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R N C Guedes - One of the best experts on this subject based on the ideXlab platform.

  • biopesticide induced behavioral and morphological alterations in the Stingless Bee melipona quadrifasciata
    Environmental Toxicology and Chemistry, 2015
    Co-Authors: Wagner Faria Barbosa, Hudson Vaner Ventura Tome, Rodrigo Cupertino Bernardes, Maria Augusta L Siqueira, Guy Smagghe, R N C Guedes
    Abstract:

    Because of their natural origin, biopesticides are assumed to be less harmful to beneficial insects, including Bees, and therefore their use has Been widely encouraged for crop protection. There is little evidence, however, to support this ingrained notion of biopesticide safety to pollinators. Because larval exposure is still largely unexplored in ecotoxicology and risk assessment on Bees, an investigation was performed on the lethal and sublethal effects of a diet treated with 2 bioinsecticides, azadirachtin and spinosad, on the Stingless Bee, Melipona quadrifasciata, which is one of the most important pollinators in the Neotropics. Survival of Stingless Bee larvae was significantly compromised at doses above 210 ng a.i./Bee for azadirachtin and 114 ng a.i./Bee for spinosad. No sublethal effect was observed on larvae developmental time, but doses of both compounds negatively affected pupal body mass. Azadirachtin produced deformed pupae and adults as a result of its insect growth regulator properties, but spinosad was more harmful and produced greater numbers of deformed individuals. Only spinosad compromised walking activity of the adult workers at doses as low as 2.29 ng a.i./Bee, which is 1/5000 of the maximum field recommended rate. In conclusion, the results demonstrated that bioinsecticides can pose significant risks to native pollinators with lethal and sublethal effects; future investigations are needed on the likelihood of such effects under field conditions. Environ Toxicol Chem 2015;34:2149–2158. © 2015 SETAC

  • spinosad in the native Stingless Bee melipona quadrifasciata regrettable non target toxicity of a bioinsecticide
    Chemosphere, 2015
    Co-Authors: Hudson V V Tome, Wagner Faria Barbosa, Gustavo Ferreira Martins, R N C Guedes
    Abstract:

    Abstract The risks imposed by novel insecticides, mainly bioinsecticides, are largely unknown despite their increased use and their perceived environmental safety, which is based on their natural origin. Furthermore, unlike honeyBees, native pollinator species have received little attention. In the present study, the lethal and sublethal effects of the neonicotinoid imidacloprid and the bioinsecticide spinosad were assessed in the Stingless Bee species Melipona quadrifasciata, an important native pollinator in the Neotropical region. The adult Stingless Bee workers exhibited high oral insecticide susceptibility, with LD50s of 23.54 and 12.07 ng a.i./Bee for imidacloprid and spinosad, respectively. Imidacloprid also impaired worker respiration and overall group activity and flight, while spinosad significantly impaired only worker flight despite exhibiting higher oral toxicity to adult workers than imidacloprid. These findings indicate the hazardous nature not only of imidacloprid but also the bioinsecticide spinosad to adult workers of the native pollinator M. quadrifasciata. Therefore, bioinsecticides should not be exempted from risk assessment analysis due to their lethal and sublethal components.

  • imidacloprid induced impairment of mushroom bodies and behavior of the native Stingless Bee melipona quadrifasciata anthidioides
    PLOS ONE, 2012
    Co-Authors: Hudson V V Tome, Gustavo Ferreira Martins, Lucio Antonio De Oliveira Campos, Maria Augusta Pereira Lima, R N C Guedes
    Abstract:

    Declines in pollinator colonies represent a worldwide concern. The widespread use of agricultural pesticides is recognized as a potential cause of these declines. Previous studies have examined the effects of neonicotinoid insecticides such as imidacloprid on pollinator colonies, but these investigations have mainly focused on adult honey Bees. Native Stingless Bees (Hymenoptera: Apidae: Meliponinae) are key pollinators in neotropical areas and are threatened with extinction due to deforestation and pesticide use. Few studies have directly investigated the effects of pesticides on these pollinators. Furthermore, the existing impact studies did not address the issue of larval ingestion of contaminated pollen and nectar, which could potentially have dire consequences for the colony. Here, we assessed the effects of imidacloprid ingestion by Stingless Bee larvae on their survival, development, neuromorphology and adult walking behavior. Increasing doses of imidacloprid were added to the diet provided to individual worker larvae of the Stingless Bee Melipona quadrifasciata anthidioides throughout their development. Survival rates above 50% were only observed at insecticide doses lower than 0.0056 µg active ingredient (a.i.)/Bee. No sublethal effect on body mass or developmental time was observed in the surviving insects, but the pesticide treatment negatively affected the development of mushroom bodies in the brain and impaired the walking behavior of newly emerged adult workers. Therefore, Stingless Bee larvae are particularly susceptible to imidacloprid, as it caused both high mortality and sublethal effects that impaired brain development and compromised mobility at the young adult stage. These findings demonstrate the lethal effects of imidacloprid on native Stingless Bees and provide evidence of novel serious sublethal effects that may compromise colony survival. The ecological and economic importance of neotropical Stingless Bees as pollinators, their susceptibility to insecticides and the vulnerability of their larvae to insecticide exposure emphasize the importance of studying these species.

Fabiola Carina Biluca - One of the best experts on this subject based on the ideXlab platform.

  • investigation of phenolic compounds antioxidant and anti inflammatory activities in Stingless Bee honey meliponinae
    Food Research International, 2020
    Co-Authors: Fabiola Carina Biluca, Bibiana Silva, Thiago Caon, Eduarda Talita Bramorski Mohr, Guilherme Nicacio Vieira, Luciano Valdemiro Gonzaga, Luciano Vitali, Gustavo Amadeu Micke, Roseane Fett, Eduardo Monguilhott Dalmarco
    Abstract:

    Abstract The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight Stingless Bee honey types (Meliponinae) from southern Brazil. Stingless Bee honey did not show to be cytotoxic at the tested concentrations (1–100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages. Two honey samples showed the ability to increase the secretion of anti-inflammatory cytokine (interleukin-10), suggesting a significant anti-inflammatory effect. All these findings indicate that Stingless Bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.

  • Phenolic compounds, antioxidant capacity and bioaccessibility of minerals of Stingless Bee honey (Meliponinae)
    Journal of Food Composition and Analysis, 2017
    Co-Authors: Fabiola Carina Biluca, Luciano Valdemiro Gonzaga, Luciano Vitali, Gustavo Amadeu Micke, Francieli Braghini, Jefferson Santos De Gois, Mayara Schulz, Heloisa França Maltez, Eliseu Rodrigues, Daniel L. G. Borges
    Abstract:

    Abstract The phenolic composition, antioxidant capacity and bioaccessibility of the minerals of thirteen honey samples from nine different species of Stingless Bees (Meliponinae) were determined in this study. Twenty-six phenolic compounds were found. The major phenolic compounds were salicylic acid (8.02–94.8 μg 100 g1), p-coumaric acid (4.54–64.3 μg 100 g1), naringin (4.00–32.0 μg 100 g1) and taxifolin (12.0–1920 μg 100 g1). Moreover, the presence of mandelic acid, caffeic acid, chlorogenic acid, rosmarinic acid, aromadendrin, isoquercetrin, eriodictyol, vanillin, umbelliferone, syringaldehyde, sinapaldehyde and carnosol in Stingless Bee honeys was reported for the first time. The study also states that the abundant minerals in the samples were potassium (263–4980 μg g1), followed by calcium (88.7–138 μg g1), sodium (12.7–261 μg g1) and magnesium (25.9–231 μg g1). The estimation of the minerals bioaccessibility demonstrated high fractions (73.62–107.6% of the total concentrations). Stingless Bee honey has considerable concentrations of phenolic compounds and macro minerals (K, Ca, Na and Mg) as well as a related antioxidant capacity, suggesting a source of natural antioxidants.

  • physicochemical profiles minerals and bioactive compounds of Stingless Bee honey meliponinae
    Journal of Food Composition and Analysis, 2016
    Co-Authors: Fabiola Carina Biluca, Luciano Valdemiro Gonzaga, Ana Carolina Oliveira Costa, Francieli Braghini, Roseane Fett
    Abstract:

    Abstract This study investigates the physicochemical properties, majority minerals, total phenolic compounds and antioxidant activity of Stingless Bee honey. Thirty-three samples from ten species were analyzed. The results show moisture as 23.1–43.5% (w/w), the electrical conductivity as 0.150–1.34 mS cm −1 , the free acid as 16.2–139 mEq kg −1 , the pH as 3.33–6.56, the diastase activity as 4.34–49.6 in Gothe units, the insoluble solids as 55.2–76.1°Brix, the carbohydrates as 48.6–70.5% (w/w) sucrose and the 5-hydroxymethylfurfural (5-HMF) was below the limit of quantitation (LOQ). The most abundant element in the samples was potassium followed by calcium sodium, magnesium and manganese. Stingless Bee honeys possess relevant amounts of phenolic compounds and antioxidant activity, suggesting a source of natural antioxidants. The results show that there is great variability in the composition of honey from Stingless Bees of different species, predominantly flowering and geographical origin. However, it was not possible to identify the real factor influencing its features.

  • 5 hmf and carbohydrates content in Stingless Bee honey by ce before and after thermal treatment
    Food Chemistry, 2014
    Co-Authors: Fabiola Carina Biluca, Luciano Valdemiro Gonzaga, Fabiana Della Betta, Gabriela Pirassol De Oliveira, Lais Morilla Pereira, Ana Carolina Oliveira Costa, Roseane Fett
    Abstract:

    This study aimed to assess 5-hydroximethylfurfural and carbohydrates (fructose, glucose, and sucrose) in 13 Stingless Bee honey samples before and after thermal treatment using a capillary electrophoresis method. The methods were validated for the parameters of linearity, matrix effects, precision, and accuracy. A factorial design was implemented to determine optimal thermal treatment conditions and then verify the postprocedural 5-HMF formation, but once 5-HMF were thermal treatment, Apis mellifera honey presented higher 5-HMF content than Stingless Bee honey. Results suggest that a high temperature related to briefer thermal treatment could be an efficient way to extend shelf life without affecting 5-HMF content in Stingless Bee honey.

Katherine A Hammer - One of the best experts on this subject based on the ideXlab platform.

  • antimicrobial activity of honey from the Stingless Bee trigona carbonaria determined by agar diffusion agar dilution broth microdilution and time kill methodology
    Journal of Applied Microbiology, 2010
    Co-Authors: K L Boorn, Y Y Khor, E Sweetman, Tim A Heard, Katherine A Hammer
    Abstract:

    AIMS: The aim of this study was to determine the spectrum of antimicrobial activity of 11 samples of Stingless Bee honey compared to medicinal, table and artificial honeys. METHODS AND RESULTS: Activity was assessed by agar diffusion, agar dilution, broth microdilution and time-kill viability assays. By agar dilution, minimum inhibitory concentration (MIC) ranges were 4% to >10% (w/v) for Gram-positive bacteria, 6% to >16% (w/v) for Gram-negative bacteria and 6% to >10% (w/v) for Candida spp. By broth microdilution, all organisms with the exception of Candida albicans and Candida glabrata were inhibited at 3 log for Pseudomonas aeruginosa and <1 log for C. albicans. Similar treatment with each control honey resulted in decreases of <1 log for all organisms. CONCLUSIONS: Stingless Bee honey has broad-spectrum antibacterial activity although activity against Candida was limited. Stingless Bee honey samples varied in activity and the basis for this remains to be determined. SIGNIFICANCE AND IMPACT OF THE STUDY: Stingless Bee honey had similar activity to medicinal honey and may therefore have a role as a medicinal agent.

  • antimicrobial activity of honey from the Stingless Bee trigona carbonaria determined by agar diffusion agar dilution broth microdilution and time kill methodology
    Journal of Applied Microbiology, 2010
    Co-Authors: K L Boorn, Y Y Khor, E Sweetman, Tim A Heard, F Tan, Katherine A Hammer
    Abstract:

    Aims:  The aim of this study was to determine the spectrum of antimicrobial activity of 11 samples of Stingless Bee honey compared to medicinal, table and artificial honeys. Methods and Results:  Activity was assessed by agar diffusion, agar dilution, broth microdilution and time-kill viability assays. By agar dilution, minimum inhibitory concentration (MIC) ranges were 4% to >10% (w/v) for Gram-positive bacteria, 6% to >16% (w/v) for Gram-negative bacteria and 6% to >10% (w/v) for Candida spp. By broth microdilution, all organisms with the exception of Candida albicans and Candida glabrata were inhibited at ≤32% (w/v). Geometric MIC (w/v) means for Stingless Bee honeys ranged from 7·1% to 16·0% and were 11·7% for medicinal honey and 26·5% for table honey. Treatment of organisms with 20% (w/v) Stingless Bee honey for 60 min resulted in decreases of 1–3 log for Staphylococcus aureus, >3 log for Pseudomonas aeruginosa and <1 log for C. albicans. Similar treatment with each control honey resulted in decreases of <1 log for all organisms. Conclusions:  Stingless Bee honey has broad-spectrum antibacterial activity although activity against Candida was limited. Stingless Bee honey samples varied in activity and the basis for this remains to be determined. Significance and Impact of the Study:  Stingless Bee honey had similar activity to medicinal honey and may therefore have a role as a medicinal agent.

Roseane Fett - One of the best experts on this subject based on the ideXlab platform.

  • investigation of phenolic compounds antioxidant and anti inflammatory activities in Stingless Bee honey meliponinae
    Food Research International, 2020
    Co-Authors: Fabiola Carina Biluca, Bibiana Silva, Thiago Caon, Eduarda Talita Bramorski Mohr, Guilherme Nicacio Vieira, Luciano Valdemiro Gonzaga, Luciano Vitali, Gustavo Amadeu Micke, Roseane Fett, Eduardo Monguilhott Dalmarco
    Abstract:

    Abstract The objective of this study was to investigate and quantify the composition phenolic, reducing capacity, the free radical scavenging activity, as well as, the anti-inflammatory effect evaluated against lipopolysaccharides-stimulated RAW264.7 macrophages through modulation of inflammatory mediators, in eight Stingless Bee honey types (Meliponinae) from southern Brazil. Stingless Bee honey did not show to be cytotoxic at the tested concentrations (1–100 µM) and also reduced nitric oxide and the secretion of the pro-inflammatory cytokine in the inflamed macrophages. Two honey samples showed the ability to increase the secretion of anti-inflammatory cytokine (interleukin-10), suggesting a significant anti-inflammatory effect. All these findings indicate that Stingless Bee honey could be an important source of natural compounds presenting anti-inflammatory and antioxidant effect, which could would provide health benefits when included in the diet.

  • physicochemical profiles minerals and bioactive compounds of Stingless Bee honey meliponinae
    Journal of Food Composition and Analysis, 2016
    Co-Authors: Fabiola Carina Biluca, Luciano Valdemiro Gonzaga, Ana Carolina Oliveira Costa, Francieli Braghini, Roseane Fett
    Abstract:

    Abstract This study investigates the physicochemical properties, majority minerals, total phenolic compounds and antioxidant activity of Stingless Bee honey. Thirty-three samples from ten species were analyzed. The results show moisture as 23.1–43.5% (w/w), the electrical conductivity as 0.150–1.34 mS cm −1 , the free acid as 16.2–139 mEq kg −1 , the pH as 3.33–6.56, the diastase activity as 4.34–49.6 in Gothe units, the insoluble solids as 55.2–76.1°Brix, the carbohydrates as 48.6–70.5% (w/w) sucrose and the 5-hydroxymethylfurfural (5-HMF) was below the limit of quantitation (LOQ). The most abundant element in the samples was potassium followed by calcium sodium, magnesium and manganese. Stingless Bee honeys possess relevant amounts of phenolic compounds and antioxidant activity, suggesting a source of natural antioxidants. The results show that there is great variability in the composition of honey from Stingless Bees of different species, predominantly flowering and geographical origin. However, it was not possible to identify the real factor influencing its features.

  • 5 hmf and carbohydrates content in Stingless Bee honey by ce before and after thermal treatment
    Food Chemistry, 2014
    Co-Authors: Fabiola Carina Biluca, Luciano Valdemiro Gonzaga, Fabiana Della Betta, Gabriela Pirassol De Oliveira, Lais Morilla Pereira, Ana Carolina Oliveira Costa, Roseane Fett
    Abstract:

    This study aimed to assess 5-hydroximethylfurfural and carbohydrates (fructose, glucose, and sucrose) in 13 Stingless Bee honey samples before and after thermal treatment using a capillary electrophoresis method. The methods were validated for the parameters of linearity, matrix effects, precision, and accuracy. A factorial design was implemented to determine optimal thermal treatment conditions and then verify the postprocedural 5-HMF formation, but once 5-HMF were thermal treatment, Apis mellifera honey presented higher 5-HMF content than Stingless Bee honey. Results suggest that a high temperature related to briefer thermal treatment could be an efficient way to extend shelf life without affecting 5-HMF content in Stingless Bee honey.

Tim A Heard - One of the best experts on this subject based on the ideXlab platform.

  • the australian Stingless Bee industry a follow up survey one decade on
    Journal of Apicultural Research, 2013
    Co-Authors: Megan Halcroft, Tim A Heard, Robert Spoonerhart, Anthony M Haigh, Anne E Dollin
    Abstract:

    SummaryIn 2010, an online survey was conducted to assess the current status of the Australian Stingless Bee industry and its recent development. This was a follow-up survey conducted approximately one decade after the first study, by Heard and Dollin in 1998/99. It showed that the Australian industry had grown over the past ten or so years but is still underdeveloped. There was a 2.5-fold increase in the number of Bee keepers and a 3.5 fold increase in the number of domesticated colonies. Seventy-eight percent of Bee keepers were hobbyists, 54% of whom owned only one colony. Most colonies were kept in suburban areas. Two species, Tetragonula carbonaria and Austroplebeia australis, dominated the relatively short list of species kept. There was a high demand for Australian Stingless Bee colonies and their honey, but with less than 250 Bee keepers currently propagating colonies, and many of them on a small scale, it is difficult to meet this demand. Pollination services were provided by less than 4% of the m...

  • antimicrobial activity of honey from the Stingless Bee trigona carbonaria determined by agar diffusion agar dilution broth microdilution and time kill methodology
    Journal of Applied Microbiology, 2010
    Co-Authors: K L Boorn, Y Y Khor, E Sweetman, Tim A Heard, Katherine A Hammer
    Abstract:

    AIMS: The aim of this study was to determine the spectrum of antimicrobial activity of 11 samples of Stingless Bee honey compared to medicinal, table and artificial honeys. METHODS AND RESULTS: Activity was assessed by agar diffusion, agar dilution, broth microdilution and time-kill viability assays. By agar dilution, minimum inhibitory concentration (MIC) ranges were 4% to >10% (w/v) for Gram-positive bacteria, 6% to >16% (w/v) for Gram-negative bacteria and 6% to >10% (w/v) for Candida spp. By broth microdilution, all organisms with the exception of Candida albicans and Candida glabrata were inhibited at 3 log for Pseudomonas aeruginosa and <1 log for C. albicans. Similar treatment with each control honey resulted in decreases of <1 log for all organisms. CONCLUSIONS: Stingless Bee honey has broad-spectrum antibacterial activity although activity against Candida was limited. Stingless Bee honey samples varied in activity and the basis for this remains to be determined. SIGNIFICANCE AND IMPACT OF THE STUDY: Stingless Bee honey had similar activity to medicinal honey and may therefore have a role as a medicinal agent.

  • antimicrobial activity of honey from the Stingless Bee trigona carbonaria determined by agar diffusion agar dilution broth microdilution and time kill methodology
    Journal of Applied Microbiology, 2010
    Co-Authors: K L Boorn, Y Y Khor, E Sweetman, Tim A Heard, F Tan, Katherine A Hammer
    Abstract:

    Aims:  The aim of this study was to determine the spectrum of antimicrobial activity of 11 samples of Stingless Bee honey compared to medicinal, table and artificial honeys. Methods and Results:  Activity was assessed by agar diffusion, agar dilution, broth microdilution and time-kill viability assays. By agar dilution, minimum inhibitory concentration (MIC) ranges were 4% to >10% (w/v) for Gram-positive bacteria, 6% to >16% (w/v) for Gram-negative bacteria and 6% to >10% (w/v) for Candida spp. By broth microdilution, all organisms with the exception of Candida albicans and Candida glabrata were inhibited at ≤32% (w/v). Geometric MIC (w/v) means for Stingless Bee honeys ranged from 7·1% to 16·0% and were 11·7% for medicinal honey and 26·5% for table honey. Treatment of organisms with 20% (w/v) Stingless Bee honey for 60 min resulted in decreases of 1–3 log for Staphylococcus aureus, >3 log for Pseudomonas aeruginosa and <1 log for C. albicans. Similar treatment with each control honey resulted in decreases of <1 log for all organisms. Conclusions:  Stingless Bee honey has broad-spectrum antibacterial activity although activity against Candida was limited. Stingless Bee honey samples varied in activity and the basis for this remains to be determined. Significance and Impact of the Study:  Stingless Bee honey had similar activity to medicinal honey and may therefore have a role as a medicinal agent.

  • No worker reproduction in the Australian Stingless Bee Trigona carbonaria Smith (Hymenoptera, Apidae)
    Insectes Sociaux, 2007
    Co-Authors: Ros Gloag, Tim A Heard, Madeleine Beekman, Benjamin P. Oldroyd
    Abstract:

    Brood of the Australian Stingless Bee Trigona carbonaria was investigated using microsatellites to determine the origin of males. Genotypes of over 1800 males sampled from ten unrelated colonies were consistent with the hypothesis that the resident queen was the sole mother of the males and that workers, either natal or non-natal (parasitic), did not contribute to the production of males. Dissections of 300 workers from a total of four colonies showed that ovaries are present but not activated.

  • Composition of Stingless Bee honey: Setting quality standards
    Interciencia, 2006
    Co-Authors: Bruno Henrique Sardinha De Souza, Tim A Heard, David W. Roubik, Ortrud Monika Barth, Eunice Enríquez, Carlos Alfredo Lopes De Carvalho, Luís Carlos Marchini, Jean Locatelli, Livia Persano-oddo, Ligia Bicudo De Almeida-muradian
    Abstract:

    SUMMARY Compositional data from 152 Stingless Bee (Meliponini) honey samples were compiled from studies since 1964, and evaluated to propose a quality standard for this product. Since Stingless Bee honey has a different composition than Apis mellifera honey, some physicochemical parameters are presented according to Stingless Bee species. The entomological origin of the honey was known for 17 species of Meliponini from Brazil, one from Costa Rica, six from Mexico, 27 from Panama, one from Surinam, two from Trinidad & Tobago, and seven from Venezuela, most from the genus Melipona. The results varied as follows: moisture (19.9-41.9g/100g), pH (3.15-4.66), free acidity (5.9-109.0meq/ Kg), ash (0.01-1.18g/100g), diastase activity (0.9-23.0DN), electrical conductivity (0.49-8.77mS/cm), HMF (0.4-78.4mg/Kg), invertase activity (19.8-90.1IU), nitrogen (14.34-144.00mg/100g), reducing sugars (58.0-75.7g/100g) and sucrose (1.1-4.8g/100g). Moisture content of Stingless Bee honey is generally higher than