The Experts below are selected from a list of 924 Experts worldwide ranked by ideXlab platform
Maria Immacolata Ferrante - One of the best experts on this subject based on the ideXlab platform.
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Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta
BMC genomics, 2015Co-Authors: Shrikant Patil, Sara Moeys, Peter Von Dassow, Marie J. J. Huysman, Daniel Mapleson, Lieven De Veylder, Remo Sanges, Wim Vyverman, Marina Montresor, Maria Immacolata FerranteAbstract:Sexual reproduction is an obligate phase in the life cycle of most eukaryotes. Meiosis varies among organisms, which is reflected by the variability of the gene set associated to the process. Diatoms are unicellular organisms that belong to the Stramenopile clade and have unique life cycles that can include a sexual phase. The exploration of five diatom genomes and one diatom transcriptome led to the identification of 42 genes potentially involved in meiosis. While these include the majority of known meiosis-related genes, several meiosis-specific genes, including DMC1, could not be identified. Furthermore, phylogenetic analyses supported gene identification and revealed ancestral loss and recent expansion in the RAD51 family in diatoms. The two sexual species Pseudo-nitzschia multistriata and Seminavis robusta were used to explore the expression of meiosis-related genes: RAD21, SPO11-2, RAD51-A, RAD51-B and RAD51-C were upregulated during meiosis, whereas other paralogs in these families showed no differential expression patterns, suggesting that they may play a role during vegetative divisions. An almost identical toolkit is shared among Pseudo-nitzschia multiseries and Fragilariopsis cylindrus, as well as two species for which sex has not been observed, Phaeodactylum tricornutum and Thalassiosira pseudonana, suggesting that these two may retain a facultative sexual phase. Our results reveal the conserved meiotic toolkit in six diatom species and indicate that Stramenopiles share major modifications of canonical meiosis processes ancestral to eukaryotes, with important divergences in each Kingdom.
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Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta
BMC Genomics, 2015Co-Authors: Shrikant Patil, Sara Moeys, Peter Von Dassow, Marie J. J. Huysman, Daniel Mapleson, Lieven De Veylder, Remo Sanges, Wim Vyverman, Marina Montresor, Maria Immacolata FerranteAbstract:Background Sexual reproduction is an obligate phase in the life cycle of most eukaryotes. Meiosis varies among organisms, which is reflected by the variability of the gene set associated to the process. Diatoms are unicellular organisms that belong to the Stramenopile clade and have unique life cycles that can include a sexual phase. Results The exploration of five diatom genomes and one diatom transcriptome led to the identification of 42 genes potentially involved in meiosis. While these include the majority of known meiosis-related genes, several meiosis-specific genes, including DMC1, could not be identified. Furthermore, phylogenetic analyses supported gene identification and revealed ancestral loss and recent expansion in the RAD51 family in diatoms. The two sexual species Pseudo-nitzschia multistriata and Seminavis robusta were used to explore the expression of meiosis-related genes: RAD21, SPO11-2, RAD51-A, RAD51-B and RAD51-C were upregulated during meiosis, whereas other paralogs in these families showed no differential expression patterns, suggesting that they may play a role during vegetative divisions. An almost identical toolkit is shared among Pseudo-nitzschia multiseries and Fragilariopsis cylindrus, as well as two species for which sex has not been observed, Phaeodactylum tricornutum and Thalassiosira pseudonana, suggesting that these two may retain a facultative sexual phase. Conclusions Our results reveal the conserved meiotic toolkit in six diatom species and indicate that Stramenopiles share major modifications of canonical meiosis processes ancestral to eukaryotes, with important divergences in each Kingdom.
William G T Willats - One of the best experts on this subject based on the ideXlab platform.
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insoluble 1 3 1 4 β d glucan is a component of cell walls in brown algae phaeophyceae and is masked by alginates in tissues
Scientific Reports, 2017Co-Authors: Armando A Salmean, Delphine Duffieux, Jesper Harholt, Gurvan Michel, Mirjam Czjzek, William G T WillatsAbstract:Brown algae are photosynthetic multicellular marine organisms. They belong to the phylum of Stramenopiles, which are not closely related to land plants and green algae. Brown algae share common evolutionary features with other photosynthetic and multicellular organisms, including a carbohydrate-rich cell-wall. Brown algal cell walls are composed predominantly of the polyanionic polysaccharides alginates and fucose-containing sulfated polysaccharides. These polymers are prevalent over neutral and crystalline components, which are believed to be mostly, if not exclusively, cellulose. In an attempt to better understand brown algal cell walls, we performed an extensive glycan array analysis of a wide range of brown algal species. Here we provide the first demonstration that mixed-linkage (1 → 3), (1 → 4)-β-d-glucan (MLG) is common in brown algal cell walls. Ultra-Performance Liquid Chromatography analyses indicate that MLG in brown algae solely consists of trisaccharide units of contiguous (1 → 4)-β-linked glucose residues joined by (1 → 3)-β-linkages. This regular conformation may allow long stretches of the molecule to align and to form well-structured microfibrils. At the tissue level, immunofluorescence studies indicate that MLG epitopes in brown algae are unmasked by a pre-treatment with alginate lyases to remove alginates. These findings are further discussed in terms of the origin and evolution of MLG in the Stramenopile lineage.
Adrienne R Hardham - One of the best experts on this subject based on the ideXlab platform.
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Characterisation of Stramenopile-specific mastigoneme proteins in Phytophthora parasitica
Protoplasma, 2019Co-Authors: Leila M. Blackman, Adrienne R HardhamAbstract:Mastigonemes, tripartite tubular hairs on the anterior flagellum of Phytophthora zoospores, are instrumental for disease dissemination to new host plants. A previous study showed that PnMas2 was part of the tubular shaft of Phytophthora parasitica mastigonemes. In the current study, genes encoding two related proteins, PnMas1 and PnMas3, were identified in the genome of P. parasitica . PnMas1 interacts with PnMas2 and also occurs along the mastigoneme shaft. RNA-Seq analyses indicate that PnMas1 and PnMas2 genes have similar expression profiles both in vitro and in planta but that PnMas3 is expressed temporally prior to PnMas1 and PnMas2 during asexual development and plant infection. Immunocytochemistry and GFP-tagging document the occurrence of all three PnMas proteins within the specialised compartments of the ER during mastigoneme formation, but only PnMas1 and PnMas2 occur in mature mastigonemes on the flagellar surface. Anti-PnMas1 and anti-PnMas2 antibodies co-labelled two high-molecular-weight (~400 kDa) protein complexes in native gels but anti-PnMas3 antibodies labelled a 65 kDa protein complex. Liquid chromatography-mass spectrometry analysis identified PnMas1 and PnMas2 but not PnMas3 in flagellar extracts. These results suggest that PnMas3 associates with mastigonemes during their assembly within the ER but is not part of mature mastigonemes on the anterior flagellum. Phylogenetic analyses using homologues of Mas genes from the genomes of 28 species of Stramenopiles give evidence of three Mas sub-families, namely Mas1, Mas2 and Mas3. BLAST analyses showed that Mas genes only occur in flagellate species within the Stramenopile taxon.
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proteins related to green algal striated fiber assemblin are present in Stramenopiles and alveolates
Protoplasma, 2009Co-Authors: John D I Harper, Jacques Thuet, Karlferdinand Lechtreck, Adrienne R HardhamAbstract:In green algae, striated fiber assemblin (SFA) is the major protein of the striated microtubule-associated fibers that are structural elements in the flagellar basal apparatus. Using Basic Local Alignment Search Tool (BLAST) searches of recently established databases, SFA-like sequences were detected in the genomes not only of green algal species but also of a range of other protists. These included species in two alveolate subgroups, the ciliates (Tetrahymena thermophila, Paramecium tetraurelia) and the dinoflagellates (Perkinsus marinus), and two Stramenopile subgroups, the oomycetes (Phytophthora sojae, Phytophthora ramorum, Phytophthora infestans) and the diatoms (Thalassiosira pseudonana, Phaeodactylum tricornutum). Together with earlier identification of SFA-like sequences in the apicomplexans, these results indicate that homologs of SFA are present across the alveolates and Stramenopiles. Antibodies raised against SFA from the green alga, Spermatozopsis similis, react in immunofluorescence assays with the two basal bodies and an anteriorly directed striated fiber in the flagellar apparatus of biflagellate Phytophthora zoospores.
Shrikant Patil - One of the best experts on this subject based on the ideXlab platform.
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Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta
BMC genomics, 2015Co-Authors: Shrikant Patil, Sara Moeys, Peter Von Dassow, Marie J. J. Huysman, Daniel Mapleson, Lieven De Veylder, Remo Sanges, Wim Vyverman, Marina Montresor, Maria Immacolata FerranteAbstract:Sexual reproduction is an obligate phase in the life cycle of most eukaryotes. Meiosis varies among organisms, which is reflected by the variability of the gene set associated to the process. Diatoms are unicellular organisms that belong to the Stramenopile clade and have unique life cycles that can include a sexual phase. The exploration of five diatom genomes and one diatom transcriptome led to the identification of 42 genes potentially involved in meiosis. While these include the majority of known meiosis-related genes, several meiosis-specific genes, including DMC1, could not be identified. Furthermore, phylogenetic analyses supported gene identification and revealed ancestral loss and recent expansion in the RAD51 family in diatoms. The two sexual species Pseudo-nitzschia multistriata and Seminavis robusta were used to explore the expression of meiosis-related genes: RAD21, SPO11-2, RAD51-A, RAD51-B and RAD51-C were upregulated during meiosis, whereas other paralogs in these families showed no differential expression patterns, suggesting that they may play a role during vegetative divisions. An almost identical toolkit is shared among Pseudo-nitzschia multiseries and Fragilariopsis cylindrus, as well as two species for which sex has not been observed, Phaeodactylum tricornutum and Thalassiosira pseudonana, suggesting that these two may retain a facultative sexual phase. Our results reveal the conserved meiotic toolkit in six diatom species and indicate that Stramenopiles share major modifications of canonical meiosis processes ancestral to eukaryotes, with important divergences in each Kingdom.
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Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta
BMC Genomics, 2015Co-Authors: Shrikant Patil, Sara Moeys, Peter Von Dassow, Marie J. J. Huysman, Daniel Mapleson, Lieven De Veylder, Remo Sanges, Wim Vyverman, Marina Montresor, Maria Immacolata FerranteAbstract:Background Sexual reproduction is an obligate phase in the life cycle of most eukaryotes. Meiosis varies among organisms, which is reflected by the variability of the gene set associated to the process. Diatoms are unicellular organisms that belong to the Stramenopile clade and have unique life cycles that can include a sexual phase. Results The exploration of five diatom genomes and one diatom transcriptome led to the identification of 42 genes potentially involved in meiosis. While these include the majority of known meiosis-related genes, several meiosis-specific genes, including DMC1, could not be identified. Furthermore, phylogenetic analyses supported gene identification and revealed ancestral loss and recent expansion in the RAD51 family in diatoms. The two sexual species Pseudo-nitzschia multistriata and Seminavis robusta were used to explore the expression of meiosis-related genes: RAD21, SPO11-2, RAD51-A, RAD51-B and RAD51-C were upregulated during meiosis, whereas other paralogs in these families showed no differential expression patterns, suggesting that they may play a role during vegetative divisions. An almost identical toolkit is shared among Pseudo-nitzschia multiseries and Fragilariopsis cylindrus, as well as two species for which sex has not been observed, Phaeodactylum tricornutum and Thalassiosira pseudonana, suggesting that these two may retain a facultative sexual phase. Conclusions Our results reveal the conserved meiotic toolkit in six diatom species and indicate that Stramenopiles share major modifications of canonical meiosis processes ancestral to eukaryotes, with important divergences in each Kingdom.
John D I Harper - One of the best experts on this subject based on the ideXlab platform.
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proteins related to green algal striated fiber assemblin are present in Stramenopiles and alveolates
Protoplasma, 2009Co-Authors: John D I Harper, Jacques Thuet, Karlferdinand Lechtreck, Adrienne R HardhamAbstract:In green algae, striated fiber assemblin (SFA) is the major protein of the striated microtubule-associated fibers that are structural elements in the flagellar basal apparatus. Using Basic Local Alignment Search Tool (BLAST) searches of recently established databases, SFA-like sequences were detected in the genomes not only of green algal species but also of a range of other protists. These included species in two alveolate subgroups, the ciliates (Tetrahymena thermophila, Paramecium tetraurelia) and the dinoflagellates (Perkinsus marinus), and two Stramenopile subgroups, the oomycetes (Phytophthora sojae, Phytophthora ramorum, Phytophthora infestans) and the diatoms (Thalassiosira pseudonana, Phaeodactylum tricornutum). Together with earlier identification of SFA-like sequences in the apicomplexans, these results indicate that homologs of SFA are present across the alveolates and Stramenopiles. Antibodies raised against SFA from the green alga, Spermatozopsis similis, react in immunofluorescence assays with the two basal bodies and an anteriorly directed striated fiber in the flagellar apparatus of biflagellate Phytophthora zoospores.
