can be submitted to cryopreservation at-80ºC without suffering histological modifications

  • Histological study of fresh versus frozen Semitendinous Muscle tendon allografts
    Clinics (Sao Paulo Brazil), 2010
    Co-Authors: Alexandre Carneiro Bitar, Luiz Augusto Ubirajara Santos, Alberto Tesconi Croci, João Alberto Ramos Maradei Pereira, Edgard Novaes França Bisneto, Arlete Mazzini Miranda Giovani, Claudia Regina G. C. M. Oliveira
    Abstract:

    OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric Semitendinous Muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 Semitendinous tendons from 10 cadavers were frozen at -80ÂoC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous Muscle tendon allografts can be submitted to cryopreservation at -80ÂoC without suffering histological modifications.

Campos G.e.r. - One of the best experts on this subject based on the ideXlab platform.

  • Fiber Type Composition In Semitendinous Muscle Of Wistar Rats And Effects Of Intermittent Training On Its Hypertrophy
    2015
    Co-Authors: Ferreira A., Campos G.e.r.
    Abstract:

    Skeletal Muscles respond to several stimuli changing their phenotype. Muscular fibers adaptation capability is related to the presence of several myosin heavy chains (MHC). These express four types of pure fibers: I, IIA, IID and IIB containing MHCI, IIa, IId and IIb, respectively. Among pure fibers, there are hybrid fibers, which can express two or more types of myosins. In this study, types of fibers constituting male Wistar rats Semitendinous and their myosin heavy chains, as well as influence of intermittent training on hypertrophy of these fibers have been checked through MATPase histochemical technique and electrophoretic proteins separation. All types of pure and hybrid muscular fiber have been found, however the fibers of the types IIA, IID and IIB were predominant, featuring Muscle as a fast-contracting one. Training has promoted muscular fibers transition with a significant increase of fibers of IC, IIAD and IIDB type. A cross-section increase of fibers of IIDB and IIB type has also been noticed. In summary, Semitendinous Muscle is essentially constituted by fast-contracting fibers and training could promote transition and hypertrophy of these fast fibers

  • Fiber Type Composition In Semitendinous Muscle Of Wistar Rats And Effects Of Intermittent Training On Its Hypertrophy
    2015
    Co-Authors: Ferreira A., Campos G.e.r.
    Abstract:

    Skeletal Muscles respond to several stimuli changing their phenotype. Muscular fibers adaptation capability is related to the presence of several myosin heavy chains (MHC). These express four types of pure fibers: I, IIA, IID and IIB containing MHCI, IIa, IId and IIb, respectively. Among pure fibers, there are hybrid fibers, which can express two or more types of myosins. In this study, types of fibers constituting male Wistar rats Semitendinous and their myosin heavy chains, as well as influence of intermittent training on hypertrophy of these fibers have been checked through MATPase histochemical technique and electrophoretic proteins separation. All types of pure and hybrid muscular fiber have been found, however the fibers of the types IIA, IID and IIB were predominant, featuring Muscle as a fast-contracting one. Training has promoted muscular fibers transition with a significant increase of fibers of IC, IIAD and IIDB type. A cross-section increase of fibers of IIDB and IIB type has also been noticed. In summary, Semitendinous Muscle is essentially constituted by fast-contracting fibers and training could promote transition and hypertrophy of these fast fibers.2616367Andersen, J.L., Schjerling, P., Saltin, B., Muscle, genes, and athletic performance (2000) Sci. Am, 283, pp. 48-55Blum, H., Beier, H., Gross, H.J., Improved silver staining of plant-proteins, RNA and DNA in polyacrylamide gels (1987) Electrophoresis, 8 (2), pp. 93-99Bottinelli, R., Reggianni, C., Human skeletal Muscle fibres: Molecular and functional diversity (2000) Prog. Biophys. Mol. Bio, 73, pp. 195-262Brooke, M.H., Kaiser, K.K., Three "myosin adenosine triphosphatase" systems: The nature of their pH lability and sulfhydryl dependence (1970) J. Histochem. Cytochem, 18, pp. 670-672Caiozzo, V.J., Baker, M.J., Huang, K., Chou, H., Wu, Y.Z., Baldwin, K.M., Single-fiber myosin heavy chain polymorphism: How many patterns and what proportions? (2003) Am. J. Physiol. Regul. Integr. Comp. Physiol, 285, pp. R570-R580Campos, G.E.R., Luecke, T., Wendeln, H.K., Toma, K., Hagerman, F.C., Murray, T.F., Ragg, K.E., Staron, R.S., Muscular adaptationsin response to three different resistance training regimens: Specificity of repetition maximum training zones (2002) Eur. J. Appl. Physiol, 88, pp. 50-60Delp, M.D., Duan, C., Composition and size of type I, IIA, IID/X and IIB fibers and citrate synthase activity of rat Muscle (1996) J. Appl. Physiol, 80, pp. 261-270Demirel, H.A., Powers, S.K., Naito, H., Hughes, M., Coombes, J.S., Exercise-induced alterations in skeletal Muscle myosin heavy chain phenotype: Dose-response relationship (1999) J. Appl. Physiol, 86 (3), pp. 1002-1008Goldispink, G., Gene expression in Muscle in response to exercise (2003) J. Muscle Res. Cell, 24, pp. 121-126Guth, L., Samaha, F.J., Procedure for the histochemical demonstration of actomyosin ATPase (1970) Exp. Neurol, 28, pp. 365-367Harrison, B.C., Bell, M.L., Allen, D.L., Byrnes, W.C., Leinwand, L.A., Skeletal Muscle adaptations in response to voluntary wheel running in myosin heavy chain null mice (2002) J. Appl. Physiol, 92, pp. 313-322Jurie, C., Picard, B., Geay, Y., Influences of the method of housing bulls on their body composition and Muscle fibre types (1998) Meat Science, 50, pp. 457-469Lin, J., Wu, H., Tarr, P.T., Zhang, C.Y., Wu, Z., Boss, O., Michael, L.F., Spiegelman, B., Transcriptional co-activator PGC-1a drives the formation of slow-twich Muscle fibres (2002) Nature, 418, pp. 797-801Pette, D., Staron, R.S., Transitions of Muscle fiber phenotypic profiles (2001) Histochem. Cell Biol, 115, pp. 359-372Smolka, M.B., Zoppi, C.C., Alvez, A.A., Silveira, L.R., Marangoni, S., Silva, L.P., Novello, J., Macedo, D.V., HSP72 as a complymentary protection against oxidative stress induced by exercise in the soleus Muscle of rats (2000) Am. J. Physiol. Regulatory Integrative Comp. Physiol, 279, pp. R1539-R1545Staron, R.S., Kraemer, W.J., Hikida, R.S., Fry, A.C., Murray, J.D., Campos, G.E.R., Fiber type composition of four hindlimb Muscles of adult Fisher 344 rats (1999) Histochem. Cell Biol, 111, pp. 117-123Tajsharghi, H., Sunnerhagen, K.S., Darin, N., Kyllerman, M., Oldfors, A., Induced shift in myosin heavy chain expression in myosin myopathy by endurance training (2004) J. Neurol, 251, pp. 179-183Talmadge, R.J., Myosin heavy chain isoform expression following reduced neuromuscular activity: Potential regulatory mechanisms (2000) Muscle Nerve, 23, pp. 661-67

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