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  • Complete mitochondrial genome of a wild Siberian Tiger
    Mitochondrial DNA, 2014
    Co-Authors: Yujiao Sun, Zhaohui Sun, Weijun Guan, Zhensheng Liu, Liwei Teng, Shuo Wang
    Abstract:

    AbstractIn this study, the complete mitochondrial genome of Siberian Tiger (Panthera tigris altaica) was sequenced, using muscle tissue obtained from a male wild Tiger. The total length of the mitochondrial genome is 16,996 bp. The genome structure of this Tiger is in accordance with other Siberian Tigers and it contains 12S rRNA gene, 16S rRNA gene, 22 tRNA genes, 13 protein-coding genes, and 1 control region.

  • Generation and analysis of a large-scale expressed sequence tags from a full-length enriched cDNA library of Siberian Tiger (Panthera tigris altaica)
    Gene, 2014
    Co-Authors: Yu Guo, Changqing Liu, Dan Liu, Chunyu Bai, Weijun Guan
    Abstract:

    Abstract In this study, a full-length enriched cDNA library was successfully constructed from Siberian Tiger, the world's most endangered species. The titers of primary and amplified libraries were 1.28 × 10 6  pfu/mL and 1.59 × 10 10  pfu/mL respectively. The proportion of recombinants from unamplified library was 91.3% and the average length of exogenous inserts was 1.06 kb. A total of 279 individual ESTs with sizes ranging from 316 to 1258 bps were then analyzed. Furthermore, 204 unigenes were successfully annotated and involved in 49 functions of the GO classification, cell (175, 85.5%), cellular process (165, 80.9%), and binding (152, 74.5%) are the dominant terms. 198 unigenes were assigned to 156 KEGG pathways, and the pathways with the most representation are metabolic pathways (18, 9.1%). The proportion pattern of each COG subcategory was similar among Panthera tigris altaica , P. tigris tigris and Homo sapiens , and general function prediction only cluster (44, 15.8%) represents the largest group, followed by translation, ribosomal structure and biogenesis (33, 11.8%), replication, recombination and repair (24, 8.6%), and only 7.2% ESTs classified as novel genes. Moreover, the recombinant plasmid pET32a-TAT-COL6A2 was constructed, coded for the Trx-TAT-COL6A2 fusion protein with two 6 × His-tags in N and C-terminal. After BCA assay, the concentration of soluble Trx-TAT-COL6A2 recombinant protein was 2.64 ± 0.18 mg/mL. This library will provide a useful platform for the functional genome and transcriptome research of for the P. tigris and other felid animals in the future.

  • Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage
    International journal of molecular sciences, 2014
    Co-Authors: Changqing Liu, Dan Liu, Yu Guo, Chunyu Bai, Weijun Guan
    Abstract:

    Bacterial artificial chromosome (BAC) libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian Tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian Tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86% statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library with 19 microsatellite markers and a SRY sequence revealed that each of these markers were present in the library; the average number of positive clones per marker was 6.74 (range 2 to 12), consistent with 6.46 coverage of the Tiger genome. Additionally, we identified 72 microsatellite markers that could potentially be used as genetic markers. This BAC library will serve as a valuable resource for physical mapping, comparative genomic study and large-scale genome sequencing in the Tiger.

  • Article Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage
    2014
    Co-Authors: Changqing Liu, Dan Liu, Yu Guo, Chunyu Bai, Weijun Guan
    Abstract:

    Abstract: Bacterial artificial chromosome (BAC) libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian Tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian Tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86 % statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library wit

  • Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian Tiger
    International journal of biological sciences, 2010
    Co-Authors: Changqing Liu, Dan Liu, Baogang Feng, Weijun Guan
    Abstract:

    In this study we successfully constructed a full-length cDNA library from Siberian Tiger, Panthera tigris altaica, the most well-known wild Animal. Total RNA was extracted from cultured Siberian Tiger fibroblasts in vitro. The titers of primary and amplified libraries were 1.30×106 pfu/ml and 1.62×109 pfu/ml respectively. The proportion of recombinants from unamplified library was 90.5% and average length of exogenous inserts was 1.13 kb. A total of 282 individual ESTs with sizes ranging from 328 to 1,142bps were then analyzed the BLASTX score revealed that 53.9% of the sequences were classified as strong match, 38.6% as nominal and 7.4% as weak match. 28.0% of them were found to be related to enzyme/catalytic protein, 20.9% ESTs to metabolism, 13.1% ESTs to transport, 12.1% ESTs to signal transducer/cell communication, 9.9% ESTs to structure protein, 3.9% ESTs to immunity protein/defense metabolism, 3.2% ESTs to cell cycle, and 8.9 ESTs classified as novel genes. These results demonstrated that the reliability and representativeness of the cDNA library attained to the requirements of a standard cDNA library. This library provided a useful platform for the functional genomic research of Siberian Tigers.

Christoph Schwitzer - One of the best experts on this subject based on the ideXlab platform.

Dan Liu - One of the best experts on this subject based on the ideXlab platform.

  • Haematological and Biochemical Parameters of Captive Siberian Tigers (Panthera tigris altaica) from the Heilongjiang Province, China.
    Veterinary medicine and science, 2020
    Co-Authors: Enqi Liu, Dan Liu, Dan You, Cen Yang, Yajun Wang
    Abstract:

    Haematological and biochemical parameters play important roles in safeguarding animal health and preventing disease, but the blood reference values of many wild animals are still unknown. Recently, few descriptions of the blood parameters of Siberian Tigers (Panthera tigris altaica) have been reported because these Tigers comprise an endangered species; however, it is extremely difficult to obtain blood samples necessary for these analyses. This study presents 14 haematological and 16 biochemical parameters of 133 Siberian Tigers, of which 112 and 21 were from Heilongjiang Siberian Tiger Park (HB) and Hailin Siberian Tiger Park (HD), China, respectively. Our study is the first to determine the following parameters in Siberian Tigers: red blood cell volume distribution width, platelet count, mean platelet volume, amylase (AMY), sodium/potassium, globulin and albumin/globulin levels. As the data for total bilirubin and AMY were not statistically significant, no statistical analysis was conducted for these parameters. Few parameters were significantly different according to sex and region (p 

  • De novo transcriptomic analysis and development of EST-SSR markers in the Siberian Tiger (Panthera tigris altaica)
    Molecular genetics and genomics : MGG, 2016
    Co-Authors: Yujiao Sun, Minghao Zhu, Dan Liu, Hongyan Chen, Guan Weijun
    Abstract:

    The Siberian Tiger, Panthera tigris altaica, is an endangered species, and much more work is needed to protect this species, which is still vulnerable to extinction. Conservation efforts may be supported by the genetic assessment of wild populations, for which highly specific microsatellite markers are required. However, only a limited amount of genetic sequence data is available for this species. To identify the genes involved in the lung transcriptome and to develop additional simple sequence repeat (SSR) markers for the Siberian Tiger, we used high-throughput RNA-Seq to characterize the Siberian Tiger transcriptome in lung tissue (designated ‘PTA-lung’) and a pooled tissue sample (designated ‘PTA’). Approximately 47.5 % (33,187/69,836) of the lung transcriptome was annotated in four public databases (Nr, Swiss-Prot, KEGG, and COG). The annotated genes formed a potential pool for gene identification in the Tiger. An analysis of the genes differentially expressed in the PTA lung, and PTA samples revealed that the Tiger may have suffered a series of diseases before death. In total, 1062 non-redundant SSRs were identified in the Siberian Tiger transcriptome. Forty-three primer pairs were randomly selected for amplification reactions, and 26 of the 43 pairs were also used to evaluate the levels of genetic polymorphism. Fourteen primer pairs (32.56 %) amplified products that were polymorphic in size in P. tigris altaica. In conclusion, the transcriptome sequences will provide a valuable genomic resource for genetic research, and these new SSR markers comprise a reasonable number of loci for the genetic analysis of wild and captive populations of P. tigris altaica.

  • Generation and analysis of a large-scale expressed sequence tags from a full-length enriched cDNA library of Siberian Tiger (Panthera tigris altaica)
    Gene, 2014
    Co-Authors: Yu Guo, Changqing Liu, Dan Liu, Chunyu Bai, Weijun Guan
    Abstract:

    Abstract In this study, a full-length enriched cDNA library was successfully constructed from Siberian Tiger, the world's most endangered species. The titers of primary and amplified libraries were 1.28 × 10 6  pfu/mL and 1.59 × 10 10  pfu/mL respectively. The proportion of recombinants from unamplified library was 91.3% and the average length of exogenous inserts was 1.06 kb. A total of 279 individual ESTs with sizes ranging from 316 to 1258 bps were then analyzed. Furthermore, 204 unigenes were successfully annotated and involved in 49 functions of the GO classification, cell (175, 85.5%), cellular process (165, 80.9%), and binding (152, 74.5%) are the dominant terms. 198 unigenes were assigned to 156 KEGG pathways, and the pathways with the most representation are metabolic pathways (18, 9.1%). The proportion pattern of each COG subcategory was similar among Panthera tigris altaica , P. tigris tigris and Homo sapiens , and general function prediction only cluster (44, 15.8%) represents the largest group, followed by translation, ribosomal structure and biogenesis (33, 11.8%), replication, recombination and repair (24, 8.6%), and only 7.2% ESTs classified as novel genes. Moreover, the recombinant plasmid pET32a-TAT-COL6A2 was constructed, coded for the Trx-TAT-COL6A2 fusion protein with two 6 × His-tags in N and C-terminal. After BCA assay, the concentration of soluble Trx-TAT-COL6A2 recombinant protein was 2.64 ± 0.18 mg/mL. This library will provide a useful platform for the functional genome and transcriptome research of for the P. tigris and other felid animals in the future.

  • Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage
    International journal of molecular sciences, 2014
    Co-Authors: Changqing Liu, Dan Liu, Yu Guo, Chunyu Bai, Weijun Guan
    Abstract:

    Bacterial artificial chromosome (BAC) libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian Tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian Tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86% statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library with 19 microsatellite markers and a SRY sequence revealed that each of these markers were present in the library; the average number of positive clones per marker was 6.74 (range 2 to 12), consistent with 6.46 coverage of the Tiger genome. Additionally, we identified 72 microsatellite markers that could potentially be used as genetic markers. This BAC library will serve as a valuable resource for physical mapping, comparative genomic study and large-scale genome sequencing in the Tiger.

  • Article Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage
    2014
    Co-Authors: Changqing Liu, Dan Liu, Yu Guo, Chunyu Bai, Weijun Guan
    Abstract:

    Abstract: Bacterial artificial chromosome (BAC) libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian Tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian Tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86 % statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library wit

Changqing Liu - One of the best experts on this subject based on the ideXlab platform.

  • Generation and analysis of a large-scale expressed sequence tags from a full-length enriched cDNA library of Siberian Tiger (Panthera tigris altaica)
    Gene, 2014
    Co-Authors: Yu Guo, Changqing Liu, Dan Liu, Chunyu Bai, Weijun Guan
    Abstract:

    Abstract In this study, a full-length enriched cDNA library was successfully constructed from Siberian Tiger, the world's most endangered species. The titers of primary and amplified libraries were 1.28 × 10 6  pfu/mL and 1.59 × 10 10  pfu/mL respectively. The proportion of recombinants from unamplified library was 91.3% and the average length of exogenous inserts was 1.06 kb. A total of 279 individual ESTs with sizes ranging from 316 to 1258 bps were then analyzed. Furthermore, 204 unigenes were successfully annotated and involved in 49 functions of the GO classification, cell (175, 85.5%), cellular process (165, 80.9%), and binding (152, 74.5%) are the dominant terms. 198 unigenes were assigned to 156 KEGG pathways, and the pathways with the most representation are metabolic pathways (18, 9.1%). The proportion pattern of each COG subcategory was similar among Panthera tigris altaica , P. tigris tigris and Homo sapiens , and general function prediction only cluster (44, 15.8%) represents the largest group, followed by translation, ribosomal structure and biogenesis (33, 11.8%), replication, recombination and repair (24, 8.6%), and only 7.2% ESTs classified as novel genes. Moreover, the recombinant plasmid pET32a-TAT-COL6A2 was constructed, coded for the Trx-TAT-COL6A2 fusion protein with two 6 × His-tags in N and C-terminal. After BCA assay, the concentration of soluble Trx-TAT-COL6A2 recombinant protein was 2.64 ± 0.18 mg/mL. This library will provide a useful platform for the functional genome and transcriptome research of for the P. tigris and other felid animals in the future.

  • Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage
    International journal of molecular sciences, 2014
    Co-Authors: Changqing Liu, Dan Liu, Yu Guo, Chunyu Bai, Weijun Guan
    Abstract:

    Bacterial artificial chromosome (BAC) libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian Tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian Tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86% statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library with 19 microsatellite markers and a SRY sequence revealed that each of these markers were present in the library; the average number of positive clones per marker was 6.74 (range 2 to 12), consistent with 6.46 coverage of the Tiger genome. Additionally, we identified 72 microsatellite markers that could potentially be used as genetic markers. This BAC library will serve as a valuable resource for physical mapping, comparative genomic study and large-scale genome sequencing in the Tiger.

  • Article Construction and Analysis of Siberian Tiger Bacterial Artificial Chromosome Library with Approximately 6.5-Fold Genome Equivalent Coverage
    2014
    Co-Authors: Changqing Liu, Dan Liu, Yu Guo, Chunyu Bai, Weijun Guan
    Abstract:

    Abstract: Bacterial artificial chromosome (BAC) libraries are extremely valuable for the genome-wide genetic dissection of complex organisms. The Siberian Tiger, one of the most well-known wild primitive carnivores in China, is an endangered animal. In order to promote research on its genome, a high-redundancy BAC library of the Siberian Tiger was constructed and characterized. The library is divided into two sub-libraries prepared from blood cells and two sub-libraries prepared from fibroblasts. This BAC library contains 153,600 individually archived clones; for PCR-based screening of the library, BACs were placed into 40 superpools of 10 × 384-deep well microplates. The average insert size of BAC clones was estimated to be 116.5 kb, representing approximately 6.46 genome equivalents of the haploid genome and affording a 98.86 % statistical probability of obtaining at least one clone containing a unique DNA sequence. Screening the library wit

  • Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian Tiger
    International journal of biological sciences, 2010
    Co-Authors: Changqing Liu, Dan Liu, Baogang Feng, Weijun Guan
    Abstract:

    In this study we successfully constructed a full-length cDNA library from Siberian Tiger, Panthera tigris altaica, the most well-known wild Animal. Total RNA was extracted from cultured Siberian Tiger fibroblasts in vitro. The titers of primary and amplified libraries were 1.30×106 pfu/ml and 1.62×109 pfu/ml respectively. The proportion of recombinants from unamplified library was 90.5% and average length of exogenous inserts was 1.13 kb. A total of 282 individual ESTs with sizes ranging from 328 to 1,142bps were then analyzed the BLASTX score revealed that 53.9% of the sequences were classified as strong match, 38.6% as nominal and 7.4% as weak match. 28.0% of them were found to be related to enzyme/catalytic protein, 20.9% ESTs to metabolism, 13.1% ESTs to transport, 12.1% ESTs to signal transducer/cell communication, 9.9% ESTs to structure protein, 3.9% ESTs to immunity protein/defense metabolism, 3.2% ESTs to cell cycle, and 8.9 ESTs classified as novel genes. These results demonstrated that the reliability and representativeness of the cDNA library attained to the requirements of a standard cDNA library. This library provided a useful platform for the functional genomic research of Siberian Tigers.

  • Establishment and Characterization of Fibroblast Cell Line Derived from Siberian Tiger (Panthera tigris altaica).
    Biopreservation and biobanking, 2010
    Co-Authors: Changqing Liu, Dan Liu, Yu Guo, Weijun Guan
    Abstract:

    The Siberian Tiger ear marginal tissue fibroblast cell line (STF34) from 34 samples was successfully established using primary explants technique and cell cryoconservation technology. STF34 cells were adherent, with a population doubling time of 24 h. Chromosome analysis showed that 90.2%–91.6% of cells were diploid (2n = 38). Isoenzyme analyses of lactate dehydrogenase and malate dehydrogenase showed that STF34 cells had no cross-contamination with other species. Tests for cell line contamination with bacteria, fungi, viruses, and mycoplasmas were all negative. Every index of the STF34 cell line meets all the standard quality controls of American Type Culture Collection. Not only has the germline of this important Siberian Tiger species been preserved at the cell level, but also valuable material had been provided for genome, postgenome, and somacloning research.

Akos Somoskovi - One of the best experts on this subject based on the ideXlab platform.

  • pulmonary tuberculosis due to mycobacterium bovis subsp caprae in captive Siberian Tiger
    Emerging Infectious Diseases, 2003
    Co-Authors: Ákos Lantos, Stefan Niemann, Endre Sós, Károly Erdélyi, Sándor Dávid, Linda M. Parsons, Tanja Kubica, László Mezõsi, Sabine Ruschgerdes, Akos Somoskovi
    Abstract:

    We report the first case of pulmonary tuberculosis caused by Mycobacterium bovis subsp. caprae in a captive Siberian Tiger, an endangered feline. The pathogen was isolated from a tracheal aspirate obtained by bronchoscopy. This procedure provided a reliable in vivo diagnostic method in conjunction with conventional and molecular tests for the detection of mycobacteria.

  • pulmonary tuberculosis due to mycobacterium bovis in captive Siberian Tiger
    Emerging Infectious Diseases, 2003
    Co-Authors: Ákos Lantos, Stefan Niemann, László Mezősi, Endre Sós, Károly Erdélyi, Sándor Dávid, Linda M. Parsons, Tanja Kubica, Sabine Ruschgerdes, Akos Somoskovi
    Abstract:

    Mycobacterium bovis, a member of the M. tuberculosis complex (MTBC), can cause tuberculosis in a wide range of domestic and wild animals and also in humans (1,2). Routine differentiation of M. bovis is based on a number of phenotypic characteristics and biochemical tests (2). M. bovis shows dysgonic growth on Lowenstein-Jensen (LJ) medium and has been described as negative for nitrate reduction and niacin accumulation (2). As a further criterion for the differentiation of M. bovis, intrinsic resistance to pyrazinamide (PZA) has been described (2). However, more recently, PZA-susceptible strains of M. bovis were found in Spain and Germany; these strains were also characterized by specific molecular techniques (3–5). As a consequence, M. bovis was split into two subspecies: M. bovis subsp. bovis, which showed resistance to PZA, and M. bovis subsp. caprae, which was sensitive to PZA (6,7). M. bovis subsp. caprae was initially isolated from sheep and goats in Spain (3,4,7); however, further studies confirm its infectivity in humans, cattle, and red deer (6,8). We report the unusual case of a M. bovis subsp. caprae infection in a captive Siberian Tiger.

  • Mycobacterium bovisin Captive Siberian Tiger
    Emerging infectious diseases, 2003
    Co-Authors: Ákos Lantos, Stefan Niemann, László Mezősi, Endre Sós, Károly Erdélyi, Sándor Dávid, Linda M. Parsons, Tanja Kubica, Sabine Rüsch-gerdes, Akos Somoskovi
    Abstract:

    We report the first case of pulmonary tuberculosis caused by Mycobacterium bovis subsp. caprae in a captive Siberian Tiger, an endangered feline. The pathogen was isolated from a tracheal aspirate obtained by bronchoscopy. This procedure provided a reliable in vivo diagnostic method in conjunction with conventional and molecular tests for the detection of mycobacteria.

  • Pulmonary Tuberculosis due to Mycobacterium bovis in Captive Siberian Tiger
    Centers for Disease Control and Prevention, 2003
    Co-Authors: Ákos Lantos, Stefan Niemann, László Mezősi, Endre Sós, Károly Erdélyi, Sándor Dávid, Linda M. Parsons, Tanja Kubica, Sabine Rüsch-gerdes, Akos Somoskovi
    Abstract:

    We report the first case of pulmonary tuberculosis caused by Mycobacterium bovis subsp. caprae in a captive Siberian Tiger, an endangered feline. The pathogen was isolated from a tracheal aspirate obtained by bronchoscopy. This procedure provided a reliable in vivo diagnostic method in conjunction with conventional and molecular tests for the detection of mycobacteria