The Experts below are selected from a list of 7140 Experts worldwide ranked by ideXlab platform
Francis Barin - One of the best experts on this subject based on the ideXlab platform.
-
Comparative Analysis of Humoral Immune Responses to HIV Type 1 Envelope Glycoproteins in Mice Immunized with a DNA Vaccine, Recombinant Semliki Forest Virus RNA, or Recombinant Semliki Forest Virus Particles
AIDS research and human retroviruses, 1998Co-Authors: Denys Brand, Franck Lemiale, Isabelle Turbica, Laurence Buzelay, Sylvie Brunet, Francis BarinAbstract:ABSTRACT The Semliki Forest Virus (SFV) system seems to be a useful new approach for generating effective immune responses against HIV-1 in animal models. We evaluated this system by comparing the ...
-
comparative analysis of humoral immune responses to hiv type 1 envelope glycoproteins in mice immunized with a dna vaccine recombinant Semliki Forest Virus rna or recombinant Semliki Forest Virus particles
AIDS Research and Human Retroviruses, 1998Co-Authors: Denys Brand, Franck Lemiale, Isabelle Turbica, Laurence Buzelay, Sylvie Brunet, Francis BarinAbstract:ABSTRACT The Semliki Forest Virus (SFV) system seems to be a useful new approach for generating effective immune responses against HIV-1 in animal models. We evaluated this system by comparing the humoral immune responses raised in mice immunized against the HIV-1 envelope with the SFV system, a DNA vaccine, and a recombinant Env glycoprotein. gp160 ELISA antibody titers (204,800) were highest in the sera from mice immunized with recombinant Semliki Forest Virus particles. These sera contained antibodies to the CD4-binding site and recognized linear epitopes on gp120 and gp41 that were also recognized by a pool of sera from HIV-1-infected individuals. This demonstrates that the HIV-1 envelope produced in vivo by the SFV system does not fold aberrantly. A low level of neutralizing antibodies against the HIV-1LAI strain was also detected in the serum of one mouse immunized with recombinant SFV particles, suggesting that booster injections should be given to achieve a more effective immune response. SFV reco...
Denys Brand - One of the best experts on this subject based on the ideXlab platform.
-
Comparative Analysis of Humoral Immune Responses to HIV Type 1 Envelope Glycoproteins in Mice Immunized with a DNA Vaccine, Recombinant Semliki Forest Virus RNA, or Recombinant Semliki Forest Virus Particles
AIDS research and human retroviruses, 1998Co-Authors: Denys Brand, Franck Lemiale, Isabelle Turbica, Laurence Buzelay, Sylvie Brunet, Francis BarinAbstract:ABSTRACT The Semliki Forest Virus (SFV) system seems to be a useful new approach for generating effective immune responses against HIV-1 in animal models. We evaluated this system by comparing the ...
-
comparative analysis of humoral immune responses to hiv type 1 envelope glycoproteins in mice immunized with a dna vaccine recombinant Semliki Forest Virus rna or recombinant Semliki Forest Virus particles
AIDS Research and Human Retroviruses, 1998Co-Authors: Denys Brand, Franck Lemiale, Isabelle Turbica, Laurence Buzelay, Sylvie Brunet, Francis BarinAbstract:ABSTRACT The Semliki Forest Virus (SFV) system seems to be a useful new approach for generating effective immune responses against HIV-1 in animal models. We evaluated this system by comparing the humoral immune responses raised in mice immunized against the HIV-1 envelope with the SFV system, a DNA vaccine, and a recombinant Env glycoprotein. gp160 ELISA antibody titers (204,800) were highest in the sera from mice immunized with recombinant Semliki Forest Virus particles. These sera contained antibodies to the CD4-binding site and recognized linear epitopes on gp120 and gp41 that were also recognized by a pool of sera from HIV-1-infected individuals. This demonstrates that the HIV-1 envelope produced in vivo by the SFV system does not fold aberrantly. A low level of neutralizing antibodies against the HIV-1LAI strain was also detected in the serum of one mouse immunized with recombinant SFV particles, suggesting that booster injections should be given to achieve a more effective immune response. SFV reco...
Leevi Kääriäinen - One of the best experts on this subject based on the ideXlab platform.
-
ASSOCIATION OF CAPSID PROTEIN WITH Semliki Forest Virus MESSENGER RNAs
Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology, 2009Co-Authors: Hans Söderlund, Leevi KääriäinenAbstract:Semliki Forest Virus specific polysomes from infected HeLa cells were isolated. Part of the newly formed, radioactively labelled protein remained associated with the messenger RNA after release of the nascent polypeptide chains with EDTA: The messenger RNA bound label consisted mostly of viral capsid protein. Direct isolation of ribonucleoproteins from the cytoplasm showed that the capsid protein binds to both 42 S and 26 S viral RNAs.
-
RNA helicase activity of Semliki Forest Virus replicase protein NSP2.
FEBS Letters, 1999Co-Authors: Marta Gómez De Cedrón, Neda Ehsani, Juan Antonio García, Marja L Mikkola, Leevi KääriäinenAbstract:Semliki Forest Virus replicase protein nsP2 shares sequence homology with several putative NTPases and RNA helicases. NsP2 has RNA-dependent NTPase activity. Here we expressed polyhistidine-tagged nsP2 in Escherichia coli, purified it by metal-affinity chromatography, and used it in RNA helicase assays. RNA helicase CI of plum pox potyVirus was used as a positive control. Unwinding of α-32P-labelled partially double-stranded RNA required nsP2, Mg2+ and NTPs. NsP2 with a mutation, K192N, in the NTP-binding sequence GVPGSGK192SA could not unwind dsRNA and had no NTPase activity. This is the first demonstration of RNA helicase activity within the large alphaVirus superfamily.
-
Synthesis of Semliki Forest Virus RNA polymerase components nsP1 through nsP4 in Saccharomyces cerevisiae by expression of cDNA encoding the nonstructural polyprotein.
Journal of virology, 1996Co-Authors: P Russo, Pirjo Laakkonen, Tero Ahola, Leevi KääriäinenAbstract:A Semliki Forest Virus nonstructural polyprotein, P1234, expressed in the yeast Saccharomyces cerevisiae in the absence of a replicative RNA template appeared to be properly cleaved into nsP1 to nsP4. All nsPs were membrane associated, and nsP2 was also transported to the nucleus. The membrane fraction containing nsPs showed guanine-7-methyltransferase and guanylyltransferase-like activities, typical for Semliki Forest Virus nsP1.
Peter Liljeström - One of the best experts on this subject based on the ideXlab platform.
-
recombinant Semliki Forest Virus vaccine vectors the route of injection determines the localization of vector rna and subsequent t cell response
Gene Therapy, 2001Co-Authors: P Colmenero, Peter Liljeström, Peter Berglund, Taku Kambayashi, P Biberfeld, Mikael JondalAbstract:Recombinant Semliki Forest Virus vaccine vectors: the route of injection determines the localization of vector RNA and subsequent T cell response
-
self replicating Semliki Forest Virus rna as recombinant vaccine
Vaccine, 1994Co-Authors: Xianzheng Zhou, Peter Berglund, Gary Rhodes, S E Parker, Mikael Jondal, Peter LiljeströmAbstract:Recombinant RNA based on the Semliki Forest Virus (SFV) replicon was used to express the nucleoprotein of influenza Virus in mice. Two strategies were employed to deliver the RNA. In the first, recombinant RNA was packaged into infectious suicide SFV particles which were used directly for immunization. The second approach involved injection of in vitro-synthesized RNA directly into the quadriceps muscle. Both approaches resulted in the generation of humoral responses with high antibody titres. Immunization with suicide particles showed that a strong, class I-restricted cytotoxic T-cell response can be obtained using only 100 infectious units. We conclude that the self-replicative recombinant SFV RNA may be quite useful as a nucleic acid vaccine.
Mikael Jondal - One of the best experts on this subject based on the ideXlab platform.
-
recombinant Semliki Forest Virus vaccine vectors the route of injection determines the localization of vector rna and subsequent t cell response
Gene Therapy, 2001Co-Authors: P Colmenero, Peter Liljeström, Peter Berglund, Taku Kambayashi, P Biberfeld, Mikael JondalAbstract:Recombinant Semliki Forest Virus vaccine vectors: the route of injection determines the localization of vector RNA and subsequent T cell response
-
self replicating Semliki Forest Virus rna as recombinant vaccine
Vaccine, 1994Co-Authors: Xianzheng Zhou, Peter Berglund, Gary Rhodes, S E Parker, Mikael Jondal, Peter LiljeströmAbstract:Recombinant RNA based on the Semliki Forest Virus (SFV) replicon was used to express the nucleoprotein of influenza Virus in mice. Two strategies were employed to deliver the RNA. In the first, recombinant RNA was packaged into infectious suicide SFV particles which were used directly for immunization. The second approach involved injection of in vitro-synthesized RNA directly into the quadriceps muscle. Both approaches resulted in the generation of humoral responses with high antibody titres. Immunization with suicide particles showed that a strong, class I-restricted cytotoxic T-cell response can be obtained using only 100 infectious units. We conclude that the self-replicative recombinant SFV RNA may be quite useful as a nucleic acid vaccine.
