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Nandagopal Bakthavatsalam - One of the best experts on this subject based on the ideXlab platform.
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Leaves of Lantana camara Linn. (Verbenaceae) as a potential insecticide for the management of three species of Stored Grain insect pests
Journal of Food Science and Technology, 2014Co-Authors: Yallappa Rajashekar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Insects cause extensive damage to Stored Grains and their value added products. Among the Stored Grain pests Sitophilus oryzae (L.) Callosobruchus chinensis (Fab.) and Tribolium castaneum (Herbst.) are considered as destructive pests in India. Plants may provide alternatives to currently used insect control agents as they constitute rich source in bioactive molecules. Lantana camara, an erect shrub, which grows widely in the tropics, exhibits insecticidal activity against several insects. The methanol extract from leaves of L. camara has fumigant and contact toxicity against S. oryzae, C. chinesis and T. castaneum . In fumigant assays, The LC_50 for S. oryzae was 128 μl/L^1, C. chinensis 130.3 μl/L^1, and T. castaneum 178.7 μl/L^1. The LD_50 values for S. oryzae C. chinensis and T. castaneum in contact toxicity were 0.158, 0.140 and 0.208 mg/cm^2, respectively. For Grain treatment, a concentration of 500 mg/L^1 and 7 days exposure were needed to obtain 90 – 100 % population extinction in all three insects. Probit analysis showed that C. chinensis were more susceptible than S. oryzae and T. castaneum. Gaschromatography-Mass Spectrometer (GCMS) studies for extracts indicated the presence of potent fumigant molecules in L. camara . The prospect of utilizing L. camara as potent fumigant insecticide is discussed.
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leaves of lantana camara linn verbenaceae as a potential insecticide for the management of three species of Stored Grain insect pests
Journal of Food Science and Technology-mysore, 2014Co-Authors: Yallappa Rajashekar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Insects cause extensive damage to Stored Grains and their value added products. Among the Stored Grain pests Sitophilus oryzae (L.) Callosobruchus chinensis (Fab.) and Tribolium castaneum (Herbst.) are considered as destructive pests in India. Plants may provide alternatives to currently used insect control agents as they constitute rich source in bioactive molecules. Lantana camara, an erect shrub, which grows widely in the tropics, exhibits insecticidal activity against several insects. The methanol extract from leaves of L. camara has fumigant and contact toxicity against S. oryzae, C. chinesis and T. castaneum. In fumigant assays, The LC50 for S. oryzae was 128 μl/L1, C. chinensis 130.3 μl/L1, and T. castaneum 178.7 μl/L1. The LD50 values for S. oryzae C. chinensis and T. castaneum in contact toxicity were 0.158, 0.140 and 0.208 mg/cm2, respectively. For Grain treatment, a concentration of 500 mg/L1 and 7 days exposure were needed to obtain 90 – 100 % population extinction in all three insects. Probit analysis showed that C. chinensis were more susceptible than S. oryzae and T. castaneum. Gaschromatography-Mass Spectrometer (GCMS) studies for extracts indicated the presence of potent fumigant molecules in L. camara. The prospect of utilizing L. camara as potent fumigant insecticide is discussed. Electronic supplementary material The online version of this article (doi:10.1007/s13197-012-0884-8) contains supplementary material, which is available to authorized users.
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acetylcholinesterase inhibition by biofumigant coumaran from leaves of lantana camara in Stored Grain and household insect pests
BioMed Research International, 2014Co-Authors: Yallappa Rajashekar, Anjanappa Raghavendra, Nandagopal BakthavatsalamAbstract:Recent studies proved that the biofumigants could be an alternative to chemical fumigants against Stored Grain insect pests. For this reason, it is necessary to understand the mode of action of biofumigants. In the present study the prospectus of utilising Lantana camara as a potent fumigant insecticide is being discussed. Inhibition of acetylcholinesterase (AChE) by Coumaran, an active ingredient extracted from the plant L. camara, was studied. The biofumigant was used as an enzyme inhibitor and acetylthiocholine iodide as a substrate along with Ellman's reagent to carry out the reactions. The in vivo inhibition was observed in both dose dependent and time dependent in case of housefly, and the nervous tissue (ganglion) and the whole insect homogenate of Stored Grain insect exposed to Coumaran. The possible mode of action of Coumaran as an acetylcholinesterase inhibitor is discussed.
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isolation and characterization of biofumigant from leaves of lantana camara for control of Stored Grain insect pests
Industrial Crops and Products, 2013Co-Authors: Yallappa Rajashekar, Honnaiah Vijay Kumar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Due to environmental concerns, health hazards to man and the evolution of resistance in insect pests, there have been constant efforts to discover newer insecticides both from natural sources and by chemical synthesis. Natural sources for novel molecules hold promise in view of their eco-friendly nature, selectivity and mammalian safety. We have isolated one natural bioactive molecule from the leaves of Lantana camara named Coumaran, based on various physical-chemical and spectroscopic techniques (IR, H-1 NMR, C-13 NMR and MS). Coumaran is highly toxic and very low concentration is needed for control of Stored product insects. This molecule has potent Grain protectant potential and caused significant reduction in F1 progeny of all the three species in the treated Grain and the progeny was completely suppressed at 30 mu g/l. The differences in germination between the control and treated Grains were not significant. The lack of any adverse effect of Coumaran on the seed germination is highly desirable for a Grain protectant, becoming a potential source of biofumigant for economical and environmentally friendly pest control strategies against Stored Grain pests during storage of Grains or pulses. (C) 2013 Elsevier B.V. All rights reserved.
Yallappa Rajashekar - One of the best experts on this subject based on the ideXlab platform.
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Leaves of Lantana camara Linn. (Verbenaceae) as a potential insecticide for the management of three species of Stored Grain insect pests
Journal of Food Science and Technology, 2014Co-Authors: Yallappa Rajashekar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Insects cause extensive damage to Stored Grains and their value added products. Among the Stored Grain pests Sitophilus oryzae (L.) Callosobruchus chinensis (Fab.) and Tribolium castaneum (Herbst.) are considered as destructive pests in India. Plants may provide alternatives to currently used insect control agents as they constitute rich source in bioactive molecules. Lantana camara, an erect shrub, which grows widely in the tropics, exhibits insecticidal activity against several insects. The methanol extract from leaves of L. camara has fumigant and contact toxicity against S. oryzae, C. chinesis and T. castaneum . In fumigant assays, The LC_50 for S. oryzae was 128 μl/L^1, C. chinensis 130.3 μl/L^1, and T. castaneum 178.7 μl/L^1. The LD_50 values for S. oryzae C. chinensis and T. castaneum in contact toxicity were 0.158, 0.140 and 0.208 mg/cm^2, respectively. For Grain treatment, a concentration of 500 mg/L^1 and 7 days exposure were needed to obtain 90 – 100 % population extinction in all three insects. Probit analysis showed that C. chinensis were more susceptible than S. oryzae and T. castaneum. Gaschromatography-Mass Spectrometer (GCMS) studies for extracts indicated the presence of potent fumigant molecules in L. camara . The prospect of utilizing L. camara as potent fumigant insecticide is discussed.
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leaves of lantana camara linn verbenaceae as a potential insecticide for the management of three species of Stored Grain insect pests
Journal of Food Science and Technology-mysore, 2014Co-Authors: Yallappa Rajashekar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Insects cause extensive damage to Stored Grains and their value added products. Among the Stored Grain pests Sitophilus oryzae (L.) Callosobruchus chinensis (Fab.) and Tribolium castaneum (Herbst.) are considered as destructive pests in India. Plants may provide alternatives to currently used insect control agents as they constitute rich source in bioactive molecules. Lantana camara, an erect shrub, which grows widely in the tropics, exhibits insecticidal activity against several insects. The methanol extract from leaves of L. camara has fumigant and contact toxicity against S. oryzae, C. chinesis and T. castaneum. In fumigant assays, The LC50 for S. oryzae was 128 μl/L1, C. chinensis 130.3 μl/L1, and T. castaneum 178.7 μl/L1. The LD50 values for S. oryzae C. chinensis and T. castaneum in contact toxicity were 0.158, 0.140 and 0.208 mg/cm2, respectively. For Grain treatment, a concentration of 500 mg/L1 and 7 days exposure were needed to obtain 90 – 100 % population extinction in all three insects. Probit analysis showed that C. chinensis were more susceptible than S. oryzae and T. castaneum. Gaschromatography-Mass Spectrometer (GCMS) studies for extracts indicated the presence of potent fumigant molecules in L. camara. The prospect of utilizing L. camara as potent fumigant insecticide is discussed. Electronic supplementary material The online version of this article (doi:10.1007/s13197-012-0884-8) contains supplementary material, which is available to authorized users.
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acetylcholinesterase inhibition by biofumigant coumaran from leaves of lantana camara in Stored Grain and household insect pests
BioMed Research International, 2014Co-Authors: Yallappa Rajashekar, Anjanappa Raghavendra, Nandagopal BakthavatsalamAbstract:Recent studies proved that the biofumigants could be an alternative to chemical fumigants against Stored Grain insect pests. For this reason, it is necessary to understand the mode of action of biofumigants. In the present study the prospectus of utilising Lantana camara as a potent fumigant insecticide is being discussed. Inhibition of acetylcholinesterase (AChE) by Coumaran, an active ingredient extracted from the plant L. camara, was studied. The biofumigant was used as an enzyme inhibitor and acetylthiocholine iodide as a substrate along with Ellman's reagent to carry out the reactions. The in vivo inhibition was observed in both dose dependent and time dependent in case of housefly, and the nervous tissue (ganglion) and the whole insect homogenate of Stored Grain insect exposed to Coumaran. The possible mode of action of Coumaran as an acetylcholinesterase inhibitor is discussed.
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isolation and characterization of biofumigant from leaves of lantana camara for control of Stored Grain insect pests
Industrial Crops and Products, 2013Co-Authors: Yallappa Rajashekar, Honnaiah Vijay Kumar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Due to environmental concerns, health hazards to man and the evolution of resistance in insect pests, there have been constant efforts to discover newer insecticides both from natural sources and by chemical synthesis. Natural sources for novel molecules hold promise in view of their eco-friendly nature, selectivity and mammalian safety. We have isolated one natural bioactive molecule from the leaves of Lantana camara named Coumaran, based on various physical-chemical and spectroscopic techniques (IR, H-1 NMR, C-13 NMR and MS). Coumaran is highly toxic and very low concentration is needed for control of Stored product insects. This molecule has potent Grain protectant potential and caused significant reduction in F1 progeny of all the three species in the treated Grain and the progeny was completely suppressed at 30 mu g/l. The differences in germination between the control and treated Grains were not significant. The lack of any adverse effect of Coumaran on the seed germination is highly desirable for a Grain protectant, becoming a potential source of biofumigant for economical and environmentally friendly pest control strategies against Stored Grain pests during storage of Grains or pulses. (C) 2013 Elsevier B.V. All rights reserved.
Gregory J Daglish - One of the best experts on this subject based on the ideXlab platform.
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testing the consistency of spatio temporal patterns of flight activity in the Stored Grain beetles tribolium castaneum herbst and rhyzopertha dominica f
Journal of Stored Products Research, 2017Co-Authors: Gregory J Daglish, A W Ridley, Robin Reid, G H WalterAbstract:Many studies have been published on the flight activity of Stored Grain beetles in temperate regions, but few have focussed on tropical and sub-tropical regions. The spatio-temporal patterns of flight activity of Tribolium castaneum (Herbst) and Rhyzopertha dominica (F.) were quantified in a Grain growing district on the Tropic of Capricorn in central Queensland, Australia. Nine traps baited with aggregation pheromone lures were monitored at 2-wk intervals (fortnightly) for 1 year along a 28.4 km linear transect that included sites at bulk Grain depots and sites away from Stored Grain. Beetles of both species were trapped every fortnight during the study. The spatio-temporal patterns of flight activity differed greatly across the two species, as predicted from studies elsewhere. Rhyzopertha dominica was widespread across the landscape, as the mean trap catch of this species was equal in depot and non-depot traps. In contrast, T. castaneum was more frequently trapped in depot traps than non-depot traps during the colder months, but was much more widespread across the landscape during the summer months. Tribolium castaneum also showed a clear mid-summer peak in flight activity, whereas R. dominica flight activity was highly variable throughout the study. In general, our results reveal patterns that are consistent with those found for T. castaneum and R. dominica in southern Queensland. The contrasting spatio-temporal patterns of flight activity of R. dominica and T. castaneum show that species-specific approaches may be needed to manage these pests and that the spatio-temporal dynamics of resistance genes may differ across these species.
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implications of methoprene resistance for managing rhyzopertha dominica f in Stored Grain
Journal of Stored Products Research, 2013Co-Authors: Gregory J Daglish, Joanne C Holloway, Manoj K NayakAbstract:Commercial formulations of methoprene have been used in a number of countries such as Australia and the USA to provide long-term protection to Grain from a range of storage pests. The level of resistance in Rhyzopertha dominica (F.), the lesser Grain borer, was investigated in laboratory experiments by direct exposure of adults on treated wheat. Adults of a reference homozygous resistant strain of R.dominica were exposed to treatments of 0, 2, 3, 5, 10, 20 and 40kg-1 of s-methoprene for 7 days. Mortality and progeny production were dose dependent with 98.7% mortality and complete progeny suppression at 40mgkg-1, which is 67 times the registered rate at which s-methoprene is applied as a Grain protectant in Australia (0.6mgkg-1) and eight times the rate which has been used in the USA (5mgkg-1). This strain was also tested by adding adults to wheat treated at 0, 1, 3, 10 and 30mgkg-1 and determining the number of adults (progeny plus original parental adults) after 6, 8, 10 or 12 weeks of continuous exposure. The effect of s-methoprene was consistent regardless of the number of weeks of continuous exposure and average population suppression was 99.5% at 30mgkg-1. Screening of 162 field samples collected from southeast Australia in 2009 showed that 93% of samples reproduced when exposed to wheat treated with the Australian registered rate of 0.6mgkg-1. When four unselected resistant field samples from this region were tested, progeny production but not mortality was dose dependent, and all four samples produced live progeny at the highest dose of 30mgkg-1. Our results show that methoprene resistance is a serious threat to the management of R.dominica, and that strategies need to be developed to minimise the further development and spread of resistance. © 2013.
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impact of resistance on the efficacy of binary combinations of spinosad chlorpyrifos methyl and s methoprene against five Stored Grain beetles
Journal of Stored Products Research, 2008Co-Authors: Gregory J DaglishAbstract:Laboratory experiments were conducted to determine the efficacy of spinosad (a biopesticide), chlorpyrifos-methyl (an organophosphorus compound (OP)) and s-methoprene (a juvenile hormone analogue) applied alone and in binary combinations against five Stored-Grain beetles in wheat. There were three strains of Rhyzopertha dominica, and one strain each of Sitophilus oryzae, Tribolium castaneum, Oryzaephilus surinamensis and Cryptolestes ferrugineus. These strains were chosen to represent a range of possible resistant genotypes, exhibiting resistance to organophosphates, pyrethroids or methoprene. Treatments were applied at rates that are registered or likely to be registered in Australia. Adults were exposed to freshly treated wheat for 2 weeks, and the effects of treatments on mortality and reproduction were determined. No single protectant or protectant combination controlled all insect strains, based on the criterion of >99% reduction in the number of live F1 adults relative to the control. The most effective combinations were spinosad at 1 mg kg-1+chlorpyrifos-methyl at 10 mg kg-1 which controlled all strains except for OP-resistant O. surinamensis, and chlorpyrifos-methyl at 10 mg kg-1+s-methoprene at 0.6 mg kg-1 which controlled all strains except for methoprene-resistant R. dominica. The results of this study demonstrate the difficulty in Australia, and potentially other countries which use protectants, of finding protectant treatments to control a broad range of pest species in the face of resistance development.
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combined treatments of spinosad and chlorpyrifos methyl for management of resistant psocid pests psocoptera liposcelididae of Stored Grain
Pest Management Science, 2007Co-Authors: Manoj K Nayak, Gregory J DaglishAbstract:The combined efficacy of spinosad and chlorpyrifos-methyl was determined against four storage psocid pests belonging to genus Liposcelis. This research was undertaken because of the increasing importance of these psocids in Stored Grain and the problem of finding Grain protectants to control resistant strains. Firstly, mortality and reproduction were determined for adults exposed to wheat freshly treated with either spinosad (0.5 and 1 mg kg-1) or chlorpyrifos-methyl (2.5, 5 and 10 mg kg-1) or combinations of spinosad and chlorpyrifos-methyl at 30°C and 70% RH. There were significant effects of application rate of spinosad and chlorpyrifos-methyl, both individually and in combination, on adult mortality and progeny reduction of all four psocids. Liposcelis bostrychophila Badonnel and L. decolor (Pearman) responded similarly, with incomplete control of adults and progeny at both doses of spinosad but complete control in all chlorpyrifos-methyl and combined treatments. In L. entomophila (Enderlein) and L. paeta Pearman, however, complete control of adults and progeny was only achieved in the combined treatments, with the exception of spinosad 0.5 mg kg-1 plus chlorpyrifos-methyl 2.5 mg kg-1 against L. entomophila. Next, combinations of spinosad (0.5 and 1 mg kg-1) and chlorpyrifos-methyl (2.5, 5 and 10 mg kg-1) in bioassays after 0, 1.5 and 3 months storage of treated wheat were evaluated. The best treatment was 1 mg kg -1 of spinosad plus 10 mg kg-1 of chlorpyrifos-methyl, providing up to 3 months of protection against infestations of all four Liposcelis spp. on wheat.
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potential of imidacloprid to control four species of psocids psocoptera liposcelididae infesting Stored Grain
Pest Management Science, 2006Co-Authors: Manoj K Nayak, Gregory J DaglishAbstract:The effectiveness of the neonicotinoid insecticide imidacloprid was evaluated against four psocid pests of Stored Grain. This research was undertaken because of the growing importance of psocids in Stored Grain and the need to identify methods for their control. The mortality and reproduction of adults of Liposcelis bostrychophila Badonnel, L. entomophila (Enderlein), L. decolor (Pearman) and L. paeta Pearman in wheat treated with imidacloprid were determined. There were five application rates (0.5, 1, 2, 5 and 10 mg AI kg -1 Grain) and an untreated control. There were significant effects of application rate on both adult mortality and reproduction for all four species, but the effect of imidacloprid was sometimes more pronounced on reproduction. Imidacloprid was most effective against L. bostrychophila, with 100% adult mortality after 7 d at 5 mg AI kg-1, 14 d at 2 mg AI kg-1 and 28 d at 0.5 and 1 mg AI kg-1. No live progeny were produced at 2 mg AI kg-1. For L. decolor, there was 100% adult mortality after 28 d at 10 mg AI kg-1 and no live progeny were produced at 2 mg AI kg-1. For L. entomophila, there was 100% adult mortality after 14 d at 10 mg AI kg-1 and 28 d at 2 and 5 mg AI kg-1. No live progeny were produced at 10 mg AI kg-1. At 10 mg AI kg-1 there was 100% mortality of L. paeta adults after 28 d exposure and no live progeny developed. Because reproduction at some application rates occurred only in the first 14 d of exposure, it is concluded that the application rate leading to population extinction was 1 mg AI kg-1 for L. bostrychophila, 2 mg AI kg-1 for L. decolor and L. entomophila and 5 mg AI kg -1 for L. paeta. This study shows that imidacloprid has potential as a Grain protectant to control all four Liposcelis species in Stored Grain.
Martin Kaltenpoth - One of the best experts on this subject based on the ideXlab platform.
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Ancient symbiosis confers desiccation resistance to Stored Grain pest beetles
Molecular Ecology, 2017Co-Authors: Tobias Engl, Nadia Eberl, Theresa Krüger, Thorsten H. P. Schmidt, Rudy Plarre, Cornel Adler, Carla Gorse, Martin KaltenpothAbstract:Microbial symbionts of insects provide a range of ecological traits to their hosts that are beneficial in the context of biotic interactions. However, little is known about insect symbiont-mediated adaptation to the abiotic environment, for example, temperature and humidity. Here, we report on an ancient clade of intracellular, bacteriome-located Bacteroidetes symbionts that are associated with Grain and Wood pest beetles of the phylogenetically distant families Silvanidae and Bostrichidae. In the saw-toothed Grain beetle Oryzaephilus surinamensis, we demonstrate that the symbionts affect cuticle thickness, melanization and hydrocarbon profile, enhancing desiccation resistance and thereby strongly improving fitness under dry conditions. Together with earlier observations on Symbiont contributions to cuticle biosynthesis in weevils, our findings indicate that convergent acquisitions of bacterial mutualists represented key adaptations enabling diverse pest beetle groups to survive and proliferate under the low ambient humidity that characterizes dry Grain storage facilities.
Kothapalli V Ravindra - One of the best experts on this subject based on the ideXlab platform.
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leaves of lantana camara linn verbenaceae as a potential insecticide for the management of three species of Stored Grain insect pests
Journal of Food Science and Technology-mysore, 2014Co-Authors: Yallappa Rajashekar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Insects cause extensive damage to Stored Grains and their value added products. Among the Stored Grain pests Sitophilus oryzae (L.) Callosobruchus chinensis (Fab.) and Tribolium castaneum (Herbst.) are considered as destructive pests in India. Plants may provide alternatives to currently used insect control agents as they constitute rich source in bioactive molecules. Lantana camara, an erect shrub, which grows widely in the tropics, exhibits insecticidal activity against several insects. The methanol extract from leaves of L. camara has fumigant and contact toxicity against S. oryzae, C. chinesis and T. castaneum. In fumigant assays, The LC50 for S. oryzae was 128 μl/L1, C. chinensis 130.3 μl/L1, and T. castaneum 178.7 μl/L1. The LD50 values for S. oryzae C. chinensis and T. castaneum in contact toxicity were 0.158, 0.140 and 0.208 mg/cm2, respectively. For Grain treatment, a concentration of 500 mg/L1 and 7 days exposure were needed to obtain 90 – 100 % population extinction in all three insects. Probit analysis showed that C. chinensis were more susceptible than S. oryzae and T. castaneum. Gaschromatography-Mass Spectrometer (GCMS) studies for extracts indicated the presence of potent fumigant molecules in L. camara. The prospect of utilizing L. camara as potent fumigant insecticide is discussed. Electronic supplementary material The online version of this article (doi:10.1007/s13197-012-0884-8) contains supplementary material, which is available to authorized users.
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Leaves of Lantana camara Linn. (Verbenaceae) as a potential insecticide for the management of three species of Stored Grain insect pests
Journal of Food Science and Technology, 2014Co-Authors: Yallappa Rajashekar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Insects cause extensive damage to Stored Grains and their value added products. Among the Stored Grain pests Sitophilus oryzae (L.) Callosobruchus chinensis (Fab.) and Tribolium castaneum (Herbst.) are considered as destructive pests in India. Plants may provide alternatives to currently used insect control agents as they constitute rich source in bioactive molecules. Lantana camara, an erect shrub, which grows widely in the tropics, exhibits insecticidal activity against several insects. The methanol extract from leaves of L. camara has fumigant and contact toxicity against S. oryzae, C. chinesis and T. castaneum . In fumigant assays, The LC_50 for S. oryzae was 128 μl/L^1, C. chinensis 130.3 μl/L^1, and T. castaneum 178.7 μl/L^1. The LD_50 values for S. oryzae C. chinensis and T. castaneum in contact toxicity were 0.158, 0.140 and 0.208 mg/cm^2, respectively. For Grain treatment, a concentration of 500 mg/L^1 and 7 days exposure were needed to obtain 90 – 100 % population extinction in all three insects. Probit analysis showed that C. chinensis were more susceptible than S. oryzae and T. castaneum. Gaschromatography-Mass Spectrometer (GCMS) studies for extracts indicated the presence of potent fumigant molecules in L. camara . The prospect of utilizing L. camara as potent fumigant insecticide is discussed.
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isolation and characterization of biofumigant from leaves of lantana camara for control of Stored Grain insect pests
Industrial Crops and Products, 2013Co-Authors: Yallappa Rajashekar, Honnaiah Vijay Kumar, Kothapalli V Ravindra, Nandagopal BakthavatsalamAbstract:Due to environmental concerns, health hazards to man and the evolution of resistance in insect pests, there have been constant efforts to discover newer insecticides both from natural sources and by chemical synthesis. Natural sources for novel molecules hold promise in view of their eco-friendly nature, selectivity and mammalian safety. We have isolated one natural bioactive molecule from the leaves of Lantana camara named Coumaran, based on various physical-chemical and spectroscopic techniques (IR, H-1 NMR, C-13 NMR and MS). Coumaran is highly toxic and very low concentration is needed for control of Stored product insects. This molecule has potent Grain protectant potential and caused significant reduction in F1 progeny of all the three species in the treated Grain and the progeny was completely suppressed at 30 mu g/l. The differences in germination between the control and treated Grains were not significant. The lack of any adverse effect of Coumaran on the seed germination is highly desirable for a Grain protectant, becoming a potential source of biofumigant for economical and environmentally friendly pest control strategies against Stored Grain pests during storage of Grains or pulses. (C) 2013 Elsevier B.V. All rights reserved.
