The Experts below are selected from a list of 207849 Experts worldwide ranked by ideXlab platform
Mustafa Culha - One of the best experts on this subject based on the ideXlab platform.
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Surface-Enhanced Raman Scattering for Label-Free Living Single Cell Analysis
Analytical Chemistry, 2017Co-Authors: Gamze Kuku, Mine Altunbek, Mustafa CulhaAbstract:Single Cell Analysis is an active research area with the hope that Cellular process can be deciphered from a Single living Cell other than a Cell population. Surface enhanced Raman scattering (SERS) has been increasingly investigated for Single Cell Analysis with its ability to provide information about real-time dynamics of molecular processes taking place in living Cells, especially upon external stimulation, in a contactless, noninvasive, and nondestructive way. In this perspective, the fundamental concepts of Single Cell-SERS Analysis including origin of spectral bands and experimental parameters for spectral reproducibility are summarized along with the recent developments.
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Gold Nanoparticles in Single-Cell Analysis for Surface Enhanced Raman Scattering
Molecules, 2016Co-Authors: Mine Altunbek, Gamze Kuku, Mustafa CulhaAbstract:The need for new therapeutic approaches in the treatment of challenging diseases such as cancer, which often consists of a highly heterogeneous and complex population of Cells, brought up the idea of analyzing Single Cells. The development of novel techniques to analyze Single Cells has been intensively studied to fully understand specific alternations inducing abnormalities in Cellular function. One of the techniques used for Single Cell Analysis is surface-enhanced Raman spectroscopy (SERS) in which a noble metal nanoparticle is used to enhance Raman scattering. Due to its low toxicity and biocompatibility, gold nanoparticles (AuNPs) are commonly preferred as SERS substrates in Single Cell Analysis. The intraCellular uptake, localization and toxicity issues of AuNPs are the critical points for interpretation of data since the obtained SERS signals originate from molecules in close vicinity to AuNPs that are taken up by the Cells. In this review, the AuNP–living Cell interactions, Cellular uptake and toxicity of AuNPs in relation to their physicochemical properties, and surface-enhanced Raman scattering from Single Cells are discussed.
Norbert Jakubowski - One of the best experts on this subject based on the ideXlab platform.
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Single-Cell Analysis by use of ICP-MS
Journal of Analytical Atomic Spectrometry, 2020Co-Authors: Sarah Theiner, Konrad Loehr, Gunda Koellensperger, Larissa Mueller, Norbert JakubowskiAbstract:This tutorial review article is highlighting the fundamentals, instrumentation, and most recent trends of Single-Cell Analysis by use of inductively coupled plasma-mass spectrometry (ICP-MS). It is shown that metals and hetero-elements being intrinsically present in Cells, taken up by Cells (for instance engineered metallic nanoparticles) or binding to a Cell can be detected qualitatively by existing ICP-MS technologies on a Single Cell level. Adding a quantitative dimension to Single-Cell Analysis by (laser ablation-) ICP-MS requires dedicated calibration and validation strategies, which are currently being established and are being critically discussed. In a tutorial part, the ICP-MS instruments, the measurement conditions, and the sample introduction and preparation techniques are introduced. The application section focuses on the state-of-the-art of Single-Cell Analysis in suspension, using laser ablation or (imaging) mass cytometry. Finally, future trends are critically assessed.
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Current trends in Single Cell Analysis
Analytical and Bioanalytical Chemistry, 2014Co-Authors: Petra S. Dittrich, Norbert JakubowskiAbstract:Single Cell Analysis is a rapidly emerging research field that fascinates researchers from various disciplines: biologists, because it may reveal insights into fundamental aspects like evolution, Cell adaptation, and Cell differentiation; medical doctors and pharmacists, because it may have an impact in the understanding of diseases such as cancer, the functioning of the immune system, or the emergence of antibiotic resistances; biotechnologists, because it may further advance evolutionary biotechnology; analytical chemists, because it forces any analytical method to its limits. Single Cells provide an extremely difficult matrix for Analysis as they are small in size (approximately 1–50 μm) and volume (1 fL for a bacterial Cell!) and they consist of many different types of biomolecules with concentrations in a very large dynamic range (few copies of a molecule next to concentrations of mM). Moreover, a living Cell is not a stable “mixture,” it grows and, thereby, exhibits different Cell cycle stages, it has a metabolism, and it may respond to slight variations in the external chemical, physical, or mechanical environment. For these reasons, suspensions of Cells are used in classic analytical approaches to analyze Cellular content. However, such types of bulk measurements provide average values of Cell populations but obscure individual behavior of the Cells. Even isogenic Cells (derived from the same parent Cell) can differ significantly because each Single Cell is embedded in a local microenvironment, interacts with neighboring Cells, or can develop in a stochastic way differently from others (intrinsic noise). These heterogeneities can be observed on all levels of biomolecules such as the transcriptome, proteome, and the metabolome level. However, every type of molecule requires again a different analytical method. Current analytical methods for Single Cell Analysis are either based on non-nvasive, mainly optical methods (“biological” Analysis) or on the chemical Analysis of lysate derived from individual Cells. Noninvasive methods allow the observation of living Cells, also over longer time scales, and have been routinely performed by means of fluorescence microscopy and related techniques. They address typically only one or very few target molecules, but are highly specific and sensitive. More recent approaches use label-free analytical methods, e.g., Raman spectroscopy and impedance measurements, with the big advantage that tedious labeling strategies are not required any more and Cells can be observed without any intervention in their nature. On the other hand, the chemical Analysis of Cell lysates allows detailed quantification of target molecules, e.g., by mass spectrometry [1] or immunoassays [2]. Beyond the tremendous increase of the sensitivity of many analytical instruments in recent years, the advances in Cell and fluid handling attributable to microfluidics technology has pushed the field of Single-Cell Analysis to yet another level. Microfluidics provides solutions to capture and/or encapsulate Single Cells in traps or droplets, and to control the environment at an unprecedented accuracy so that reliable studies of individual Cell response become feasible. In addition, aliquoting of Cell lysates on microfluidic platforms is within reach. Microfluidic platforms routinely made of transparent materials such as polymers or glass are very well suited for microscopy and related techniques. In recent years, Published in the topical collection Single Cell Analysis with guest editors Petra Dittrich and Norbert Jakubowski.
Mine Altunbek - One of the best experts on this subject based on the ideXlab platform.
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Surface-Enhanced Raman Scattering for Label-Free Living Single Cell Analysis
Analytical Chemistry, 2017Co-Authors: Gamze Kuku, Mine Altunbek, Mustafa CulhaAbstract:Single Cell Analysis is an active research area with the hope that Cellular process can be deciphered from a Single living Cell other than a Cell population. Surface enhanced Raman scattering (SERS) has been increasingly investigated for Single Cell Analysis with its ability to provide information about real-time dynamics of molecular processes taking place in living Cells, especially upon external stimulation, in a contactless, noninvasive, and nondestructive way. In this perspective, the fundamental concepts of Single Cell-SERS Analysis including origin of spectral bands and experimental parameters for spectral reproducibility are summarized along with the recent developments.
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Gold Nanoparticles in Single-Cell Analysis for Surface Enhanced Raman Scattering
Molecules, 2016Co-Authors: Mine Altunbek, Gamze Kuku, Mustafa CulhaAbstract:The need for new therapeutic approaches in the treatment of challenging diseases such as cancer, which often consists of a highly heterogeneous and complex population of Cells, brought up the idea of analyzing Single Cells. The development of novel techniques to analyze Single Cells has been intensively studied to fully understand specific alternations inducing abnormalities in Cellular function. One of the techniques used for Single Cell Analysis is surface-enhanced Raman spectroscopy (SERS) in which a noble metal nanoparticle is used to enhance Raman scattering. Due to its low toxicity and biocompatibility, gold nanoparticles (AuNPs) are commonly preferred as SERS substrates in Single Cell Analysis. The intraCellular uptake, localization and toxicity issues of AuNPs are the critical points for interpretation of data since the obtained SERS signals originate from molecules in close vicinity to AuNPs that are taken up by the Cells. In this review, the AuNP–living Cell interactions, Cellular uptake and toxicity of AuNPs in relation to their physicochemical properties, and surface-enhanced Raman scattering from Single Cells are discussed.
Gamze Kuku - One of the best experts on this subject based on the ideXlab platform.
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Surface-Enhanced Raman Scattering for Label-Free Living Single Cell Analysis
Analytical Chemistry, 2017Co-Authors: Gamze Kuku, Mine Altunbek, Mustafa CulhaAbstract:Single Cell Analysis is an active research area with the hope that Cellular process can be deciphered from a Single living Cell other than a Cell population. Surface enhanced Raman scattering (SERS) has been increasingly investigated for Single Cell Analysis with its ability to provide information about real-time dynamics of molecular processes taking place in living Cells, especially upon external stimulation, in a contactless, noninvasive, and nondestructive way. In this perspective, the fundamental concepts of Single Cell-SERS Analysis including origin of spectral bands and experimental parameters for spectral reproducibility are summarized along with the recent developments.
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Gold Nanoparticles in Single-Cell Analysis for Surface Enhanced Raman Scattering
Molecules, 2016Co-Authors: Mine Altunbek, Gamze Kuku, Mustafa CulhaAbstract:The need for new therapeutic approaches in the treatment of challenging diseases such as cancer, which often consists of a highly heterogeneous and complex population of Cells, brought up the idea of analyzing Single Cells. The development of novel techniques to analyze Single Cells has been intensively studied to fully understand specific alternations inducing abnormalities in Cellular function. One of the techniques used for Single Cell Analysis is surface-enhanced Raman spectroscopy (SERS) in which a noble metal nanoparticle is used to enhance Raman scattering. Due to its low toxicity and biocompatibility, gold nanoparticles (AuNPs) are commonly preferred as SERS substrates in Single Cell Analysis. The intraCellular uptake, localization and toxicity issues of AuNPs are the critical points for interpretation of data since the obtained SERS signals originate from molecules in close vicinity to AuNPs that are taken up by the Cells. In this review, the AuNP–living Cell interactions, Cellular uptake and toxicity of AuNPs in relation to their physicochemical properties, and surface-enhanced Raman scattering from Single Cells are discussed.
Helene Andersson-svahn - One of the best experts on this subject based on the ideXlab platform.
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Single-Cell Analysis : methods and protocols
2012Co-Authors: Sara Lindström, Helene Andersson-svahnAbstract:1. Introduction: Why Analyze Single Cells? Dino Di Carlo, Henry Tat Kwong Tse, and Daniel R. Gossett Part I: Conventional Methods 2. Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview Sara Lindstrom 3. Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry Robert B. Brown, Johannes A. Hewel, Andrew Emili, and Julie Audet 4. Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts Ward De Spiegelaere, Muriel Filliers, and Ann Van Soom Part II: Miniaturized Solutions 5. Single-Cell Culture in Microwells Sara Lindstrom and Helene Andersson-Svahn 6. Iso-Dielectric Separation and Analysis of Cells Michael D. Vahey and Joel Voldman 7. Single Cell Electroporation Using Microfluidic Devices Severine Le Gac and Albert van den Berg 8. Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array Chi Zhang 9. Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay Sara Henriksson and Mats Nilsson 10. Droplet Microfluidics for Single-Cell Analysis Eric Brouzes 11. Screening of Antigen-Specific Antibody Secreting Cells Hiroyuki Kishi, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, and Atsushi Muraguchi 12. Analysis of Single Eukaryotic Cells Using Raman Tweezers Elsa Correia Faria and Peter Gardner 13. Single-Cell Microinjection Technologies Yan Zhang 14. Ultrasonic Manipulation of Single Cells Martin Wiklund and Bjorn Onfelt Part III: Reviews on Chosen Subjects Within the Field 15. Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices Soo Hyeon Kim, Dominique Fourmy, and Teruo Fujii 16. Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses Ayca Yalcin, Yvonne J. Yamanaka, and J. Christopher Love
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A microwell array device with integrated microfluidic components for enhanced Single-Cell Analysis.
ELECTROPHORESIS, 2009Co-Authors: Sara Lindström, Kiichiroh Mori, Toshiro Ohashi, Helene Andersson-svahnAbstract:Increasing awareness of the importance of Cell heterogeneity in many biological and medical contexts is prompting increasing interest in systems that allow Single-Cell Analysis rather than conventi ...
