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L. Nakov - One of the best experts on this subject based on the ideXlab platform.
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Serum Sperm antibodies after diarrhoeal diseases
Andrologia, 2007Co-Authors: Svetoslav Kalaydjiev, D. Dimitrova, Ivan Mitov, I. Dikov, L. NakovAbstract:Summary The origin of ‘natural’ anti-Sperm antibodies found in fertile humans, virgin girls, and boys before puberty, is quite obscure. One hypothetical mechanism relates their existence to inflammatory gastrointestinal entities: as a result of the disease, cross-reactive antibodies produced against gastrointestinal flora bind Spermatozoa. To test this assumption, we evaluated the level of serum Sperm antibodies after diarrhoeal infections. Serum samples from 17 patients with shigellosis and 12 patients with salmonellosis were screened for anti-Sperm antibodies directed against Sperm surface antigens (gelatin agglutination test – GAT, tray agglutination test – TAT, Sperm immobilization test – SIT), profound Sperm antigens [enzyme-linked immunosorbent assay (ELISA)], and anti-bacterial antibodies (slide agglutination test – SAT) upon diagnosis (group A) and 4–35 days later (group B). The patients from group B demonstrated an increased Sperm Antibody incidence by GAT (20.7%), TAT (13.8%) and ELISA (31%) when compared to group A and to healthy controls, although statistically significant data were acquired only for the latter group. The absorption of positive sera with bacteria and/or Spermatozoa revealed significant reactivity changes in the Antibody values by GAT and TAT for shigellosis, and by TAT and ELISA for salmonellosis patients. These data demonstrate increased serum Sperm Antibody levels in salmonellosis and shigellosis patients.
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Serum Sperm antibodies are not elevated after mumps orchitis
Fertility and sterility, 2002Co-Authors: Svetoslav Kalaydjiev, D. Dimitrova, I. Dikov, Marina Nenova, Siika Peneva, L. NakovAbstract:Abstract Objective: To assess the level of serum Sperm antibodies after mumps orchitis. Design: Controlled descriptive study. Setting: Academic research environment. Patient(s): Seventy-four mumps orchitis patients. Intervention(s):Sampling of serum at different intervals after the onset of orchitis symptoms: 1 to 7 days, 31 to 60 days, and 61 to 431 days. Main Outcome Measure(s): Level of serum Sperm antibodies, using Kibrick's gelatin agglutination test, Friberg's tray agglutination test, Isojima's Sperm immobilization test, and ELISA. Result(s):Clinically relevant Sperm Antibody values were detected by the Friberg method among patients tested from 1 to 7 days (10.5%) and 61 to 431 days (10.5%) after the onset of disease. The Isojima test revealed a statistically insignificant higher incidence among patients at 61 to 431 days (31.6%) as compared with those sampled at 1 to 7 days (10.5%). None of the orchitis sera tested positive by the Kibrick and ELISA techniques. The established incidences did not differ significantly from the results for negative controls (blood donors) and were lower than the values acquired from positive controls (males with unexplained infertility). Conclusion(s):Mumps orchitis does not cause enhanced humoral immunity to Spermatozoa.
Ahmed Naguib Hosni - One of the best experts on this subject based on the ideXlab platform.
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Screening for Chlamydia trachomatis in Egyptian women with unexplained infertility, comparing real-time PCR techniques to standard serology tests: case control study
BMC Women's Health, 2015Co-Authors: Rana M. A. Abdella, Hatem I. Abdelmoaty, Rasha H. Elsherif, Ahmed Mahmoud Sayed, Nadine Alaa Sherif, Hisham M. Gouda, Ahmed El Lithy, Maged Almohamady, Mostafa Abdelbar, Ahmed Naguib HosniAbstract:Background To study the prevalence of Chlamydia infection in women with primary and secondary unexplained infertility using ELISA technique for Antibody detection and real time, fully automated PCR for antigen detection and to explore its association with circulating antiSperm antibodies (ASA). Methods A total of 50 women with unexplained infertility enrolled in this case control study and a control group of 44 infertile women with a known cause of infertility. Endocervical specimens were collected for Chlamydia antigen detection using PCR and serum samples for antibodies detection. Circulating anti-Sperm antibodies were detected using Sperm Antibody Latex Agglutination tests. Results The overall prevalence of Chlamydial infection in unexplained infertility cases as detected by both ELISA and PCR was 40 % (20/50). The prevalence of current Chlamydial genital infection as detected by real-time PCR was only 6.0 % (3/50); two of which were also IgM positive. Prevalence of ASA was 6.0 % (3/50); all were sero-negative for anti- C.trachomatis IgM and were PCR negative. Conclusion The incidence of Chlamydial infection in Egyptian patients with unexplained infertility is relatively high. In the setting of fertility investigations; screening for anti. C.trachomatis antibodies using ELISA, and treatment of positive cases should be considered. The presence of circulating ASA does not correlate with the presence of old or current Chlamydia infection in women with unexplained infertility.
Wang Xiao-juan - One of the best experts on this subject based on the ideXlab platform.
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Relationship between Sperm Antibody and chlamydia trachomatis and ureplasma urealyticum infections
Hainan Medical Journal, 2005Co-Authors: Wang Xiao-juanAbstract:Objective To study the relationship between Sperm Antibody and chlamydia trachomatis and ure-plasma urealyticum infections and age group,marrige of years,changes after antiinfection in infertile males,so that to asscss the causal factors in infertility and try to confirm the etiology and increase the detection and cure rate.Methods Sperm Antibody in94male infertility patients with CT and UU infections and62normal productive men were tested by Mixed antiglobulin reaction test.Results The AsAb in CT and UU positive and normal groups were35.1%,4.8%,the differences between CT/UU positive and normal groups were significant(P0.01),after antiinfection,9AaAb positive patiebts became negative,the longer the marrige of years were,the higher the Sperm Antibody positive rate were,to become negative was lower after treat.Conclusions There was very close relationship between Sperm Antibody and chlamydia trachomatis and ureplasma urealyticum infections,it was an important factor in male immune infertility.
Svetoslav Kalaydjiev - One of the best experts on this subject based on the ideXlab platform.
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Serum Sperm antibodies after diarrhoeal diseases
Andrologia, 2007Co-Authors: Svetoslav Kalaydjiev, D. Dimitrova, Ivan Mitov, I. Dikov, L. NakovAbstract:Summary The origin of ‘natural’ anti-Sperm antibodies found in fertile humans, virgin girls, and boys before puberty, is quite obscure. One hypothetical mechanism relates their existence to inflammatory gastrointestinal entities: as a result of the disease, cross-reactive antibodies produced against gastrointestinal flora bind Spermatozoa. To test this assumption, we evaluated the level of serum Sperm antibodies after diarrhoeal infections. Serum samples from 17 patients with shigellosis and 12 patients with salmonellosis were screened for anti-Sperm antibodies directed against Sperm surface antigens (gelatin agglutination test – GAT, tray agglutination test – TAT, Sperm immobilization test – SIT), profound Sperm antigens [enzyme-linked immunosorbent assay (ELISA)], and anti-bacterial antibodies (slide agglutination test – SAT) upon diagnosis (group A) and 4–35 days later (group B). The patients from group B demonstrated an increased Sperm Antibody incidence by GAT (20.7%), TAT (13.8%) and ELISA (31%) when compared to group A and to healthy controls, although statistically significant data were acquired only for the latter group. The absorption of positive sera with bacteria and/or Spermatozoa revealed significant reactivity changes in the Antibody values by GAT and TAT for shigellosis, and by TAT and ELISA for salmonellosis patients. These data demonstrate increased serum Sperm Antibody levels in salmonellosis and shigellosis patients.
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Serum Sperm antibodies are not elevated after mumps orchitis
Fertility and sterility, 2002Co-Authors: Svetoslav Kalaydjiev, D. Dimitrova, I. Dikov, Marina Nenova, Siika Peneva, L. NakovAbstract:Abstract Objective: To assess the level of serum Sperm antibodies after mumps orchitis. Design: Controlled descriptive study. Setting: Academic research environment. Patient(s): Seventy-four mumps orchitis patients. Intervention(s):Sampling of serum at different intervals after the onset of orchitis symptoms: 1 to 7 days, 31 to 60 days, and 61 to 431 days. Main Outcome Measure(s): Level of serum Sperm antibodies, using Kibrick's gelatin agglutination test, Friberg's tray agglutination test, Isojima's Sperm immobilization test, and ELISA. Result(s):Clinically relevant Sperm Antibody values were detected by the Friberg method among patients tested from 1 to 7 days (10.5%) and 61 to 431 days (10.5%) after the onset of disease. The Isojima test revealed a statistically insignificant higher incidence among patients at 61 to 431 days (31.6%) as compared with those sampled at 1 to 7 days (10.5%). None of the orchitis sera tested positive by the Kibrick and ELISA techniques. The established incidences did not differ significantly from the results for negative controls (blood donors) and were lower than the values acquired from positive controls (males with unexplained infertility). Conclusion(s):Mumps orchitis does not cause enhanced humoral immunity to Spermatozoa.
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Serum Sperm antibodies unrelated to mumps orchitis.
Andrologia, 2001Co-Authors: Svetoslav Kalaydjiev, D. Dimitrova, P. Tsvetkova, D. TsvetkovAbstract:In order to determine whether there is an association between mumps orchitis and serum Sperm antibodies, we tested patients at the time orchitis was diagnosed (n=7) and individuals who had had orchitis at least 1 month previously (n=14). Data were compared with the results for a control group of blood donors (n=20). Sperm antibodies were detected by the gelatin agglutination test of Kibrick, the tray agglutination test of Friberg and the ELISA. Clinically significant Sperm Antibody levels were not found in patients in the early stages of the disease. Four subjects tested post-disease were positive in the Friberg test and one was positive in the ELISA. One control serum was also positive in the latter test. Significant differences were not found between levels in patients in the early stages of the disease and levels in individuals post-disease, although the results of the Friberg test differed significantly between controls and former mumps orchitis cases. These data do not support the assumption of an involvement of humoral immunity against Spermatozoa in mumps orchitis patients.
P L Matson - One of the best experts on this subject based on the ideXlab platform.
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andrology external quality assessment for semen analysis and Sperm Antibody detection results of a pilot scheme
Human Reproduction, 1995Co-Authors: P L MatsonAbstract:The need for quality assurance in the seminology laboratory is clear, as the techniques of semen analysis and Sperm Antibody detection are just as susceptible to variation as any other routine pathology test. Semen samples were distributed to 20 laboratories on six occasions, four samples per distribution, for Sperm concentration and morphology assessment under routine conditions, together with an equal number of serum samples for Sperm Antibody detection. The semen analysis results showed a wide range of values for any given sample, which did not seem to be related to the methodology used. However, this variation appears to be related to the presence of persistent errors, as most laboratories showed reasonable between-assay and within-assay variation. Detection of Sperm antibodies by the tray agglutination, gelatin agglutination or indirect immunobead test showed a consistent discrimination between the intended positive and intended negative samples. However, the use of fluorescent microscopy was unable to do this. This study has shown the feasibility of operating external quality assessment schemes for semen analysis and Sperm Antibody detection. These schemes provide the opportunity for individual laboratories to fully evaluate their own methods against those of others and to determine the stages at which any errors occur. An increased number of participants will ultimately enable a systematic comparison of different methods.