The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Eunice Valduga - One of the best experts on this subject based on the ideXlab platform.
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Use of low-cost agro products as substrate in semi-continuous process to obtain carotenoids by Sporidiobolus salmonicolor
Biocatalysis and Agricultural Biotechnology, 2017Co-Authors: Rosicler Colet, Marco Luccio, Letícia Urnau, Jaine Bampi, Jamile Zeni, Bruna Bernar Dias, Eliseu Rodrigues, Rosângela Assis Jacques, Eunice ValdugaAbstract:Abstract This work has studied the semi-continuous carotenoids production by Sporidiobolus salmonicolor (CBS 2636) comparing the use of complex substrates and agroindustrial residues. The maximum total carotenoids concentration was 4376 μg L−1 in a 264 h period with complex substrates (80 g L−1 glycerol, 5 g L−1 malt extract, and 15 g L−1 peptone) and 7388 μg L−1 in a 288 h period with agroindustrial residues (80 g L−1 crude glycerol, 80 g L−1 corn maceration water, and 20 g L−1 rice parboiling water), showing an of approximate 54% increase, under the following conditions: 25 °C, pHinitial of 4.0, 180 rpm agitation rate, 1.5 vvm aeration rate, with 50% working volume cut. The maximum productivity on total carotenoids was 34.8 and 41.4 g L−1 h−1, the maximum productivity on cells was 0.058 and 0.104 g L−1 h−1, with specific maximum growth speeds of 0.05 and 0.08 h−1, obtaining the major carotenoid all-trans-β-carotene (83% and 81%), respectively.
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237 PUBLICATIONS 2,114 CITATIONS SEE PROFILE
2016Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Marco Luccio, Agenor Furigo JúniorAbstract:Study of the bio‐production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using pre‐treated agro‐industrial substrate
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Fed-batch production of carotenoids by Sporidiobolus salmonicolor (CBS 2636): kinetic and stoichiometric parameters
European Food Research and Technology, 2015Co-Authors: Rosicler Colet, Marco Luccio, Eunice ValdugaAbstract:This work studied the fed-batch production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using agro-industrial waste as substrates (glycerol, corn steep liquor, parboiled rice waste). The feed rate was varied after 24 h of bioproduction, using glycerol (85 % pure and crude), peptone and malt extract in the feed. The maximum concentration of total carotenoids found was 4,400 μg/L in 96 h of process at the conditions: 25 °C, initial pH 4.0, stirring rate 180 rpm, aeration rate of 1.5 vvm (air volume per volume of culture medium per minute), 80 g/L glycerol, 15 g/L peptone and 5 g/L malt extract, with a feed rate of 112.5 mL each 12 h. The use of crude glycerol yielded maximum carotenoids of 3,009 µg/L. The specific production of carotenoids ( Y _ p/x ) was 859.1 μg/g, and maximum specific growth rate ( μ _max) was 0.05 h^−1. The highest cell production in this study was 0.085 g/L h.
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Use of chemical, enzymatic and ultrasound-assisted methods for cell disruption to obtain carotenoids
Biocatalysis and Agricultural Biotechnology, 2013Co-Authors: L.m. Monks, Aline A. Rigo, Marcio A. Mazutti, J. Vladimir Oliveira, Eunice ValdugaAbstract:Abstract This work is focused on the cell disruption of Sporidiobolus salmonicolor (CBS 2636) for releasing carotenoids by means of enzymatic, chemical and ultrasound-assisted treatments. Maximum amount of carotenoids recovered was 572.70 µg L −1 obtained by enzymatic hydrolysis using enzyme concentration of 3.4 g of enzyme per gram of dry cell at 35 °C. Using chemical and ultrasound-assisted methods for cell disruption only 26% of the amount recovered using enzymatic hydrolysis for cell disruption was obtained. Ultrasound-assisted cell disruption alone or combined presented lower performance. The results obtained here pointed out that application of enzymes for cell disruption is an important contributor to produce carotenoids from microbial cells, using green chemistry principles to guide new products/process development.
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Assessment of Carotenoids Recovery through Cell Rupture of Sporidiobolus salmonicolor CBS 2636 Using Compressed Fluids
Food and Bioprocess Technology, 2012Co-Authors: Luciana Monks, Lídia Tiggamann, Marcio A. Mazuti, Vladimir J. Oliveira, Eunice ValdugaAbstract:The increasing demand for carotenoids by industries has drawn attention to their bio-production. Since pigments are intracellular, extraction steps are then needed after cell cultivation. In this work, different strategies for extraction of carotenoid pigments from Sporidiobolus salmonicolor (CBS 2636) were investigated. The cell rupture was carried out using dimethyl sulfoxide (DMSO), two pure compressed fluids, supercritical carbon dioxide and propane, and also a combination of pressurized fluid treatment followed by liquid DMSO. Dichloromethane, ethanol, ethyl acetate, and acetone were tested for the carotenoids extraction. Results obtained show that when multiple solvents were used a synergetic effect on the extent of carotenoids recovery was verified. Maximum concentration of total carotenoids (2,875 μg/L) was obtained in the treatment using supercritical CO_2 (300 bar/120 min) followed by dimethyl sulfoxide to disrupt the cell, and then the extraction with a solution of acetone/methanol (7:3, v / v ).
Marco Luccio - One of the best experts on this subject based on the ideXlab platform.
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Use of low-cost agro products as substrate in semi-continuous process to obtain carotenoids by Sporidiobolus salmonicolor
Biocatalysis and Agricultural Biotechnology, 2017Co-Authors: Rosicler Colet, Marco Luccio, Letícia Urnau, Jaine Bampi, Jamile Zeni, Bruna Bernar Dias, Eliseu Rodrigues, Rosângela Assis Jacques, Eunice ValdugaAbstract:Abstract This work has studied the semi-continuous carotenoids production by Sporidiobolus salmonicolor (CBS 2636) comparing the use of complex substrates and agroindustrial residues. The maximum total carotenoids concentration was 4376 μg L−1 in a 264 h period with complex substrates (80 g L−1 glycerol, 5 g L−1 malt extract, and 15 g L−1 peptone) and 7388 μg L−1 in a 288 h period with agroindustrial residues (80 g L−1 crude glycerol, 80 g L−1 corn maceration water, and 20 g L−1 rice parboiling water), showing an of approximate 54% increase, under the following conditions: 25 °C, pHinitial of 4.0, 180 rpm agitation rate, 1.5 vvm aeration rate, with 50% working volume cut. The maximum productivity on total carotenoids was 34.8 and 41.4 g L−1 h−1, the maximum productivity on cells was 0.058 and 0.104 g L−1 h−1, with specific maximum growth speeds of 0.05 and 0.08 h−1, obtaining the major carotenoid all-trans-β-carotene (83% and 81%), respectively.
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237 PUBLICATIONS 2,114 CITATIONS SEE PROFILE
2016Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Marco Luccio, Agenor Furigo JúniorAbstract:Study of the bio‐production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using pre‐treated agro‐industrial substrate
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Fed-batch production of carotenoids by Sporidiobolus salmonicolor (CBS 2636): kinetic and stoichiometric parameters
European Food Research and Technology, 2015Co-Authors: Rosicler Colet, Marco Luccio, Eunice ValdugaAbstract:This work studied the fed-batch production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using agro-industrial waste as substrates (glycerol, corn steep liquor, parboiled rice waste). The feed rate was varied after 24 h of bioproduction, using glycerol (85 % pure and crude), peptone and malt extract in the feed. The maximum concentration of total carotenoids found was 4,400 μg/L in 96 h of process at the conditions: 25 °C, initial pH 4.0, stirring rate 180 rpm, aeration rate of 1.5 vvm (air volume per volume of culture medium per minute), 80 g/L glycerol, 15 g/L peptone and 5 g/L malt extract, with a feed rate of 112.5 mL each 12 h. The use of crude glycerol yielded maximum carotenoids of 3,009 µg/L. The specific production of carotenoids ( Y _ p/x ) was 859.1 μg/g, and maximum specific growth rate ( μ _max) was 0.05 h^−1. The highest cell production in this study was 0.085 g/L h.
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Evaluation of aeration and substrate concentration on the production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) in bioreactor
European Food Research and Technology, 2011Co-Authors: Eunice Valduga, Pihetra Oliveira Tatsch, Marco Luccio, Lídia Tiggemann, Cátia Regina Martin Schwartz, Helen TreichelAbstract:This study aimed at optimising the cultivation conditions for the production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) in a bioreactor. The maximum content of total carotenoids in the full factorial design 2² was 3131.3 μg/L in synthetic medium with 80 g/L of glucose, 15 g/L of peptone, 5 g/L of malt extract, aeration of 1.5 vvm, agitation of 180 rpm, initial pH of 4.0 at 25 °C. In the kinetic study, we could observe that the bioproduction of carotenoids is associated with cell growth in the exponential phase, and the average specific growth (μ) in bioreactor is 0.046 h^−1 with a maximum yield of 0.19 g cells/L h. The maximum yield of carotenoids (60.0 μg/Lh) is observed at 50-h bioproduction. The conversion factor for total organic carbon (TOC) in cells (Y_X/_SCOT) was 2.97 g/g (0–50 h) and 0.254 g/g (50–100 h), the conversion factor glucose into cells (Y_X/Sglicose) was 0.168 g/g (0–100 h). The specific production of carotenoids (Y_P/_X) was 390 μg of carotenoids per gram of cells, the conversion factor of carbon in the product (Y_P/SCOT) was 107.8 μg/g (0–50 h) and 34.4 μg/g (50–100 h), whereas the factor Y_P/_Sglicose was 69.59 μg/g. The agitation and aeration provided better homogeneity in the culture medium, and hence greater availability of nutrients and oxygen, leading to higher production of carotenoids.
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Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) Microextração em Fase Sólida (MEFS) de compostos voláteis produzidos por Sporidiobolus salmonicolor (CBS 2636)
2010Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Irajá Do Nascimento Filho, Agenor Furigo Junior, Marco LuccioAbstract:The aim of the present study was the assessment of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) using methyl and ethyl ricinoleate, ricinoleic acid and castor oil as precursors. The analysis of the volatile organic compounds was carried out using Head Space Solid Phase Micro-Extraction (HS - SPME). Factorial experimental design was used for investigating extraction conditions, verifying stirring rate (0-400 rpm), temperature (25-60 °C), extraction time (10-30 minutes), and sample volume (2-3 mL). The identification of volatile organic compounds was carried out by Gas Chromatography with Mass Spectrum Detector (GC/MSD). The conditions that resulted in maximum extraction were: 60 °C, 10 minutes extraction, no stirring, sample volume of 2.0 mL, and addition of saturated KCl (1:10 v/v). In the bio-production of volatile organic compounds the effect of stirring rate (120-200 rpm), temperature (23-33 °C), pH (4.08.0), precursor concentration (0.02-0.1%), mannitol (0-6%), and asparagine concentration (0-0.2%) was investigated. The bio-production at 28 °C, 160 rpm, pH 6,0 and with the addition of 0.02% ricinoleic acid to the medium yielded the highest production of VOCs, identified as 1,4-butanediol, 1,2,2-trimethylciclopropilamine, beta-ionone; 2,3-butanodione, pentanal, tetradecane, 2-isononenal, 4-octen-3-one, propanoic acid, and octadecane.
Alexsandra Valério - One of the best experts on this subject based on the ideXlab platform.
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237 PUBLICATIONS 2,114 CITATIONS SEE PROFILE
2016Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Marco Luccio, Agenor Furigo JúniorAbstract:Study of the bio‐production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using pre‐treated agro‐industrial substrate
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Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) Microextração em Fase Sólida (MEFS) de compostos voláteis produzidos por Sporidiobolus salmonicolor (CBS 2636)
2010Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Irajá Do Nascimento Filho, Agenor Furigo Junior, Marco LuccioAbstract:The aim of the present study was the assessment of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) using methyl and ethyl ricinoleate, ricinoleic acid and castor oil as precursors. The analysis of the volatile organic compounds was carried out using Head Space Solid Phase Micro-Extraction (HS - SPME). Factorial experimental design was used for investigating extraction conditions, verifying stirring rate (0-400 rpm), temperature (25-60 °C), extraction time (10-30 minutes), and sample volume (2-3 mL). The identification of volatile organic compounds was carried out by Gas Chromatography with Mass Spectrum Detector (GC/MSD). The conditions that resulted in maximum extraction were: 60 °C, 10 minutes extraction, no stirring, sample volume of 2.0 mL, and addition of saturated KCl (1:10 v/v). In the bio-production of volatile organic compounds the effect of stirring rate (120-200 rpm), temperature (23-33 °C), pH (4.08.0), precursor concentration (0.02-0.1%), mannitol (0-6%), and asparagine concentration (0-0.2%) was investigated. The bio-production at 28 °C, 160 rpm, pH 6,0 and with the addition of 0.02% ricinoleic acid to the medium yielded the highest production of VOCs, identified as 1,4-butanediol, 1,2,2-trimethylciclopropilamine, beta-ionone; 2,3-butanodione, pentanal, tetradecane, 2-isononenal, 4-octen-3-one, propanoic acid, and octadecane.
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Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636)
Ciência e Tecnologia de Alimentos, 2010Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Irajá Do Nascimento Filho, Agenor Furigo Junior, Marco LuccioAbstract:O objetivo do presente estudo foi avaliar os compostos orgânicos volateis produzidos por Sporidiobolus salmonicolor (CBS 2636) utilizando metil e etil ricinoleato, acido ricinoleico e oleo de mamona como precursores. A analise dos compostos volateis produzidos foi conduzida por Micro-Extracao em Fase Solida (MEFS). A tecnica de planejamento experimental foi utilizada na avaliacao das condicoes de extracao, na qual se avaliaram a agitacao (0-400 rpm), a temperatura (25-60 oC), o tempo de extracao (10-30 minutos) e o volume da amostra (2-3 mL). A identificacao dos compostos foi realizada por Cromatografia a Gas com deteccao por Espectrometria de Massas (CG/EM). As condicoes que resultaram na maxima extracao foram: 60 oC, 10 minutos de extracao, sem agitacao, volume de amostra de 2,0 mL e adicao de solucao de KCl saturada (1:10 v/v). Na bioproducao dos compostos volateis, o efeito da agitacao (120-200 rpm), da temperatura (23-33 oC), do pH (4,0-8,0), da concentracao do precursor (0,02 a 0,1%), da concentracao de manitol (0 a 6%) e de asparagina (0 a 0,2%) foi avaliado. Na condicao de 28 oC, 160 rpm, pH 6,0 e com a adicao de 0,02% de acido ricinoleico ao meio, foi atingida a maxima producao dos compostos volateis, identificados como: 1,4-butanodiol, 1,2,2-trimetilciclopropilamina, beta-ionona; 2,3-butanodiona, pentanal, tetradecano, 2-isononenal, 4-octen-3-ona, acido propanoico e octadecano.
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Assessment of Cell Disruption and Carotenoids Extraction from Sporidiobolus salmonicolor (CBS 2636)
Food and Bioprocess Technology, 2009Co-Authors: Eunice Valduga, Alexsandra Valério, Pihetra Oliveira Tatsch, Helen Treichel, Agenor Furigo, Marco LuccioAbstract:The increasing demand for carotenoids by industries has drawn attention to their bio-production. Since pigments are intracellular, extraction steps are then needed after cell cultivation. In this work, different strategies for extraction of carotenoid pigments from Sporidiobolus salmonicolor (CBS 2636) were studied. Different solvents (dimethyl sulfoxide, petroleum ether, hexane, ethyl acetate, chloroform, and acetone), liquid N_2, and diatomaceous earth were used to disrupt the cell and thus release the intracellular carotenoids. The results of this study showed that when multiple solvents were used, a synergistic effect on the extent of carotenoids recovery was obtained. Maximum concentration of total carotenoids (913 μg/L) was obtained in the treatment using liquid N_2 and dimethyl sulfoxide to disrupt the cell, followed by the extraction with a solution of acetone/methanol (7:3, v / v ).
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Kinetic and Stoichiometric Parameters in the Production of Carotenoids by Sporidiobolus salmonicolor (CBS 2636) in Synthetic and Agroindustrial Media
Applied Biochemistry and Biotechnology, 2009Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Agenor Furigo Júnior, Marco LuccioAbstract:With the objective of determining the kinetic behavior (growth, substrate, pH, and carotenoid production) and obtain the stoichiometric parameters of the fermentative process by Sporidiobolus salmonicolor in synthetic and agroindustrial media, fermentations were carried out in shaken flasks at 25°C, 180 rpm, and initial pH of 4.0 for 120 h in the dark, sampling every 6 h. The maximum concentrations of total carotenoids in synthetic (913 μg/L) and agroindustrial (502 μg/L) media were attained approximately 100 h after the start of the fermentative process. Carotenoid bioproduction is associated with cell growth and the ratio between carotenoid production and cell growth ( Y _P/X) is 176 and 163 μg/g in the synthetic and agroindustrial media, respectively. The pH of the agroindustrial fermentation medium varied from 4.2 to 8.5 during the fermentation. The specific growth rate ( μ _X) for S . salmonicolor in synthetic and agroindustrial media was 0.07 and 0.04 h^−1, respectively. The synthetic medium allowed for greater productivity, obtaining maximum cell productivity ( P _x) of 0.08 g L^−1 h^−1 and maximum total carotenoid productivity ( P _car) of 14.2 μg L^−1 h^−1. Knowledge of the kinetics of a fermentative process is of extreme importance when transposing a laboratory experiment to an industrial scale, as well as making a quantitative comparison between different culture conditions.
Helen Treichel - One of the best experts on this subject based on the ideXlab platform.
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237 PUBLICATIONS 2,114 CITATIONS SEE PROFILE
2016Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Marco Luccio, Agenor Furigo JúniorAbstract:Study of the bio‐production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using pre‐treated agro‐industrial substrate
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Evaluation of aeration and substrate concentration on the production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) in bioreactor
European Food Research and Technology, 2011Co-Authors: Eunice Valduga, Pihetra Oliveira Tatsch, Marco Luccio, Lídia Tiggemann, Cátia Regina Martin Schwartz, Helen TreichelAbstract:This study aimed at optimising the cultivation conditions for the production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) in a bioreactor. The maximum content of total carotenoids in the full factorial design 2² was 3131.3 μg/L in synthetic medium with 80 g/L of glucose, 15 g/L of peptone, 5 g/L of malt extract, aeration of 1.5 vvm, agitation of 180 rpm, initial pH of 4.0 at 25 °C. In the kinetic study, we could observe that the bioproduction of carotenoids is associated with cell growth in the exponential phase, and the average specific growth (μ) in bioreactor is 0.046 h^−1 with a maximum yield of 0.19 g cells/L h. The maximum yield of carotenoids (60.0 μg/Lh) is observed at 50-h bioproduction. The conversion factor for total organic carbon (TOC) in cells (Y_X/_SCOT) was 2.97 g/g (0–50 h) and 0.254 g/g (50–100 h), the conversion factor glucose into cells (Y_X/Sglicose) was 0.168 g/g (0–100 h). The specific production of carotenoids (Y_P/_X) was 390 μg of carotenoids per gram of cells, the conversion factor of carbon in the product (Y_P/SCOT) was 107.8 μg/g (0–50 h) and 34.4 μg/g (50–100 h), whereas the factor Y_P/_Sglicose was 69.59 μg/g. The agitation and aeration provided better homogeneity in the culture medium, and hence greater availability of nutrients and oxygen, leading to higher production of carotenoids.
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Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636)
Ciência e Tecnologia de Alimentos, 2010Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Irajá Do Nascimento Filho, Agenor Furigo Junior, Marco LuccioAbstract:O objetivo do presente estudo foi avaliar os compostos orgânicos volateis produzidos por Sporidiobolus salmonicolor (CBS 2636) utilizando metil e etil ricinoleato, acido ricinoleico e oleo de mamona como precursores. A analise dos compostos volateis produzidos foi conduzida por Micro-Extracao em Fase Solida (MEFS). A tecnica de planejamento experimental foi utilizada na avaliacao das condicoes de extracao, na qual se avaliaram a agitacao (0-400 rpm), a temperatura (25-60 oC), o tempo de extracao (10-30 minutos) e o volume da amostra (2-3 mL). A identificacao dos compostos foi realizada por Cromatografia a Gas com deteccao por Espectrometria de Massas (CG/EM). As condicoes que resultaram na maxima extracao foram: 60 oC, 10 minutos de extracao, sem agitacao, volume de amostra de 2,0 mL e adicao de solucao de KCl saturada (1:10 v/v). Na bioproducao dos compostos volateis, o efeito da agitacao (120-200 rpm), da temperatura (23-33 oC), do pH (4,0-8,0), da concentracao do precursor (0,02 a 0,1%), da concentracao de manitol (0 a 6%) e de asparagina (0 a 0,2%) foi avaliado. Na condicao de 28 oC, 160 rpm, pH 6,0 e com a adicao de 0,02% de acido ricinoleico ao meio, foi atingida a maxima producao dos compostos volateis, identificados como: 1,4-butanodiol, 1,2,2-trimetilciclopropilamina, beta-ionona; 2,3-butanodiona, pentanal, tetradecano, 2-isononenal, 4-octen-3-ona, acido propanoico e octadecano.
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Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) Microextração em Fase Sólida (MEFS) de compostos voláteis produzidos por Sporidiobolus salmonicolor (CBS 2636)
2010Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Irajá Do Nascimento Filho, Agenor Furigo Junior, Marco LuccioAbstract:The aim of the present study was the assessment of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) using methyl and ethyl ricinoleate, ricinoleic acid and castor oil as precursors. The analysis of the volatile organic compounds was carried out using Head Space Solid Phase Micro-Extraction (HS - SPME). Factorial experimental design was used for investigating extraction conditions, verifying stirring rate (0-400 rpm), temperature (25-60 °C), extraction time (10-30 minutes), and sample volume (2-3 mL). The identification of volatile organic compounds was carried out by Gas Chromatography with Mass Spectrum Detector (GC/MSD). The conditions that resulted in maximum extraction were: 60 °C, 10 minutes extraction, no stirring, sample volume of 2.0 mL, and addition of saturated KCl (1:10 v/v). In the bio-production of volatile organic compounds the effect of stirring rate (120-200 rpm), temperature (23-33 °C), pH (4.08.0), precursor concentration (0.02-0.1%), mannitol (0-6%), and asparagine concentration (0-0.2%) was investigated. The bio-production at 28 °C, 160 rpm, pH 6,0 and with the addition of 0.02% ricinoleic acid to the medium yielded the highest production of VOCs, identified as 1,4-butanediol, 1,2,2-trimethylciclopropilamine, beta-ionone; 2,3-butanodione, pentanal, tetradecane, 2-isononenal, 4-octen-3-one, propanoic acid, and octadecane.
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Assessment of Cell Disruption and Carotenoids Extraction from Sporidiobolus salmonicolor (CBS 2636)
Food and Bioprocess Technology, 2009Co-Authors: Eunice Valduga, Alexsandra Valério, Pihetra Oliveira Tatsch, Helen Treichel, Agenor Furigo, Marco LuccioAbstract:The increasing demand for carotenoids by industries has drawn attention to their bio-production. Since pigments are intracellular, extraction steps are then needed after cell cultivation. In this work, different strategies for extraction of carotenoid pigments from Sporidiobolus salmonicolor (CBS 2636) were studied. Different solvents (dimethyl sulfoxide, petroleum ether, hexane, ethyl acetate, chloroform, and acetone), liquid N_2, and diatomaceous earth were used to disrupt the cell and thus release the intracellular carotenoids. The results of this study showed that when multiple solvents were used, a synergistic effect on the extent of carotenoids recovery was obtained. Maximum concentration of total carotenoids (913 μg/L) was obtained in the treatment using liquid N_2 and dimethyl sulfoxide to disrupt the cell, followed by the extraction with a solution of acetone/methanol (7:3, v / v ).
Agenor Furigo Junior - One of the best experts on this subject based on the ideXlab platform.
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Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) Microextração em Fase Sólida (MEFS) de compostos voláteis produzidos por Sporidiobolus salmonicolor (CBS 2636)
2010Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Irajá Do Nascimento Filho, Agenor Furigo Junior, Marco LuccioAbstract:The aim of the present study was the assessment of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) using methyl and ethyl ricinoleate, ricinoleic acid and castor oil as precursors. The analysis of the volatile organic compounds was carried out using Head Space Solid Phase Micro-Extraction (HS - SPME). Factorial experimental design was used for investigating extraction conditions, verifying stirring rate (0-400 rpm), temperature (25-60 °C), extraction time (10-30 minutes), and sample volume (2-3 mL). The identification of volatile organic compounds was carried out by Gas Chromatography with Mass Spectrum Detector (GC/MSD). The conditions that resulted in maximum extraction were: 60 °C, 10 minutes extraction, no stirring, sample volume of 2.0 mL, and addition of saturated KCl (1:10 v/v). In the bio-production of volatile organic compounds the effect of stirring rate (120-200 rpm), temperature (23-33 °C), pH (4.08.0), precursor concentration (0.02-0.1%), mannitol (0-6%), and asparagine concentration (0-0.2%) was investigated. The bio-production at 28 °C, 160 rpm, pH 6,0 and with the addition of 0.02% ricinoleic acid to the medium yielded the highest production of VOCs, identified as 1,4-butanediol, 1,2,2-trimethylciclopropilamine, beta-ionone; 2,3-butanodione, pentanal, tetradecane, 2-isononenal, 4-octen-3-one, propanoic acid, and octadecane.
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Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636)
Ciência e Tecnologia de Alimentos, 2010Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Irajá Do Nascimento Filho, Agenor Furigo Junior, Marco LuccioAbstract:O objetivo do presente estudo foi avaliar os compostos orgânicos volateis produzidos por Sporidiobolus salmonicolor (CBS 2636) utilizando metil e etil ricinoleato, acido ricinoleico e oleo de mamona como precursores. A analise dos compostos volateis produzidos foi conduzida por Micro-Extracao em Fase Solida (MEFS). A tecnica de planejamento experimental foi utilizada na avaliacao das condicoes de extracao, na qual se avaliaram a agitacao (0-400 rpm), a temperatura (25-60 oC), o tempo de extracao (10-30 minutos) e o volume da amostra (2-3 mL). A identificacao dos compostos foi realizada por Cromatografia a Gas com deteccao por Espectrometria de Massas (CG/EM). As condicoes que resultaram na maxima extracao foram: 60 oC, 10 minutos de extracao, sem agitacao, volume de amostra de 2,0 mL e adicao de solucao de KCl saturada (1:10 v/v). Na bioproducao dos compostos volateis, o efeito da agitacao (120-200 rpm), da temperatura (23-33 oC), do pH (4,0-8,0), da concentracao do precursor (0,02 a 0,1%), da concentracao de manitol (0 a 6%) e de asparagina (0 a 0,2%) foi avaliado. Na condicao de 28 oC, 160 rpm, pH 6,0 e com a adicao de 0,02% de acido ricinoleico ao meio, foi atingida a maxima producao dos compostos volateis, identificados como: 1,4-butanodiol, 1,2,2-trimetilciclopropilamina, beta-ionona; 2,3-butanodiona, pentanal, tetradecano, 2-isononenal, 4-octen-3-ona, acido propanoico e octadecano.
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Study of the bio-production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using pre-treated agro-industrial substrates
Journal of Chemical Technology & Biotechnology, 2008Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Marco Luccio, Agenor Furigo JuniorAbstract:BACKGROUND: The increasing industrial demand for carotenoids has aroused interest in their bio-production, and the need to reduce production costs has encouraged the use of low cost industrial substrates, such as agro-industrial residues. Thus the objective of this research was the bio-production of carotenoids by Sporidiobolus salmonicolor using agro-industrial substrates (corn steep liquor and sugarcane molasses), pre-treated with acids (sulphuric and phosphoric). RESULTS: Bio-production was carried out in an orbital shaker using a 10% (v/v) inoculum, incubation at 25 °C, and agitation at 180 rpm for 120 h in a non-illuminated environment. The carotenoids were recovered using liquid N2 combined with dimethylsulphoxide for cell rupture, and an acetone/methanol mixture (7:3 v/v) for extraction. CONCLUSION: The complete second-order design allowed for optimisation of the carotenoid concentration obtained from industrial substrates pre-treated with acids (sulphuric and phosphoric), obtaining a total carotenoid content of 541.5 µg L−1 using 10 g L−1 sugarcane molasses, 5 g L−1 corn steep liquor and 5 g L−1 yeast hydrolysate at 25 °C, with agitation at 180 rpm and an initial pH of 4.0. Copyright © 2008 Society of Chemical Industry
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Pré-tratamentos de melaço de cana-de-açúcar e água de maceração de milho para a bioprodução de carotenóides Pretreatment of sugarcane molasses and corn steep liquor for the production of carotenoids
Sociedade Brasileira de Química, 2007Co-Authors: Eunice Valduga, Alexsandra Valério, Helen Treichel, Marco Luccio, Rosângela Assis Jacques, Agenor Furigo JuniorAbstract:This work studied the pretreatment of sugarcane molasses (CM) and corn steep liquor (CS) for the production of carotenoids by Sporidiobolus salmonicolor (CBS 2636). The acid pretreatment removed less micronutrients than that with activated carbon and led to high removals of Cu and Mn. Reduction in optical density of the prepared medium and removal of glucose from it were 22% and 7% for CM and 95% and 38% for CS, respectively. Total carotenoids obtained with substrates pretreated with acids (541 mg/L) were higher than the results obtained when the medium was treated with activated carbon (208 mg/L)