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Joachim W. Fluhr - One of the best experts on this subject based on the ideXlab platform.
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Acute irritant threshold correlates with barrier function, Skin hydration and contact hypersensitivity in atopic dermatitis and rosacea.
Experimental dermatology, 2013Co-Authors: Razvigor Darlenski, Jana Kazandjieva, Nikolai Tsankov, Joachim W. FluhrAbstract:The aim of the study was to disclose interactions between epidermal barrier, Skin irritation and sensitization in healthy and diseased Skin. Transepidermal water loss (TEWL) and stratum corneum hydration (SCH) were assessed in adult patients with atopic dermatitis (AD), rosacea and healthy controls. A 4-h patch test with seven concentrations of sodium lauryl sulphate was performed to determine the irritant threshold (IT). Contact sensitization pattern was revealed by patch testing with European baseline series. Subjects with a lower IT had higher TEWL values and lower SCH. Subjects with positive allergic reactions had significantly lower IT. In AD, epidermal barrier deterioration was detected on both volar forearm and nasolabial fold, while in rosacea, impeded Skin Physiology parameters were observed on the facial Skin only, suggesting that barrier impediment is restricted to the face in rosacea, in contrast with AD where the abnormal Skin Physiology is generalized.
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In vivo Skin treatment with tissue-tolerable plasma influences Skin Physiology and antioxidant profile in human stratum corneum
Experimental Dermatology, 2012Co-Authors: Joachim W. Fluhr, Olaf Lademann, Sven Sassning, Wolfram Sterry, Axel Kramer, Heike Richter, Maxim E. Darvin, Sabine Schanzer, Jürgen LademannAbstract:The antimicrobial treatment of wounds is still a major problem. Tissue-tolerable electrical plasma (TTP) is a new approach for topical microbial disinfection of the Skin surface. The aim of the present study was to investigate the influence of TTP on a carotenoid profile in relation to Skin Physiology parameters (epidermal barrier function, stratum corneum (SC) hydration, surface temperature and irritation parameters). We were interested in the interaction of TTP and the antioxidative network, as well as the consequences for Skin Physiology parameters. These parameters are also indicative of TTP safety in vivo. For plasma application, 'Kinpen 09' was used (surface exposure 30-43°C) for 3 s. Beta-carotene and water profiles were assessed by in vivo Raman microspectroscopy (Skin composition analyzer 3510). Skin Physiology parameters were measured with Tewameter TM 300, Corneometer CM 825, Skin thermometer and Chromameter CR 300. All parameters were assessed non-invasively on seven healthy volunteers before and after plasma application in vivo. We could show that TTP application leads to a decrease in beta-carotene especially in the superficial SC. Skin-surface temperature increased by 1.74°C, while the transepidermal water loss (TEWL) increase indicated an impaired barrier function. SC hydration decreased as seen in water profile especially in the superficial layers and capacitance values. A slight increase in Skin redness was measurable. The induction of reactive oxygen species is probably the major contributor of TTP efficacy in Skin disinfection. Skin Physiology parameters were influenced without damaging the Skin or Skin functions, indicating the safety of TTP under in vivo conditions.
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Functional Skin adaptation in infancy - almost complete but not fully competent.
Experimental dermatology, 2010Co-Authors: Joachim W. Fluhr, Razvigor Darlenski, Philippe Msika, C De Belilovsky, Jean Pierre Hachem, Alain Taïeb, Caroline Baudouin, Enzo BerardescaAbstract:Early postnatal life is a period of active functional reorganization and cutaneous physiological adaptation to the extrauterine environment. Skin as the outermost organ of mammalians is endowed of multiple functions such as protection, secretion, absorption and thermoregulation. Birth stimulates the epidermal barrier maturation and the Skin surface acidification especially in premature infants. In full-term infants the developed stratum corneum accomplishes competent barrier function, in contrast to prematures. Complete barrier maturation in preterm infants is fulfilled by 2-4 weeks of the postnatal life. However, in preterms with 23-25 weeks gestational age this process takes longer. Versatile regulatory mechanisms, namely Skin surface acidity, calcium ion gradient and nuclear hormone receptors/ligands are interrelated in the complex postnatal newborn adaptation. The Skin of newborns is adjusting quickly to the challenging environmental conditions of the postpartum. However, certain functions, for example, microcirculation, continue to develop even beyond the neonatal period, that is, up to the age of 14-17 weeks. Different environmental factors (for instance, dry and cold climate, diapers and cosmetic care procedures) influence the postnatal development of Skin functional parameters such as stratum corneum hydration and the permeability barrier especially in premature infants. The aim of this article is to summarize the current knowledge on Skin Physiology in newborn and infants with a practical approach and to discuss the possible clinical consequences. This review offers the readership a critical and practical overview of Skin Physiology in newborns and infants. It emphasizes possible new research fields in neonatal and infantile Skin Physiology.
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Comprar Practical Aspects Of Cosmetic Testing. How To Set Up A Scientific Study In Skin Physiology | Joachim W. Fluhr | 9783642050664 | Springer
2010Co-Authors: Joachim W. FluhrAbstract:Tienda online donde Comprar Practical Aspects Of Cosmetic Testing. How To Set Up A Scientific Study In Skin Physiology al precio 151,19 € de Joachim W. Fluhr, tienda de Libros de Medicina, Libros de Cirugia - Cirugia plastica y reparadora
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Silver-loaded seaweed-based cellulosic fiber improves epidermal Skin Physiology in atopic dermatitis: safety assessment, mode of action and controlled, randomized single-blinded exploratory in vivo study.
Experimental dermatology, 2009Co-Authors: Joachim W. Fluhr, Maria Breternitz, Doreen Kowatzki, Andrea Bauer, Joerg Bossert, Peter Elsner, Uta-christina HiplerAbstract:Please cite this paper as: Silver-loaded seaweed-based cellulosic fibre improves epidermal Skin Physiology in atopic dermatitis: safety assessment, mode of action and controlled, randomized single-blinded exploratory in vivo study. Experimental Dermatology 2010; 19: e9–e15. Abstract Background: The epidermal part of the Skin is the major interface between the internal body and the external environment. The Skin has a specific Physiology and is to different degrees adapted for protection against multiple exogenous stress factors. Clothing is the material with the longest and most intensive contact to human Skin. It plays a critical role especially in inflammatory dermatoses or Skin conditions with an increased susceptibility of bacterial and fungal infections like atopic dermatitis. Previously, we have shown a dose-dependent antibacterial and antifungal activity of silver-loaded seaweed-based cellulosic fibres. Aim of the study: We studied the mode of action of silver-loaded seaweed-based cellulosic fiber and performed a broad safety assessment. The principal aim was to analyse the effects of wearing the textile on epidermal Skin Physiology in 37 patients with atopic dermatitis in a controlled, randomized single-blinded in vivo study. Furthermore, the sensitization potential was tested in a patch test in 111 panellists. Results: We could demonstrate in vitro a dose-dependent scavenging of induced reactive oxygen species by silver-loaded seaweed-based cellulosic fibers. Safety assessment of these fibres showed no detectable release of silver ions. Furthermore, ex vivo assessment after 24 h application both in healthy volunteers and patients with atopic dermatitis by sequential tape stripping and subsequently raster electron microscopy and energy dispersive microanalysis analysis revealed no detectable amounts of silver in any of stratum corneum layers. Serum analysis of silver showed no detectable levels. The in vivo patch testing of 111 volunteers revealed no sensitization against different SeaCell® Active (SeaCell GmbH, Rudolstadt, Germany) containing fabrics. The in vivo study on 37 patients with known atopic dermatitis and mild-to-moderate eczema on their arms were randomly assigned to either silver-loaded seaweed fibre T-shirts or to cotton T-shirts for 8 weeks. A significant reduction in Staphylococcus aureus colonization was detectable for the silver T-shirts compared with cotton T-shirts without any changes in non-pathogenic surface bacteria colonization. Furthermore, a more pronounced improvement in barrier function (transepidermal water loss) was observed in mildly involved eczema areas during the first 4 weeks of the study. Stratum corneum hydration and surface pH improved in both treatment groups over time. Conclusion: The tested silver-loaded seaweed fibre can be regarded as safe and seams to be suited for application in bio-active textiles in atopic dermatitis based on its positive in vivo activity.
Jürgen Lademann - One of the best experts on this subject based on the ideXlab platform.
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In vivo Skin treatment with tissue-tolerable plasma influences Skin Physiology and antioxidant profile in human stratum corneum
Experimental Dermatology, 2012Co-Authors: Joachim W. Fluhr, Olaf Lademann, Sven Sassning, Wolfram Sterry, Axel Kramer, Heike Richter, Maxim E. Darvin, Sabine Schanzer, Jürgen LademannAbstract:The antimicrobial treatment of wounds is still a major problem. Tissue-tolerable electrical plasma (TTP) is a new approach for topical microbial disinfection of the Skin surface. The aim of the present study was to investigate the influence of TTP on a carotenoid profile in relation to Skin Physiology parameters (epidermal barrier function, stratum corneum (SC) hydration, surface temperature and irritation parameters). We were interested in the interaction of TTP and the antioxidative network, as well as the consequences for Skin Physiology parameters. These parameters are also indicative of TTP safety in vivo. For plasma application, 'Kinpen 09' was used (surface exposure 30-43°C) for 3 s. Beta-carotene and water profiles were assessed by in vivo Raman microspectroscopy (Skin composition analyzer 3510). Skin Physiology parameters were measured with Tewameter TM 300, Corneometer CM 825, Skin thermometer and Chromameter CR 300. All parameters were assessed non-invasively on seven healthy volunteers before and after plasma application in vivo. We could show that TTP application leads to a decrease in beta-carotene especially in the superficial SC. Skin-surface temperature increased by 1.74°C, while the transepidermal water loss (TEWL) increase indicated an impaired barrier function. SC hydration decreased as seen in water profile especially in the superficial layers and capacitance values. A slight increase in Skin redness was measurable. The induction of reactive oxygen species is probably the major contributor of TTP efficacy in Skin disinfection. Skin Physiology parameters were influenced without damaging the Skin or Skin functions, indicating the safety of TTP under in vivo conditions.
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Application of optical non-invasive methods in Skin Physiology
Laser Physics Letters, 2008Co-Authors: Jürgen Lademann, Wolfram Sterry, Alexa Patzelt, Christina Antoniou, Heike Richter, Maxim E. Darvin, S. KochAbstract:In the present paper the application of optical non-invasive methods in dermatology and cosmetology is discussed. Laser scanning microscopy (LSM) and optical coherent tomography (OCT) are the most promising methods for this application. Using these methods, the analysis of different Skin parameters like dryness and oiliness of the Skin, the barrier function and the structure of furrows and wrinkles are discussed. Additionally the homogeneity of distribution of topically applied creams, as well as their penetration into the Skin were investigated. It is shown that these methods are highly valuable in dermatology for diagnostic and therapy control and for basic research, for instance in the field of structure analysis of hair follicles and sweat glands. The vertical images of the tissue produced by OCT can be easily compared with histological sections. Unfortunately, the resolution of the OCT technique is not high enough to carry out measurements on a cellular level, as is possible by LSM. LSM has the advantage that it can be used for the investigation of penetration and storage processes of topically applied substances, if these substances have fluorescent properties or if they are fluorescent-labelled.
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Gender-Related Differences in the Physiology of the Stratum Corneum
Dermatology (Basel Switzerland), 2005Co-Authors: Ute Jacobi, Wolfram Sterry, Julien Gautier, Jürgen LademannAbstract:Background: Only few studies on gender-related differences in Skin Physiology have been performed – and they provided conflicting results. Objective: In the prese
Bruce J. Nicholson - One of the best experts on this subject based on the ideXlab platform.
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the role of connexins in ear and Skin Physiology functional insights from disease associated mutations
Biochimica et Biophysica Acta, 2013Co-Authors: Ji Xu, Bruce J. NicholsonAbstract:Abstract Defects in several different connexins have been associated with several different diseases. The most common of these is deafness, where a few mutations in connexin (Cx) 26 have been found to contribute to over 50% of the incidence of non-syndromic deafness in different human populations. Other mutations in Cx26 or Cx30 have also been associated with various Skin phenotypes linked to deafness (palmoplanta keratoderma, Bart–Pumphrey syndrome, Vohwinkel syndrome, keratitis–ichthyosis–deafness syndrome, etc.). The large array of disease mutants offers unique opportunities to gain insights into the underlying function of gap junction proteins and their channels in the normal and pathogenic physiologies of the cochlea and epidermis. This review focuses on those mutants where the impact on channel function has been assessed, and correlated with the disease phenotype, or organ function in knock-out mouse models. These approaches have provided evidence supporting a role of gap junctions and hemichannels in K+ removal and recycling in the ear, as well as possible roles for nutrient passage, in the cochlea. In contrast, increases in hemichannel opening leading to increased cell death, were associated with several keratitis–ichthyosis–deafness syndrome Skin disease/hearing mutants. In addition to providing clues for therapeutic strategies, these findings allow us to better understand the specific functions of connexin channels that are important for normal tissue function. This article is part of a Special Issue entitled: The communicating junctions, roles and dysfunctions.
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The role of connexins in ear and Skin Physiology — Functional insights from disease-associated mutations
Biochimica et Biophysica Acta, 2012Co-Authors: Ji Xu, Bruce J. NicholsonAbstract:Abstract Defects in several different connexins have been associated with several different diseases. The most common of these is deafness, where a few mutations in connexin (Cx) 26 have been found to contribute to over 50% of the incidence of non-syndromic deafness in different human populations. Other mutations in Cx26 or Cx30 have also been associated with various Skin phenotypes linked to deafness (palmoplanta keratoderma, Bart–Pumphrey syndrome, Vohwinkel syndrome, keratitis–ichthyosis–deafness syndrome, etc.). The large array of disease mutants offers unique opportunities to gain insights into the underlying function of gap junction proteins and their channels in the normal and pathogenic physiologies of the cochlea and epidermis. This review focuses on those mutants where the impact on channel function has been assessed, and correlated with the disease phenotype, or organ function in knock-out mouse models. These approaches have provided evidence supporting a role of gap junctions and hemichannels in K+ removal and recycling in the ear, as well as possible roles for nutrient passage, in the cochlea. In contrast, increases in hemichannel opening leading to increased cell death, were associated with several keratitis–ichthyosis–deafness syndrome Skin disease/hearing mutants. In addition to providing clues for therapeutic strategies, these findings allow us to better understand the specific functions of connexin channels that are important for normal tissue function. This article is part of a Special Issue entitled: The communicating junctions, roles and dysfunctions.
Joachim W Fluh - One of the best experts on this subject based on the ideXlab platform.
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infant epidermal Skin Physiology adaptation after birth
British Journal of Dermatology, 2012Co-Authors: Joachim W Fluh, Nadege Lachma, Caroline Audoui, Philippe Msika, C De Belilovsky, Razvigo Darlenski, Jean Pierre HachemAbstract:Summary Background Functional and structural Skin adaptation is a dynamic process which starts immediately after birth in humans and in mammalian Skin in general. This adjustment to the extrauterine dry environment is accomplished in the first year of postnatal life of humans. Objectives To assess the dynamic changes in vivo after birth in the molecular composition and Skin Physiology parameters compared with older children and adults. Methods The molecular composition of the stratum corneum (SC) and the water profile were investigated noninvasively by in vivo Raman confocal microscopy as a function of depth. Functional parameters including transepidermal water loss (characterizing epidermal permeability barrier), capacitance (as an indirect parameter for SC hydration) and Skin surface pH were assessed noninvasively. The measurements were performed in 108 subjects divided into six age groups: full-term newborns (1–15 days), babies aged 5–6 weeks, babies aged 6 ± 1 months, children aged 1–2 years, children aged 4–5 years and adults aged 20–35 years. Results We showed that Skin acidification is still under development during the first weeks of life. While the basal epidermal barrier is competent immediately after birth, the SC is less hydrated in the first 2 weeks of postnatal life. Similar continuous decreasing water content towards the surface for all age groups was observed, whereas this gradient was lower for the newborns. Dynamic changes in the amounts of the natural moisturizing factor constituents were revealed in the period of infancy. Conclusions We demonstrated the relation of formation of an acidic pH as well as underlying mechanisms in the induction of a fully hydrated SC over the first weeks of human life as a dynamic functional adaptation.
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comprar practical aspects of cosmetic testing how to set up a scientific study in Skin Physiology joachim w fluhr 9783642050664 springer
2010Co-Authors: Joachim W FluhAbstract:Tienda online donde Comprar Practical Aspects Of Cosmetic Testing. How To Set Up A Scientific Study In Skin Physiology al precio 151,19 € de Joachim W. Fluhr, tienda de Libros de Medicina, Libros de Cirugia - Cirugia plastica y reparadora
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silver loaded seaweed based cellulosic fiber improves epidermal Skin Physiology in atopic dermatitis safety assessment mode of action and controlled randomized single blinded exploratory in vivo study
Experimental Dermatology, 2009Co-Authors: Joachim W Fluh, Doreen Kowatzki, Maria Eternitz, Andrea Aue, Joerg Osse, Pete Elsne, Utachristina HipleAbstract:BACKGROUND: The epidermal part of the Skin is the major interface between the internal body and the external environment. The Skin has a specific Physiology and is to different degrees adapted for protection against multiple exogenous stress factors. Clothing is the material with the longest and most intensive contact to human Skin. It plays a critical role especially in inflammatory dermatoses or Skin conditions with an increased susceptibility of bacterial and fungal infections like atopic dermatitis. Previously, we have shown a dose-dependent antibacterial and antifungal activity of silver-loaded seaweed-based cellulosic fibres. AIM OF THE STUDY: We studied the mode of action of silver-loaded seaweed-based cellulosic fiber and performed a broad safety assessment. The principal aim was to analyse the effects of wearing the textile on epidermal Skin Physiology in 37 patients with atopic dermatitis in a controlled, randomized single-blinded in vivo study. Furthermore, the sensitization potential was tested in a patch test in 111 panellists. RESULTS: We could demonstrate in vitro a dose-dependent scavenging of induced reactive oxygen species by silver-loaded seaweed-based cellulosic fibers. Safety assessment of these fibres showed no detectable release of silver ions. Furthermore, ex vivo assessment after 24 h application both in healthy volunteers and patients with atopic dermatitis by sequential tape stripping and subsequently raster electron microscopy and energy dispersive microanalysis analysis revealed no detectable amounts of silver in any of stratum corneum layers. Serum analysis of silver showed no detectable levels. The in vivo patch testing of 111 volunteers revealed no sensitization against different SeaCell Active (SeaCell GmbH, Rudolstadt, Germany) containing fabrics. The in vivo study on 37 patients with known atopic dermatitis and mild-to-moderate eczema on their arms were randomly assigned to either silver-loaded seaweed fibre T-shirts or to cotton T-shirts for 8 weeks. A significant reduction in Staphylococcus aureus colonization was detectable for the silver T-shirts compared with cotton T-shirts without any changes in non-pathogenic surface bacteria colonization. Furthermore, a more pronounced improvement in barrier function (transepidermal water loss) was observed in mildly involved eczema areas during the first 4 weeks of the study. Stratum corneum hydration and surface pH improved in both treatment groups over time. CONCLUSION: The tested silver-loaded seaweed fibre can be regarded as safe and seams to be suited for application in bio-active textiles in atopic dermatitis based on its positive in vivo activity.
Ji Xu - One of the best experts on this subject based on the ideXlab platform.
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the role of connexins in ear and Skin Physiology functional insights from disease associated mutations
Biochimica et Biophysica Acta, 2013Co-Authors: Ji Xu, Bruce J. NicholsonAbstract:Abstract Defects in several different connexins have been associated with several different diseases. The most common of these is deafness, where a few mutations in connexin (Cx) 26 have been found to contribute to over 50% of the incidence of non-syndromic deafness in different human populations. Other mutations in Cx26 or Cx30 have also been associated with various Skin phenotypes linked to deafness (palmoplanta keratoderma, Bart–Pumphrey syndrome, Vohwinkel syndrome, keratitis–ichthyosis–deafness syndrome, etc.). The large array of disease mutants offers unique opportunities to gain insights into the underlying function of gap junction proteins and their channels in the normal and pathogenic physiologies of the cochlea and epidermis. This review focuses on those mutants where the impact on channel function has been assessed, and correlated with the disease phenotype, or organ function in knock-out mouse models. These approaches have provided evidence supporting a role of gap junctions and hemichannels in K+ removal and recycling in the ear, as well as possible roles for nutrient passage, in the cochlea. In contrast, increases in hemichannel opening leading to increased cell death, were associated with several keratitis–ichthyosis–deafness syndrome Skin disease/hearing mutants. In addition to providing clues for therapeutic strategies, these findings allow us to better understand the specific functions of connexin channels that are important for normal tissue function. This article is part of a Special Issue entitled: The communicating junctions, roles and dysfunctions.
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The role of connexins in ear and Skin Physiology — Functional insights from disease-associated mutations
Biochimica et Biophysica Acta, 2012Co-Authors: Ji Xu, Bruce J. NicholsonAbstract:Abstract Defects in several different connexins have been associated with several different diseases. The most common of these is deafness, where a few mutations in connexin (Cx) 26 have been found to contribute to over 50% of the incidence of non-syndromic deafness in different human populations. Other mutations in Cx26 or Cx30 have also been associated with various Skin phenotypes linked to deafness (palmoplanta keratoderma, Bart–Pumphrey syndrome, Vohwinkel syndrome, keratitis–ichthyosis–deafness syndrome, etc.). The large array of disease mutants offers unique opportunities to gain insights into the underlying function of gap junction proteins and their channels in the normal and pathogenic physiologies of the cochlea and epidermis. This review focuses on those mutants where the impact on channel function has been assessed, and correlated with the disease phenotype, or organ function in knock-out mouse models. These approaches have provided evidence supporting a role of gap junctions and hemichannels in K+ removal and recycling in the ear, as well as possible roles for nutrient passage, in the cochlea. In contrast, increases in hemichannel opening leading to increased cell death, were associated with several keratitis–ichthyosis–deafness syndrome Skin disease/hearing mutants. In addition to providing clues for therapeutic strategies, these findings allow us to better understand the specific functions of connexin channels that are important for normal tissue function. This article is part of a Special Issue entitled: The communicating junctions, roles and dysfunctions.
