The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform
Sante Capasso - One of the best experts on this subject based on the ideXlab platform.
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Effect of lysine residues on the deamidation reaction of asparagine side chains.
Biopolymers, 2000Co-Authors: Sante Capasso, Gianfranco Balboni, Paola Di CerboAbstract:The effect of lysine residues on the deamidation reaction of the asparagine side chain has been studied on the peptide and on its lysine-acetylated Derivative in a wide range of pH values. The amino acid sequence of these peptides is similar to the local sequence flanking the labile Asn-67 in RNAse A. The experimental data show that Lys influences both the deamidation rate and the relative yield of the two reaction products, i.e., the aspartic acid and β-aspartic acid containing peptide. These effects are pH dependent and can be rationalized based on the mechanism previously proposed for the deamidation reaction via Succinimide Derivative. © 2000 John Wiley & Sons, Inc. Biopoly 53: 213–219, 2000
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thermodynamic parameters of the reversible isomerization of aspartic residues via a Succinimide Derivative
Thermochimica Acta, 1996Co-Authors: Sante CapassoAbstract:Abstract Values of the thermodynamic quantities ΔH ° and ΔS ° for the reactions “Asp-peptide ⇆ Asupeptide ⇆ β-Asp-peptide” in aqueous solution have been obtained for the model peptides AcGlyXGlyGlyNHMe and AcXGlyNHMe (X Asp, β-Asp, Asu; Asu = aminosuccinyl residue) from the temperature-dependence of equilibrium constants. The ΔH ° and ΔS ° values for the cyclization reactions of the carboxylic-acid form of the Asp and β-Asp side chains of the dipeptides and tetrapeptides are positive and coincident within experimental error. Medium values are 34 kJ mol −1 and 127 J K −1 mol −1 for ΔH ° and ΔS °, respectively. The molar enthalpies and molar entropies of the Asp-dipeptide and β-Asp-dipeptide, and of the Asp-tetrapeptide and β-Asp-tetrapeptide, did not exhibit significant differences.
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Kinetics and mechanism of Succinimide ring formation in the deamidation process of asparagine residues
Journal of The Chemical Society-perkin Transactions 1, 1993Co-Authors: Sante Capasso, Lelio Mazzarella, Adriana Zagari, Filomena Sica, Severo SalvadoriAbstract:The cyclization of Ac–Gly–Asn–Gly–Gly–NHMe to the Succinimide Derivative has been studied in the pH range 5.5–10.4 at 37° and µ= 1 mol dm–3. Kinetic evidence indicates that the reaction is a multistep process with a change in the rate-determining step at pH 6.5–7.0.The suggested mechanism involves the pre-equilibrium deprotonation of the NH group next to the Asn residue, followed by nucleophilic attack of the nitrogen atom on the carbonyl carbon of the Asn side chain giving a cyclic tetrahedral intermediate. At acidic pH the cyclization step is rate determining, whereas, the removal of the leaving group by apparent general-base catalysis is the rate-determining step at neutral and basic pH. The literature data on the deamidation rate are discussed in light of the proposed mechanism.
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spontaneous cyclization of the aspartic acid side chain to the Succinimide Derivative
Journal of The Chemical Society Chemical Communications, 1992Co-Authors: Sante Capasso, Lelio Mazzarella, F Sica, Adriana Zagari, Severo SalvadoriAbstract:At equilibrium in acidic solution the Succinimide Derivative, formed by cyclization reaction of the Asp side chain, predominates; at a pH close to the apparent pKa of the Asp residue this reaction is relatively fast with a t1/2 of a few days.
Ewa Marzęda - One of the best experts on this subject based on the ideXlab platform.
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Effect of N-(p-ethoxycarbonylphenylmethyl) - p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Journal of Pre-Clinical and Clinical Research, 2014Co-Authors: Ewa Marzęda, Maria W. Kondrat-wróbel, Sergey L. Kocharov, Jarogniew J. Łuszczki, Jan Wróbel, Magdalena Florek-ŁuszczkiAbstract:Introduction and objective. The purpose of this study was to determine the effects of N-(p-ethoxycarbonylphenylmethyl)p-isopropoxyphenylSuccinimide (ECPM-IPPS), a new Succinimide Derivative, on the protective action of four classical antiepileptic drugs (AEDs): carbamazepine (CBZ), phenobarbital (PB), phenytoin (PHT) and valproate (VPA) in the mouse maximal electroshock (MES)-induced tonic seizure model. Materials and methods. Tonic hind limb extension (seizure activity) was evoked in adult male albino Swiss mice by a current (sine-wave, 25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via ear-clip electrodes. Results. ECPM-IPPS administered (i.p.) at a dose of 150 mg/kg significantly elevated the threshold for electroconvulsions in mice (P
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Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Current Issues in Pharmacy and Medical Sciences, 2014Co-Authors: Jarogniew J. Luszczki, Ewa Marzęda, Maria W. Kondrat-wróbel, Daniel Pyrka, Sergey L. Kocharov, Magdalena Florek-luszczkiAbstract:Experimental evidence indicates that several Succinimide Derivatives possess anticonvulsant properties in animal models of epilepsy [1,2,5-11,14,15]. In our pilot study, we found that N-(m-bromoanilinomethyl)-p-isopropoxyphenyl-Succinimide (BAM-IPPS; Fig. 1. – a new Succinimide Derivative) exerted the anticonvulsant properties by suppressing tonic-clonic seizures in the mouse maximal electroshock-induced seizure (MES) test (unpublished data). Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Magdalena Florek-luszczki - One of the best experts on this subject based on the ideXlab platform.
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Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Current Issues in Pharmacy and Medical Sciences, 2014Co-Authors: Jarogniew J. Luszczki, Ewa Marzęda, Maria W. Kondrat-wróbel, Daniel Pyrka, Sergey L. Kocharov, Magdalena Florek-luszczkiAbstract:Experimental evidence indicates that several Succinimide Derivatives possess anticonvulsant properties in animal models of epilepsy [1,2,5-11,14,15]. In our pilot study, we found that N-(m-bromoanilinomethyl)-p-isopropoxyphenyl-Succinimide (BAM-IPPS; Fig. 1. – a new Succinimide Derivative) exerted the anticonvulsant properties by suppressing tonic-clonic seizures in the mouse maximal electroshock-induced seizure (MES) test (unpublished data). Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Sergey L. Kocharov - One of the best experts on this subject based on the ideXlab platform.
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Effect of N-(p-ethoxycarbonylphenylmethyl) - p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Journal of Pre-Clinical and Clinical Research, 2014Co-Authors: Ewa Marzęda, Maria W. Kondrat-wróbel, Sergey L. Kocharov, Jarogniew J. Łuszczki, Jan Wróbel, Magdalena Florek-ŁuszczkiAbstract:Introduction and objective. The purpose of this study was to determine the effects of N-(p-ethoxycarbonylphenylmethyl)p-isopropoxyphenylSuccinimide (ECPM-IPPS), a new Succinimide Derivative, on the protective action of four classical antiepileptic drugs (AEDs): carbamazepine (CBZ), phenobarbital (PB), phenytoin (PHT) and valproate (VPA) in the mouse maximal electroshock (MES)-induced tonic seizure model. Materials and methods. Tonic hind limb extension (seizure activity) was evoked in adult male albino Swiss mice by a current (sine-wave, 25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via ear-clip electrodes. Results. ECPM-IPPS administered (i.p.) at a dose of 150 mg/kg significantly elevated the threshold for electroconvulsions in mice (P
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Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Current Issues in Pharmacy and Medical Sciences, 2014Co-Authors: Jarogniew J. Luszczki, Ewa Marzęda, Maria W. Kondrat-wróbel, Daniel Pyrka, Sergey L. Kocharov, Magdalena Florek-luszczkiAbstract:Experimental evidence indicates that several Succinimide Derivatives possess anticonvulsant properties in animal models of epilepsy [1,2,5-11,14,15]. In our pilot study, we found that N-(m-bromoanilinomethyl)-p-isopropoxyphenyl-Succinimide (BAM-IPPS; Fig. 1. – a new Succinimide Derivative) exerted the anticonvulsant properties by suppressing tonic-clonic seizures in the mouse maximal electroshock-induced seizure (MES) test (unpublished data). Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Maria W. Kondrat-wróbel - One of the best experts on this subject based on the ideXlab platform.
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Effect of N-(p-ethoxycarbonylphenylmethyl) - p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Journal of Pre-Clinical and Clinical Research, 2014Co-Authors: Ewa Marzęda, Maria W. Kondrat-wróbel, Sergey L. Kocharov, Jarogniew J. Łuszczki, Jan Wróbel, Magdalena Florek-ŁuszczkiAbstract:Introduction and objective. The purpose of this study was to determine the effects of N-(p-ethoxycarbonylphenylmethyl)p-isopropoxyphenylSuccinimide (ECPM-IPPS), a new Succinimide Derivative, on the protective action of four classical antiepileptic drugs (AEDs): carbamazepine (CBZ), phenobarbital (PB), phenytoin (PHT) and valproate (VPA) in the mouse maximal electroshock (MES)-induced tonic seizure model. Materials and methods. Tonic hind limb extension (seizure activity) was evoked in adult male albino Swiss mice by a current (sine-wave, 25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via ear-clip electrodes. Results. ECPM-IPPS administered (i.p.) at a dose of 150 mg/kg significantly elevated the threshold for electroconvulsions in mice (P
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Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
Current Issues in Pharmacy and Medical Sciences, 2014Co-Authors: Jarogniew J. Luszczki, Ewa Marzęda, Maria W. Kondrat-wróbel, Daniel Pyrka, Sergey L. Kocharov, Magdalena Florek-luszczkiAbstract:Experimental evidence indicates that several Succinimide Derivatives possess anticonvulsant properties in animal models of epilepsy [1,2,5-11,14,15]. In our pilot study, we found that N-(m-bromoanilinomethyl)-p-isopropoxyphenyl-Succinimide (BAM-IPPS; Fig. 1. – a new Succinimide Derivative) exerted the anticonvulsant properties by suppressing tonic-clonic seizures in the mouse maximal electroshock-induced seizure (MES) test (unpublished data). Effect of N-(m-bromoanilinomethyl)-p-isopropoxyphenylSuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
