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Zoilo Pires De Camargo - One of the best experts on this subject based on the ideXlab platform.
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genetic diversity and antifungal susceptibility profiles in causative agents of sporotrichosis
BMC Infectious Diseases, 2014Co-Authors: Anderson Messias Rodrigues, Sybren G De Hoog, Debora De Cassia Pires, Raimunda Sâmia Nogueira Brihante, Jose Julio Costa Sidrim, Marcos Fabio Gadelha, Arnaldo Lopes Colombo, Zoilo Pires De CamargoAbstract:Background Sporotrichosis is a chronic Subcutaneous Mycosis of humans and animals, which is typically acquired by traumatic inoculation of plant material contaminated with Sporothrix propagules, or via animals, mainly felines. Sporothrix infections notably occur in outbreaks, with large epidemics currently taking place in southeastern Brazil and northeastern China. Pathogenic species include Sporothrix brasiliensis, Sporothrix schenckii s. str., Sporothrix globosa, and Sporothrix luriei, which exhibit differing geographical distribution, virulence, and resistance to antifungals. The phylogenetically remote species Sporothrix mexicana also shows a mild pathogenic potential.
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Genotyping species of the Sporothrix schenckii complex by PCR-RFLP of calmodulin.
Diagnostic Microbiology and Infectious Disease, 2014Co-Authors: Anderson Messias Rodrigues, G. Sybren De Hoog, Zoilo Pires De CamargoAbstract:Sporotrichosis is one of the most common Subcutaneous Mycosis in Latin America and is caused by 4 pathogenic thermodimorphic fungi in the genus Sporothrix. From both therapeutic and epidemiological perspectives, it is essential to identify the causative agents down to the species level. Traditional parameters may overlap among closely related species, and we propose polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as an alternative approach. In the present study, the calmodulin gene was amplified and digested with HhaI to yield 5 different electrophoretic patterns representing all medically important Sporothrix species: Sporothrix brasiliensis, Sporothrix schenckii sensu stricto, Sporothrix globosa, and Sporothrix luriei. The PCR-RFLP protocol described here is a simple and inexpensive method and is highly suitable for accurate routine genotyping of relevant Sporothrix species.
Roberto Arenas - One of the best experts on this subject based on the ideXlab platform.
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ChromoblastoMycosis.
Clinics in dermatology, 2012Co-Authors: Edoardo Torres-guerrero, Rafael Isa-isa, Mariel Isa, Roberto ArenasAbstract:ChromoblastoMycosis is a chronic, Subcutaneous Mycosis, characterized by verrucous nodular lesions, usually involving the legs and mainly caused by Fonsecaea, Phialophora, and Cladophialophora spp. The characteristic finding on direct examination or biopsy specimen is the presence of fumagoid cells or Medlar bodies. ChromoblastoMycosis can be refractory to medical treatment. Therapeutic options include oral itraconazole, terbinafine, or 5-fluocytosine, alone or combined with surgery or cryosurgery.
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Rhinofacial conidioboloMycosis (entomophthoraMycosis)
Clinics in dermatology, 2012Co-Authors: Rafael Isa-isa, Roberto Arenas, Ramón Fernández, Mariel IsaAbstract:ConidioboloMycosis is a tropical rhinofacial Subcutaneous Mycosis caused by Conidiobolus coronatus. It is characterized histopathologically by the presence of hyphae surrounded by an eosinophilic halo, the so-called Splendore-Hoeppli phenomenon. There is no standard treatment, but itraconazole is an effective option.
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Sporotrichosis.
Clinics in dermatology, 2012Co-Authors: Elsa Vásquez-del-mercado, Roberto Arenas, Carmen Padilla-desgarenesAbstract:Sporotrichosis is the most common Subcutaneous Mycosis. It is usually acquired by traumatic inoculation, and it is caused by one of the species of the Sporothrix schenckii complex. More than 6 species, such as S schenckii sensu stricto, Sporothrix brasiliensis, Sporothrix globosa, Sporothrix mexicana, and Sporothrix albicans, have been identified by molecular techniques. The most common presentation is cutaneous disease, which is classified into fixed and lymphocutaneous forms. Osteoarticular, pulmonary, mucosal, disseminated, and systemic infections are less common and usually occur in immunosuppressed individuals. The diagnosis is suggested by biopsy specimen and confirmed by tissue culture. Itraconazole is considered the treatment of choice, although in some undeveloped countries potassium iodide is still used, owing to its safety and low cost. For systemic or disseminated cases, amphotericin B is the treatment of choice.
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Carcinoma epidermoide como complicação letal de lesões crônicas de cromoblastomicose
Anais brasileiros de dermatologia, 2010Co-Authors: Edoardo Torres, Javier Gil Beristain, Zahide Lievanos, Roberto ArenasAbstract:ChromoblastoMycosis is a Subcutaneous Mycosis caused by the dermatophytic fungi Fonsecaea, Phialophora and Cladophialophora. Usual complications include secondary infection, lymphedema and elephantiasis. Associated malignancies have been found in chronic cases. This case report describes a 72 year-old male with a 30 year history of chromoblastoMycosis in the gluteal region, who went on to develop a squamous cell carcinoma.
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Sporothrix schenckii type 3D (mtDNA-RFLP): report of an osteoarticular case.
The Journal of dermatology, 2006Co-Authors: Pablo Campos-macías, Roberto Arenas, Me Vega‐memije, Masako KawasakiAbstract:Sporotrichosis is a frequent Subcutaneous Mycosis in Mexico and lymphocutaneous cases are the most common type. Extracutaneous or disseminated forms are exceptional and are usually seen in immunosuppressed hosts. We report the case of a 74-year-old immunocompetent male with osteoarticular involvement. The isolated Sporothrix schenckii was classified as type 3D according to restriction fragment length polymorphism analysis of the mitochondrial DNA (mtDNA-RFLP).
Anderson Messias Rodrigues - One of the best experts on this subject based on the ideXlab platform.
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genetic diversity and antifungal susceptibility profiles in causative agents of sporotrichosis
BMC Infectious Diseases, 2014Co-Authors: Anderson Messias Rodrigues, Sybren G De Hoog, Debora De Cassia Pires, Raimunda Sâmia Nogueira Brihante, Jose Julio Costa Sidrim, Marcos Fabio Gadelha, Arnaldo Lopes Colombo, Zoilo Pires De CamargoAbstract:Background Sporotrichosis is a chronic Subcutaneous Mycosis of humans and animals, which is typically acquired by traumatic inoculation of plant material contaminated with Sporothrix propagules, or via animals, mainly felines. Sporothrix infections notably occur in outbreaks, with large epidemics currently taking place in southeastern Brazil and northeastern China. Pathogenic species include Sporothrix brasiliensis, Sporothrix schenckii s. str., Sporothrix globosa, and Sporothrix luriei, which exhibit differing geographical distribution, virulence, and resistance to antifungals. The phylogenetically remote species Sporothrix mexicana also shows a mild pathogenic potential.
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Genotyping species of the Sporothrix schenckii complex by PCR-RFLP of calmodulin.
Diagnostic Microbiology and Infectious Disease, 2014Co-Authors: Anderson Messias Rodrigues, G. Sybren De Hoog, Zoilo Pires De CamargoAbstract:Sporotrichosis is one of the most common Subcutaneous Mycosis in Latin America and is caused by 4 pathogenic thermodimorphic fungi in the genus Sporothrix. From both therapeutic and epidemiological perspectives, it is essential to identify the causative agents down to the species level. Traditional parameters may overlap among closely related species, and we propose polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as an alternative approach. In the present study, the calmodulin gene was amplified and digested with HhaI to yield 5 different electrophoretic patterns representing all medically important Sporothrix species: Sporothrix brasiliensis, Sporothrix schenckii sensu stricto, Sporothrix globosa, and Sporothrix luriei. The PCR-RFLP protocol described here is a simple and inexpensive method and is highly suitable for accurate routine genotyping of relevant Sporothrix species.
Julio C. Villagómez-castro - One of the best experts on this subject based on the ideXlab platform.
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Analysis of biofilm formation by Sporothrix schenckii
Medical mycology, 2020Co-Authors: Rocío Sánchez-herrera, Lérida Liss Flores-villavicencio, J. L. Pichardo-molina, Jose Pedro Castruita-dominguez, Xochilt Aparicio-fernández, Myrna Sabanero López, Julio C. Villagómez-castroAbstract:The development of mature biofilms is an aid in numerous aspects of the life cycle of fungi. It is well known that Sporothrix schenckii complex causes a benign Subcutaneous Mycosis, but recent studies have suggestedthat biofilm formation may be one of the important factors involved in its virulence. Here we report the study of the biomass organization and a model of the stages of S. schenckii biofilm development: adsorption, active adhesion, microcolony formation, maturation, and dispersal of biofilm fragments. During the development, the biofilm is surrounded by extracellular matrix, which contains glycoprotein (mannose rich), carbohydrates, lipids, and nucleic acid. In addition, the extracellular DNA increases in extracellular matrix as a key component to structural integrity and antifungal resistance. The study of S. schenckii biofilm contributes to a better understanding of growth biofilm and physiology, adding new insights into the mechanisms of virulence and persistence of pathogenic microorganisms.
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Sporothrix schenckii complex and sporotrichosis, an emerging health problem.
Future Microbiology, 2011Co-Authors: Everardo López-romero, María Del Rocío Reyes-montes, Armando Pérez-torres, Estela Ruiz-baca, Julio C. Villagómez-castro, Héctor M. Mora-montes, Arturo Flores-carreón, Conchita TorielloAbstract:Sporothrix schenckii, now named the S. schenckii species complex, has largely been known as the etiological agent of sporotrichosis, which is an acute or chronic Subcutaneous Mycosis of humans and other mammals. Gene sequencing has revealed the following species in the S. schenckii complex: Sporothrix albicans, Sporothrix brasiliensis, Sporothrix globosa, Sporothrix luriei, Sporothrix mexicana and S. schenckii. The increasing number of reports of Sporothrix infection in immunocompromised patients, mainly the HIV-infected population, suggests sporotrichosis as an emerging global health problem concomitant with the AIDS pandemic. Molecular studies have demonstrated a high level of intraspecific variability. Components of the S. schenckii cell wall that act as adhesins and immunogenic inducers, such as a 70-kDa glycoprotein, are apparently specific to this fungus. The main glycan peptidorhamnomannan cell wall component is the only O-linked glycan structure known in S. schenckii. It contains an α-mannobiose c...
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Isolation and some properties of a glycoprotein of 70 kDa (Gp70) from the cell wall of Sporothrix schenckii involved in fungal adherence to dermal extracellular matrix
Medical mycology, 2008Co-Authors: Estela Ruiz-baca, Armando Pérez-torres, Julio C. Villagómez-castro, Conchita Toriello, Myrna Sabanero-lópez, Everardo López-romeroAbstract:Sporothrix schenckii is the etiological agent of sporotrichosis, a Subcutaneous Mycosis and an emerging disease in immunocompromised patients. Adherence to target cells is a prerequisite for fungal dissemination and systemic complications. However, information on the cell surface components involved in this interaction is rather scarce. In this investigation, the extraction of isolated cell walls from the yeast phase of S. schenckii with SDS and separation of proteins by SDS-PAGE led to the identification of a periodic acid-Schiff (PAS)-reacting 70 kDa glycoprotein (Gp70) that was purified by elution from electrophoresis gels. The purified glycopeptide exhibited a pI of 4.1 and about 5.7% of its molecular mass was contributed by N-linked glycans with no evidence for O-linked oligosaccharides. Confocal analysis of immunofluorescence assays with polyclonal antibodies directed towards Gp70 revealed a rather uniform distribution of the antigen at the cell surface with no distinguishable differences among thre...
Everardo López-romero - One of the best experts on this subject based on the ideXlab platform.
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Cell wall proteins of Sporothrix schenckii as immunoprotective agents.
Revista iberoamericana de micologia, 2013Co-Authors: Carlos A. Alba-fierro, Everardo López-romero, Armando Pérez-torres, Mayra Cuéllar-cruz, Estela Ruiz-bacaAbstract:Sporothrix schenckii is the etiological agent of sporotrichosis, an endemic Subcutaneous Mycosis in Latin America. Cell wall (CW) proteins located on the cell surface are inducers of cellular and humoral immune responses, potential candidates for diagnosis purposes and to generate vaccines to prevent fungal infections. This mini-review emphasizes the potential use of S. schenckii CW proteins as protective and therapeutic immune response inducers against sporotrichosis. A number of pathogenic fungi display CW components that have been characterized as inducers of protective cellular and humoral immune responses against the whole pathogen from which they were originally purified. The isolation and characterization of immunodominant protein components of the CW of S. schenckii have become relevant because of their potential in the development of protective and therapeutic immune responses against sporotrichosis. This manuscript is part of the series of works presented at the "V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi" (Oaxaca, Mexico, 2012).
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Sporothrix schenckii complex and sporotrichosis, an emerging health problem.
Future Microbiology, 2011Co-Authors: Everardo López-romero, María Del Rocío Reyes-montes, Armando Pérez-torres, Estela Ruiz-baca, Julio C. Villagómez-castro, Héctor M. Mora-montes, Arturo Flores-carreón, Conchita TorielloAbstract:Sporothrix schenckii, now named the S. schenckii species complex, has largely been known as the etiological agent of sporotrichosis, which is an acute or chronic Subcutaneous Mycosis of humans and other mammals. Gene sequencing has revealed the following species in the S. schenckii complex: Sporothrix albicans, Sporothrix brasiliensis, Sporothrix globosa, Sporothrix luriei, Sporothrix mexicana and S. schenckii. The increasing number of reports of Sporothrix infection in immunocompromised patients, mainly the HIV-infected population, suggests sporotrichosis as an emerging global health problem concomitant with the AIDS pandemic. Molecular studies have demonstrated a high level of intraspecific variability. Components of the S. schenckii cell wall that act as adhesins and immunogenic inducers, such as a 70-kDa glycoprotein, are apparently specific to this fungus. The main glycan peptidorhamnomannan cell wall component is the only O-linked glycan structure known in S. schenckii. It contains an α-mannobiose c...
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Isolation and some properties of a glycoprotein of 70 kDa (Gp70) from the cell wall of Sporothrix schenckii involved in fungal adherence to dermal extracellular matrix
Medical mycology, 2008Co-Authors: Estela Ruiz-baca, Armando Pérez-torres, Julio C. Villagómez-castro, Conchita Toriello, Myrna Sabanero-lópez, Everardo López-romeroAbstract:Sporothrix schenckii is the etiological agent of sporotrichosis, a Subcutaneous Mycosis and an emerging disease in immunocompromised patients. Adherence to target cells is a prerequisite for fungal dissemination and systemic complications. However, information on the cell surface components involved in this interaction is rather scarce. In this investigation, the extraction of isolated cell walls from the yeast phase of S. schenckii with SDS and separation of proteins by SDS-PAGE led to the identification of a periodic acid-Schiff (PAS)-reacting 70 kDa glycoprotein (Gp70) that was purified by elution from electrophoresis gels. The purified glycopeptide exhibited a pI of 4.1 and about 5.7% of its molecular mass was contributed by N-linked glycans with no evidence for O-linked oligosaccharides. Confocal analysis of immunofluorescence assays with polyclonal antibodies directed towards Gp70 revealed a rather uniform distribution of the antigen at the cell surface with no distinguishable differences among thre...
