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Moor Plantation - One of the best experts on this subject based on the ideXlab platform.
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Effect of phytohormones and genotype on meristem and Shoot Tip Culture of Telfairia occidentalis Hook F.
2012Co-Authors: Moor PlantationAbstract:Objectives: The aim of this work was to investigate the effect of plant growth regulators (PGR), genotype and explant type on in vitro Shoot induction, elongation, and mulTiplication in Telfairia occidentalis . Methodology and Results : In this study attempts were made to induce Shoots from meristem and Shoot Tip Culture of Telfairia occidentalis Hook F . Shoot apical meristems with 1-2 leaf primordia and Shoot Tip explants were aseptically isolated and Cultured on ½ N6 medium supplemented with different combinations of 2mg/l BAP, IAA and kinetin. Explants were derived from seedlings of two T. occidentalis genotypes grown in sterilized soils inside vials in the laboratory. Regeneration response were examined based on five parameters – callus formation, Shoot length, number of Shoots per explant, number of leaves per explant and number of nodes. Shoot induction, elongation and mulTiplication were most effectively promoted by the medium supplemented with 2.0 mg/lBAP + 2.0 mg/l IAA for Shoot Tip Culture, while 2.0 mg/l BAP was most effective for Shoot regeneration from meristem Culture. Genotype I was significantly higher than Genotype II in Shoot bud regeneration response (p
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effect of phytohormones and genotype on meristem and Shoot Tip Culture of telfairia occidentalis hook f
2012Co-Authors: Moor PlantationAbstract:Objectives: The aim of this work was to investigate the effect of plant growth regulators (PGR), genotype and explant type on in vitro Shoot induction, elongation, and mulTiplication in Telfairia occidentalis . Methodology and Results : In this study attempts were made to induce Shoots from meristem and Shoot Tip Culture of Telfairia occidentalis Hook F . Shoot apical meristems with 1-2 leaf primordia and Shoot Tip explants were aseptically isolated and Cultured on ½ N6 medium supplemented with different combinations of 2mg/l BAP, IAA and kinetin. Explants were derived from seedlings of two T. occidentalis genotypes grown in sterilized soils inside vials in the laboratory. Regeneration response were examined based on five parameters – callus formation, Shoot length, number of Shoots per explant, number of leaves per explant and number of nodes. Shoot induction, elongation and mulTiplication were most effectively promoted by the medium supplemented with 2.0 mg/lBAP + 2.0 mg/l IAA for Shoot Tip Culture, while 2.0 mg/l BAP was most effective for Shoot regeneration from meristem Culture. Genotype I was significantly higher than Genotype II in Shoot bud regeneration response (p<0.05). Culture of explants from Shoot Tip led to better Shoot regeneration in comparison to explants from meristem. Callus formation/induction was also influenced by explants and media interaction. Conclusion and Application of Findings: In vitro Shoot induction from meristem and Shoot Tip Culture of T . occidentalis has been demonstrated. These in vitro Culture procedures would be useful for developing uniform clones or micropropagation and could also form the basis for in vitro storage of explants and subsequent regeneration of plantlets after long term conservation in this species. The recalcitrant nature its seeds makes alternative means of genetic resources conservation very necessary. More importantly, meristem Culture technique is useful for developing virus-free clones and avoids the limitations imposed by conventional mode of planting. Calli produced could also be excellent targets for genetic transformation and
Genene Gezahegn - One of the best experts on this subject based on the ideXlab platform.
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In vitro regeneration of disease free enset [Ensete ventricosum (Welw) Cheesman] planting materials from bacterial wilt diseased plants using Shoot Tip Culture
African Journal of Biotechnology, 2016Co-Authors: Genene Gezahegn, Firew MekbibAbstract:Enset is an important food crop produced in Ethiopia with great role in food security. The demand of the crop is increasing throughout the country. However, the production as a whole is decreasing due to devastation by enset bacterial wilt. The studies were conducted to develop the procedure for obtaining mulTiple disease free plantlets from infected enset plants. Shoot Tip explants from infected suckers of three clones Arkiya, Digomerza and Mazia were Cultured on MS media supplemented with different combinations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) each with concentration of 0, 1.5, 3, 4.5 and 6 mg/L with 0, 0.5, 1, 1.5 and 2 mg/L, respectively. The effect of growth regulators on Shoot growth parameters was examined. The minimum days (11.66) for mulTiple Shoot induction were recorded for Mazia on media with 4.5 mg/L BAP and 1.5 m/L NAA. The maximum number of Shoots (23.0) was also obtained for Mazia on the same hormone combination as for days of induction. Whereas, the maximum Shoot length (8.1 cm) was recorded for Digomerza on media with 3 and 1 mg/L NAA. Similarly, for root induction and growth MS media with different concentrations of indulebutyric acid (IBA; 0, 0.5, 1, 1.5 and 2 mg/L) were evaluated. The minimum days (10.5) to root induction was observed for Mazia on media with 1.5 mg/L IBA and the maximum root number (3.8) was recorded at 2 mg/L IBA. In the efficiency of Shoot Tip Culture for Xanthomonas pathogen elimination, sample suspension was prepared from Shoots regenerated from diseased suckers and transferred on semi-selective yeast peptone sucrose agar (YPSA) medium. The result of colony observation indicated that many microbes are living in enset saprophytically as mixed colony growth was observed within 24 h after sample culturing. Pathogenesity test on clean suckers of susceptible clone showed that the colonies grown were due to endophytic microbes since none of the colonies were capable to develop disease symptoms as sample of the pathogen strain. Key words: Ensete ventricosum, benzylaminopurine (BAP), indulebutyric acid (IBA), naphthaleneacetic acid (NAA), Shoot Tip, Xanthomonas, yeast peptone sucrose agar (YPSA).
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In vitro Regeneration of Disease Free Enset (Ensete ventricosum (Welw) Cheesman) Planting Materials from Bacterial Wilt Diseased Plants Using Shoot Tip Culture
Journal of Biology Agriculture and Healthcare, 2016Co-Authors: Genene GezahegnAbstract:Enset is an important food crop produced in Ethiopia with great role in food security. The demand of the crop is increasing throughout the country. However, the production as the whole is decreasing due to devastation by enset bacterial wilt. The studies were conducted to develop the procedure for obtaining mulTiple disease free plantlets from infected enset plants. Shoot Tip explants from infected suckers of three clones Arkiya, Digomerza and Mazia were Cultured on MS media supplemented with different combinations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) each with concentration of 0, 1.5, 3, 4.5 and 6 mg/L with 0, 0.5, 1, 1.5 and 2 mg/L, respectively. The effect of growth regulators on Shoot growth parameters was examined. The minimum days (11.66) for mulTiple Shoot induction were recorded for Mazia on media with 4.5 mg/L BAP and 1.5 m/L NAA. The maximum number of Shoots (23.0) was also obtained for Mazia on the same hormone combination as for days of induction. Whereas, the maximum Shoot length (8.1 cm) was recorded for Digomerza on media with 3 and 1 mg/L NAA. Similarly, for root induction and growth MS media with different concentrations of indulebutyric acid (IBA; 0, 0.5, 1, 1.5 and 2 mg/L) were evaluated. The minimum days (10.5) to root induction was observed for Mazia on media with 1.5 mg/L IBA and the maximum root number (3.8) was recorded at 2 mg/L IBA. In the efficiency of Shoot Tip Culture for Xanthomonas pathogen elimination, sample suspension was prepared from Shoots regenerated from diseased suckers and transferred on semi-selective yeast peptone sucrose agar (YPSA) medium. The result of colony observation indicated that many microbes are living in enset saprophytically as mixed colony growth was observed within 24 h after sample culturing. Pathogenesity test on clean suckers of susceptible clone showed that the colonies grown were due to endophytic microbes since none of the colonies were capable to develop disease symptoms as sample of the pathogen strain. Keywords: Ensete ventricosum, benzylaminopurine (BAP), indulebutyric acid (IBA), naphthaleneacetic acid (NAA), Shoot Tip, Xanthomonas, yeast peptone sucrose agar (YPSA).
Xiansong Yang - One of the best experts on this subject based on the ideXlab platform.
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RAPID PRODUCTION OF VIRUS-FREE PLANTLETS BY Shoot Tip Culture IN VITRO OF PURPLE-COLOURED SWEET POTATO (IPOMOEA BATATAS (L.) LAM.)
Pakistan Journal of Botany, 2010Co-Authors: Xiansong YangAbstract:A rapid virus-free seedlings formation protocol was established for purple-coloured sweet potato by Shoot Tip Culture In vitro. The effects of two factors, namely BAP, NAA and their interaction, on callus, roots, buds and rooted plantlets initiation were evaluated by orthogonal design with two factors and four levels. The variance analysis of the experimental results showed that the actions of the two factors and their interaction had significantly different effects on callus, bud, root and rooted plantlet initiation. The best medium for adventitious bud induction was the combination of solid MS supplemented with 1.0 mg l −1 BAP. However, the best medium for rooted plantlet was the combination of solid MS supplemented with 0.5 mg l −1 BAP and 0.2 mg l −1 NAA. Rooted plantlets were acclimatized to greenhouse conditions and appeared normal. The plantlets from Shoot Tip tissue Culture were transplanted successfully. At the same time, the regenerated seedlings were surveyed by the method of indicator plant and enzyme-linked immunosorbent assay on nitrocellulose membranes (NCM-ELISA), and the virus-free plantlets of purple-coloured sweet potato was obtained. In vitro Shoot Tip Culture can be a useful tool in the provision and conservation of virus-free plantlets of purple-coloured sweet potato.
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Studies on Shoot-Tip Culture and virus elimination of the major excellent cultivars of sweet potato in Chongqing
Journal of Southwest China Normal University, 2005Co-Authors: Shuyan Deng, Yongwen Chen, Xiansong Yang, Feng Gao, Qitang ZhangAbstract:In this paper, Shoot-Tip Culture in vitro for eliminating virus was studied by using the excellent sweet potato cultivars which widely spread in Chongqing as the test materials. The results showed that the frequency of plant regeneration of the stem apex which sprouted directly from tuber was the highest, and reached 53. 2%; continuous Shoot-Tip Culture twice can apparently improve the effects of virus elimination; the method of reverse transcription PCR (RT-PCR) is more sensitive and reliable than that of enzyme-linked immunosorbent assay on nitrocellulose membranes (NCM-ELISA).
Nikolaos I. Katis - One of the best experts on this subject based on the ideXlab platform.
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Elimination of a new ampelovirus (GLRaV-Pr) and Grapevine rupestris stem pitting associated virus (GRSPaV) from two Vitis vinifera cultivars combining in vitro thermotherapy with Shoot Tip Culture
Scientia Horticulturae, 2009Co-Authors: Varvara I. Maliogka, F.g. Skiada, Eleftherios P. Eleftheriou, Nikolaos I. KatisAbstract:A new virus species designated as Grapevine leafroll associated virus-Pr (GLRaV-Pr), which is classified in a distinct phylogenetic group of the genus Ampelovirus (Closteroviridae), was recently characterized from Greek grapevine cultivars. Elimination studies of GLRaV-Pr were carried out in two grapevine cultivars, ‘Mantilaria’ and ‘Prevezaniko’, co-infected with Grapevine rupestris stem pitting associated virus (GRSPaV, Flexiviridae). Both viruses were detected by nested RT-PCR assays. Virus elimination was achieved by combining in vitro thermotherapy with meristem (0.2 mm) or Shoot Tip Culture (0.5 cm). The survival and regeneration rate of meristems was very low. On the other hand, high survival rates were observed in the Cultured Shoot Tips accompanied with high elimination rates for both viruses. Data obtained in this study indicate that virus elimination depends on the genotype of grapevine. The results confirmed that sanitation is easier for species of the Closteroviridae family than for GRSPaV, whereas it seems that eradication of GLRaV-Pr and GRSPaV is feasible even with larger plant tissue parts if combined with an appropriate thermotherapy profile in vitro.
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Elimination of Plum pox virus through in vitro thermotherapy and Shoot Tip Culture compared to conventional heat treatment in apricot cultivar Bebecou
Journal of General Plant Pathology, 2007Co-Authors: Georgios C. Koubouris, Varvara I. Maliogka, Nikolaos I. Katis, Kostadinos Efthimiou, Miltiadis VasilakakisAbstract:The effectiveness of in vitro thermotherapy (explants at 35–37°C for 20 days) in obtaining propagating material of apricot (Prunus armeniaca L.) cultivar Bebecou that is free of Plum pox virus (PPV) was compared to a conventional heat treatment technique (potted plants at 30–35°C for 8 weeks). An improved protocol for in vitro thermotherapy was combined with Shoot Tip Culture. The protocol is 5.8 times more effective than conventional thermotherapy while taking half the time and is likely to have a high commercial impact for nurseries.
Gizachew Haile - One of the best experts on this subject based on the ideXlab platform.
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Protocol optimization for in vitro propagation of Kulfo, orange flesh sweet potato (Ipomoea batatas) variety using Shoot Tip Culture
African Journal of Plant Science, 2020Co-Authors: Belachew Beyene, Temesgen Menamo, Gizachew HaileAbstract:Conventional propagation methods of sweet potato (Ipomoea batatas L. var Kulfo) through stem cutting require large amount of propagules and large space for preparation. It has high risk of disease transmission to the next generation. In vitro propagation is the best alternative to overcome such limitations. This study was conducted to optimize protocol for in vitro propagation of Kulfo sweet potato variety using 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) phytohormones for Shoot mulTiplication and rooting, respectively. The result revealed that the highest Shoot initiation (77.78%) and Shoot length (4.40 cm) was observed in MS media supplemented with 0.5 mg/l BAP. Best Shoot mulTiplication (5.33 Shoots per explants) was obtained in MS medium supplemented with 1 mg/l BAP. MS medium supplemented with 0.5 mg/l IBA showed 100% rooting and average root length of 7.44 cm in vitro. In ex vitro conditions, 93.33% rooting was recorded. During acclimatization, 84 and 93% survival of in vitro and ex vitro rooted plantlets, respectively, were recorded. It could be concluded that MS media without BAP provides optimal condition for Shoot initiation. MS supplemented with 1 mg/l BAP provides optimum condition for Shoot mulTiplication. The ex vitro rooting could be better option to reduce in vitro rooting cost and for increased plantlet survival during acclimatization. Based on the result, 0.5 mg/l BAP for Shoot initiation, 1 mg/l BAP of Shoot mulTiplication and directly transferring to soil for rooting was recommended for micropropagation of Kulfo sweet potato variety. However, further studies will be needed in ex vitro root induction. Key words: Sweet potato, micropropagation, Shoot Tip Culture.