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Dantong Liu - One of the best experts on this subject based on the ideXlab platform.
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Sucrose Metabolism in developing oil rich tubers of cyperus esculentus comparative transcriptome analysis
BMC Plant Biology, 2018Co-Authors: Zhenle Yang, Dantong LiuAbstract:Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots. Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc Metabolism in underground oil-rich storage tissues. In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc Metabolism. The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc Metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc Metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development. The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway. We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue. The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc Metabolism occurred in oil tubers of C. esculentus.
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Sucrose Metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis
BMC, 2018Co-Authors: Zhenle Yang, Dantong LiuAbstract:Abstract Background Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots. Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc Metabolism in underground oil-rich storage tissues. In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc Metabolism. Results The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc Metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc Metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development. The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway. Conclusions We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue. The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc Metabolism occurred in oil tubers of C. esculentus
Zhiguo Zhou - One of the best experts on this subject based on the ideXlab platform.
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Effects of Soil Salinity on Sucrose Metabolism in Cotton Leaves
PloS one, 2016Co-Authors: Jun Peng, Binglin Chen, Jingran Liu, Lei Zhang, Jun-yu Luo, Helin Dong, Xinhua Zhao, Ning Sui, Zhiguo ZhouAbstract:Cotton (Gosspium hirsutum L.) is classified as a salt tolerant crop. However, its yield and fiber quality are negatively affected by soil salinity. Studies on the enzymatic differences in Sucrose Metabolism under different soil salinity levels are lacking. Therefore, field experiments, using two cotton cultivars, CCRI-79 (salt-tolerant) and Simian 3 (salt-sensitive), were conducted in 2013 and 2014 at three different salinity levels (1.15 dS m-1 [low soil salinity], 6.00 dS m-1 [medium soil salinity], and 11.46 dS m-1 [high soil salinity]). The objective was to elucidate the effects of soil salinity on Sucrose content and the activity of key enzymes that are related to Sucrose Metabolism in cotton fiber. Results showed that as the soil salinity increased, cellulose content, Sucrose content, and Sucrose transformation rate declined; the decreases in cellulose content and Sucrose transformation rate caused by the increase in soil salinity were more in Simian 3 than those in CCRI-79. With increase in soil salinity, activities of Sucrose Metabolism enzymes Sucrose phophate synthase (SPS), acidic invertase, and alkaline invertase were decreased, whereas Sucrose synthase (SuSy) activity increased. However, the changes displayed in the SuSy and SPS activities in response to increase in soil salinity were different and the differences were large between the two cotton cultivars. These results illustrated that suppressed cellulose synthesis and Sucrose Metabolism under high soil salinity were mainly due to the change in SPS, SuSy, and invertase activities, and the difference in cellulose synthesis and Sucrose Metabolism in fiber for the two cotton cultivars in response to soil salinity was determined mainly by both SuSy and SPS activities.
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effects of soil salinity on Sucrose Metabolism in cotton fiber
PLOS ONE, 2016Co-Authors: Jun Peng, Zhiguo Zhou, Jingran Liu, Lei Zhang, Jun-yu Luo, Helin Dong, Xinhua Zhao, Ning Sui, Yali MengAbstract:Cotton (Gosspium hirsutum L.) is classified as a salt tolerant crop. However, its yield and fiber quality are negatively affected by soil salinity. Studies on the enzymatic differences in Sucrose Metabolism under different soil salinity levels are lacking. Therefore, field experiments, using two cotton cultivars, CCRI-79 (salt-tolerant) and Simian 3 (salt-sensitive), were conducted in 2013 and 2014 at three different salinity levels (1.15 dS m-1 [low soil salinity], 6.00 dS m-1 [medium soil salinity], and 11.46 dS m-1 [high soil salinity]). The objective was to elucidate the effects of soil salinity on Sucrose content and the activity of key enzymes that are related to Sucrose Metabolism in cotton fiber. Results showed that as the soil salinity increased, cellulose content, Sucrose content, and Sucrose transformation rate declined; the decreases in cellulose content and Sucrose transformation rate caused by the increase in soil salinity were more in Simian 3 than those in CCRI-79. With increase in soil salinity, activities of Sucrose Metabolism enzymes Sucrose phophate synthase (SPS), acidic invertase, and alkaline invertase were decreased, whereas Sucrose synthase (SuSy) activity increased. However, the changes displayed in the SuSy and SPS activities in response to increase in soil salinity were different and the differences were large between the two cotton cultivars. These results illustrated that suppressed cellulose synthesis and Sucrose Metabolism under high soil salinity were mainly due to the change in SPS, SuSy, and invertase activities, and the difference in cellulose synthesis and Sucrose Metabolism in fiber for the two cotton cultivars in response to soil salinity was determined mainly by both SuSy and SPS activities.
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effects of elevated temperature on Sucrose Metabolism and cellulose synthesis in cotton fibre during secondary cell wall development
Functional Plant Biology, 2015Co-Authors: Binglin Chen, Derrick M Oosterhuis, Wenqing Zhao, Yali Meng, Zhiguo Zhou, Youhua WangAbstract:Global warming has the potential to increase air temperatures by 1.8 to 4.0°C by the end of the 21st century. In order to reveal the effects of increased temperatures on the Sucrose Metabolism and cellulose synthesis in cotton fibre during its flowering and boll formation stage, field experiments with elevated temperature regimes (32.6/28.6°C, mean daytime/night-time temperature during flowering and boll formation stage during 2010–12, the same below) and ambient temperature regimes (30.1/25.8°C) were conducted. Activities of Sucrose synthase and acid/alkaline invertase decreased under elevated temperature in fibre, but activities of Sucrose phosphate synthase were increased. Callose content increased, but Sucrose content decreased within the cotton fibre under elevated temperature. The disparity of callose content and Sucrose content between the two temperature regimes decreased with the number of days post anthesis, indicating that the effects of elevated temperature on both Sucrose content and cellulose content were diminished as the boll matured. Due to the dynamics of the carbohydrate content and associated enzyme activities, we hypothesise that the restrained Sucrose Metabolism and cellulose biosynthesis under elevated temperatures were mainly attributed to the changed activities of Sucrose synthase and invertase. Furthermore, 32.6/28.6°C had a negative effect on the cellulose synthesis compared with 30.1/25.8°C.
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waterlogging during flowering and boll forming stages affects Sucrose Metabolism in the leaves subtending the cotton boll and its relationship with boll weight
Plant Science, 2014Co-Authors: Jie Kuai, Wenqing Zhao, Yali Meng, Binglin Chen, Youhua Wang, Zhiguo Zhou, Zhaowei Liu, Derrick M OosterhuisAbstract:The work explored Sucrose Metabolism in the leaves subtending the cotton boll (SBL) and its role in boll weight after waterlogging in cotton. Results showed that net photosynthesis rate (Pn), relative water content, contents of Chlorophyll a and Chlorophyll b, initial ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) activity and cytosolic fructose-1, 6-bisphosphatase (cy-FBPase) activity decreased with waterlogging in the SBL on fruiting branches 2-3 (FB2-3) and FB6-7. Activities of Sucrose synthase (SuSy) and Sucrose phosphate synthase (SPS) increased to the maximum up to 6 days of waterlogging then decreased with prolonged waterlogging. Rubisco activation and specific leaf weight increased and gene expressions of SuSy, SPS and rubisco activase (RCA) were all up-regulated with the duration of waterlogging, especially for the SBL on FB6-7. The induction of activity and gene expression of SuSy was most significant indicating its crucial role in Sucrose Metabolism after waterlogging. For the SBL in the later period of boll development on upper FB10-11 and FB14-15, the pattern seemed opposite to that of FB2-3 and FB6c7 as compensation effect in vegetative growth existed. Correlation analysis revealed that initial Rubisco activity and cy-FBPase activity were the main limitation to Pn reduction after waterlogging. Reduction in Pn, Sucrose transformation rate and initial Rubisco activity directly decrease boll weight in waterlogged cotton. Besides the role in Sucrose Metabolism after waterlogging, SuSy also had a positive significant correlation with the duration of rapid-accumulation period for seed fiber weight (P<0.05). These findings elucidated mechanisms to waterlogging that affected seed fiber weight, which resulted from alteration in carbohydrates, enzymes and genes.
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changes of Sucrose Metabolism in leaf subtending to cotton boll under cool temperature due to late planting
Field Crops Research, 2013Co-Authors: Jingran Liu, Youhua Wang, Zhiguo Zhou, Ji Chen, Abudukeyoumu Abudurezike, Derrick M OosterhuisAbstract:Abstract Because reproductive growth could be influenced by Sucrose Metabolism of major source leaf (leaf subtending to cotton boll, LSCB), we hypothesized that decreased temperatures under field conditions would limit morphology and biomass distributions of the whole cotton plant by decreasing photosynthesis of LSCB and inhibiting Sucrose Metabolism in LSCB. To address this hypothesis, two cotton cultivars, Kemian 1 and Sumian 15, were grown at three planting dates (25 April, 25 May and 10 June) in 2009–2011 to obtain LSCB and bolls exposed to contrasting ambient temperatures while at the same developmental stage (white flowers on the first position of 6–7th fruiting branches). Sample collection and measurement were conducted during boll development at MDTmin of 25.9 °C and 24.0 °C for the early planting date of 25 April (optimal planting date in the Yangtze River Valley), 20.4 °C and 18.4 °C for the 25 May planting date, and 16.5 °C and 16.0 °C for the 10 June planting date in 2010 and 2011, respectively. Microclimate measurements included photosynthetic active radiation, relative humidity and air temperature. Late planting decreased boll number, boll weight, LAI, total biomass and harvest index ( P Pn and Sucrose transformation rate in LSCB ( P Pn , SPS activity, Sucrose transformation rate in LSCB and boll weight under cool temperature for Sumian 15 was greater than those of Kemian 1. In addition, there was a significantly positive correlation between Pn and SPS in LSCB, as well as SPS and boll weight in 2010 and 2011 ( P Pn and SPS in LSCB were necessary to improve boll weight. However, greater boll weight does not necessarily need high Pn , SPS and SuSy activities, and great Sucrose transformation rate in LSCB.
Christine H. Foyer - One of the best experts on this subject based on the ideXlab platform.
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differential regulation of grain Sucrose accumulation and Metabolism in coffea arabica arabica and coffea canephora robusta revealed through gene expression and enzyme activity analysis
New Phytologist, 2008Co-Authors: Isabelle Privat, Chenwei Lin, S D Tanksley, Severine Foucrier, Anneke Prins, Thibaut Epalle, Magali Eychenne, Laurianne Kandalaft, Victoria Caillet, Christine H. FoyerAbstract:Summary • Coffea arabica (Arabica) and Coffea canephora (Robusta) are the two main cultivated species used for coffee bean production. Arabica genotypes generally produce a higher coffee quality than Robusta genotypes. Understanding the genetic basis for Sucrose accumulation during coffee grain maturation is an important goal because Sucrose is an important coffee flavor precursor. • Nine new Coffea genes encoding Sucrose Metabolism enzymes have been identified: Sucrose phosphate synthase (CcSPS1, CcSPS2), Sucrose phosphate phosphatase (CcSP1), cytoplasmic (CaInv3) and cell wall (CcInv4) invertases and four invertase inhibitors (CcInvI1, 2, 3, 4). • Activities and mRNA abundance of the Sucrose Metabolism enzymes were compared at different developmental stages in Arabica and Robusta grains, characterized by different Sucrose contents in mature grain. • It is concluded that Robusta accumulates less Sucrose than Arabica for two reasons: Robusta has higher Sucrose synthase and acid invertase activities early in grain development – the expression of CcSS1 and CcInv2 appears to be crucial at this stage and Robusta has a lower SPS activity and low CcSPS1 expression at the final stages of grain development and hence has less capacity for Sucrose re-synthesis. Regulation of vacuolar invertase CcInv2 activity by invertase inhibitors CcInvI2 and/or CcInvI3 during Arabica grain development is considered.
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A role for ‘futile cycles’ involving invertase and Sucrose synthase in Sucrose Metabolism of tomato fruit
Journal of Experimental Botany, 2001Co-Authors: Binh Nguyen-quoc, Christine H. FoyerAbstract:: Current concepts of the factors determining sink strength and the subsequent regulation of carbohydrate Metabolism in tomato fruit are based upon an understanding of the relative roles of Sucrose synthase, Sucrose phosphate synthase and invertase, derived from studies in mutants and transformed plants. These enzymes participate in at least four futile cycles that involve sugar transport between the cytosol, vacuole and apoplast. Key reactions are (1) the continuous rapid degradation of Sucrose in the cytosol by Sucrose synthase (SuSy), (2) Sucrose re-synthesis via either SuSy or Sucrose phosphate synthase (SPS), (3) Sucrose hydrolysis in the vacuole or apoplast by acid invertase, (4) subsequent transport of hexoses to the cytosol where they are once more converted into Sucrose, and (5) rapid synthesis and breakdown of starch in the amyloplast. In this way futile cycles of Sucrose/hexose interchange govern fruit sugar content and composition. The major function of the high and constant invertase activity in red tomato fruit is, therefore, to maintain high cellular hexose concentrations, the hydrolysis of Sucrose in the vacuole and in the intercellular space allowing more efficient storage of sugar in these compartments. Vacuolar sugar storage may be important in sustaining fruit cell growth at times when less Sucrose is available for the sink organs because of exhaustion of the carbohydrate pools in source leaves.
Graciela L Salerno - One of the best experts on this subject based on the ideXlab platform.
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the cinderella story of Sucrose hydrolysis alkaline neutral invertases from cyanobacteria to unforeseen roles in plant cytosol and organelles
Plant Science, 2010Co-Authors: Walter A Vargas, Graciela L SalernoAbstract:Over the past decades, considerable advances have been made in understanding the crucial role of Sucrose and the regulation of its Metabolism in plant life. Recent studies in cyanobacteria and the analysis of several genomic sequences point towards an ancient origin of plant Sucrose Metabolism before the cyanobacterial phylogenetic radiation. In agreement with the generally accepted cyanobacterial endosymbiotic origin of plant chloroplasts, most of the cyanobacterial genes were transferred to the nucleus and their protein products were preferentially re-imported to the plant organelle. In the case of Sucrose Metabolism, the enzymes Sucrose-phosphate synthase (SPS) and Sucrose-phosphate phosphatase (SPP), responsible of the disaccharide synthesis, and Sucrose synthase (SuS) and alkaline/neutral invertases (A/N-Inv), involved in Sucrose cleavage, appear to have a cyanobacterial origin. However, whereas SPS and SPP are likely to be exclusively localized in the cytosol of modern plant cells, SuS and A/N-Inv isoforms are distributed between the cytosol and different subcellular locations. Particularly, A/N-Invs are the least studied proteins of Sucrose catabolism. They were somewhat underestimated, and thought to play no relevant role in carbon Metabolism. However, some striking recent findings about the presence of A/N-Inv forms inside plant organelles, as well as the description of novel physiological functions, led us to re-evaluate the importance of these Cinderella enzymes. The additional roles uncovered for A/N-Invs disclose new scenarios for the interconnection between the cytosol and organelles and for complex crosstalk signalling pathways.
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origin of Sucrose Metabolism in higher plants when how and why
Trends in Plant Science, 2003Co-Authors: Graciela L Salerno, Leonardo CurattiAbstract:Since the discovery of Sucrose biosynthesis, considerable advances have been made in understanding its regulation and crucial role in the functional biology of plants. However, important aspects of this Metabolism are still an enigma. Studies in cyanobacteria and the publication of the sequences of several complete genomes have recently significantly increased our knowledge of the structures of proteins involved in Sucrose Metabolism and given us new insights into their origin and further evolution.
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Sucrose Metabolism anabaena Sucrose phosphate synthase and Sucrose phosphate phosphatase define minimal functional domains shuffled during evolution
FEBS Letters, 2002Co-Authors: Andrea Cumino, Leonardo Curatti, Laura Estela Giarrocco, Graciela L SalernoAbstract:Based on the functional characterization of Sucrose biosynthesis related proteins [SBP: Sucrose-phosphate synthase (SPS), Sucrose-phosphate phosphatase (SPP), and Sucrose synthase (SuS)] in Anabaena sp. PCC7120 and sequence analysis, we have shown that SBP are restricted to cyanobacterium species and plants, and that they are multidomain proteins with modular architecture. Anabaena SPS, a minimal catalytic SPS unit, defines a glucosyltransferase domain present in all SPSs and SuSs. Similarly, Anabaena SPP defines a phosphohydrolase domain characteristic of all SPPs and some SPSs. Phylogenetic analysis points towards the evolution of modern cyanobacterial and plant SBP from a bidomainal common ancestral SPS-like gene.
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Sucrose Metabolism in cyanobacteria Sucrose synthase from anabaena sp strain pcc 7119 is remarkably different from the plant enzymes with respect to substrate affinity and amino terminal sequence
Planta, 1999Co-Authors: Andrea C Porchia, Leonardo Curatti, Graciela L SalernoAbstract:The pathway of Sucrose Metabolism in cyanobacteria is just starting to be elucidated. The present study describes the first isolation and biochemical characterization of a prokaryotic Sucrose synthase (SS, EC 2.4.1.13). Two SS forms (SS-I and SS-II) were detected in Anabaena sp. strain PCC 7119. The isoform SS-II was purified 457-fold and its amino-terminal portion sequenced. Substrate specificity, kinetic constants, native protein and subunit molecular masses, and the effect of different ions and metabolites were studied for SS-II. Anabaena SS was shown to be a tetramer with a 92-kDa polypeptide that was recognized by maize SS polyclonal antibodies. Some striking differences from plant enzymes were demonstrated with respect to substrate affinities, regulation by metal ions and ATP, and the amino-acid sequence of the N-terminal region.
Zhenle Yang - One of the best experts on this subject based on the ideXlab platform.
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Sucrose Metabolism in developing oil rich tubers of cyperus esculentus comparative transcriptome analysis
BMC Plant Biology, 2018Co-Authors: Zhenle Yang, Dantong LiuAbstract:Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots. Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc Metabolism in underground oil-rich storage tissues. In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc Metabolism. The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc Metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc Metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development. The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway. We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue. The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc Metabolism occurred in oil tubers of C. esculentus.
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Sucrose Metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis
BMC, 2018Co-Authors: Zhenle Yang, Dantong LiuAbstract:Abstract Background Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots. Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc Metabolism in underground oil-rich storage tissues. In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc Metabolism. Results The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc Metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc Metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development. The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway. Conclusions We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue. The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc Metabolism occurred in oil tubers of C. esculentus
