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Ch. V. Ramana - One of the best experts on this subject based on the ideXlab platform.

  • Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as n
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    An obligately anaerobic Spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( 

  • alkalispirochaeta cellulosivorans gen nov sp nov a cellulose hydrolysing alkaliphilic halotolerant bacterium isolated from the gut of a wood eating cockroach cryptocercus punctulatus and reclassification of four species of spirochaeta as new combinat
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    An obligately anaerobic Spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( < 92.7 %) as its closest phylogenetic neighbours. However, DNA–DNA hybridization between strain JC227T and S. sphaeroplastigenens JC133T, S. odontotermitis JC202T, S. alkalica DSM 8900T and S. americana DSM 14872T was 62 ± 2, 63, 58 ± 2 and 48 ± 4 %, respectively. Strain JC227T contained phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and six unidentified lipids. Summed feature C18 : 1ω7c/C18 : 1ω6c was the predominant cellular fatty acid, with significant proportions of C16 : 0, C14 : 0, C12 : 0, C15 : 1ω6c, C16 : 1ω5c, C16 : 1ω6c/C16 : 1ω7c and C17 : 0 2-OH. The DNA G+C content of strain JC227T was 55.5 mol%. On the basis of physiological, biochemical, chemotaxonomic (including metabolomic) and genomic differences from previously described taxa, strain JC227T can be differentiated from members of the genus Spirochaeta and represents a novel species of a new genus, for which the name Alkalispirochaeta cellulosivorans gen. nov., sp. nov. is proposed. The type strain of Alkalispirochaeta cellulosivorans is JC227T ( = KCTC 15343T = NBRC 110105T). We also propose the reclassification of Spirochaeta sphaeroplastigenens, Spirochaeta odontotermitis, Spirochaeta alkalica and Spirochaeta americana as Alkalispirochaeta sphaeroplastigenens comb. nov. (type strain JC133T = KCTC 15220T = NBRC 109056T), Alkalispirochaeta odontotermitis comb. nov. (type strain JC202T = KCTC 15324T = NBRC 110104T), Alkalispirochaeta alkalica comb. nov. (type strain Z-7491T = DSM 8900T = ATCC 700262T) and Alkalispirochaeta americana comb. nov. (type strain ASpG1T = ATCC BAA-392T = DSM 14872T). The type species of Alkalispirochaeta gen. nov. is Alkalispirochaeta alkalica comb. nov.

  • Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic Spirochaete isolated from the termite Odontotermes obesus (Rambur) gut
    International Journal of Systematic and Evolutionary Microbiology, 2015
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    A Gram-stain-negative Spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( < 93.83 %) as the closest phylogenetic neighbours. However, mean DNA–DNA hydridization values between strain JC202T and S. sphaeroplastigenens JC133T, S. alkalica DSM 8900T ( = Z-7491T) and S. americana DSM 14872T ( = ASpG1T) were 55 ± 2, 22 ± 3 and 32 ± 1 %, respectively. On the basis of physiological, biochemical, chemotaxonomic (including metabolome) and genomic differences from the previously described taxa, strain JC202T is differentiated from other members of the genus Spirochaeta and is considered to represent a novel species, for which the name Spirochaeta odontotermitis sp. nov. is proposed. The type strain is JC202T ( = KCTC 15324T = NBRC 110104T).

  • Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic Spirochaete isolated from the termite Odontotermes obesus (Rambur) gut.
    International journal of systematic and evolutionary microbiology, 2015
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    A Gram-stain-negative Spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( 

  • Spirochaeta lutea sp. nov., isolated from marine habitats and emended description of the genus Spirochaeta.
    Systematic and Applied Microbiology, 2015
    Co-Authors: Y. Shivani, L. Tushar, Ch. Sasikala, Y Subhash, Ch. V. Ramana
    Abstract:

    Abstract Metagenome analysis of forty samples indicated a rich diversity of free living Spirochaetes among Indian habitats. Only 2–3% of the total bacterial diversity was represented by Spirochaetes with five distinct phylogenetic OTUs. A strain (JC230 T ) was brought to cultivation and its 16S rRNA gene sequence showed highest sequence similarity with that of Spirochaeta asiatica Z-7591 T (90.78%). Genome sequence analysis of strain JC230 T indicates the presence of 117 genes related to motility and chemotaxis. Strain JC230 T is an obligate anaerobe. It is helical shaped, Gram-stain-negative and catalase and oxidase negative. Strain JC230 T produces carotenoids and have all the genes related to carotenogenesis of spirilloxanthin series. Isoprenoid biosynthesis occurs through HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) pathway. Twelve bacterial haemoglobin and one flavohaemoglobin related genes are present in the genome of strain JC230 T . The genome of strain JC230 T showed the presence of heavy metal resistance related genes of cobalt, zinc, cadmium and arsenic. Six genes related to colicin V and bacteriocin production cluster are present. Major (>2%) fatty acids were C 12:0 , C 14:0 , iso -C 14:0 , iso -C 15:1 H/C 13:0 3OH, anteiso -C 15:0 , C 16:0 , and iso -C 16:0 . Strain JC230 T contains diphosphatylglycerol, phosphatydilglycerol, phosphatydilethanolamine, unidentified phospholipid (PL1) and unidentified lipids (L1-7). G + C mol% of strain JC230 T was 54.1%. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC230 T as a representative of a new species in the genus Spirochaeta , for which the name Spirochaeta lutea sp. nov. is proposed. The type strain is JC230 T (=KCTC 15387 T  = DSM 29074 T ).

T. Sravanthi - One of the best experts on this subject based on the ideXlab platform.

  • Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as n
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    An obligately anaerobic Spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( 

  • alkalispirochaeta cellulosivorans gen nov sp nov a cellulose hydrolysing alkaliphilic halotolerant bacterium isolated from the gut of a wood eating cockroach cryptocercus punctulatus and reclassification of four species of spirochaeta as new combinat
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    An obligately anaerobic Spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( < 92.7 %) as its closest phylogenetic neighbours. However, DNA–DNA hybridization between strain JC227T and S. sphaeroplastigenens JC133T, S. odontotermitis JC202T, S. alkalica DSM 8900T and S. americana DSM 14872T was 62 ± 2, 63, 58 ± 2 and 48 ± 4 %, respectively. Strain JC227T contained phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and six unidentified lipids. Summed feature C18 : 1ω7c/C18 : 1ω6c was the predominant cellular fatty acid, with significant proportions of C16 : 0, C14 : 0, C12 : 0, C15 : 1ω6c, C16 : 1ω5c, C16 : 1ω6c/C16 : 1ω7c and C17 : 0 2-OH. The DNA G+C content of strain JC227T was 55.5 mol%. On the basis of physiological, biochemical, chemotaxonomic (including metabolomic) and genomic differences from previously described taxa, strain JC227T can be differentiated from members of the genus Spirochaeta and represents a novel species of a new genus, for which the name Alkalispirochaeta cellulosivorans gen. nov., sp. nov. is proposed. The type strain of Alkalispirochaeta cellulosivorans is JC227T ( = KCTC 15343T = NBRC 110105T). We also propose the reclassification of Spirochaeta sphaeroplastigenens, Spirochaeta odontotermitis, Spirochaeta alkalica and Spirochaeta americana as Alkalispirochaeta sphaeroplastigenens comb. nov. (type strain JC133T = KCTC 15220T = NBRC 109056T), Alkalispirochaeta odontotermitis comb. nov. (type strain JC202T = KCTC 15324T = NBRC 110104T), Alkalispirochaeta alkalica comb. nov. (type strain Z-7491T = DSM 8900T = ATCC 700262T) and Alkalispirochaeta americana comb. nov. (type strain ASpG1T = ATCC BAA-392T = DSM 14872T). The type species of Alkalispirochaeta gen. nov. is Alkalispirochaeta alkalica comb. nov.

  • Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic Spirochaete isolated from the termite Odontotermes obesus (Rambur) gut
    International Journal of Systematic and Evolutionary Microbiology, 2015
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    A Gram-stain-negative Spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( < 93.83 %) as the closest phylogenetic neighbours. However, mean DNA–DNA hydridization values between strain JC202T and S. sphaeroplastigenens JC133T, S. alkalica DSM 8900T ( = Z-7491T) and S. americana DSM 14872T ( = ASpG1T) were 55 ± 2, 22 ± 3 and 32 ± 1 %, respectively. On the basis of physiological, biochemical, chemotaxonomic (including metabolome) and genomic differences from the previously described taxa, strain JC202T is differentiated from other members of the genus Spirochaeta and is considered to represent a novel species, for which the name Spirochaeta odontotermitis sp. nov. is proposed. The type strain is JC202T ( = KCTC 15324T = NBRC 110104T).

  • Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic Spirochaete isolated from the termite Odontotermes obesus (Rambur) gut.
    International journal of systematic and evolutionary microbiology, 2015
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    A Gram-stain-negative Spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( 

Ch. Sasikala - One of the best experts on this subject based on the ideXlab platform.

  • Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as n
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    An obligately anaerobic Spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( 

  • alkalispirochaeta cellulosivorans gen nov sp nov a cellulose hydrolysing alkaliphilic halotolerant bacterium isolated from the gut of a wood eating cockroach cryptocercus punctulatus and reclassification of four species of spirochaeta as new combinat
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    An obligately anaerobic Spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( < 92.7 %) as its closest phylogenetic neighbours. However, DNA–DNA hybridization between strain JC227T and S. sphaeroplastigenens JC133T, S. odontotermitis JC202T, S. alkalica DSM 8900T and S. americana DSM 14872T was 62 ± 2, 63, 58 ± 2 and 48 ± 4 %, respectively. Strain JC227T contained phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and six unidentified lipids. Summed feature C18 : 1ω7c/C18 : 1ω6c was the predominant cellular fatty acid, with significant proportions of C16 : 0, C14 : 0, C12 : 0, C15 : 1ω6c, C16 : 1ω5c, C16 : 1ω6c/C16 : 1ω7c and C17 : 0 2-OH. The DNA G+C content of strain JC227T was 55.5 mol%. On the basis of physiological, biochemical, chemotaxonomic (including metabolomic) and genomic differences from previously described taxa, strain JC227T can be differentiated from members of the genus Spirochaeta and represents a novel species of a new genus, for which the name Alkalispirochaeta cellulosivorans gen. nov., sp. nov. is proposed. The type strain of Alkalispirochaeta cellulosivorans is JC227T ( = KCTC 15343T = NBRC 110105T). We also propose the reclassification of Spirochaeta sphaeroplastigenens, Spirochaeta odontotermitis, Spirochaeta alkalica and Spirochaeta americana as Alkalispirochaeta sphaeroplastigenens comb. nov. (type strain JC133T = KCTC 15220T = NBRC 109056T), Alkalispirochaeta odontotermitis comb. nov. (type strain JC202T = KCTC 15324T = NBRC 110104T), Alkalispirochaeta alkalica comb. nov. (type strain Z-7491T = DSM 8900T = ATCC 700262T) and Alkalispirochaeta americana comb. nov. (type strain ASpG1T = ATCC BAA-392T = DSM 14872T). The type species of Alkalispirochaeta gen. nov. is Alkalispirochaeta alkalica comb. nov.

  • Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic Spirochaete isolated from the termite Odontotermes obesus (Rambur) gut
    International Journal of Systematic and Evolutionary Microbiology, 2015
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    A Gram-stain-negative Spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( < 93.83 %) as the closest phylogenetic neighbours. However, mean DNA–DNA hydridization values between strain JC202T and S. sphaeroplastigenens JC133T, S. alkalica DSM 8900T ( = Z-7491T) and S. americana DSM 14872T ( = ASpG1T) were 55 ± 2, 22 ± 3 and 32 ± 1 %, respectively. On the basis of physiological, biochemical, chemotaxonomic (including metabolome) and genomic differences from the previously described taxa, strain JC202T is differentiated from other members of the genus Spirochaeta and is considered to represent a novel species, for which the name Spirochaeta odontotermitis sp. nov. is proposed. The type strain is JC202T ( = KCTC 15324T = NBRC 110104T).

  • Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic Spirochaete isolated from the termite Odontotermes obesus (Rambur) gut.
    International journal of systematic and evolutionary microbiology, 2015
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    A Gram-stain-negative Spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( 

  • Spirochaeta lutea sp. nov., isolated from marine habitats and emended description of the genus Spirochaeta.
    Systematic and Applied Microbiology, 2015
    Co-Authors: Y. Shivani, L. Tushar, Ch. Sasikala, Y Subhash, Ch. V. Ramana
    Abstract:

    Abstract Metagenome analysis of forty samples indicated a rich diversity of free living Spirochaetes among Indian habitats. Only 2–3% of the total bacterial diversity was represented by Spirochaetes with five distinct phylogenetic OTUs. A strain (JC230 T ) was brought to cultivation and its 16S rRNA gene sequence showed highest sequence similarity with that of Spirochaeta asiatica Z-7591 T (90.78%). Genome sequence analysis of strain JC230 T indicates the presence of 117 genes related to motility and chemotaxis. Strain JC230 T is an obligate anaerobe. It is helical shaped, Gram-stain-negative and catalase and oxidase negative. Strain JC230 T produces carotenoids and have all the genes related to carotenogenesis of spirilloxanthin series. Isoprenoid biosynthesis occurs through HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) pathway. Twelve bacterial haemoglobin and one flavohaemoglobin related genes are present in the genome of strain JC230 T . The genome of strain JC230 T showed the presence of heavy metal resistance related genes of cobalt, zinc, cadmium and arsenic. Six genes related to colicin V and bacteriocin production cluster are present. Major (>2%) fatty acids were C 12:0 , C 14:0 , iso -C 14:0 , iso -C 15:1 H/C 13:0 3OH, anteiso -C 15:0 , C 16:0 , and iso -C 16:0 . Strain JC230 T contains diphosphatylglycerol, phosphatydilglycerol, phosphatydilethanolamine, unidentified phospholipid (PL1) and unidentified lipids (L1-7). G + C mol% of strain JC230 T was 54.1%. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC230 T as a representative of a new species in the genus Spirochaeta , for which the name Spirochaeta lutea sp. nov. is proposed. The type strain is JC230 T (=KCTC 15387 T  = DSM 29074 T ).

L. Tushar - One of the best experts on this subject based on the ideXlab platform.

  • Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as n
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    An obligately anaerobic Spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( 

  • alkalispirochaeta cellulosivorans gen nov sp nov a cellulose hydrolysing alkaliphilic halotolerant bacterium isolated from the gut of a wood eating cockroach cryptocercus punctulatus and reclassification of four species of spirochaeta as new combinat
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    An obligately anaerobic Spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( < 92.7 %) as its closest phylogenetic neighbours. However, DNA–DNA hybridization between strain JC227T and S. sphaeroplastigenens JC133T, S. odontotermitis JC202T, S. alkalica DSM 8900T and S. americana DSM 14872T was 62 ± 2, 63, 58 ± 2 and 48 ± 4 %, respectively. Strain JC227T contained phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and six unidentified lipids. Summed feature C18 : 1ω7c/C18 : 1ω6c was the predominant cellular fatty acid, with significant proportions of C16 : 0, C14 : 0, C12 : 0, C15 : 1ω6c, C16 : 1ω5c, C16 : 1ω6c/C16 : 1ω7c and C17 : 0 2-OH. The DNA G+C content of strain JC227T was 55.5 mol%. On the basis of physiological, biochemical, chemotaxonomic (including metabolomic) and genomic differences from previously described taxa, strain JC227T can be differentiated from members of the genus Spirochaeta and represents a novel species of a new genus, for which the name Alkalispirochaeta cellulosivorans gen. nov., sp. nov. is proposed. The type strain of Alkalispirochaeta cellulosivorans is JC227T ( = KCTC 15343T = NBRC 110105T). We also propose the reclassification of Spirochaeta sphaeroplastigenens, Spirochaeta odontotermitis, Spirochaeta alkalica and Spirochaeta americana as Alkalispirochaeta sphaeroplastigenens comb. nov. (type strain JC133T = KCTC 15220T = NBRC 109056T), Alkalispirochaeta odontotermitis comb. nov. (type strain JC202T = KCTC 15324T = NBRC 110104T), Alkalispirochaeta alkalica comb. nov. (type strain Z-7491T = DSM 8900T = ATCC 700262T) and Alkalispirochaeta americana comb. nov. (type strain ASpG1T = ATCC BAA-392T = DSM 14872T). The type species of Alkalispirochaeta gen. nov. is Alkalispirochaeta alkalica comb. nov.

  • Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic Spirochaete isolated from the termite Odontotermes obesus (Rambur) gut
    International Journal of Systematic and Evolutionary Microbiology, 2015
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    A Gram-stain-negative Spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( < 93.83 %) as the closest phylogenetic neighbours. However, mean DNA–DNA hydridization values between strain JC202T and S. sphaeroplastigenens JC133T, S. alkalica DSM 8900T ( = Z-7491T) and S. americana DSM 14872T ( = ASpG1T) were 55 ± 2, 22 ± 3 and 32 ± 1 %, respectively. On the basis of physiological, biochemical, chemotaxonomic (including metabolome) and genomic differences from the previously described taxa, strain JC202T is differentiated from other members of the genus Spirochaeta and is considered to represent a novel species, for which the name Spirochaeta odontotermitis sp. nov. is proposed. The type strain is JC202T ( = KCTC 15324T = NBRC 110104T).

  • Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic Spirochaete isolated from the termite Odontotermes obesus (Rambur) gut.
    International journal of systematic and evolutionary microbiology, 2015
    Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. Ramana
    Abstract:

    A Gram-stain-negative Spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( 

  • Spirochaeta lutea sp. nov., isolated from marine habitats and emended description of the genus Spirochaeta.
    Systematic and Applied Microbiology, 2015
    Co-Authors: Y. Shivani, L. Tushar, Ch. Sasikala, Y Subhash, Ch. V. Ramana
    Abstract:

    Abstract Metagenome analysis of forty samples indicated a rich diversity of free living Spirochaetes among Indian habitats. Only 2–3% of the total bacterial diversity was represented by Spirochaetes with five distinct phylogenetic OTUs. A strain (JC230 T ) was brought to cultivation and its 16S rRNA gene sequence showed highest sequence similarity with that of Spirochaeta asiatica Z-7591 T (90.78%). Genome sequence analysis of strain JC230 T indicates the presence of 117 genes related to motility and chemotaxis. Strain JC230 T is an obligate anaerobe. It is helical shaped, Gram-stain-negative and catalase and oxidase negative. Strain JC230 T produces carotenoids and have all the genes related to carotenogenesis of spirilloxanthin series. Isoprenoid biosynthesis occurs through HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) pathway. Twelve bacterial haemoglobin and one flavohaemoglobin related genes are present in the genome of strain JC230 T . The genome of strain JC230 T showed the presence of heavy metal resistance related genes of cobalt, zinc, cadmium and arsenic. Six genes related to colicin V and bacteriocin production cluster are present. Major (>2%) fatty acids were C 12:0 , C 14:0 , iso -C 14:0 , iso -C 15:1 H/C 13:0 3OH, anteiso -C 15:0 , C 16:0 , and iso -C 16:0 . Strain JC230 T contains diphosphatylglycerol, phosphatydilglycerol, phosphatydilethanolamine, unidentified phospholipid (PL1) and unidentified lipids (L1-7). G + C mol% of strain JC230 T was 54.1%. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC230 T as a representative of a new species in the genus Spirochaeta , for which the name Spirochaeta lutea sp. nov. is proposed. The type strain is JC230 T (=KCTC 15387 T  = DSM 29074 T ).

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  • Spirochaeta dissipatitropha sp. nov., an alkaliphilic, obligately anaerobic bacterium, and emended description of the genus Spirochaeta Ehrenberg 1835
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009
    Co-Authors: Elena V. Pikuta, Richard B. Hoover, Asim K. Bej, Damien Marsic, William B. Whitman, Paul Krader
    Abstract:

    A novel obligately anaerobic, mesophilic, alkaliphilic Spirochaete, strain ASpC2T, was isolated from an anaerobic sediment of alkaline, hypersaline Owens Lake in California, USA. The Gram-negative cells are motile, helical in shape and 0.23×8.0–18.0 μm. Growth occurs within the following ranges: 13–41 °C, with optimal growth at 35 °C; 1–3 % (w/v) NaCl, with optimal growth at 2 % (w/v) NaCl; and pH 7.8–10.5, with optimal growth at pH 10.0. The novel isolate is strictly alkaliphilic and requires high concentrations of carbonate ions in the medium. It utilizes some sugars, some organic acids, some amino acids, Casamino acids, yeast extract and peptone. The main end products of glucose fermentation are CO2 and acetate. Strain ASpC2T is resistant to kanamycin and rifampicin, but sensitive to ampicillin, chloramphenicol, gentamicin and tetracycline. The DNA G+C content of the new isolate is 43.8 mol%, its genome size is 6×108 Da and the melting temperature of its genomic DNA is 71 °C. DNA–DNA hybridization experiments demonstrated 46 % similarity with the phylogenetically most closely related species, Spirochaeta asiatica Z-7591T. On the basis of physiological and molecular properties, the new isolate belongs taxonomically to a novel species within the genus Spirochaeta, for which the name Spirochaeta dissipatitropha sp. nov. is proposed (type strain, ASpC2T=ATCC BAA-1083T=JCM 12856T). S. dissipatitropha ASpC2T is the second strain in the genus (after Spirochaeta smaragdinae SEBR 4228T) that is able to use proteolysis products as the sole energy source, and additional tests have shown that other halo-alkaliphilic Spirochaetes (Spirochaeta americana, Spirochaeta alkalica and Spirochaeta africana) are also able to grow on yeast extract alone; therefore, an emended description for the genus Spirochaeta is given.

  • Spirochaeta dissipatitropha sp. nov., an alkaliphilic, obligately anaerobic bacterium, and emended description of the genus Spirochaeta Ehrenberg 1835.
    International journal of systematic and evolutionary microbiology, 2009
    Co-Authors: Elena V. Pikuta, Richard B. Hoover, Asim K. Bej, Damien Marsic, William B. Whitman, Paul Krader
    Abstract:

    A novel obligately anaerobic, mesophilic, alkaliphilic Spirochaete, strain ASpC2(T), was isolated from an anaerobic sediment of alkaline, hypersaline Owens Lake in California, USA. The Gram-negative cells are motile, helical in shape and 0.23 x 8.0-18.0 mum. Growth occurs within the following ranges: 13-41 degrees C, with optimal growth at 35 degrees C; 1-3 % (w/v) NaCl, with optimal growth at 2 % (w/v) NaCl; and pH 7.8-10.5, with optimal growth at pH 10.0. The novel isolate is strictly alkaliphilic and requires high concentrations of carbonate ions in the medium. It utilizes some sugars, some organic acids, some amino acids, Casamino acids, yeast extract and peptone. The main end products of glucose fermentation are CO(2) and acetate. Strain ASpC2(T) is resistant to kanamycin and rifampicin, but sensitive to ampicillin, chloramphenicol, gentamicin and tetracycline. The DNA G+C content of the new isolate is 43.8 mol%, its genome size is 6 x 10(8) Da and the melting temperature of its genomic DNA is 71 degrees C. DNA-DNA hybridization experiments demonstrated 46 % similarity with the phylogenetically most closely related species, Spirochaeta asiatica Z-7591(T). On the basis of physiological and molecular properties, the new isolate belongs taxonomically to a novel species within the genus Spirochaeta, for which the name Spirochaeta dissipatitropha sp. nov. is proposed (type strain, ASpC2(T)=ATCC BAA-1083(T)=JCM 12856(T)). S. dissipatitropha ASpC2(T) is the second strain in the genus (after Spirochaeta smaragdinae SEBR 4228(T)) that is able to use proteolysis products as the sole energy source, and additional tests have shown that other halo-alkaliphilic Spirochaetes (Spirochaeta americana, Spirochaeta alkalica and Spirochaeta africana) are also able to grow on yeast extract alone; therefore, an emended description for the genus Spirochaeta is given.

  • Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic Spirochaete isolated from soda Mono Lake in California.
    International Journal of Systematic and Evolutionary Microbiology, 2003
    Co-Authors: Richard B. Hoover, Elena V. Pikuta, Asim K. Bej, Damien Marsic, William B. Whitman, Jane Tang, Paul Krader
    Abstract:

    A novel, obligately anaerobic, mesophilic, haloalkaliphilic Spirochaete, strain ASpG1(T), was isolated from sediments of the alkaline, hypersaline Mono Lake in California, USA. Cells of the Gram-negative strain were motile and Spirochaete-shaped with sizes of 0.2-0.22 x 8-18 microm. Growth of the strain was observed between 10 and 44 degrees C (optimum 37 degrees C), in 2-12% (w/v) NaCl (optimum 3% NaCl) and between pH 8 and 10.5 (optimum pH 9.5). The novel strain was strictly alkaliphilic, required high concentrations of carbonates in the medium and was capable of utilizing D-glucose, fructose, maltose, sucrose, starch and D-mannitol. End products of glucose fermentation were H2, acetate, ethanol and formate. Strain ASpG(T) was resistant to kanamycin and rifampicin, but sensitive to gentamicin, tetracycline and chloramphenicol. The G + C content of its DNA was 58.5 mol%. DNA-DNA hybridization analysis of strain ASpG1(T) with its most closely related species, Spirochaeta alkalica Z-7491(T), revealed a hybridization value of only 48.7%. On the basis of its physiological and molecular properties, strain ASpG1(T) appears to represent a novel species of the genus Spirochaeta, for which the name Spirochaeta americana is proposed (type strain ASpG1(T) =ATCC BAA-392(T) = DSM 14872(T)).