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Brian J Steffenson - One of the best experts on this subject based on the ideXlab platform.
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association mapping of Septoria speckled leaf blotch resistance in u s barley breeding germplasm
Phytopathology, 2013Co-Authors: Hao Zhou, Brian J SteffensonAbstract:ABSTRACT Septoria speckled leaf blotch (SSLB) is a sporadic but important disease of barley (Hordeum vulgare) in the Upper Midwest region of the United States and Prairie Provinces of Canada that is caused primarily by Septoria passerinii. Most of the widely grown cultivars in the region are susceptible to the disease. To identify and map SSLB resistance loci in U.S. barley breeding germplasm, we employed an association mapping approach using 3,840 breeding lines and cultivars and nearly 3,000 single-nucleotide polymorphism markers previously mapped to the seven barley chromosomes. SSLB infection responses (IRs) were assayed on seedling plants in the greenhouse using a 0-to-5 scale. From the analysis of four yearly panels consisting of 960 lines each, four quantitative trait loci (QTL) for SSLB resistance were identified: one on chromosome 1H (Rsp-qtl-H_12_31144), one on chromosome 3H (Rsp-qtl-3H_12_31488), and two on chromosome 6H (Rsp-qtl-6H_11_21032 and Rsp-qtl-6H_11_10064). Individual resistance QTL r...
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molecular mapping and marker assisted selection of genes for Septoria speckled leaf blotch resistance in barley
Phytopathology, 2006Co-Authors: Shaobin Zhong, Hala Toubiarahme, Brian J Steffenson, Kevin P SmithAbstract:Zhong, S., Toubia-Rahme, H., Steffenson, B. J., and Smith, K. P. 2006. Molecular mapping and marker-assisted selection of genes for Septoria speckled leaf blotch resistance in barley. Phytopathology 96:993-999. Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii, has emerged as one of the most important foliar diseases of barley in the Upper Midwest region of the United States. To map and tag genes for SSLB resistance, we developed two populations derived from the resistant accessions CIho 4780 and CIho 10644 and the susceptible malting cv. Foster. Segregation analysis of F2 plants or F2:3 families from the Foster/CIho 4780 and Foster/CIho 10644 populations revealed that a single dominant gene conferred resistance at the seedling stage. Bulked segregant analysis identified an amplified fragment length polymorphism marker, E-ACT/M-CAA-170, that co-segregated with the SSLB resistance gene Rsp2 in the Foster/CIho 4780 F2 population. Southern hybridization analysis with DNA from the wheat/barley addition lines localized E-ACT/M-CAA-170 on the short arm of the barley chromosome 5(1H). Restriction fragment length polymorphism analysis with DNA clones previously mapped to the short arm of chromosome 5(1H) placed Rsp2 at a position flanked by the markers Act8 and ksuD14. A sequence-characterized amplified region (SCAR) marker (E-ACT/M-CAA-170a) was developed that co-segregated with not only Rsp2 in the Foster/CIho 4780 population but also resistance gene Rsp3 in the Foster/CIho 10644 population. This result indicates that Rsp3 is closely linked to Rsp2 on the short arm of chromosome 5(1H). The utility of SCAR marker E-ACT/M-CAA-170a for selecting Rsp2 in two different breeding populations was validated.
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sources of resistance to Septoria speckled leaf blotch caused by Septoria passerinii in barley
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie, 2004Co-Authors: Hala Toubiarahme, Brian J SteffensonAbstract:Septoria speckled leaf blotch (SSLB), incited by Septoria passerinii, has reemerged as one of the most serious foliar diseases of barley (Hordeum vulgare) in the Upper Midwest region of the United States over the last decade. The most cost-effective and environmentally safe method of preventing SSLB epidemics is through the use of resistant cultivars. Thus, the objective of this study was to investigate sources of resistance to S. passerinii in barley and to determine the reliability of greenhouse seedling tests for predicting the adult-plant reaction in the field. From a preliminary greenhouse screening of over 250 barley accessions, 78 lines were selected and subsequently evaluated at the seedling (greenhouse) and adult-plant (field) stages for reaction to S. passerinii. All of the major malting (H. vulgare ‘Drummond’, ‘Excel’, ‘Foster’, ‘Lacey’, ‘Legacy’, ‘Morex’, ‘Stander’, ‘Conlon’, and ‘Robust’) and feed (H. vulgare ‘Bowman’, ‘Logan’, and ‘Royal’) cultivars grown in or recommended for the Upper Midw...
Afdhal Afdhal - One of the best experts on this subject based on the ideXlab platform.
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pendeteksian Septoria pada tanaman tomat dengan metode deep learning berbasis raspberry pi
Jurnal RESTI (Rekayasa Sistem dan Teknologi Informasi), 2021Co-Authors: Kahlil Muchtar, Yudha Nurdin, Afdhal AfdhalAbstract:much needed to meet the needs of both industry and households. However, tomato plants still require serious handling in increasing the yields. Data from the Central Bureau of Statistics shows that the number of tomatoes produced is not in accordance with a large number of market demands, resulting from the decrease of tomato yields. One of the obstacles in increasing tomato production is that the crops are attacked by Septoria leaf spot disease due to the fungus or the fungus Septoria Lycopersici Speg. Most farmers have limited knowledge of the early symptoms, which are not obvious, and also facing difficulty in detecting this disease earlier. The problem has been causing disadvantages such as crop failure or plant death. Based on this problem, a study will be conducted with the aim of designing a tool that can be used to detect Septoria leaf spot disease based on deep learning using the Convolutional Neural Network (ConvNets or CNN) model, where an algorithm that resembles human nerves is one of the supervised learning and widely used for solving linear and non-linear problems. In addition, the researcher used the Raspberry Pi as a microcontroller and used the Intel Movidius Neural Computing Stick (NCS) which functions to speed up the computing process so that the detection process is easier because of its portable, fast and accurate nature. The average accuracy rate is 95.89% with detection accuracy between 84.22% to 100%.
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Pendeteksian Septoria pada Tanaman Tomat dengan Metode Deep Learning berbasis Raspberry Pi
'Ikatan Ahli Informatika Indonesia (IAII)', 2021Co-Authors: Muchtar Kahlil, Yudha Nurdin, Afdhal AfdhalAbstract:much needed to meet the needs of both industry and households. However, tomato plants still require serious handling in increasing the yields. Data from the Central Bureau of Statistics shows that the number of tomatoes produced is not in accordance with a large number of market demands, resulting from the decrease of tomato yields. One of the obstacles in increasing tomato production is that the crops are attacked by Septoria leaf spot disease due to the fungus or the fungus Septoria Lycopersici Speg. Most farmers have limited knowledge of the early symptoms, which are not obvious, and also facing difficulty in detecting this disease earlier. The problem has been causing disadvantages such as crop failure or plant death. Based on this problem, a study will be conducted with the aim of designing a tool that can be used to detect Septoria leaf spot disease based on deep learning using the Convolutional Neural Network (ConvNets or CNN) model, where an algorithm that resembles human nerves is one of the supervised learning and widely used for solving linear and non-linear problems. In addition, the researcher used the Raspberry Pi as a microcontroller and used the Intel Movidius Neural Computing Stick (NCS) which functions to speed up the computing process so that the detection process is easier because of its portable, fast and accurate nature. The average accuracy rate is 95.89% with detection accuracy between 84.22% to 100%. Salah satu komoditas hortikultura sayuran yang memiliki potensi untuk dikembangkan adalah tanaman tomat, karena memiliki nilai ekonomis yang tinggi, juga banyak dibutuhkan dalam memenuhi kebutuhan baik dalam industri maupun rumah tangga. Namun, tanaman tomat masih memerlukan penanganan serius dalam meningkatkan hasil. Data dari Badan Pusat Statistik menunjukkan bahwa jumlah tomat yang diproduksi tidak sesuai dengan banyaknya permintaan pasar, hal ini karena hasil produksi tomat menurun. Salah satu kendala dalam peningkatan hasil produksi tomat yaitu, tanaman terserang penyakit bercak daun Septoria akibat cendawan atau jamur Septoria Lycopersici Speg. Keterbatasan pengetahuan petani akan gejala awal yang tidak tampak jelas dan sukarnya mendeteksi secara cepat terhadap penyakit ini, menyebabkan kerugian seperti; gagal panen ataupun matinya tumbuhan. Berdasarkan masalah tersebut, maka akan dilaksanakan sebuah penelitian dengan tujuan merancang alat yang dapat digunakan untuk mendeteksi penyakit bercak daun Septoria berbasis pembelajaran mendalam dengan menggunakan model Convolutional Neural Network (ConvNets atau CNN), dimana algoritma yang menyerupai saraf manusia ini merupakan salah satu pembelajaran terawasi dan banyak digunakan untuk pemecahan masalah linear maupun nonlinear. Selain itu, peneliti memanfaatkan Raspberry Pi sebagai mikrokontroler serta menggunakan Intel Movidius Neural Computing Stick (NCS) yang berfungsi untuk mempercepat proses komputasi sehingga proses pendeteksian lebih mudah karena sifatnya yang portable, cepat dan akurat. Tingkat akurasi rata-rata yang dihasilkan adalah 95.89% dengan akurasi perdeteksi antara 84.22% s/d 100%
James K M Brown - One of the best experts on this subject based on the ideXlab platform.
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mycosphaerella graminicola from genomics to disease control
Molecular Plant Pathology, 2011Co-Authors: Elizabeth S Orton, Sian Deller, James K M BrownAbstract:SUMMARY This Mycosphaerella graminicola pathogen profile covers recent advances in the knowledge of this ascomycete fungus and of the disease it causes, Septoria tritici blotch of wheat. Research on this pathogen has accelerated since publication of a previous pathogen profile in this journal in 2002. Septoria tritici blotch continues to have high economic importance and widespread global impact on wheat production. Taxonomy:Mycosphaerella graminicola (Fuckel) J. Schrot. In Cohn (anamorph: Septoria tritici Roberge in Desmaz.). Kingdom Fungi, Phylum Ascomycota, Class Loculoascomycetes (filamentous ascomycetes), Order Dothideales, Genus Mycosphaerella, Species graminicola. Host range: Bread and durum wheat (Triticum aestivum L. and T. turgidum ssp. durum L.). Disease symptoms: Initially leaves develop a chlorotic flecking, which is followed by the development of necrotic lesions which contain brown–black pycnidia. Necrosis causes a reduction in photosynthetic capacity and therefore affects grain yield. Disease control: The disease is primarily controlled by a combination of resistant cultivars and fungicides. Rapid advances in disease control, especially in resistance breeding, are opening up new opportunities for the management of the disease. Useful websites:http://genome.jgi-psf.org/Mycgr3/Mycgr3.home.html.
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chromosomal location of a gene for resistance to Septoria tritici blotch mycosphaerella graminicola in the hexaploid wheat synthetic 6x
Theoretical and Applied Genetics, 2001Co-Authors: L S Arraiano, A J Worland, C Ellerbrook, James K M BrownAbstract:Septoria tritici blotch, caused by the fungus Mycosphaerella graminicola,is currently the major foliar disease of wheat world-wide, and new sources of resistance and knowledge about the genetics of resistance are needed to improve breeding for resistance to this disease. Sears’s ’Synthetic 6x’ hexaploid wheat, derived from a hybrid of Triticum dicoccoides and Triticum tauschii, was resistant to 12 of 13 isolates of M. graminicola tested. Chromosome 7D of ’Synthetic 6x’ was identified as carrying resistance to all 12 isolates in tests of seedlings of inter-varietal chromosome substitution lines of ’Synthetic 6x’ into ’Chinese Spring’ and to two isolates in tests of adult plants. A Septoria tritici blotch resistance gene, named Stb5, was identified using the M. graminicola isolate IPO94269 and mapped on the short arm of chromosome 7D, near the centromere, in a population of single homozygous chromosome-recombinant lines for the 7D chromosome.
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resistance of wheat cultivars and breeding lines to Septoria tritici blotch caused by isolates of mycosphaerella graminicola in field trials
Plant Pathology, 2001Co-Authors: James K M Brown, G H J Kema, Hansrudolf Forrer, E C P Verstappen, L S Arraiano, P A Brading, E M Foster, Padruot Men Fried, Eveline JennyAbstract:Isolate-specific resistance of 71 cultivars and breeding lines of wheat (Triticum aestivum) to Septoria tritici blotch was evaluated in six field trials in the Netherlands, Switzerland and the UK between 1995 and 1997. Each plot was inoculated with one of six single-pycnidium isolates of the pathogen Mycosphaerella graminicola. There were strong interactions between wheat lines and M. graminicola and the line-by-isolate interactions were stable over the six trials. Lines with specific resistance or specific susceptibility to each of the isolates were identified. Specific resistance to isolate IPO323 was especially common, being carried by 22 lines from 10 countries. The results confirm that line-by-isolate interactions in Septoria tritici blotch of wheat are effective in adult plants in field conditions, and are not simply confined to seedlings. Wheat lines with good, quantitative resistance to all or most isolates were identified, including lines from Brazil, the USA and seven European countries. These may be useful as sources of resistance in wheat breeding.
Hala Toubiarahme - One of the best experts on this subject based on the ideXlab platform.
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molecular mapping and marker assisted selection of genes for Septoria speckled leaf blotch resistance in barley
Phytopathology, 2006Co-Authors: Shaobin Zhong, Hala Toubiarahme, Brian J Steffenson, Kevin P SmithAbstract:Zhong, S., Toubia-Rahme, H., Steffenson, B. J., and Smith, K. P. 2006. Molecular mapping and marker-assisted selection of genes for Septoria speckled leaf blotch resistance in barley. Phytopathology 96:993-999. Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii, has emerged as one of the most important foliar diseases of barley in the Upper Midwest region of the United States. To map and tag genes for SSLB resistance, we developed two populations derived from the resistant accessions CIho 4780 and CIho 10644 and the susceptible malting cv. Foster. Segregation analysis of F2 plants or F2:3 families from the Foster/CIho 4780 and Foster/CIho 10644 populations revealed that a single dominant gene conferred resistance at the seedling stage. Bulked segregant analysis identified an amplified fragment length polymorphism marker, E-ACT/M-CAA-170, that co-segregated with the SSLB resistance gene Rsp2 in the Foster/CIho 4780 F2 population. Southern hybridization analysis with DNA from the wheat/barley addition lines localized E-ACT/M-CAA-170 on the short arm of the barley chromosome 5(1H). Restriction fragment length polymorphism analysis with DNA clones previously mapped to the short arm of chromosome 5(1H) placed Rsp2 at a position flanked by the markers Act8 and ksuD14. A sequence-characterized amplified region (SCAR) marker (E-ACT/M-CAA-170a) was developed that co-segregated with not only Rsp2 in the Foster/CIho 4780 population but also resistance gene Rsp3 in the Foster/CIho 10644 population. This result indicates that Rsp3 is closely linked to Rsp2 on the short arm of chromosome 5(1H). The utility of SCAR marker E-ACT/M-CAA-170a for selecting Rsp2 in two different breeding populations was validated.
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sources of resistance to Septoria speckled leaf blotch caused by Septoria passerinii in barley
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie, 2004Co-Authors: Hala Toubiarahme, Brian J SteffensonAbstract:Septoria speckled leaf blotch (SSLB), incited by Septoria passerinii, has reemerged as one of the most serious foliar diseases of barley (Hordeum vulgare) in the Upper Midwest region of the United States over the last decade. The most cost-effective and environmentally safe method of preventing SSLB epidemics is through the use of resistant cultivars. Thus, the objective of this study was to investigate sources of resistance to S. passerinii in barley and to determine the reliability of greenhouse seedling tests for predicting the adult-plant reaction in the field. From a preliminary greenhouse screening of over 250 barley accessions, 78 lines were selected and subsequently evaluated at the seedling (greenhouse) and adult-plant (field) stages for reaction to S. passerinii. All of the major malting (H. vulgare ‘Drummond’, ‘Excel’, ‘Foster’, ‘Lacey’, ‘Legacy’, ‘Morex’, ‘Stander’, ‘Conlon’, and ‘Robust’) and feed (H. vulgare ‘Bowman’, ‘Logan’, and ‘Royal’) cultivars grown in or recommended for the Upper Midw...
Shaobin Zhong - One of the best experts on this subject based on the ideXlab platform.
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molecular mapping and marker assisted selection of genes for Septoria speckled leaf blotch resistance in barley
Phytopathology, 2006Co-Authors: Shaobin Zhong, Hala Toubiarahme, Brian J Steffenson, Kevin P SmithAbstract:Zhong, S., Toubia-Rahme, H., Steffenson, B. J., and Smith, K. P. 2006. Molecular mapping and marker-assisted selection of genes for Septoria speckled leaf blotch resistance in barley. Phytopathology 96:993-999. Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii, has emerged as one of the most important foliar diseases of barley in the Upper Midwest region of the United States. To map and tag genes for SSLB resistance, we developed two populations derived from the resistant accessions CIho 4780 and CIho 10644 and the susceptible malting cv. Foster. Segregation analysis of F2 plants or F2:3 families from the Foster/CIho 4780 and Foster/CIho 10644 populations revealed that a single dominant gene conferred resistance at the seedling stage. Bulked segregant analysis identified an amplified fragment length polymorphism marker, E-ACT/M-CAA-170, that co-segregated with the SSLB resistance gene Rsp2 in the Foster/CIho 4780 F2 population. Southern hybridization analysis with DNA from the wheat/barley addition lines localized E-ACT/M-CAA-170 on the short arm of the barley chromosome 5(1H). Restriction fragment length polymorphism analysis with DNA clones previously mapped to the short arm of chromosome 5(1H) placed Rsp2 at a position flanked by the markers Act8 and ksuD14. A sequence-characterized amplified region (SCAR) marker (E-ACT/M-CAA-170a) was developed that co-segregated with not only Rsp2 in the Foster/CIho 4780 population but also resistance gene Rsp3 in the Foster/CIho 10644 population. This result indicates that Rsp3 is closely linked to Rsp2 on the short arm of chromosome 5(1H). The utility of SCAR marker E-ACT/M-CAA-170a for selecting Rsp2 in two different breeding populations was validated.
