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J Opudaasibo - One of the best experts on this subject based on the ideXlab platform.
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screening of extracts of zanthoxylum chalybeum and warburgia ugandensis for activity against measles virus swartz and edmonston strains in vitro
African Health Sciences, 2002Co-Authors: Deogracious Olila, J OpudaasiboAbstract:A large proportion of the population in Uganda still relies on the use of plant extracts for treatment of various ailments. This study tested the claimed efficacy of some plants in the treatment of measles. In vitro antiviral assays were performed on extracts of two medicinal plants (Warburgia ugandensis and Zanthoxylum chalybeum) using measles virus (Edmonston and Swartz strains) as the test organisms. The assays performed were the neutralisation tests and the plaque reduction assays. Of the two plants Z. chalybeum had demonstrable in vitro antiviral activity in the seed extracts (titer reduction factor [TRF]: 100, for the ethanolic extract). The in vitro antiviral activity of the seed extracts was demonstrated to be due to compound 27–135D (TRF=1000), which was characterized by 1H-NMR spectroscopy as the alkaloid Skimmianine. Skimmianine had minimal toxicity to VERO cell lines. The petroleum ether extracts and the ethanolic extracts of Warburgia ugandensis had no inhibitory effect on cytopathic effect (CPE) formation, especially at the maximal non-toxic dose (MNTD). The extracts of W. ugandensis were highy toxic to VERO cell lines. The TRF values for the stem bark extracts of W. ugandensis were: water extract, 10; ethanolic extract, 1; fraction 27–163D, 100., which were regarded to be too low. Seed extracts of Z.chalybeum therefore probably cure measles due to the antiviral effect of Skimmianine. It is not clear how extracts of W. ugandensis produce a beneficial response in measles disease, if at all.
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screening of extracts of zanthoxylum chalybeum and warburgia ugandensis for activity against measles virus swartz and edmonston strains in vitro
African Health Sciences, 2002Co-Authors: Deogracious Olila, J OpudaasiboAbstract:A large proportion of the population in Uganda still relies on the use of plant extracts for treatment of various ailments. This study tested the claimed efficacy of some plants in the treatment of measles. In vitro antiviral assays were performed on extracts of two medicinal plants (Warburgia ugandensis and Zanthoxylum chalybeum) using measles virus (Edmonston and Swartz strains) as the test organisms. The assays performed were the neutralisation tests and the plaque reduction assays. Of the two plants Z. chalybeum had demonstrable in vitro antiviral activity in the seed extracts (titer reduction factor [TRF]: 100, for the ethanolic extract). The in vitro antiviral activity of the seed extracts was demonstrated to be due to compound 27–135D (TRF=1000), which was characterized by 1H-NMR spectroscopy as the alkaloid Skimmianine. Skimmianine had minimal toxicity to VERO cell lines. The petroleum ether extracts and the ethanolic extracts of Warburgia ugandensis had no inhibitory effect on cytopathic effect (CPE) formation, especially at the maximal non-toxic dose (MNTD). The extracts of W. ugandensis were highy toxic to VERO cell lines. The TRF values for the stem bark extracts of W. ugandensis were: water extract, 10; ethanolic extract, 1; fraction 27–163D, 100., which were regarded to be too low. Seed extracts of Z.chalybeum therefore probably cure measles due to the antiviral effect of Skimmianine. It is not clear how extracts of W. ugandensis produce a beneficial response in measles disease, if at all.
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antibacterial and antifungal activities of extracts of zanthoxylum chalybeum and warburgia ugandensis ugandan medicinal plants
African Health Sciences, 2001Co-Authors: Deogracious Olila, J OpudaasiboAbstract:Measles is a killer disease of children in Uganda. The treatment of the disease is mainly directed at the secondary microbial infections. A large proportion of the population in Uganda still relies on the use of herbal remedies, which have been claimed to produce beneficial responses. In this study, the efficacy of Warburgia ugandensis and Zanthoxylum chalybeum against common bacteria and fungi was investigated. Bactericidal and antifungal assays were done using extracts derived from Z. chalybeum and W. ugandensis (agar well diffusion, disc diffusion and colony count assays). All extracts (ethanolic, petroleum ether and aqueous) of Z. chalybeum did not show antimicrobial activity. Phytochemical investigations of Zanthoxylum chalybeum (seed) yielded a pure crystalline alkaloid (27–135D) which was characterized as skimianine based on 1H-NMR spectroscopy and comparison with spectra of authentic samples. Skimmianine did not have antimicrobial activity in this test system. W. ugandensis water extracts elicited antibacterial activity against both Escherischia coli and Staphylococcus aureus in the agar well assay but not in the disc diffusion assay. Warburgia ugandensis water extracts and fraction 27-163D also showed antifungal activity against Candida albicans. Chromatography of extracts of Warburgia ugandensis stem bark afforded compound 49–169K, which was characterized as the sesquiterpine muzigadial (by 1H-NMR spectroscopy), which did not show antibacterial activity but had antifungal activity against C. albicans. Therefore, the claimed efficacy of W. ugandensis could be attributable to antibacterial and antifungal activity of its components. Since Z. chalybeum extracts had neither antifungal nor antibacterial activities, its mode of action is unclear from these results.
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antibacterial and antifungal activities of extracts of zanthoxylum chalybeum and warburgia ugandensis ugandan medicinal plants
African Health Sciences, 2001Co-Authors: Deogracious Olila, J OpudaasiboAbstract:Measles is a killer disease of children in Uganda. The treatment of the disease is mainly directed at the secondary microbial infections. A large proportion of the population in Uganda still relies on the use of herbal remedies, which have been claimed to produce beneficial responses. In this study, the efficacy of Warburgia ugandensis and Zanthoxylum chalybeum against common bacteria and fungi was investigated. Bactericidal and antifungal assays were done using extracts derived from Z. chalybeum and W. ugandensis (agar well diffusion, disc diffusion and colony count assays). All extracts (ethanolic, petroleum ether and aqueous) of Z. chalybeum did not show antimicrobial activity. Phytochemical investigations of Zanthoxylum chalybeum (seed) yielded a pure crystalline alkaloid (27–135D) which was characterized as skimianine based on 1H-NMR spectroscopy and comparison with spectra of authentic samples. Skimmianine did not have antimicrobial activity in this test system. W. ugandensis water extracts elicited antibacterial activity against both Escherischia coli and Staphylococcus aureus in the agar well assay but not in the disc diffusion assay. Warburgia ugandensis water extracts and fraction 27-163D also showed antifungal activity against Candida albicans. Chromatography of extracts of Warburgia ugandensis stem bark afforded compound 49–169K, which was characterized as the sesquiterpine muzigadial (by 1H-NMR spectroscopy), which did not show antibacterial activity but had antifungal activity against C. albicans. Therefore, the claimed efficacy of W. ugandensis could be attributable to antibacterial and antifungal activity of its components. Since Z. chalybeum extracts had neither antifungal nor antibacterial activities, its mode of action is unclear from these results.
Jose Rubens Pirani - One of the best experts on this subject based on the ideXlab platform.
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alkaloids from stems of esenbeckia leiocarpa engl rutaceae as potential treatment for alzheimer disease
Molecules, 2010Co-Authors: Elaine Monteiro Cardosolopes, Jose Rubens Pirani, James Andreas Maier, Marcelo Rogerio Da Silva, Luis Octavio Regasini, Simone Yasue Simote, Norberto Peporine Lopes, Vanderlan Da Silva Bolzani, Maria Claudia Marx YoungAbstract:Esenbeckia leiocarpa Engl. (Rutaceae), popularly known as guaranta, goiabeira, is a native tree from Brazil. Bioactivity-guided fractionation of the ethanol stems extract afforded the isolation of six alkaloids: leiokinine A, leptomerine, kokusaginine, Skimmianine, maculine and flindersiamine. All isolated compounds were tested for acetyl cholinesterase inhibition, in vitro and displayed anticholinesterasic activity. The alkaloid leptomerine showed the highest activity (IC50 = 2.5 μM), similar to that of the reference compound galanthamine (IC50 = 1.7 μM). The results showed for the first time the presence of alkaloids leptomerine and Skimmianine in E. leiocarpa (Engl.) with potent anticholinesterasic activity.
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activities of extracts and compounds from spiranthera odoratissima st hil rutaceae in leaf cutting ants and their symbiotic fungus
Journal of the Brazilian Chemical Society, 2010Co-Authors: Ana Paula Terezan, Paulo C Vieira, Joao B Fernandes, Fatima Das M G F Da Silva, Raquel Andrade Rossi, Roberta N A Almeida, Tais Garcia Freitas, Odair Correa Bueno, Fernando Carlos Pagnocca, Jose Rubens PiraniAbstract:O estudo dos extratos dos galhos da planta Spiranthera odoratissima St. Hil. (Rutaceae) levou ao isolamento de alcaloides furoquinolinicos (dictamina, g-fagarina e esquimianina) e 2-arilquinolin4-ona (2-fenil-1-metilquinolin-4-ona) e limonoides (acido limonexico e limonina). Os compostos 2-fenil-1-metilquinolin-4-ona e acido limonexico foram isolados pela primeira vez no genero Spiranthera. Os alcaloides furoquinolinicos, 2-arilquinolin-4-ona e os limonoides mostraram atividade inseticida e/ou fungicida no ninho da formiga Atta sexdens rubropilosa. The study of the Spiranthera odoratissima St. Hil (Rutaceae) branches extracts led to the isolation of the furoquinoline (dictamine, g-fagarine and Skimmianine) and 2-arylquinoli-4-one (1-methyl-2-phenylquinolin-4-one) alkaloids and limonoids (limonexic acid and limonin). The compounds 1-methyl-2-phenylquinolin-4-one and limonexic acid were isolated for the first time from the Spiranthera. These furoquinoline and 2-arylquinoli-4-one alkaloids and limonoids showed insecticidal and/or fungicidal activity in the nest of the Atta sexdens rubropilosa.
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Cumarinas e alcaloides de Rauia resinosa (rutaceae) Coumarins and alkaloids from Rauia resinosa (rutaceae)
Sociedade Brasileira de Química, 2010Co-Authors: Tatiane Regina Albarici, João Batista Fernandes, Maria Fátima Das Graças Fernandes Da ,silva, Paulo Cezar Vieira, Jose Rubens PiraniAbstract:The genus Rauia, that is poorly chemically studied, belongs to the Rutaceae family. This family has been known to contain a large variety of secondary metabolites. Our phytochemical investigation of the stem and leaves of Rauia resinosa has led to the identification of the structurally related coumarins: murralongin (1), murrangatin (2), munomicrolin (3), murrangatin diacetate (4), umbelliferone (5), rauianin (6) and one novel coumarin: 3-ethylrauianin (7); the alkaloids: N-methyl-4-methoxy-2-quinolone (8), mirtopsine (9), dictamine (10), γ-fagarine (11), Skimmianine (12), Z-dimethylrhoifolinate (13), zantodioline (14), zantobungeanine (15), veprissine (16), one novel alkaloid 7-hydroxy-8-methoxy-N-methylflindersine (17) and 8-hydroxy-N-methylflindersine (18) that is described as a natural product for the first time, and a mixture of steroids: as sitosterol and stigmasterol
Vieira,paulo C. - One of the best experts on this subject based on the ideXlab platform.
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Enzymatic inhibition studies of selected flavonoids and chemosystematic significance of polymethoxylated flavonoids and quinoline alkaloids in Neoraputia (Rutaceae)
'FapUNIFESP (SciELO)', 2015Co-Authors: Moraes, Valéria R. De S., Fernandes,joão B., Vieira,paulo C., Fatima Das M G F Da Silva, Tomazela, Daniela M., Ferracin, Ricardo J., Garcia, Cleverson F., Sannomiya Míriam, Soriano M. Del Pilar C., Rodrigues Filho EdsonAbstract:Our taxonomic interest in the Neoraputia stimulated an investigation of N. paraensis searching for alkaloids. Fractions were monitored by ¹H NMR and ESI-MS/MS and only those which showed features of anthranilate alkaloids and flavonoids absent in the previous investigations were examined. Stems afforded the alkaloids flindersine, Skimmianine, 8-methoxyflindersine and dictamnine; leaves yielded 3',4',7,8-tetramethoxy-5,6-(2,2-dimethylpyrano)-flavone, 3',4',5,7,8-pentamethoxyflavone, 5-hydroxy-3',4',6,7-tetramethoxyflavone, 3',4'-methylenedioxy-5,6,7-trimethoxyflavone and 5-hydroxy-3',4'-methylenedioxy-6,7-dimethoxyflavone. The alkaloids have remained undiscovered for 10 years. A number of flavonoids isolated from N. paraensis, N. magnifica, Murraya paniculata, Citrus sinensis graft (Rutaceae), Lonchocarpus montanus (Leguminosae) were evaluated for their ability to inhibit the enzymatic activity of the protein glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. Highly oxygenated flavones and isoflavone were the most actives.Nosso interesse quimiotaxonômico sobre Neoraputia nos estimulou a examinar N. paraensis, visando a busca de alcalóides. As frações foram monitoradas via RMN ¹H e ESI-EM/EM e foram analisadas somente aquelas cujos espectros apresentavam características de alcalóides do ácido antranílico e flavonóides não isolados anteriormente. Foram isolados do caule os alcalóides flindersina, skimmianina, 8-metoxiflindersina e dictamnina; das folhas os flavonóides 3',4',7,8-tetrametoxi-5,6-(2,2-dimetilpirano)-flavona, 3',4',5,7,8-pentametoxiflavona, 5-hidroxi-3',4',6,7-tetrametoxiflavona, 3',4'-metilenodioxi-5,6,7-trimetoxiflavona e 5-hidroxi-3',4'-metilenodioxi-6,7-dimetoxiflavona,. Os alcalóides do ácido antranílico não foram encontrados em dez anos. Vários flavonóides isolados de N. paraensis, N. magnifica, Murraya paniculata, enxerto de Citrus sinensis (Rutaceae) e Lonchocarpus montanus (Leguminosae) foram testados frente a gliceraldeído-3-fosfato desidrogenase de Trypanosoma cruzi, visando verificar seus potenciais em inibir a atividade da enzima. Os flavonóides polimetoxilados e um isoflavonóide foram os mais ativos.380387Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
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Enzymatic inhibition studies of selected flavonoids and chemosystematic significance of polymethoxylated flavonoids and quinoline alkaloids in Neoraputia (Rutaceae)
Sociedade Brasileira de Química, 2015Co-Authors: Moraes, Valéria R. De S., Fernandes,joão B., Vieira,paulo C., Fatima Das M G F Da Silva, Tomazela, Daniela M., Ferracin, Ricardo J., Garcia, Cleverson F., Sannomiya Míriam, Soriano M. Del Pilar C., Rodrigues Filho EdsonAbstract:Our taxonomic interest in the Neoraputia stimulated an investigation of N. paraensis searching for alkaloids. Fractions were monitored by ¹H NMR and ESI-MS/MS and only those which showed features of anthranilate alkaloids and flavonoids absent in the previous investigations were examined. Stems afforded the alkaloids flindersine, Skimmianine, 8-methoxyflindersine and dictamnine; leaves yielded 3',4',7,8-tetramethoxy-5,6-(2,2-dimethylpyrano)-flavone, 3',4',5,7,8-pentamethoxyflavone, 5-hydroxy-3',4',6,7-tetramethoxyflavone, 3',4'-methylenedioxy-5,6,7-trimethoxyflavone and 5-hydroxy-3',4'-methylenedioxy-6,7-dimethoxyflavone. The alkaloids have remained undiscovered for 10 years. A number of flavonoids isolated from N. paraensis, N. magnifica, Murraya paniculata, Citrus sinensis graft (Rutaceae), Lonchocarpus montanus (Leguminosae) were evaluated for their ability to inhibit the enzymatic activity of the protein glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. Highly oxygenated flavones and isoflavone were the most actives.Nosso interesse quimiotaxonômico sobre Neoraputia nos estimulou a examinar N. paraensis, visando a busca de alcalóides. As frações foram monitoradas via RMN ¹H e ESI-EM/EM e foram analisadas somente aquelas cujos espectros apresentavam características de alcalóides do ácido antranílico e flavonóides não isolados anteriormente. Foram isolados do caule os alcalóides flindersina, skimmianina, 8-metoxiflindersina e dictamnina; das folhas os flavonóides 3',4',7,8-tetrametoxi-5,6-(2,2-dimetilpirano)-flavona, 3',4',5,7,8-pentametoxiflavona, 5-hidroxi-3',4',6,7-tetrametoxiflavona, 3',4'-metilenodioxi-5,6,7-trimetoxiflavona e 5-hidroxi-3',4'-metilenodioxi-6,7-dimetoxiflavona,. Os alcalóides do ácido antranílico não foram encontrados em dez anos. Vários flavonóides isolados de N. paraensis, N. magnifica, Murraya paniculata, enxerto de Citrus sinensis (Rutaceae) e Lonchocarpus montanus (Leguminosae) foram testados frente a gliceraldeído-3-fosfato desidrogenase de Trypanosoma cruzi, visando verificar seus potenciais em inibir a atividade da enzima. Os flavonóides polimetoxilados e um isoflavonóide foram os mais ativos
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Prenylindole alkaloids from Raputia praetermissa (Rutaceae) and their chemosystematic significance
Sociedade Brasileira de Química, 2011Co-Authors: Veiga,thiago André M, Fernandes,joão B., Vieira,paulo C., Fatima Das M G F Da SilvaAbstract:The dichloromethane extract from the stems of Raputia praetermissa afforded four new compounds, 4-deoxyraputindole C (1), raputimonoindoleA-B (2, 3), and hexadecanyl 2-hydroxy-4-methoxy-cinnamate (5), besides the alkaloids 5-(4-methoxymethylfuran-2-yl)-1H-indole (raputimonoindole C), furoquinolines maculosidine, robustine, evolitrine and dictamnine. The hexane extract yielded N-methyl-4-methoxyquinolin-2(1H)-one, Skimmianine, cycloartenone, sitosterol, stigmasterol and sitostenone. The anthranilate alkaloid content indicates that the genus is strongly related to those included in Cusparieae tribe, but differs from Neoraputia by the absence of prenylindole alkaloids in the late, whose species have previously been placed in Raputia
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Activities of extracts and compounds from Spiranthera odoratissima St. Hil. (Rutaceae) in leaf-cutting ants and their symbiotic fungus
Sociedade Brasileira de Química, 2010Co-Authors: Terezan,ana Paula, Rossi,raquel Andrade, Almeida,roberta N. A., Freitas,taís Garcia, Fernandes,joão B., Fátima Das Graças Fernandes M. Da ,silva, Vieira,paulo C., Bueno,odair C., Pagnocca,fernando C., Pirani,josé R.Abstract:The study of the Spiranthera odoratissima St. Hil (Rutaceae) branches extracts led to the isolation of the furoquinoline (dictamine, γ-fagarine and Skimmianine) and 2-arylquinoli-4-one (1-methyl-2-phenylquinolin-4-one) alkaloids and limonoids (limonexic acid and limonin). The compounds 1-methyl-2-phenylquinolin-4-one and limonexic acid were isolated for the first time from the Spiranthera. These furoquinoline and 2-arylquinoli-4-one alkaloids and limonoids showed insecticidal and/or fungicidal activity in the nest of the Atta sexdens rubropilosa
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Activities of Extracts and Compounds from Spiranthera odoratissima St. Hil. (Rutaceae) in Leaf-cutting Ants and their Symbiotic Fungus
Soc Brasileira Quimica, 2010Co-Authors: Terezan,ana Paula, Rossi,raquel Andrade, Almeida,roberta N. A., Freitas,taís Garcia, Fernandes,joão B., Vieira,paulo C., Bueno,odair C., Gracas Fernandes Da Silva, M. Fatima Das, Pagnocca, Fernando Carlos, Pirani,josé R.Abstract:The study of the Spiranthera odoratissima St. Hil (Rutaceae) branches extracts led to the isolation of the furoquinoline (dictamine, gamma-fagarine and Skimmianine) and 2-arylquinoli-4-one (1-methyl-2-phenylquinolin-4-one) alkaloids and limonoids (limonexic acid and limonin). The compounds 1-methyl-2-phenylquinolin-4-one and limonexic acid were isolated for the first time from the Spiranthera. These furoquinoline and 2-arylquinoli-4-one alkaloids and limonoids showed insecticidal and/or fungicidal activity in the nest of the Atta sexdens rubropilosa.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
Deogracious Olila - One of the best experts on this subject based on the ideXlab platform.
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screening of extracts of zanthoxylum chalybeum and warburgia ugandensis for activity against measles virus swartz and edmonston strains in vitro
African Health Sciences, 2002Co-Authors: Deogracious Olila, J OpudaasiboAbstract:A large proportion of the population in Uganda still relies on the use of plant extracts for treatment of various ailments. This study tested the claimed efficacy of some plants in the treatment of measles. In vitro antiviral assays were performed on extracts of two medicinal plants (Warburgia ugandensis and Zanthoxylum chalybeum) using measles virus (Edmonston and Swartz strains) as the test organisms. The assays performed were the neutralisation tests and the plaque reduction assays. Of the two plants Z. chalybeum had demonstrable in vitro antiviral activity in the seed extracts (titer reduction factor [TRF]: 100, for the ethanolic extract). The in vitro antiviral activity of the seed extracts was demonstrated to be due to compound 27–135D (TRF=1000), which was characterized by 1H-NMR spectroscopy as the alkaloid Skimmianine. Skimmianine had minimal toxicity to VERO cell lines. The petroleum ether extracts and the ethanolic extracts of Warburgia ugandensis had no inhibitory effect on cytopathic effect (CPE) formation, especially at the maximal non-toxic dose (MNTD). The extracts of W. ugandensis were highy toxic to VERO cell lines. The TRF values for the stem bark extracts of W. ugandensis were: water extract, 10; ethanolic extract, 1; fraction 27–163D, 100., which were regarded to be too low. Seed extracts of Z.chalybeum therefore probably cure measles due to the antiviral effect of Skimmianine. It is not clear how extracts of W. ugandensis produce a beneficial response in measles disease, if at all.
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screening of extracts of zanthoxylum chalybeum and warburgia ugandensis for activity against measles virus swartz and edmonston strains in vitro
African Health Sciences, 2002Co-Authors: Deogracious Olila, J OpudaasiboAbstract:A large proportion of the population in Uganda still relies on the use of plant extracts for treatment of various ailments. This study tested the claimed efficacy of some plants in the treatment of measles. In vitro antiviral assays were performed on extracts of two medicinal plants (Warburgia ugandensis and Zanthoxylum chalybeum) using measles virus (Edmonston and Swartz strains) as the test organisms. The assays performed were the neutralisation tests and the plaque reduction assays. Of the two plants Z. chalybeum had demonstrable in vitro antiviral activity in the seed extracts (titer reduction factor [TRF]: 100, for the ethanolic extract). The in vitro antiviral activity of the seed extracts was demonstrated to be due to compound 27–135D (TRF=1000), which was characterized by 1H-NMR spectroscopy as the alkaloid Skimmianine. Skimmianine had minimal toxicity to VERO cell lines. The petroleum ether extracts and the ethanolic extracts of Warburgia ugandensis had no inhibitory effect on cytopathic effect (CPE) formation, especially at the maximal non-toxic dose (MNTD). The extracts of W. ugandensis were highy toxic to VERO cell lines. The TRF values for the stem bark extracts of W. ugandensis were: water extract, 10; ethanolic extract, 1; fraction 27–163D, 100., which were regarded to be too low. Seed extracts of Z.chalybeum therefore probably cure measles due to the antiviral effect of Skimmianine. It is not clear how extracts of W. ugandensis produce a beneficial response in measles disease, if at all.
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antibacterial and antifungal activities of extracts of zanthoxylum chalybeum and warburgia ugandensis ugandan medicinal plants
African Health Sciences, 2001Co-Authors: Deogracious Olila, J OpudaasiboAbstract:Measles is a killer disease of children in Uganda. The treatment of the disease is mainly directed at the secondary microbial infections. A large proportion of the population in Uganda still relies on the use of herbal remedies, which have been claimed to produce beneficial responses. In this study, the efficacy of Warburgia ugandensis and Zanthoxylum chalybeum against common bacteria and fungi was investigated. Bactericidal and antifungal assays were done using extracts derived from Z. chalybeum and W. ugandensis (agar well diffusion, disc diffusion and colony count assays). All extracts (ethanolic, petroleum ether and aqueous) of Z. chalybeum did not show antimicrobial activity. Phytochemical investigations of Zanthoxylum chalybeum (seed) yielded a pure crystalline alkaloid (27–135D) which was characterized as skimianine based on 1H-NMR spectroscopy and comparison with spectra of authentic samples. Skimmianine did not have antimicrobial activity in this test system. W. ugandensis water extracts elicited antibacterial activity against both Escherischia coli and Staphylococcus aureus in the agar well assay but not in the disc diffusion assay. Warburgia ugandensis water extracts and fraction 27-163D also showed antifungal activity against Candida albicans. Chromatography of extracts of Warburgia ugandensis stem bark afforded compound 49–169K, which was characterized as the sesquiterpine muzigadial (by 1H-NMR spectroscopy), which did not show antibacterial activity but had antifungal activity against C. albicans. Therefore, the claimed efficacy of W. ugandensis could be attributable to antibacterial and antifungal activity of its components. Since Z. chalybeum extracts had neither antifungal nor antibacterial activities, its mode of action is unclear from these results.
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antibacterial and antifungal activities of extracts of zanthoxylum chalybeum and warburgia ugandensis ugandan medicinal plants
African Health Sciences, 2001Co-Authors: Deogracious Olila, J OpudaasiboAbstract:Measles is a killer disease of children in Uganda. The treatment of the disease is mainly directed at the secondary microbial infections. A large proportion of the population in Uganda still relies on the use of herbal remedies, which have been claimed to produce beneficial responses. In this study, the efficacy of Warburgia ugandensis and Zanthoxylum chalybeum against common bacteria and fungi was investigated. Bactericidal and antifungal assays were done using extracts derived from Z. chalybeum and W. ugandensis (agar well diffusion, disc diffusion and colony count assays). All extracts (ethanolic, petroleum ether and aqueous) of Z. chalybeum did not show antimicrobial activity. Phytochemical investigations of Zanthoxylum chalybeum (seed) yielded a pure crystalline alkaloid (27–135D) which was characterized as skimianine based on 1H-NMR spectroscopy and comparison with spectra of authentic samples. Skimmianine did not have antimicrobial activity in this test system. W. ugandensis water extracts elicited antibacterial activity against both Escherischia coli and Staphylococcus aureus in the agar well assay but not in the disc diffusion assay. Warburgia ugandensis water extracts and fraction 27-163D also showed antifungal activity against Candida albicans. Chromatography of extracts of Warburgia ugandensis stem bark afforded compound 49–169K, which was characterized as the sesquiterpine muzigadial (by 1H-NMR spectroscopy), which did not show antibacterial activity but had antifungal activity against C. albicans. Therefore, the claimed efficacy of W. ugandensis could be attributable to antibacterial and antifungal activity of its components. Since Z. chalybeum extracts had neither antifungal nor antibacterial activities, its mode of action is unclear from these results.
Maria Claudia Marx Young - One of the best experts on this subject based on the ideXlab platform.
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alkaloids from stems of esenbeckia leiocarpa engl rutaceae as potential treatment for alzheimer disease
Molecules, 2010Co-Authors: Elaine Monteiro Cardosolopes, Jose Rubens Pirani, James Andreas Maier, Marcelo Rogerio Da Silva, Luis Octavio Regasini, Simone Yasue Simote, Norberto Peporine Lopes, Vanderlan Da Silva Bolzani, Maria Claudia Marx YoungAbstract:Esenbeckia leiocarpa Engl. (Rutaceae), popularly known as guaranta, goiabeira, is a native tree from Brazil. Bioactivity-guided fractionation of the ethanol stems extract afforded the isolation of six alkaloids: leiokinine A, leptomerine, kokusaginine, Skimmianine, maculine and flindersiamine. All isolated compounds were tested for acetyl cholinesterase inhibition, in vitro and displayed anticholinesterasic activity. The alkaloid leptomerine showed the highest activity (IC50 = 2.5 μM), similar to that of the reference compound galanthamine (IC50 = 1.7 μM). The results showed for the first time the presence of alkaloids leptomerine and Skimmianine in E. leiocarpa (Engl.) with potent anticholinesterasic activity.
