The Experts below are selected from a list of 465 Experts worldwide ranked by ideXlab platform
Tobias Eisenberg - One of the best experts on this subject based on the ideXlab platform.
-
infection in a young immunocompetent male caused by Streptobacillus felis a putative zoonotic microorganism transmitted by cats
Clinical Infectious Diseases, 2020Co-Authors: Ulrich Matt, Ahmad Fawzy, Judith Schmiedel, Janina Trauth, Katja Schmidt, Klaus Vogel, Susanne Herold, T Karrasch, Can Imirzalioglu, Tobias EisenbergAbstract:Rat bite fever (RBF) is predominantly caused by Streptobacillus moniliformis. We report a human infection with Streptobacillus felis. Clinical presentation was consistent with RBF, but serologic testing was negative for S. moniliformis. Eventually, S. felis specific sequences were detected in skin lesions of the patient and in the oropharynx of local cats.
-
Streptobacillus canis sp nov isolated from a dog
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Tobias Eisenberg, Ahmad Fawzy, Peter Kampfer, Torsten Semmler, Carsten Heydel, Ellen Prengerberninghoff, Ulrike Kling, Valerij Akimkin, Kristin Muhldorfer, Jochen BlomAbstract:From a phlegmon in a dog an aerobic and facultatively anaerobic, indole-, oxidase- and catalase-negative, non-motile bacterium was isolated in 2019 in Germany that stained Gram-negative and showed a pleomorphic, rod-shaped, non-spore-forming appearance. Based on the results of 16S rRNA gene sequence analyses, strain IHIT1603-19T was assigned to the genus Streptobacillus with sequence similarities of 98.6, 98.0, 97.9, 97.1 and 94.4 % to the type strains of Streptobacillus felis , Streptobacillus notomytis , Streptobacillus ratti , Streptobacillus moniliformis and Streptobacillus hongkongensis , respectively. Strain IHIT1603-19T could also clearly be differentiated from other Streptobacillus species by rpoB, groEL and recA gene, nucleotide and amino acid sequence analyses as well as by core genome phylogeny. Regarding DNA–DNA relatedness, strain IHIT1603-19T demonstrated an average nucleotide identity of 83.00 and 82.28 % compared to S. felis 131000547T and S. moniliformis DSM 12112T, respectively. Chemotaxonomic and physiological data of strain IHIT1603-19T were in congruence with other closely related members of the family Leptotrichiaceae , represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in unequivocally discriminating strain IHIT1603-19T from all currently described taxa of the genus Streptobacillus . On the basis of these data, we propose the novel species Streptobacillus canis sp. nov. with the type strain IHIT1603-19T (=DSM 110501T=CCUG 74118T=CIP 111795T). The G+C content of the DNA of the type strain is 26.6 mol%, genome size is 1.60 Mbp.
-
proposal to reclassify Streptobacillus hongkongensis into a novel genus as pseudoStreptobacillus hongkongensis gen nov comb nov
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Tobias Eisenberg, Stefanie P Glaeser, Jochen Blom, Peter KampferAbstract:The reclassification of Streptobacillus hongkongensis as PseudoStreptobacillus hongkongensis gen. nov., comb. nov. is proposed because of the separate phylogenetic position on the basis of the 16S rRNA gene sequence phylogeny, the combined analysis of the three protein-coding housekeeping genes groEL, gyrB and recA and a core genome sequence phylogeny to all other Streptobacillus species that is supported by phenotypic differences. The species PseudoStreptobacillus hongkongensis is the type species of the genus. The type strain is HKU33T, JCM 18691T, NCTC 13659T, DSM 26322T.
-
Acute Tetraplegia Caused by Rat Bite Fever in Snake Keeper and Transmission of Streptobacillus moniliformis
Emerging Infectious Diseases, 2017Co-Authors: Tobias Eisenberg, Werner Nicklas, Ahmad Fawzy, Simon Poignant, Youenn Jouan, Christa Ewers, Laurent Mereghetti, Antoine GuillonAbstract:We report acute tetraplegia caused by rat bite fever in a 59-year old man (snake keeper) and transmission of Streptobacillus moniliformis. We found an identical characteristic bacterial pattern in rat and human samples, which validated genotyping-based evidence for infection with the same strain, and identified diagnostic difficulties concerning infection with this microorganism.
-
phylogenetic and comparative genomics of the family leptotrichiaceae and introduction of a novel fingerprinting mlva for Streptobacillus moniliformis
BMC Genomics, 2016Co-Authors: Tobias Eisenberg, Werner Nicklas, Ahmad Fawzy, Torsten Semmler, Christa EwersAbstract:Background The Leptotrichiaceae are a family of fairly unnoticed bacteria containing both microbiota on mucous membranes as well as significant pathogens such as Streptobacillus moniliformis, the causative organism of streptobacillary rat bite fever. Comprehensive genomic studies in members of this family have so far not been carried out. We aimed to analyze 47 genomes from 20 different member species to illuminate phylogenetic aspects, as well as genomic and discriminatory properties.
Christa Ewers - One of the best experts on this subject based on the ideXlab platform.
-
Acute Tetraplegia Caused by Rat Bite Fever in Snake Keeper and Transmission of Streptobacillus moniliformis
Emerging Infectious Diseases, 2017Co-Authors: Tobias Eisenberg, Werner Nicklas, Ahmad Fawzy, Simon Poignant, Youenn Jouan, Christa Ewers, Laurent Mereghetti, Antoine GuillonAbstract:We report acute tetraplegia caused by rat bite fever in a 59-year old man (snake keeper) and transmission of Streptobacillus moniliformis. We found an identical characteristic bacterial pattern in rat and human samples, which validated genotyping-based evidence for infection with the same strain, and identified diagnostic difficulties concerning infection with this microorganism.
-
phylogenetic and comparative genomics of the family leptotrichiaceae and introduction of a novel fingerprinting mlva for Streptobacillus moniliformis
BMC Genomics, 2016Co-Authors: Tobias Eisenberg, Werner Nicklas, Ahmad Fawzy, Torsten Semmler, Christa EwersAbstract:Background The Leptotrichiaceae are a family of fairly unnoticed bacteria containing both microbiota on mucous membranes as well as significant pathogens such as Streptobacillus moniliformis, the causative organism of streptobacillary rat bite fever. Comprehensive genomic studies in members of this family have so far not been carried out. We aimed to analyze 47 genomes from 20 different member species to illuminate phylogenetic aspects, as well as genomic and discriminatory properties.
-
Approved and novel strategies in diagnostics of rat bite fever and other Streptobacillus infections in humans and animals
Virulence, 2016Co-Authors: Eisenberg T, Christa Ewers, Rau J, Akimkin, Nicklas WAbstract:Rat bite fever (RBF), a worldwide occurring and most likely under-diagnosed zoonosis caused by Streptobacillus moniliformis, represents the most prominent disease of Streptobacillus infections. Recently, novel members have been described, from which a reservoir in rats and other animal species and a zoonotic potential can be assumed. Despite regularly published case reports, diagnostics of RBF continues to represent a 'diagnostic dilemma', because the mostly applied 16S rRNA sequence analysis may be uncertain for proper pathogen identification. Virtually nothing is known regarding prevalence in humans and animal reservoirs. For a realistic assessment of the pathogen's spread, epidemiology and virulence traits, future studies should focus on the genomic background of Streptobacillus. Full genome sequence analyses of a representative collection of strains might facilitate to unequivocally identify and type isolates. Prevalence studies using selective enrichment mechanisms may also enable the isolation of novel strains and candidate species of this neglected group of microorganisms.
-
Streptobacillus ratti sp nov isolated from a black rat rattus rattus
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Tobias Eisenberg, Tsutomu Tanikawa, Werner Nicklas, Koichi Imaoka, Masanobu Kimura, Christa Ewers, Stefanie P Glaeser, Torsten Semmler, Peter KampferAbstract:An indole-, oxidase- and catalase-negative, non-motile bacterium, strain OGS16T, was isolated from an oral swab of a feral black rat (Rattus rattus) in 2007 in Japan. It stained Gram-negative and had pleomorphic, rod-shaped, non-spore-forming cells. Based on 16S rRNA gene sequence analyses, strain OGS16T was assigned to the genus Streptobacillus, with 16S rRNA gene sequence similarities of 99.3, 99.0, 98.6 and 95.5 % to the type strains of Streptobacillus moniliformis, Streptobacillus notomytis, Streptobacillus felis and Streptobacillus hongkongensis, respectively. Strain OGS16T could also be differentiated clearly from other species of the genus Streptobacillus by rpoB, groEL and recA nucleotide and deduced amino acid sequence analysis. DNA–DNA relatedness as obtained by average nucleotide identity was 89.10 % between strain OGS16T and Streptobacillus moniliformis DSM 12112T. Chemotaxonomic and physiological data for strain OGS16T were congruent with results for other closely related members of the family Leptotrichiaceae, represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in discriminating strain OGS16T unequivocally from all currently described taxa of the genus Streptobacillus. On the basis of these data, we propose the novel species Streptobacillus ratti sp. nov., with the type strain OGS16T ( = JCM 31098T = DSM 101843T). The G+C content of the DNA of the type strain is 25.9 mol% and the genome size is 1.50 Mbp.
-
Streptobacillus notomytis sp nov isolated from a spinifex hopping mouse notomys alexis thomas 1922 and emended description of Streptobacillus levaditi et al 1925 eisenberg et al 2015 emend
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Tobias Eisenberg, Werner Nicklas, Koichi Imaoka, Christa Ewers, Stefanie P Glaeser, Norman Mauder, Torsten Semmler, Jorg Rau, Nicola Hofmann, Masanobu KimuraAbstract:A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was isolated in 1979 from the heart of a spinifex hopping mouse (Notomys alexis Thomas, 1922) with septicaemia and stored as Streptobacillus moniliformis in the strain collection of the Animal Health Laboratory, South Perth, Western Australia (AHL 370-1), as well as under CCUG 12425. On the basis of 16S rRNA gene sequence analyses, the strain was assigned to the genus Streptobacillus, with 99.4 % sequence similarity to the type strain of Streptobacillus moniliformis, 95.6 % sequence similarity to the type strain of Streptobacillus hongkongensis and 99.0 % sequence similarity to the type strain of Streptobacillus felis. The clear differentiation of strain AHL 370-1T from Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis was also supported by rpoB, groEL and recA nucleotide and amino acid sequence analysis. Average nucleotide identity was 87.16 % between strain AHL 370-1T and Streptobacillus moniliformis DSM 12112T. Physiological data confirmed the allocation of strain AHL 370-1T to the family Leptotrichiaceae, considering the very similar profiles of enzyme activities and fatty acids compared to closely related species. Within the genus Streptobacillus, isolate AHL 370-1T could also be separated unambiguously from the type strains of Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis by MALDI-TOF mass spectrometry. Two further strains (KWG2 and KWG24) isolated from asymptomatic black rats in Japan were highly similar to AHL 370-1T. On the basis of these data, we propose the novel species Streptobacillus notomytis sp. nov., with the type strain AHL 370-1T ( = CCUG 12425T = DSM 100026T = CCM 8593T = EF 12425T).
Werner Nicklas - One of the best experts on this subject based on the ideXlab platform.
-
Acute Tetraplegia Caused by Rat Bite Fever in Snake Keeper and Transmission of Streptobacillus moniliformis
Emerging Infectious Diseases, 2017Co-Authors: Tobias Eisenberg, Werner Nicklas, Ahmad Fawzy, Simon Poignant, Youenn Jouan, Christa Ewers, Laurent Mereghetti, Antoine GuillonAbstract:We report acute tetraplegia caused by rat bite fever in a 59-year old man (snake keeper) and transmission of Streptobacillus moniliformis. We found an identical characteristic bacterial pattern in rat and human samples, which validated genotyping-based evidence for infection with the same strain, and identified diagnostic difficulties concerning infection with this microorganism.
-
phylogenetic and comparative genomics of the family leptotrichiaceae and introduction of a novel fingerprinting mlva for Streptobacillus moniliformis
BMC Genomics, 2016Co-Authors: Tobias Eisenberg, Werner Nicklas, Ahmad Fawzy, Torsten Semmler, Christa EwersAbstract:Background The Leptotrichiaceae are a family of fairly unnoticed bacteria containing both microbiota on mucous membranes as well as significant pathogens such as Streptobacillus moniliformis, the causative organism of streptobacillary rat bite fever. Comprehensive genomic studies in members of this family have so far not been carried out. We aimed to analyze 47 genomes from 20 different member species to illuminate phylogenetic aspects, as well as genomic and discriminatory properties.
-
Streptobacillus ratti sp nov isolated from a black rat rattus rattus
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Tobias Eisenberg, Tsutomu Tanikawa, Werner Nicklas, Koichi Imaoka, Masanobu Kimura, Christa Ewers, Stefanie P Glaeser, Torsten Semmler, Peter KampferAbstract:An indole-, oxidase- and catalase-negative, non-motile bacterium, strain OGS16T, was isolated from an oral swab of a feral black rat (Rattus rattus) in 2007 in Japan. It stained Gram-negative and had pleomorphic, rod-shaped, non-spore-forming cells. Based on 16S rRNA gene sequence analyses, strain OGS16T was assigned to the genus Streptobacillus, with 16S rRNA gene sequence similarities of 99.3, 99.0, 98.6 and 95.5 % to the type strains of Streptobacillus moniliformis, Streptobacillus notomytis, Streptobacillus felis and Streptobacillus hongkongensis, respectively. Strain OGS16T could also be differentiated clearly from other species of the genus Streptobacillus by rpoB, groEL and recA nucleotide and deduced amino acid sequence analysis. DNA–DNA relatedness as obtained by average nucleotide identity was 89.10 % between strain OGS16T and Streptobacillus moniliformis DSM 12112T. Chemotaxonomic and physiological data for strain OGS16T were congruent with results for other closely related members of the family Leptotrichiaceae, represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in discriminating strain OGS16T unequivocally from all currently described taxa of the genus Streptobacillus. On the basis of these data, we propose the novel species Streptobacillus ratti sp. nov., with the type strain OGS16T ( = JCM 31098T = DSM 101843T). The G+C content of the DNA of the type strain is 25.9 mol% and the genome size is 1.50 Mbp.
-
Streptobacillus notomytis sp nov isolated from a spinifex hopping mouse notomys alexis thomas 1922 and emended description of Streptobacillus levaditi et al 1925 eisenberg et al 2015 emend
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Tobias Eisenberg, Werner Nicklas, Koichi Imaoka, Christa Ewers, Stefanie P Glaeser, Norman Mauder, Torsten Semmler, Jorg Rau, Nicola Hofmann, Masanobu KimuraAbstract:A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was isolated in 1979 from the heart of a spinifex hopping mouse (Notomys alexis Thomas, 1922) with septicaemia and stored as Streptobacillus moniliformis in the strain collection of the Animal Health Laboratory, South Perth, Western Australia (AHL 370-1), as well as under CCUG 12425. On the basis of 16S rRNA gene sequence analyses, the strain was assigned to the genus Streptobacillus, with 99.4 % sequence similarity to the type strain of Streptobacillus moniliformis, 95.6 % sequence similarity to the type strain of Streptobacillus hongkongensis and 99.0 % sequence similarity to the type strain of Streptobacillus felis. The clear differentiation of strain AHL 370-1T from Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis was also supported by rpoB, groEL and recA nucleotide and amino acid sequence analysis. Average nucleotide identity was 87.16 % between strain AHL 370-1T and Streptobacillus moniliformis DSM 12112T. Physiological data confirmed the allocation of strain AHL 370-1T to the family Leptotrichiaceae, considering the very similar profiles of enzyme activities and fatty acids compared to closely related species. Within the genus Streptobacillus, isolate AHL 370-1T could also be separated unambiguously from the type strains of Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis by MALDI-TOF mass spectrometry. Two further strains (KWG2 and KWG24) isolated from asymptomatic black rats in Japan were highly similar to AHL 370-1T. On the basis of these data, we propose the novel species Streptobacillus notomytis sp. nov., with the type strain AHL 370-1T ( = CCUG 12425T = DSM 100026T = CCM 8593T = EF 12425T).
-
Streptobacillus felis sp nov isolated from a cat with pneumonia and emended descriptions of the genus Streptobacillus and of Streptobacillus moniliformis
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Tobias Eisenberg, Werner Nicklas, Stefanie P Glaeser, Norman Mauder, Matthias Contzen, Khayrieh Aledelbi, Peter KampferAbstract:A pleomorphic, Gram-stain-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium (strain 131000547T) was isolated from the lungs of a cat with pneumonia. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the genus Streptobacillus with 97.6 % sequence similarity to the type strain of Streptobacillus moniliformis and 94.6 % to that of Streptobacillus hongkongensis. The clear differentiation of strain 131000547T from Streptobacillus moniliformis and Streptobacillus hongkongensis was also supported by gyrB, groEL, and recA nucleotide and amino acid sequence analysis. DNA–DNA hybridization demonstrated ≤ 19.9 % (reciprocal 28.7 %) DNA–DNA relatedness between strain 131000547T and Streptobacillus moniliformis DSM 12112T. Physiological data confirmed the allocation of strain 131000547T to the family Leptotrichiaceae. Strain 131000547T has a unique profile of enzyme activities allowing differentiation from the most closely related species. Within the genus Streptobacillus, isolate 131000547T could also unambiguously be separated from Streptobacillus moniliformis and Streptobacillus hongkongensis by both matrix-assisted laser desorption ionization time-of-flight mass spectrometry and Fourier transform-infrared spectroscopy. On the basis of these data, a novel species of the genus Streptobacillus, Streptobacillus felis sp. nov., is proposed with the type strain 131000547T ( = DSM 29248T = CCUG 66203T = CCM 8542T). Emended descriptions of the genus Streptobacillus and of Streptobacillus moniliformis are also given.
Torsten Semmler - One of the best experts on this subject based on the ideXlab platform.
-
Streptobacillus canis sp nov isolated from a dog
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Tobias Eisenberg, Ahmad Fawzy, Peter Kampfer, Torsten Semmler, Carsten Heydel, Ellen Prengerberninghoff, Ulrike Kling, Valerij Akimkin, Kristin Muhldorfer, Jochen BlomAbstract:From a phlegmon in a dog an aerobic and facultatively anaerobic, indole-, oxidase- and catalase-negative, non-motile bacterium was isolated in 2019 in Germany that stained Gram-negative and showed a pleomorphic, rod-shaped, non-spore-forming appearance. Based on the results of 16S rRNA gene sequence analyses, strain IHIT1603-19T was assigned to the genus Streptobacillus with sequence similarities of 98.6, 98.0, 97.9, 97.1 and 94.4 % to the type strains of Streptobacillus felis , Streptobacillus notomytis , Streptobacillus ratti , Streptobacillus moniliformis and Streptobacillus hongkongensis , respectively. Strain IHIT1603-19T could also clearly be differentiated from other Streptobacillus species by rpoB, groEL and recA gene, nucleotide and amino acid sequence analyses as well as by core genome phylogeny. Regarding DNA–DNA relatedness, strain IHIT1603-19T demonstrated an average nucleotide identity of 83.00 and 82.28 % compared to S. felis 131000547T and S. moniliformis DSM 12112T, respectively. Chemotaxonomic and physiological data of strain IHIT1603-19T were in congruence with other closely related members of the family Leptotrichiaceae , represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in unequivocally discriminating strain IHIT1603-19T from all currently described taxa of the genus Streptobacillus . On the basis of these data, we propose the novel species Streptobacillus canis sp. nov. with the type strain IHIT1603-19T (=DSM 110501T=CCUG 74118T=CIP 111795T). The G+C content of the DNA of the type strain is 26.6 mol%, genome size is 1.60 Mbp.
-
phylogenetic and comparative genomics of the family leptotrichiaceae and introduction of a novel fingerprinting mlva for Streptobacillus moniliformis
BMC Genomics, 2016Co-Authors: Tobias Eisenberg, Werner Nicklas, Ahmad Fawzy, Torsten Semmler, Christa EwersAbstract:Background The Leptotrichiaceae are a family of fairly unnoticed bacteria containing both microbiota on mucous membranes as well as significant pathogens such as Streptobacillus moniliformis, the causative organism of streptobacillary rat bite fever. Comprehensive genomic studies in members of this family have so far not been carried out. We aimed to analyze 47 genomes from 20 different member species to illuminate phylogenetic aspects, as well as genomic and discriminatory properties.
-
Streptobacillus ratti sp nov isolated from a black rat rattus rattus
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Tobias Eisenberg, Tsutomu Tanikawa, Werner Nicklas, Koichi Imaoka, Masanobu Kimura, Christa Ewers, Stefanie P Glaeser, Torsten Semmler, Peter KampferAbstract:An indole-, oxidase- and catalase-negative, non-motile bacterium, strain OGS16T, was isolated from an oral swab of a feral black rat (Rattus rattus) in 2007 in Japan. It stained Gram-negative and had pleomorphic, rod-shaped, non-spore-forming cells. Based on 16S rRNA gene sequence analyses, strain OGS16T was assigned to the genus Streptobacillus, with 16S rRNA gene sequence similarities of 99.3, 99.0, 98.6 and 95.5 % to the type strains of Streptobacillus moniliformis, Streptobacillus notomytis, Streptobacillus felis and Streptobacillus hongkongensis, respectively. Strain OGS16T could also be differentiated clearly from other species of the genus Streptobacillus by rpoB, groEL and recA nucleotide and deduced amino acid sequence analysis. DNA–DNA relatedness as obtained by average nucleotide identity was 89.10 % between strain OGS16T and Streptobacillus moniliformis DSM 12112T. Chemotaxonomic and physiological data for strain OGS16T were congruent with results for other closely related members of the family Leptotrichiaceae, represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in discriminating strain OGS16T unequivocally from all currently described taxa of the genus Streptobacillus. On the basis of these data, we propose the novel species Streptobacillus ratti sp. nov., with the type strain OGS16T ( = JCM 31098T = DSM 101843T). The G+C content of the DNA of the type strain is 25.9 mol% and the genome size is 1.50 Mbp.
-
Streptobacillus notomytis sp nov isolated from a spinifex hopping mouse notomys alexis thomas 1922 and emended description of Streptobacillus levaditi et al 1925 eisenberg et al 2015 emend
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Tobias Eisenberg, Werner Nicklas, Koichi Imaoka, Christa Ewers, Stefanie P Glaeser, Norman Mauder, Torsten Semmler, Jorg Rau, Nicola Hofmann, Masanobu KimuraAbstract:A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was isolated in 1979 from the heart of a spinifex hopping mouse (Notomys alexis Thomas, 1922) with septicaemia and stored as Streptobacillus moniliformis in the strain collection of the Animal Health Laboratory, South Perth, Western Australia (AHL 370-1), as well as under CCUG 12425. On the basis of 16S rRNA gene sequence analyses, the strain was assigned to the genus Streptobacillus, with 99.4 % sequence similarity to the type strain of Streptobacillus moniliformis, 95.6 % sequence similarity to the type strain of Streptobacillus hongkongensis and 99.0 % sequence similarity to the type strain of Streptobacillus felis. The clear differentiation of strain AHL 370-1T from Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis was also supported by rpoB, groEL and recA nucleotide and amino acid sequence analysis. Average nucleotide identity was 87.16 % between strain AHL 370-1T and Streptobacillus moniliformis DSM 12112T. Physiological data confirmed the allocation of strain AHL 370-1T to the family Leptotrichiaceae, considering the very similar profiles of enzyme activities and fatty acids compared to closely related species. Within the genus Streptobacillus, isolate AHL 370-1T could also be separated unambiguously from the type strains of Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis by MALDI-TOF mass spectrometry. Two further strains (KWG2 and KWG24) isolated from asymptomatic black rats in Japan were highly similar to AHL 370-1T. On the basis of these data, we propose the novel species Streptobacillus notomytis sp. nov., with the type strain AHL 370-1T ( = CCUG 12425T = DSM 100026T = CCM 8593T = EF 12425T).
-
Physiological characteristics of field isolates and reference strains from Streptobacillus moniliformis and of reference strains from Streptobacillus felis 131000547T, Streptobacillus hongkongensis DSM 26322T and Sebaldella termitidis NCTC 11300T.
2015Co-Authors: Tobias Eisenberg, Werner Nicklas, Norman Mauder, Matthias Contzen, Khayrieh Aledelbi, Torsten Semmler, Jorg Rau, Nicola Hofmann, Christa EwersAbstract:Physiological characteristics were obtained by an individual panel of eleven# discriminatory reactions designed for the identification of Streptobacillus spp. (Micronaut Strep2 and RPO; all Merlin Diagnostika GmbH)*, VITEK2-compact with the NHI card†, API-ZYM‡ (both bioMeriéux) and haemolytic properties on Columbia agar with 5% sheep blood; for congruent results see text; +: positive;-: negative; w: weak reaction;§ potential discriminatory character for species identificationPhysiological characteristics of field isolates and reference strains from Streptobacillus moniliformis and of reference strains from Streptobacillus felis 131000547T, Streptobacillus hongkongensis DSM 26322T and Sebaldella termitidis NCTC 11300T.
Peter Kampfer - One of the best experts on this subject based on the ideXlab platform.
-
Streptobacillus canis sp nov isolated from a dog
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Tobias Eisenberg, Ahmad Fawzy, Peter Kampfer, Torsten Semmler, Carsten Heydel, Ellen Prengerberninghoff, Ulrike Kling, Valerij Akimkin, Kristin Muhldorfer, Jochen BlomAbstract:From a phlegmon in a dog an aerobic and facultatively anaerobic, indole-, oxidase- and catalase-negative, non-motile bacterium was isolated in 2019 in Germany that stained Gram-negative and showed a pleomorphic, rod-shaped, non-spore-forming appearance. Based on the results of 16S rRNA gene sequence analyses, strain IHIT1603-19T was assigned to the genus Streptobacillus with sequence similarities of 98.6, 98.0, 97.9, 97.1 and 94.4 % to the type strains of Streptobacillus felis , Streptobacillus notomytis , Streptobacillus ratti , Streptobacillus moniliformis and Streptobacillus hongkongensis , respectively. Strain IHIT1603-19T could also clearly be differentiated from other Streptobacillus species by rpoB, groEL and recA gene, nucleotide and amino acid sequence analyses as well as by core genome phylogeny. Regarding DNA–DNA relatedness, strain IHIT1603-19T demonstrated an average nucleotide identity of 83.00 and 82.28 % compared to S. felis 131000547T and S. moniliformis DSM 12112T, respectively. Chemotaxonomic and physiological data of strain IHIT1603-19T were in congruence with other closely related members of the family Leptotrichiaceae , represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in unequivocally discriminating strain IHIT1603-19T from all currently described taxa of the genus Streptobacillus . On the basis of these data, we propose the novel species Streptobacillus canis sp. nov. with the type strain IHIT1603-19T (=DSM 110501T=CCUG 74118T=CIP 111795T). The G+C content of the DNA of the type strain is 26.6 mol%, genome size is 1.60 Mbp.
-
proposal to reclassify Streptobacillus hongkongensis into a novel genus as pseudoStreptobacillus hongkongensis gen nov comb nov
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Tobias Eisenberg, Stefanie P Glaeser, Jochen Blom, Peter KampferAbstract:The reclassification of Streptobacillus hongkongensis as PseudoStreptobacillus hongkongensis gen. nov., comb. nov. is proposed because of the separate phylogenetic position on the basis of the 16S rRNA gene sequence phylogeny, the combined analysis of the three protein-coding housekeeping genes groEL, gyrB and recA and a core genome sequence phylogeny to all other Streptobacillus species that is supported by phenotypic differences. The species PseudoStreptobacillus hongkongensis is the type species of the genus. The type strain is HKU33T, JCM 18691T, NCTC 13659T, DSM 26322T.
-
Streptobacillus ratti sp nov isolated from a black rat rattus rattus
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Tobias Eisenberg, Tsutomu Tanikawa, Werner Nicklas, Koichi Imaoka, Masanobu Kimura, Christa Ewers, Stefanie P Glaeser, Torsten Semmler, Peter KampferAbstract:An indole-, oxidase- and catalase-negative, non-motile bacterium, strain OGS16T, was isolated from an oral swab of a feral black rat (Rattus rattus) in 2007 in Japan. It stained Gram-negative and had pleomorphic, rod-shaped, non-spore-forming cells. Based on 16S rRNA gene sequence analyses, strain OGS16T was assigned to the genus Streptobacillus, with 16S rRNA gene sequence similarities of 99.3, 99.0, 98.6 and 95.5 % to the type strains of Streptobacillus moniliformis, Streptobacillus notomytis, Streptobacillus felis and Streptobacillus hongkongensis, respectively. Strain OGS16T could also be differentiated clearly from other species of the genus Streptobacillus by rpoB, groEL and recA nucleotide and deduced amino acid sequence analysis. DNA–DNA relatedness as obtained by average nucleotide identity was 89.10 % between strain OGS16T and Streptobacillus moniliformis DSM 12112T. Chemotaxonomic and physiological data for strain OGS16T were congruent with results for other closely related members of the family Leptotrichiaceae, represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in discriminating strain OGS16T unequivocally from all currently described taxa of the genus Streptobacillus. On the basis of these data, we propose the novel species Streptobacillus ratti sp. nov., with the type strain OGS16T ( = JCM 31098T = DSM 101843T). The G+C content of the DNA of the type strain is 25.9 mol% and the genome size is 1.50 Mbp.
-
Streptobacillus felis sp nov isolated from a cat with pneumonia and emended descriptions of the genus Streptobacillus and of Streptobacillus moniliformis
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Tobias Eisenberg, Werner Nicklas, Stefanie P Glaeser, Norman Mauder, Matthias Contzen, Khayrieh Aledelbi, Peter KampferAbstract:A pleomorphic, Gram-stain-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium (strain 131000547T) was isolated from the lungs of a cat with pneumonia. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the genus Streptobacillus with 97.6 % sequence similarity to the type strain of Streptobacillus moniliformis and 94.6 % to that of Streptobacillus hongkongensis. The clear differentiation of strain 131000547T from Streptobacillus moniliformis and Streptobacillus hongkongensis was also supported by gyrB, groEL, and recA nucleotide and amino acid sequence analysis. DNA–DNA hybridization demonstrated ≤ 19.9 % (reciprocal 28.7 %) DNA–DNA relatedness between strain 131000547T and Streptobacillus moniliformis DSM 12112T. Physiological data confirmed the allocation of strain 131000547T to the family Leptotrichiaceae. Strain 131000547T has a unique profile of enzyme activities allowing differentiation from the most closely related species. Within the genus Streptobacillus, isolate 131000547T could also unambiguously be separated from Streptobacillus moniliformis and Streptobacillus hongkongensis by both matrix-assisted laser desorption ionization time-of-flight mass spectrometry and Fourier transform-infrared spectroscopy. On the basis of these data, a novel species of the genus Streptobacillus, Streptobacillus felis sp. nov., is proposed with the type strain 131000547T ( = DSM 29248T = CCUG 66203T = CCM 8542T). Emended descriptions of the genus Streptobacillus and of Streptobacillus moniliformis are also given.
