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Werner Fuchs - One of the best experts on this subject based on the ideXlab platform.

  • Bioavailability of essential trace elements and their impact on anaerobic digestion of Slaughterhouse Waste
    Biochemical Engineering Journal, 2015
    Co-Authors: Markus Ortner, Günther Bochmann, Michael Rameder, Lydia Rachbauer, Werner Fuchs
    Abstract:

    Abstract Slaughterhouse Waste is an energy rich feedstock suitable for anaerobic digestion processes. However, chemical characterization showed a deficiency in essential trace elements which are critical for optimal performance of the process. Hence this study investigated the degree of bioavailability of trace elements in four semi-continuous lab-scale AD tests accepting Slaughterhouse Waste under mesophilic conditions (38 °C) and a moderate organic loading rate of 2.2 kg/m3 d. Parameters, such as volatile fatty acid (VFA) concentration, COD removal rate and specific methane yield were compared to the results of sequential extraction analysis. The highest methane yield (250–275 Nm3/t COD), lowest accumulation of VFA (

  • Efficient anaerobic mono-digestion of N-rich Slaughterhouse Waste: influence of ammonia, temperature and trace elements.
    Bioresource technology, 2014
    Co-Authors: Markus Ortner, Kerstin Leitzinger, Sören Skupien, Günther Bochmann, Werner Fuchs
    Abstract:

    Three mono-digestion experiments treating Slaughterhouse Waste with high TKN concentration (∼11g/kg) were applied in lab-scale at mesophilic and psychrophilic conditions to study the impact of high ammonia concentrations and additives. Precipitation of sulphur by addition of ferrous chloride did not influence process behaviour, whereas supplementation of trace elements significantly improved process stability by reducing volatile fatty acid concentration towards zero. The limit of NH4-N concentration causing a rise of VFAs to 19,000mg/l and reduction of methane by 25% was found between 7.7 and 9.1g/kg which correspond to NH3 concentrations of 830-1060mg/l. Psychrophilic operation (25°C) lowered inhibitory NH3 concentration to 140mg/l, but process performance was stable only at low OLR of 0.4kgVS/m(3)d. Robust performance at highest possible NH4-N concentration (7.7g/kg), low VFA accumulation and satisfying methane yield of about 280Nm(3)/t COD was observed at OLR of 2.5kgVS/m(3)d at 37°C.

Chang Chen - One of the best experts on this subject based on the ideXlab platform.

  • anaerobic digestion of lipid rich swine Slaughterhouse Waste methane production performance long chain fatty acids profile and predominant microorganisms
    Bioresource Technology, 2018
    Co-Authors: Zhifang Ning, Guangqing Liu, Han Zhang, Ruihong Zhang, Chang Chen
    Abstract:

    Abstract This study investigated methane production, long-chain fatty acids (LCFAs) profile, and predominant microorganisms in anaerobic digestion (AD) of lipid-rich swine Slaughterhouse Waste (SSW). The maximum methane yield was 999.2 mL/g VS. LCFAs, as inhibitory hydrolysis products, accumulated first to 1165 mg/L on day 3, and then decreased sharply to 125.7 mg/L on day 9, and finally were degraded to 20 mg/L on day 27. Linoleic acid (C18:2), oleic acid (C18:1) and palmitic acid (C16:0) were the dominant LCFAs. The easy conversion of C18:1 to C16:0 compared with difficult degradation of C16:0 resulted in an increase of C16:0 on day 4–6. Predominant microorganisms were Clostridium, Syntrophomonas and Methanospirillum. This study proved the high methane potential of lipid-rich SSW and gained insights into the degradation process by analysis of intermediates of LCFAs and predominant microorganisms. The results can provide valuable guidance for efficient utilization of this Waste to produce methane in future.

  • Effect of Lipase Hydrolysis on Biomethane Production from Swine Slaughterhouse Waste in China
    Energy & Fuels, 2016
    Co-Authors: Zhifang Ning, Yan Huang, Guangqing Liu, Chang Chen
    Abstract:

    The purpose of this study was to evaluate biomethane production in anaerobic digestion of swine Slaughterhouse Waste (SSW) and the effect of lipase pretreatment on biomethane yield. Lipase extracted from porcine pancreas was employed to hydrolyze SSW at 37 °C for 24 h. Three different lipase concentrations of 0.04, 0.2, and 0.4% (w/v) were adopted. Anaerobic digestion was carried out in batch style for 50 days. The results showed that the highest experimental biomethane yield (EMY) of 851.6 mL/g of VSadded was achieved when the lipase concentration of 0.2% (w/v) was used, which was significantly higher (18.4%; α < 0.05) than that of the untreated group. Furthermore, the highest biodegradability (Bd) of 72.7% was also achieved at 0.2% (w/v) lipase-pretreated group. Results proved that the conversion of SSW to biomethane was improved through pretreatment of lipase hydrolysis and offered a promising method for disposal of SSW.

Jürgen H. Thiele - One of the best experts on this subject based on the ideXlab platform.

  • isolation and characterization of two glycerol fermenting clostridial strains from a pilot scale anaerobic digester treating high lipid content Slaughterhouse Waste
    Journal of Applied Microbiology, 1999
    Co-Authors: Graeme N. Jarvis, C. Strömpl, E. R. B. Moore, Jürgen H. Thiele
    Abstract:

    G.N. J ARVIS, C. STROMPL, E.R.B. MOORE AND J.H. THIELE. 1999. Two obligately anaerobic bacterial strains were isolated from the contents of a pilot scale, anaerobic digester treating Slaughterhouse Waste with a high protein and lipid content. The isolates, LIP1 and MW8, were characterized as spore-forming, Gram-positive rods, capable of fermenting glycerol. Isolate LIP1 was also observed to be lipolytic and was able to hydrolyse tallow and olive oil. Both isolates grew optimally at 37 °C and formed either acetate and formate (LIP1), or acetate and butyrate (MW8), as major glycerol fermentation products. Both isolates produced ethanol as the major reduced fermentation end-product. Neither MW8 nor LIP1 had growth and metabolism inhibited by the addition of stearic acid at concentrations normally considered bactericidal. Analysis of the 16S rRNA gene sequences, in conjunction with the phenotypic data, confirmed that the isolates are members of the genus Clostridium (sensu lato), clustering with species of clostridial clusters I (MW8) and XIVa (LIP1).

  • Isolation and characterization of two glycerol‐fermenting clostridial strains from a pilot scale anaerobic digester treating high lipid‐content Slaughterhouse Waste
    Journal of applied microbiology, 1999
    Co-Authors: Graeme N. Jarvis, C. Strömpl, E. R. B. Moore, Jürgen H. Thiele
    Abstract:

    Two obligately anaerobic bacterial strains were isolated from the contents of a pilot scale, anaerobic digester treating Slaughterhouse Waste with a high protein and lipid content. The isolates, LIP1 and MW8, were characterized as spore-forming, Gram-positive rods, capable of fermenting glycerol. Isolate LIP1 was also observed to be lipolytic and was able to hydrolyse tallow and olive oil. Both isolates grew optimally at 37 degrees C and formed either acetate and formate (LIP1), or acetate and butyrate (MW8), as major glycerol fermentation products. Both isolates produced ethanol as the major reduced fermentation end-product. Neither MW8 nor LIP1 had growth and metabolism inhibited by the addition of stearic acid at concentrations normally considered bactericidal. Analysis of the 16S rRNA gene sequences, in conjunction with the phenotypic data, confirmed that the isolates are members of the genus Clostridium (sensu lato), clustering with species of clostridial clusters I (MW8) and XIVa (LIP1).

  • Isolation and characterization of two glycerol‐fermenting clostridial strains from a pilot scale anaerobic digester treating high lipid‐content Slaughterhouse Waste
    Journal of applied microbiology, 1999
    Co-Authors: Graeme N. Jarvis, C. Strömpl, E. R. B. Moore, Jürgen H. Thiele
    Abstract:

    G.N. J ARVIS, C. STROMPL, E.R.B. MOORE AND J.H. THIELE. 1999. Two obligately anaerobic bacterial strains were isolated from the contents of a pilot scale, anaerobic digester treating Slaughterhouse Waste with a high protein and lipid content. The isolates, LIP1 and MW8, were characterized as spore-forming, Gram-positive rods, capable of fermenting glycerol. Isolate LIP1 was also observed to be lipolytic and was able to hydrolyse tallow and olive oil. Both isolates grew optimally at 37 °C and formed either acetate and formate (LIP1), or acetate and butyrate (MW8), as major glycerol fermentation products. Both isolates produced ethanol as the major reduced fermentation end-product. Neither MW8 nor LIP1 had growth and metabolism inhibited by the addition of stearic acid at concentrations normally considered bactericidal. Analysis of the 16S rRNA gene sequences, in conjunction with the phenotypic data, confirmed that the isolates are members of the genus Clostridium (sensu lato), clustering with species of clostridial clusters I (MW8) and XIVa (LIP1).

Gunnar Lidén - One of the best experts on this subject based on the ideXlab platform.

  • Semi-continuous co-digestion of solid Slaughterhouse Waste, manure, and fruit and vegetable Waste
    Renewable Energy, 2008
    Co-Authors: René Alvarez, Gunnar Lidén
    Abstract:

    The potential of semi-continuous mesophilic anaerobic digestion (AD) for the treatment of solid Slaughterhouse Waste, fruit-vegetable Wastes, and manure in a co-digestion process has been experimentally evaluated. A study was made at laboratory scale using four 2 L reactors working semi-continuously at 35 degrees C. The effect of the organic loading rate (OLR) was initially examined (using equal proportion of the three components on a volatile solids, VS, basis). Anaerobic co-digestion with OLRs in the range 0.3-1.3 kg VS m(-3) d(-1) resulted in methane yields of 0.3 m(3) kg(-1) VS added, with a methane content in the biogas of 54-56%. However, at a further increased loading, the biogas production decreased and there was a reduction in the methane yield indicating organic overload or insufficient buffering capacity in the digester. In the second part of the investigation, co-digestion was studied in a mixture experiment using 10 different feed compositions. The digestion of mixed substrates was in all cases better than that of the pure substrates, with the exception of the mixture of equal amounts of (VS/VS) solid cattle-swine Slaughterhouse Waste (SCSSW) with fruit and vegetable Waste (FVW). For all other mixtures, the steady-state biogas production for the mixture was in the range 1.1-1.6 L d(-1), with a methane content of 50-57% after 60 days of operation. The methane yields were in the range 0.27-0.35 m(3) kg(-1) VS added and VS reductions of more than 50% and up to 67% were obtained. (Less)

Markus Ortner - One of the best experts on this subject based on the ideXlab platform.

  • Bioavailability of essential trace elements and their impact on anaerobic digestion of Slaughterhouse Waste
    Biochemical Engineering Journal, 2015
    Co-Authors: Markus Ortner, Günther Bochmann, Michael Rameder, Lydia Rachbauer, Werner Fuchs
    Abstract:

    Abstract Slaughterhouse Waste is an energy rich feedstock suitable for anaerobic digestion processes. However, chemical characterization showed a deficiency in essential trace elements which are critical for optimal performance of the process. Hence this study investigated the degree of bioavailability of trace elements in four semi-continuous lab-scale AD tests accepting Slaughterhouse Waste under mesophilic conditions (38 °C) and a moderate organic loading rate of 2.2 kg/m3 d. Parameters, such as volatile fatty acid (VFA) concentration, COD removal rate and specific methane yield were compared to the results of sequential extraction analysis. The highest methane yield (250–275 Nm3/t COD), lowest accumulation of VFA (

  • Efficient anaerobic mono-digestion of N-rich Slaughterhouse Waste: influence of ammonia, temperature and trace elements.
    Bioresource technology, 2014
    Co-Authors: Markus Ortner, Kerstin Leitzinger, Sören Skupien, Günther Bochmann, Werner Fuchs
    Abstract:

    Three mono-digestion experiments treating Slaughterhouse Waste with high TKN concentration (∼11g/kg) were applied in lab-scale at mesophilic and psychrophilic conditions to study the impact of high ammonia concentrations and additives. Precipitation of sulphur by addition of ferrous chloride did not influence process behaviour, whereas supplementation of trace elements significantly improved process stability by reducing volatile fatty acid concentration towards zero. The limit of NH4-N concentration causing a rise of VFAs to 19,000mg/l and reduction of methane by 25% was found between 7.7 and 9.1g/kg which correspond to NH3 concentrations of 830-1060mg/l. Psychrophilic operation (25°C) lowered inhibitory NH3 concentration to 140mg/l, but process performance was stable only at low OLR of 0.4kgVS/m(3)d. Robust performance at highest possible NH4-N concentration (7.7g/kg), low VFA accumulation and satisfying methane yield of about 280Nm(3)/t COD was observed at OLR of 2.5kgVS/m(3)d at 37°C.