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Haixia Zhao - One of the best experts on this subject based on the ideXlab platform.

  • a genetic transformation method for cadmium hyperaccumulator sedum plumbizincicola and non hyperaccumulating ecotype of sedum alfredii
    Frontiers in Plant Science, 2017
    Co-Authors: Huan Liu, Haixia Zhao
    Abstract:

    The present study demonstrates the development of an Agrobacterium-mediated genetic transformation method for species of the Sedum genus, which includes the Cd/Zn hyperaccumulator S. plumbizincicola and the non-hyperaccumulating ecotype of S. alfredii. Multiple Shoots were induced from stem nodes of two Sedum plants using Murashige and Skoog (MS) medium containing 0.1 mg/L cytokinin 6-benzyladenine (6-BA) and 1.0 mg/L auxin 1-naphthaleneacetic acid (NAA). The Shoot Primordia were used as direct targets for Agrobacterium infection. Selection on hygromycin was highly effective in generating Agrobacterium-transformed explants. This callus-free procedure allowed us to obtain transgenic plantlets after rooting hygromycin-resistant Shoots on phytohormone-free MS medium containing the antibiotic. The presence and expression of the reporter genes gusA and GFP in transgenic plants were confirmed by a real-time polymerase chain reaction (PCR), histochemical GUS assays, and confocal microscopy. This reliable method for genetic transformation of Sedum plants will help us to understand gene functions and the molecular mechanisms underlying Cd hypertolerance and hyperaccumulation in these species.

  • A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii
    Frontiers Media S.A., 2017
    Co-Authors: Huan Liu, Haixia Zhao
    Abstract:

    The present study demonstrates the development of an Agrobacterium-mediated genetic transformation method for species of the Sedum genus, which includes the Cd/Zn hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulating ecotype of S. alfredii. Multiple Shoots were induced from stem nodes of two Sedum plants using Murashige and Skoog (MS) medium containing 0.1 mg/L cytokinin 6-benzyladenine (6-BA) and 1.0 mg/L auxin 1-naphthaleneacetic acid (NAA). The Shoot Primordia were used as direct targets for Agrobacterium infection. Selection on hygromycin was highly effective in generating Agrobacterium-transformed explants. This callus-free procedure allowed us to obtain transgenic plantlets after rooting hygromycin-resistant Shoots on phytohormone-free MS medium containing the antibiotic. The presence and expression of the reporter genes gusA and GFP in transgenic plants were confirmed by a real-time polymerase chain reaction, histochemical GUS assays, and confocal microscopy. This reliable method for genetic transformation of Sedum plants will help us to understand gene functions and the molecular mechanisms underlying Cd hypertolerance and hyperaccumulation in these species

T. S. Rathore - One of the best experts on this subject based on the ideXlab platform.

  • Direct adventitious Shoot induction and plant regeneration of Embelia ribes Burm F.
    Plant Cell Tissue and Organ Culture (PCTOC), 2010
    Co-Authors: D. Annapurna, T. S. Rathore
    Abstract:

    An efficient micropropagation system via direct Shoot organogenesis from hypocotyl segments of Embelia ribes Burm F. was developed. A high frequency (84%) of adventitious Shoot induction was obtained on Murashige and Skoog (MS) medium supplemented with additives (283.85 μM ascorbic acid [AA], 118.96 μM citric acid [CA], 142.33 μM cysteine, and 684.22 μM glutamine) and 1.13 μM of thidiazuron (TDZ) after 4 weeks following culture. Further development of Shoot Primordia into well-grown Shoots of 4–5 cm in length was achieved by sub-culturing explants along with Shoot Primordia on MS medium supplemented with 0.44 μM benzyl adenine (BA) and 0.49 μM indole butyric acid (IBA) for three sub-culture periods with an interval of 15 days between them. The highest Shoot multiplication was obtained when explants were incubated on MS medium supplemented with 2.2 μM BA and 0.49 μM IBA in 4 weeks. All in vitro developed Shoots, 3–4 cm in length, rooted when grown on half-strength MS basal medium along with 2.47 μM IBA within 4 weeks. Moreover, 100% of Shoots developed roots when these were treated with 4.93 μM IBA for 20 min and then transferred to pots containing soilrite mix and grown in the greenhouse. In vitro and ex vitro rooted plants showed a survival of 85 and 95% respectively, during hardening in the greenhouse for a 6-week period.

Huan Liu - One of the best experts on this subject based on the ideXlab platform.

  • a genetic transformation method for cadmium hyperaccumulator sedum plumbizincicola and non hyperaccumulating ecotype of sedum alfredii
    Frontiers in Plant Science, 2017
    Co-Authors: Huan Liu, Haixia Zhao
    Abstract:

    The present study demonstrates the development of an Agrobacterium-mediated genetic transformation method for species of the Sedum genus, which includes the Cd/Zn hyperaccumulator S. plumbizincicola and the non-hyperaccumulating ecotype of S. alfredii. Multiple Shoots were induced from stem nodes of two Sedum plants using Murashige and Skoog (MS) medium containing 0.1 mg/L cytokinin 6-benzyladenine (6-BA) and 1.0 mg/L auxin 1-naphthaleneacetic acid (NAA). The Shoot Primordia were used as direct targets for Agrobacterium infection. Selection on hygromycin was highly effective in generating Agrobacterium-transformed explants. This callus-free procedure allowed us to obtain transgenic plantlets after rooting hygromycin-resistant Shoots on phytohormone-free MS medium containing the antibiotic. The presence and expression of the reporter genes gusA and GFP in transgenic plants were confirmed by a real-time polymerase chain reaction (PCR), histochemical GUS assays, and confocal microscopy. This reliable method for genetic transformation of Sedum plants will help us to understand gene functions and the molecular mechanisms underlying Cd hypertolerance and hyperaccumulation in these species.

  • A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii
    Frontiers Media S.A., 2017
    Co-Authors: Huan Liu, Haixia Zhao
    Abstract:

    The present study demonstrates the development of an Agrobacterium-mediated genetic transformation method for species of the Sedum genus, which includes the Cd/Zn hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulating ecotype of S. alfredii. Multiple Shoots were induced from stem nodes of two Sedum plants using Murashige and Skoog (MS) medium containing 0.1 mg/L cytokinin 6-benzyladenine (6-BA) and 1.0 mg/L auxin 1-naphthaleneacetic acid (NAA). The Shoot Primordia were used as direct targets for Agrobacterium infection. Selection on hygromycin was highly effective in generating Agrobacterium-transformed explants. This callus-free procedure allowed us to obtain transgenic plantlets after rooting hygromycin-resistant Shoots on phytohormone-free MS medium containing the antibiotic. The presence and expression of the reporter genes gusA and GFP in transgenic plants were confirmed by a real-time polymerase chain reaction, histochemical GUS assays, and confocal microscopy. This reliable method for genetic transformation of Sedum plants will help us to understand gene functions and the molecular mechanisms underlying Cd hypertolerance and hyperaccumulation in these species

D. Annapurna - One of the best experts on this subject based on the ideXlab platform.

  • Direct adventitious Shoot induction and plant regeneration of Embelia ribes Burm F.
    Plant Cell Tissue and Organ Culture (PCTOC), 2010
    Co-Authors: D. Annapurna, T. S. Rathore
    Abstract:

    An efficient micropropagation system via direct Shoot organogenesis from hypocotyl segments of Embelia ribes Burm F. was developed. A high frequency (84%) of adventitious Shoot induction was obtained on Murashige and Skoog (MS) medium supplemented with additives (283.85 μM ascorbic acid [AA], 118.96 μM citric acid [CA], 142.33 μM cysteine, and 684.22 μM glutamine) and 1.13 μM of thidiazuron (TDZ) after 4 weeks following culture. Further development of Shoot Primordia into well-grown Shoots of 4–5 cm in length was achieved by sub-culturing explants along with Shoot Primordia on MS medium supplemented with 0.44 μM benzyl adenine (BA) and 0.49 μM indole butyric acid (IBA) for three sub-culture periods with an interval of 15 days between them. The highest Shoot multiplication was obtained when explants were incubated on MS medium supplemented with 2.2 μM BA and 0.49 μM IBA in 4 weeks. All in vitro developed Shoots, 3–4 cm in length, rooted when grown on half-strength MS basal medium along with 2.47 μM IBA within 4 weeks. Moreover, 100% of Shoots developed roots when these were treated with 4.93 μM IBA for 20 min and then transferred to pots containing soilrite mix and grown in the greenhouse. In vitro and ex vitro rooted plants showed a survival of 85 and 95% respectively, during hardening in the greenhouse for a 6-week period.

H J M Dons - One of the best experts on this subject based on the ideXlab platform.

  • cells within the nodal region of carnation Shoots exhibit a high potential for adventitious Shoot formation
    Plant Cell Tissue and Organ Culture, 1995
    Co-Authors: Anne Claire Van Altvorst, Svetla Yancheva, H J M Dons
    Abstract:

    Adventitious Shoot formation was studied with leaf, stem and axillary bud explants of carnation (Dianthus caryophyllus L.). The Shoot regeneration procedures were applicable for a wide range of cultivars and Shoot regeneration percentages were high for all explant types. Using axillary bud explants, Shoot regeneration efficiency was independent of the size of the bud and of its original position in the plant. In contrast, Shoot regeneration from stem and leaf explants was strongly dependent on their original position on the plant. The most distal explants (just below the apex) showed the highest level of Shoot regeneration. The adventitious Shoot Primordia developed at the periphery of the stem segment and at the base of leaf explants. In axillary bud, stem and leaf explants, Shoot regeneration originated from node cells, located at the transition area between leaf and stem tissue. Moreover, a gradient in Shoot regeneration response was observed, increasing towards the apical meristem.