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Xiao-rong Chang - One of the best experts on this subject based on the ideXlab platform.
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1h nmr based investigation of metabolic response to electro acupuncture stimulation
Scientific Reports, 2017Co-Authors: Kian Kai Cheng, Guiping Shen, Jingjing Xu, Huan Zhong, Xiao-rong Chang, Jiyang DongAbstract:Acupuncture is a traditional Chinese medicine therapy that has been found useful for treating various diseases. The treatments involve the insertion of fine needles at acupoints along specific Meridians (Meridian specificity). This study aims to investigate the metabolic basis of Meridian specificity using proton nuclear magnetic resonance (1H NMR)-based metabolomics. Electro-acupuncture (EA) stimulations were performed at acupoints of either Stomach Meridian of Foot-Yangming (SMFY) or Gallbladder Meridian of Foot-Shaoyang (GMFS) in healthy male Sprague Dawley (SD) rats. 1H-NMR spectra datasets of serum, urine, cortex, and Stomach tissue extracts from the rats were analysed by multivariate statistical analysis to investigate metabolic perturbations due to EA treatments at different Meridians. EA treatment on either the SMFY or GMFS acupoints induced significant variations in 31 metabolites, e.g., amino acids, organic acids, choline esters and glucose. Moreover, a few Meridian-specific metabolic changes were found for EA stimulations on the SMFY or GMFS acupoints. Our study demonstrated significant metabolic differences in response to EA stimulations on acupoints of SMFY and GMFS Meridians. These results validate the hypothesis that Meridian specificity in acupuncture is detectable in the metabolome and demonstrate the feasibility and effectiveness of a metabolomics approach in understanding the mechanism of acupuncture.
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action mechanism of electroacupuncture at Stomach Meridian acupoints for oxidative damage in rats with gastric ulcer
Chinese acupuncture & moxibustion, 2016Co-Authors: Zong-bao Yang, Ya-dong Wang, Qiong Liu, Mi Liu, Huijuan Chen, Xiao-rong ChangAbstract:OBJECTIVE To observe the effects of electroacupuncture (EA) at Stomach Meridian acupoints on expression of oxidation damage factors in serum and gastric mucosal cells in rats with gastric ulcer, and to explore the mechanism of EA at Stomach Meridian acupoints for oxidative damage in rats with gastric ulcer. METHODS Forty clean-grade SD rats were randomly divided into a normal group, a model group, a Stomach Meridian group and a gallbladder Meridian group, ten rats in each one. Except the normal group, rats in the remaining groups were applied the restraint-cold stress method to establish the model of gastric ulcer. Rats in the normal group and model group received no treatment; rats in the Stomach Meridian group were treated with EA at "Liangmen" (ST 21) and "Zusanli" (ST 36); rats in the gallbladder Meridian group were treated with EA at "Riyue" (GB 24) and "Yanglingquan" (GB 34). The EA was given for 30 min, once a day for 7 days totally. The change of gastric mucosal morphology was observed by routine light microscope; enzyme linked immunosorbent assay was used to detect the expressions of malondialdehyde (MDA), glutathione peroxidase (GSH-px) and tumor necrosis factor-α (TNF-α), interleukin-2(IL-2), interleukin-6(IL-6) in serum and gastric mucosal cells of rats. RESULTS After treatment, compared with the model group, the gastric mucosal damage index was decreased in the Stomach Meridian group and gallbladder Meridian group (both P<0.05), the expressions of MDA, TNF-α, IL-2 and IL-6 in serum and gastric mucosal cells were significantly decreased in the Stomach Meridian group (all P<0.01), but the contents of GSH-Px in serum and gastric mucosal cells were increased significantly (both P<0.01). Compared with the gallbladder Meridian group, the gastric mucosal damage index as well as the expressions of MDA,TNF-α, IL-2 and IL-6 in serum and gastric mucosal cells were significantly decreased in the Stomach Meridian group rats (P<0.05, P<0.01), and the contents of GSH-px in serum and gastric mucosal cells were increased significantly (both P<0.01). CONCLUSIONS EA at Stomach Meridian acupoints is likely to inhibit the expressions of oxidative damage factors to promote the repair of gastric mucosal injury, which indicates the correlation between Meridians and zang-fu.
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neuron discharge and c fos expression in the nucleus of the solitary tract following electroacupuncture at acupoints of the yangming Stomach Meridian of foot
Journal of Acupuncture and Meridian Studies, 2013Co-Authors: Jie Yan, Chao Wang, Jianhua Liu, Xiao-rong ChangAbstract:The nucleus of the solitary tract (nucleus tractus solitarii; NTS) is a primary center for both visceral afferents and somatic afferents. Previous experiments have demonstrated that the NTS is closely connected to the Stomach and acupoints in the Yangming Stomach Meridian of Foot (ST Meridian). In this study, extracellular recording and immunochemistry methods were used to analyze the discharge of neurons and c-Fos protein expression in the NTS following acupuncture at different acupoints and a nonacupoint. A total of 104 discharging neurons were detected in the NTS of 52 rats, of which 86 provided complete data. After acupuncture at Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint, the neuron response rate in the NTS was 65.12%, 51.16%, 46.51%, 34.88% and 31.40% respectively. For neuron response rate, there was a significant difference among Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint (p 0.05). The experiments demonstrated that the afferent convergence in NTS are different by body surface points stimulus, which suggests that the NTS might be a primary center in the central nervous system receiving acupoints stimulus from the ST Meridian.
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Randomised clinical trial: an assessment of acupuncture on specific Meridian or specific acupoint vs. sham acupuncture for treating functional dyspepsia.
Alimentary Pharmacology & Therapeutics, 2012Co-Authors: Fanrong Liang, Xiao-ping Tian, Guo-jie Sun, Hui Zheng, Jie Yan, Xiao-rong Chang, Ling ZhaoAbstract:SummaryBackground Functional dyspepsia (FD) is a common disease without an established optimal treatment. Aim To determine (i) the effect of acupuncture in relieving FD symptoms and improving life quality; (ii) the effect difference between acupoint and non-acupoint; and (iii) the effect difference among different acupoints. Methods A total of 712 eligible patients were included and randomly assigned to six groups (Group A: specific acupoints of the Stomach Meridian; Group B: non-specific acupoints of the Stomach Meridian; Group C: specific acupoints of alarm and transport points; Group D: specific acupoints of the gallbladder Meridian; Group E: sham acupuncture of non-acupoints; and Group F: itopride). A treatment period of 4 weeks (continuous five sessions per week), and a follow-up period of 12 weeks were arranged. The outcomes were the (i) patients’ response, (ii) symptoms improvement measured using the Symptom Index of Dyspepsia and (iii) quality-of-life improvement based on Nepean Dyspepsia Index. Results All groups had an improvement in dyspepsia symptoms and the QoL at the end of treatment, and the improvement was sustained for 4 weeks and 12 weeks. The overall response rate was significantly higher in acupuncture group A (70.69%), and lower in sham acupuncture group (34.75%), compared with itopride and other acupuncture groups. Similarly, the difference in symptoms and QoL improvement was significant between group A and the other acupuncture groups. Conclusions Acupuncture is effective in the treatment of functional dyspepsia, and is superior to non-acupoint puncture. The benefit of acupuncture relies on acupoint specificity.
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expressions of epidermal growth factor receptor signaling substances in gastric mucosal cells influenced by serum derived from rats treated with electroacupuncture at Stomach Meridian acupoints
Journal of Chinese Integrative Medicine, 2007Co-Authors: Jie Yan, Zong-bao Yang, Xiao-rong Chang, Yaping Lin, Yan ZhongAbstract:OBJECTIVE: To study the effects of serum derived from rats treated with electroacupuncture at Stomach Meridian acupoints on the expressions of epidermal growth factor receptor (EGFR) signaling substances phospholipase C gamma-1 (PLC gamma-1), protein kinase C (PKC) and c-myc in gastric mucosal cells. METHODS: Sixty rats were randomly divided into normal group, Stomach Meridian group, gallbladder Meridian group, Stomach Meridian plus PD153035 group and gallbladder Meridian plus PD153035 group. Water-immersion and restrained stress methods were adopted for inducing gastric mucosal injury in the rats. Gastric mucosal cells were separated by using pronase digestion method, and incubated by PD153035, a EGFR inhibitor, and 100 ml/L serum. The expression of PLC gamma-1 in the gastric mucosal cells was tested by enzyme linked-immunosorbent assay (ELISA), while the expression of PKC by isotope incorporate assay and the expression of c-myc by reverse transcription polymerase chain reaction assay (RT-PCR). RESULTS: In gastric mucosal cells, weak expressions of PLC gamma-1, PKC and c-myc were seen in the normal group, and relatively strong expressions of PLC gamma-1, PKC and c-myc were seen in the Stomach Meridian group and the gallbladder Meridian group, among which, the expressions of PLC gamma-1, PKC and c-myc in the Stomach Meridian group were the strongest, and there was a significant difference between the Stomach Meridian group and the gallbladder Meridian group (P<0.01). Relative weak expressions of PLC gamma-1, PKC and c-myc were seen in the Stomach Meridian plus PD153035 group and the gallbladder Meridian plus PD153035 group, and there was a significant difference between the Stomach Meridian group and the Stomach Meridian plus PD153035 group (P<0.01). CONCLUSIONS: The serum derived from the rats treated with electroacupuncture at Stomach Meridian acupoints can activate the EGFR singling pathway, and this provides an evidence for the theory of "relative particularity between Meridians and viscera" in traditional Chinese medicine.
David D. Kim - One of the best experts on this subject based on the ideXlab platform.
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acupuncture reduces experimental renovascular hypertension through mechanisms involving nitric oxide synthases
Microcirculation, 2006Co-Authors: David D. Kim, Arnaldo Michel Pica, Ricardo G Duran, Walter N DuranAbstract:Objective: To test the hypothesis that acupuncture on Stomach 36 point (ST-36) reduces hypertension by activating nitric oxide synthase signaling mechanisms. Methods: The authors used the two-kidney, one-clip renal hypertension (2K1C) hamster model with electroacupuncture treatment. Results: Thirty-minute daily electroacupuncture treatment for 5 days reduced mean arterial pressure from 160.0 ± 7.6 to 128.0 ± 4.3 mmHg (mean ± SEM), compared to 115.0 ± 7.2 mmHg in sham-operated hamsters. Electroacupuncture increased periarteriolar NO concentration from 309.0 ± 21.7 nM to 417.9 ± 20.9 nM in the 2K1C hamster cheek pouch microcirculation when measured with NO-sensitive microelectrodes. Hypertension reduced endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) proteins relative to the sham-operated control, as measured by Western blotting. Electroacupuncture prevented the reduction of eNOS and nNOS associated with hypertension and showed even higher eNOS and nNOS expressions than sham-operated control in Stomach and cheek pouch tissues, which are on the Stomach Meridian. Analysis of liver tissue, a nonStomach-Meridian organ, indicated that electroacupuncture did not have a significant benefit in terms of enhanced expressions of eNOS and nNOS in the treated 2K1C hypertensive group.
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electroacupuncture on Stomach 36 point st 36 reduces hypertension through endothelial nitric oxide synthase enos activity in the Stomach Meridian
The FASEB Journal, 2006Co-Authors: Arnaldo Michel Pica, Ricardo G Duran, Walter N Duran, David D. KimAbstract:Acupuncture has been used in Asia to treat hypertension for a long time. We hypothesized that acupuncture on ST-36 activates eNOS signaling mechanisms to reduce hypertension. We tested the hypothes...
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Acupuncture reduces experimental renovascular hypertension through mechanisms involving nitric oxide synthases
2006Co-Authors: David D. Kim, Arnaldo M. Pica, Ricardo G. DuráAbstract:Objective: To test the hypothesis that acupuncture on Stomach 36 point (ST-36) reduces hypertension by activating nitric oxide synthase signaling mechanisms. Methods: The authors used the two-kidney, one-clip renal hypertension (2K1C) hamster model with electroacupuncture treatment. Results: Thirty-minute daily electroacupuncture treatment for 5 days reduced mean arterial pres-sure from 160.0 ± 7.6 to 128.0 ± 4.3 mmHg (mean ± SEM), compared to 115.0 ± 7.2 mmHg in sham-operated hamsters. Electroacupuncture increased periarteriolar NO concentration from 309.0 ± 21.7 nM to 417.9 ± 20.9 nM in the 2K1C hamster cheek pouch microcirculation when measured with NO-sensitive microelectrodes. Hypertension reduced endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) proteins relative to the sham-operated control, as measured by Western blotting. Electroacupuncture prevented the reduction of eNOS and nNOS associated with hypertension and showed even higher eNOS and nNOS expressions than sham-operated control in Stomach and cheek pouch tissues, which are on the Stomach Meridian. Analysis of liver tissue, a non-Stomach-Meridian organ, indicated that electroacupuncture did not have a significant benefit in terms of enhanced expressions of eNOS and nNOS in the treated 2K1C hypertensive group. Conclusions: Activation of eNOS and nNOS is one of the mechanisms through which ST-36 elec-troacupuncture reduces blood pressure; this reduction works through the Stomach Meridian. Microcirculation (2006) 13, 577–585. doi:10.1080/1073968060088521
Jie Yan - One of the best experts on this subject based on the ideXlab platform.
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neuron discharge and c fos expression in the nucleus of the solitary tract following electroacupuncture at acupoints of the yangming Stomach Meridian of foot
Journal of Acupuncture and Meridian Studies, 2013Co-Authors: Jie Yan, Chao Wang, Jianhua Liu, Xiao-rong ChangAbstract:The nucleus of the solitary tract (nucleus tractus solitarii; NTS) is a primary center for both visceral afferents and somatic afferents. Previous experiments have demonstrated that the NTS is closely connected to the Stomach and acupoints in the Yangming Stomach Meridian of Foot (ST Meridian). In this study, extracellular recording and immunochemistry methods were used to analyze the discharge of neurons and c-Fos protein expression in the NTS following acupuncture at different acupoints and a nonacupoint. A total of 104 discharging neurons were detected in the NTS of 52 rats, of which 86 provided complete data. After acupuncture at Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint, the neuron response rate in the NTS was 65.12%, 51.16%, 46.51%, 34.88% and 31.40% respectively. For neuron response rate, there was a significant difference among Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint (p 0.05). The experiments demonstrated that the afferent convergence in NTS are different by body surface points stimulus, which suggests that the NTS might be a primary center in the central nervous system receiving acupoints stimulus from the ST Meridian.
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Randomised clinical trial: an assessment of acupuncture on specific Meridian or specific acupoint vs. sham acupuncture for treating functional dyspepsia.
Alimentary Pharmacology & Therapeutics, 2012Co-Authors: Fanrong Liang, Xiao-ping Tian, Guo-jie Sun, Hui Zheng, Jie Yan, Xiao-rong Chang, Ling ZhaoAbstract:SummaryBackground Functional dyspepsia (FD) is a common disease without an established optimal treatment. Aim To determine (i) the effect of acupuncture in relieving FD symptoms and improving life quality; (ii) the effect difference between acupoint and non-acupoint; and (iii) the effect difference among different acupoints. Methods A total of 712 eligible patients were included and randomly assigned to six groups (Group A: specific acupoints of the Stomach Meridian; Group B: non-specific acupoints of the Stomach Meridian; Group C: specific acupoints of alarm and transport points; Group D: specific acupoints of the gallbladder Meridian; Group E: sham acupuncture of non-acupoints; and Group F: itopride). A treatment period of 4 weeks (continuous five sessions per week), and a follow-up period of 12 weeks were arranged. The outcomes were the (i) patients’ response, (ii) symptoms improvement measured using the Symptom Index of Dyspepsia and (iii) quality-of-life improvement based on Nepean Dyspepsia Index. Results All groups had an improvement in dyspepsia symptoms and the QoL at the end of treatment, and the improvement was sustained for 4 weeks and 12 weeks. The overall response rate was significantly higher in acupuncture group A (70.69%), and lower in sham acupuncture group (34.75%), compared with itopride and other acupuncture groups. Similarly, the difference in symptoms and QoL improvement was significant between group A and the other acupuncture groups. Conclusions Acupuncture is effective in the treatment of functional dyspepsia, and is superior to non-acupoint puncture. The benefit of acupuncture relies on acupoint specificity.
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research status and perspective on correlation between Stomach Meridian points and Stomach
Journal of Acupuncture and Tuina Science, 2008Co-Authors: Yingjin Zhang, Jie YanAbstract:This article expounds the action mechanism on correlation between Stomach Meridians points and Stomach as well as its perspective from myriad ancient and modern literature.
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expressions of epidermal growth factor receptor signaling substances in gastric mucosal cells influenced by serum derived from rats treated with electroacupuncture at Stomach Meridian acupoints
Journal of Chinese Integrative Medicine, 2007Co-Authors: Jie Yan, Zong-bao Yang, Xiao-rong Chang, Yaping Lin, Yan ZhongAbstract:OBJECTIVE: To study the effects of serum derived from rats treated with electroacupuncture at Stomach Meridian acupoints on the expressions of epidermal growth factor receptor (EGFR) signaling substances phospholipase C gamma-1 (PLC gamma-1), protein kinase C (PKC) and c-myc in gastric mucosal cells. METHODS: Sixty rats were randomly divided into normal group, Stomach Meridian group, gallbladder Meridian group, Stomach Meridian plus PD153035 group and gallbladder Meridian plus PD153035 group. Water-immersion and restrained stress methods were adopted for inducing gastric mucosal injury in the rats. Gastric mucosal cells were separated by using pronase digestion method, and incubated by PD153035, a EGFR inhibitor, and 100 ml/L serum. The expression of PLC gamma-1 in the gastric mucosal cells was tested by enzyme linked-immunosorbent assay (ELISA), while the expression of PKC by isotope incorporate assay and the expression of c-myc by reverse transcription polymerase chain reaction assay (RT-PCR). RESULTS: In gastric mucosal cells, weak expressions of PLC gamma-1, PKC and c-myc were seen in the normal group, and relatively strong expressions of PLC gamma-1, PKC and c-myc were seen in the Stomach Meridian group and the gallbladder Meridian group, among which, the expressions of PLC gamma-1, PKC and c-myc in the Stomach Meridian group were the strongest, and there was a significant difference between the Stomach Meridian group and the gallbladder Meridian group (P<0.01). Relative weak expressions of PLC gamma-1, PKC and c-myc were seen in the Stomach Meridian plus PD153035 group and the gallbladder Meridian plus PD153035 group, and there was a significant difference between the Stomach Meridian group and the Stomach Meridian plus PD153035 group (P<0.01). CONCLUSIONS: The serum derived from the rats treated with electroacupuncture at Stomach Meridian acupoints can activate the EGFR singling pathway, and this provides an evidence for the theory of "relative particularity between Meridians and viscera" in traditional Chinese medicine.
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enhanced expression of epidermal growth factor receptor gene in gastric mucosal cells by the serum derived from rats treated with electroacupuncture at Stomach Meridian acupoints
World Journal of Gastroenterology, 2006Co-Authors: Zong-bao Yang, Jie Yan, Xiao-rong Chang, Xiaoping Zou, Yaping LinAbstract:Enhanced expression of epidermal growth factor receptor gene in gastric mucosal cells by the serum derived from rats treated with electroacupuncture at Stomach Meridian acupoints
Walter N Duran - One of the best experts on this subject based on the ideXlab platform.
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acupuncture reduces experimental renovascular hypertension through mechanisms involving nitric oxide synthases
Microcirculation, 2006Co-Authors: David D. Kim, Arnaldo Michel Pica, Ricardo G Duran, Walter N DuranAbstract:Objective: To test the hypothesis that acupuncture on Stomach 36 point (ST-36) reduces hypertension by activating nitric oxide synthase signaling mechanisms. Methods: The authors used the two-kidney, one-clip renal hypertension (2K1C) hamster model with electroacupuncture treatment. Results: Thirty-minute daily electroacupuncture treatment for 5 days reduced mean arterial pressure from 160.0 ± 7.6 to 128.0 ± 4.3 mmHg (mean ± SEM), compared to 115.0 ± 7.2 mmHg in sham-operated hamsters. Electroacupuncture increased periarteriolar NO concentration from 309.0 ± 21.7 nM to 417.9 ± 20.9 nM in the 2K1C hamster cheek pouch microcirculation when measured with NO-sensitive microelectrodes. Hypertension reduced endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) proteins relative to the sham-operated control, as measured by Western blotting. Electroacupuncture prevented the reduction of eNOS and nNOS associated with hypertension and showed even higher eNOS and nNOS expressions than sham-operated control in Stomach and cheek pouch tissues, which are on the Stomach Meridian. Analysis of liver tissue, a nonStomach-Meridian organ, indicated that electroacupuncture did not have a significant benefit in terms of enhanced expressions of eNOS and nNOS in the treated 2K1C hypertensive group.
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electroacupuncture on Stomach 36 point st 36 reduces hypertension through endothelial nitric oxide synthase enos activity in the Stomach Meridian
The FASEB Journal, 2006Co-Authors: Arnaldo Michel Pica, Ricardo G Duran, Walter N Duran, David D. KimAbstract:Acupuncture has been used in Asia to treat hypertension for a long time. We hypothesized that acupuncture on ST-36 activates eNOS signaling mechanisms to reduce hypertension. We tested the hypothes...
Arnaldo Michel Pica - One of the best experts on this subject based on the ideXlab platform.
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acupuncture reduces experimental renovascular hypertension through mechanisms involving nitric oxide synthases
Microcirculation, 2006Co-Authors: David D. Kim, Arnaldo Michel Pica, Ricardo G Duran, Walter N DuranAbstract:Objective: To test the hypothesis that acupuncture on Stomach 36 point (ST-36) reduces hypertension by activating nitric oxide synthase signaling mechanisms. Methods: The authors used the two-kidney, one-clip renal hypertension (2K1C) hamster model with electroacupuncture treatment. Results: Thirty-minute daily electroacupuncture treatment for 5 days reduced mean arterial pressure from 160.0 ± 7.6 to 128.0 ± 4.3 mmHg (mean ± SEM), compared to 115.0 ± 7.2 mmHg in sham-operated hamsters. Electroacupuncture increased periarteriolar NO concentration from 309.0 ± 21.7 nM to 417.9 ± 20.9 nM in the 2K1C hamster cheek pouch microcirculation when measured with NO-sensitive microelectrodes. Hypertension reduced endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) proteins relative to the sham-operated control, as measured by Western blotting. Electroacupuncture prevented the reduction of eNOS and nNOS associated with hypertension and showed even higher eNOS and nNOS expressions than sham-operated control in Stomach and cheek pouch tissues, which are on the Stomach Meridian. Analysis of liver tissue, a nonStomach-Meridian organ, indicated that electroacupuncture did not have a significant benefit in terms of enhanced expressions of eNOS and nNOS in the treated 2K1C hypertensive group.
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electroacupuncture on Stomach 36 point st 36 reduces hypertension through endothelial nitric oxide synthase enos activity in the Stomach Meridian
The FASEB Journal, 2006Co-Authors: Arnaldo Michel Pica, Ricardo G Duran, Walter N Duran, David D. KimAbstract:Acupuncture has been used in Asia to treat hypertension for a long time. We hypothesized that acupuncture on ST-36 activates eNOS signaling mechanisms to reduce hypertension. We tested the hypothes...