The Experts below are selected from a list of 1830 Experts worldwide ranked by ideXlab platform
Susan Lucas - One of the best experts on this subject based on the ideXlab platform.
-
alkaline Lake Magadi in the East African Rift
2016Co-Authors: Konstantinos Liolos, Matt Nolan, Susan Lucas, Shweta Deshp, Carmen Scheuner, Jan Fang Cheng, Birte Abt, Hazuki Teshima, Brittany Held, Lynne A. GoodwinAbstract:Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692T) from th
-
Standards in Genomic Sciences (2013) 8:88-105 DOI:10.4056/sigs.3096473 The Genomic Standards Consortium
2016Co-Authors: Alla Lapidus, Matt Nolan, Susan Lucas, Nancy Hammon, Shweta Deshp, Roxanne Tapia, Lynne A. Goodwin, Sam Pitluck, Konstantinos Liolios, Natalia IvanovaAbstract:Genome sequence of the thermophilic fresh-water bacterium Spirochaeta caldaria type strain (H1T), reclassification of Spirochaeta caldaria, Spirochaeta stenostrepta, and Spirochaeta zuelzerae in the genus Treponema as Treponema caldaria comb. nov., Treponema stenostrepta comb. nov., and Treponema zuelzerae comb. nov., and emendation of the genus Treponem
-
Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692 T ) from the alkaline Lake Magadi in the East African Rift
Standards in Genomic Sciences, 2013Co-Authors: Konstantinos Liolos, Alla Lapidus, Matt Nolan, Susan Lucas, Carmen Scheuner, Shweta Deshpande, Birte Abt, Hazuki Teshima, Brittany Held, Jan Fang ChengAbstract:Spirochaeta africana Zhilina et al. 1996 is an anaerobic, aerotolerant, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain of the species, Z-7692T, was isolated in 1993 or earlier from a bacterial bloom in the brine under the trona layer in a shallow lagoon of the alkaline equatorial Lake Magadi in Kenya. Here we describe the features of this organism, together with the complete genome sequence, and annotation. Considering the pending reclassification of S. caldaria to the genus Treponema, S. africana is only the second ‘true’ member of the genus Spirochaeta with a genome-sequenced type strain to be published. The 3,285,855 bp long genome of strain Z-7692T with its 2,817 protein-coding and 57 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
-
genome sequence of the thermophilic fresh water bacterium Spirochaeta caldaria type strain h1 t reclassification of Spirochaeta caldaria Spirochaeta stenostrepta and Spirochaeta zuelzerae in the genus treponema as treponema caldaria comb nov treponem
Standards in Genomic Sciences, 2013Co-Authors: Birte Abt, Alla Lapidus, Matt Nolan, Carmen Scheuner, Markus Goker, Cliff Han, Monica Misra, Susan LucasAbstract:Spirochaeta caldaria Pohlschroeder et al. 1995 is an obligately anaerobic, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain, H1T, was isolated in 1990 from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA, and is of interest because it enhances the degradation of cellulose when grown in co-culture with Clostridium thermocellum. Here we provide a taxonomic re-evaluation for S. caldaria based on phylogenetic analyses of 16S rRNA sequences and whole genomes, and propose the reclassification of S. caldaria and two other Spirochaeta species as members of the emended genus Treponema. Whereas genera such as Borrelia and Sphaerochaeta possess well-distinguished genomic features related to their divergent lifestyles, the physiological and functional genomic characteristics of Spirochaeta and Treponema appear to be intermixed and are of little taxonomic value. The 3,239,340 bp long genome of strain H1T with its 2,869 protein-coding and 59 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
-
genome sequence of the thermophilic fresh water bacterium Spirochaeta caldaria type strain h1 t reclassification of Spirochaeta caldaria and Spirochaeta stenostrepta in the genus treponema as
Standards in Genomic Sciences, 2013Co-Authors: Birte Abt, Alla Lapidus, Matt Nolan, Susan Lucas, Nancy Hammon, Carmen Scheuner, Markus Goker, Cliff Han, Monica Misra, Shweta DeshpandeAbstract:Spirochaeta caldaria Pohlschroeder et al . 1995 is an obligately anaerobic, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain, H1 T , was isolated in 1990 from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA, and is of interest because it enhances the degradation of cellulose when grown in co-culture with Clostridium thermocellum . Here we provide a taxonomic re-evaluation for S. caldaria based on phylogenetic analyses of 16S rRNA sequences and whole genomes and propose the reclassification of it and two other Spirochaeta species as members of an emended genus Treponema. Whereas genera such as Borrelia and Sphaerochaeta possess well-distinguished genomic features related to their deviating lifestyle, not only physiology but also the functional genomic characteristics of Spirochaeta and Treponema appear to be intermixed and are of little taxonomic value. The 3,239,340 bp long genome of strain H1 T with its 2,869 protein-coding and 59 RNA genes is a part of the G enomic E ncyclopedia of B acteria and A rchaea project. doi:10.4056/sigs.3096473
Alla Lapidus - One of the best experts on this subject based on the ideXlab platform.
-
Standards in Genomic Sciences (2013) 8:88-105 DOI:10.4056/sigs.3096473 The Genomic Standards Consortium
2016Co-Authors: Alla Lapidus, Matt Nolan, Susan Lucas, Nancy Hammon, Shweta Deshp, Roxanne Tapia, Lynne A. Goodwin, Sam Pitluck, Konstantinos Liolios, Natalia IvanovaAbstract:Genome sequence of the thermophilic fresh-water bacterium Spirochaeta caldaria type strain (H1T), reclassification of Spirochaeta caldaria, Spirochaeta stenostrepta, and Spirochaeta zuelzerae in the genus Treponema as Treponema caldaria comb. nov., Treponema stenostrepta comb. nov., and Treponema zuelzerae comb. nov., and emendation of the genus Treponem
-
Standards in Genomic Sciences (2012) 6:194-209 DOI:10.4056/sigs.2796069 The Genomic Standards Consortium
2016Co-Authors: Birte Abt, Alla Lapidus, Matt Nolan, Nancy Hammon, Shweta Deshp, Carmen Scheuner, Jan Fang Cheng, Cliff Han, Roxanne TapiaAbstract:Complete genome sequence of the termite hindgut bacterium Spirochaeta coccoides type strain (SPN1T), reclassification in the genus Sphaerochaeta as Sphaerochaeta coccoides comb. nov. and emendations of the family Spirochaetaceae and the genu
-
Standards in Genomic Sciences (2010) 3:136-144 DOI:10.4056/sigs.1143106 The Genomic Standards Consortium Complete genome sequence of Spirochaeta smaragdinae type strain (SEBR 4228T)
2016Co-Authors: Konstantinos Mavromatis, Alla Lapidus, Matt Nolan, Jan Fang Cheng, Montri Yasawong, Olga Chertkov, Tijana Glavina, Del Rio, Hope Tice, Sam PitluckAbstract:Spirochaeta smaragdinae Magot et al. 1998 belongs to the family Spirochaetaceae. The spe-cies is Gram-negative, motile, obligately halophilic and strictly anaerobic and is of interest because it is able to ferment numerous polysaccharides. S. smaragdinae is the only species of the family Spirochaetaceae known to reduce thiosulfate or element sulfur to sulfide. This is the first complete genome sequence in the family Spirochaetaceae. The 4,653,970 bp long genome with its 4,363 protein-coding and 57 RNA genes is a part of the Genomic Encyclo-pedia of Bacteria and Archaea project. Introduction Strain SEBR 4228T ( = DSM 11293 = JCM 15392) is the type strain of the species Spirochaeta smarag
-
Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692 T ) from the alkaline Lake Magadi in the East African Rift
Standards in Genomic Sciences, 2013Co-Authors: Konstantinos Liolos, Alla Lapidus, Matt Nolan, Susan Lucas, Carmen Scheuner, Shweta Deshpande, Birte Abt, Hazuki Teshima, Brittany Held, Jan Fang ChengAbstract:Spirochaeta africana Zhilina et al. 1996 is an anaerobic, aerotolerant, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain of the species, Z-7692T, was isolated in 1993 or earlier from a bacterial bloom in the brine under the trona layer in a shallow lagoon of the alkaline equatorial Lake Magadi in Kenya. Here we describe the features of this organism, together with the complete genome sequence, and annotation. Considering the pending reclassification of S. caldaria to the genus Treponema, S. africana is only the second ‘true’ member of the genus Spirochaeta with a genome-sequenced type strain to be published. The 3,285,855 bp long genome of strain Z-7692T with its 2,817 protein-coding and 57 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
-
genome sequence of the thermophilic fresh water bacterium Spirochaeta caldaria type strain h1 t reclassification of Spirochaeta caldaria Spirochaeta stenostrepta and Spirochaeta zuelzerae in the genus treponema as treponema caldaria comb nov treponem
Standards in Genomic Sciences, 2013Co-Authors: Birte Abt, Alla Lapidus, Matt Nolan, Carmen Scheuner, Markus Goker, Cliff Han, Monica Misra, Susan LucasAbstract:Spirochaeta caldaria Pohlschroeder et al. 1995 is an obligately anaerobic, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain, H1T, was isolated in 1990 from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA, and is of interest because it enhances the degradation of cellulose when grown in co-culture with Clostridium thermocellum. Here we provide a taxonomic re-evaluation for S. caldaria based on phylogenetic analyses of 16S rRNA sequences and whole genomes, and propose the reclassification of S. caldaria and two other Spirochaeta species as members of the emended genus Treponema. Whereas genera such as Borrelia and Sphaerochaeta possess well-distinguished genomic features related to their divergent lifestyles, the physiological and functional genomic characteristics of Spirochaeta and Treponema appear to be intermixed and are of little taxonomic value. The 3,239,340 bp long genome of strain H1T with its 2,869 protein-coding and 59 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
Matt Nolan - One of the best experts on this subject based on the ideXlab platform.
-
alkaline Lake Magadi in the East African Rift
2016Co-Authors: Konstantinos Liolos, Matt Nolan, Susan Lucas, Shweta Deshp, Carmen Scheuner, Jan Fang Cheng, Birte Abt, Hazuki Teshima, Brittany Held, Lynne A. GoodwinAbstract:Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692T) from th
-
Standards in Genomic Sciences (2013) 8:88-105 DOI:10.4056/sigs.3096473 The Genomic Standards Consortium
2016Co-Authors: Alla Lapidus, Matt Nolan, Susan Lucas, Nancy Hammon, Shweta Deshp, Roxanne Tapia, Lynne A. Goodwin, Sam Pitluck, Konstantinos Liolios, Natalia IvanovaAbstract:Genome sequence of the thermophilic fresh-water bacterium Spirochaeta caldaria type strain (H1T), reclassification of Spirochaeta caldaria, Spirochaeta stenostrepta, and Spirochaeta zuelzerae in the genus Treponema as Treponema caldaria comb. nov., Treponema stenostrepta comb. nov., and Treponema zuelzerae comb. nov., and emendation of the genus Treponem
-
Standards in Genomic Sciences (2012) 6:194-209 DOI:10.4056/sigs.2796069 The Genomic Standards Consortium
2016Co-Authors: Birte Abt, Alla Lapidus, Matt Nolan, Nancy Hammon, Shweta Deshp, Carmen Scheuner, Jan Fang Cheng, Cliff Han, Roxanne TapiaAbstract:Complete genome sequence of the termite hindgut bacterium Spirochaeta coccoides type strain (SPN1T), reclassification in the genus Sphaerochaeta as Sphaerochaeta coccoides comb. nov. and emendations of the family Spirochaetaceae and the genu
-
Standards in Genomic Sciences (2010) 3:136-144 DOI:10.4056/sigs.1143106 The Genomic Standards Consortium Complete genome sequence of Spirochaeta smaragdinae type strain (SEBR 4228T)
2016Co-Authors: Konstantinos Mavromatis, Alla Lapidus, Matt Nolan, Jan Fang Cheng, Montri Yasawong, Olga Chertkov, Tijana Glavina, Del Rio, Hope Tice, Sam PitluckAbstract:Spirochaeta smaragdinae Magot et al. 1998 belongs to the family Spirochaetaceae. The spe-cies is Gram-negative, motile, obligately halophilic and strictly anaerobic and is of interest because it is able to ferment numerous polysaccharides. S. smaragdinae is the only species of the family Spirochaetaceae known to reduce thiosulfate or element sulfur to sulfide. This is the first complete genome sequence in the family Spirochaetaceae. The 4,653,970 bp long genome with its 4,363 protein-coding and 57 RNA genes is a part of the Genomic Encyclo-pedia of Bacteria and Archaea project. Introduction Strain SEBR 4228T ( = DSM 11293 = JCM 15392) is the type strain of the species Spirochaeta smarag
-
Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692 T ) from the alkaline Lake Magadi in the East African Rift
Standards in Genomic Sciences, 2013Co-Authors: Konstantinos Liolos, Alla Lapidus, Matt Nolan, Susan Lucas, Carmen Scheuner, Shweta Deshpande, Birte Abt, Hazuki Teshima, Brittany Held, Jan Fang ChengAbstract:Spirochaeta africana Zhilina et al. 1996 is an anaerobic, aerotolerant, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain of the species, Z-7692T, was isolated in 1993 or earlier from a bacterial bloom in the brine under the trona layer in a shallow lagoon of the alkaline equatorial Lake Magadi in Kenya. Here we describe the features of this organism, together with the complete genome sequence, and annotation. Considering the pending reclassification of S. caldaria to the genus Treponema, S. africana is only the second ‘true’ member of the genus Spirochaeta with a genome-sequenced type strain to be published. The 3,285,855 bp long genome of strain Z-7692T with its 2,817 protein-coding and 57 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
Carmen Scheuner - One of the best experts on this subject based on the ideXlab platform.
-
alkaline Lake Magadi in the East African Rift
2016Co-Authors: Konstantinos Liolos, Matt Nolan, Susan Lucas, Shweta Deshp, Carmen Scheuner, Jan Fang Cheng, Birte Abt, Hazuki Teshima, Brittany Held, Lynne A. GoodwinAbstract:Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692T) from th
-
Standards in Genomic Sciences (2012) 6:194-209 DOI:10.4056/sigs.2796069 The Genomic Standards Consortium
2016Co-Authors: Birte Abt, Alla Lapidus, Matt Nolan, Nancy Hammon, Shweta Deshp, Carmen Scheuner, Jan Fang Cheng, Cliff Han, Roxanne TapiaAbstract:Complete genome sequence of the termite hindgut bacterium Spirochaeta coccoides type strain (SPN1T), reclassification in the genus Sphaerochaeta as Sphaerochaeta coccoides comb. nov. and emendations of the family Spirochaetaceae and the genu
-
Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692 T ) from the alkaline Lake Magadi in the East African Rift
Standards in Genomic Sciences, 2013Co-Authors: Konstantinos Liolos, Alla Lapidus, Matt Nolan, Susan Lucas, Carmen Scheuner, Shweta Deshpande, Birte Abt, Hazuki Teshima, Brittany Held, Jan Fang ChengAbstract:Spirochaeta africana Zhilina et al. 1996 is an anaerobic, aerotolerant, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain of the species, Z-7692T, was isolated in 1993 or earlier from a bacterial bloom in the brine under the trona layer in a shallow lagoon of the alkaline equatorial Lake Magadi in Kenya. Here we describe the features of this organism, together with the complete genome sequence, and annotation. Considering the pending reclassification of S. caldaria to the genus Treponema, S. africana is only the second ‘true’ member of the genus Spirochaeta with a genome-sequenced type strain to be published. The 3,285,855 bp long genome of strain Z-7692T with its 2,817 protein-coding and 57 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
-
genome sequence of the thermophilic fresh water bacterium Spirochaeta caldaria type strain h1 t reclassification of Spirochaeta caldaria Spirochaeta stenostrepta and Spirochaeta zuelzerae in the genus treponema as treponema caldaria comb nov treponem
Standards in Genomic Sciences, 2013Co-Authors: Birte Abt, Alla Lapidus, Matt Nolan, Carmen Scheuner, Markus Goker, Cliff Han, Monica Misra, Susan LucasAbstract:Spirochaeta caldaria Pohlschroeder et al. 1995 is an obligately anaerobic, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain, H1T, was isolated in 1990 from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA, and is of interest because it enhances the degradation of cellulose when grown in co-culture with Clostridium thermocellum. Here we provide a taxonomic re-evaluation for S. caldaria based on phylogenetic analyses of 16S rRNA sequences and whole genomes, and propose the reclassification of S. caldaria and two other Spirochaeta species as members of the emended genus Treponema. Whereas genera such as Borrelia and Sphaerochaeta possess well-distinguished genomic features related to their divergent lifestyles, the physiological and functional genomic characteristics of Spirochaeta and Treponema appear to be intermixed and are of little taxonomic value. The 3,239,340 bp long genome of strain H1T with its 2,869 protein-coding and 59 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
-
genome sequence of the thermophilic fresh water bacterium Spirochaeta caldaria type strain h1 t reclassification of Spirochaeta caldaria and Spirochaeta stenostrepta in the genus treponema as
Standards in Genomic Sciences, 2013Co-Authors: Birte Abt, Alla Lapidus, Matt Nolan, Susan Lucas, Nancy Hammon, Carmen Scheuner, Markus Goker, Cliff Han, Monica Misra, Shweta DeshpandeAbstract:Spirochaeta caldaria Pohlschroeder et al . 1995 is an obligately anaerobic, spiral-shaped bacterium that is motile via periplasmic flagella. The type strain, H1 T , was isolated in 1990 from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA, and is of interest because it enhances the degradation of cellulose when grown in co-culture with Clostridium thermocellum . Here we provide a taxonomic re-evaluation for S. caldaria based on phylogenetic analyses of 16S rRNA sequences and whole genomes and propose the reclassification of it and two other Spirochaeta species as members of an emended genus Treponema. Whereas genera such as Borrelia and Sphaerochaeta possess well-distinguished genomic features related to their deviating lifestyle, not only physiology but also the functional genomic characteristics of Spirochaeta and Treponema appear to be intermixed and are of little taxonomic value. The 3,239,340 bp long genome of strain H1 T with its 2,869 protein-coding and 59 RNA genes is a part of the G enomic E ncyclopedia of B acteria and A rchaea project. doi:10.4056/sigs.3096473
Ch. V. Ramana - One of the best experts on this subject based on the ideXlab platform.
-
Description of 'Candidatus MariSpirochaeta associata' and reclassification of Spirochaeta bajacaliforniensis, Spirochaeta smaragdinae and Spirochaeta sinaica to a new genus SediminiSpirochaeta gen. nov. as SediminiSpirochaeta bajacaliforniensis comb.
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Y. Shivani, Ch. Sasikala, Y Subhash, Ch. V. RamanaAbstract:Strain JC231 was isolated from a coastal saline habitat of Gujarat and was identified based on 16S rRNA gene sequence analysis as a member belonging to the genus Spirochaeta and showed highest sequence similarity ( 5 %) fatty acids. Strain JC231 contains diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and six unidentified lipids (L1-L6). G+C content of strain JC231 was 55.7 mol%. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC231 as a representative of a new genus and species in the family Spirochaetaceae, for which the name 'CandidatusMariSpirochaeta associata' is proposed. Strain JC231 is deposited as a defined co-culture with Desulfovibrio sp. JC271 to DSMZ (DSM 29857) and KCTC (KCTC 15472). Based on phenotypic, genotypic and phylogenetic analyses, we also propose the reclassification of Spirochaeta bajacaliforniensis as SediminiSpirochaeta bajacaliforniensis gen. nov., comb. nov., Spirochaeta smaragdinae as SediminiSpirochaeta smaragdinae comb. nov. and Spirochaeta sinaica as SediminiSpirochaeta sinaica comb. nov.
-
AlkaliSpirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as n
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. RamanaAbstract:An obligately anaerobic spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta (
-
alkaliSpirochaeta cellulosivorans gen nov sp nov a cellulose hydrolysing alkaliphilic halotolerant bacterium isolated from the gut of a wood eating cockroach cryptocercus punctulatus and reclassification of four species of Spirochaeta as new combinat
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. RamanaAbstract:An obligately anaerobic spirochaete designated strain JC227T was isolated from the gut of a wood-eating cockroach, Cryptocercus punctulatus (Scudder), from the Rann of Kutch, Gujarat, India. Strain JC227T was Gram-stain-negative, mesophilic, halotolerant and alkaliphilic. Based on 16S rRNA gene sequence analysis, strain JC227T belongs to the genus Spirochaeta, with Spirochaeta sphaeroplastigenens JC133T (99.51 %), S. odontotermitis JC202T (99.30 %), S. alkalica Z-7491T (99.10 %), S. americana (98.54 %) and other members of the genus Spirochaeta ( < 92.7 %) as its closest phylogenetic neighbours. However, DNA–DNA hybridization between strain JC227T and S. sphaeroplastigenens JC133T, S. odontotermitis JC202T, S. alkalica DSM 8900T and S. americana DSM 14872T was 62 ± 2, 63, 58 ± 2 and 48 ± 4 %, respectively. Strain JC227T contained phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and six unidentified lipids. Summed feature C18 : 1ω7c/C18 : 1ω6c was the predominant cellular fatty acid, with significant proportions of C16 : 0, C14 : 0, C12 : 0, C15 : 1ω6c, C16 : 1ω5c, C16 : 1ω6c/C16 : 1ω7c and C17 : 0 2-OH. The DNA G+C content of strain JC227T was 55.5 mol%. On the basis of physiological, biochemical, chemotaxonomic (including metabolomic) and genomic differences from previously described taxa, strain JC227T can be differentiated from members of the genus Spirochaeta and represents a novel species of a new genus, for which the name AlkaliSpirochaeta cellulosivorans gen. nov., sp. nov. is proposed. The type strain of AlkaliSpirochaeta cellulosivorans is JC227T ( = KCTC 15343T = NBRC 110105T). We also propose the reclassification of Spirochaeta sphaeroplastigenens, Spirochaeta odontotermitis, Spirochaeta alkalica and Spirochaeta americana as AlkaliSpirochaeta sphaeroplastigenens comb. nov. (type strain JC133T = KCTC 15220T = NBRC 109056T), AlkaliSpirochaeta odontotermitis comb. nov. (type strain JC202T = KCTC 15324T = NBRC 110104T), AlkaliSpirochaeta alkalica comb. nov. (type strain Z-7491T = DSM 8900T = ATCC 700262T) and AlkaliSpirochaeta americana comb. nov. (type strain ASpG1T = ATCC BAA-392T = DSM 14872T). The type species of AlkaliSpirochaeta gen. nov. is AlkaliSpirochaeta alkalica comb. nov.
-
Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic spirochaete isolated from the termite Odontotermes obesus (Rambur) gut
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. RamanaAbstract:A Gram-stain-negative spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta ( < 93.83 %) as the closest phylogenetic neighbours. However, mean DNA–DNA hydridization values between strain JC202T and S. sphaeroplastigenens JC133T, S. alkalica DSM 8900T ( = Z-7491T) and S. americana DSM 14872T ( = ASpG1T) were 55 ± 2, 22 ± 3 and 32 ± 1 %, respectively. On the basis of physiological, biochemical, chemotaxonomic (including metabolome) and genomic differences from the previously described taxa, strain JC202T is differentiated from other members of the genus Spirochaeta and is considered to represent a novel species, for which the name Spirochaeta odontotermitis sp. nov. is proposed. The type strain is JC202T ( = KCTC 15324T = NBRC 110104T).
-
Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic spirochaete isolated from the termite Odontotermes obesus (Rambur) gut.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: T. Sravanthi, L. Tushar, Ch. Sasikala, Ch. V. RamanaAbstract:A Gram-stain-negative spirochaete (strain JC202T) was isolated from the gut of the termite Odontotermes obesus (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202T was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202T possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. C18 : 1ω7c was the predominant cellular fatty acid with significant proportions of C16 : 0, C18 : 1ω9c, C14 : 0, C18 : 0, C16 : 1ω5c, C18 : 1ω5c and C20 : 1ω9c. The DNA G+C content of strain JC202T was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202T is considered to belong to the genus Spirochaeta with Spirochaeta sphaeroplastigenens JC133T (100 % similarity), Spirochaeta alkalica Z-7491T (99.92 %), Spirochaeta americana ATCC BAA-392T (99.47 %) and other members of the genus Spirochaeta (