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Leah Yogev - One of the best experts on this subject based on the ideXlab platform.
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Freezability and Semen Parameters in Candidates of Sperm Bank Donors: 1992-2010
Journal of andrology, 2012Co-Authors: Leah Yogev, Haim Yavetz, Sandra E. Kleiman, Amnon Botchan, Gedalia Paz, Esther Shabtai, Ronni Gamzu, Ofer Lehavi, Ron HauserAbstract:There has been considerable concern worldwide about possible semen quality deterioration over the last 2 decades. The aim of this study was to evaluate freezability and semen quality of healthy young males during the years 1992-2010. A total of 1211 young (20-32 years old) candidates for Sperm Bank donation were recruited into the study with no exclusion criteria. They were instructed to observe 2 to 3 days of abstinence from sexual activity, and most of them supplied 2 specimens each. Average values of the various semen parameters, including freezing survival, were calculated for each participant. The change in different semen parameters over years, according to yearly and monthly average temperatures, was evaluated by SAS PROC SURVEYREG analysis. During that period, there were significant increases in motility and vitality percentages, as well as in the percentage of thawed Sperm motility. The parameters of volume, concentration, normal morphology, total count, and total motile count showed a significant decrease with years (P < .01). The significant increase in average yearly temperature (P < .004) had limited, nonsignificant association with any of the semen variables. However, average monthly temperature contributed significantly to the trend of semen quality parameters (ie, specimen volume, concentration, percentage of normal morphology, and thawed motility). To the best of our knowledge, this is the first demonstration of the occurrence of an improvement in percent thawed motility over the years, and its significance lies in enabling a higher proportion of Sperm Bank candidates to be suitable for donation. It is suggested that the global warming phenomenon might have only partial contribution to semen variable changes over the years.
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Long-term cryostorage of Sperm in a human Sperm Bank does not damage progressive motility concentration
Human reproduction (Oxford England), 2010Co-Authors: Leah Yogev, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Esther Shabtai, Ofer Lehavi, Ronni GamzuAbstract:background: The use of quarantined cryopreserved semen is mandatory in donor insemination programs. Whether Sperm cells can survive and retain their ability to fertilize after long-term storage remains a controversial issue. The objective of this study was to determine the effect of the duration of cryostorage in liquid nitrogen on the Sperm cells’ progressive motility concentration (PMC) in a large study group. methods: A total of 2525 thawed Sperm specimens, packed in straws and donated by 72 Sperm Bank donors for intrauterine insemination (IUI), were evaluated in an assisted reproduction institute. PMC was recorded after 0.5 –14.4 years of cryostorage. results: The mean (+SD) value of PMC of all study samples was 10.8+ 3.3 10 6 /ml after freezing/thawing and before cryostorage (T0), and 12.3+ 2.9 10 6 /ml after storage and before using the specimen for IUI (T1, P , 0.0001). Specimen storage for different lengths of time revealed that storage duration had no significant influence on the PMC of the specimens (r ¼ 20.03, P ¼ 0.08). The PMC of partially filled straws was lower than in full straws. Cryostorage duration made no difference in the PMC of raw and washed Sperm specimens. conclusion: Prolonged storage of donated Sperm in liquid nitrogen had no influence on the PMC of the specimens and therefore should not alter the fertilization potency of donated Sperm. The high post-storage values of the PMC compared with the pre-storage PMC values was probably an artifact of the small volume of the pre-storage sample.
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Effect of long-term storage on deoxyribonucleic acid damage and motility of Sperm Bank donor specimens.
Fertility and sterility, 2007Co-Authors: Avital Edelstein, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Leah YogevAbstract:The percentage of Sperm DNA damage in samples from Sperm Bank donors was not significantly different ( P =.17), whereas the percentage of motile cells was lower ( P =.009) after long-term (9–13 years) compared with short-term (1–5 years) storage. Density gradient isolation reduced the difference in Sperm motility between the two groups.
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Deoxyribonucleic acid-damaged Sperm in cryopreserved-thawed specimens from cancer patients and healthy men
Fertility and sterility, 2007Co-Authors: Avital Edelstein, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Leah YogevAbstract:A similarity was found between the percentage of thawed, DNA-damaged Spermatozoa in cancer patients and that in candidates to become Sperm Bank donors who had low Sperm cryofreezability. Both groups were significantly different from the Sperm Bank donor group. It is suggested that the higher rate of DNA fragmentation in Sperm from cancer patients compared with Sperm Bank donors is apparently a result of selecting donors by the level of Sperm cryofreezability (i.e., high), rather than a direct effect of an existing malignancy.
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Hemizona assay for evaluating fertilizing capacity of human Spermatozoa
Harefuah, 1993Co-Authors: Ronni Gamzu, Haim Yavetz, Leah Yogev, Zvi T. Homonnai, Gedalia PazAbstract:The hemizona assay (HZA), which tests the binding capacity of human Spermatozoa to the zona pellucida, is described. This assay has very high sensitivity (86-100%) in predicting fertilizing capacity in the in vitro fertilization (IVF) system. It is recommended that it be carried out on semen samples before entering the IVF program. In addition, it can also help evaluate unexplained infertility and assess the quality of ova. The necessity for a control fertilized sample in each HZA might complicate the assay. To simplify the test, the use of Sperm from the Sperm Bank was investigated. It was shown that fresh and frozen-thawed Sperm have the same capacity to bind to the hemizonae. Thus, frozen donor semen preserved in the Sperm Bank can be used for controlling the HZA.
Igael Madgar - One of the best experts on this subject based on the ideXlab platform.
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a child of hers older single mothers and their children conceived through ivf with both egg and Sperm donation
Fertility and Sterility, 2008Co-Authors: Ruth Landau, R Weissenberg, Igael MadgarAbstract:Objective To study the decision to have a child alone, the experience of gamete donation, the issue of disclosure of the donor link to the child, conception-related health and sociodemographic characteristics of the mothers, children's socioemotional development, and mother-child relationships. Design Qualitative study. Setting Prior clients of a Sperm Bank. Participant(s) Eleven single women in their late forties who gave birth to children aided by IVF involving both egg and Sperm donation, i.e., the children are not genetically related to the mothers. Result(s) Not only were there differences among the participants, but they also differed from the only previous study focusing on single women becoming mothers by choice and using advanced reproductive technologies. Conclusion(s) Similarly to previous studies, we generally found that the impact of assisted conception on parenting and child development gives no undue cause for concern while the children are still young. However, the young age of the children in our sample prevented us from answering many questions about the children's socioemotional development and about disclosure of donor conception to children born to older single women using double gamete donation and IVF.
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A child of “hers”: older single mothers and their children conceived through IVF with both egg and Sperm donation
Fertility and Sterility, 2007Co-Authors: Ruth Landau, R Weissenberg, Igael MadgarAbstract:Objective To study the decision to have a child alone, the experience of gamete donation, the issue of disclosure of the donor link to the child, conception-related health and sociodemographic characteristics of the mothers, children's socioemotional development, and mother-child relationships. Design Qualitative study. Setting Prior clients of a Sperm Bank. Participant(s) Eleven single women in their late forties who gave birth to children aided by IVF involving both egg and Sperm donation, i.e., the children are not genetically related to the mothers. Result(s) Not only were there differences among the participants, but they also differed from the only previous study focusing on single women becoming mothers by choice and using advanced reproductive technologies. Conclusion(s) Similarly to previous studies, we generally found that the impact of assisted conception on parenting and child development gives no undue cause for concern while the children are still young. However, the young age of the children in our sample prevented us from answering many questions about the children's socioemotional development and about disclosure of donor conception to children born to older single women using double gamete donation and IVF.
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older single mothers assisted by Sperm donation and their children
Human Reproduction, 2007Co-Authors: R Weissenberg, Ruth Landau, Igael MadgarAbstract:BACKGROUND: Knowledge about the lives of single women who choose to become mothers by Sperm donation is very limited. METHODS: This study comprises 62 families headed by formally single women who, following their decision to give birth to a child with the aid of Sperm donation, by means of insemination or in vitro fertilization (IVF), used the services of one Sperm Bank in Israel. RESULTS: The findings of the study, based on the reports obtained from the mothers in face-to-face interviews by structured questionnaires with closed-ended scales and single item open questions, present a complex picture of formally single-mother families assisted by Sperm donation. They shed light on socio-demographic and conception related information of the mothers in the sample, on mothers' and children's health, on the children's socio-emotional development and mother-child relationship and on the mothers' difficulties and needs encountered in their function as single parents. CONCLUSIONS: Although the currently young children's socio-emotional development seems to be within the normal range, the mean age of 43 years at first birth of the mothers, the fact that about one-fifth of them gave birth to twins, the health condition of some of the mothers and children, and the difficulties they encounter, may raise some concerns.
Ron Hauser - One of the best experts on this subject based on the ideXlab platform.
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Freezability and Semen Parameters in Candidates of Sperm Bank Donors: 1992-2010
Journal of andrology, 2012Co-Authors: Leah Yogev, Haim Yavetz, Sandra E. Kleiman, Amnon Botchan, Gedalia Paz, Esther Shabtai, Ronni Gamzu, Ofer Lehavi, Ron HauserAbstract:There has been considerable concern worldwide about possible semen quality deterioration over the last 2 decades. The aim of this study was to evaluate freezability and semen quality of healthy young males during the years 1992-2010. A total of 1211 young (20-32 years old) candidates for Sperm Bank donation were recruited into the study with no exclusion criteria. They were instructed to observe 2 to 3 days of abstinence from sexual activity, and most of them supplied 2 specimens each. Average values of the various semen parameters, including freezing survival, were calculated for each participant. The change in different semen parameters over years, according to yearly and monthly average temperatures, was evaluated by SAS PROC SURVEYREG analysis. During that period, there were significant increases in motility and vitality percentages, as well as in the percentage of thawed Sperm motility. The parameters of volume, concentration, normal morphology, total count, and total motile count showed a significant decrease with years (P < .01). The significant increase in average yearly temperature (P < .004) had limited, nonsignificant association with any of the semen variables. However, average monthly temperature contributed significantly to the trend of semen quality parameters (ie, specimen volume, concentration, percentage of normal morphology, and thawed motility). To the best of our knowledge, this is the first demonstration of the occurrence of an improvement in percent thawed motility over the years, and its significance lies in enabling a higher proportion of Sperm Bank candidates to be suitable for donation. It is suggested that the global warming phenomenon might have only partial contribution to semen variable changes over the years.
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Long-term cryostorage of Sperm in a human Sperm Bank does not damage progressive motility concentration
Human reproduction (Oxford England), 2010Co-Authors: Leah Yogev, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Esther Shabtai, Ofer Lehavi, Ronni GamzuAbstract:background: The use of quarantined cryopreserved semen is mandatory in donor insemination programs. Whether Sperm cells can survive and retain their ability to fertilize after long-term storage remains a controversial issue. The objective of this study was to determine the effect of the duration of cryostorage in liquid nitrogen on the Sperm cells’ progressive motility concentration (PMC) in a large study group. methods: A total of 2525 thawed Sperm specimens, packed in straws and donated by 72 Sperm Bank donors for intrauterine insemination (IUI), were evaluated in an assisted reproduction institute. PMC was recorded after 0.5 –14.4 years of cryostorage. results: The mean (+SD) value of PMC of all study samples was 10.8+ 3.3 10 6 /ml after freezing/thawing and before cryostorage (T0), and 12.3+ 2.9 10 6 /ml after storage and before using the specimen for IUI (T1, P , 0.0001). Specimen storage for different lengths of time revealed that storage duration had no significant influence on the PMC of the specimens (r ¼ 20.03, P ¼ 0.08). The PMC of partially filled straws was lower than in full straws. Cryostorage duration made no difference in the PMC of raw and washed Sperm specimens. conclusion: Prolonged storage of donated Sperm in liquid nitrogen had no influence on the PMC of the specimens and therefore should not alter the fertilization potency of donated Sperm. The high post-storage values of the PMC compared with the pre-storage PMC values was probably an artifact of the small volume of the pre-storage sample.
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Effect of long-term storage on deoxyribonucleic acid damage and motility of Sperm Bank donor specimens.
Fertility and sterility, 2007Co-Authors: Avital Edelstein, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Leah YogevAbstract:The percentage of Sperm DNA damage in samples from Sperm Bank donors was not significantly different ( P =.17), whereas the percentage of motile cells was lower ( P =.009) after long-term (9–13 years) compared with short-term (1–5 years) storage. Density gradient isolation reduced the difference in Sperm motility between the two groups.
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Deoxyribonucleic acid-damaged Sperm in cryopreserved-thawed specimens from cancer patients and healthy men
Fertility and sterility, 2007Co-Authors: Avital Edelstein, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Leah YogevAbstract:A similarity was found between the percentage of thawed, DNA-damaged Spermatozoa in cancer patients and that in candidates to become Sperm Bank donors who had low Sperm cryofreezability. Both groups were significantly different from the Sperm Bank donor group. It is suggested that the higher rate of DNA fragmentation in Sperm from cancer patients compared with Sperm Bank donors is apparently a result of selecting donors by the level of Sperm cryofreezability (i.e., high), rather than a direct effect of an existing malignancy.
Haim Yavetz - One of the best experts on this subject based on the ideXlab platform.
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Freezability and Semen Parameters in Candidates of Sperm Bank Donors: 1992-2010
Journal of andrology, 2012Co-Authors: Leah Yogev, Haim Yavetz, Sandra E. Kleiman, Amnon Botchan, Gedalia Paz, Esther Shabtai, Ronni Gamzu, Ofer Lehavi, Ron HauserAbstract:There has been considerable concern worldwide about possible semen quality deterioration over the last 2 decades. The aim of this study was to evaluate freezability and semen quality of healthy young males during the years 1992-2010. A total of 1211 young (20-32 years old) candidates for Sperm Bank donation were recruited into the study with no exclusion criteria. They were instructed to observe 2 to 3 days of abstinence from sexual activity, and most of them supplied 2 specimens each. Average values of the various semen parameters, including freezing survival, were calculated for each participant. The change in different semen parameters over years, according to yearly and monthly average temperatures, was evaluated by SAS PROC SURVEYREG analysis. During that period, there were significant increases in motility and vitality percentages, as well as in the percentage of thawed Sperm motility. The parameters of volume, concentration, normal morphology, total count, and total motile count showed a significant decrease with years (P < .01). The significant increase in average yearly temperature (P < .004) had limited, nonsignificant association with any of the semen variables. However, average monthly temperature contributed significantly to the trend of semen quality parameters (ie, specimen volume, concentration, percentage of normal morphology, and thawed motility). To the best of our knowledge, this is the first demonstration of the occurrence of an improvement in percent thawed motility over the years, and its significance lies in enabling a higher proportion of Sperm Bank candidates to be suitable for donation. It is suggested that the global warming phenomenon might have only partial contribution to semen variable changes over the years.
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Long-term cryostorage of Sperm in a human Sperm Bank does not damage progressive motility concentration
Human reproduction (Oxford England), 2010Co-Authors: Leah Yogev, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Esther Shabtai, Ofer Lehavi, Ronni GamzuAbstract:background: The use of quarantined cryopreserved semen is mandatory in donor insemination programs. Whether Sperm cells can survive and retain their ability to fertilize after long-term storage remains a controversial issue. The objective of this study was to determine the effect of the duration of cryostorage in liquid nitrogen on the Sperm cells’ progressive motility concentration (PMC) in a large study group. methods: A total of 2525 thawed Sperm specimens, packed in straws and donated by 72 Sperm Bank donors for intrauterine insemination (IUI), were evaluated in an assisted reproduction institute. PMC was recorded after 0.5 –14.4 years of cryostorage. results: The mean (+SD) value of PMC of all study samples was 10.8+ 3.3 10 6 /ml after freezing/thawing and before cryostorage (T0), and 12.3+ 2.9 10 6 /ml after storage and before using the specimen for IUI (T1, P , 0.0001). Specimen storage for different lengths of time revealed that storage duration had no significant influence on the PMC of the specimens (r ¼ 20.03, P ¼ 0.08). The PMC of partially filled straws was lower than in full straws. Cryostorage duration made no difference in the PMC of raw and washed Sperm specimens. conclusion: Prolonged storage of donated Sperm in liquid nitrogen had no influence on the PMC of the specimens and therefore should not alter the fertilization potency of donated Sperm. The high post-storage values of the PMC compared with the pre-storage PMC values was probably an artifact of the small volume of the pre-storage sample.
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Effect of long-term storage on deoxyribonucleic acid damage and motility of Sperm Bank donor specimens.
Fertility and sterility, 2007Co-Authors: Avital Edelstein, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Leah YogevAbstract:The percentage of Sperm DNA damage in samples from Sperm Bank donors was not significantly different ( P =.17), whereas the percentage of motile cells was lower ( P =.009) after long-term (9–13 years) compared with short-term (1–5 years) storage. Density gradient isolation reduced the difference in Sperm motility between the two groups.
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Deoxyribonucleic acid-damaged Sperm in cryopreserved-thawed specimens from cancer patients and healthy men
Fertility and sterility, 2007Co-Authors: Avital Edelstein, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Leah YogevAbstract:A similarity was found between the percentage of thawed, DNA-damaged Spermatozoa in cancer patients and that in candidates to become Sperm Bank donors who had low Sperm cryofreezability. Both groups were significantly different from the Sperm Bank donor group. It is suggested that the higher rate of DNA fragmentation in Sperm from cancer patients compared with Sperm Bank donors is apparently a result of selecting donors by the level of Sperm cryofreezability (i.e., high), rather than a direct effect of an existing malignancy.
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Hemizona assay for evaluating fertilizing capacity of human Spermatozoa
Harefuah, 1993Co-Authors: Ronni Gamzu, Haim Yavetz, Leah Yogev, Zvi T. Homonnai, Gedalia PazAbstract:The hemizona assay (HZA), which tests the binding capacity of human Spermatozoa to the zona pellucida, is described. This assay has very high sensitivity (86-100%) in predicting fertilizing capacity in the in vitro fertilization (IVF) system. It is recommended that it be carried out on semen samples before entering the IVF program. In addition, it can also help evaluate unexplained infertility and assess the quality of ova. The necessity for a control fertilized sample in each HZA might complicate the assay. To simplify the test, the use of Sperm from the Sperm Bank was investigated. It was shown that fresh and frozen-thawed Sperm have the same capacity to bind to the hemizonae. Thus, frozen donor semen preserved in the Sperm Bank can be used for controlling the HZA.
Sandra E. Kleiman - One of the best experts on this subject based on the ideXlab platform.
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Freezability and Semen Parameters in Candidates of Sperm Bank Donors: 1992-2010
Journal of andrology, 2012Co-Authors: Leah Yogev, Haim Yavetz, Sandra E. Kleiman, Amnon Botchan, Gedalia Paz, Esther Shabtai, Ronni Gamzu, Ofer Lehavi, Ron HauserAbstract:There has been considerable concern worldwide about possible semen quality deterioration over the last 2 decades. The aim of this study was to evaluate freezability and semen quality of healthy young males during the years 1992-2010. A total of 1211 young (20-32 years old) candidates for Sperm Bank donation were recruited into the study with no exclusion criteria. They were instructed to observe 2 to 3 days of abstinence from sexual activity, and most of them supplied 2 specimens each. Average values of the various semen parameters, including freezing survival, were calculated for each participant. The change in different semen parameters over years, according to yearly and monthly average temperatures, was evaluated by SAS PROC SURVEYREG analysis. During that period, there were significant increases in motility and vitality percentages, as well as in the percentage of thawed Sperm motility. The parameters of volume, concentration, normal morphology, total count, and total motile count showed a significant decrease with years (P < .01). The significant increase in average yearly temperature (P < .004) had limited, nonsignificant association with any of the semen variables. However, average monthly temperature contributed significantly to the trend of semen quality parameters (ie, specimen volume, concentration, percentage of normal morphology, and thawed motility). To the best of our knowledge, this is the first demonstration of the occurrence of an improvement in percent thawed motility over the years, and its significance lies in enabling a higher proportion of Sperm Bank candidates to be suitable for donation. It is suggested that the global warming phenomenon might have only partial contribution to semen variable changes over the years.
-
Long-term cryostorage of Sperm in a human Sperm Bank does not damage progressive motility concentration
Human reproduction (Oxford England), 2010Co-Authors: Leah Yogev, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Esther Shabtai, Ofer Lehavi, Ronni GamzuAbstract:background: The use of quarantined cryopreserved semen is mandatory in donor insemination programs. Whether Sperm cells can survive and retain their ability to fertilize after long-term storage remains a controversial issue. The objective of this study was to determine the effect of the duration of cryostorage in liquid nitrogen on the Sperm cells’ progressive motility concentration (PMC) in a large study group. methods: A total of 2525 thawed Sperm specimens, packed in straws and donated by 72 Sperm Bank donors for intrauterine insemination (IUI), were evaluated in an assisted reproduction institute. PMC was recorded after 0.5 –14.4 years of cryostorage. results: The mean (+SD) value of PMC of all study samples was 10.8+ 3.3 10 6 /ml after freezing/thawing and before cryostorage (T0), and 12.3+ 2.9 10 6 /ml after storage and before using the specimen for IUI (T1, P , 0.0001). Specimen storage for different lengths of time revealed that storage duration had no significant influence on the PMC of the specimens (r ¼ 20.03, P ¼ 0.08). The PMC of partially filled straws was lower than in full straws. Cryostorage duration made no difference in the PMC of raw and washed Sperm specimens. conclusion: Prolonged storage of donated Sperm in liquid nitrogen had no influence on the PMC of the specimens and therefore should not alter the fertilization potency of donated Sperm. The high post-storage values of the PMC compared with the pre-storage PMC values was probably an artifact of the small volume of the pre-storage sample.
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Effect of long-term storage on deoxyribonucleic acid damage and motility of Sperm Bank donor specimens.
Fertility and sterility, 2007Co-Authors: Avital Edelstein, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Leah YogevAbstract:The percentage of Sperm DNA damage in samples from Sperm Bank donors was not significantly different ( P =.17), whereas the percentage of motile cells was lower ( P =.009) after long-term (9–13 years) compared with short-term (1–5 years) storage. Density gradient isolation reduced the difference in Sperm motility between the two groups.
-
Deoxyribonucleic acid-damaged Sperm in cryopreserved-thawed specimens from cancer patients and healthy men
Fertility and sterility, 2007Co-Authors: Avital Edelstein, Haim Yavetz, Sandra E. Kleiman, Ron Hauser, Amnon Botchan, Gedalia Paz, Leah YogevAbstract:A similarity was found between the percentage of thawed, DNA-damaged Spermatozoa in cancer patients and that in candidates to become Sperm Bank donors who had low Sperm cryofreezability. Both groups were significantly different from the Sperm Bank donor group. It is suggested that the higher rate of DNA fragmentation in Sperm from cancer patients compared with Sperm Bank donors is apparently a result of selecting donors by the level of Sperm cryofreezability (i.e., high), rather than a direct effect of an existing malignancy.