The Experts below are selected from a list of 1545 Experts worldwide ranked by ideXlab platform
Juliana Helena Costa Smetana - One of the best experts on this subject based on the ideXlab platform.
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Fetuses from two FVB/NJ pregnant females injected with ZIKV at 5.5 days post coitum (dpc) and harvested at 10.5 dpc.
2017Co-Authors: Jose Xavier-neto, Murilo Carvalho, Bruno Dos Santos Pascoalino, Alisson Campos Cardoso, Ângela Maria Sousa Costa, Ana Helena Macedo Pereira, Luana Nunes Santos, Ângela Saito, Rafael Elias Marques, Juliana Helena Costa SmetanaAbstract:(A-D) All fetuses were alive at time of harvest. Lateral view of severely affected embryos. (C’-D’) Ventral views of embryos depicted in C-D, respectively. (E) Ventral view of an affected embryo, which was dead at harvest. (E’) Dorsal view of embryo depicted in E. Note the increased volume of the fourth ventricle and that the neural tube remains opened far below the otic capsule, at the level of the fifth somite, which is abnormally low for 9.5 dpc embryos. In all images: arrow, optic vesicle; asterisks, dorsal edemas; dashed circle, otic vesicle; dotted circle, cardiac situs inversus; Square Bracket, posterior hypotrophy; triangles in C, small, compacted somites; cross, dysraphia; 4V, fourth ventricle; LV, lateral ventricle. Scale bars, 0.5 mm.
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Fetuses from FVB/NJ pregnant female injected with ZIKV at 9.5 days post coitum (dpc).
2017Co-Authors: Jose Xavier-neto, Murilo Carvalho, Bruno Dos Santos Pascoalino, Alisson Campos Cardoso, Ângela Maria Sousa Costa, Ana Helena Macedo Pereira, Luana Nunes Santos, Ângela Saito, Rafael Elias Marques, Juliana Helena Costa SmetanaAbstract:Fetuses were harvested at 16.5 dpc. (A-H) Fetuses are displayed in an order of increased severity of developmental malformations. (I-M) Outwardly normal littermates. (N-P) Fetal phenotypes recorded immediately after harvesting and initial dissection. Prominent features of malformed embryos ranged from vascular rarefaction and pallor in the cephalic region (A, P) (Square Bracket), generalized edema (B, C) (arrows) to complete disruption of normal development (E-H). Note the intra amniotic hemorrhage in N, clouding of the amniotic fluid in O and the preferential pallor and/or vascular rarefaction in the cephalic region in P (Square Bracket). White arrowhead indicates abnormal forelimb postures suggestive of arthrogryposis. Scale bar, 2.0 mm.
Daniel R. Ambruso - One of the best experts on this subject based on the ideXlab platform.
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Changes in PLB-985 phox Proteins in Pre-Differentiated Cells Exposed to IFN-γ.
2015Co-Authors: Michael A. Ellison, Gail Thurman, Christy M. Gearheart, Ryan H. Seewald, Christopher C. Porter, Daniel R. AmbrusoAbstract:A; The western blots are from representative experiments and show the indicated Nox2 proteins in PLB-985 cells that were pre-differentiated for 72 hours in media containing 1.3% DMSO and were then exposed for 3 hours to nothing (lane 1) or 30ng/ml IFN-γ (lane2). Western blotting for GAPDH was done to establish controls for gel loading and transfer efficiency. For gp91phox the protein was detected over a broad range of molecular weights (indicated by the Square Bracket) due to glycosylation. B; The graphs show the average density of phox proteins measured in 4 experiments like the representative examples in A; the protein densities are expressed as a ratio to the corresponding GAPDH density. For densitometry of gp91phox the lanes were scanned to the extent indicated by the Square Bracket in the representative gp91phox blot. The error bars are +/- SEM. * indicates a significant difference (p
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PLB-985 phox Proteins in Cells Exposed to IFN-γ in the Presence or Absence of Differentiation.
2015Co-Authors: Michael A. Ellison, Gail Thurman, Christy M. Gearheart, Ryan H. Seewald, Christopher C. Porter, Daniel R. AmbrusoAbstract:A; The western blots are from representative experiments and show the indicated Nox2 proteins in PLB-985 cells that were cultured for 72 hours in the presence of media with, no additions (lane 1), 30ng/ml IFN-γ (lane 2), 1.3% DMSO (lane 3) or 1.3% DMSO plus 30ng/ml IFN-γ (lane 4). Blots were reprobed for GAPDH to establish controls for gel loading and transfer efficiency. For gp91phox the protein was detected over a broad range of molecular weights (indicated by the Square Bracket) due to glycosylation. B; The graphs show the average density of phox proteins measured in 4 experiments like the representative examples in A; the protein densities are expressed as a ratio to the corresponding GAPDH density. For densitometry of glycosylated and thus heterogeneously sized gp91phox, the lanes were scanned to the extent indicated by the Square Bracket in the representative gp91phox blot. The error bars are +/- SEM. For each protein, statistical differences with in the different cell culture conditions were confirmed by one way ANOVA and significance between each pair was then explored by post hoc-FDR analysis of paired t-tests. *, #, † and ‡ indicate significant difference from untreated cells (“no additions”), IFN-γ treatment, DMSO treatment and DMSO+IFN-γ treatment respectively (p
Yun-hong Tan - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic relationships within tribe Anemoneae based on the combination of nrITS and atpB-rbcL datasets.
2017Co-Authors: Nan Jiang, Zhuang Zhou, Jun-bo Yang, Shu-dong Zhang, Kai-yun Guan, Yun-hong TanAbstract:The topology is that of the majority rule consensus of BI tree. Bootstrap values of ML are presented under branches, and posterior probability of BI above branches. Topological incongruence between ML and BI trees is indicated by colored nodes/branches, and topology of BI tree shows by dash lines with posterior probability in Square Bracket under branches.
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Phylogenetic relationships within tribe Anemoneae based on the combination of five-plastid-gene dataset.
2017Co-Authors: Nan Jiang, Zhuang Zhou, Jun-bo Yang, Shu-dong Zhang, Kai-yun Guan, Yun-hong TanAbstract:The five plastid genes are matK, psbA-trnQ, rbcL, rpoB-trnC, and rps16. The topology is that of the majority rule consensus of ML tree. Bootstrap values of ML are presented above branches, and posterior probability of BI under branches. Topological incongruence between ML and BI trees is indicated by colored nodes/branches, and topology of BI tree shows by dashed lines with posterior probability in Square Bracket under branches.
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Phylogenetic relationships within tribe Anemoneae the combination of six-plastid-gene dataset.
2017Co-Authors: Nan Jiang, Zhuang Zhou, Jun-bo Yang, Shu-dong Zhang, Kai-yun Guan, Yun-hong TanAbstract:The six plastid genes are atpB-rbcL, matK, psbA-trnQ, rbcL, rpoB-trnC, and rps16. The topology is that of the majority rule consensus of ML tree. Bootstrap values of ML are presented above branches, and posterior probability of BI under branches. Topological incongruence between ML and BI trees is indicated by colored nodes/branches, and topology of BI tree shows by dash lines with posterior probability in Square Bracket under branches.
Jose Xavier-neto - One of the best experts on this subject based on the ideXlab platform.
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Fetuses from two FVB/NJ pregnant females injected with ZIKV at 5.5 days post coitum (dpc) and harvested at 10.5 dpc.
2017Co-Authors: Jose Xavier-neto, Murilo Carvalho, Bruno Dos Santos Pascoalino, Alisson Campos Cardoso, Ângela Maria Sousa Costa, Ana Helena Macedo Pereira, Luana Nunes Santos, Ângela Saito, Rafael Elias Marques, Juliana Helena Costa SmetanaAbstract:(A-D) All fetuses were alive at time of harvest. Lateral view of severely affected embryos. (C’-D’) Ventral views of embryos depicted in C-D, respectively. (E) Ventral view of an affected embryo, which was dead at harvest. (E’) Dorsal view of embryo depicted in E. Note the increased volume of the fourth ventricle and that the neural tube remains opened far below the otic capsule, at the level of the fifth somite, which is abnormally low for 9.5 dpc embryos. In all images: arrow, optic vesicle; asterisks, dorsal edemas; dashed circle, otic vesicle; dotted circle, cardiac situs inversus; Square Bracket, posterior hypotrophy; triangles in C, small, compacted somites; cross, dysraphia; 4V, fourth ventricle; LV, lateral ventricle. Scale bars, 0.5 mm.
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Fetuses from FVB/NJ pregnant female injected with ZIKV at 9.5 days post coitum (dpc).
2017Co-Authors: Jose Xavier-neto, Murilo Carvalho, Bruno Dos Santos Pascoalino, Alisson Campos Cardoso, Ângela Maria Sousa Costa, Ana Helena Macedo Pereira, Luana Nunes Santos, Ângela Saito, Rafael Elias Marques, Juliana Helena Costa SmetanaAbstract:Fetuses were harvested at 16.5 dpc. (A-H) Fetuses are displayed in an order of increased severity of developmental malformations. (I-M) Outwardly normal littermates. (N-P) Fetal phenotypes recorded immediately after harvesting and initial dissection. Prominent features of malformed embryos ranged from vascular rarefaction and pallor in the cephalic region (A, P) (Square Bracket), generalized edema (B, C) (arrows) to complete disruption of normal development (E-H). Note the intra amniotic hemorrhage in N, clouding of the amniotic fluid in O and the preferential pallor and/or vascular rarefaction in the cephalic region in P (Square Bracket). White arrowhead indicates abnormal forelimb postures suggestive of arthrogryposis. Scale bar, 2.0 mm.
Michael A. Ellison - One of the best experts on this subject based on the ideXlab platform.
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Changes in PLB-985 phox Proteins in Pre-Differentiated Cells Exposed to IFN-γ.
2015Co-Authors: Michael A. Ellison, Gail Thurman, Christy M. Gearheart, Ryan H. Seewald, Christopher C. Porter, Daniel R. AmbrusoAbstract:A; The western blots are from representative experiments and show the indicated Nox2 proteins in PLB-985 cells that were pre-differentiated for 72 hours in media containing 1.3% DMSO and were then exposed for 3 hours to nothing (lane 1) or 30ng/ml IFN-γ (lane2). Western blotting for GAPDH was done to establish controls for gel loading and transfer efficiency. For gp91phox the protein was detected over a broad range of molecular weights (indicated by the Square Bracket) due to glycosylation. B; The graphs show the average density of phox proteins measured in 4 experiments like the representative examples in A; the protein densities are expressed as a ratio to the corresponding GAPDH density. For densitometry of gp91phox the lanes were scanned to the extent indicated by the Square Bracket in the representative gp91phox blot. The error bars are +/- SEM. * indicates a significant difference (p
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PLB-985 phox Proteins in Cells Exposed to IFN-γ in the Presence or Absence of Differentiation.
2015Co-Authors: Michael A. Ellison, Gail Thurman, Christy M. Gearheart, Ryan H. Seewald, Christopher C. Porter, Daniel R. AmbrusoAbstract:A; The western blots are from representative experiments and show the indicated Nox2 proteins in PLB-985 cells that were cultured for 72 hours in the presence of media with, no additions (lane 1), 30ng/ml IFN-γ (lane 2), 1.3% DMSO (lane 3) or 1.3% DMSO plus 30ng/ml IFN-γ (lane 4). Blots were reprobed for GAPDH to establish controls for gel loading and transfer efficiency. For gp91phox the protein was detected over a broad range of molecular weights (indicated by the Square Bracket) due to glycosylation. B; The graphs show the average density of phox proteins measured in 4 experiments like the representative examples in A; the protein densities are expressed as a ratio to the corresponding GAPDH density. For densitometry of glycosylated and thus heterogeneously sized gp91phox, the lanes were scanned to the extent indicated by the Square Bracket in the representative gp91phox blot. The error bars are +/- SEM. For each protein, statistical differences with in the different cell culture conditions were confirmed by one way ANOVA and significance between each pair was then explored by post hoc-FDR analysis of paired t-tests. *, #, † and ‡ indicate significant difference from untreated cells (“no additions”), IFN-γ treatment, DMSO treatment and DMSO+IFN-γ treatment respectively (p