The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Luigi M. De Luca - One of the best experts on this subject based on the ideXlab platform.
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Specificity of Retinoid Receptor Gene Expression in Mouse Cervical Epithelia
Endocrinology, 1994Co-Authors: Nadine Darwiche, Giulia Celli, Luigi M. De LucaAbstract:Retinoids are powerful regulators of epithelial differentiation and are essential for its maintenance. Because retinoids are necessary for cervical epithelial differentiation, they have been used as chemopreventive agents of cervical dysplasia and neoplasia. We were interested in determining whether different cervical epithelial phenotypes express specific retinoid receptors. The cervical Epithelium contains the two phenotypes, stratified squamous and Simple Columnar, which join at the squamoColumnar junction. In addition, the Simple Columnar Epithelium undergoes squamous metaplasia in response to vitamin A deficiency. Therefore, the cervical Epithelium is suitable to study the expression pattern of the retinoid receptors in the three phenotypes, Simple Columnar, stratified squamous, and squamous metaplastic, simultaneously. The distribution pattern of the major retinoic acid receptor (RAR) isoforms (alpha 1, alpha 2, beta 2, beta 3, gamma 1, and gamma 2) and retinoid-X receptors (RXR alpha, -beta, and -g...
Udensi Maduabuchi Igwebuike - One of the best experts on this subject based on the ideXlab platform.
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Histology, ultrastructure, and seasonal variations in the bulbourethral gland of the African straw‐colored fruit bat Eidolon helvum
Journal of morphology, 2020Co-Authors: Clifford Nwabugwu Abiaezute, Innocent Chima Nwaogu, Udensi Maduabuchi IgwebuikeAbstract:We studied the morphological characteristics and seasonal changes of the bulbourethral gland of Eidolon helvum in a typical African tropical environment. Forty-eight bulbourethral glands were examined using gross anatomical, histological, histochemical, and ultrastructural techniques during the early rainy, late rainy, and peak dry seasons. The pear-shaped bilateral bulbourethral glands were located extra-abdominally in the inguinal region. Trabeculae from the capsule divided the parenchyma into numerous lobules of tubuloalveolar glandular acini. The mucosa was covered by a Simple Columnar Epithelium consisting up of principal secretory cells, Columnar dense cells and basal cells, which were progressively pronounced during the dry season. The principal cells contained eosinophilic granules, which were PAS positive while the dense cells did not show affinity for the stains. The mean gross weights, acini diameters, and epithelial heights were greater during the rainy season than the dry season. Ultrastructural evaluation showed that the cytoplasm of the principal cells contained well-developed Golgi complexes, rough endoplasmic reticulum, mitochondria, and secretory vesicles of varying electron densities and sizes. The secretory vesicles were numerous during the early rainy season, decreased during the late rainy season and were scanty during the peak dry season. The Simple Columnar Epithelium observed during the rainy season was replaced by an undefined stratified Epithelium during the dry season, and this was associated with cellular degenerations and regenerations. In conclusion, E. helvum has a typical mammalian bulbourethral gland, with a unique cell type, the dense cell whose functions are not well-understood. The gland exhibits cyclical seasonal variation in structure and secretory activity; being active during the early rainy season (breeding season), and showing the lowest activity during the dry season (non-breeding season). Glandular epithelial cell renewal occurs during the dry season in preparation for the next breeding season.
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histology ultrastructure and seasonal variations in the bulbourethral gland of the african straw colored fruit bat eidolon helvum
Journal of Morphology, 2020Co-Authors: Clifford Nwabugwu Abiaezute, Innocent Chima Nwaogu, Udensi Maduabuchi IgwebuikeAbstract:We studied the morphological characteristics and seasonal changes of the bulbourethral gland of Eidolon helvum in a typical African tropical environment. Forty-eight bulbourethral glands were examined using gross anatomical, histological, histochemical, and ultrastructural techniques during the early rainy, late rainy, and peak dry seasons. The pear-shaped bilateral bulbourethral glands were located extra-abdominally in the inguinal region. Trabeculae from the capsule divided the parenchyma into numerous lobules of tubuloalveolar glandular acini. The mucosa was covered by a Simple Columnar Epithelium consisting up of principal secretory cells, Columnar dense cells and basal cells, which were progressively pronounced during the dry season. The principal cells contained eosinophilic granules, which were PAS positive while the dense cells did not show affinity for the stains. The mean gross weights, acini diameters, and epithelial heights were greater during the rainy season than the dry season. Ultrastructural evaluation showed that the cytoplasm of the principal cells contained well-developed Golgi complexes, rough endoplasmic reticulum, mitochondria, and secretory vesicles of varying electron densities and sizes. The secretory vesicles were numerous during the early rainy season, decreased during the late rainy season and were scanty during the peak dry season. The Simple Columnar Epithelium observed during the rainy season was replaced by an undefined stratified Epithelium during the dry season, and this was associated with cellular degenerations and regenerations. In conclusion, E. helvum has a typical mammalian bulbourethral gland, with a unique cell type, the dense cell whose functions are not well-understood. The gland exhibits cyclical seasonal variation in structure and secretory activity; being active during the early rainy season (breeding season), and showing the lowest activity during the dry season (non-breeding season). Glandular epithelial cell renewal occurs during the dry season in preparation for the next breeding season.
Nadine Darwiche - One of the best experts on this subject based on the ideXlab platform.
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Specificity of Retinoid Receptor Gene Expression in Mouse Cervical Epithelia
Endocrinology, 1994Co-Authors: Nadine Darwiche, Giulia Celli, Luigi M. De LucaAbstract:Retinoids are powerful regulators of epithelial differentiation and are essential for its maintenance. Because retinoids are necessary for cervical epithelial differentiation, they have been used as chemopreventive agents of cervical dysplasia and neoplasia. We were interested in determining whether different cervical epithelial phenotypes express specific retinoid receptors. The cervical Epithelium contains the two phenotypes, stratified squamous and Simple Columnar, which join at the squamoColumnar junction. In addition, the Simple Columnar Epithelium undergoes squamous metaplasia in response to vitamin A deficiency. Therefore, the cervical Epithelium is suitable to study the expression pattern of the retinoid receptors in the three phenotypes, Simple Columnar, stratified squamous, and squamous metaplastic, simultaneously. The distribution pattern of the major retinoic acid receptor (RAR) isoforms (alpha 1, alpha 2, beta 2, beta 3, gamma 1, and gamma 2) and retinoid-X receptors (RXR alpha, -beta, and -g...
Clifford Nwabugwu Abiaezute - One of the best experts on this subject based on the ideXlab platform.
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Histology, ultrastructure, and seasonal variations in the bulbourethral gland of the African straw‐colored fruit bat Eidolon helvum
Journal of morphology, 2020Co-Authors: Clifford Nwabugwu Abiaezute, Innocent Chima Nwaogu, Udensi Maduabuchi IgwebuikeAbstract:We studied the morphological characteristics and seasonal changes of the bulbourethral gland of Eidolon helvum in a typical African tropical environment. Forty-eight bulbourethral glands were examined using gross anatomical, histological, histochemical, and ultrastructural techniques during the early rainy, late rainy, and peak dry seasons. The pear-shaped bilateral bulbourethral glands were located extra-abdominally in the inguinal region. Trabeculae from the capsule divided the parenchyma into numerous lobules of tubuloalveolar glandular acini. The mucosa was covered by a Simple Columnar Epithelium consisting up of principal secretory cells, Columnar dense cells and basal cells, which were progressively pronounced during the dry season. The principal cells contained eosinophilic granules, which were PAS positive while the dense cells did not show affinity for the stains. The mean gross weights, acini diameters, and epithelial heights were greater during the rainy season than the dry season. Ultrastructural evaluation showed that the cytoplasm of the principal cells contained well-developed Golgi complexes, rough endoplasmic reticulum, mitochondria, and secretory vesicles of varying electron densities and sizes. The secretory vesicles were numerous during the early rainy season, decreased during the late rainy season and were scanty during the peak dry season. The Simple Columnar Epithelium observed during the rainy season was replaced by an undefined stratified Epithelium during the dry season, and this was associated with cellular degenerations and regenerations. In conclusion, E. helvum has a typical mammalian bulbourethral gland, with a unique cell type, the dense cell whose functions are not well-understood. The gland exhibits cyclical seasonal variation in structure and secretory activity; being active during the early rainy season (breeding season), and showing the lowest activity during the dry season (non-breeding season). Glandular epithelial cell renewal occurs during the dry season in preparation for the next breeding season.
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histology ultrastructure and seasonal variations in the bulbourethral gland of the african straw colored fruit bat eidolon helvum
Journal of Morphology, 2020Co-Authors: Clifford Nwabugwu Abiaezute, Innocent Chima Nwaogu, Udensi Maduabuchi IgwebuikeAbstract:We studied the morphological characteristics and seasonal changes of the bulbourethral gland of Eidolon helvum in a typical African tropical environment. Forty-eight bulbourethral glands were examined using gross anatomical, histological, histochemical, and ultrastructural techniques during the early rainy, late rainy, and peak dry seasons. The pear-shaped bilateral bulbourethral glands were located extra-abdominally in the inguinal region. Trabeculae from the capsule divided the parenchyma into numerous lobules of tubuloalveolar glandular acini. The mucosa was covered by a Simple Columnar Epithelium consisting up of principal secretory cells, Columnar dense cells and basal cells, which were progressively pronounced during the dry season. The principal cells contained eosinophilic granules, which were PAS positive while the dense cells did not show affinity for the stains. The mean gross weights, acini diameters, and epithelial heights were greater during the rainy season than the dry season. Ultrastructural evaluation showed that the cytoplasm of the principal cells contained well-developed Golgi complexes, rough endoplasmic reticulum, mitochondria, and secretory vesicles of varying electron densities and sizes. The secretory vesicles were numerous during the early rainy season, decreased during the late rainy season and were scanty during the peak dry season. The Simple Columnar Epithelium observed during the rainy season was replaced by an undefined stratified Epithelium during the dry season, and this was associated with cellular degenerations and regenerations. In conclusion, E. helvum has a typical mammalian bulbourethral gland, with a unique cell type, the dense cell whose functions are not well-understood. The gland exhibits cyclical seasonal variation in structure and secretory activity; being active during the early rainy season (breeding season), and showing the lowest activity during the dry season (non-breeding season). Glandular epithelial cell renewal occurs during the dry season in preparation for the next breeding season.
Francis Michael Clarke - One of the best experts on this subject based on the ideXlab platform.
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Expression and localisation of thioredoxin in mouse reproductive tissues during the oestrous cycle.
Molecular reproduction and development, 2001Co-Authors: Leisa Jane Osborne, Kathryn Fay Tonissen, Vivian Tang, Francis Michael ClarkeAbstract:Thioredoxin expression within the reproductive tissues of the female mouse was analysed during the oestrous cycle stages of dioestrus, oestrus and metoestrus by Western blot analyses and immunocytochemistry. From Western blot analyses the expression of thioredoxin was found to be increased in oestrus compared to dioestrus and metoestrus. Localisation of thioredoxin within the reproductive organs of the mouse during the oestrous cycle has shown that the expression of thioredoxin is specific for distinct areas within the reproductive organs. These areas are the stratified squamous Epithelium of the vagina, the Simple Columnar Epithelium and the uterine glands of the uterus, the ciliated Columnar Epithelium of the oviduct, the corpus lutea, the interstitial cells and the secondary follicles of the ovary. The discrete cellular localisation and oestrous dependence of thioredoxin expression are suggestive of specific roles in various reproductive processes.