The Experts below are selected from a list of 297 Experts worldwide ranked by ideXlab platform

Francesca Cappitelli - One of the best experts on this subject based on the ideXlab platform.

  • altered expression level of escherichia coli proteins in response to treatment with the antifouling agent zosteric acid Sodium Salt
    Environmental Microbiology, 2012
    Co-Authors: Federica Villa, William Remelli, Fabio Forlani, Alberto Vitali, Francesca Cappitelli
    Abstract:

    Summary Zosteric acid Sodium Salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid Sodium Salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P < 0.001) altered expression level in response to the antifoulant. The proteomic signatures of zosteric acid Sodium Salt-treated cells are characterized by stress-associated (e.g. AhpC, OsmC, SodB, GroES, IscU, DnaK), motility-related (FliC), quorum-sensing-associated (LuxS) and metabolism/biosynthesis-related (e.g. PptA, AroA, FabD, FabB, GapA) proteins. Consistent with the overexpression of LuxS enzyme, the antifouling agent increased autoinducer-2 (AI-2) concentration by twofold. Moreover, treated cells experienced a statistically significant but modest increase of reactive oxygen species (+ 23%), tryptophanase (1.2-fold) and indole (1.2-fold) synthesis. Overall, our data suggest that zosteric acid Sodium Salt acts as environmental cue leading to global stress on E. coli cells, which favours the expression of various protective proteins, the AI-2 production and the synthesis of flagella, to escape from adverse conditions.

  • Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid Sodium Salt
    Environmental Microbiology, 2011
    Co-Authors: Federica Villa, William Remelli, Fabio Forlani, Alberto Vitali, Francesca Cappitelli
    Abstract:

    Summary Zosteric acid Sodium Salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid Sodium Salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P 

  • Efficacy of zosteric acid Sodium Salt on the yeast biofilm model Candida albicans.
    Microbial Ecology, 2011
    Co-Authors: Federica Villa, Betsey Pitts, Philip S. Stewart, Barbara Giussani, Simone Roncoroni, Domenico Albanese, Carmen Giordano, Marta Tunesi, Francesca Cappitelli
    Abstract:

    Candida albicans is the most notorious and the most widely studied yeast biofilm former. Design of experiments (DoE) showed that 10 mg/L zosteric acid Sodium Salt reduced C. albicans adhesion and the subsequent biofilm formation by at least 70%, on both hydrophilic and hydrophobic surfaces of 96-well plates. Indeed, biofilm imaging revealed the dramatic impact of zosteric acid Sodium Salt on biofilm thickness and morphology, due to the inability of the cells to form filamentous structures while remaining metabolically active. In the same way, 10 mg/L zosteric acid Sodium Salt inhibited C. albicans biofilm formation when added after the adhesion phase. Contrary to zosteric acid Sodium Salt, methyl zosterate did not affect yeast biofilm. In addition, zosteric acid Sodium Salt enhanced sensitivity to chlorhexidine, chlorine, hydrogen peroxide, and cis-2-decenoic acid, with a reduction of 0.5 to 8 log units. Preliminary in vitro studies using suitable primary cell based models revealed that zosteric acid Sodium Salt did not compromise the cellular activity, adhesion, proliferation or morphology of either the murine fibroblast line L929 or the human osteosarcoma line MG-63. Thus the use of zosteric acid Sodium Salt could provide a suitable, innovative, preventive, and integrative approach to preventing yeast biofilm formation.

Federica Villa - One of the best experts on this subject based on the ideXlab platform.

  • altered expression level of escherichia coli proteins in response to treatment with the antifouling agent zosteric acid Sodium Salt
    Environmental Microbiology, 2012
    Co-Authors: Federica Villa, William Remelli, Fabio Forlani, Alberto Vitali, Francesca Cappitelli
    Abstract:

    Summary Zosteric acid Sodium Salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid Sodium Salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P < 0.001) altered expression level in response to the antifoulant. The proteomic signatures of zosteric acid Sodium Salt-treated cells are characterized by stress-associated (e.g. AhpC, OsmC, SodB, GroES, IscU, DnaK), motility-related (FliC), quorum-sensing-associated (LuxS) and metabolism/biosynthesis-related (e.g. PptA, AroA, FabD, FabB, GapA) proteins. Consistent with the overexpression of LuxS enzyme, the antifouling agent increased autoinducer-2 (AI-2) concentration by twofold. Moreover, treated cells experienced a statistically significant but modest increase of reactive oxygen species (+ 23%), tryptophanase (1.2-fold) and indole (1.2-fold) synthesis. Overall, our data suggest that zosteric acid Sodium Salt acts as environmental cue leading to global stress on E. coli cells, which favours the expression of various protective proteins, the AI-2 production and the synthesis of flagella, to escape from adverse conditions.

  • Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid Sodium Salt
    Environmental Microbiology, 2011
    Co-Authors: Federica Villa, William Remelli, Fabio Forlani, Alberto Vitali, Francesca Cappitelli
    Abstract:

    Summary Zosteric acid Sodium Salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid Sodium Salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P 

  • Efficacy of zosteric acid Sodium Salt on the yeast biofilm model Candida albicans.
    Microbial Ecology, 2011
    Co-Authors: Federica Villa, Betsey Pitts, Philip S. Stewart, Barbara Giussani, Simone Roncoroni, Domenico Albanese, Carmen Giordano, Marta Tunesi, Francesca Cappitelli
    Abstract:

    Candida albicans is the most notorious and the most widely studied yeast biofilm former. Design of experiments (DoE) showed that 10 mg/L zosteric acid Sodium Salt reduced C. albicans adhesion and the subsequent biofilm formation by at least 70%, on both hydrophilic and hydrophobic surfaces of 96-well plates. Indeed, biofilm imaging revealed the dramatic impact of zosteric acid Sodium Salt on biofilm thickness and morphology, due to the inability of the cells to form filamentous structures while remaining metabolically active. In the same way, 10 mg/L zosteric acid Sodium Salt inhibited C. albicans biofilm formation when added after the adhesion phase. Contrary to zosteric acid Sodium Salt, methyl zosterate did not affect yeast biofilm. In addition, zosteric acid Sodium Salt enhanced sensitivity to chlorhexidine, chlorine, hydrogen peroxide, and cis-2-decenoic acid, with a reduction of 0.5 to 8 log units. Preliminary in vitro studies using suitable primary cell based models revealed that zosteric acid Sodium Salt did not compromise the cellular activity, adhesion, proliferation or morphology of either the murine fibroblast line L929 or the human osteosarcoma line MG-63. Thus the use of zosteric acid Sodium Salt could provide a suitable, innovative, preventive, and integrative approach to preventing yeast biofilm formation.

Adam Szeląg - One of the best experts on this subject based on the ideXlab platform.

William Remelli - One of the best experts on this subject based on the ideXlab platform.

  • altered expression level of escherichia coli proteins in response to treatment with the antifouling agent zosteric acid Sodium Salt
    Environmental Microbiology, 2012
    Co-Authors: Federica Villa, William Remelli, Fabio Forlani, Alberto Vitali, Francesca Cappitelli
    Abstract:

    Summary Zosteric acid Sodium Salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid Sodium Salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P < 0.001) altered expression level in response to the antifoulant. The proteomic signatures of zosteric acid Sodium Salt-treated cells are characterized by stress-associated (e.g. AhpC, OsmC, SodB, GroES, IscU, DnaK), motility-related (FliC), quorum-sensing-associated (LuxS) and metabolism/biosynthesis-related (e.g. PptA, AroA, FabD, FabB, GapA) proteins. Consistent with the overexpression of LuxS enzyme, the antifouling agent increased autoinducer-2 (AI-2) concentration by twofold. Moreover, treated cells experienced a statistically significant but modest increase of reactive oxygen species (+ 23%), tryptophanase (1.2-fold) and indole (1.2-fold) synthesis. Overall, our data suggest that zosteric acid Sodium Salt acts as environmental cue leading to global stress on E. coli cells, which favours the expression of various protective proteins, the AI-2 production and the synthesis of flagella, to escape from adverse conditions.

  • Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid Sodium Salt
    Environmental Microbiology, 2011
    Co-Authors: Federica Villa, William Remelli, Fabio Forlani, Alberto Vitali, Francesca Cappitelli
    Abstract:

    Summary Zosteric acid Sodium Salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid Sodium Salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P 

Fabio Forlani - One of the best experts on this subject based on the ideXlab platform.

  • altered expression level of escherichia coli proteins in response to treatment with the antifouling agent zosteric acid Sodium Salt
    Environmental Microbiology, 2012
    Co-Authors: Federica Villa, William Remelli, Fabio Forlani, Alberto Vitali, Francesca Cappitelli
    Abstract:

    Summary Zosteric acid Sodium Salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid Sodium Salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P < 0.001) altered expression level in response to the antifoulant. The proteomic signatures of zosteric acid Sodium Salt-treated cells are characterized by stress-associated (e.g. AhpC, OsmC, SodB, GroES, IscU, DnaK), motility-related (FliC), quorum-sensing-associated (LuxS) and metabolism/biosynthesis-related (e.g. PptA, AroA, FabD, FabB, GapA) proteins. Consistent with the overexpression of LuxS enzyme, the antifouling agent increased autoinducer-2 (AI-2) concentration by twofold. Moreover, treated cells experienced a statistically significant but modest increase of reactive oxygen species (+ 23%), tryptophanase (1.2-fold) and indole (1.2-fold) synthesis. Overall, our data suggest that zosteric acid Sodium Salt acts as environmental cue leading to global stress on E. coli cells, which favours the expression of various protective proteins, the AI-2 production and the synthesis of flagella, to escape from adverse conditions.

  • Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid Sodium Salt
    Environmental Microbiology, 2011
    Co-Authors: Federica Villa, William Remelli, Fabio Forlani, Alberto Vitali, Francesca Cappitelli
    Abstract:

    Summary Zosteric acid Sodium Salt is a powerful antifouling agent. However, the mode of its antifouling action has not yet been fully elucidated. Whole cell proteome of Escherichia coli was analysed to study the different protein patterns expressed by the surface-exposed planktonic cells without and with sublethal concentrations of the zosteric acid Sodium Salt. Proteomic analysis revealed that at least 27 proteins showed a significant (19 upregulated and 8 downregulated, P