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Mustafa Yildiz - One of the best experts on this subject based on the ideXlab platform.
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the effect of a submersion pretreatment on in vitro explant growth and Shoot Regeneration from hypocotyls of flax linum usitatissimum
Plant Cell Tissue and Organ Culture, 2004Co-Authors: Mustafa Yildiz, Murat ÖzgenAbstract:This study was carried out to determine the effect of temporary submersion of hypocotyl segments in water on in vitro explant growth and Shoot Regeneration on MS (Murashige and Skoog, 1962) medium supplemented with 1 mg l−1 BAP (6-benzylaminopurine) and 0.02 mg l−1 NAA (naphthaleneacetic acid) in three flax cultivars. It was observed that water-treated hypocotyl explants gave rise to the highest values with respect to Shoot Regeneration percentage, Shoot number per hypocotyl, Shoot length and total Shoot number per Petri dish, successful rooting and plantlet establishment. This procedure may be applicable for other species cultured in vitro.
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High frequency adventitious Shoot Regeneration in sainfoin
Plant Cell Tissue and Organ Culture, 1998Co-Authors: Murat Özgen, Sebahattin Özcan, Cafer S. Sevimay, Cengiz Sancak, Mustafa YildizAbstract:A procedure is described for the rapid and efficient adventitious Shoot Regeneration from leaflets, petioles and stems of field-grown sainfoin plants. All explants formed Shoots on a range of media supplemented with 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). Stem explants appeared to have better Regeneration capacity than leaflet and petiole explants in most media tested. The highest frequency of Shoot Regeneration was achieved from stem segments on a medium containing 20 μM BA and 0.5 μM NAA. Regenerated Shoots rooted in half-strength Murashige and Skoog medium containing 5 μM indole-3-butyric acid and later established well under greenhouse conditions.
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prolific Shoot Regeneration from immature embryo explants of sainfoin onobrychis viciifolia scop
Plant Cell Reports, 1996Co-Authors: Sebahattin Özcan, Cafer S. Sevimay, Cengiz Sancak, Mustafa Yildiz, Murat ÖzgenAbstract:Immature cotyledons and embryo axes of sainfoin were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 6-benzylaminopurine (BAP) and a-naphthaleneacetic acid (NAA) to induce adventitious Shoot Regeneration. The highest frequency of Shoot Regeneration occurred following an initial callus growth on a MS medium containing 0.5 mg/l BAP and 2 mg/l NAA. Immature embryo axes showed higher Regeneration capacity than immature cotyledons, however, Shoot elongation was best achieved on immature cotyledons. Regenerated Shoots were excised and rooted in half strength MS medium with 1 mg/l indole-butyric acid (IBA) or 1 mg/l NAA. The rooted plantlets were finally transferred to compost.
Sebahattin Özcan - One of the best experts on this subject based on the ideXlab platform.
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Adventitious Shoot Regeneration and micropropagation in Calendula officinalis L.
Biologia plantarum, 2004Co-Authors: Sati Cocu, S Uranbey, Arif Ipek, Khalid Mahmood Khawar, Ercüment Osman Sarihan, Mehmet Demir Kaya, Iskender Parmaksiz, Sebahattin ÖzcanAbstract:Hypocotyl, cotyledon and cotyledonary node explants of Calendula officinalis L were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of thidiazuron (TDZ), kinetin (KIN), α-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) to induce adventitious Shoot Regeneration and micropropagation. The highest frequency of adventitious Shoot Regeneration was achieved from hypocotyl and cotyledon explants on MS media supplemented with 0.75 mg dm−3 TDZ and either 0.25 or 0.50 mg dm−3 IBA. Efficient in vitro clonal propagation was also induced from cotyledonary nodes on a range of media supplemented with 0.75 mg dm−3 TDZ and 0.05 mg dm−3 NAA or 2 mg dm−3 KIN and 1 mg dm−3 NAA. Regenerated Shoots were excised and rooted in MS medium supplemented with 1 mg dm−3 NAA. The rooted plantlets were finally transferred to pots.
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High frequency adventitious Shoot Regeneration in sainfoin
Plant Cell Tissue and Organ Culture, 1998Co-Authors: Murat Özgen, Sebahattin Özcan, Cafer S. Sevimay, Cengiz Sancak, Mustafa YildizAbstract:A procedure is described for the rapid and efficient adventitious Shoot Regeneration from leaflets, petioles and stems of field-grown sainfoin plants. All explants formed Shoots on a range of media supplemented with 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). Stem explants appeared to have better Regeneration capacity than leaflet and petiole explants in most media tested. The highest frequency of Shoot Regeneration was achieved from stem segments on a medium containing 20 μM BA and 0.5 μM NAA. Regenerated Shoots rooted in half-strength Murashige and Skoog medium containing 5 μM indole-3-butyric acid and later established well under greenhouse conditions.
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prolific Shoot Regeneration from immature embryo explants of sainfoin onobrychis viciifolia scop
Plant Cell Reports, 1996Co-Authors: Sebahattin Özcan, Cafer S. Sevimay, Cengiz Sancak, Mustafa Yildiz, Murat ÖzgenAbstract:Immature cotyledons and embryo axes of sainfoin were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 6-benzylaminopurine (BAP) and a-naphthaleneacetic acid (NAA) to induce adventitious Shoot Regeneration. The highest frequency of Shoot Regeneration occurred following an initial callus growth on a MS medium containing 0.5 mg/l BAP and 2 mg/l NAA. Immature embryo axes showed higher Regeneration capacity than immature cotyledons, however, Shoot elongation was best achieved on immature cotyledons. Regenerated Shoots were excised and rooted in half strength MS medium with 1 mg/l indole-butyric acid (IBA) or 1 mg/l NAA. The rooted plantlets were finally transferred to compost.
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high frequency adventitious Shoot Regeneration from immature cotyledons of pea pisum sativum l
Plant Cell Reports, 1992Co-Authors: Sebahattin Özcan, Mehdi Barghchi, Simon Firek, John DraperAbstract:A procedure has been developed which allows high frequency adventitious Shoot Regeneration from immature cotyledons of pea. Prolific Shoot Regeneration occurred following an initial callus growth on a Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 4 mg/l α-naphthaleneacetic acid (NAA). Cotyledon expiants proximal to the embryonic axis had the highest Regeneration potential, however, the presence of an embryonic axis inhibited adventitious Shoot Regeneration. Addition of silver nitrate (AgNO3) to the medium did not promote the number of regenerated Shoots but resulted in Shoots with well developed tendrils and large stipules which had a reduced rooting capacity. Regenerated Shoots rooted readily (80-90%) in half strength MS medium containing 1 mg/l indole-butyric acid (IBA) and further established well in compost.
Murat Özgen - One of the best experts on this subject based on the ideXlab platform.
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the effect of a submersion pretreatment on in vitro explant growth and Shoot Regeneration from hypocotyls of flax linum usitatissimum
Plant Cell Tissue and Organ Culture, 2004Co-Authors: Mustafa Yildiz, Murat ÖzgenAbstract:This study was carried out to determine the effect of temporary submersion of hypocotyl segments in water on in vitro explant growth and Shoot Regeneration on MS (Murashige and Skoog, 1962) medium supplemented with 1 mg l−1 BAP (6-benzylaminopurine) and 0.02 mg l−1 NAA (naphthaleneacetic acid) in three flax cultivars. It was observed that water-treated hypocotyl explants gave rise to the highest values with respect to Shoot Regeneration percentage, Shoot number per hypocotyl, Shoot length and total Shoot number per Petri dish, successful rooting and plantlet establishment. This procedure may be applicable for other species cultured in vitro.
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High frequency adventitious Shoot Regeneration in sainfoin
Plant Cell Tissue and Organ Culture, 1998Co-Authors: Murat Özgen, Sebahattin Özcan, Cafer S. Sevimay, Cengiz Sancak, Mustafa YildizAbstract:A procedure is described for the rapid and efficient adventitious Shoot Regeneration from leaflets, petioles and stems of field-grown sainfoin plants. All explants formed Shoots on a range of media supplemented with 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). Stem explants appeared to have better Regeneration capacity than leaflet and petiole explants in most media tested. The highest frequency of Shoot Regeneration was achieved from stem segments on a medium containing 20 μM BA and 0.5 μM NAA. Regenerated Shoots rooted in half-strength Murashige and Skoog medium containing 5 μM indole-3-butyric acid and later established well under greenhouse conditions.
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prolific Shoot Regeneration from immature embryo explants of sainfoin onobrychis viciifolia scop
Plant Cell Reports, 1996Co-Authors: Sebahattin Özcan, Cafer S. Sevimay, Cengiz Sancak, Mustafa Yildiz, Murat ÖzgenAbstract:Immature cotyledons and embryo axes of sainfoin were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 6-benzylaminopurine (BAP) and a-naphthaleneacetic acid (NAA) to induce adventitious Shoot Regeneration. The highest frequency of Shoot Regeneration occurred following an initial callus growth on a MS medium containing 0.5 mg/l BAP and 2 mg/l NAA. Immature embryo axes showed higher Regeneration capacity than immature cotyledons, however, Shoot elongation was best achieved on immature cotyledons. Regenerated Shoots were excised and rooted in half strength MS medium with 1 mg/l indole-butyric acid (IBA) or 1 mg/l NAA. The rooted plantlets were finally transferred to compost.
Kevin M Crosby - One of the best experts on this subject based on the ideXlab platform.
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Shoot Regeneration and ploidy variation in tissue culture of honeydew melon (Cucumis melo L. inodorus)
In Vitro Cellular & Developmental Biology - Plant, 2013Co-Authors: Yan Ren, Haejeen Bang, Bhimanagouda S Patil, Jean Gould, Keerti S. Rathore, Kevin M CrosbyAbstract:The Cucumis melo L. inodorus honeydew melon variant is one of the most consumed melons in the USA, and has important commercial and nutritional value. There is a need for improvement of several genetic traits in the US honeydew melon, such as nutrition, drought tolerance, and disease resistance. We investigated the existing Regeneration media and optimized the medium composition for an elite honeydew diploid breeding line, “150”, using cotyledonary explants. Four combinations of three different plant growth regulators, 6-benzyladenine, abscisic acid, and indole-3-acetic acid (IAA), were tested in the Shoot Regeneration media. The presence of IAA in the medium caused the cotyledon explants to curl away from the medium, which made antibiotic selection problematic in our previous study. Omission of IAA from the culture media eliminated this problem and did not impact the Shoot Regeneration capacity of the cotyledonary explants. We also estimated the ploidy of regenerated plants using flow cytometry, and 50–60% were found to be polyploid (tetraploid or mixoploid). However, contrary to other studies, these polyploid plants did not show major morphological differences compared to the diploid plants.
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agrobacterium mediated transformation and Shoot Regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons cucumis melo l
Plant Cell Tissue and Organ Culture, 2012Co-Authors: Yan Ren, Haejeen Bang, Ian S Curtis, Jean H Gould, Bhimanagouda S Patil, Kevin M CrosbyAbstract:A reliable Agrobacterium-mediated transformation and Shoot Regeneration protocol was developed for breeding lines of commercially important western-shipper cantaloupe and honeydew melons, ‘F39’ and ‘150’, respectively. Different media were tested to select a Shoot Regeneration system for each of three elite breeding lines ‘F39’, ‘141’ and ‘TMS’. Murashige & Skoog (MS) basal medium supplemented with 1 mg l−1 benzyladenine (BA), 0.26 mg l−1 abscisic acid (ABA) and 0.8 mg l−1 indole-3-acetic acid (IAA) was used for Shoot Regeneration from cotyledonary explants in ‘F39’ and ‘150’. Kanamycin sensitivity as well as Timentin™ and Clavamox® were evaluated using wild-type ‘F39’ and ‘150’ cotyledons. Kanamycin concentrations of 200 and 150 mg l−1 were chosen as the threshold levels for ‘F39’ and ‘150’, respectively. No significant differences were found between Timentin™ and Clavamox® in ‘F39’; however, Clavamox® reduced the incidence of vitrification and increased the frequency of Shoot elongation in ‘150’. A. tumefaciens strain EHA105, harboring pCNL56 carrying neomycin phosphotransferase II (nptII) and gusA reporter genes, was selected to establish a transformation protocol for ‘F39’ and ‘150’. Putative transformants were evaluated using β-glucuronidase (GUS) histochemical assay, polymerase chain reaction (PCR) and Southern blot analyses. Based on these parameters, the transformation efficiency for cantaloupe ‘F39’ was 0.3% and that for honeydew ‘150’ was 0.5%.
Xiangyang Kang - One of the best experts on this subject based on the ideXlab platform.
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adventitious Shoot Regeneration from leaf petiole and root explants in triploid populus alba p glandulosa p tomentosa
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Qingqing Zeng, Xiangyang KangAbstract:Leaf, petiole and root of triploid (Populus alba × P. glandulosa) × P. tomentosa were cultured on a medium containing benzyladenine (BA) and naphthaleneacetic acid (NAA) either alone or in combination with thidiazuron (TDZ) for the study of adventitious Shoot Regeneration. The study demonstrated that TDZ played a primary and distinctive role in Shoot Regeneration: the frequency of Shoot organogenesis of explants cultured with TDZ (71.16%) far surpassed those cultured without TDZ (30.42%). Among explant types, high variation was observed in the Regeneration capacity: petiole and leaf had better Regeneration capacity than that of root. To understand this difference, an analysis of endogenous hormones (zeatin (ZT), abscisic acid (ABA) and indole-3-acetic acid (IAA)) was conducted on leaf, petiole and root explants. The results showed that the Regeneration ability of explants varied dependently on their IAA and ABA contents. The organogenic capacity varied significantly with the position of the explant along the Shoot axis. The highest Regeneration potential (98.33%) and Shoot number (up to 9.4 Shoots/explant) were obtained in leaf explants harvested from the intermediate node (node 3). Regenerated Shoots transferred to the medium containing 0.5 mg l−1 indole-3-butyric acid (IBA) achieved the highest frequency of rooting (100%), with means of 5.33 roots per Shoot and 6.32 cm length per root. The Shoots with an abundant root system were later transferred to the greenhouse for further growth and development. The effect of plant growth regulators (PGRs), explant type, endogenous hormone contents and maturity of explant was evaluated on adventitious Shoot Regeneration of triploid (Populus alba × P. glandulosa) × P. tomentosa in this study.
