The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Gurpreet S Ahuja - One of the best experts on this subject based on the ideXlab platform.
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dynamic programming and automated segmentation of optical coherence tomography images of the neonatal Subglottis enabling efficient diagnostics to manage subglottic stenosis
Journal of Biomedical Optics, 2019Co-Authors: Konrad M Kozlowski, Jason J Chen, Gurpreet S Ahuja, Giriraj K Sharma, Joseph C Jing, Li Qi, Kathryn Osann, Andrew E Heidari, Philsang ChungAbstract:Subglottic stenosis (SGS) is a challenging disease to diagnose in neonates. Long-range optical coherence tomography (OCT) is an optical imaging modality that has been described to image the Subglottis in intubated neonates. A major challenge associated with OCT imaging is the lack of an automated method for image analysis and micrometry of large volumes of data that are acquired with each airway scan (1 to 2 Gb). We developed a tissue segmentation algorithm that identifies, measures, and conducts image analysis on tissue layers within the mucosa and submucosa and compared these automated tissue measurements with manual tracings. We noted small but statistically significant differences in thickness measurements of the mucosa and submucosa layers in the larynx ( p p = 0.015), and trachea ( p = 0.012). The automated algorithm was also shown to be over 8 times faster than the manual approach. Moderate Pearson correlations were found between different tissue texture parameters and the patient’s gestational age at birth, age in days, duration of intubation, and differences with age (mean age 17 days). Automated OCT data analysis is necessary in the diagnosis and monitoring of SGS, as it can provide vital information about the airway in real time and aid clinicians in making management decisions for intubated neonates.
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long range fourier domain optical coherence tomography of the pediatric Subglottis
International Journal of Pediatric Otorhinolaryngology, 2015Co-Authors: Veronika Volgger, Erica Su, Alex Wang, Zhongping Chen, Giriraj K Sharma, Joseph C Jing, Yasin Peaks, Frances Lazarow, Yueqiao Qu, Gurpreet S AhujaAbstract:Background Acquired subglottic stenosis (SGS) most commonly results from prolonged endotracheal intubation and is a diagnostic challenge in the intubated child. At present, no imaging modality allows for in vivo characterization of subglottic microanatomy to identify early signs of acquired SGS while the child remains intubated. Fourier domain optical coherence tomography (FD-OCT) is a minimally invasive, light-based imaging modality which provides high resolution, three dimensional (3D) cross-sectional images of biological tissue. We used long-range FD-OCT to image the Subglottis in intubated pediatric patients undergoing minor head and neck surgical procedures in the operating room.
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analysis and digital 3d modeling of long range fourier domain optical coherence tomography images of the pediatric Subglottis
Proceedings of SPIE, 2014Co-Authors: Erica Su, K Giriraj M D Sharma, Jason J Chen, Tony D Nguyen, Alex Wang, Ashley Hamamoto, Gurpreet S Ahuja, Zhongping Chen, M Brian Jf D WongAbstract:In neonatal and pediatric patients who require long-term endotracheal intubation, the subglottic mucosa is most susceptible to injury from the endotracheal tube. At present, there is no diagnostic modality to identify early signs of subglottic mucosal pathology. Fourier-domain optical coherence tomography (FD-OCT) is a minimally-invasive imaging modality which acquires high-resolution, 3D cross-sectional images of biological tissue. FD-OCT of the neonatal and pediatric airways was conducted to evaluate subglottic microanatomy and histopathologic changes associated with prolonged intubation. FD-OCT of the larynx, Subglottis and proximal trachea was conducted in pediatric and neonatal patients. OCT image sets were analyzed by anatomic categorization (airway level), tissue segmentation and mucosa micrometry in MATLAB. Subsequently, OCT data sets were rendered into digital 3D airway models in Mimics software. We report original methods for subglottic OCT image processing and analysis.
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characterization of submucosal lesions using optical coherence tomography in the rabbit Subglottis
Archives of Otolaryngology-head & Neck Surgery, 2005Co-Authors: Amir M Karamzadeh, Gurpreet S Ahuja, Zhongping Chen, Ryan S Jackson, James M Ridgway, Hau Sin Wong, Michael C Chao, Lihhuei L Liaw, Brian J F WongAbstract:Objective To evaluate the efficacy of optical coherence tomography in differentiating between several simulated subglottic lesions, using an ex vivo rabbit laryngotracheal model. Design Laryngotracheal complexes were harvested from euthanized rabbits and divided into the following 4 groups: (1) control, (2) submucosal collagen injection (simulating scar formation), (3) dehydration and rehydration (simulating edema), and (4) repeated intubation trauma. The subglottic region was imaged using optical coherence tomography. Images were later correlated with conventional histologic findings. Results The epithelium, basement membrane, lamina propria, perichondrium, and cartilage (cricoid and tracheal) were clearly imaged. In group 2, an increase in the thickness of the lamina propria was observed, in addition to a characteristic optical pattern of the injected collagen. Dehydration (in group 3) produced a visible reduction in the thickness of the lamina propria, while rehydration of the same specimen with distilled water revealed a significant increase in submucosal swelling. Repeated intubation (in group 4) resulted in tissue edema that was seen as wavy heterogeneous thickening of the lamina propria. Edema produced by repeated intubation or distilled water immersion was easily differentiated from native and collagen-injected tissues. Conclusion Optical coherence tomography successfully identifies the microstructure layers of the Subglottis and can differentiate between edema and increased collagen deposition in the rabbit model.
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distinguishing edema vs scar in the rabbit Subglottis using optical coherence tomography
Otolaryngology-Head and Neck Surgery, 2004Co-Authors: Amir M Karamzadeh, Gurpreet S Ahuja, Zhongping Chen, Ryan S Jackson, Michael C Chao, Brian J F WongAbstract:Abstract Problem: In neonates, subglottic stenosis is a tremendous problem related to prolonged enotracheal intubation. Despite the use of diagnostic endoscopy, differentiating between edema, scar, or neocartilage formation remains a challenge. Optical Coherence Tomography (OCT) is an imaging modality that uses light to produce high-resolution cross-sectional images in tissue. Using an ex vivo rabbit laryngotracheal model, we evaluate the efficacy of OCT to differentiate between several simulated subglottic pathologies. Methods: Seven laryngeal-tracheal complexes were harvested from freshly sacrificed rabbits. The specimens were divided into 4 groups: (1) control; (2) submucosal collagen injection (simulating scar formation); (3) dehydration/hydration (simulating edema); and (4) repeated intubation trauma. The subglottic region was imaged using an OCT system that produced images with axial and lateral resolution of 10 and 20 μm, respectively. The submucosal thickness (basement membrane to perichondrium) was measured in each group and compared to controls. OCT images were later correlated with conventional histology. Results: The epithelium, basement membrane, lamina propria, perichondrium, and cartilage (cricoid and tracheal) were very clearly imaged. A 50% increase in submucosal thickness was observed in group 2, and the collagen was clearly identified and distinct from surrounding native tissues. Dehydration (group 3) produced a 60% reduction while rehydrating the same specimen in distilled water resulted in a 350% increase. Edema, produced by either repeated intubation or distilled water immersion, was easily differentiated from both native and the collagen-injected tissues. Conclusion: OCT successfully identifies the layer microstructure of the Subglottis and can be used to differentiate between edema and increased collagen in the rabbit model. Significance: OCT can be readily adapted for use during rigid and flexible endoscopy. Likewise, since OCT imaging fibers can be placed within endotracheal tubes to image the airway, the technique is an extremely attractive option for use in the ICU to manage the neonatal airway. Support: None reported.
Brian J F Wong - One of the best experts on this subject based on the ideXlab platform.
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characterization of submucosal lesions using optical coherence tomography in the rabbit Subglottis
Archives of Otolaryngology-head & Neck Surgery, 2005Co-Authors: Amir M Karamzadeh, Gurpreet S Ahuja, Zhongping Chen, Ryan S Jackson, James M Ridgway, Hau Sin Wong, Michael C Chao, Lihhuei L Liaw, Brian J F WongAbstract:Objective To evaluate the efficacy of optical coherence tomography in differentiating between several simulated subglottic lesions, using an ex vivo rabbit laryngotracheal model. Design Laryngotracheal complexes were harvested from euthanized rabbits and divided into the following 4 groups: (1) control, (2) submucosal collagen injection (simulating scar formation), (3) dehydration and rehydration (simulating edema), and (4) repeated intubation trauma. The subglottic region was imaged using optical coherence tomography. Images were later correlated with conventional histologic findings. Results The epithelium, basement membrane, lamina propria, perichondrium, and cartilage (cricoid and tracheal) were clearly imaged. In group 2, an increase in the thickness of the lamina propria was observed, in addition to a characteristic optical pattern of the injected collagen. Dehydration (in group 3) produced a visible reduction in the thickness of the lamina propria, while rehydration of the same specimen with distilled water revealed a significant increase in submucosal swelling. Repeated intubation (in group 4) resulted in tissue edema that was seen as wavy heterogeneous thickening of the lamina propria. Edema produced by repeated intubation or distilled water immersion was easily differentiated from native and collagen-injected tissues. Conclusion Optical coherence tomography successfully identifies the microstructure layers of the Subglottis and can differentiate between edema and increased collagen deposition in the rabbit model.
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distinguishing edema vs scar in the rabbit Subglottis using optical coherence tomography
Otolaryngology-Head and Neck Surgery, 2004Co-Authors: Amir M Karamzadeh, Gurpreet S Ahuja, Zhongping Chen, Ryan S Jackson, Michael C Chao, Brian J F WongAbstract:Abstract Problem: In neonates, subglottic stenosis is a tremendous problem related to prolonged enotracheal intubation. Despite the use of diagnostic endoscopy, differentiating between edema, scar, or neocartilage formation remains a challenge. Optical Coherence Tomography (OCT) is an imaging modality that uses light to produce high-resolution cross-sectional images in tissue. Using an ex vivo rabbit laryngotracheal model, we evaluate the efficacy of OCT to differentiate between several simulated subglottic pathologies. Methods: Seven laryngeal-tracheal complexes were harvested from freshly sacrificed rabbits. The specimens were divided into 4 groups: (1) control; (2) submucosal collagen injection (simulating scar formation); (3) dehydration/hydration (simulating edema); and (4) repeated intubation trauma. The subglottic region was imaged using an OCT system that produced images with axial and lateral resolution of 10 and 20 μm, respectively. The submucosal thickness (basement membrane to perichondrium) was measured in each group and compared to controls. OCT images were later correlated with conventional histology. Results: The epithelium, basement membrane, lamina propria, perichondrium, and cartilage (cricoid and tracheal) were very clearly imaged. A 50% increase in submucosal thickness was observed in group 2, and the collagen was clearly identified and distinct from surrounding native tissues. Dehydration (group 3) produced a 60% reduction while rehydrating the same specimen in distilled water resulted in a 350% increase. Edema, produced by either repeated intubation or distilled water immersion, was easily differentiated from both native and the collagen-injected tissues. Conclusion: OCT successfully identifies the layer microstructure of the Subglottis and can be used to differentiate between edema and increased collagen in the rabbit model. Significance: OCT can be readily adapted for use during rigid and flexible endoscopy. Likewise, since OCT imaging fibers can be placed within endotracheal tubes to image the airway, the technique is an extremely attractive option for use in the ICU to manage the neonatal airway. Support: None reported.
D M Albert - One of the best experts on this subject based on the ideXlab platform.
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photometries a new method of measuring the cross sectional area of the Subglottis
Clinical Otolaryngology, 1992Co-Authors: N S Jones, R M Irving, G R Ford, A Balakrishnan, D M AlbertAbstract:: A new endoscopic method of measuring the cross-sectional area of a subglottic stenosis is presented. The method was tested against existing techniques using a model. By standardizing the distance from the objective of the endoscope to the stenotic area we were able to measure the cross-sectional area using a graduated grid held against the video screen. This technique was then compared with the standard one of estimating the diameter of a stenosis from the external diameter of the bronchoscope which can be passed through the stenosis. The methods were found to be equally accurate in measuring the lumen diameter. However, when measuring the minimum cross-sectional area, three of five observers were significantly better (P less than 0.01) using the photometric method rather than the standard method (Wilcoxon's rank sum test for paired data). The photometric technique of measuring the cross-sectional area of the Subglottis is easily performed, non-traumatic, and allows a visual record to be kept.
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Photometries: a new method of measuring the cross‐sectional area of the Subglottis
Clinical Otolaryngology, 1992Co-Authors: N S Jones, R M Irving, G R Ford, A Balakrishnan, D M AlbertAbstract:: A new endoscopic method of measuring the cross-sectional area of a subglottic stenosis is presented. The method was tested against existing techniques using a model. By standardizing the distance from the objective of the endoscope to the stenotic area we were able to measure the cross-sectional area using a graduated grid held against the video screen. This technique was then compared with the standard one of estimating the diameter of a stenosis from the external diameter of the bronchoscope which can be passed through the stenosis. The methods were found to be equally accurate in measuring the lumen diameter. However, when measuring the minimum cross-sectional area, three of five observers were significantly better (P less than 0.01) using the photometric method rather than the standard method (Wilcoxon's rank sum test for paired data). The photometric technique of measuring the cross-sectional area of the Subglottis is easily performed, non-traumatic, and allows a visual record to be kept.
Kiyofumi Mitsui - One of the best experts on this subject based on the ideXlab platform.
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transtracheal endoluminal resection of a pleomorphic adenoma occluding Subglottis
European Journal of Cardio-Thoracic Surgery, 2008Co-Authors: Shigemi Ishikawa, Masaki Kimura, Yukinori Inadome, Kiyofumi MitsuiAbstract:: A 71-year-old male was treated for suspected bronchial asthma because of dyspnea and stridor for 3 months before presenting at our hospital. Chest computed tomogram and a laryngotracheoscopy revealed a mass occupying the subglottic cavity. Instead of a laryngotracheal resection, the tumor was extirpated from the posterior wall of the Subglottis and the first two tracheal rings successfully through a vertical tracheotomy just above the life-saving trachestomy tube, and was diagnosed as pleomorphic adenoma. The patient is alive and well with no recurrent tumor 12 years after surgery, without any effect on the function of the voice or swallowing.
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case report transtracheal endoluminal resection of a pleomorphic adenoma occluding Subglottis
2008Co-Authors: Shigemi Ishikawa, Masaki Kimura, Yukinori Inadome, Kiyofumi MitsuiAbstract:A 71-year-old male was treated for suspected bronchial asthma because of dyspnea and stridor for 3 months before presenting at our hospital. Chest computed tomogram and a laryngotracheoscopy revealed a mass occupying the subglottic cavity. Instead of a laryngotracheal resection, the tumor was extirpated from the posterior wall of the Subglottis and thefirst two tracheal rings successfully through a vertical tracheotomy just above the life-saving trachestomy tube, and was diagnosed as pleomorphic adenoma. The patient is alive and well with no recurrent tumor 12 years after surgery, without any effect on the function of the voice or swallowing. # 2008 European Association for Cardio-Thoracic Surgery. Published by Elsevier B.V. All rights reserved.
Amir M Karamzadeh - One of the best experts on this subject based on the ideXlab platform.
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characterization of submucosal lesions using optical coherence tomography in the rabbit Subglottis
Archives of Otolaryngology-head & Neck Surgery, 2005Co-Authors: Amir M Karamzadeh, Gurpreet S Ahuja, Zhongping Chen, Ryan S Jackson, James M Ridgway, Hau Sin Wong, Michael C Chao, Lihhuei L Liaw, Brian J F WongAbstract:Objective To evaluate the efficacy of optical coherence tomography in differentiating between several simulated subglottic lesions, using an ex vivo rabbit laryngotracheal model. Design Laryngotracheal complexes were harvested from euthanized rabbits and divided into the following 4 groups: (1) control, (2) submucosal collagen injection (simulating scar formation), (3) dehydration and rehydration (simulating edema), and (4) repeated intubation trauma. The subglottic region was imaged using optical coherence tomography. Images were later correlated with conventional histologic findings. Results The epithelium, basement membrane, lamina propria, perichondrium, and cartilage (cricoid and tracheal) were clearly imaged. In group 2, an increase in the thickness of the lamina propria was observed, in addition to a characteristic optical pattern of the injected collagen. Dehydration (in group 3) produced a visible reduction in the thickness of the lamina propria, while rehydration of the same specimen with distilled water revealed a significant increase in submucosal swelling. Repeated intubation (in group 4) resulted in tissue edema that was seen as wavy heterogeneous thickening of the lamina propria. Edema produced by repeated intubation or distilled water immersion was easily differentiated from native and collagen-injected tissues. Conclusion Optical coherence tomography successfully identifies the microstructure layers of the Subglottis and can differentiate between edema and increased collagen deposition in the rabbit model.
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distinguishing edema vs scar in the rabbit Subglottis using optical coherence tomography
Otolaryngology-Head and Neck Surgery, 2004Co-Authors: Amir M Karamzadeh, Gurpreet S Ahuja, Zhongping Chen, Ryan S Jackson, Michael C Chao, Brian J F WongAbstract:Abstract Problem: In neonates, subglottic stenosis is a tremendous problem related to prolonged enotracheal intubation. Despite the use of diagnostic endoscopy, differentiating between edema, scar, or neocartilage formation remains a challenge. Optical Coherence Tomography (OCT) is an imaging modality that uses light to produce high-resolution cross-sectional images in tissue. Using an ex vivo rabbit laryngotracheal model, we evaluate the efficacy of OCT to differentiate between several simulated subglottic pathologies. Methods: Seven laryngeal-tracheal complexes were harvested from freshly sacrificed rabbits. The specimens were divided into 4 groups: (1) control; (2) submucosal collagen injection (simulating scar formation); (3) dehydration/hydration (simulating edema); and (4) repeated intubation trauma. The subglottic region was imaged using an OCT system that produced images with axial and lateral resolution of 10 and 20 μm, respectively. The submucosal thickness (basement membrane to perichondrium) was measured in each group and compared to controls. OCT images were later correlated with conventional histology. Results: The epithelium, basement membrane, lamina propria, perichondrium, and cartilage (cricoid and tracheal) were very clearly imaged. A 50% increase in submucosal thickness was observed in group 2, and the collagen was clearly identified and distinct from surrounding native tissues. Dehydration (group 3) produced a 60% reduction while rehydrating the same specimen in distilled water resulted in a 350% increase. Edema, produced by either repeated intubation or distilled water immersion, was easily differentiated from both native and the collagen-injected tissues. Conclusion: OCT successfully identifies the layer microstructure of the Subglottis and can be used to differentiate between edema and increased collagen in the rabbit model. Significance: OCT can be readily adapted for use during rigid and flexible endoscopy. Likewise, since OCT imaging fibers can be placed within endotracheal tubes to image the airway, the technique is an extremely attractive option for use in the ICU to manage the neonatal airway. Support: None reported.
