The Experts below are selected from a list of 3582 Experts worldwide ranked by ideXlab platform
Huizhong Wang - One of the best experts on this subject based on the ideXlab platform.
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Succinyl-proteome profiling of Dendrobium officinale , an important traditional Chinese orchid herb, revealed involvement of Succinylation in the glycolysis pathway
BMC genomics, 2017Co-Authors: Shangguo Feng, Kaili Jiao, Hong Guo, Mengyi Jiang, Juan Hao, Huizhong Wang, Chenjia ShenAbstract:Lysine Succinylation is a ubiquitous and important protein post-translational modification in various eukaryotic and prokaryotic cells. However, its functions in Dendrobium officinale, an important traditional Chinese orchid herb with high polysaccharide contents, are largely unknown. In our study, LC-MS/MS was used to identify the peptides that were enriched by immune-purification with a high-efficiency succinyl-lysine antibody. In total, 314 lysine Succinylation sites in 207 proteins were identified. A gene ontology analysis showed that these proteins are associated with a wide range of cellular functions, from metabolic processes to stimuli responses. Moreover, two types of conserved Succinylation motifs, ‘***Ksuc******K**’ and ‘****EKsuc***’, were identified. Our data showed that lysine Succinylation occurred on five key enzymes in the glycolysis pathway. The numbers of average Succinylation sites on these five enzymes in plants were lower than those in bacteria and mammals. Interestingly, two active site amino acids residues, K103 and K225, could be succinylated in fructose-bisphosphate aldolase, indicating a potential function of lysine Succinylation in the regulation of glycolytic enzyme activities. Furthermore, the protein–protein interaction network for the succinylated proteins showed that several functional terms, such as glycolysis, TCA cycle, oxidative phosphorylation and ribosome, are consisted. Our results provide the first comprehensive view of the succinylome of D. officinale and may accelerate future biological investigations of Succinylation in the synthesis of polysaccharides, which are major active ingredients.
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Succinyl-proteome profiling of a high taxol containing hybrid Taxus species (Taxus × media) revealed involvement of Succinylation in multiple metabolic pathways.
Scientific reports, 2016Co-Authors: Chenjia Shen, Hong Guo, Jie Xue, Tao Sun, Lei Zhang, Yijun Meng, Huizhong WangAbstract:Protein lysine Succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 Succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine Succinylation in Taxus × media involved a diverse array of metabolic processes and protein–protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine Succinylation, suggesting the presence of a common mechanism underlying the participation of Succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of Succinylation.
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succinyl proteome profiling of a high taxol containing hybrid taxus species taxus media revealed involvement of Succinylation in multiple metabolic pathways
Scientific Reports, 2016Co-Authors: Chenjia Shen, Hong Guo, Jie Xue, Tao Sun, Lei Zhang, Yijun Meng, Huizhong WangAbstract:Protein lysine Succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 Succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine Succinylation in Taxus × media involved a diverse array of metabolic processes and protein–protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine Succinylation, suggesting the presence of a common mechanism underlying the participation of Succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of Succinylation.
Chenjia Shen - One of the best experts on this subject based on the ideXlab platform.
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Quantitative succinyl-proteome profiling of Chinese hickory ( Carya cathayensis ) during the grafting process
BMC plant biology, 2019Co-Authors: Hu Wei Yuan, Dao Liang Yan, Juanjuan Chen, Ying Yang, Chenjia Shen, Junfeng Wang, Bing Song ZhengAbstract:Chinese hickory (Carya cathayensis) is a popular nut plant having high economic value. Grafting is applied to accelerate the transition from vegetative phase to reproductive phase. Lysine Succinylation occurs frequently in the proteins associated with metabolic pathways, which may participate in the regulation of the grafting process. However, the exact regulatory mechanism underlying grafting process in Chinese hickory has not been studied at post-translational modification level. A comprehensive proteome-wide lysine Succinylation profiling of Chinese hickory was explored by a newly developed method combining affinity enrichment and high-resolution LC-MS/MS. In total, 259 Succinylation sites in 202 proteins were identified, representing the first comprehensive lysine succinylome in Chinese hickory. The Succinylation was biased to occur in the cytosolic proteins of Chinese hickory. Moreover, four conserved Succinylation motifs were identified in the succinylated peptides. Comparison of two grafting stages of Chinese hickory revealed that the differential expressed succinylated proteins were mainly involved in sugar metabolism, carbon fixation, amino acid metabolism and plant-pathogen interaction. Besides, seven heat shock proteins (HSPs) with 11 Succinylation sites were also identified, all of which were observed to be up-regulated during the grafting process. Succinylation of the proteins involved in amino acid biosynthesis might be required for a successful grafting. Succinylated HSPs might play a role in stress tolerance of the grafted Chinese hickory plants. Our results can be a good resource for functional validation of the succinylated proteins and a starting point for the investigation of molecular mechanisms during lysine Succinylation occurring at grafting site.
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Succinyl-proteome profiling of Dendrobium officinale , an important traditional Chinese orchid herb, revealed involvement of Succinylation in the glycolysis pathway
BMC genomics, 2017Co-Authors: Shangguo Feng, Kaili Jiao, Hong Guo, Mengyi Jiang, Juan Hao, Huizhong Wang, Chenjia ShenAbstract:Lysine Succinylation is a ubiquitous and important protein post-translational modification in various eukaryotic and prokaryotic cells. However, its functions in Dendrobium officinale, an important traditional Chinese orchid herb with high polysaccharide contents, are largely unknown. In our study, LC-MS/MS was used to identify the peptides that were enriched by immune-purification with a high-efficiency succinyl-lysine antibody. In total, 314 lysine Succinylation sites in 207 proteins were identified. A gene ontology analysis showed that these proteins are associated with a wide range of cellular functions, from metabolic processes to stimuli responses. Moreover, two types of conserved Succinylation motifs, ‘***Ksuc******K**’ and ‘****EKsuc***’, were identified. Our data showed that lysine Succinylation occurred on five key enzymes in the glycolysis pathway. The numbers of average Succinylation sites on these five enzymes in plants were lower than those in bacteria and mammals. Interestingly, two active site amino acids residues, K103 and K225, could be succinylated in fructose-bisphosphate aldolase, indicating a potential function of lysine Succinylation in the regulation of glycolytic enzyme activities. Furthermore, the protein–protein interaction network for the succinylated proteins showed that several functional terms, such as glycolysis, TCA cycle, oxidative phosphorylation and ribosome, are consisted. Our results provide the first comprehensive view of the succinylome of D. officinale and may accelerate future biological investigations of Succinylation in the synthesis of polysaccharides, which are major active ingredients.
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Succinyl-proteome profiling of a high taxol containing hybrid Taxus species (Taxus × media) revealed involvement of Succinylation in multiple metabolic pathways.
Scientific reports, 2016Co-Authors: Chenjia Shen, Hong Guo, Jie Xue, Tao Sun, Lei Zhang, Yijun Meng, Huizhong WangAbstract:Protein lysine Succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 Succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine Succinylation in Taxus × media involved a diverse array of metabolic processes and protein–protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine Succinylation, suggesting the presence of a common mechanism underlying the participation of Succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of Succinylation.
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succinyl proteome profiling of a high taxol containing hybrid taxus species taxus media revealed involvement of Succinylation in multiple metabolic pathways
Scientific Reports, 2016Co-Authors: Chenjia Shen, Hong Guo, Jie Xue, Tao Sun, Lei Zhang, Yijun Meng, Huizhong WangAbstract:Protein lysine Succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 Succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine Succinylation in Taxus × media involved a diverse array of metabolic processes and protein–protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine Succinylation, suggesting the presence of a common mechanism underlying the participation of Succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of Succinylation.
Lei Zhang - One of the best experts on this subject based on the ideXlab platform.
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Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata
Journal of agricultural and food chemistry, 2018Co-Authors: Xianping Fang, Ya Xin, Zheliang Sheng, Hui Liu, Aili Jiang, Fang Wang, Qian Zha, Yang Jian, Lei ZhangAbstract:The various post-translational modifications of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that Succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in Succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that Succinylation is involved in the recognition of pollen-stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport pr...
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Succinyl-proteome profiling of a high taxol containing hybrid Taxus species (Taxus × media) revealed involvement of Succinylation in multiple metabolic pathways.
Scientific reports, 2016Co-Authors: Chenjia Shen, Hong Guo, Jie Xue, Tao Sun, Lei Zhang, Yijun Meng, Huizhong WangAbstract:Protein lysine Succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 Succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine Succinylation in Taxus × media involved a diverse array of metabolic processes and protein–protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine Succinylation, suggesting the presence of a common mechanism underlying the participation of Succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of Succinylation.
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succinyl proteome profiling of a high taxol containing hybrid taxus species taxus media revealed involvement of Succinylation in multiple metabolic pathways
Scientific Reports, 2016Co-Authors: Chenjia Shen, Hong Guo, Jie Xue, Tao Sun, Lei Zhang, Yijun Meng, Huizhong WangAbstract:Protein lysine Succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 Succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine Succinylation in Taxus × media involved a diverse array of metabolic processes and protein–protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine Succinylation, suggesting the presence of a common mechanism underlying the participation of Succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of Succinylation.
Hong Guo - One of the best experts on this subject based on the ideXlab platform.
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Succinyl-proteome profiling of Dendrobium officinale , an important traditional Chinese orchid herb, revealed involvement of Succinylation in the glycolysis pathway
BMC genomics, 2017Co-Authors: Shangguo Feng, Kaili Jiao, Hong Guo, Mengyi Jiang, Juan Hao, Huizhong Wang, Chenjia ShenAbstract:Lysine Succinylation is a ubiquitous and important protein post-translational modification in various eukaryotic and prokaryotic cells. However, its functions in Dendrobium officinale, an important traditional Chinese orchid herb with high polysaccharide contents, are largely unknown. In our study, LC-MS/MS was used to identify the peptides that were enriched by immune-purification with a high-efficiency succinyl-lysine antibody. In total, 314 lysine Succinylation sites in 207 proteins were identified. A gene ontology analysis showed that these proteins are associated with a wide range of cellular functions, from metabolic processes to stimuli responses. Moreover, two types of conserved Succinylation motifs, ‘***Ksuc******K**’ and ‘****EKsuc***’, were identified. Our data showed that lysine Succinylation occurred on five key enzymes in the glycolysis pathway. The numbers of average Succinylation sites on these five enzymes in plants were lower than those in bacteria and mammals. Interestingly, two active site amino acids residues, K103 and K225, could be succinylated in fructose-bisphosphate aldolase, indicating a potential function of lysine Succinylation in the regulation of glycolytic enzyme activities. Furthermore, the protein–protein interaction network for the succinylated proteins showed that several functional terms, such as glycolysis, TCA cycle, oxidative phosphorylation and ribosome, are consisted. Our results provide the first comprehensive view of the succinylome of D. officinale and may accelerate future biological investigations of Succinylation in the synthesis of polysaccharides, which are major active ingredients.
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Succinyl-proteome profiling of a high taxol containing hybrid Taxus species (Taxus × media) revealed involvement of Succinylation in multiple metabolic pathways.
Scientific reports, 2016Co-Authors: Chenjia Shen, Hong Guo, Jie Xue, Tao Sun, Lei Zhang, Yijun Meng, Huizhong WangAbstract:Protein lysine Succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 Succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine Succinylation in Taxus × media involved a diverse array of metabolic processes and protein–protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine Succinylation, suggesting the presence of a common mechanism underlying the participation of Succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of Succinylation.
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succinyl proteome profiling of a high taxol containing hybrid taxus species taxus media revealed involvement of Succinylation in multiple metabolic pathways
Scientific Reports, 2016Co-Authors: Chenjia Shen, Hong Guo, Jie Xue, Tao Sun, Lei Zhang, Yijun Meng, Huizhong WangAbstract:Protein lysine Succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 Succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine Succinylation in Taxus × media involved a diverse array of metabolic processes and protein–protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine Succinylation, suggesting the presence of a common mechanism underlying the participation of Succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of Succinylation.
Min Yang - One of the best experts on this subject based on the ideXlab platform.
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influence of Succinylation on physicochemical property of yak casein micelles
Food Chemistry, 2016Co-Authors: Min Yang, Jitao Yang, Yuan Zhang, Weibing ZhangAbstract:Abstract Succinylation is a chemical-modification method that affects the physicochemical characteristics and functional properties of proteins. This study assessed the influence of Succinylation on the physicochemical properties of yak casein micelles. The results revealed that surface hydrophobicity indices decreased with Succinylation. Additionally, denaturation temperature and denaturation enthalpy decreased with increasing Succinylation level, except at 82%. The buffering properties of yak casein micelles were affected by Succinylation. It was found that chemical modification contributed to a slight shift of the buffering peak towards a lower pH value and a markedly increase of the maximum buffering values of yak casein micelles at pH 4.5–6.0 and pH
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Influence of Succinylation on the conformation of yak casein micelles
Food chemistry, 2015Co-Authors: Min Yang, Jitao Yang, Na Cui, Yan Fang, Ying Shi, Jiangyu WangAbstract:Succinylation modifies the physicochemical characteristics and improves the functional properties of proteins. This study assessed the effects of Succinylation on the conformation of yak casein micelles with seven degree of modification. The results revealed that Succinylation contributed to the dissociation of casein micelles. With the increase of succinylated degree, soluble nitrogen and minerals content increased, while casein micelle size and polydispersity index of micelles decreased. Succinylation affected the spatial conformation of yak casein micelles: turn decreased, s-sheet and α-helix increased, and irregular structure were non-significantly affected. The intrinsic and ANS fluorescence intensity decreased and the maximum emission wavelength shifted red with increasing Succinylation. Based on the results, the structure of yak casein micelles was characteristic of the sub-micelle model.
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Effect of Succinylation on the functional properties of yak caseins: a comparison with cow caseins
Dairy Science & Technology, 2014Co-Authors: Min Yang, Ying Shi, Pengjie Wang, Hongna Liu, Pengcheng Wen, Fazheng RenAbstract:Yak caseins are special materials which has attracted more and more attention of researchers due to its increasing. Succinylation is a useful method of improving the functional properties of protein significantly. This study assessed the effects of Succinylation on the functional properties of yak and cow caseins, including solubility, emulsifying property, water and oil absorption capacity, foaming property, and digestibility. Six succinylated levels of caseins were prepared by adding different amounts of succinic anhydride to caseins. The succinylated degree of yak and cow caseins was different when similar amounts of succinic anhydride were added. Succinylation improved solubility, emulsifying activity, water and oil absorption capacity, and reduced foam stability and digestibility of yak and cow native caseins. However, Succinylation decreased foaming capacity and emulsion stability of cow caseins while increased them of yak caseins. The functional properties of caseins changed irregularly with increasing levels of Succinylation. The findings of this study revealed that Succinylation had different effects on the functional properties of different sources of caseins. The results will provide the basic data of functional properties of yak native caseins and succinylated yak caseins.
