The Experts below are selected from a list of 253623 Experts worldwide ranked by ideXlab platform
Hisako Gondo Higashi - One of the best experts on this subject based on the ideXlab platform.
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vero cell rabies vaccine produced using serum free Medium
Vaccine, 2004Co-Authors: N M Frazattigallina, Regina Maria Mouraofuches, Rosana L Paoli, Maria L N Silva, Cosue Miyaki, Elizabeth Juliana Ghiuro Valentini, Hisako Gondo HigashiAbstract:Abstract A new rabies vaccine was developed from Vero cells adhered to microcarriers, cultivated in a bioreactor in Serum-Free Medium and infected with the PV/VERO-Paris rabies virus strain. The viral suspensions were concentrated by tangential filtration, purified by chromatography and inactivated with β-propiolactone. In immunogenicity studies performed in mice immunized with three doses of the new vaccine (seven batches) and the commercial Verorab and HDCV, mean titers of neutralizing antibodies of 10.3–34.6, 6.54 and 9.36 IU/ml were found, respectively. The vaccine presented stability during 14 months at 2–8 °C, 30 days at 37 °C and 8 h at 45 °C. The use of Serum-Free Medium facilitated the downstream process leading to residual cellular DNA values
Shen Zunli - One of the best experts on this subject based on the ideXlab platform.
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Establishment of Schwann cell culture with a Serum-Free Medium at rats of newborn and adult age
Chinses Journal of Hand Surgery, 2001Co-Authors: Shen ZunliAbstract:Objective To explore the possibility of establishment of newborn and adult rat Schwann cell culture with “M” serum free Medium. Methods Newborn (aged 1 ～ 3 days) and adult ( aged 3 ～ 4 months) Lewis rat sciatic nerves were harvested and Schwann cells were obtained after a differential adhesion and a three week in vitro Wallerian degeneration, respectively. Schwann cells were then incubated with the “M” serum free Medium for one week. The rates of purity and proliferation of cultured Schwann cells were assessed by S 100 and 5 bromo 2 deoxyuridine (BrdU) immunostaining. Results After one week of Schwann cell culture, the purity and the proliferation rates of newborn Schwann cells were 95 % and 73.8 %, and those of adult Schwann cells were 93 % and 38.3 %, respectively. Moreover, the DNA syntheses of most of contaminated fibroblasts were interrupted. Conclusions With the application of the “M” serum free Medium, the technique of Schwann cell culture was simplified and the contamination of fibroblasts was effectively controlled.
Tomoyoshi Komiya - One of the best experts on this subject based on the ideXlab platform.
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long term stability of vero cell derived inactivated japanese encephalitis vaccine prepared using serum free Medium
Vaccine, 2008Co-Authors: Hiroko Toriniwa, Tomoyoshi KomiyaAbstract:Abstract We established a method of producing a Vero cell-derived Japanese encephalitis vaccine using Serum-Free Medium, and tested its stability using various stabilizers during the inactivation process and storage at 4 °C and 28 °C. Similar to previously reported results of cell culture in serum-containing Medium, Vero cells were cultured in a Serum-Free Medium multiplied well, and the viral yield was successfully increased to about 10 9 PFU/ml. Following formalin-inactivation and purification via ethanol precipitation and sucrose density ultracentrifugation of the virus solution, the vaccine had the same quality as, and higher immunogenicity, the mouse brain-derived vaccine in current use. Testing of several stabilizers showed that the addition of 0.5% glycine during the virus inactivation process facilitated the maintenance of immunogenicity for a long period of time. Furthermore, the addition of 0.5% glycine and 1.0% sorbitol as vaccine stabilizers after purification led to the maintenance of immunogenicity for 1 year, not dependent on the storage temperature (4 °C or 28 °C). These results indicate that, in contrast to the current mouse brain-derived vaccine, the Vero cell-derived vaccine can be prepared using Serum-Free Medium containing no animal-derived components, and that the vaccine can be stored at room temperature by adding stabilizers, suggesting the possibility of producing room temperature-stable vaccines.
Lax R Devireddy - One of the best experts on this subject based on the ideXlab platform.
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A Serum-Free Medium formulation efficiently supports isolation and propagation of canine adipose-derived mesenchymal stem/stromal cells
PloS one, 2019Co-Authors: Lax R Devireddy, Zhuoming Liu, Rudell Screven, Michael J. Myers, Lynne BoxerAbstract:Medium containing Fetal Bovine Serum (FBS) provides a supportive environment for isolation and expansion of mesenchymal stromal/stem cells (MSCs); however, the inherent variability of FBS may contribute to inconsistencies in cell growth and yield between batches of stem cell products. For this reason, we set out to develop a Serum-Free Medium capable of supporting the in vitro expansion of MSCs. First a naive Serum-Free Medium was formulated by Sato’s approach. Once it was established that the naive Serum-Free Medium supported the expansion of canine adipose-derived MSCs (Ad-MSCs), the Serum-Free Medium was optimized by addition of growth factors. Combinations of growth factors were chosen and compared by their effect on cell proliferation and colony formation. Growth characteristics of canine adipose-derived MSCs cultured in the Serum-Free Medium were comparable to those cultured in standard FBS containing Medium. In addition, cell surface marker expression and differentiation potential of Serum-Free and FBS-based cultures were also comparable. However, a commercial Serum-Free Medium developed for human MSC culture did not support growth of canine Ad-MSCs. In summary, canine Ad-MSCs isolated and cultured in Serum-Free Medium retained the basic characteristics of MSCs cultured in FBS containing Medium.
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Characterization of Canine Adipose-Derived Mesenchymal Stromal/Stem Cells in Serum-Free Medium.
Tissue engineering. Part C Methods, 2018Co-Authors: Zhuoming Liu, Rudell Screven, Lynne Boxer, Michael J. Myers, Lax R DevireddyAbstract:In this article, we report on the development of a defined Serum-Free Medium capable of supporting the culture expansion of mesenchymal stromal/stem cells (MSCs) from canine adipose tissue (canine Ad-MSCs). The potential benefits of Serum-Free media can only be utilized if cells cultured in Serum-Free media maintain the same functional characteristics as cells cultured in serum-containing media. Therefore, we analyze the characteristics of canine Ad-MSCs cultured in this Serum-Free Medium or in serum-containing Medium through evaluation of growth kinetics, clonogenic capacity, senescence, and differentiation capacity. Both, serum-containing Medium and our Serum-Free Medium, supported efficient growth and colony formation of canine Ad-MSCs. In addition, canine Ad-MSCs cultured in both media demonstrated similar viability after freeze/thaw, similar cell surface marker expression, and were capable of trilineage differentiation. While canine Ad-MSCs cultured in both media were generally similar, under the conditions of our study, canine Ad-MSCs cultured in Serum-Free Medium demonstrated a shorter lag phase and higher colony-forming capacity, accelerated population doubling, maintained multipotentiality at higher passage numbers, and underwent senescence at higher passage numbers compared to canine Ad-MSCs cultured in conventional serum-containing Medium. These results suggest that canine Ad-MSCs cultured in Serum-Free Medium retain the basic characteristics associated with canine Ad-MSCs cultured in serum-containing Medium, although some differences in growth kinetics were observed.
Lynne Boxer - One of the best experts on this subject based on the ideXlab platform.
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A Serum-Free Medium formulation efficiently supports isolation and propagation of canine adipose-derived mesenchymal stem/stromal cells
PloS one, 2019Co-Authors: Lax R Devireddy, Zhuoming Liu, Rudell Screven, Michael J. Myers, Lynne BoxerAbstract:Medium containing Fetal Bovine Serum (FBS) provides a supportive environment for isolation and expansion of mesenchymal stromal/stem cells (MSCs); however, the inherent variability of FBS may contribute to inconsistencies in cell growth and yield between batches of stem cell products. For this reason, we set out to develop a Serum-Free Medium capable of supporting the in vitro expansion of MSCs. First a naive Serum-Free Medium was formulated by Sato’s approach. Once it was established that the naive Serum-Free Medium supported the expansion of canine adipose-derived MSCs (Ad-MSCs), the Serum-Free Medium was optimized by addition of growth factors. Combinations of growth factors were chosen and compared by their effect on cell proliferation and colony formation. Growth characteristics of canine adipose-derived MSCs cultured in the Serum-Free Medium were comparable to those cultured in standard FBS containing Medium. In addition, cell surface marker expression and differentiation potential of Serum-Free and FBS-based cultures were also comparable. However, a commercial Serum-Free Medium developed for human MSC culture did not support growth of canine Ad-MSCs. In summary, canine Ad-MSCs isolated and cultured in Serum-Free Medium retained the basic characteristics of MSCs cultured in FBS containing Medium.
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Characterization of Canine Adipose-Derived Mesenchymal Stromal/Stem Cells in Serum-Free Medium.
Tissue engineering. Part C Methods, 2018Co-Authors: Zhuoming Liu, Rudell Screven, Lynne Boxer, Michael J. Myers, Lax R DevireddyAbstract:In this article, we report on the development of a defined Serum-Free Medium capable of supporting the culture expansion of mesenchymal stromal/stem cells (MSCs) from canine adipose tissue (canine Ad-MSCs). The potential benefits of Serum-Free media can only be utilized if cells cultured in Serum-Free media maintain the same functional characteristics as cells cultured in serum-containing media. Therefore, we analyze the characteristics of canine Ad-MSCs cultured in this Serum-Free Medium or in serum-containing Medium through evaluation of growth kinetics, clonogenic capacity, senescence, and differentiation capacity. Both, serum-containing Medium and our Serum-Free Medium, supported efficient growth and colony formation of canine Ad-MSCs. In addition, canine Ad-MSCs cultured in both media demonstrated similar viability after freeze/thaw, similar cell surface marker expression, and were capable of trilineage differentiation. While canine Ad-MSCs cultured in both media were generally similar, under the conditions of our study, canine Ad-MSCs cultured in Serum-Free Medium demonstrated a shorter lag phase and higher colony-forming capacity, accelerated population doubling, maintained multipotentiality at higher passage numbers, and underwent senescence at higher passage numbers compared to canine Ad-MSCs cultured in conventional serum-containing Medium. These results suggest that canine Ad-MSCs cultured in Serum-Free Medium retain the basic characteristics associated with canine Ad-MSCs cultured in serum-containing Medium, although some differences in growth kinetics were observed.
