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Julian Chen - One of the best experts on this subject based on the ideXlab platform.
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Insight into watery saliva proteomes of the grain aphid, Sitobion Avenae.
Archives of Insect Biochemistry and Physiology, 2020Co-Authors: Yong Zhang, Frédéric Francis, Xiaobei Liu, Julian ChenAbstract:The grain aphid, Sitobion Avenae, is an economically important cereal pest worldwide. Aphid saliva plays an essential role in the interaction between aphids and their host plants. However, limited information is available regarding the proteins found in the saliva of S. Avenae. Here, the watery saliva proteins from S. Avenae were collected in an artificial diet and identified using a liquid chromatography-mass spectrometry/mass spectrometry analysis. A total of 114 proteins were identified in S. Avenae saliva, including several enzymes, binding proteins, and putative effectors, as well as other proteins with unknown functions. In comparison with salivary proteins from nine other aphid species, the most striking feature of the salivary protein from S. Avenae was the different patterns of protein functions. Several orthologous proteins secreted by other aphid species such as glucose dehydrogenase, elongation factors, and effector C002 were also detected in S. Avenae saliva and speculated to play a significant role in aphid-plant interactions. These results provide further insight into the molecular basis between aphids and cereal plant interactions.
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watery saliva secreted by the grain aphid Sitobion Avenae stimulates aphid resistance in wheat
Journal of Agricultural and Food Chemistry, 2017Co-Authors: Yong Zhang, Frédéric Francis, Jia Fan, Julian ChenAbstract:Infestation with Sitobion Avenae induces localized defense responses in wheat; in this study, the role of S. Avenae watery saliva in resistance induction was examined by infiltrating aphid saliva into wheat leaves. After feeding S. Avenae on an artificial diet for 48 h, we first collected watery saliva from them and then separated the salivary proteins using one-dimensional gel electrophoresis. Gene expression studies showed that infiltration of S. Avenae watery saliva in wheat leaves induced a strong salicylic acid-responsive defense but moderate jasmonic acid-dependent defense. Feeding on wheat leaves infiltrated with aphid saliva, compared with untreated leaves, significantly decreased the number of nymphs produced per day and the intrinsic rate of increase of the population of S. Avenae. In a choice test against untreated wheat, saliva-infiltrated wheat had repellent effects on aphids. Additionally, electrical penetration graph results showed that the feeding behavior of S. Avenae on saliva-treated wh...
Hui-yan Zhao - One of the best experts on this subject based on the ideXlab platform.
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Ecological impact of a secondary bacterial symbiont on the clones of Sitobion Avenae (Fabricius) (Hemiptera: Aphididae).
Scientific Reports, 2017Co-Authors: Chen Luo, Kun Luo, Linqin Meng, Bin Wan, Hui-yan ZhaoAbstract:Many insects harbor heritable endosymbionts, whether obligatory or facultative, and the role of facultative endosymbionts in shaping the phenotype of these species has become increasingly important. However, little is known about whether micro-injected endosymbionts can have any effects on aphid clones, which was measured using various ecological parameters. We examined the effects between symbiotic treatments and the vital life history traits generated by Regiella insecticola on the life table parameters of Sitobion Avenae. The results showed that R. insecticola can decrease the intrinsic rate of increase (r), the finite rate of increase (λ) and birth rate and can increase the mean generation times (T) of S. Avenae clones, suggesting that R. insecticola may decelerate the normal development of the hosts. No significant differences of these parameters were observed between the examined Sitobion Avenae clones, and the symbiont treatment by genotype interaction affected only the net reproduction rate R0, pre-adult duration and total longevity but not the other parameters. Additionally, a population projection showed that R. insecticola decelerated the growth of the S. Avenae clones. The evocable effects of R. insecticola on the S. Avenae clones may have significant ramifications for the control of S. Avenae populations under field/natural conditions.
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The infection frequencies and dynamics of three secondary endosymbionts in the laboratory environments on Sitobion Avenae (Fabricius) as determined by long PCR
Journal of Asia-Pacific Entomology, 2016Co-Authors: Chen Luo, Kun Luo, Yue-yue Tao, Hui-yan ZhaoAbstract:Abstract Stable infections of maternally transmitted secondary endosymbionts in aphids are frequently found in field populations; however, whether this phenomenon changes in artificial lab conditions has not been clear. To investigate, (1): we collected Sitobion Avenae (Fabricius) from six different regions and detected the three common secondary endosymbionts (Regiella insecticola, Hamiltonella defensa and Serratia symbiotica); (2): we raised Sitobion Avenae from Chuzhou, Anhui for 14 months in the laboratory, and analyzed these aphids' DNA using the long PCR protocol after the 1st month, 3rd month, 5th month, 7th month, 9th month, 11th month, 12th month, 13th month and 14th month. The results showed that S. symbiotica and R. insecticola could be detected in all aphid populations, while H. defense were detected in three locations. After reared in the lab, the infection frequencies of Serratia symbiotica did not change across the 14 study months, but the infection frequencies of Regiella insecticola after the 9th month and of Hamiltonella defensa after the 11th month decreased significantly. Additionally, the results show that facultative endosymbiont infections may decline in artificial lab conditions, suggesting that temporal changes of endosymbionts may occur within the host under different environmental pressures. This result may partially explain why different geographic populations harbor a diverse variety of endosymbionts. The results of this study can be helpful in understanding the stability of secondary endosymbionts in Sitobion Avenae under long-term reproduction in controlled laboratory conditions.
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Life table analysis of the performance of aphid Sitobion Avenae (Homoptera: Aphididae) nymphs exposed to a static magnetic field
Archives of Biological Sciences, 2013Co-Authors: Huan-huan Gao, Walter Monika, Zhu Cao, Hui-yan ZhaoAbstract:Using the age-stage two-sex life table, this work was undertaken in order to determine the effect of static magnetic fields (SMFs) at two flux densities (0.176T and 0.065T) applied at increasing times of duration (0.25, 0.5, 1 and 2 h) on the development, fecundity and reproduction of the aphid, Sitobion Avenae. Exposed nymphs had a statistically significant shortened first instar period and adult longevity and prolonged fourth instar periods compared to controls. There were significant differences in the population parameters for two exposure combinations, 0.176T for 0.5 h and 0.065T for 1 h. The intrinsic rate of increase (r), net reproductive rate (R0) and mean generation time (T) were 0.1165, 3.5 and 11.7 days, respectively, 0.176 T for 0.5 h and -0.0198, 0.7 and 11.8 days, respectively, 0.065T for 1 h. We therefore recommend using the age-stage, two-sex life table to study the effect of the static magnetic field on development and growth of the aphid, Sitobion Avenae.
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EFFECT OF STATIC MAGNETIC FIELDS (SMF) ON THE VIABILITY AND FECUNDITY OF APHID Sitobion Avenae (HOMOPTERA: APHIDIDAE) UNDER LABORATORY CONDITIONS
Archives of Biological Sciences, 2012Co-Authors: Huan-huan Gao, Hui-yan Zhao, Walter MonikaAbstract:The present study examines the effect of static magnetic fields (SMF) on aphids. The effect of a field of moderateintensity (1 mT-1 T) SMF on the life of Sitobion Avenae was estimated using the age-stage, two-sex life table. Static magnetic fields were 0.065 T and 0.176 T and exposure was for 0, 15, 30, 60, and 120 min. The results show that the development, mortality, and fecundity of S. Avenae were significantly affected by SMF in the second generation after exposure to 0.176 T for 30 min and to 0.065 T for 60 min in comparison to the control group. We concluded that SMF affects the development and reproduction of Sitobion Avenae.
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Life table evaluation of survival and reproduction of the aphid, Sitobion Avenae, exposed to cadmium.
Journal of Insect Science, 2012Co-Authors: Huan-huan Gao, Hui-yan Zhao, Ming-ming DengAbstract:The effects of cadmium (Cd) on the development, fecundity, and reproduction of the grain aphid, Sitobion Avenae Fabricius (Hemiptera: Aphididae) were estimated by constructing a life table of S. Avenae exposed to Cd. The concentrations of Cd in the soil were as follows: 0, 10, 20, 40, 80, and 160 mg/kg. The correlation analysis of the Cd concentration in soil and wheat revealed that the amount in the wheat increased with the increase of Cd concentrations in soil. The results indicated that, the latter part of the reproduction period was significantly affected by Cd, according to the curve of the total survival rate (lx). The net reproductive rate (R 0), innate capacity of increase (r), and finite rate of increase (λ) of S. Avenae all decreased under the stress of Cd, and were lowest at a Cd concentration of 20 mg/kg. Cd also negatively affected fecundity and mx (the number of offspring produced by an individual female). At 20 mg/kg, the decline of them was most obvious. In conclusion, survival and reproduction of S. Avenae were inhibited under the treatment of the heavy metal Cd. Sitobion Avenae was more sensitive to Cd at concentration of 20 mg/kg compared to the other concentrations. This concentration can be used to examine the mechanisms behind population genetics and biological mutation of S. Avenae when exposed to heavy metal.
Yong Zhang - One of the best experts on this subject based on the ideXlab platform.
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Insight into watery saliva proteomes of the grain aphid, Sitobion Avenae.
Archives of Insect Biochemistry and Physiology, 2020Co-Authors: Yong Zhang, Frédéric Francis, Xiaobei Liu, Julian ChenAbstract:The grain aphid, Sitobion Avenae, is an economically important cereal pest worldwide. Aphid saliva plays an essential role in the interaction between aphids and their host plants. However, limited information is available regarding the proteins found in the saliva of S. Avenae. Here, the watery saliva proteins from S. Avenae were collected in an artificial diet and identified using a liquid chromatography-mass spectrometry/mass spectrometry analysis. A total of 114 proteins were identified in S. Avenae saliva, including several enzymes, binding proteins, and putative effectors, as well as other proteins with unknown functions. In comparison with salivary proteins from nine other aphid species, the most striking feature of the salivary protein from S. Avenae was the different patterns of protein functions. Several orthologous proteins secreted by other aphid species such as glucose dehydrogenase, elongation factors, and effector C002 were also detected in S. Avenae saliva and speculated to play a significant role in aphid-plant interactions. These results provide further insight into the molecular basis between aphids and cereal plant interactions.
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watery saliva secreted by the grain aphid Sitobion Avenae stimulates aphid resistance in wheat
Journal of Agricultural and Food Chemistry, 2017Co-Authors: Yong Zhang, Frédéric Francis, Jia Fan, Julian ChenAbstract:Infestation with Sitobion Avenae induces localized defense responses in wheat; in this study, the role of S. Avenae watery saliva in resistance induction was examined by infiltrating aphid saliva into wheat leaves. After feeding S. Avenae on an artificial diet for 48 h, we first collected watery saliva from them and then separated the salivary proteins using one-dimensional gel electrophoresis. Gene expression studies showed that infiltration of S. Avenae watery saliva in wheat leaves induced a strong salicylic acid-responsive defense but moderate jasmonic acid-dependent defense. Feeding on wheat leaves infiltrated with aphid saliva, compared with untreated leaves, significantly decreased the number of nymphs produced per day and the intrinsic rate of increase of the population of S. Avenae. In a choice test against untreated wheat, saliva-infiltrated wheat had repellent effects on aphids. Additionally, electrical penetration graph results showed that the feeding behavior of S. Avenae on saliva-treated wh...
Xiaoqin Shi - One of the best experts on this subject based on the ideXlab platform.
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Developmental time and fecundity for Sitobion Avenae clones on wheat, barley and oat.
2018Co-Authors: Xianliang Huang, Deguang Liu, Xiaoning Cui, Xiaoqin ShiAbstract:Developmental time and fecundity for Sitobion Avenae clones on wheat, barley and oat.
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Frequency and duration# (mean ± SE) of probing behaviors for Sitobion Avenae clones on wheat, barley and oat.
2018Co-Authors: Xianliang Huang, Deguang Liu, Xiaoning Cui, Xiaoqin ShiAbstract:Frequency and duration# (mean ± SE) of probing behaviors for Sitobion Avenae clones on wheat, barley and oat.
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Selection differentials and gradients for probing behavioral plasticities of Sitobion Avenae clones on two alternative plants (i.e., barley and oat).
2018Co-Authors: Xianliang Huang, Deguang Liu, Xiaoning Cui, Xiaoqin ShiAbstract:Selection differentials and gradients for probing behavioral plasticities of Sitobion Avenae clones on two alternative plants (i.e., barley and oat).
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Estimates of variance components for probing behavioral plasticities of Sitobion Avenae clones showing main effects of plant, clone and interactions.
2018Co-Authors: Xianliang Huang, Deguang Liu, Xiaoning Cui, Xiaoqin ShiAbstract:Estimates of variance components for probing behavioral plasticities of Sitobion Avenae clones showing main effects of plant, clone and interactions.
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Probing behaviors and their plasticity for the aphid Sitobion Avenae on three alternative host plants - Fig 3
2018Co-Authors: Xianliang Huang, Deguang Liu, Xiaoning Cui, Xiaoqin ShiAbstract:Spearman (a) and Hoeffding (b) association coefficients between probing behaviors and their plasticities for Sitobion Avenae clones (Np, non-probing; C, pathway phase; E1, phloem salivation; E2, phloem ingestion; G, xylem ingestion; F, stylet derailment. *, P < 0.05; **, P < 0.01; ***, P < 0.001).
Frédéric Francis - One of the best experts on this subject based on the ideXlab platform.
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Insight into watery saliva proteomes of the grain aphid, Sitobion Avenae.
Archives of Insect Biochemistry and Physiology, 2020Co-Authors: Yong Zhang, Frédéric Francis, Xiaobei Liu, Julian ChenAbstract:The grain aphid, Sitobion Avenae, is an economically important cereal pest worldwide. Aphid saliva plays an essential role in the interaction between aphids and their host plants. However, limited information is available regarding the proteins found in the saliva of S. Avenae. Here, the watery saliva proteins from S. Avenae were collected in an artificial diet and identified using a liquid chromatography-mass spectrometry/mass spectrometry analysis. A total of 114 proteins were identified in S. Avenae saliva, including several enzymes, binding proteins, and putative effectors, as well as other proteins with unknown functions. In comparison with salivary proteins from nine other aphid species, the most striking feature of the salivary protein from S. Avenae was the different patterns of protein functions. Several orthologous proteins secreted by other aphid species such as glucose dehydrogenase, elongation factors, and effector C002 were also detected in S. Avenae saliva and speculated to play a significant role in aphid-plant interactions. These results provide further insight into the molecular basis between aphids and cereal plant interactions.
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Protein elicitor PeaT1 enhanced resistance against aphid (Sitobion Avenae) in wheat
Pest Management Science, 2019Co-Authors: Wang Shuangchao, Xiufen Yang, Frédéric Francis, Dewen QiuAbstract:BACKGROUND Sitobion Avenae, a dominant aphid in wheat that causes huge annual losses in agriculture, is mainly controlled using chemical pesticides. In this study, we investigated a protein elicitor, PeaT, for its induction of the defense response in wheat against Sitobion Avenae. RESULTS Intrinsic rates of increase in second and third generations of S. Avenae decreased in the PeaT1 (second generation 0.31 ± 0.01, third generation 0.28 ± 0.01) treatment compared with controls (second generation 0.28 ± 0.01, third generation 0.26 ± 0.01). S. Avenae preferred to colonize control rather than PeaT1-treated wheat seedlings in a host selection test. PeaT1-treated wheat leaves possessed more trichomes and wax that formed a disadvantageous surface environment for S. Avenae. Both salicylic acid (SA) and jasmonic acid (JA) accumulated significantly in PeaT1-treated wheat seedlings. CONCLUSION These results showed that PeaT1 modified physical surface structures in wheat to reduce reproduction and deter colonization by S. Avenae. SA and JA were involved in the induced physical defense process. This study provided evidence for use of PeaT1 as a 'vaccine' to protect wheat from Sitobion Avenae. © 2019 Society of Chemical Industry.
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watery saliva secreted by the grain aphid Sitobion Avenae stimulates aphid resistance in wheat
Journal of Agricultural and Food Chemistry, 2017Co-Authors: Yong Zhang, Frédéric Francis, Jia Fan, Julian ChenAbstract:Infestation with Sitobion Avenae induces localized defense responses in wheat; in this study, the role of S. Avenae watery saliva in resistance induction was examined by infiltrating aphid saliva into wheat leaves. After feeding S. Avenae on an artificial diet for 48 h, we first collected watery saliva from them and then separated the salivary proteins using one-dimensional gel electrophoresis. Gene expression studies showed that infiltration of S. Avenae watery saliva in wheat leaves induced a strong salicylic acid-responsive defense but moderate jasmonic acid-dependent defense. Feeding on wheat leaves infiltrated with aphid saliva, compared with untreated leaves, significantly decreased the number of nymphs produced per day and the intrinsic rate of increase of the population of S. Avenae. In a choice test against untreated wheat, saliva-infiltrated wheat had repellent effects on aphids. Additionally, electrical penetration graph results showed that the feeding behavior of S. Avenae on saliva-treated wh...
