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Bee Wah Lee - One of the best experts on this subject based on the ideXlab platform.

  • Copyright @ 2008 World Allergy Organization. Unauthorized reproduction of this article is prohibited. Food AllergyVLessons From Asia
    2016
    Co-Authors: Bee Wah Lee, Irvin Gerez, Lynette Pei-chi Shek, Shu E Soh, Hugo P. Van Bever
    Abstract:

    Objective: This is a review on published data available on food Allergy in East Asia and a discussion on the insights that it offers. Methods: PubMed searches were made for terms food Allergy and anaphylaxis, in combination with Asia. Results: There is a paucity of population-based prevalence studies on food Allergy in Asia. Certain unique food allergens, such as buck-wheat, chestnuts, chickpeas, bird’s nest, and royal jelly, which are consumed extensively by certain Asian populations have resulted in clinical food Allergy of little importance in other populations. Crustacean Shellfish is of importance in this region relative to other common food allergens. The high consumption of these foods and possibly coupled with cross-reactive tropomyosins from dominant inhalant dust mite and cockroach allergens in this region may explain this phenomenon. In contrast, the prevalence of peanut Allergy is relatively low in this region. The reasons for this difference are not apparent. However, this may be a reflection of the general reduced propensity in this region to allergic diseases as seen with asthma. Conclusions: Further research on food Allergy in Asia is warranted because it offers unique opportunities to further our understanding on the influence of population and environment. Key Words: food Allergy, Asia, Shellfish Allergy, peanut Allergy, buckwheat, bird’s nest, chickpeas, royal jell

  • Shellfish and house dust mite allergies is the link tropomyosin
    Allergy Asthma and Immunology Research, 2016
    Co-Authors: Lydia Helena Wong, Bee Wah Lee, Chiunghui Huang
    Abstract:

    Crustacean Shellfish Allergy is an important cause of food Allergy and anaphylaxis in Asia. The major allergen in Shellfish Allergy is tropomyosin, a pan-allergen that is also found in house dust mites and cockroaches. Tropomyosins from house dust mites (HDMs) have a high sequence homology to Shellfish tropomyosins, and cross-reactivity between HDM and shrimp tropomyosins has been demonstrated. Exposure to inhaled tropomyosins from house dust mites has been postulated to be the primary sensitizer for Shellfish Allergy, in a reaction analogous to the oral Allergy (inhalant-food) syndrome. This notion is supported by indirect data from the effects of HDM immunotherapy on Shellfish Allergy, and strong correlations of Shellfish and HDM sensitization. HDM immunotherapy has been reported to induce both shrimp Allergy in non-allergic patients and shrimp tolerance in shrimp-allergic patients. Epidemiological surveys have also demonstrated a strong correlation between Shellfish and HDM sensitization in both hospital-based and community-based studies. Unexposed populations have also been shown to develop sensitization-Shellfish sensitization in orthodox Jews with no history of Shellfish consumption was associated with HDM sensitization. Reciprocally, HDM sensitization in an Icelandic population living in a HDM-free environment was associated with shrimp sensitization. In vitro IgE inhibition studies on sera in shrimp-allergic Spanish patients indicate that mites are the primary sensitizer in shrimp-allergic patients living in humid and warm climates. Current data supports the hypothesis that tropomyosin is the link between HDM and Shellfish allergies. The role of tropomyosin in HDM and Shellfish allergies is a fertile field for investigation as it may provide novel immunotherapeutic strategies for Shellfish Allergy.

  • A population-based questionnaire survey on the prevalence of peanut, tree nut, and Shellfish Allergy in 2 Asian populations
    Journal of Allergy and Clinical Immunology, 2010
    Co-Authors: Lynette Pei-chi Shek, Irvin Gerez, Elizabeth Ann Cabrera-morales, Shu E Soh, Bee Wah Lee
    Abstract:

    Background There has been a substantial increase in the prevalence of peanut and tree nut Allergy in Western populations in the last 2 decades. However, there is an impression that peanut and tree nut Allergy is relatively uncommon in Asia. Objective To evaluate the prevalence of peanut, tree nut, and Shellfish Allergy in schoolchildren in 2 Asian countries (Singapore and Philippines). Methods A structured written questionnaire was administered to local and expatriate Singapore (4-6 and 14-16 years old) and Philippine (14-16 years old) schoolchildren. Results A total of 25,692 schoolchildren responded to the survey (response rate, 74.2%). Of these, 23,425 responses fell within the study protocol's 4 to 6 and 14 to 16 year age groups and were included in the analysis. The prevalence of convincing peanut and tree nut Allergy were similar in both local Singapore (4-6 years, 0.64%, 0.28%; 14-16 years, 0.47%, 0.3%, respectively) and Philippine (14-16, 0.43%, 0.33%, respectively) schoolchildren, but was higher in the Singapore expatriates (4-6 years, 1.29%, 1.12%; 14-16 years, both 1.21%, respectively; 4-6 years, expatriates vs local Singaporeans: peanut, P  = .019; tree nut, P  = .0017; 14-16 years, P  > .05). Conversely, Shellfish Allergy was more common in the local Singapore (4-6 years, 1.19%; 14-16 years, 5.23%) and Philippine (14-16 years, 5.12%) schoolchildren compared with expatriate children (4-6 years, 0.55%; 14-16 years, 0.96%; P Conclusion This study substantiates the notion that peanut and tree nut Allergy is relatively low in Asian children, and instead Shellfish Allergy predominates. Environmental factors that are yet to be defined are likely to contribute to these differences.

Pascal Capdevila I Mariona - One of the best experts on this subject based on the ideXlab platform.

  • Allergenic protein and epitope recognition in food Allergy: a new perspective for the molecular and clinical characterization of Shellfish and lipid transfer protein Allergy / Reconeixement de proteïnes i epítops al•lergènics en al•lèrgia alimentaria: una nova perspectiva per a la caracterització clínica i molecular de l’al•lèrgia al marisc i a les proteïnes de transferència de lípids
    'Edicions de la Universitat de Barcelona', 2012
    Co-Authors: Pascal Capdevila I Mariona
    Abstract:

    Currently food Allergy diagnostic tests are not able to predict clinical reactivity in sensitized patients (those with specific IgE against a particular allergen). Traditionally, Allergy diagnostic tests used complete extracts of allergenic sources containing multiple molecules, some allergic and others not. This greatly limits the accuracy in the diagnosis and identification of possible allergic reactions to different foods by the existence of cross reactivity. Through the last decades, thanks to advances in the characterization of allergens at molecular level, the concept of Component-Based Diagnosis has been developed. This is based on the reasoning that the presence of specific IgE for the protein actually responsible for the allergic response should be detected and not the one against a mixture of molecules. Furthermore, the study of IgE and IgG4 recognition at epitope level using microarrays of synthetic peptides has been described as a useful tool for diagnosis, prognosis and development of a therapy for food Allergy. The hypothesis of this thesis is that these new methodologies can improve the diagnosis of Shellfish and lipid transfer protein (LTP) Allergy. The aim of this thesis is to characterize clinically and at a molecular level these two types of food allergies, using these new methodologies. Regarding Shellfish Allergy, we found that tropomyosin, sarcoplasmic protein binding of calcium and myosin light chain are the allergens associated with clinical reactivity, i.e, are more common in shrimp allergic patients than in tolerant individuals sensitized shrimp. On the other hand, arginine kinase and hemocyanin allergens would be more involved in the phenomena of cross-reactivity with other arthropods (mites and/or cockroach). Additionally the synthetic peptide microarray has identified differential recognition of IgE and IgG4 epitopes among allergic and tolerant individuals. Regarding Allergy to LTP, allergenic proteins ubiquitously distributed in the plant kingdom, we observed that patients suffer from reactions with a wide range of plant foods to which are sensitized, being peach the most common one. Furthermore, these patients have a variety of clinical symptoms from very mild to very severe and life-threatening as in the case of anaphylaxis, which can be attributed to allergens from different families. The component-based diagnosis in a microarray format that includes a diverse panel of allergenic proteins from different families is useful for diagnosing these patients, since the only proteins identified as responsible for the clinical symptoms are the LTPs, although the symptoms are diverse and sometimes more closely resemble to those caused by other allergens such as profilins or homologues of Bet v 1. Moreover, it offers an overview of positive and negative sensitivities in these patients in a single trial, with its multiplex properties. Cases of anaphylaxis with a cofactor involvement, such as NSAIDs, were frequently observed in these patients. The simultaneous presence of these drugs with the food allergen triggers allergic reactions in the individual that would not occur without the presence of the drug or would have been of less severity. We have developed a preliminary in vitro model based on the basophil activation test that allowed us to observe the in vitro effect observed in vivo. We observed an increase of degranulation/activation of basophils when stimulation is done with food in the presence the drug, compared to when stimulated only with food. In this thesis we can conclude that both the component-based diagnosis and epitope mapping are useful tools for the characterization of food Allergy to Shellfish proteins and LTP, and that they should be considered to improve the efficiency of diagnosis of these two types of food allergies.Actualment els mètodes diagnòstics de l'al•lèrgia alimentària no són capaços de predir la reactivitat clínica dels pacients sensibilitzats (els que tenen IgE específica davant un determinat al•lergen). Tradicionalment les proves diagnòstiques de l'al•lèrgia han utilitzat extractes complets de fonts al•lergèniques que contenen múltiples molècules, algunes al•lergèniques i altres no. Això limita enormement la precisió en el diagnòstic i la possibilitat d'identificar reaccions al•lèrgiques a diferents aliments per l'existència de reactivitats creuades. Gràcies a la caracterització dels al•lèrgens a nivell molecular, s'ha desenvolupat el concepte del Diagnòstic Basat en Components que es basa en el raonament de detectar la presència d'IgE específica per a la proteïna realment responsable de la resposta al•lèrgica i no per una mescla de molècules. Addicionalment, l'estudi del reconeixement IgE i IgG4 a nivell d'epítops amb microarrays de pèptids sintètics pot ser una eina útil per al diagnòstic, pronòstic i desenvolupament d'una teràpia per l'al•lèrgia alimentària. La hipòtesi d'aquesta tesi és que aquestes noves metodologies poden millorar el diagnòstic de l'al•lèrgia al marisc i a les proteïnes de transferència de lípids (LTP), presents en múltiples aliments vegetals. L'objectiu és doncs caracteritzar clínicament i a nivell molecular aquests dos tipus d'al lèrgies alimentàries, utilitzant aquestes noves metodologies. Respecte a l'al•lèrgia al marisc, els al lèrgens tropomiosina, proteïna sarcoplàsmica d'unió de calci i la cadena lleugera de miosina s'associen amb la reactivitat clínica a la gamba. D'altra banda, els al•lèrgens arginina quinasa i hemocianina estarien més implicats en fenòmens de reactivitat creuada amb altres artròpodes. Addicionalment, amb el microarray de pèptids sintètics s'ha pogut identificar un reconeixement diferencial d'epítops IgE i IgG4 entre pacients al•lèrgics i tolerants. Respecte a l'al•lèrgia a les LTP, els pacients pateixen reaccions amb un ampli ventall d'aliments vegetals, sent el préssec el més freqüent, amb una gran diversitat de símptomes clínics, que poden atribuir-se a al•lèrgens de diferents famílies. El diagnòstic basat en components en el format d'un microarray que inclou proteïnes al•lergèniques de diferents famílies és útil per al diagnòstic d'aquests pacients, ja que permet identificar que les úniques proteïnes responsables els símptomes clínics són les LTP, encara que els símptomes siguin molt variats i en algunes ocasions s'assemblin més als provocats per altres al•lèrgens com les profilines o els homòlegs de Bet v 1. En aquests pacients són freqüents els casos d'anafilàxia en què està involucrat un cofactor, com els antiinflamatoris no esteroïdals. La presència del fàrmac amb l'al•lergen alimentari desencadena reaccions al•lèrgiques que sense el fàrmac no es donarien o serien de menor severitat. Hem desenvolupat un model preliminar in vitro basat en el test d'activació de basòfils que ens ha permès observar in vitro l'efecte observat in vivo. En conclusió, el diagnòstic basat en components i el mapatge d'epítops són eines útils per a la caracterització de l'al•lèrgia alimentària al marisc i a les proteïnes LTP, i s'han de considerar per millorar l'eficiència del diagnòstic d'aquests dos tipus d'al•lèrgies alimentàries

  • Allergenic protein and epitope recognition in food Allergy: a new perspective for the molecular and clinical characterization of Shellfish and lipid transfer protein Allergy / Reconeixement de proteïnes i epítops al·lergènics en al·lèrgia alimentaria: una nova perspectiva per a la caracterització clínica i molecular de l’al·lèrgia al marisc i a les proteïnes de transferència de lípids
    'Edicions de la Universitat de Barcelona', 2012
    Co-Authors: Pascal Capdevila I Mariona
    Abstract:

    [eng] Currently food Allergy diagnostic tests are not able to predict clinical reactivity in sensitized patients (those with specific IgE against a particular allergen). Traditionally, Allergy diagnostic tests used complete extracts of allergenic sources containing multiple molecules, some allergic and others not. This greatly limits the accuracy in the diagnosis and identification of possible allergic reactions to different foods by the existence of cross reactivity. Through the last decades, thanks to advances in the characterization of allergens at molecular level, the concept of Component-Based Diagnosis has been developed. This is based on the reasoning that the presence of specific IgE for the protein actually responsible for the allergic response should be detected and not the one against a mixture of molecules. Furthermore, the study of IgE and IgG4 recognition at epitope level using microarrays of synthetic peptides has been described as a useful tool for diagnosis, prognosis and development of a therapy for food Allergy. The hypothesis of this thesis is that these new methodologies can improve the diagnosis of Shellfish and lipid transfer protein (LTP) Allergy. The aim of this thesis is to characterize clinically and at a molecular level these two types of food allergies, using these new methodologies. Regarding Shellfish Allergy, we found that tropomyosin, sarcoplasmic protein binding of calcium and myosin light chain are the allergens associated with clinical reactivity, i.e, are more common in shrimp allergic patients than in tolerant individuals sensitized shrimp. On the other hand, arginine kinase and hemocyanin allergens would be more involved in the phenomena of cross-reactivity with other arthropods (mites and/or cockroach). Additionally the synthetic peptide microarray has identified differential recognition of IgE and IgG4 epitopes among allergic and tolerant individuals. Regarding Allergy to LTP, allergenic proteins ubiquitously distributed in the plant kingdom, we observed that patients suffer from reactions with a wide range of plant foods to which are sensitized, being peach the most common one. Furthermore, these patients have a variety of clinical symptoms from very mild to very severe and life-threatening as in the case of anaphylaxis, which can be attributed to allergens from different families. The component-based diagnosis in a microarray format that includes a diverse panel of allergenic proteins from different families is useful for diagnosing these patients, since the only proteins identified as responsible for the clinical symptoms are the LTPs, although the symptoms are diverse and sometimes more closely resemble to those caused by other allergens such as profilins or homologues of Bet v 1. Moreover, it offers an overview of positive and negative sensitivities in these patients in a single trial, with its multiplex properties. Cases of anaphylaxis with a cofactor involvement, such as NSAIDs, were frequently observed in these patients. The simultaneous presence of these drugs with the food allergen triggers allergic reactions in the individual that would not occur without the presence of the drug or would have been of less severity. We have developed a preliminary in vitro model based on the basophil activation test that allowed us to observe the in vitro effect observed in vivo. We observed an increase of degranulation/activation of basophils when stimulation is done with food in the presence the drug, compared to when stimulated only with food. In this thesis we can conclude that both the component-based diagnosis and epitope mapping are useful tools for the characterization of food Allergy to Shellfish proteins and LTP, and that they should be considered to improve the efficiency of diagnosis of these two types of food allergies.[cat] Actualment els mètodes diagnòstics de l'al•lèrgia alimentària no són capaços de predir la reactivitat clínica dels pacients sensibilitzats (els que tenen IgE específica davant un determinat al•lergen). Tradicionalment les proves diagnòstiques de l'al•lèrgia han utilitzat extractes complets de fonts al•lergèniques que contenen múltiples molècules, algunes al•lergèniques i altres no. Això limita enormement la precisió en el diagnòstic i la possibilitat d'identificar reaccions al•lèrgiques a diferents aliments per l'existència de reactivitats creuades. Gràcies a la caracterització dels al•lèrgens a nivell molecular, s'ha desenvolupat el concepte del Diagnòstic Basat en Components que es basa en el raonament de detectar la presència d'IgE específica per a la proteïna realment responsable de la resposta al•lèrgica i no per una mescla de molècules. Addicionalment, l'estudi del reconeixement IgE i IgG4 a nivell d'epítops amb microarrays de pèptids sintètics pot ser una eina útil per al diagnòstic, pronòstic i desenvolupament d'una teràpia per l'al•lèrgia alimentària. La hipòtesi d'aquesta tesi és que aquestes noves metodologies poden millorar el diagnòstic de l'al•lèrgia al marisc i a les proteïnes de transferència de lípids (LTP), presents en múltiples aliments vegetals. L'objectiu és doncs caracteritzar clínicament i a nivell molecular aquests dos tipus d'al lèrgies alimentàries, utilitzant aquestes noves metodologies. Respecte a l'al•lèrgia al marisc, els al lèrgens tropomiosina, proteïna sarcoplàsmica d'unió de calci i la cadena lleugera de miosina s'associen amb la reactivitat clínica a la gamba. D'altra banda, els al•lèrgens arginina quinasa i hemocianina estarien més implicats en fenòmens de reactivitat creuada amb altres artròpodes. Addicionalment, amb el microarray de pèptids sintètics s'ha pogut identificar un reconeixement diferencial d'epítops IgE i IgG4 entre pacients al•lèrgics i tolerants. Respecte a l'al•lèrgia a les LTP, els pacients pateixen reaccions amb un ampli ventall d'aliments vegetals, sent el préssec el més freqüent, amb una gran diversitat de símptomes clínics, que poden atribuir-se a al•lèrgens de diferents famílies. El diagnòstic basat en components en el format d'un microarray que inclou proteïnes al•lergèniques de diferents famílies és útil per al diagnòstic d'aquests pacients, ja que permet identificar que les úniques proteïnes responsables els símptomes clínics són les LTP, encara que els símptomes siguin molt variats i en algunes ocasions s'assemblin més als provocats per altres al•lèrgens com les profilines o els homòlegs de Bet v 1. En aquests pacients són freqüents els casos d'anafilàxia en què està involucrat un cofactor, com els antiinflamatoris no esteroïdals. La presència del fàrmac amb l'al•lergen alimentari desencadena reaccions al•lèrgiques que sense el fàrmac no es donarien o serien de menor severitat. Hem desenvolupat un model preliminar in vitro basat en el test d'activació de basòfils que ens ha permès observar in vitro l'efecte observat in vivo. En conclusió, el diagnòstic basat en components i el mapatge d'epítops són eines útils per a la caracterització de l'al•lèrgia alimentària al marisc i a les proteïnes LTP, i s'han de considerar per millorar l'eficiència del diagnòstic d'aquests dos tipus d'al•lèrgies alimentàries

Hugh A. Sampson - One of the best experts on this subject based on the ideXlab platform.

  • sarcoplasmic calcium binding protein is an ef hand type protein identified as a new shrimp allergen
    The Journal of Allergy and Clinical Immunology, 2009
    Co-Authors: Rosalia Ayuso, Galina Grishina, Maria Dolores Ibanez, Carlos A Blanco, Teresa Carrillo, Ramon Bencharitiwong, Silvia Sanchez, Anna Nowakwegrzyn, Hugh A. Sampson
    Abstract:

    Background Shellfish Allergy is a long-lasting disorder usually persisting throughout life. Despite its high prevalence, there is limited information about allergenic shrimp proteins. Objective Characterization of shrimp allergens. Methods Fifty-two adults and children with a history of immediate allergic reactions to shrimp and elevated serum IgE to shrimp were selected for this study. Tryptic digests from a 20-kd IgE-binding protein were analyzed by LC-MS/MS, identifying the protein as a sarcoplasmic-calcium-binding protein. cDNA encoding sarcoplasmic calcium-binding protein (SCP) from a shrimp cDNA library ( Litopenaeus vannamei ) was amplified by PCR, cloned into an expression vector, and sequenced. Recombinant SCP was tested with patients' sera. ELISA inhibition experiments determined the fraction of total shrimp IgE recognizing SCP. A functional assay with a rat basophilic leukemia cell line was used to determine the capacity for mediator release induced by SCP. Results Immunoblotting demonstrated IgE binding by 31 of 52 (59.6%) of the sera to a 20-kd shrimp protein. The protein was identified as a SCP. Amplified cDNA encoding SCP was isolated and sequenced. Open reading frame translation provided the complete amino acid sequence of shrimp SCP. Recombinant SCP was recognized by serum IgE from 20 of 52 (38.4%) subjects, of whom 17 of 20 (85%) were children. ELISA inhibition of pooled sera IgE reactivity to BS extract using recombinant SCP was significant (as high as 79%). For some subjects, mediator release induced by recombinant SCP was higher than that induced by recombinant tropomyosin. Conclusion We have identified and cloned a new shrimp allergen, Lit v 4.0101, an SCP, which appears to be of particular importance in the pediatric population.

  • myosin light chain is a novel shrimp allergen lit v 3
    The Journal of Allergy and Clinical Immunology, 2008
    Co-Authors: Rosalia Ayuso, Galina Grishina, Maria Dolores Ibanez, Carlos A Blanco, Teresa Carrillo, Hugh A. Sampson, L Bardina, Kirsten Beyer
    Abstract:

    Background Shellfish Allergy is a prevalent, long-lasting disorder usually persisting throughout life. Few options are available for treatment, and avoidance is the only therapy recommended. Objective We sought to identify relevant crustacean allergens for use as diagnostic and safe immunotherapeutic agents for subjects with Shellfish Allergy. Methods Thirty-eight patients were selected with immediate allergic reactions to shrimp and increased shrimp-specific serum IgE levels. One-dimensional and 2-dimensional electrophoresis of shrimp extracts were followed by IgE immunoblotting. Protein identification was done with matrix-assisted laser desorption/ionization–mass spectrometry and Edman sequencing. A cDNA library was generated from white pacific shrimp (Litopenaeus vannamei) and screened with primers designed on the basis of internal sequences obtained from 2-dimensional tryptic digests. Full-length cDNA clones were isolated from the library and sequenced. Recombinant protein was expressed and tested with sera from patients with shrimp Allergy. Results Immunoblotting demonstrated IgE binding to a 20-kDa shrimp protein by 21 (55%) of 38 sera. Tryptic digestion of the protein followed by matrix-assisted laser desorption/ionization–mass spectrometric analysis and Edman sequencing identified it as a myosin light chain (MLC). Screening of the shrimp cDNA library resulted in isolation of a novel protein cDNA. Open reading frame translation provided the amino acid sequence of a new allergenic shrimp protein with high similarity to Bla g 8 (cockroach MLC). Recombinant protein was recognized by 17 patients, confirming the allergenicity of shrimp MLC. Conclusions We have identified and cloned a new major shrimp allergen, Lit v 3.0101, an MLC protein.

  • Prevalence of seafood Allergy in the United States determined by a random telephone survey
    The Journal of allergy and clinical immunology, 2004
    Co-Authors: Scott H. Sicherer, Anne Muñoz-furlong, Hugh A. Sampson
    Abstract:

    Abstract Background Seafood Allergy is potentially severe, but the prevalence of this group of food allergies in the US population has not been determined. Objective To estimate the prevalence of seafood (fish, Shellfish) Allergy in the United States. Methods We performed a nationwide, cross-sectional, random telephone survey by using a standardized questionnaire. Criteria were established in advance to define seafood Allergy by report of convincing symptoms and physician evaluation. Results A total of 5529 households completed the survey (67.3% participation rate), representing a census of 14,948 individuals. Fish or Shellfish Allergy defined by established criteria was reported in 5.9% (95% CI, 5.3%-6.6%) of households and among individuals as follows: 2.3% (95% CI, 2%-2.5%) for any seafood Allergy, 2% for Shellfish, 0.4% for fish, and 0.2% for both types. Seafood Allergy was more common in adults compared with children (2.8% vs 0.6%; P P Conclusions Physician-diagnosed and/or convincing seafood Allergy is reported by 2.3% of the general population, or approximately 6.6 million Americans. Affected individuals typically report recurrent and sometimes severe reactions, indicating that seafood Allergy represents a significant health concern.

Rueda García María - One of the best experts on this subject based on the ideXlab platform.

  • Diagnóstico de los pacientes con alergia a gamba
    'Universitat Autonoma de Barcelona', 2019
    Co-Authors: Rueda García María
    Abstract:

    Introducción: La alergia a marisco es una de las principales causas de alergia a alimentos en adultos, y es una causa frecuente de anafilaxia. Se estima que tiene una prevalencia mundial del 5.4%. Está establecido que la tropomiosina es el alérgeno mayor de la gamba, y se considera un panalérgeno, responsable de la gran reactividad cruzada entre crustáceos y ácaros del polvo doméstico. El perfil de sensibilización alergénica a la gamba de los pacientes puede ser diferente según sea el clima y la exposición a ácaros a los que se vean sometidos. En la actualidad el diagnóstico de alergia a gambas incluye la realización de pruebas intraepidérmicas, determinaciones de IgE específicas y pruebas de provocación oral controlada. Objetivos: El objetivo principal de este estudio fue evaluar la presencia de reactividad cruzada entre ácaros y gamba en nuestra población. Métodos: Se reclutaron pacientes con pruebas cutáneas positivas a gamba y se clasificaron en función de las manifestaciones clínicas. Se prepararon extractos alergénicos de varias especies de ácaros y gambas, y se cuantificó el contenido proteico. Se realizaron pruebas cutáneas intraepidérmicas y determinación de IgE específica por absorbancia a todos los extractos, y de IgE específica a gamba y Pen a 1 por enzimoinmunoensayo. Mediante SDS-PAGE y Western blot se obtuvieron los perfiles proteicos y alergénicos, y se realizaron ELISA de competición y Western blot de inhibición para valorar la reactividad cruzada entre ácaros y gamba. Resultados: De los 117 pacientes incorporados al estudio, 99 (84.6%) se clasificaron como alérgicos a gamba. Las principales manifestaciones clínicas fueron urticaria/angioedema (42.4%) y anafilaxia (34.4%). El 73.7% de los pacientes alérgicos a gamba también eran alérgicos a ácaros. En los extractos estudiados se detectaron varias proteínas comunes: tropomiosina, paramiosina, arginina quinasa, triosa-fosfato isomerasa y proteína de choque térmico. La determinación de IgE específica a gamba, a Pen a 1 y a los extractos nuevos no presentó diferencias estadísticamente significativas entre alérgicos y no alérgicos. Se demostró reactividad cruzada entre ácaros y gamba, siendo los ácaros el sensibilizador primario. Conclusión: Este es el primer estudio realizado en Barcelona que demuestra la existencia de reactividad cruzada entre los ácaros del polvo doméstico y las gambas, siendo los ácaros los responsables de la sensibilización primaria. En los extractos de ácaros del polvo doméstico y de gamba estudiados se han hallado proteínas comunes que podrían justificar la reactividad cruzada entre ambos. Las pruebas cutáneas intraepidérmicas a los extractos testados y la determinación de IgE específica a gamba y a Pen a 1, no son capaces de discriminar a los pacientes alérgicos a gamba de los no alérgicos.Background: Shellfish Allergy is one of the main causes of food Allergy in adults, and often causes anaphylaxis. It is estimated a global prevalence of 5.4%. Tropomyosin is the major allergen in shrimp and it is considered a cross-reactive panallergen, responsible of reactivity between crustaceans and mites. Exposure to mites can modify the divers allergenic profile to shrimp. Currently, shrimp Allergy diagnosis include skin prick tests, specific IgE determinations and oral food challenge. Objectives: The main aim of this study was to evaluate the presence of cross-reactivity between shrimp and house dust mites in our population. Methods: Patients with positive skin prick test to shrimp were recruited and classified into groups according to the clinics. Different allergenic extracts were prepared from shrimp and house dust mites, and its protein content was achieved. Skin prick tests and specific IgE to shrimp and Pen a 1 was measured. The allergenic profile was studied by means of SDS-PAGE and Western blot. Cross-reactivity was analysed by competition ELISA and Western blot. Results: 117 patients were enrolled, and 99 (84.6%) were classified as allergic to shrimp. The most reported symptoms were urticaria/angioedema (42.4%) and anaphylaxis (34.4%). 73.7% were also allergic to house dust mites. The newly prepared extracts had some proteins in common: tropomyosin, paramyosin, arginine kinase, triose phosphate isomerase and heat shock protein. Specific IgE to shrimp, to Pen a 1, and to the newly extracts did not showed statistical differences between allergic and non-allergic shrimp patients. Cross reactivity between shrimp and mites was demonstrated, with mites acting as a primary sensitizer. Conclusion: This is the first study performed in Barcelona that demonstrate the cross-reactivity between house dust mites and shrimp allergens. The primary sensitization was due to house dust mites. Cross reactivity could be explained because of the common proteins included in both extracts. Intraepidermal cutaneous test to these extracts, and specific IgE determinations to shrimp and to Pen a 1, fail to discern between shrimp allergic and non-allergic patients

  • Diagnóstico de los pacientes con alergia a gamba /
    2019
    Co-Authors: Rueda García María, Universitat Autònoma De Barcelona. Departament De Medicina
    Abstract:

    Departament responsable de la tesi: Departament de Medicina.Introducción: La alergia a marisco es una de las principales causas de alergia a alimentos en adultos, y es una causa frecuente de anafilaxia. Se estima que tiene una prevalencia mundial del 5.4%. Está establecido que la tropomiosina es el alérgeno mayor de la gamba, y se considera un panalérgeno, responsable de la gran reactividad cruzada entre crustáceos y ácaros del polvo doméstico. El perfil de sensibilización alergénica a la gamba de los pacientes puede ser diferente según sea el clima y la exposición a ácaros a los que se vean sometidos. En la actualidad el diagnóstico de alergia a gambas incluye la realización de pruebas intraepidérmicas, determinaciones de IgE específicas y pruebas de provocación oral controlada. Objetivos: El objetivo principal de este estudio fue evaluar la presencia de reactividad cruzada entre ácaros y gamba en nuestra población.Métodos: Se reclutaron pacientes con pruebas cutáneas positivas a gamba y se clasificaron en función de las manifestaciones clínicas. Se prepararon extractos alergénicos de varias especies de ácaros y gambas, y se cuantificó el contenido proteico. Se realizaron pruebas cutáneas intraepidérmicas y determinación de IgE específica por absorbancia a todos los extractos, y de IgE específica a gamba y Pen a 1 por enzimoinmunoensayo. Mediante SDS-PAGE y Western blot se obtuvieron los perfiles proteicos y alergénicos, y se realizaron ELISA de competición y Western blot de inhibición para valorar la reactividad cruzada entre ácaros y gamba. Resultados: De los 117 pacientes incorporados al estudio, 99 (84.6%) se clasificaron como alérgicos a gamba. Las principales manifestaciones clínicas fueron urticaria/angioedema (42.4%) y anafilaxia (34.4%). El 73.7% de los pacientes alérgicos a gamba también eran alérgicos a ácaros. En los extractos estudiados se detectaron varias proteínas comunes: tropomiosina, paramiosina, arginina quinasa, triosa-fosfato isomerasa y proteína de choque térmico. La determinación de IgE específica a gamba, a Pen a 1 y a los extractos nuevos no presentó diferencias estadísticamente significativas entre alérgicos y no alérgicos. Se demostró reactividad cruzada entre ácaros y gamba, siendo los ácaros el sensibilizador primario. Conclusión: Este es el primer estudio realizado en Barcelona que demuestra la existencia de reactividad cruzada entre los ácaros del polvo doméstico y las gambas, siendo los ácaros los responsables de la sensibilización primaria. En los extractos de ácaros del polvo doméstico y de gamba estudiados se han hallado proteínas comunes que podrían justificar la reactividad cruzada entre ambos. Las pruebas cutáneas intraepidérmicas a los extractos testados y la determinación de IgE específica a gamba y a Pen a 1, no son capaces de discriminar a los pacientes alérgicos a gamba de los no alérgicos.Background: Shellfish Allergy is one of the main causes of food Allergy in adults, and often causes anaphylaxis. It is estimated a global prevalence of 5.4%. Tropomyosin is the major allergen in shrimp and it is considered a cross-reactive panallergen, responsible of reactivity between crustaceans and mites. Exposure to mites can modify the divers allergenic profile to shrimp. Currently, shrimp Allergy diagnosis include skin prick tests, specific IgE determinations and oral food challenge. Objectives: The main aim of this study was to evaluate the presence of cross-reactivity between shrimp and house dust mites in our population. Methods: Patients with positive skin prick test to shrimp were recruited and classified into groups according to the clinics. Different allergenic extracts were prepared from shrimp and house dust mites, and its protein content was achieved. Skin prick tests and specific IgE to shrimp and Pen a 1 was measured. The allergenic profile was studied by means of SDS-PAGE and Western blot. Cross-reactivity was analysed by competition ELISA and Western blot. Results: 117 patients were enrolled, and 99 (84.6%) were classified as allergic to shrimp. The most reported symptoms were urticaria/angioedema (42.4%) and anaphylaxis (34.4%). 73.7% were also allergic to house dust mites. The newly prepared extracts had some proteins in common: tropomyosin, paramyosin, arginine kinase, triose phosphate isomerase and heat shock protein. Specific IgE to shrimp, to Pen a 1, and to the newly extracts did not showed statistical differences between allergic and non-allergic shrimp patients. Cross reactivity between shrimp and mites was demonstrated, with mites acting as a primary sensitizer. Conclusion: This is the first study performed in Barcelona that demonstrate the cross-reactivity between house dust mites and shrimp allergens. The primary sensitization was due to house dust mites. Cross reactivity could be explained because of the common proteins included in both extracts. Intraepidermal cutaneous test to these extracts, and specific IgE determinations to shrimp and to Pen a 1, fail to discern between shrimp allergic and non-allergic patients

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  • sarcoplasmic calcium binding protein is an ef hand type protein identified as a new shrimp allergen
    The Journal of Allergy and Clinical Immunology, 2009
    Co-Authors: Rosalia Ayuso, Galina Grishina, Maria Dolores Ibanez, Carlos A Blanco, Teresa Carrillo, Ramon Bencharitiwong, Silvia Sanchez, Anna Nowakwegrzyn, Hugh A. Sampson
    Abstract:

    Background Shellfish Allergy is a long-lasting disorder usually persisting throughout life. Despite its high prevalence, there is limited information about allergenic shrimp proteins. Objective Characterization of shrimp allergens. Methods Fifty-two adults and children with a history of immediate allergic reactions to shrimp and elevated serum IgE to shrimp were selected for this study. Tryptic digests from a 20-kd IgE-binding protein were analyzed by LC-MS/MS, identifying the protein as a sarcoplasmic-calcium-binding protein. cDNA encoding sarcoplasmic calcium-binding protein (SCP) from a shrimp cDNA library ( Litopenaeus vannamei ) was amplified by PCR, cloned into an expression vector, and sequenced. Recombinant SCP was tested with patients' sera. ELISA inhibition experiments determined the fraction of total shrimp IgE recognizing SCP. A functional assay with a rat basophilic leukemia cell line was used to determine the capacity for mediator release induced by SCP. Results Immunoblotting demonstrated IgE binding by 31 of 52 (59.6%) of the sera to a 20-kd shrimp protein. The protein was identified as a SCP. Amplified cDNA encoding SCP was isolated and sequenced. Open reading frame translation provided the complete amino acid sequence of shrimp SCP. Recombinant SCP was recognized by serum IgE from 20 of 52 (38.4%) subjects, of whom 17 of 20 (85%) were children. ELISA inhibition of pooled sera IgE reactivity to BS extract using recombinant SCP was significant (as high as 79%). For some subjects, mediator release induced by recombinant SCP was higher than that induced by recombinant tropomyosin. Conclusion We have identified and cloned a new shrimp allergen, Lit v 4.0101, an SCP, which appears to be of particular importance in the pediatric population.

  • myosin light chain is a novel shrimp allergen lit v 3
    The Journal of Allergy and Clinical Immunology, 2008
    Co-Authors: Rosalia Ayuso, Galina Grishina, Maria Dolores Ibanez, Carlos A Blanco, Teresa Carrillo, Hugh A. Sampson, L Bardina, Kirsten Beyer
    Abstract:

    Background Shellfish Allergy is a prevalent, long-lasting disorder usually persisting throughout life. Few options are available for treatment, and avoidance is the only therapy recommended. Objective We sought to identify relevant crustacean allergens for use as diagnostic and safe immunotherapeutic agents for subjects with Shellfish Allergy. Methods Thirty-eight patients were selected with immediate allergic reactions to shrimp and increased shrimp-specific serum IgE levels. One-dimensional and 2-dimensional electrophoresis of shrimp extracts were followed by IgE immunoblotting. Protein identification was done with matrix-assisted laser desorption/ionization–mass spectrometry and Edman sequencing. A cDNA library was generated from white pacific shrimp (Litopenaeus vannamei) and screened with primers designed on the basis of internal sequences obtained from 2-dimensional tryptic digests. Full-length cDNA clones were isolated from the library and sequenced. Recombinant protein was expressed and tested with sera from patients with shrimp Allergy. Results Immunoblotting demonstrated IgE binding to a 20-kDa shrimp protein by 21 (55%) of 38 sera. Tryptic digestion of the protein followed by matrix-assisted laser desorption/ionization–mass spectrometric analysis and Edman sequencing identified it as a myosin light chain (MLC). Screening of the shrimp cDNA library resulted in isolation of a novel protein cDNA. Open reading frame translation provided the amino acid sequence of a new allergenic shrimp protein with high similarity to Bla g 8 (cockroach MLC). Recombinant protein was recognized by 17 patients, confirming the allergenicity of shrimp MLC. Conclusions We have identified and cloned a new major shrimp allergen, Lit v 3.0101, an MLC protein.