The Experts below are selected from a list of 9720 Experts worldwide ranked by ideXlab platform
Maria José Vieira Fonseca - One of the best experts on this subject based on the ideXlab platform.
-
The role of Soybean Extracts and isoflavones in hormone-dependent breast cancer: aromatase activity and biological effects.
Food & function, 2017Co-Authors: Cristina Amaral, Maria José Vieira Fonseca, Maria Regina Torqueti Toloi, Luís Daniel Ferreira Vasconcelos, Georgina Correia-da-silva, Natércia TeixeiraAbstract:Estrogen receptor-positive (ER+) breast cancer is the most common cause of cancer death in women worldwide. Nowadays, the relationship between soya diet and breast cancer is controversial due to the unknown role of its isoflavones, genistein (G) and daidzein (D). In this work, we investigated not only the anti-tumor properties of a Soybean Extract (NBSE) but also whether the biotransformation of Extract (BSE) by the fungus Aspergillus awamori increased its effectiveness. The BSE showed a stronger anti-aromatase activity and anti-proliferative efficacy in ER+ aromatase-overexpressing breast cancer cells. D and G were weak aromatase inhibitors, but inhibited cancer cell growth, being G the isoflavone that contributed to the BSE-induced effects. This work demonstrated that the biotransformation increased the anti-aromatase activity and the anti-tumoral efficacy of Soybean Extract in breast cancer cells. Moreover, it elucidated the potential use of soya in the prevention and/or treatment of ER+ breast cancer.
-
Biotransformed Soybean Extract induces cell death of estrogen-dependent breast cancer cells by modulation of apoptotic proteins
Nutrition and cancer, 2015Co-Authors: Bianca Stocco, Maria José Vieira Fonseca, Karina Alves Toledo, Helen Figueiredo Fumagalli, Francine J. Bianchini, Vanessa Silveira Fortes, Maria Regina Torqueti ToloiAbstract:The process of Soybean biotransformation increases the quantity of isoflavones (daidzein and genistein), which besides being considered an alternative to estroprogestive hormone replacement therapy (HRT), are able of hindering the growth and development of tumor cells. We investigated the effects of Soybean Extract biotransformed by fungus on estrogen-dependent (MCF-7) and nondependent (SK-BR-3) breast cell lines. Cells were treated with different concentrations of biotransformed (BSE) and nonbiotransformed Soybean Extract (SE), or daidzein (D) and genistein (G) patterns isolated and in combination (D + G). Afterwards, we analyzed cell viability by MTT assay, phosphatidylserine exposure and cell permeability by flow cytometry; expression of apoptotic proteins by Western blotting. BSE promoted reduction in cell viability and increase in DNA degradation in both cell lines. In addition, we verified increase in cell permeability and in the expression of phosphatidylserine, as well as modulation in the express...
-
Biotransformed Soybean Extract (BSE) inhibits melanoma cell growth and viability in vitro: involvement of nuclear factor-kappa B signaling.
PloS one, 2014Co-Authors: Fernanda Maria Pinto Vilela, Maria José Vieira Fonseca, Vanessa Silveira Fortes, Deeba N. Syed, Jean Christopher Chamcheu, Laura A. Calvo-castro, Hasan MukhtarAbstract:Melanoma is recognized as one of the most aggressive cancers with a relatively high propensity for metastasis. The prognosis of melanoma remains poor in spite of treatment advances, emphasizing the importance of additional preventive measures. Isoflavonoids have become not only potential chemopreventive, but also important therapeutic natural agents. We evaluated the antiproliferative and proapoptotic properties of biotransformed Soybean Extract (BSE) in A375 melanoma cells. Previous analyses demonstrated that the concentration of daidzein, genistein and aminoacids/peptides present in BSE, fermented by Aspergillus awamori is much higher than in the non biotransformed Extract (NBSE). Experiments comparing the efficacy of the Extracts in preventing cancer cell growth showed that treatment (24 h) of aggressive melanoma cells (A375 and 451Lu) with BSE resulted in a dose-dependent inhibition of growth and viability. In contrast, treatment with similar doses of NBSE failed to inhibit melanoma cell viability. Further studies in A375 cells showed that decrease in cell viability with BSE treatment (1.5–1.9 mg/ml; 24 h) was associated with induction of apoptosis. Immunoblot analysis revealed that BSE treatment resulted in induction of PARP cleavage, activation of caspase-3, -7, and -8 and increased expression of TRAIL and its receptor DR4. BSE did not activate the intrinsic apoptotic pathway in A375 cells, as no change was observed in caspase-9 expression. The expression of Bcl-2 apoptotic proteins such as Bid and Bax remained unaffected with BSE treated cells. Interestingly, we also showed that BSE treatment increased the phosphorylation and activation of IKK, IκBα degradation and p65/NF-κB translocation to the nucleus, and that stimulation of the NF-???B pathway was required for BSE-induced apoptosis of A375 cells. Our findings indicate that the biotransformation of Soybean plays a crucial role in the Extract anti-cancer effect observed in melanoma cells. However, further studies are warranted to define the active anti-cancer agent(s) present in BSE.
-
Abstract 607: Biotransformed Soybean Extract (BSE) inhibits melanoma cell growth and viability through activation of the extrinsic apoptotic pathway.
Molecular and Cellular Biology, 2013Co-Authors: Fernanda Maria Pinto Vilela, Maria José Vieira Fonseca, Vanessa Silveira Fortes, Deeba N. Syed, Jean Christopher Chamcheu, Laura C. Castro, Hasan MukhtarAbstract:Melanoma is recognized as one of the most aggressive cancers with relatively high propensity for metastasis. The prognosis of melanoma remains poor in spite of treatment advances, emphasizing the importance of additional preventive measures. Isoflavonoids have become not only potential chemopreventive, but also important therapeutic natural agents. In this study, employing A375 melanoma cells, we evaluated the antiproliferative and proapoptotic properties of biotransformed Soybean Extract (BSE). Chromatographic analysis and ninhydrin-based assays demonstrated that the concentration of daidzein, genistein and aminoacids/peptides present in BSE, fermented by Aspergillus awamori is much higher than in the non biotransformed Extract (NBSE). Initial experiments comparing the efficacy of the Extracts in preventing cancer cell growth showed that treatment of highly invasive melanoma cells (A375 and 451Lu) with BSE resulted in a dose-dependent inhibition of growth and viability, 24 h post-treatment, as assessed by 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazoliumbromide assay. In contrast, treatment with similar doses of NBSE failed to inhibit melanoma cell viability. Further studies in A375 melanoma cells showed that decrease in cell viability with BSE (1.5-1.9 mg/ml; 24 h) treatment was associated with induction of apoptosis. Immunoblot analysis revealed that BSE treatment resulted in (i) induction of PARP cleavage and activation of caspase-3, -7, and -8; (ii) decreased FLIP L ; and (iii) increased expression of TRAIL and its receptor DR4. BSE did not activate the intrinsic apoptotic pathway in A375 melanoma cells, as no change was observed in caspase -9 expression. In addition, the expression of Bcl-2 family of apoptotic proteins such as Bax, Bcl-2 remained unaffected with BSE treated cells. BSE treatment increased the phosphorylation and activation of IKK, IκBα degradation and p65/NFκB translocation to the nucleus suggesting that NFκB signaling may be involved in BSE mediated apoptosis. Taken together, our findings indicate that the biotransformation of Soybean plays a crucial role in the anti-cancer effect of the Extract observed in melanoma cells and further studies are warranted to define the active agent present in BSE and define its mechanism. Citation Format: Fernanda Maria Pinto Vilela, Deeba N. Syed, Jean-Christopher Chamcheu, Laura C. Castro, Vanessa S. Fortes, Maria Jose V. Fonseca, Hasan Mukhtar. Biotransformed Soybean Extract (BSE) inhibits melanoma cell growth and viability through activation of the extrinsic apoptotic pathway. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 607. doi:10.1158/1538-7445.AM2013-607 Note: This abstract was not presented at the AACR Annual Meeting 2013 because the presenter was unable to attend.
-
Spray drying of the Soybean Extract: Effects on chemical properties and antioxidant activity
LWT - Food Science and Technology, 2008Co-Authors: Sandra R Georgetti, Rúbia Casagrande, Claudia R. F. Souza, Wanderley Pereira De Oliveira, Maria José Vieira FonsecaAbstract:Abstract Recent findings that many human chronic diseases are associated with oxidative stresses have instigated the search for dietary antioxidants. Isoflavones, a special phenolic group found in Soybean, have been found to act as antioxidant in some model systems. The aim of this work was the evaluation the effects of spray drying on the chemical and biological properties of Soybean Extract. The total polyphenol contents in the concentrated Soybean Extract (CSE) and spray dried Soybean Extract (SDSE) were determined by Folin–Ciocalteau method and HPLC was utilized for the quantification of the genistein, active compound used as a chemical marker. The evaluation of antioxidant activity of CSE and SDSE was assessed by the hydrogen-donor ability to 2,2-diphenyl-1-picrylhydrazyl radical (DPPH ). The results indicated that the inlet gas temperature showed significant effect on the total polyphenol, protein and genistein contents of the dried Extracts. The SDSE obtained this work showed high antioxidant activity, opening perspectives for its use as food additive and/or ingredient.
Hyung Joo Suh - One of the best experts on this subject based on the ideXlab platform.
-
Protective effects of germinated and fermented Soybean Extract against tert-butyl hydroperoxide-induced hepatotoxicity in HepG2 cells and in rats.
Food & function, 2015Co-Authors: Eun Young Kim, Hyung Joo Suh, Ki Bae Hong, Hyeon Son ChoiAbstract:The aim of the current study is to investigate the antioxidant and hepatoprotective effects of germinated and fermented Soybean Extract (GFSE) on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in HepG2 cells and in the rat liver. High performance liquid chromatography (HPLC) analysis showed that genistin (3.40 ± 0.14 μg mg(-1)) was the most abundant isoflavone in the GFSE. Coumestrol (1.00 ± 0.04 μg mg(-1)), daidzin (0.78 ± 0.14 μg mg(-1)), genistein (0.68 ± 0.05 μg mg(-1)), glycitin (0.54 ± 0.02 μg mg(-1)), glycitein (0.41 ± 0.02 μg mg(-1)), and daidzein (0.02 ± 0.0 g mg(-1)) are also contained in decreasing order of content. GFSE significantly inhibited t-BHP-induced reactive oxygen species (ROS) production in HepG2 cells. This GFSE-induced ROS reduction was associated with the down-regulation of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), a pro-oxidant enzyme, and the up-regulation of the mRNA levels of antioxidant enzymes, including catalase, superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (Gpx) in the rat liver. In addition, increased levels of antioxidant enzyme mRNAs correlated with the enhanced enzymatic activities of SOD, catalase, and glutathione-S-transferase (GST). The antioxidant effect of GFSE was supported by the reduction in the levels of malondialdehyde (MDA), a hydroperoxide, and the serum levels of lactate dehydrogenase (LDH), a biomarker of cell damage, were also lowered by GFSE. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST), which are clinical biomarkers of liver function, were shown to be improved with GFSE administration. The effects of GFSE were attributable to an improvement in liver tissue morphology. Taken together, GFSE protected the liver from t-BHP-induced oxidative stress by regulating ROS-related enzymes. Our results suggest that GFSE might be a hepatoprotective source against oxidative stress.
-
Protective Effect of Germinated and Fermented Soybean Extract Against tert-butyl Hydroperoxide-Induced Hepatotoxicity in the Rats
The FASEB Journal, 2015Co-Authors: Eun Young Kim, Yooheon Park, Hyung Joo SuhAbstract:Current study was aimed to investigate antioxidant and hepatoprotective effects of germinated andfermented Soybean Extract (GFSE) on tert-butyl hydroperoxide(t-BHP)–induced oxidative stress in HepG...
Eun Young Kim - One of the best experts on this subject based on the ideXlab platform.
-
Protective effects of germinated and fermented Soybean Extract against tert-butyl hydroperoxide-induced hepatotoxicity in HepG2 cells and in rats.
Food & function, 2015Co-Authors: Eun Young Kim, Hyung Joo Suh, Ki Bae Hong, Hyeon Son ChoiAbstract:The aim of the current study is to investigate the antioxidant and hepatoprotective effects of germinated and fermented Soybean Extract (GFSE) on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in HepG2 cells and in the rat liver. High performance liquid chromatography (HPLC) analysis showed that genistin (3.40 ± 0.14 μg mg(-1)) was the most abundant isoflavone in the GFSE. Coumestrol (1.00 ± 0.04 μg mg(-1)), daidzin (0.78 ± 0.14 μg mg(-1)), genistein (0.68 ± 0.05 μg mg(-1)), glycitin (0.54 ± 0.02 μg mg(-1)), glycitein (0.41 ± 0.02 μg mg(-1)), and daidzein (0.02 ± 0.0 g mg(-1)) are also contained in decreasing order of content. GFSE significantly inhibited t-BHP-induced reactive oxygen species (ROS) production in HepG2 cells. This GFSE-induced ROS reduction was associated with the down-regulation of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), a pro-oxidant enzyme, and the up-regulation of the mRNA levels of antioxidant enzymes, including catalase, superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (Gpx) in the rat liver. In addition, increased levels of antioxidant enzyme mRNAs correlated with the enhanced enzymatic activities of SOD, catalase, and glutathione-S-transferase (GST). The antioxidant effect of GFSE was supported by the reduction in the levels of malondialdehyde (MDA), a hydroperoxide, and the serum levels of lactate dehydrogenase (LDH), a biomarker of cell damage, were also lowered by GFSE. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST), which are clinical biomarkers of liver function, were shown to be improved with GFSE administration. The effects of GFSE were attributable to an improvement in liver tissue morphology. Taken together, GFSE protected the liver from t-BHP-induced oxidative stress by regulating ROS-related enzymes. Our results suggest that GFSE might be a hepatoprotective source against oxidative stress.
-
Protective Effect of Germinated and Fermented Soybean Extract Against tert-butyl Hydroperoxide-Induced Hepatotoxicity in the Rats
The FASEB Journal, 2015Co-Authors: Eun Young Kim, Yooheon Park, Hyung Joo SuhAbstract:Current study was aimed to investigate antioxidant and hepatoprotective effects of germinated andfermented Soybean Extract (GFSE) on tert-butyl hydroperoxide(t-BHP)–induced oxidative stress in HepG...
Fuquan Yang - One of the best experts on this subject based on the ideXlab platform.
-
separation and purification of isoflavones from a crude Soybean Extract by high speed counter current chromatography
Journal of Chromatography A, 2001Co-Authors: Fuquan YangAbstract:A set of isoflavones with a broad range of polarity including daidzin, glycitin, genistin, acetyldaidzin, glycitein, acetylgenistin and daidzein was separated from a crude Soybean Extract by high-speed counter-current chromatography using a two-step operation. Three solvent systems were used: chloroform–methanol–water (4:3:2, v/v); chloroform–methanol–n-butanol–water (4:3:0.5:2, v/v); and methyl tert.-butyl ether–tetrahydrofuran–0.5% aqueous trifluoroacetic acid (2:2:0.15:4, v/v). The first solvent system was used for separating less polar isoflavones and the second for more polar isoflavones by eluting the lower organic phase. Genistin and glycitin, which were only partially resolved in the chloroform system, were separated by the third solvent system. Each isolated component showed 98–99% purity as determined by high-performance liquid chromatography analysis. Their structures were identified by LC–MS.
Byeong Cheol Kang - One of the best experts on this subject based on the ideXlab platform.
-
Acute and subchronic toxicity of FCD, a Soybean Extract combined with L-carnitine, in Sprague-Dawley rats.
Regulatory toxicology and pharmacology : RTP, 2010Co-Authors: Jeong Hwan Che, Sang Jun Lee, Jin-ho Chung, Euna Kwon, Seung Hyun Kim, Ji Ran You, Bae Hwan Kim, Byeong Cheol KangAbstract:Abstract Soy products are primarily composed of proteins, phytochemicals such as isoflavones, soy lipids, and carbohydrates. Recently, soy isoflavones with l -carnitine were reported to exhibit anti-obesity effects in mice. FCD, a combination of Soybean Extract and l -carnitine, is a newly developed food substance. As a part of its safety assessment, acute and 13-week subchronic toxicity studies were performed in a total of 100 Sprague–Dawley (SD) rats. In the acute study, a single limit dose of 2000 mg/kg was orally administered to five male and five female rats. No adverse effects or mortality was observed during a 14-day period or upon gross pathological examination. In the subchronic study, FCD was orally administered in daily doses of 500, 1000, and 2000 mg/kg for 13 weeks, resulting in no mortality, and no changes in hematological and serum biochemistry parameters, gross pathology or histopathology. However, body weights of females were significantly decreased 10 weeks after treatment at an average of 2000 mg/kg. In addition, a slight decrease in mean food and water consumption was observed at the same dose level for 13 weeks. Therefore, the no-observed-adverse-effect-level (NOAEL) of FCD was considered to be 2000 mg/kg for male and 1000 mg/kg for female SD rats.