The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform

Michele Carbone - One of the best experts on this subject based on the ideXlab platform.

  • The relationship between Simian Virus 40 and mesothelioma.
    Current opinion in pulmonary medicine, 2008
    Co-Authors: Zeyana Rivera, Harvey I Pass, Oriana Strianese, Pietro Bertino, Haining Yang, Michele Carbone
    Abstract:

    Purpose of reviewSimian Virus 40 is present in some human malignant mesotheliomas. The evidence in favor and against a pathogenic role of Simian Virus 40 in malignant mesothelioma is discussed in this review.Recent findingsWhen Simian Virus 40 is injected intracardially into hamsters, 60% develop an

  • human mesotheliomas contain the Simian Virus 40 regulatory region and large tumor antigen dna sequences
    The Journal of Thoracic and Cardiovascular Surgery, 1998
    Co-Authors: Jessica S Donington, Harvey I Pass, Paola Rizzo, Michael Nishimura, Ronald C Kennedy, Michele Carbone
    Abstract:

    Abstract Background: A cohort (20%) of patients with mesothelioma will not have an exposure to asbestos. Recently, a DNA tumor Virus (Simian Virus 40) has been shown to cause hamster mesotheliomas; we previously described Simian Virus 40–like DNA amino terminus sequences in 29 of 48 mesotheliomas. We analyzed an additional 42 mesotheliomas to determine (1) whether our initial observations were durable and (2) the extent to which the Simian Virus 40 genome is present in mesotheliomas. Methods: Genomic DNA was extracted from snap frozen mesothelioma tumor samples and from the Simian Virus 40–induced hamster mesothelioma tumor H9A. Polymerase chain reaction primers were used to amplify various Simian Virus 40 large T-antigen regions including a 105–base pair amino terminus fragment, a 281–base pair carboxyl terminus fragment, and a 310–base pair fragment of the enhancer promoter region. Endonuclease digestions and Southern blotting were used to verify the expected product. Results: Thirty of the 42 (71%) samples amplified T-antigen amino sequences, and specificity was verified by Southern hybridization. Sixteen of 42 samples (38%) amplified the appropriate size fragment for the carboxyl terminus, and digestion with BsaB1 matched that of H9A. Twenty-two of 42 samples (52%) amplified Simian Virus 40 regulatory sequences and Fok 1 digestion matched that of the hamster control tumor. Sequence analysis (4 patients) revealed 100% homology with the regulatory region of Simian Virus 40 strain 776. Conclusions: These data suggest an association between the Simian Virus 40 Virus and human mesothelioma that could be exploited for diagnostic/therapeutic options including early detection and potential vaccination strategies. (J Thorac Cardiovasc Surg 1998;116:854-9)

  • Simian Virus 40 and human cancer.
    Monaldi archives for chest disease = Archivio Monaldi per le malattie del torace, 1998
    Co-Authors: Luciano Mutti, Michele Carbone, Gg Giordano, Antonio Giordano
    Abstract:

    Deoxyribonucleic acid (DNA) oncoViruses can induce neoplastic transformation by interfering with proliferative proteins. Simian Virus 40 (SV40) has been shown to induce brain tumors, osteosarcoma, lymphoid tumors and malignant mesothelioma in hamsters and SV40-like DNA sequences corresponding to the Rb-pocket binding domain of SV40 T-antigen (Tag) have been detected in the same human tumors. Since only a small percentage of people exposed to asbestos fibers develop a malignant mesothelioma, SV40 has been suspected to co-operate with the fibers in the neoplastic transformation or even to itself induce the onset of malignant mesothelioma in patients without expositive history. The mechanism that seems to be involved in the SV40-induced carcinogenesis process is mediated by interaction of Tag, both with p53 and Rb proteins, leading to their functional inactivation that is responsible for the removal of their inhibitory cell cycle effect which determines the increase of the number of cells entering the G1-S phase. Up to now the source of SV40 human infections has not yet been completely identified even though administration from 1957-1965 of SV40 contaminated polio vaccines is highly suspected. Horizontal infection by sexual transmission has been also hypothesized. Due to the important public health implications further investigations are required in order to establish both the source and the carcinogenetic role of Simian Virus 40 in humans.

  • Simian Virus 40 like dna sequences in human pleural mesothelioma
    Oncogene, 1994
    Co-Authors: Harvey I Pass, Michele Carbone, Paola Rizzo, M Marinetti, M Di Muzio, Daphne J Y Mew, Arthur S Levine, Antonio Procopio
    Abstract:

    Mesotheliomas are pleural, pericardial, or peritoneal neoplasms frequently associated with asbestos exposure, and it is estimated that over the next twenty years up to 80,000 new cases are expected in the USA alone. We found Simian Virus 40-like DNA sequences in 29 of 48 mesotheliomas studied (60%) and demonstrated Simian Virus large-T antigen expression in 13 of 16 specimens. The matching lung samples did not contain Simian Virus 40-like sequences; however, they contained asbestos. These findings are to our knowledge the first demonstration of a physical link between DNA Virus-like sequences and human mesothelioma. We suggest that a Simian Virus 40-like Virus may act independently or as a co-carcinogen with asbestos. Moreover, the selective large T antigen expression by mesothelioma and not by the surrounding pulmonary parenchyma may have both diagnostic and therapeutic implications.

  • Simian Virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
    Journal of virology, 1992
    Co-Authors: Michele Carbone, J Hauser, Michael P. Carty, K Rundell, Kingsley W. Dixon, Arthur S Levine
    Abstract:

    We describe a biochemical function of Simian Virus 40 small t antigen, the inhibition of Simian Virus 40 large T antigen-mediated viral DNA replication in an in vitro replication system. Our results suggest that in this system, small t antigen prevents protein phosphatase 2A-mediated activation of large T antigen.

Arthur S Levine - One of the best experts on this subject based on the ideXlab platform.

  • Simian Virus 40 like dna sequences in human pleural mesothelioma
    Oncogene, 1994
    Co-Authors: Harvey I Pass, Michele Carbone, Paola Rizzo, M Marinetti, M Di Muzio, Daphne J Y Mew, Arthur S Levine, Antonio Procopio
    Abstract:

    Mesotheliomas are pleural, pericardial, or peritoneal neoplasms frequently associated with asbestos exposure, and it is estimated that over the next twenty years up to 80,000 new cases are expected in the USA alone. We found Simian Virus 40-like DNA sequences in 29 of 48 mesotheliomas studied (60%) and demonstrated Simian Virus large-T antigen expression in 13 of 16 specimens. The matching lung samples did not contain Simian Virus 40-like sequences; however, they contained asbestos. These findings are to our knowledge the first demonstration of a physical link between DNA Virus-like sequences and human mesothelioma. We suggest that a Simian Virus 40-like Virus may act independently or as a co-carcinogen with asbestos. Moreover, the selective large T antigen expression by mesothelioma and not by the surrounding pulmonary parenchyma may have both diagnostic and therapeutic implications.

  • Simian Virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
    Journal of virology, 1992
    Co-Authors: Michele Carbone, J Hauser, Michael P. Carty, K Rundell, Kingsley W. Dixon, Arthur S Levine
    Abstract:

    We describe a biochemical function of Simian Virus 40 small t antigen, the inhibition of Simian Virus 40 large T antigen-mediated viral DNA replication in an in vitro replication system. Our results suggest that in this system, small t antigen prevents protein phosphatase 2A-mediated activation of large T antigen.

Harvey I Pass - One of the best experts on this subject based on the ideXlab platform.

  • The relationship between Simian Virus 40 and mesothelioma.
    Current opinion in pulmonary medicine, 2008
    Co-Authors: Zeyana Rivera, Harvey I Pass, Oriana Strianese, Pietro Bertino, Haining Yang, Michele Carbone
    Abstract:

    Purpose of reviewSimian Virus 40 is present in some human malignant mesotheliomas. The evidence in favor and against a pathogenic role of Simian Virus 40 in malignant mesothelioma is discussed in this review.Recent findingsWhen Simian Virus 40 is injected intracardially into hamsters, 60% develop an

  • human mesotheliomas contain the Simian Virus 40 regulatory region and large tumor antigen dna sequences
    The Journal of Thoracic and Cardiovascular Surgery, 1998
    Co-Authors: Jessica S Donington, Harvey I Pass, Paola Rizzo, Michael Nishimura, Ronald C Kennedy, Michele Carbone
    Abstract:

    Abstract Background: A cohort (20%) of patients with mesothelioma will not have an exposure to asbestos. Recently, a DNA tumor Virus (Simian Virus 40) has been shown to cause hamster mesotheliomas; we previously described Simian Virus 40–like DNA amino terminus sequences in 29 of 48 mesotheliomas. We analyzed an additional 42 mesotheliomas to determine (1) whether our initial observations were durable and (2) the extent to which the Simian Virus 40 genome is present in mesotheliomas. Methods: Genomic DNA was extracted from snap frozen mesothelioma tumor samples and from the Simian Virus 40–induced hamster mesothelioma tumor H9A. Polymerase chain reaction primers were used to amplify various Simian Virus 40 large T-antigen regions including a 105–base pair amino terminus fragment, a 281–base pair carboxyl terminus fragment, and a 310–base pair fragment of the enhancer promoter region. Endonuclease digestions and Southern blotting were used to verify the expected product. Results: Thirty of the 42 (71%) samples amplified T-antigen amino sequences, and specificity was verified by Southern hybridization. Sixteen of 42 samples (38%) amplified the appropriate size fragment for the carboxyl terminus, and digestion with BsaB1 matched that of H9A. Twenty-two of 42 samples (52%) amplified Simian Virus 40 regulatory sequences and Fok 1 digestion matched that of the hamster control tumor. Sequence analysis (4 patients) revealed 100% homology with the regulatory region of Simian Virus 40 strain 776. Conclusions: These data suggest an association between the Simian Virus 40 Virus and human mesothelioma that could be exploited for diagnostic/therapeutic options including early detection and potential vaccination strategies. (J Thorac Cardiovasc Surg 1998;116:854-9)

  • Simian Virus 40 like dna sequences in human pleural mesothelioma
    Oncogene, 1994
    Co-Authors: Harvey I Pass, Michele Carbone, Paola Rizzo, M Marinetti, M Di Muzio, Daphne J Y Mew, Arthur S Levine, Antonio Procopio
    Abstract:

    Mesotheliomas are pleural, pericardial, or peritoneal neoplasms frequently associated with asbestos exposure, and it is estimated that over the next twenty years up to 80,000 new cases are expected in the USA alone. We found Simian Virus 40-like DNA sequences in 29 of 48 mesotheliomas studied (60%) and demonstrated Simian Virus large-T antigen expression in 13 of 16 specimens. The matching lung samples did not contain Simian Virus 40-like sequences; however, they contained asbestos. These findings are to our knowledge the first demonstration of a physical link between DNA Virus-like sequences and human mesothelioma. We suggest that a Simian Virus 40-like Virus may act independently or as a co-carcinogen with asbestos. Moreover, the selective large T antigen expression by mesothelioma and not by the surrounding pulmonary parenchyma may have both diagnostic and therapeutic implications.

Paola Rizzo - One of the best experts on this subject based on the ideXlab platform.

  • human mesotheliomas contain the Simian Virus 40 regulatory region and large tumor antigen dna sequences
    The Journal of Thoracic and Cardiovascular Surgery, 1998
    Co-Authors: Jessica S Donington, Harvey I Pass, Paola Rizzo, Michael Nishimura, Ronald C Kennedy, Michele Carbone
    Abstract:

    Abstract Background: A cohort (20%) of patients with mesothelioma will not have an exposure to asbestos. Recently, a DNA tumor Virus (Simian Virus 40) has been shown to cause hamster mesotheliomas; we previously described Simian Virus 40–like DNA amino terminus sequences in 29 of 48 mesotheliomas. We analyzed an additional 42 mesotheliomas to determine (1) whether our initial observations were durable and (2) the extent to which the Simian Virus 40 genome is present in mesotheliomas. Methods: Genomic DNA was extracted from snap frozen mesothelioma tumor samples and from the Simian Virus 40–induced hamster mesothelioma tumor H9A. Polymerase chain reaction primers were used to amplify various Simian Virus 40 large T-antigen regions including a 105–base pair amino terminus fragment, a 281–base pair carboxyl terminus fragment, and a 310–base pair fragment of the enhancer promoter region. Endonuclease digestions and Southern blotting were used to verify the expected product. Results: Thirty of the 42 (71%) samples amplified T-antigen amino sequences, and specificity was verified by Southern hybridization. Sixteen of 42 samples (38%) amplified the appropriate size fragment for the carboxyl terminus, and digestion with BsaB1 matched that of H9A. Twenty-two of 42 samples (52%) amplified Simian Virus 40 regulatory sequences and Fok 1 digestion matched that of the hamster control tumor. Sequence analysis (4 patients) revealed 100% homology with the regulatory region of Simian Virus 40 strain 776. Conclusions: These data suggest an association between the Simian Virus 40 Virus and human mesothelioma that could be exploited for diagnostic/therapeutic options including early detection and potential vaccination strategies. (J Thorac Cardiovasc Surg 1998;116:854-9)

  • Simian Virus 40 like dna sequences in human pleural mesothelioma
    Oncogene, 1994
    Co-Authors: Harvey I Pass, Michele Carbone, Paola Rizzo, M Marinetti, M Di Muzio, Daphne J Y Mew, Arthur S Levine, Antonio Procopio
    Abstract:

    Mesotheliomas are pleural, pericardial, or peritoneal neoplasms frequently associated with asbestos exposure, and it is estimated that over the next twenty years up to 80,000 new cases are expected in the USA alone. We found Simian Virus 40-like DNA sequences in 29 of 48 mesotheliomas studied (60%) and demonstrated Simian Virus large-T antigen expression in 13 of 16 specimens. The matching lung samples did not contain Simian Virus 40-like sequences; however, they contained asbestos. These findings are to our knowledge the first demonstration of a physical link between DNA Virus-like sequences and human mesothelioma. We suggest that a Simian Virus 40-like Virus may act independently or as a co-carcinogen with asbestos. Moreover, the selective large T antigen expression by mesothelioma and not by the surrounding pulmonary parenchyma may have both diagnostic and therapeutic implications.

Antonio Procopio - One of the best experts on this subject based on the ideXlab platform.

  • Simian Virus 40 like dna sequences in human pleural mesothelioma
    Oncogene, 1994
    Co-Authors: Harvey I Pass, Michele Carbone, Paola Rizzo, M Marinetti, M Di Muzio, Daphne J Y Mew, Arthur S Levine, Antonio Procopio
    Abstract:

    Mesotheliomas are pleural, pericardial, or peritoneal neoplasms frequently associated with asbestos exposure, and it is estimated that over the next twenty years up to 80,000 new cases are expected in the USA alone. We found Simian Virus 40-like DNA sequences in 29 of 48 mesotheliomas studied (60%) and demonstrated Simian Virus large-T antigen expression in 13 of 16 specimens. The matching lung samples did not contain Simian Virus 40-like sequences; however, they contained asbestos. These findings are to our knowledge the first demonstration of a physical link between DNA Virus-like sequences and human mesothelioma. We suggest that a Simian Virus 40-like Virus may act independently or as a co-carcinogen with asbestos. Moreover, the selective large T antigen expression by mesothelioma and not by the surrounding pulmonary parenchyma may have both diagnostic and therapeutic implications.